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outbreak of pseudoinfection with tsukamurella paurometabolum traced to laboratory contamination: efficacy of joint epidemiological and laboratory investigation.from january 1988 to may 1989, one hospital in south carolina reported 12 isolates of tsukamurella paurometabolum from 10 patients. there were no common risk factors among the patients. case-control studies revealed that the positive specimens were significantly more likely to have been processed in the tb/fungal room, to have been tissue samples, and to have been handled by one technician. typing on the basis of biochemical, antimicrobial resistance, southern blot, and ribotype profiles showed ...19921318084
pseudoinfection due to tsukamurella paurometabolum. 19921445990
induction of interferons (ifns) and tumor necrosis factor (tnf) in mice by a novel glycolipid trehalose 2,3,6'-trimycolate from rhodococcus aurantiacus (gordona aurantiaca).the immunomodifying activity of a novel mycoloyl glycolipid, trehalose 2,3,6'-trimycolate (gagm), from a unique psychrophilic acid-fast bacterium, rhodococcus aurantiacus, was examined. icr mice were primed intravenously (i.v.) or intraperitoneally (i.p.) with liposomes containing gagm (300 micrograms/mouse), and were administered lps dissolved in saline (25 micrograms/mouse, i.v.) 2 weeks later. two hours after injection of lps, interferons (ifns) and tumor necrosis factor (tnf) were induced si ...19901699114
[protective effect of glycolipid containing mycolic acid derived from gordona aurantiaca against yersinia enterocolitica]. 19883137281
severe progressive subcutaneous abscesses and necrotizing tenosynovitis caused by rhodococcus aurantiacus.a case of severe progressive subcutaneous abscesses and necrotizing tenosynovitis of the right arm of a 30-year-old woman caused by rhodococcus aurantiacus is reported.19883343320
separation and analysis of novel polyunsaturated mycolic acids from a psychrophilic, acid-fast bacterium, gordona aurantiaca.more than 30 molecular species of highly unsaturated mycolic acids, ranging from c60 to c78 and possessing between two and seven double bonds, have been obtained from a new genus of acid-fast bacteria, gordona aurantiaca. they were fully separated and identified as their trimethylsilyl ether derivatives by a combination of silica gel thin-layer chromatography (tlc), argentation thin-layer chromatography and gas chromatography/mass spectrometry (gc/ms). on silica gel thin-layer chromatography two ...19846698005
persistent bacteremia due to tsukamurella paurometabolum in a patient undergoing hemodialysis: case report and review. 19948075284
a cancer patient with central venous catheter-related sepsis caused by tsukamurella paurometabolum (gordona aurantiaca) 19938399886
comparison of 16s ribosomal rna genes in clavibacter michiganensis subspecies with other coryneform bacteria.nearly complete sequences (97-99%) of the 16s rrna genes were determined for type strains of clavibacter michiganensis subsp. michiganensis, clavibacter michiganensis subsp. insidiosus, clavibacter michiganensis subsp. sepedonicus, and clavibacter michiganensis subsp. nebraskensis. the four subspecies had less than 1% dissimilarity in their 16s rrna genes. comparative studies indicated that the c. michiganensis subsp. shared relatively high homology with the 16s rrna gene of clavibacter xyli. fu ...19958590406
cutaneous infection caused by tsukamurella paurometabolum. 19968909862
neuropeptides in the livers of mice during bacterial infections.neuropeptides such as substance p (sp) and vasoactive intestinal peptide (vip) are known to act as immunomodulators. we investigated the induction of sp and vip in the livers of mice infected with listeria monocytogenes or injected with tsukamurella paurometabolum. vip was detected in the livers of mice after l. monocytogenes infection by an immunohistochemical technique and preprovip mrna, which was detected by reverse transcription-polymerase chain reaction (pcr), was induced post infection. h ...19989544784
sensitivity to capreomycin and prothionamide in strains of mycobacterium, nocardia, rhodococcus, and related taxa for taxonomical purposes.sensitivity to capreomycin and to prothionamide was analysed for 150 strains belonging to the genera mycobacterium, nocardia, rhodococcus, and related taxa. the analyses showed, e.g., that strains of mycobacterium chelonei and nocardia brasiliensis were more resistant to capreomycin than the other strains tested and that m. farcinogenes differed from m. senegalense concerning sensitivity to this drug. the analysis showed, furthermore, that strains of mycobacterium differ from those of nocardia, ...19836196927
