| identification of indolepyruvic acid as an intermediate of rebeccamycin biosynthesis. | experimental evidence is presented to demonstrate that indolepyruvic acid is an intermediate in the rebeccamycin biosynthetic pathway. [3-14c]indolepyruvic acid was prepared and efficiently incorporated (8%) into rebeccamycin by saccharothrix aerocolonigenes. | 1990 | 1366997 |
| tetrazomine, a new antibiotic produced by an actinomycete strain. taxonomy, fermentation, isolation and characterization. | a new antibacterial antibiotic tetrazomine was found from the fermentation broth of an actinomycete strain which was isolated from beach sand collected at chichijima, ogasawara islands, tokyo, japan. the strain y-09194l, was identified as saccharothrix mutabilis subsp. chichijimaensis subsp. nov. the antibiotic exhibited broad antimicrobial activity against gram-positive and gram-negative bacteria in vitro. it also exhibited strong cytotoxic activity against p388 leukemia cells and showed antitu ... | 1991 | 2061191 |
| karnamicin, a complex of new antifungal antibiotics. i. taxonomy, fermentation, isolation and physico-chemical and biological properties. | a complex of new antifungal antibiotics designated karnamicin was isolated from the cultured broth of saccharothrix aerocolonigenes no. n806-4. fifteen components have so far been isolated from the complex; the major component karnamicin b2 was identified by x-ray crystallography to be a novel molecule unrelated to known antibiotics. all components of karnamicin exhibited a rather broad spectrum of activity against fungi and yeasts with mics ranging from 3.1 to 50 micrograms/ml. | 1989 | 2661511 |
| 3-o-isobutyrylkinamycin c and 4-deacetyl-4-o-isobutyrylkinamycin c, new antibiotics produced by a saccharothrix species. | | 1989 | 2708140 |
| production and biological activity of rebeccamycin, a novel antitumor agent. | an actinomycete, strain c-38,383, was selected in a screening program for the isolation of novel antitumor agents. a yellow crystalline product, named rebeccamycin, was isolated from the mycelium and was found to have activity against p388 leukemia, l1210 leukemia and b16 melanoma implanted in mice. rebeccamycin inhibits the growth of human lung adenocarcinoma cells (a549) and produces single-strand breaks in the dna of these cells. no dna-protein cross-links were detected. a related antibiotic, ... | 1987 | 3112080 |
| phosphonothrixin, a novel herbicidal antibiotic produced by saccharothrix sp. st-888. i. taxonomy, fermentation, isolation and biological properties. | a novel herbicidal compound, phosphonothrixin, was found in the fermentation broth of saccharothrix sp. st-888 cultured on a vegetable juice medium. the compound exhibiting acidic and hydrophilic properties was obtained when the fermentation broth of st-888 was subjected to ion exchange chromatography, gel filtration chromatography and ion-pair chromatography. phosphonothrixin significantly inhibited germination of gramineous and broadleaf weeds. foliar application of this antibiotic gave rise t ... | 1995 | 7490218 |
| phosphonothrixin, a novel herbicidal antibiotic produced by saccharothrix sp. st-888. ii. structure determination. | phosphonothrixin (1) is a novel herbicidal agent produced by saccharothrix sp. st-888. this unique compound possessing a c-p bond and an isoprene unit was determined to be 2-hydroxy-2-hydroxymethyl-3-oxobutyl-phosphonic acid. unusually rapid proton-deuterium (h-d) exchange of the methyl ketone unit of this antibiotic in d2o solution was observed. phosphonothrixin can be classified as a new type of natural product herbicide having a c-p bond in the molecule. | 1995 | 7490219 |
| total synthesis of (+/-)-phosphonothrixin, a novel herbicidal antibiotic containing c-p bond. | phosphonothrixin (1) is a novel herbicidal agent produced by saccharothrix sp. st-888, with a unique structure possessing a c-p bond and an isoprene unit. the total synthesis of this antibiotic was accomplished from methyl (bromomethyl)acrylate in six steps. | 1995 | 7490220 |
| fluvirucins a1, a2, b1, b2, b3, b4 and b5, new antibiotics active against influenza a virus. iv. taxonomy on the producing organisms. | the morphology, chemotaxonomy, and cultural and physiological characteristics were examined on the five strains of actinomycetes which produce antiviral antibiotics, fluvirucin congeners. all strains have meso-2,6-diaminopimelic acid in the cell wall. four strains, q464-31, l407-5, r359-5 and r516-16, belong to the maduromycetes since they have madurose in the whole cell. the remaining one strain, r869-90, has rhamnose but no madurose, and is a nocardioform actinomycete. these five strains were ... | 1991 | 1938616 |
| isolation of a bromo analog of rebeccamycin from saccharothrix aerocolonigenes. | when grown in a defined medium containing 0.05% kbr, saccharothrix aerocolonigenes atcc 39243 produces a novel bromo analog of rebeccamycin. this new analog, designated bromorebeccamycin, has been isolated from the culture broth and purified by vacuum liquid chromatography and column chromatography. spectroscopic data demonstrated that bromorebeccamycin has the same structure as rebeccamycin, except for the replacement of the two chlorine atoms by bromine atoms in the molecule. bromorebeccamycin ... | 1991 | 1938615 |
| a new alkaloid antibiotic tetrazomine. structure determination. | a new alkaloid antibiotic tetrazomine was isolated from the culture broth of saccharothrix mutabilis subsp. chichijimaensis subsp. nov., and its structure was determined to be i by means of spectroscopic measurements. it has an unusual structure which consists of six rings, including piperidine, piperazine, oxazole, and pyrrolidine rings. | 1991 | 1778788 |
