| taxonomic status of kitasatosporia, and proposed unification with streptomyces on the basis of phenotypic and 16s rrna analysis and emendation of streptomyces waksman and henrici 1943, 339al. | species classified within the genus kitasatosporia share many of the phenotypic characteristics typical of streptomycetes. by using a probabilistic identification scheme, they were identified with streptomyces exfoliatus cluster 5, a species group within streptomyces. the four species studied hybridized with a 16s rrna genus probe for streptomyces spp., indicating a close relationship between the two genera. the kitasatosporias were resistant to selected polyvalent streptomycete phages tested. q ... | 1992 | 1371059 |
| taxonomic properties of five kitasatosporia strains isolated by a new method. | kitasatosporia strains were found to be resistant to an antibiotic, novobiocin. by a new method using novobiocin-containing agar medium, five kitasatosporia strains, sk-3255, om-5023, sk-3406, sk-3412 and sk-3421 were efficiently isolated from soils. these five strains were characterized in common by rectiflexibiles, spores cylindrical with smooth surfaces, and no melanin formation. two strains, sk-3255 and om-5023 were taxonomically different from known species of the genus kitasatosporia, whil ... | 1991 | 1811110 |
| kifunensine inhibits glycoprotein processing and the function of the modified ldl receptor in endothelial cells. | kifunensine is an alkaloid that is produced by the actinomycete kitasatosporia kifunense and resembles the cyclic oxamide derivative of 1-aminodeoxymannojirimycin in structure. we previously showed that this compound was a potent inhibitor of the purified glycoprotein processing enzyme, mannosidase i, and caused an almost complete inhibition in the formation of complex types of oligosaccharides with the concurrent accumulation of n-linked oligosaccharides having man9(glcnac)2 structures in influ ... | 1991 | 1898016 |
| kifunensine, a potent inhibitor of the glycoprotein processing mannosidase i. | kifunensine, produced by the actinomycete kitasatosporia kifunense 9482, is an alkaloid that corresponds to a cyclic oxamide derivative of 1-amino mannojirimycin. this compound was reported to be a weak inhibitor of jack bean alpha-mannosidase (ic50 of 1.2 x 10(-4) m) (kayakiri, h., takese, s., shibata, t., okamoto, m., terano, h., hashimoto, m., tada, t., and koda, s. (1989) j. org. chem. 54, 4015-4016). we also found that kifunensine was a poor inhibitor of jack bean and mung bean aryl-alpha-m ... | 1990 | 2144287 |
| two new carbapenem antibiotic-producing actinomycetes: kitasatosporia papulosa sp. nov. and kitasatosporia grisea sp. nov. | two new carbapenem antibiotic-producing actinomycetes, the cell-walls of which contain ll-diaminopimelic acid and meso-diaminopimelic acid, were isolated from soil. the two strains were subjected to taxonomic studies, which involved morphological, cultural, physiological and chemotaxonomical characterization, the latter including the cell-wall chemo-type, whole-cell sugar composition, phospholipid composition, menaquinone system and dna base composition. these strains were identified as new spec ... | 1989 | 2921223 |
| taxonomic studies on kitasatosporia cystarginea sp. nov., which produces a new antifungal antibiotic cystargin. | taxonomic studies on a new species, kitasatosporia cystarginea are presented. among the several species already described in this genus, this strain is characteristic in forming distinct spirals of spore chains. a significant properties of the species is the production of a new antifungal antibiotic, cystargin. | 1988 | 3209468 |
| a new antifungal antibiotic, cystargin: fermentation, isolation, and characterization. | a new sulfur-containing peptide antifungal antibiotic, cystargin, was isolated from the fermentation broth of a new species of genus kitasatosporia, designated as kitasatosporia cystarginea. on acid hydrolysis, cystargin (c60h77n19o17s6) gave equimolar glycine, proline, aspartic acid and arginine. by performic acid oxidation, cysteic acid was detected after hydrolysis. it showed a growth inhibitory activity against various phytopathogenic fungi and inhibition of beta-1,3-glucan synthetase from s ... | 1988 | 3209469 |
| a novel naturally occurring carbapenem antibiotic, ab-110-d, produced by kitasatosporia papulosa novo sp. | | 1988 | 3260232 |
| structure-activity relationship studies on selected iso-alpha-carbolines. i. | in the course of microbial transformation of the antitumor compounds, it has been found that iso-alpha-carbolines and their certain derivatives undergo n-1 methylation by kitasatosporia setae. the resulting products, iso-alpha-carbolines exhibit antibacterial and antifungal properties in the concentration range of 0.2-2.5 mumol/ml. in the cytotoxicity test only derivatives substituted at c-2 and c-4 display moderate activity against kb cells. none of the compounds tested show a significant inhib ... | 1986 | 3296992 |
| microbial transformation of azacarbazoles. iii. conversion of methoxy- and phenyl- substituted alpha-carbolines to corresponding alpha-iso-carbolines by kitasatosporia setae strain. | microbial n-1 methylation of alpha-carboline derivatives substituted at position c-2, c-6 and c-8 with methoxy- and at position c-2 and c-6 with phenyl groups conducted with kitasatosporia setae resulted in corresponding alpha-iso-carboline formation. the yield of obtained products is significantly dependent on the position of substituent in alpha-carboline molecule. compounds, as 2- and 6-methoxy-alpha-carboline undergo n-1 microbial methylation with strikingly low yields of 5%, whereas product ... | 1987 | 3447525 |
| microbial transformation of azacarbazoles. iv. conversion of chloro-substituted alpha-carbolines by kitasatosporia setae strain. | biotransformation of alpha-carboline derivatives substituted at positions c-5, c-6, c-7 and c-8 with chlorine, carried out with kitasatosporia setae strain yielded corresponding 1-methyl-alpha-iso-carbolines. the formation of products is dependent on the position of chlorine in substrate molecule. when chlorine is introduced at c-6, the yield of n-1 methylation is low, about 5%. derivatives of alpha-carboline substituted with chlorine at c-7 and c-8 form corresponding alpha-iso-carbolines with y ... | 1987 | 3447526 |