[a new species gordona aurantiaca occurring in sputa of patients with pulmonary disease]. 19715572851
central venous catheter-related bacteremia due to tsukamurella species in the immunocompromised host: a case series and review of the literature.we report 6 cases of bacteremia due to tsukamurella species, all of which were in immunosuppressed patients with indwelling central venous catheters (cvcs). fewer than 20 cases of serious illness due to these gram-positive bacilli have been reported in the medical literature; these cases have mostly been ascribed to the species tsukamurella paurometabola. tsukamurella species are frequently misidentified as rhodococcus or corynebacterium species. we used high-performance liquid chromatography to ...200212228839
meningitis caused by gordona aurantiaca (rhodococcus aurantiacus).in a case of hairy cell leukemia, gordona aurantiaca (rhodococcus aurantiacus) was isolated from cerebrospinal fluid as the pathogen responsible for lethal infection of the central nervous system. the pathogen had been isolated previously from one case of pulmonary infection process only.19854044805
evidence for the pathogenic role of rhodococcus species in pulmonary diseases.three hundred expectorated sputa from patients suffering from chest disorders were examined microscopically and cultured on various media. among other micro-organisms isolated there were four strains of rhodococcus aurantiacus (gordona aurantiaca), one each of rh. erythropolis, rh. pellegrino, rh. rubropertinctus and rh. rhodnii. suspensions in 5% hog gastric mucin were virulent for immuno-suppressed white mice and the pathology of their lungs was compatible with those produced in experimental n ...19892753843
tsukamurella paurometabola lipoglycan, a new lipoarabinomannan variant with pro-inflammatory activity.the genus tsukamurella is a member of the phylogenetic group nocardioform actinomycetes and is closely related to the genus mycobacterium. the mycobacterial cell envelope contains lipoglycans, and of particular interest is lipoarabinomannan, one of the most potent mycobacterial immunomodulatory molecules. we have investigated the presence of lipoglycans in tsukamurella paurometabola and report here the isolation and structural characterization of a new lipoarabinomannan variant, designated tpala ...200415031299
[granuloma formation in mice by liposomes of new glycolipid containing mycolic acids, "trehalose 2,3,6'-trimycolate"].after intravenous administration of liposomes containing a novel glycolipid, trehalose 2,3,6'-trimycolate, isolated from rhodococcus aurantiacus, the granuloma formation was observed distinctively in the lungs, spleen and liver of icr mice. the concentration of the glycolipid in liposomes influenced profoundly on the inducibility for granulomas and the liposomes containing 10 mol% of trehalose 2,3,6'-trimycolate showed the highest activity for the granuloma formation in mice without a significan ...19892732903
[in vitro activation of immune cells by a mycolic acid-containing glycolipid, trehalose-2,3,6'-trimycolate, derived from gordona aurantiaca].a mycolic acid-containing glycolipid, trehalose-2,3,6'-trimycolate (gagm), derived from gordona aurantiaca, an acid-fast bacteria closely related taxonomically to mycobacterium, was investigated for its immune adjuvant activity in vitro. the liposomes containing gagm showed strong mitogenic effects on murine spleen cells at the doses used (25-100 micrograms/ml), but not on t-cell-depleted spleen cells or macrophage-depleted spleen cells. these results suggest that the mitogenic property of lipos ...19892533624
induction of tumoricidal activated macrophages by a liposome-encapsulated glycolipid, trehalose 2,3,6'-trimycolate from gordona aurantiaca.a mycolic acid-containing glycolipid, trehalose 2,3,6'-trimycolate, prepared from a non-pathogenic acid-fast bacterium gordona aurantiaca, was shown to induce strong tumoricidal activity in peritoneal exudate cells by intravenous or intraperitoneal injection of liposome-encapsulated preparations. the mycolic acid derivative containing a high proportion of unsaturated fatty acids rendered macrophages cytotoxic against syngeneic mastocytoma cells in the absence of endotoxin, for over 14 days after ...19902257164