| galacardins a and b, new glycopeptide antibiotics. | a strain of actinomycetes identified as saccharothrix sp. sank 64289 was found to produce new antibiotics, galacardins a and b. their physico-chemical properties showed that they were new members of glycopeptide antibiotics. they were structurally related to beta-avoparcin but differed from it only in sugar composition. though beta-avoparcin does not contain galactose, galacardins a and b did contain two and one moles of galactose, respectively. they showed strong antimicrobial activity against ... | 1992 | 1577657 |
| phylogenetic analysis of saccharothrix and related taxa: proposal for actinosynnemataceae fam. nov. | partial sequences for 16s rdna were determined for strains of the genus saccharothrix, including most described species, as well as strains of the described species of the related genera kutzneria, actinokineospora and actinosynnema. these were aligned with published sequences for other species of these genera, as well as those of the genera 'actinoalloteichus', 'asiosporangium', lentzea, kutzneria, streptoalloteichus and representative taxa from other actinomycete families. phylogenetic analysi ... | 2000 | 10826820 |
| revival of the genus lentzea and proposal for lechevalieria gen. nov. | the genus saccharothrix is phylogenetically heterogeneous on the basis of analysis of almost complete 16s rdna sequences. an evaluation of chemotaxonomic, morphological and physiological properties in the light of the molecular phylogeny data revealed that several species are misclassified. saccharothrix aerocolonigenes nrrl b-3298t and saccharothrix flava nrrl b-16131t constitute a lineage distinct from saccharothrix and separate from lentzea. the genus lechevalieria gen. nov. is proposed for t ... | 2001 | 11411672 |
| tmc-86a, b and tmc-96, new proteasome inhibitors from streptomyces sp. tc 1084 and saccharothrix sp. tc 1094. ii. physico-chemical properties and structure determination. | | 2000 | 10724010 |
| tmc-86a, b and tmc-96, new proteasome inhibitors from streptomyces sp. tc 1084 and saccharothrix sp. tc 1094. i. taxonomy, fermentation, isolation, and biological activities. | tmc-86a, b and tmc-96, new 20s proteasome inhibitors with an epoxy-beta-aminoketone moiety, were isolated from the fermentation broth of streptomyces sp. tc 1084 and saccharothrix sp. tc 1094, respectively. tmc-86a, b and tmc-96 inhibited the chymotrypsin-like and peptidylglutamyl-peptide hydrolyzing activities of 20s proteasome with the following ic50 values: tmc-86a, 5.1 microm and 3.7microm; tmc-86b, 1.1 microm and 31 microm; tmc-96, 2.9 microm and 3.5 microm, respectively. tmc-86a, b and tmc ... | 1999 | 10695669 |
| actinomycetes in karstic caves of northern spain (altamira and tito bustillo). | a variety of isolation procedures were carried out to study the involvement of bacteria in the colonisation and biodeterioration of spanish caves with paleolithic rock art (altamira and tito bustillo). the applied techniques mainly aimed to isolate heterotrophic bacteria such as streptomycetes, nocardioform and coryneform actinomycetes, and other gram-positive and gram-negative bacteria. the results demonstrated that actinomycetes were the most abundant gram-positive bacteria in the caves. actin ... | 1999 | 10353805 |
| pathogenicity and immunogenicity of a listeria monocytogenes strain that requires d-alanine for growth. | listeria monocytogenes is an intracellular bacterial pathogen that elicits a strong cellular immune response following infection and therefore has potential use as a vaccine vector. however, while infections by l. monocytogenes are fairly rare and can readily be controlled by a number of antibiotics, the organism can nevertheless cause meningitis and death, particularly in immunocompromised or pregnant patients. we therefore have endeavored to isolate a highly attenuated strain of this organism ... | 1998 | 9673233 |
| formamicin, a novel antifungal antibiotic produced by a strain of saccharothrix sp. ii. structure elucidation of formamicin. | a novel antifungal antibiotic, formamicin, was isolated from the culture broth of saccharothrix sp. mk27-91f2. the absolute structure of formamicin was determinated by spectroscopic and x-ray crystallographic analysis and degradation study. | 1997 | 9592566 |
| formamicin, a novel antifungal antibiotic produced by a strain of saccharothrix sp. i. taxonomy, production, isolation and biological properties. | formamicin, an antifungal antibiotic, was isolated from the cultured broth of an actinomycete strain. the strain was isolated from a soil collected at setagaya-ku, tokyo, japan, and identified as saccharothrix sp. mk27-91f2. formamicin was extracted with acetone from cultured mycelia and purified by silicagel and sephadex lh-20 column chromatographies and cpc (centrifugal liquid-liquid partition chromatography). formamicin showed strong antimicrobial activity against phytopathogenic fungi. | 1997 | 9592565 |
| a mutant d-amino acid aminotransferase with broad substrate specificity: construction by replacement of the interdomain loop pro119-arg120-pro121 by gly-gly-gly. | d-amino acid aminotransferase (ec 2.6.1.21) catalyzes the interconversion of various d-amino acids and 2-oxo acids. each homodimer subunit consists of two domains, which are connected by a single loop, asn118-pro119-arg120-pro121. the loop has no direct contact with the active site region or the cofactor, pyridoxal 5'-phosphate. we attempted to increase the conformational flexibility of this loop through a triple glycine substitution. the resultant mutant p119g-r120g-p121g has features clearly d ... | 1998 | 9579660 |
| description of lentzea flaviverrucosa sp. nov. and transfer of the type strain of saccharothrix aerocolonigenes subsp. staurosporea to lentzea albida. | a distinct actinomycete isolated from soil was subjected to polyphasic taxonomic analysis. it is demonstrated by comparative 16s rdna gene sequencing that the organism, designated strain as 4.0578t, represents a novel species of the genus lentzea. the phylogenetic results also showed that it formed a monophyletic lineage distinct from the available members of the genera lentzea, lechevalieria and saccharothrix. the organism was distinguished from all the validly described type strains of the gen ... | 2002 | 12361291 |