| microbial transformation of azacarbazoles. v. studies on n-1 methylation of 4-methyl-alpha-carboline by kitasatosporia setae strain. | n-1 methylation of an azacarbazole, 4-methyl-alpha-carboline by kitasatosporia setae was associated with antifungal metabolite production. incubation of 4-methyl-alpha-carboline with intact cells or homogenates of kitasatosporia setae and l-/14ch3/-methionine or adenosyl-s-/14ch3/-l-methionine yielded radioactive products, 1,4-dimethyl-alpha-iso-carboline. the presence of 14c-labelled methyl groups in antifungal metabolite has been also observed. mutants of kitasatosporia setae with lost abiliti ... | 1987 | 3447527 |
| microbial transformation of azacarbazoles. vii. antitumor properties of benzo-alpha-iso-carbolines formed by kitasatosporia setae strain from corresponding benzo-alpha-carbolines. | 2,3-benzo-alpha-carboline, 7,8-benzo-alpha-carboline and their 4-methyl derivatives were subjected to microbial conversion yielding corresponding benzo-alpha-iso-carbolines. all obtained products showed significant antimicrobial and cytotoxic properties. id50 values were found to be at range 0.01-0.001 microm/ml, regarding in vitro kb tumor cells system data. it has been found that introduction of methyl group at para-position to the nitrogen of pyridine nucleus strongly increases cytotoxic and ... | 1987 | 3447529 |
| microbial transformation of azacarbazoles. viii. production of 14c-labelled benzo-alpha-iso-carbolines by permeabilized cells of kitasatosporia setae strain. | n-1 methylation of benzo-alpha-carbolines performed with kitasatosporia setae strain yields corresponding benzo-alpha-iso-carbolines, compounds demonstrating relatively strong cytostatic and antitumor properties. to obtain radioactive benzo-alpha-iso-carbolines required for the further studies of their mode of antitumor action, a simple and useful method of the preparation of 14c-labelled compounds by microbial means has been elaborated. | 1987 | 3447530 |
| kitasatosporia setae, an interesting actinomycete strain isolated from spitsbergen soil. | the phenotypic and genotypic properties as well as cell chemistry of an interesting actinomycete, which converted certain azacarbazoles into highly cytotoxic derivatives, were established. the strain was also compared with two similar japanese actinomycetes of kitasatosporia gen. nov. a great resemblance between the strains was observed. apart from similar phenotypic properties they were characterized by contents of both l- and meso-dap, glycine and galactose as the main cell wall components. th ... | 1987 | 3447538 |
| microbial transformation of azacarbazoles. i. n-methylation of alpha-, beta-, and gamma-carbolines by kitasatosporia setae strain. | microbial transformation of selected azacarbazoles, compounds noted for their activity as antitumor agents, conducted with kitasatosporia setae strain resulted in n-methylation of pyridine nucleus of alpha-, beta- and gamma-carboline molecule. formed quaternized intermediates were converted in alkaline conditions into corresponding iso-carbolines. the structure of the final products, alpha-, beta- and gamma-iso-carbolines have been established on the basis of spectral data and confirmed by chemi ... | 1987 | 3447539 |
| microbial transformation of azacarbazoles. ii. conversion of methyl-substituted alpha-carbolines to corresponding alpha-iso-carbolines by kitasatosporia setae strain. | alpha-carboline derivatives substituted with methyl groups at c-2, c-4, c-2,4, c-6 and c-8 position were subjected to n-1 methylation performed by kitasatosporia setae. the yield of formed corresponding alpha-iso-carbolines varied, depending on the position of methyl substituent. it has been observed that the methyl group introduced in position c-2 and c-6 of alpha-carboline molecule markedly diminishes the susceptibility of nitrogen for enzymic attack in comparison with methylation reaction per ... | 1987 | 3447540 |
| propioxatins a and b, new enkephalinase b inhibitors. i. taxonomy, fermentation, isolation and biological properties. | a soil isolate of actinomycete, strain sank 60684, was found to produce new enkephalinase b inhibitors, propioxatins a and b. the presence of both ll- and meso-2,6-diaminopimelic acid, glycine and galactose in the cell wall assigned this strain to genus kitasatosporia. from the morphological, cultural and physiological characteristics, this strain was determined to be kitasatosporia setae. the ki values of propioxatins a and b were 1.3 x 10(-8)m and 1.1 x 10(-7)m, respectively, for enkephalinase ... | 1986 | 3536826 |
| a new immunomodulator, fr-900494: taxonomy, fermentation, isolation, and physico-chemical and biological characteristics. | fr-900494 is a new type of immunoactive substances produced by an actinomycete named kitasatosporia kifunense sp. nov. fr-900494 exhibits a competitive action against immunosuppressive factor produced in the serum of tumor bearing mice and has the capacity to restore the depression of lymphocytes. | 1987 | 3610820 |
| kitasatosporia, a genus of the order actinomycetales. | | 1987 | 3669499 |
| propioxatins a and b, new enkephalinase b inhibitors. ii. structural elucidation. | the structures of propioxatins a (c17h29n3o6) and b (c18h31n3o6), new enkephalinase b inhibitors produced by kitasatosporia setae sank 60684, were determined. both propioxatins consist of n-acyl-l-prolyl-l-valine. n-acyl moieties of propioxatins a and b were alpha-propyl and alpha-isobutyl succinic acid beta-hydroxamic acid, respectively. | 1986 | 3781907 |
| propioxatins a and b, new enkephalinase b inhibitors. iii. total synthesis of propioxatin a. | propioxatin a, a potent enkephalinase b inhibitor produced by kitasatosporia setae sank 60684, was synthesized. the synthetic route involved a regio-selective synthesis of o-benzyl-alpha-propylsuccinic acid monohydroxamic acid via the acid chloride of alpha-propylsuccinic acid. the stereoisomer of the n-acyl moiety of natural propioxatin a was analyzed by x-ray crystallography in the form of the di-o-benzyl ester and was determined as s. devalyl propioxatin a synthesized by the same method showe ... | 1986 | 3781908 |