culture and spray-drying of tsukamurella paurometabola c-924: stability of formulated powders.the nematocidal agent, tsukamurella paurometabola c-924, was cultured in a 300 l bioreactor. spray-dried formulations of this microorganism were prepared using sucrose. at an outlet temperature 62 degrees c, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). the stability study of the powders showed that the best storage condition was at 4 degrees c under vacuum. a new method for the calculation of cell death order for bacteria stored at low temperatures was developed. ...200717603774
growth inhibition of tumour cells by a liposome-encapsulated, mycolic acid-containing glycolipid, trehalose 2,3,6'-trimycolate.in vivo growth of syngeneic tumour cells in the peritoneal cavity was strongly inhibited by intraperitoneal injection of a liposome-encapsulated, mycolic acid-containing glycolipid, trehalose 2,3,6'-trimycolate (ttm), derived from a non-pathogenic, acid-fast bacterium. gordona aurantiaca. peritoneal macrophages from mice after this treatment lysed tumour cells in vitro at a low effector/target ratio, and their culture supernatant inhibited tumour cell growth. the supernatant inhibited growth of ...19911769697
tsukamurella paurometabolum: a novel pathogen causing catheter-related bacteremia in patients with cancer.tsukamurella paurometabolum is a weakly acid-fast, pleomorphic gram-positive bacterium found in soil. human infection due to this organism has rarely been described, and there are no published accounts of bacteremia. three cases of bacteremia due to t. paurometabolum and related to long-term use of a central venous catheter in patients with cancer who were receiving chemotherapy are described.19921571430
tsukamurella strandjordae sp. nov., a proposed new species causing sepsis.we have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. this isolate was compared with 14 other strains including reference strains of tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, dna-dna hybridization, and sequencing of the 16s rrna gene. this isolate represents a new tax ...200111283073
movement disorders in encephalitis induced by rhodococcus aurantiacus infection relieved by the administration of l-dopa and anti-t-cell antibodies.mice injected with rhodococcus aurantiacus by the intravenous (i.v.) route show neurological disorders, hemiparesis, vertical headshake and turn-round gait after day 7 postinfection (p.i.). neurological symptoms caused by i.v. inoculation of r. aurantiacus were relieved by treatment with levodopa (l-dopa). r. aurantiacus was isolated from the brain and was found to be completely eliminated at day 7 p. i. focal encephalitis was mainly observed in the brain stem, and t cells could be isolated from ...199910233672
tsukamurella tyrosinosolvens sp. nov.chemotaxonomic and 16s ribosomal dna sequence analyses of four bacterial isolates from blood cultures from patients with cardiac pacemaker implants and sputa of patients with chronic lung infections clearly demonstrated that these bacteria belong to the genus tsukamurella. dna-dna hybridization data, as well as the physiological characteristics of the isolates, indicate that they are closely related and belong to a single species that differs from previously described members of the genus tsukam ...19979226890
[tsukamurella infections. review of the literature apropos of a case].the genus tsukamurella belongs to the family nocardiaceae, and is an environmental saprophyte. the type species is tsukamurella paurometabola. its microbiological identification and differentiation from the other species containing mycolic acids can be difficult. there has been a few cases of human infections reported, usually in patients with special conditions, such as chronic lung pathology, immuno-suppression (leukemia, solid tumors, maybe hiv-infection) or the long-term use of indwelling ca ...19979097849
tsukamurella pulmonis sp. nov.chemotaxonomic and 16s ribosomal dna sequence analyses of an isolate from the sputum of a patient with a mycobacterial lung infection clearly delineated a new species of the genus tsukamurella. this new species can be defined on the basis of genotypic and phenotypic data. the name tsukamurella pulmonis sp. nov. is proposed for this organism; the type strain is immib d-1321t (= dsm 44142t). this isolate shows 44.2 and 36.2% dna relatedness to tsukamurella paurometabola dsm 20162t (t = type strain ...19968934902