| new dithiolopyrrolone antibiotics from saccharothrix sp. sa 233. i. taxonomy, fermentation, isolation and biological activities. | three new natural antibacterial and antifungal dithiolopyrrolone antibiotics were isolated along with the known iso-butyropyrrothine and thiolutine from the fermentation broth of an actinomycete strain which was isolated from a saharian palm grove soil collected at adrar, south algeria. the strain was identified as saccharothrix sp. the three new antibiotics exhibited broad antimicrobial activity against gram-positive bacteria, yeasts and fungi in vitro. | 2002 | 12374382 |
| new dithiolopyrrolone antibiotics from saccharothrix sp. sa 233. ii. physicochemical properties and structure elucidation. | three new natural dithiopyrrolone antibiotics, 3-methyl-2-butenoylpyrrothine (1), tigloylpyrrothine (2), and n-butyropyrrothine (3) were isolated along with the known isobutyropyrrothine (4) and thiolutin (5) from the fermentation broth of saccharothrix sp. sa 233. the structures of the novel compounds were established on the basis on their spectral data. | 2002 | 12374383 |
| secretion of neu differentiation factor-like polypeptides by cph-transformed fibroblasts: cloning and characterization of syrian hamster neuregulin cdnas. | we reported previously that expression of the cph oncogene in syrian hamster and mouse cells leads to the secretion of a polypeptide factor or factors structurally and functionally related to neu differentiation factor (ndf) and the establishment of an autocrine loop mediated through the simultaneous overexpression of the erbb4 receptor. to identify the nature of this factor and to characterize its role in the neoplastic conversion of syrian hamster embryo cells, we cloned cdnas hybridizing to r ... | 1998 | 9537646 |
| adenosine-5'-phosphate deaminase. a novel herbicide target. | the isolation of carbocyclic coformycin as the herbicidally active component from a fermentation of saccharothrix species was described previously (b.d. bush, g.v. fitchett, d.a. gates, d. langley [1993] phytochemistry 32: 737-739). here we report that the primary mode of action of carbocyclic coformycin has been identified as inhibition of the enzyme amp deaminase (ec 3.5.4.6) following phosphorylation at the 5' hydroxyl on the carbocyclic ring in vivo. when pea (pisum sativum l. var onward) se ... | 1997 | 9159944 |
| a family of novel macrocyclic lactones, the saccharocarcins produced by saccharothrix aerocolonigenes subsp. antibiotica. ii. physico-chemical properties and structure determination. | six novel tetronic acid analogs were isolated from the fermentation broth of the actinomycete saccharothrix aerocolongenes subsp. antibiotica scc1886. the structures of these saccharocarcins were determined by their spectral data, and chemical degradation. all six compounds are derived from two modified tetronic acid homologs which differ from other tetronic acids by having an ethyl or propyl side chain at c-23 and a methyl group at c-16. they are all characterized by a novel sugar-amide at c-17 ... | 1997 | 9099221 |
| a family of novel macrocyclic lactones, the saccharocarcins produced by saccharothrix aerocolonigenes subsp. antibiotica. i. taxonomy, fermentation, isolation and biological properties. | a nocardioform actinomycete, scc 1886, isolated from a soil sample collected in ohio was found to produce, in fermentation, six novel macrocyclic lactones, the saccharocarcins. the producing culture was identified as saccharothrix aerocolonigenes subsp. antibiotica based on the formation of fragmenting substrate mycelia, aerial mycelia that coalesce to form aerial colonies, whole-cell hydrolysates that contain meso-diaminopimelic acid, galactose and rhamnose and physiological comparisons to type ... | 1997 | 9099220 |
| analogues of antifungal tjipanazoles from rebeccamycin. | analogues of antifungal tjipanazoles were obtained by semi-synthesis from rebeccamycin, an antitumor antibiotic isolated from cultures of saccharothrix aerocolonigenes. the antiproliferative activities of the new compounds were evaluated in vitro against nine tumor cell lines. the effect on the cell cycle of murine leukemia l1210 cells was examined and the antimicrobial activities against two gram positive bacteria, a gram negative bacterium and a yeast were determined. the inhibitory properties ... | 2004 | 15051063 |
| a unique enzyme from saccharothrix sp. catalyzing d-amino acid transfer. | a newly isolated actinomycete belonging to saccharothrix sp. was found to produce a unique enzyme catalyzing d-amino acid transfer. the enzyme, which was tentatively named d-amino acid transferase, was purified 2600-fold to electrophoretic homogeneity and the molecular mass was 41 kda. the enzyme was d-configuration specific and recognized aromatic d-amino acid esters to form oligo d-amino acid esters. d-phenylalanine ester was favored as substrate over other d-amino acid esters. the optimum con ... | 1997 | 9003435 |
| analysis of two capreomycin-resistance determinants from streptomyces capreolus and characterization of the action of their products. | two genes encoding capreomycin (cp)-modifying enzymes have been isolated from the producing organism streptomyces capreolus. cp acetyltransferase (cac), encoded by cac, is active against all four components of the cp complex, whereas cp phosphotransferase (cph), the product of cph, is active against cp components ia and iia (and also the related antibiotic, vm) but not against cp ib or cp iib. | 1995 | 8566762 |