| isolation and characterization of terpentecin, a new antitumor antibiotic. | a new antitumor antibiotic, terpentecin was isolated from the culture broth of strain mf730-n6. strain mf730-n6, isolated from soil, was found to belong to the genus kitasatosporia. the antibiotic was extracted with chloroform, purified by column chromatography using silica gel and diaion hp-20 successively, and finally purified by high performance reverse-phase thin layer chromatography. the molecular formula of terpentecin was determined to be c20h28o6 (molecular weight, 364). the antibiotic i ... | 1985 | 3841535 |
| phosalacine, a new herbicidal antibiotic containing phosphinothricin. fermentation, isolation, biological activity and mechanism of action. | phosalacine, a new herbicidal antibiotic containing phosphinothricin was isolated from the culture filtrate of a soil isolate kitasatosporia phosalacinea ka-338. it was a water soluble, amphoteric compound obtained as an amorphous powder (c14h28n3o6p, mw 365). the antibiotic exhibited antimicrobial activity against gram-positive and gram-negative bacteria and some fungi on a minimal medium and the activity was reversed by l-glutamine. it also showed herbicidal activity against alfalfa. it is sug ... | 1984 | 6480502 |
| kitasatosporia, a new genus of the order actinomycetales. | the morphological, cultural, physiological and biochemical characteristics of a new actinomycete strain producing a new antibiotic, setamycin are described. the strain forms aerial mycelia. there is no fragmentation of vegetative mycelia. since the cell wall type is a new one containing both ll- and meso-2,6-diaminopimelic acid, glycine and galactose, strain km-6054 could not be classified in any previously named genera of the order actinomycetales. thus, it is considered to be a member of a new ... | 1982 | 7142001 |
| terpentecin and ect4b, new family of topoisomerase ii targeting antitumor antibiotics produced by streptomyces: producing organism, fermentation and large scale purification. | terpentecin and uct4b are new family of antitumor antibiotics with topoisomerase ii mediated dna cleavage activity. based on the taxonomic studies, the producing strain s-464 was identified as streptomyces sp. this strain is different from kitasatosporia griseola which had been identified as the terpentecin-producing strain in 1988. fermentation studies showed that natural nitrogen sources supported higher titer of terpentecin, and the synthetic medium with inorganic nitrogen sources supported s ... | 1995 | 7730154 |
| cloning, nucleotide sequence, and expression of an isovaleryl pepstatin-insensitive carboxyl proteinase gene from pseudomonas sp. 101. | a unique carboxyl proteinase (ec 3.4.23.33), insensitive to the classical inhibitor isovaleryl pepstatin and isolated from pseudomonas sp. 101 (pcp), is the first example of a prokaryotic enzyme of this class. the gene coding for pcp was cloned, sequenced, and expressed in escherichia coli. the gene consists of 1,761 base pairs encoding a protein of 587 amino acid residues. the nh2-terminal 215-amino acid preprosequence flanks the 372-amino acid mature protein, which is identical with the primar ... | 1994 | 7929375 |
| a taxonomic review of the genera kitasatosporia and streptoverticillium by analysis of ribosomal protein at-l30. | an analysis of the ribosomal at-l30 proteins from 42 strains of 35 species belonging to the genera streptomyces, streptoverticillium, and kitasatosporia and related genera revealed that all of the members of the genera streptoverticillium and kitasatosporia examined had the same sequence as streptomyces exfoliatus or a highly homologous sequence and exhibited high levels of relatedness to streptomyces lavendulae. these results strongly support the previous suggestion of witt and stackebrandt (d. ... | 1994 | 8186092 |
| cloning and expression of an isovaleryl pepstatin-insensitive carboxyl proteinase gene from xanthomonas sp. t-22. | xanthomonas carboxyl proteinase (xcp), isolated from xanthomonas sp. t-22, is the second example of the unique carboxyl proteinases [ec 3.4.23.33] which are insensitive to the classical aspartic proteinase inhibitor. the gene coding for xcp was cloned, sequenced, and expressed in escherichia coli. the xcp gene contains an open reading frame of 2,481 base pairs encoding a protein of 827 amino acid residues with a m(r) of 83,677. the xcp was synthesized as a large precursor consisting of three reg ... | 1996 | 8902622 |
| a proposal to revive the genus kitasatospora (omura, takahashi, iwai, and tanaka 1982). | we determined almost complete 16s ribosomal dna sequences for 12 actinomycete strains which were either previously classified as kitasatospora strains or defined as streptomyces strains but shown to contain major amounts of meso-diaminopimelic acid in their whole-cell hydrolysates. these sequences were subjected to phylogenetic analyses together with the sequences of 34 streptomyces species. phylogenetic trees were reconstructed by using both neighbor-joining and maximum-parsimony methods. the k ... | 1997 | 9336904 |
| kitasatospora cheerisanensis sp. nov., a new species of the genus kitasatospora that produces an antifungal agent. | an actinomycete, strain yc75t, which produced bafilomycin-like antifungal compounds, was identified as a member of the genus kitasatospora on the basis of morphological and chemotaxonomic characteristics. the strain produced the aerial and fragmenting vegetative mycelia consisting of straight chains of 20 or more smooth-surfaced spores. submerged spores were formed in tryptic soy broth. no soluble pigments were formed. whole-cell hydrolysates contained glucose and mannose, but not galactose. the ... | 1999 | 10319499 |
| taxonomic study and morphological differentiation of an actinomycete genus, kitasatospora. | the history and taxonomic criteria of the actinomycete genus, kitasatospora, are described. the reasons for a change of the position of the genus in the classification system are given and a restriction length polymorphism (rflp) method for rapid identification is described. although the bacterial genus, kitasatospora, resembles the fungal one, streptomyces, in its morphology, it is clearly different in its cell-wall composition which includes ll- and meso-dap, glycine and galactose. following a ... | 1999 | 10467659 |