reciprocal action of interferon-gamma and interleukin-4 promotes granulomatous inflammation induced by rhodococcus aurantiacus in mice.an intravenous injection of rhodococcus aurantiacus to mice causes granulomatous inflammation dependent on endogenous interferon-gamma (ifn-gamma). the present study examined the role of endogenous interleukin-4 (il-4) on granulomatous inflammation. endogenous il-4 in the spleen extracts was not detected during the phase of granuloma formation by enzyme-linked immunosorbent assay (elisa). however, il-4 protein level was elevated during the phase of granuloma regression. il-4 mrna expression in t ...19968774356
tsukamurella inchonensis sp. nov.chemotaxonomic and genomic 16s ribosomal dna sequence analyses of two isolates obtained from two different clinical materials clearly delineated a new species of the genus tsukamurella. this new species can be identified by its 16s ribosomal dna similarity values, as well as its physiological characteristics. the name tsukamurella inchonensis sp. nov. is proposed for these isolates, which are represented by strain immib d-771t (= dsm 44067t) (t = type strain). this strain exhibits only 45% dna r ...19958590680
sequential involvement of nk cells and cd8+ t cells in granuloma formation of rhodococcus aurantiacus-infected mice.we investigated the effect of in vivo administration of antibodies against t-cell subsets and natural killer (nk) cells on endogenous gamma interferon (ifn-gamma) production and granuloma formation in rhodococcus aurantiacus-infected mice. high titers of endogenous ifn-gamma were detected in the extracts of the livers and spleens during 24 hr of the infection, reaching the peak at 8 hr, and the ifn-gamma production was reduced by in vivo administration of anti-nk 1.1 monoclonal antibody (mab) or ...19958569535
endogenous gamma interferon is essential in granuloma formation induced by glycolipid-containing mycolic acid in mice.the present study examined the role of endogenous gamma interferon (ifn-gamma) in the formation of granulomas in mice which had been given a single intravenous injection of glycolipid-containing mycolic acid (trehalose 2,3,6'-trimycolate) purified from cell walls of rhodococcus aurantiacus (gordona aurantiaca) (gagm) in the form of liposome. the histological status of granuloma formation in the livers, spleens, and lungs of gagm-injected mice was studied at weeks 1 through 5, and the titers of e ...19938514390
a simple chemical test to distinguish mycobacteria from other mycolic-acid-containing actinomycetes.two hundred and fifty-two representatives of the general corynebacterium, gordona, mycobacterium, nocardia, rhodococcus and tsukamurella were degraded by alkaline hydrolysis and their mycolic acids extracted as methyl esters following phase-transfer-catalysed esterification. when the mycolic acid methyl esters were treated with a mixture of acetonitrile and toluene all mycobacterial mycolates formed copious white precipitates whereas all but 5 out of the 106 non-mycobacterial mycolates remained ...19938245845
rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a mycobacterium library.current methods for identifying mycobacteria by high-performance liquid chromatography (hplc) require a visual assessment of the generated chromatographic data, which often involves time-consuming hand calculations and the use of flow charts. our laboratory has developed a personal computer-based file containing patterns of mycolic acids detected in 45 species of mycobacterium, including both slowly and rapidly growing species, as well as tsukamurella paurometabolum and members of the genera cor ...19948195387
permeabilization of mycolic-acid-containing actinomycetes for in situ hybridization with fluorescently labelled oligonucleotide probes.the application of whole-cell hybridization using labelled oligonucleotide probes in microbial systematics and ecology is limited by difficulties in permeabilizing many gram-positive organisms. in this investigation paraformaldehyde treatment, acid methanolysis and acid hydrolysis were evaluated as a means of permeabilizing mycolic-acid-containing actinomycetes prior to hybridization with a fluorescently labelled oligonucleotide probe designed to bind to a conserved sequence of bacterial 16s rrn ...19948000549
phylogenetic analysis of mycolic acid-containing wall-chemotype iv actinomycetes and allied taxa by partial sequencing of ribosomal protein at-l30.the phylogenetic relationships among 30 mycolic acid-containing wall chemotype iv actinomycete strains and 12 strains belonging to allied taxa were examined by determining the amino acid sequences of the ribosomal at-l30 proteins of these organisms. sequencing 20 n-terminal amino acids of at-l30 preparations revealed that the members of the genera containing mycolic acid-containing actinomycetes form two clusters; the first cluster contains the genera nocardia, rhodococcus, gordona, and tsukamur ...19957547284