| a phylogenetic analysis of the family pseudonocardiaceae and the genera actinokineospora and saccharothrix with 16s rrna sequences and a proposal to combine the genera amycolata and pseudonocardia in an emended genus pseudonocardia. | the 16s rrnas of 15 species of actinomycetes belonging to the genera actinokineospora and saccharothrix and the family pseudonocardiaceae, including amycolatopsis, amycolata, pseudonocardia, saccharomonospora, and saccharopolyspora species, were sequenced by using reverse transcriptase. the sequences were analyzed along with the sequences of reference actinomycetes by using distance matrix and parsimony methods. the wall chemotype iv genus actinokineospora was found to be closely related to spec ... | 1994 | 8186093 |
| amino acid sequence analysis of ribosomal protein at-l30 from members of the family pseudonocardiaceae. | the phylogenetic relationships of the genera belonging to the family pseudonocardiaceae were examined by a novel approach, amino acid sequencing of ribosomal at-l30 proteins. the results of partial amino acid sequencing of at-l30 preparations revealed that the members of the family pseudonocardiaceae are divided into four clusters; the first cluster contains the genus actinopolyspora, the second cluster contains the genus saccharopolyspora, the third cluster contains the genus amycolatopsis, and ... | 1995 | 7857791 |
| lentzea gen. nov., a new genus of the order actinomycetales. | we describe a new genus of mesophilic actinomycetes, for which we propose the name lentzea. the strains of this genus form abundant aerial hyphae that fragment into rod-shaped elements. whole-cell hydrolysates contain the meso isomer of diaminopimelic acid and no characteristic sugar (wall chemotype iii). the phospholipid pattern type is type pii (phosphatidylethanolamine is the characteristic phospholipid); the major menaquinone is mk-9. the fatty acid profile comprises saturated, unsaturated, ... | 1995 | 7537071 |
| ws75624 a and b, new endothelin converting enzyme inhibitors isolated from saccharothrix sp. no. 75624. ii. structure elucidation of ws75624 a and b. | the structures of ws75624 a and b, novel endothelin converting enzyme inhibitors, were determined to be 1 and 2a, respectively, by a combination of chemical evidence and a series of 2d nmr spectral analyses. | 1995 | 7490209 |
| ws75624 a and b, new endothelin converting enzyme inhibitors isolated from saccharothrix sp. no. 75624. i. taxonomy, fermentation, isolation, physico-chemical properties and biological activities. | novel endothelin converting enzyme (ece) inhibitors, ws75624 a and b, have been isolated from the fermentation broth of saccharothrix sp. no. 75624. these inhibitors were purified from an acetone extract of whole culture broth followed by hp-20 column chromatography, silica gel column chromatography and hplc. ws75624 a and b showed highly potent ece inhibitory activity, and both had ic50 values of 0.03 microgram/ml. ws75624 a and b also showed other metalloprotease (collagenase and neutral endop ... | 1995 | 7490208 |
| menaquinone composition in the classification and identification of aerobic actinomycetes. | menaquinones were the only isoprenoid quinones found in 36 strains representing different species of the genera nocardia, mycobacterium, rhodococcus, amycolatopsis, saccharothrix, streptomyces, nocardiopsis and actinomadura. dihydrogenated menaquinones with nine isoprene units [mk-9(h2)] were the main components isolated from mycobacterium. dihydrogenated and tetrahydrogenated menaquinones with eight isoprene units were the predominant compounds identified in typical rhodococcus and nocardia str ... | 1988 | 3376615 |
| the biosynthetic origins of rebeccamycin. | experimental evidence is presented to demonstrate that the antitumor-antibiotic rebeccamycin is biosynthesized by saccharothrix aerocolonigenes from one unit of glucose, one of methionine, and two of tryptophan. evidence is presented that suggests that the alpha-amino group of neither tryptophan unit provides the nitrogen of the phthalimide system. | 1988 | 3204382 |
| effect of amino acids containing sulfur on dithiolopyrrolone antibiotic productions by saccharothrix algeriensis nrrl b-24137. | to study the effect of sulfur-containing amino acids (l-cysteine, l-cystine, l-methionine and dl-ethionine) on the production of dithiolopyrrolone antibiotics by saccharothrix algeriensis nrrl b-24137. | 2006 | 16430516 |
| genes and enzymes involved in caffeic acid biosynthesis in the actinomycete saccharothrix espanaensis. | the saccharomicins a and b, produced by the actinomycete saccharothrix espanaensis, are oligosaccharide antibiotics. they consist of 17 monosaccharide units and the unique aglycon n-(m,p-dihydroxycinnamoyl)taurine. to investigate candidate genes responsible for the formation of trans-m,p-dihydroxycinnamic acid (caffeic acid) as part of the saccharomicin aglycon, gene expression experiments were carried out in streptomyces fradiae xks. it is shown that the biosynthetic pathway for trans-caffeic a ... | 2006 | 16547054 |
| saccharothrix violacea sp. nov., isolated from a gold mine cave, and saccharothrix albidocapillata comb. nov. | the generic position of two isolates from soils inside a gold mine cave in kongju, korea, was determined by 16s rdna sequencing and chemotaxonomic characteristics. phylogenetic analysis indicated that both of the isolates formed a clade with lentzea albidocapillata and members of the genus saccharothrix of the family pseudonocardiaceae. the chemical composition of the isolates and of lentzea albidocapillata was consistent with that of the genus saccharothrix, which is characterized by a type iii ... | 2000 | 10843077 |
| cloning and expression of a gene encoding n-glycosyltransferase (ngt) from saccarothrix aerocolonigenes atcc39243. | in the course of our bioconversion studies on the derivatives of an indolocarbazole, j-104303, saccharothrix aerocolonigenes atcc39243 was found to convert j-104303, which was added into the culture medium, to its glycosylated derivative, j-109384. in order to clone the gene having the ability to convert j-104303 to j-109384, a library of saccharothrix aerocolonigenes atcc39243 dna fragments was constructed using streptomyces lividans tk21 and pij702 as host strain and vector, respectively. by e ... | 2000 | 10866221 |