| [taxonomic studies of two streptomycete strains as 4.693 and as 4.702]. | the "setae group" of the genus streptomyces was originally the genus kitasatosporia (omura, 1982). on the basis of 16s rrna analysis, wellington(1992) proposed to reduce kitasatosporia to synonymy with streptomyces as a species group--"setae group". two streptomyces strains, as 4.693 and as 4.702(deposited in ccccm) were examined by using morphological, chemical and nucleic acid ribtyping methods. it was revealed that these two strains have similarity to the type strain streptomyces setae jcm330 ... | 1997 | 11189356 |
| description of two novel species of the genus kitasatospora omura et al. 1982, kitasatospora cineracea sp. nov. and kitasatospora niigatensis sp. nov. | five actinomycete strains, sk-3255t, sk-3406t, sk-3412, sk-3421 and om-5023, were isolated using a novobiocin-containing agar medium from soil samples. these strains produced long spore chains on aerial mycelium and contained ll- and meso-diaminopimelic acids (daps) in the cell wall. on the basis of morphological and chemotaxonomic characteristics and phylogenetic analysis, these five strains were classified into the genus kitasatospora. dna-dna hybridization and comparison of physiological char ... | 2001 | 11594607 |
| purification and some properties of an inhibitor for a yeast pleiotropic drug resistant pump from kitasatospora sp. e-420. | a strain producing an inhibitor for pleiotropic drug resistant 5 (pdr5) was isolated using our original screening system in yeast. the strain was classified and named as kitasatospora sp. e-420. the purified inhibitor (molecular weight = 1,193 by fab-ms) inhibited a pdr5-mediated efflux of cycloheximide and cerulenin. the intracellular accumulation of a fluorescent dye, rhodamine 123, by the inhibitor was also confirmed. some physicochemical data suggested that the pdr5-specific inhibitor was di ... | 2001 | 11758940 |
| tyropeptins a and b, new proteasome inhibitors produced by kitasatospora sp. mk993-df2. ii. structure determination and synthesis. | the structures of tyropeptins a and b, new proteasome inhibitors produced by kitasatospora sp. mk993-df2, were determined by analysis of various nmr experiments. the 1h and 13c nmr of tyropeptins were complicated due to the presence of an aldehyde group. therefore, tyropeptins were converted to their alcohols by sodium borohydride. these alcohol derivatives gave assignable nmr spectra. the stereochemistry of tyropeptins were determined by analysis of acid hydrolysis products from tyropeptins, an ... | 2001 | 11858653 |
| tyropeptins a and b, new proteasome inhibitors produced by kitasatospora sp. mk993-df2. i. taxonomy, isolation, physico-chemical properties and biological activities. | tyropeptins a and b, new proteasome inhibitors, were isolated from the culture broth of kitasatospora sp. mk993-df2. they were purified using ethyl acetate extraction, silica gel column chromatography, sephadex lh-20 column chromatography and hplc. tyropeptin a inhibited the chymotrypsin-like (cht-l) and trypsin-like (t-l) activities of 20s proteasome with ic50 values of 0.1 microg/ml and 1.5 microg/ml respectively, but did not inhibit the peptidylglutamyl-peptide hydrolyzing (pgph) activity of ... | 2001 | 11858671 |
| inhibition of proteasome activity by tyropeptin a in pc12 cells. | tyropeptin a, a potent proteasome inhibitor not reported before, was produced by kitasatospora sp. mk993-df2. in this study, we investigated the effects of tyropeptin a on proteasome activity in pc12 cells. tyropeptin a inhibited the intracellular proteasome activity in a dose-dependent way and seemed to cause neurite outgrowth. as expected, ubiquitinated proteins that should be substrates for the proteasome accumulated in cells treated with tyropeptin a. hence, it appears that tyropeptin a can ... | 2002 | 12450145 |
| streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype i and emendation of the family streptomycetaceae (waksman and henrici (1943)al) emend. rainey et al. 1997. | the taxonomic position of acidophilic actinomycetes selectively isolated from acidic soils and litter was examined using a polyphasic approach. the distinct 16s rdna phyletic branch formed by representative strains was equated with related monophyletic clades that corresponded to the genera kitasatospora and streptomyces. the acidophilic isolates also exhibited a distinctive ph profile, a unique 16s rdna signature, and contained major amounts of ll-diaminopimelic acid, galactose and rhamnose in ... | 2003 | 12785304 |
| isolation of new actinomycete strains for the screening of new bioactive compounds. | in order to facilitate the discovery of novel bioactive compounds from microorganisms, various techniques for isolation of new actinomycete strains have been attempted. studies of the vertical distribution of actinomycetes in soil, isolation of actinomycetes from desert soils or fallen leaves, selective isolation of kitasatospora strains using novobiocin or actinoplanes strains using the chemotactic method, and the use of gellan gum as a solidifying agent were carried out. we discovered 9 novel ... | 2003 | 12949697 |
| kitasatospora putterlickiae sp. nov., isolated from rhizosphere soil, transfer of streptomyces kifunensis to the genus kitasatospora as kitasatospora kifunensis comb. nov., and emended description of streptomyces aureofaciens duggar 1948. | a polyphasic study was undertaken to establish the taxonomic position of a rhizosphere isolate that had been assigned provisionally to the genus kitasatospora. the organism, isolate f18-98(t), was found to have chemical and morphological properties that were consistent with its classification as a kitasatospora strain. direct 16s rdna sequence data confirmed the taxonomic position of the strain, following the generation of phylogenetic trees by using three treeing algorithms. the organism formed ... | 2003 | 14657141 |
| new cytotoxic bafilomycin c1-amide produced by kitasatospora cheerisanensis. | | 2003 | 14700279 |