| saccharomicins, novel heptadecaglycoside antibiotics produced by saccharothrix espanaensis: antibacterial and mechanistic activities. | saccharomicins a and b, two new heptadecaglycoside antibiotics, were isolated from the fermentation broth of the rare actinomycete saccharothrix espanaensis. they represent a novel class of bactericidal antibiotics that are active both in vitro and in vivo against bacteria and yeast (mics: staphylococcus aureus, <0.12 to 0. 5; vancomycin-resistant enterococci, 0.25 to 16; gram-negative bacteria, 0.25 to >128; and yeast, >128 microg/ml), including multiply resistant strains. saccharomicins protec ... | 2000 | 10898690 |
| production, isolation and structure determination of novel fluoroindolocarbazoles from saccharothrix aerocolonigenes atcc 39243. | saccharothrix aerocolonigenes atcc 39243 produces an indolocarbazole antitumor agent rebeccamycin under submerged fermentation conditions. adding dl-6-fluorotryptophan to culture of s. aerocolonigenes atcc 39243 induces the formation of two novel indolocarbazoles, fluoroindolocarbazoles a and b. feeding dl-5-fluorotryptophan to culture of s. aerocolonigenes atcc 39243 induces the production of a novel indolocarbazole, fluoroindolocarbazole c. these fluoroindolocarbazoles have been isolated from ... | 2001 | 11269705 |
| crossiella gen. nov., a new genus related to streptoalloteichus. | phylogenetic analysis of the genera within the suborder pseudonocardineae based on almost complete sequences of 16s rdna showed that saccharothrix cryophilis nrrl b-16238t was misplaced within the genus saccharothrix. saccharothrix cryophilis nrrl b-16238t appeared to be phylogenetically closest to streptoalloteichus, but is morphologically distinct from this genus because sporangia with motile spores are not observed. the aerial mycelium fragments into rod-shaped elements and sclerotium-like bo ... | 2001 | 11491360 |
| ammocidin, a new apoptosis inducer in ras-dependent cells from saccharothrix sp. i. production, isolation and biological activity. | a new apoptosis inducer, ammocidin, was isolated from the culture broth of saccharothrix sp. aj9571. ammocidin induced apoptotic cell death in ras-dependent ba/f3-v12 cells with an ic50 of 66 ng/ml. no cell death was observed in il-3-dependent ba/f3-v12 cells at less than 100 microg/ml of ammocidin. ammocidin significantly reduced the phosphorylation level of mapk and s6k that mediate the anti-apoptotic function of ras. | 2001 | 11714226 |
| ammocidin, a new apoptosis inducer in ras-dependent cells from saccharothrix sp. ii. physico-chemical properties and structure elucidation. | the structure of ammocidin, a new apoptosis inducer in ras-dependent cells from saccharothrix sp. aj9571, was elucidated to be as shown in fig. 1 by nmr and degradation studies. ammocidin consists of a 20-membered macrolide ring and three deoxy sugars identified as 6-deoxy-l-glucose, d-digitoxose and d-olivomycose. | 2001 | 11714227 |
| pluraflavins, potent antitumor antibiotics from saccharothrix sp. dsm 12931. | the new pluramycin-type antibiotics pluraflavin a, c43h54n2o14, pluraflavin b, c43h56n2o15, and pluraflavin e, c36h41no14 were isolated from cultures of the saccharothrix species dsm 12931. the structures of the novel compounds were elucidated with the aid of 2d nmr and mass spectrometric investigations. the characteristic structural element of pluraflavins a and b is an additional 4-epi-vancosamine unit at position 13 of the anthraquinone-gamma-pyrone ring system. pluraflavin e has a carboxyl g ... | 2001 | 11714228 |
| a new type of aminoacyltransferase from saccharothrix sp. as-2 favorable for the synthesis of d-amino acid-containing peptides. | a unique enzyme with some properties favorable for the synthesis of d-amino acid-containing peptides has been purified from the culture broth of saccharothrix sp. as-2. the purification steps included ammonium sulfate fractionation, chromatographies on cm-toyopearl 650m and protex butyl, and sucrose density-gradient isoelectric focusing. the enzyme, consisting of four subunits of 56 kda, showed its maximum transfer activity at around ph 8.2 and 35 degrees c, and had an isoelectric point of 5.8. ... | 2002 | 11820939 |
| the biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives. | rebeccamycin, a halogenated natural product of the indolocarbazole family, is produced by saccharothrix aerocolonigenes atcc39243. several rebeccamycin analogues, which target dna topoisomerase i or ii, have already entered clinical trials as anticancer drugs. using as a probe an internal fragment of ngt, a saccharothrix aerocolonigenes gene encoding an indolocarbazole n-glycosyltransferase, we isolated a dna region that directed the biosynthesis of rebeccamycin when introduced into streptomyces ... | 2002 | 11983340 |
| new angucyclines and antimicrobial diketopiperazines from the marine mollusk-derived actinomycete saccharothrix espanaensis an 113. | two new angucyclines, saccharothrixmicines a (2) and b (3), together with three known diketopiperazines 4-6 were isolated from the actinomycete saccharothrix espanaensis an 113 associated with the marine mollusk anadara broughtoni. their structures were determined by hresi-ms and 1d and 2d nmr. compounds 2 and 3 differ in aglycone and glycosidic bond type. 2 is an alpha-l-6-deoxyaltrose-phenylglycoside of a benz[a]anthraquinone aglycone, while 3 is an o-glycoside of the same sugar linked to c-7 ... | 2010 | 20433079 |
| new genus-specific primers for the pcr identification of novel isolates of the genera nocardiopsis and saccharothrix. | the taxonomic position of the genera saccharothrix and nocardiopsis has evolved in recent years to accommodate an increasing number of actinomycete strains that cannot be clearly distinguished by morphological characters. more recently, the taxonomic reorganization of the genus saccharothrix has determined the creation of new, related genera, increasing the complexity of the identification of this taxon. nevertheless, today these genera can still only be identified by applying chemotaxonomic and ... | 2002 | 12148658 |
| [a procedure for developing selective isolation of pseudonocardiacetes from soil samples]. | pseudonocardiacetes are a group of rare-actinomyces with type iv cell walls and without mycolic acids. five different selective isolation media s1, s2, s3, s4 and s5 were devised and practiced to improve their selective isolation efficiency. the media were detected by 33 type strains of pseudonocardiaceae, 4 strains of saccharothrix and 15 type strains of streptomyces cultured on them. soil samples were examined on media s1 ad s2 compared with glucose-asparagine agar. it shows that media s1 and ... | 2000 | 12548963 |
| [study on the reclassification of two deposited strains as4.1186 and as4.1187 of nocardia]. | polyphasic evidence for the reclassification of nocardia nostocoides as4.1186 and nocardia salmonicolor var. aurantiaca as4.1187 indicated that strain as4.1186 was closely related to saccharothrix texasensis and strain as4.1187 was very closely related to rhodococcus ruber. binary 16s rdna sequence for the pair as4.1186 and saccharothrix texasensis nrrl b-16134t, and the pair as4.1187 and rhodococcus ruber dsm 43338t exhibited 99.3% and 99.5% similarity, respectively. corresponding dna-dna reass ... | 2002 | 12557341 |
| [the application of succession analysis in combination with extremely high-frequency irradiation to the selective isolation of actinomycetes from soil]. | a new method employing succession analysis and extremely high-frequency (ehf) irradiation is proposed for the selective isolation of actinomycetes from soil. total actinomycetes were efficiently isolated from soil suspensions irradiated in the wavelength band 4.6-5.8 mm on the 14th and 45th days of succession initiated by soil wetting and from soil suspensions irradiated in the wavelength band 8-11.5 mm on the 7th day of succession. the rare actinomycete genera actinomadura, micromonospora, nono ... | 2003 | 12698804 |
| osccamk genotype determines bacterial communities in rice roots under paddy and upland field conditions. | the effects of osccamk genotype (dominant homozygous, d; heterozygous, h; recessive homozygous, r) on rice root-associated bacteria, including endophytes and epiphytes, were examined by using a tos17 rice mutant line under paddy and upland field conditions. roots were sampled at flowering stage and were subjected to clone library analyses. the relative abundance of alphaproteobacteria was noticeably decreased in r plants under both paddy and upland conditions (0.8% and 3.0%, respectively) relati ... | 2011 | 21551283 |
| biodegradation of poly(l-lactide). | the biodegradation of poly(l-lactide) (pla) is reviewed. the important role of actinomycetes in pla degradation is emphasized. these pla-degrading actinomycetes belong phylogenetically to the pseudonocardiaceae family and related genera, including amycolatopsis, lentzea, streptoalloteichus, kibdelosporangium and saccharothrix. a pla-degrading enzyme purified from an isolated amycolatopsis strain-41 has substrate specificity on pla higher than proteinase k. the application of these strains and th ... | 2004 | 15274245 |
| ammocidins b, c and d, new cytotoxic 20-membered macrolides from saccharothrix sp. aj9571. | ammocidins b, c and d were isolated from the culture broth of saccharothrix sp. aj9571, an ammocidin a-producing strain. their structures were determined by a detailed spectroscopic analysis and by a comparison of their nmr data with those of ammocidin a. ammocidins a and b showed potent anti-proliferative activities against human cancer cell lines. | 2009 | 19198629 |
| isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities. | this study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell anadara broughtoni inhabiting in the sea of japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. a total of 149 strains were isolated and identified phenotypically. a total of 27 strains were selected to be investigated phylogenetically by 165 rrna gene sequence analysis. the most bacteria were aff ... | 2008 | 19216104 |
| saccharothrix violaceirubra sp. nov., isolated from soil and plant litter. | two strains of nocardioform actinomycetes, isolated from soil and plant litter in yamanashi prefecture, japan, showed substrate mycelium with purple to dark-red colours. 16s rrna gene sequence analysis indicated that the organisms belonged to the family actinosynnemataceae and were related closely to saccharothrix strains (96.7-98.0 % sequence similarity). the isolates contained mk-9(h(4)) as the predominant menaquinone, meso-diaminopimelic acid, galactose as the diagnostic whole-cell sugar and ... | 2009 | 19502343 |
| expression of pyrrothine n-acyltransferase activities in saccharothrix algeriensis nrrl b-24137: new insights into dithiolopyrrolone antibiotic biosynthetic pathway. | the hypothetical dithiolopyrrolone biosynthetic pathway includes a final step of pyrrothine nucleus acylation. the presence of an enzymatic activity catalysing this reaction was investigated in saccharothrix algeriensis nrrl b-24137. to understand the effect exerted by organic acids on the level of dithiolopyrrolone production, their influence on enzymatic expression was studied. | 2009 | 19761464 |