| intergeneric conjugal transfer of plasmid dna from escherichia coli to kitasatospora setae, a bafilomycin b1 producer. | an effective transformation procedure for kitasatospora setae was established based on transconjugation from escherichia coli et12567 (puz8002) using a phi c31-derived integration vector, pset152, containing orit and attp fragments. while no transconjugation was observed under the standard transconjugation conditions for streptomyces species, sufficient transconjugation (>1 x 10(-6)) was achieved on isp4 medium containing 30 mm mgcl(2) using a 25- to 125-fold excess of e. coli donor cells. in ad ... | 2004 | 14767637 |
| analysis of the endophytic actinobacterial population in the roots of wheat (triticum aestivum l.) by terminal restriction fragment length polymorphism and sequencing of 16s rrna clones. | the endophytic actinobacterial population in the roots of wheat grown in three different soils obtained from the southeast part of south australia was investigated by terminal restriction fragment length polymorphism (t-rflp) analysis of the amplified 16s rrna genes. a new, validated approach was applied to the t-rflp analysis in order to estimate, to the genus level, the actinobacterial population that was identified. actinobacterium-biased primers were used together with three restriction enzy ... | 2004 | 15006805 |
| phylogenetic analysis of the genera streptomyces and kitasatospora based on partial rna polymerase beta-subunit gene (rpob) sequences. | the rna polymerase beta-subunit genes (rpob) of 67 streptomyces strains, representing 57 species, five kitasatospora strains and micromonospora echinospora kctc 9549 were partially sequenced using a pair of rpob pcr primers. among the streptomycetes, 99.7-100 % similarity within the same species and 90.2-99.3 % similarity at the interspecific level were observed by analysis of the determined rpob sequences. the topology of the phylogenetic tree based on rpob sequences was similar to that of 16s ... | 2004 | 15023980 |
| cloning and functional analysis by gene disruption of a gene encoding a gamma-butyrolactone autoregulator receptor from kitasatospora setae. | gamma-butyrolactone autoregulator receptors of the genus streptomyces have a common activity as dna-binding transcriptional repressors, controlling secondary metabolism and/or morphological differentiation. a gene encoding a gamma-butyrolactone autoregulator receptor was cloned from a bafilomycin b1 producer, kitasatospora setae, for the first time from a non-streptomyces genus of actinomycetes, and its function was evaluated by in vitro and in vivo analyses. the gene fragment was initially clon ... | 2004 | 15150228 |
| chitin degrading potential of bacteria from extreme and moderate environment. | five hundred chitin-degrading bacteria were isolated from 20 different locations. high percentage of potent chitin-degraders was obtained from polluted regions. potent chitin-degrading bacteria were selected by primary and secondary screening. among the selected isolates, 78% were represented by the genus streptomyces. majority of the isolates had good chitinolysis relative to the growth although isolates with better growth were also seen. such isolates are important for the production of scp fr ... | 2003 | 15267156 |
| streptacidiphilus jiangxiensis sp. nov., a novel actinomycete isolated from acidic rhizosphere soil in china. | the taxonomic position of three acidophilic actinomycetes isolated from acidic rhizosphere soil was established using a polyphasic approach. the morphological and chemical properties of the isolates were found to be consistent with their assignment to the genus streptacidiphilus. almost complete 16s rrna gene sequences determined for the strains were aligned with corresponding sequences of representatives of the genera kitasatospora, streptacidiphilus and streptomyces and phylogenetic trees infe ... | 2004 | 15280649 |
| five novel kitasatospora species from soil: kitasatospora arboriphila sp. nov., k. gansuensis sp. nov., k. nipponensis sp. nov., k. paranensis sp. nov. and k. terrestris sp. nov. | a polyphasic study was carried out to establish the taxonomic positions of six strains isolated from diverse soil samples and provisionally assigned to the genus kitasatospora. the isolates were found to have chemical and morphological properties consistent with their classification as kitasatospora strains. direct 16s rrna gene sequence data confirmed the taxonomic position of the strains following the generation of phylogenetic trees by using three tree-making algorithms. five of the isolates ... | 2004 | 15545445 |
| kitasatospora viridis sp. nov., a novel actinomycete from soil. | the taxonomic position of a rhizosphere isolate, strain 52108a(t), was determined using a polyphasic approach. the strain was found to have chemical and morphological properties consistent with its assignment to the genus kitasatospora. an almost complete 16s rrna gene sequence determined for the strain was aligned with corresponding sequences of representatives of the genus kitasatospora and related taxa using three tree-making algorithms. the organism formed a distinct phyletic line within the ... | 2005 | 15774648 |
| structure-based design of derivatives of tyropeptin a as the potent and selective inhibitors of mammalian 20s proteasome. | tyropeptin a, a new potent proteasome inhibitor, was produced by kitasatospora sp. mk993-df2. to enhance the inhibitory potency of tyropeptin a, we constructed the structural model of tyropeptin a bound to the site responsible for the chymotrypsin-like activity of mammalian 20s proteasome. based on these modeling experiments, we designed and synthesized several derivatives of tyropeptin a. among them, the most potent compound, tp-104, exhibited a 20-fold enhancement in its inhibitory potency com ... | 2005 | 15780623 |
| new antibiotic sch 725424 and its dehydration product sch 725428 from kitasatospora sp. | a new microbial metabolite sch 725424 (1) was isolated from the culture of kitasatospora sp. the structure elucidation of 1 was accomplished based on nmr spectroscopic analyses as well as extensive structure elucidation of its dehydration product sch 725428 (2). compound 1 showed inhibitory activity against staphylococcus aureus with mic values 1-2 microg/ml, and also displayed weak antifungal activity against saccharomyces cerevisiae (pm503) with an mic 32 microg/ml. | 2005 | 15895527 |