| reclassification of 'dactylosporangium variesporum' as saccharothrix variisporea corrig. (ex tomita et al. 1977) sp. nov., nom. rev. | in the course of a polyphasic study it was observed that 'dactylosporangium variesporum' nrrl b-16296 is misclassified in the genus dactylosporangium as it exhibits properties consistent with its assignment to the genus saccharothrix. phylogenetic analyses based on 16s rrna gene sequences show that the strain falls within the evolutionary radiation of the genus saccharothrix, a result which is supported by corresponding chemotaxonomic and morphological markers. the strain is phylogenetically mos ... | 2011 | 20305067 |
| dithiolopyrrolone antibiotic formation induced by adding valeric acid to the culture broth of saccharothrix algeriensis. | three new antibiotics were isolated from the fermentation broth of saccharothrix algeriensis nrrl b-24137 and characterized as the dithiolopyrrolone derivatives valerylpyrrothine (1), isovalerylpyrrothine (2), and formylpyrrothine (3) as well as the known antibiotic aureothricin. the production of the dithiolopyrrolone derivatives was induced by adding valeric acid to the culture medium. the compounds exhibited moderate antimicrobial activity in vitro. | 2010 | 20507156 |
| optimization of the fermentation process of actinomycete strain hhs.015. | strain hhs.015(t) (saccharothrix yanglingensis sp. nov.), an antagonistic endophytic saccharothrix actinomycete isolated from roots of cucumber plants, exhibited a broad antimicrobial spectrum in vitro and was active as a biocontrol against plant diseases in field trials. the ssy medium was used for production of antimicrobial metabolites by strain hhs.015(t). however, this medium is too expensive for large-scale production. in this study, an alternative culture medium, based on agricultural was ... | 2010 | 20508842 |
| new dithiolopyrrolone antibiotics induced by adding sorbic acid to the culture medium of saccharothrix algeriensis nrrl b-24137. | dithiolopyrrolone antibiotics, produced by several microorganisms, are known for their strong antimicrobial activities. this class of antibiotics generated new interest after the discovery of their anticancer and antitumor properties. in this study, four new antibiotics were purified from the fermentation broth of saccharothrix algeriensis nrrl b-24137 and characterized as dithiolopyrrolone derivatives. these new dithiolopyrrolone antibiotics were induced by adding sorbic acid, as precursor, at ... | 2011 | 21323983 |
| biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in escherichia coli. | biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid class of compounds. in this study, engineered escherichia coli containing artificial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were establis ... | 2011 | 21424580 |
| poly(l-lactide) degradation by saccharothrix waywayandensis. | poly(l-lactide) (pla) was degraded by more than 95 mg from 100 mg pla film by an actinomycete, saccharothrix waywayandensis, growing in 100 ml liquid culture containing 0.1% (w/v) gelatin. in addition to degrading pla, this strain assimilated the major degradation product of pla, l-lactic acid. | 2003 | 12882561 |
| saccharothrix algeriensis sp. nov., isolated from saharan soil. | the taxonomic position of a soil isolate, strain sa 233t, recovered from saharan soil from algeria was established using a polyphasic approach. this isolate has been previously reported to produce three novel dithiolopyrrolone antibiotics, and preliminary chemotaxonomic and morphological characteristics suggested that it was representative of a member of the genus saccharothrix. phylogenetic analysis of the strain from 16s rdna sequences, along with a detailed analysis of morphological, chemotax ... | 2004 | 15280317 |
| mutactimycin pr, a new anthracycline antibiotic from saccharothrix sp. sa 103. i. taxonomy, fermentation, isolation and biological activities. | in the course of screening for new antibacterial agents, a new isolate collected from a soil sample of an arid area in south algeria, produced a red pigment which was shown an antagonistic action against a gram-positive bacterium bacillus subtilis. the isolate was identified as saccharothrix sp. and named sa 103. the red pigment, eluted by hplc on reverse phase c18 column, contained two compounds of an anthracycline antibiotics group. the structure of the major product (2) was characterized as m ... | 2004 | 15323124 |
| mutactimycin pr, a new anthracycline antibiotic from saccharothrix sp. sa 103. ii. physico-chemical properties and structure elucidation. | a new antibiotic termed mutactimycin pr (1) was isolated along with the known mutactimycin c (2) from the fermentation broth of saccharothrix sp. sa 103. the two compounds belong to the anthracycline group. the structure of these antibiotics was elucidated with the aid of nmr and mass spectrometric investigations. the novel compound mutactimycin pr was characterized as 5,12 naphtacenedione, 7-[(6-deoxy-3-o-methyl-alpha-l-mannopyranosyl)oxy]-4-[(6-deoxy-alpha-l-mannopyranosyl)oxy]-7,8,9,10-tetrah ... | 2004 | 15323125 |
| saccharothrix xinjiangensis sp. nov., a pyrene-degrading actinomycete isolated from tianchi lake, xinjiang, china. | a pyrene-degrading isolate, strain pyx-6(t), was obtained from tianchi lake, xinjiang uygur autonomous region, china, and was identified by means of polyphasic taxonomy. on the basis of 16s rrna gene sequence similarity, strain pyx-6(t) was closely related to members of the genus saccharothrix. its 16s rrna gene sequence similarity to each of saccharothrix syringae, saccharothrix australiensis, saccharothrix algeriensis and saccharothrix coeruleofusca was 98 %. chemotaxonomic and physiological p ... | 2004 | 15545440 |
| cytotoxicities of three rebeccamycin derivatives in the national cancer institute screening of 60 human tumor cell lines. | among the biologically active indolocarbazoles, rebeccamycin, a microbial metabolite produced by saccharothrix aerocolonigenes, is a well-known topoisomerase (topo) i poison. in the course of structure-activity relationship studies on rebeccamycin analogs, we have prepared a large number of indolocarbazole derivatives and have shown that, depending on the structural modifications, the cytotoxic effects may be, or not, directly correlated to dna binding and topo i inhibition. this suggests that i ... | 2005 | 15655411 |