| grouping of streptomycetes using 16s-its rflp fingerprinting. | a total of 463 streptomyces and kitasatospora type strains were screened using 16s-its rflp fingerprinting (combined restriction digest using enzymes bstui and haeiii). in total, 59 clusters could be delineated, each comprising multiple strains with nearly identical patterns. good correlation was found in general with phylogeny, as revealed by 16s rdna sequencing. most strains assigned to a particular 16s-its rflp cluster were classified into the corresponding 16s sequencing cluster whether a 16 ... | 2005 | 15950131 |
| design and evaluation of an oligonucleotide-microarray for the detection of different species of the genus kitasatospora. | an oligonucleotide-microarray method was developed for the detection of kitasatospora species in soil samples. the 16s-23s rdna internal transcribed spacer (its) sequence of these antibiotics-producing actinomycetes was applied to design short oligonucleotide probes. two different 26-mers were synthesized, specific to each species used. additionally, four oligonucleotide probes were designed to evaluate the system. the oligonucleotides were spotted onto slides of the arraytube microarray system ... | 2006 | 16099060 |
| synthesis and activity of tyropeptin a derivatives as potent and selective inhibitors of mammalian 20s proteasome. | tyropeptin a, a potent proteasome inhibitor, was isolated from the culture broth of kitasatospora sp. mk993-df2. we synthesized the derivatives of tyropeptin a to enhance its inhibitory potency. among the synthesized derivatives, the most potent compound, tp-104, exhibited a 20-fold inhibitory potency enhancement for chymotrypsin-like activity of 20s proteasome compared to tyropeptin a. additionally, tp-110 specifically inhibited the chymotrypsin-like activity, but did not inhibit the post-gluta ... | 2005 | 16195592 |
| kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in india. | polyphasic characterization of an actinomycete strain vt-36(t) isolated from a sugar-cane field soil sample collected in punjab state, india, revealed that the strain belongs to the genus kitasatospora. the strain's chemotaxonomic characters and g+c content of dna (76.5 mol%) were typical of members of the genus. analysis of the 16s rrna gene sequence supported the generic affiliation of the strain and showed that its closest phylogenetic relative was kitasatospora putterlickiae f18-98t (= dsm 4 ... | 2006 | 16514020 |
| streptacidiphilus oryzae sp. nov., an actinomycete isolated from rice-field soil in thailand. | the taxonomic position of ten acidophilic actinomycetes isolated from an acidic rice-field soil was established using a polyphasic approach. 16s rrna gene sequences determined for the isolates were aligned with corresponding sequences of representatives of the genera kitasatospora, streptacidiphilus and streptomyces and phylogenetic trees were inferred using four tree-making algorithms. the isolates had identical sequences and formed a distinct branch at the periphery of the streptacidiphilus 16 ... | 2006 | 16738101 |
| community structure of actively growing bacterial populations in plant pathogen suppressive soil. | the bacterial community in soil was screened by using various molecular approaches for bacterial populations that were activated upon addition of different supplements. plasmodiophora brassicae spores, chitin, sodium acetate, and cabbage plants were added to activate specific bacterial populations as an aid in screening for novel antagonists to plant pathogens. dna from growing bacteria was specifically extracted from the soil by bromodeoxyuridine immunocapture. the captured dna was fingerprinte ... | 2007 | 16944345 |
| production of epsilon-poly-l-lysine by newly isolated kitasatospora sp. pl6-3. | a novel epsilon-poly-l-lysine (epsilon-pl)-producing strain pl6-3 was isolated from soil, and was identified as a strain of kitasatospora sp. this is the first detailed report of production of epsilon-pl by a strain in the genera of kitasatospora. by controlling the culture ph at 4.0, the yield of epsilon-pl from pl6-3 reached 13.9 g/l after 120 h of cultivation in fed-batch fermentation. the morphological characteristics of kitasatospora sp. pl6-3 in culture broth were different from those repo ... | 2006 | 17161019 |
| talosins a and b: new isoflavonol glycosides with potent antifungal activity from kitasatospora kifunensis mjm341. i. taxonomy, fermentation, isolation, and biological activities. | in our screening program for new antifungal agents from microbial secondary metabolites, we isolated two new isoflavonol glycosides, genistein 7-alpha-l-6-deoxy-talopyranoside (talosin a) and genistein 4',7-di-alpha-l-6-deoxy-talopyranoside (talosin b), from the culture broth of kitasatospora kifunensis mjm341. the talosins exhibited strong antifungal activity against candida albicans, aspergillus niger and cryptococcus neoformans with minimal inhibitory concentrations (mic) in the range of 3- 1 ... | 2006 | 17191678 |
| talosins a and b: new isoflavonol glycosides with potent antifungal activity from kitasatospora kifunensis mjm341. ii. physicochemical properties and structure determination. | in our screening program for new antifungal agents from microbial secondary metabolites, two new isoflavonol glycosides, with potent antifungal activity, talosins a and b, were isolated from the culture broth of kitasatospora kifunensis mjm341. talosins a and b were determined to be genistein 7-alpha-l-6-deoxy-talopyranoside and genistein 4',7-di-alpha-l-6-deoxy-talopyranoside, respectively, by spectroscopic studies. they are the first flavonoid glycosides incorporating 6-deoxy-talose as a sugar ... | 2006 | 17191679 |
| differentiation of streptomyces spp. which cause potato scab disease on the basis of partial rpob gene sequences. | in a previous study, we found that the phylogenetic analysis of partial rpob sequences can be used effectively to phylogenetically differentiate streptomyces spp. [b.j. kim, c.j. kim, j. chun, y.h. koh, s.h. lee, j.w. hyun, c.y. cha, y.h. kook, phylogenetic analysis of the genera streptomyces and kitasatospora based on partial rna polymerase beta-subunit gene (rpob) sequences, int. j. syst. evol. microbiol. 54 (2004) 593-598]. in the present study, we analyzed the partial rpob gene sequences of ... | 2007 | 17320334 |
| isolation and characterization of four gram-positive nickel-tolerant microorganisms from contaminated sediments. | microbial communities from riparian sediments contaminated with high levels of ni and u were examined for metal-tolerant microorganisms. isolation of four aerobic ni-tolerant, gram-positive heterotrophic bacteria indicated selection pressure from ni. these isolates were identified as arthrobacter oxydans nr-1, streptomyces galbus nr-2, streptomyces aureofaciens nr-3, and kitasatospora cystarginea nr-4 based on partial 16s rdna sequences. a functional gene microarray containing gene probes for fu ... | 2007 | 17404787 |
| a new proteasome inhibitor, tp-110, induces apoptosis in human prostate cancer pc-3 cells. | proteasome inhibitors are useful in the treatment of cancer. recently, we found a new proteasome inhibitor, tp-110, derived from tyropeptin a produced by kitasatospora sp. here we report that tp-110 induces apoptosis in human prostate cancer pc-3 cells. tp-110 showed strong cytotoxicity to pc-3 cells (ic(50)=0.05 mum). it increased the number of cells in the g(2)-m phase and increased the accumulated amounts of the p21 and p27 proteins, which are negative regulators of cell cycle progression. fu ... | 2007 | 17420589 |
| comparative genomics of streptomyces avermitilis, streptomyces cattleya, streptomyces maritimus and kitasatospora aureofaciens using a streptomyces coelicolor microarray system. | dna/dna microarray hybridization was used to compare the genome content of streptomyces avermitilis, streptomyces cattleya, streptomyces maritimus and kitasatospora aureofaciens with that of streptomyces coelicolor a3(2). the array data showed an about 93% agreement with the genome sequence data available for s. avermitilis and also showed a number of trends in the genome structure for streptomyces and closely related kitasatospora. a core central region was well conserved, which might be predic ... | 2008 | 17588127 |
| antineoplastic agents. 560. isolation and structure of kitastatin 1 from an alaskan kitasatospora sp. | by utilizing a bioassay-guided separation (p388 lymphocytic leukemia and a panel of human cancer cell lines) of fermentation broths from a kitasatospora sp. collected from a tundra soil sample taken at the shore of the beaufort sea, we have isolated three powerful (gi50 to 0.0006 microg/ml) cancer cell growth inhibitors (1-3) and determined their structures to be closely related cyclodepsipeptides. from 380 l fermentations of kitasatospora sp. were obtained 2.6 mg of a new cyclodepsipeptide desi ... | 2007 | 17608530 |
| electrical dna-chip-based identification of different species of the genus kitasatospora. | the identification of different kitasatospora strains has been shown with a dna-chip based on an electrical readout scheme. the 16s-23s rdna internal transcribed spacer region of these actinomycetes was used for identification. two different capture probes per strain were immobilized on the chip. the capture probes were spotted on a dna-chip with electrode structures for an electrical dna detection. a biotinylated pcr product of the 16s-23s rdna region was incubated on the chips and bound to its ... | 2008 | 17994235 |
| application of conjugation using phic31 att/int system for actinoplanes teichomyceticus, a producer of teicoplanin. | actinoplanes teichomyceticus produces teicoplanin, which is a glycopeptide antibiotic for gram-positive pathogenic bacteria and methicillin-resistant staphylococcus aureus (mrsa). for a molecular genetic study of a. teichomyceticus, an effective transformation method using the conjugal transfer of dna from e. coli to spores of a. teichomyceticus was established for the first time, based on the bacteriophage varphic31 att/int system, in the genus of actinoplanes. the high frequency of transconjug ... | 2008 | 18317703 |
| in vitro antagonism of an actinobacterial kitasatospora isolate against the plant pathogen phytophthora citricola as elucidated with ultrahigh resolution mass spectrometry. | many soil microorganisms antagonistic to soil borne plant pathogens are well known for their ability to control diseases in situ. a variety of substances, like lytic enzymes, siderophores and antibiotics, produced by these organisms have the potential to protect roots against pathogens. understanding the ecology and a functional assessment of antagonistic microbial communities in soil requires in-depth knowledge of the mechanisms involved in these interactions, a challenging task in complex syst ... | 2008 | 18588924 |
| direct detection of kitasatospora species with a chaperone oligonucleotide microarray method lacking pcr amplification. | identification of members of the genus kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. the microarray hybridization usually involves pcr amplification of the target dna. since pcr might lead to biased amplification, a diagnostic kitasatospora microarray technique was improved by a protocol lacking pcr amplification prior to hybridization. the described advanced hybridization method used chaperone oligonucle ... | 2008 | 18720491 |
| kitasatospora saccharophila sp. nov. and kitasatospora kazusanensis sp. nov., isolated from soil and transfer of streptomyces atroaurantiacus to the genus kitasatospora as kitasatospora atroaurantiaca comb. nov. | a polyphasic study was undertaken to establish the taxonomic positions of two isolates, sk15(t) and sk60(t), from soil samples that were found to have morphological and chemical properties consistent with kitasatospora strains. phylogenetic analysis based on the 16s rrna gene sequence revealed that strains sk15(t) and sk60(t) form novel evolutionary lineages within the radiation of the genus kitasatospora and share the highest 16s rrna gene sequences similarities with their closest relatives, ki ... | 2009 | 19282629 |