| mutation of tlya confers capreomycin resistance in mycobacterium tuberculosis. | capreomycin, an important drug for the treatment of multidrug-resistant tuberculosis, is a macrocyclic peptide antibiotic produced by saccharothrix mutabolis subspecies capreolus. the basis of resistance to this drug was investigated by isolating and characterizing capreomycin-resistant strains of mycobacterium smegmatis and mycobacterium tuberculosis. colonies resistant to capreomycin were recovered from a library of transposon-mutagenized m. smegmatis. the transposon insertion site of one muta ... | 2005 | 15673735 |
| a lipomannan variant with strong tlr-2-dependent pro-inflammatory activity in saccharothrix aerocolonigenes. | lipomannans (lms) are powerful pro-inflammatory lipoglycans found in mycobacteria and related genera, however the molecular bases of their activity are not fully understood. we report here the isolation and the structural and functional characterization of a new lipomannan variant present in the pseudonocardineae, saccharothrix aerocolonigenes, designated saelm. using a range of chemical degradations, nmr experiments, and mass spectrometry analyses, saelm revealed a mannosylphosphatidyl-myo-inos ... | 2005 | 15955814 |
| nocardiopsis and saccharothrix genera in saharan soils in algeria: isolation, biological activities and partial characterization of antibiotics. | twenty-five soil samples were collected in the algerian sahara and analyzed to isolate rare actinomycetes. eighty-six isolates with the same nocardiopsis or saccharothrix morphology were isolated on humic-vitamin b agar medium using dilution techniques and several antibiotics as selective agents. certain of these antibiotics seemed to be very selective for some phenotypes. morphological and chemotaxonomic characteristics led to identifying 54 isolates belonging to the nocardiopsis genus and 32 i ... | 2005 | 16081249 |
| goodfellowia gen. nov., a new genus of the pseudonocardineae related to actinoalloteichus, containing goodfellowia coeruleoviolacea gen. nov., comb. nov. | during the course of a phylogenetic evaluation of saccharothrix strains held in the ars culture collection, it was discovered that saccharothrix coeruleoviolacea nrrl b-24058(t) is unrelated to other species within this genus, and a polyphasic study was undertaken to clarify its taxonomic position. strain nrrl b-24058(t) is observed to be phylogenetically separate from the genus saccharothrix and is most closely related to the genus actinoalloteichus. the strain exhibits chemotaxonomic propertie ... | 2006 | 16738092 |
| biodegradability and biodegradation of poly(lactide). | poly(lactide) (pla) has been developed and made commercially available in recent years. one of the major tasks to be taken before the widespread application of pla is the fundamental understanding of its biodegradation mechanisms. this paper provides a short overview on the biodegradability and biodegradation of pla. emphasis is focused mainly on microbial and enzymatic degradation. most of the pla-degrading microorganisms phylogenetically belong to the family of pseudonocardiaceae and related g ... | 2006 | 16823551 |
| identification of the biosynthetic gene cluster and an additional gene for resistance to the antituberculosis drug capreomycin. | capreomycin (cmn) belongs to the tuberactinomycin family of nonribosomal peptide antibiotics that are essential components of the drug arsenal for the treatment of multidrug-resistant tuberculosis. members of this antibiotic family target the ribosomes of sensitive bacteria and disrupt the function of both subunits of the ribosome. resistance to these antibiotics in mycobacterium species arises due to mutations in the genes coding for the 16s or 23s rrna but can also arise due to mutations in a ... | 2007 | 17496129 |
| proposal of umezawaea gen. nov., a new genus of the actinosynnemataceae related to saccharothrix, and transfer of saccharothrix tangerinus kinoshita et al. 2000 as umezawaea tangerina gen. nov., comb. nov. | in the course of phylogenetic analyses of the taxa within the suborder pseudonocardineae, it was observed that saccharothrix tangerinus mk27-91f2(t) was misplaced in the genus saccharothrix. after a detailed examination of nucleotide signatures in the 16s rrna gene sequence along with the morphological and chemotaxonomic characteristics of this strain, which are different from those of all species of saccharothrix as well as the other genera within the suborder, it was concluded that this strain ... | 2007 | 18048721 |
| investigations of saccharothrix algeriensis growth on synthetic media. | development of bacterial resistance to antibiotics has lead to investigations of rare bacteria, which produce new bioactive molecules. saccharothrix algriensis has been isolated from the desert maghreb. it produces dithiolopyrrolones, some of which were newly identified. in order to optimize and control production of dithiolopyrrolones, investigation regarding microorganism metabolism was required. growth on semisynthetic medium containing 2 g x l(-1) of yeast extract was complicated because it ... | 2008 | 18804057 |
| Saccharothrix yanglingensis sp. nov., an antagonistic endophytic actinomycete isolated from cucumber plant. | An endophytic actinomycete strain, designated Hhs.015(T), was isolated from roots of cucumber seedlings. The endophytic isolate was identified by means of a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence similarities, strain Hhs.015(T) was closely related to members of the genus Saccharothrix. DNA-DNA hybridization with the four closest relatives, Saccharothrix longispora NRRL B-16116(T), Saccharothrix xinjiangensis NRRL B-24321(T), Saccharothrix au ... | 2012 | 21892613 |