| bioweathering and biotransformation of granitic rock minerals by actinomycetes. | actinomycetes inhabiting granitic rocks at st. katherine, egypt were investigated for their bioweathering potential. actinomycete counts ranged between 174 and 360 colony forming units per gram. counts were positively correlated to rock porosity (r = 0.65) and negatively correlated to rock salinity (r = -0.56). sixty-six actinomycete isolates originating from rocks could be assigned into eight genera, with a high frequency of nocardioides and streptomyces. organic acids were produced by 97% of t ... | 2009 | 19590809 |
| isolation and identification of mucinolytic actinomycetes. | biochemical and physiological tests, and 16s rrna gene sequences, were used to classify nine actinomycete strains isolated from soil and sand samples in thailand. these strains were isolated based on their ability to readily degrade mucin glycoproteins. a turbidometric based mucinolytic assay was developed to confirm this. in addition all strains showed significant production of proteases. phylogenetic analysis of the strains revealed that from the nine isolated actinomycete strains eight were c ... | 2010 | 19997863 |
| epsilon-poly-l-lysine production by immobilized cells of kitasatospora sp. my 5-36 in repeated fed-batch cultures. | the production of epsilon-pl by kitasatospora sp. my 5-36 through entrapment or adsorption on bagasse, synthetic sponge, macroporous silica gel, and loofah sponge was investigated in shake flask cultures, and immobilization on loofah sponge gave the highest epsilon-pl production. repeated fed-batch cultures for epsilon-pl production were also carried out in a stirred bioreactor and final epsilon-pl concentrations and productivity of 34.11 g l(-1) and 9.34 g l(-1)d(-1), respectively were achieved ... | 2010 | 20335030 |
| isolation of new fuzanins, carbamate-containing natural products, from kitasatospora sp. ifm10917. | | 2010 | 20531434 |
| staphylococcus aureus promoter-lux reporters for drug discovery. | we describe a collection of antibiotic-activated staphylococcus aureus promoter-lux reporter strains that can be used to discriminate among antibiotic classes on the basis of their light production response profile. we screened over 400 culture supernatants from previously uncharacterized actinomycetes from soil for the production of aminocoumarin-type compounds and dna-damaging agents. novobiocin production was determined in three isolates of streptomyces, and streptonigrin, a dna-damaging agen ... | 2010 | 20606700 |
| cloning and in vitro characterization of dtdp-6-deoxy-l-talose biosynthetic genes from kitasatospora kifunensis featuring the dtdp-6-deoxy-l-lyxo-4-hexulose reductase that synthesizes dtdp-6-deoxy-l-talose. | kitasatospora kifunensis, the talosin producer, was used as a source for the dtdp-6-deoxy-l-talose (dtdp-6dtal) biosynthetic gene cluster, serving as a template for four recombinant proteins of rmla(kkf), rmlb(kkf), rmlc(kkf), and tal, which complete the biosynthesis of dtdp-6dtal from dttp, alpha-d-glucose-1-phosphate, and nad(p)h. the identity of dtdp-6dtal was validated using (1)h and (13)c nmr spectroscopy. k. kifunensistal and tll, the known dtdp-6dtal synthase gene of actinobacillus actino ... | 2010 | 20667525 |
| genome sequence of kitasatospora setae nbrc 14216t: an evolutionary snapshot of the family streptomycetaceae. | kitasatospora setae nbrc 14216(t) (=km-6054(t)) is known to produce setamycin (bafilomycin b1) possessing antitrichomonal activity. the genus kitasatospora is morphologically similar to the genus streptomyces, although they are distinguishable from each other on the basis of cell wall composition and the 16s rdna sequence. we have determined the complete genome sequence of k. setae nbrc 14216(t) as the first streptomycetaceae genome other than streptomyces. the genome is a single linear chromoso ... | 2010 | 21059706 |
| iodide accumulation by aerobic bacteria isolated from subsurface sediments of a 129i-contaminated aquifer at the savannah river site, south carolina. | (129)i is of major concern because of its mobility in the environment, excessive inventory, toxicity (it accumulates in the thyroid), and long half-life (~16 million years). the aim of this study was to determine if bacteria from a (129)i-contaminated oxic aquifer at the f area of the u.s. department of energy's savannah river site, sc, could accumulate iodide at environmentally relevant concentrations (0.1 µm i(-)). iodide accumulation capability was found in 3 out of 136 aerobic bacterial stra ... | 2011 | 21278282 |
| chromosome diversity and similarity within the actinomycetales. | many chromosomes from actinomycetales, an order within the actinobacteria, have been sequenced over the last 10 years and the pace is increasing. this group of gram-positive and high g+c% bacteria is economically and medically important. however, this group of organisms also is just about the only order in the kingdom bacteria to have a relatively high proportion of linear chromosomes. chromosome topology varies within the order according to the genera. streptomyces, kitasatospora and rhodococcu ... | 2011 | 21320158 |
| in vivo metabolism of talosin a, new isoflavonol glycoside from kitasatospora kifunensis, in rats. | the isoflavonol glycoside talosin a, genistein (gt)-7-α-l-6-deoxy talopyranose (gt-tal), was first isolated from the culture broth of kitasatospora kifunensis mjm341. the aim of the present study was to evaluate the oral absorption and metabolism of the newly isolated isoflavonol glycoside, gt-tal compared to genistin (gt-7-o-β-d-glucopyranoside; gt-glu). free gt-glu and gt-tal could not be detected prior to enzymatic hydrolysis of the corresponding conjugates in rat plasma. following oral admin ... | 2011 | 21586869 |
| In vitro phosphinate methylation by PhpK from Kitasatospora phosalacinea. | Radical S-adenosyl-L-methionine, cobalamin-dependent methyltransferases have been proposed to catalyze the methylations of unreactive carbon or phosphorus atoms in antibiotic biosynthetic pathways. To date, none of these enzymes has been purified or shown to be active in vitro. Here we demonstrate the activity of the P-methyltransferase enzyme, PhpK, from the phosalacine producer Kitasatospora phosalacinea. PhpK catalyzes the transfer of a methyl group from methylcobalamin to 2-acetylamino-4-hyd ... | 2011 | 21950770 |