lung infection caused by gordona aurantiaca (rhodococcus aurantiacus).we report that gordona aurantiaca (rhodococcus aurantiacus) caused a lung infection in humans. the case of a 50-year-old male farmer is shown. this is the first report of infection by this organism.19827153308
role of t cells in granuloma formation induced by rhodococcus aurantiacus is independent of their interferon-gamma production.intravenous injection of rhodococcus aurantiacus into mice causes granulomatous inflammation dependent on endogenous interferon-gamma (ifn-gamma). this study investigated the mechanism of granuloma formation with an adoptive transfer system in ifn-gamma knockout (ifn-gamma(-/-)) mice. ifn-gamma(-/-) mice infected with r. aurantiacus did not develop granulomas, and high titres of endogenous interleukin-10 (il-10) were detected in spleen extracts at 2 weeks after infection. the adoptive transfer o ...200111478672
tsukamurella spumae sp. nov., a novel actinomycete associated with foaming in activated sludge plants.a polyphasic taxonomic study was undertaken to establish the taxonomic position of six representative strains isolated from activated sewage sludge foam. the organisms were found to have chemical and morphological properties consistent with their assignment to the genus tsukamurella. dna:dna relatedness studies showed that five out of the six isolates formed a distinct genomic species, the remaining strain was most closely associated with this taxon. the five isolates had a unique phenotypic pro ...200314529179
[an immune adjuvant activity of mycolic acid-containing glycolipid, trehalose-2,3,6'-trimycolate, derived from gordona aurantiaca].a mycolic acid-containing glycolipid, trehalose-2,3,6'-trimycolate (gagm), derived from gordona aurantiaca, an acid-fast bacterium closely related taxonomically to mycobacterium, was investigated for its immune adjuvant activity on cell-mediated responses in the mouse. i.v. injection of liposomes containing gagm enhanced the generation of cytotoxic t-lymphocyte (ctl) against syngeneic and allogeneic tumor cells. in addition, the injection of gagm augmented the natural killer (nk) activity and th ...19892788754
polyphasic taxonomic study of the genera gordona and tsukamurella including the description of tsukamurella wratislaviensis sp. nov.representatives of the genera gordona and tsukamurella together with related actinomycetes were the subject of chemotaxonomic, numerical taxonomic and dna homology studies. in the numerical analysis the organisms were examined for 116 unit characters and the data sorted using the simple matching, jaccard and pattern coefficients; clustering was achieved using the unweighted pair group method with arithmetic averages algorithm. the numerical groupings were not affected by the statistics used or b ...19911930572
complete genome sequence of tsukamurella paurometabola type strain (no. 33).tsukamurella paurometabola corrig. (steinhaus 1941) collins et al. 1988 is the type species of the genus tsukamurella, which is the type genus to the family tsukamurellaceae. the species is not only of interest because of its isolated phylogenetic location, but also because it is a human opportunistic pathogen with some strains of the species reported to cause lung infection, lethal meningitis, and necrotizing tenosynovitis. this is the first completed genome sequence of a member of the genus ts ...201121886861
up-regulation of granulomatous inflammation in interleukin-6 knockout mice infected with rhodococcus aurantiacus.after intravenous injection of rhodococcus aurantiacus normal mice develop non-necrotic granulomas, the formation of which is dependent on endogenous interferon-gamma (ifn-gamma). in the early phase of r. aurantiacus infection a high level of endogenous interleukin-6 (il-6) is detected in the spleen extracts, though its importance is unknown. using il-6 knockout (il-6-/-) mice, we studied the role of il-6 in granulomatous inflammation induced by r. aurantiacus. the size of granulomas generated i ...200314632648
structural elucidation of the predominant motifs of the major cell wall arabinogalactan antigens from the borderline species tsukamurella paurometabolum and mycobacterium fallax.tsukamurella paurometabolum and mycobacterium fallax are members of the suprageneric actinomycete group corynebacterineae that possesses a cell wall skeleton composed of a peptidoglycan to which an arabinogalactan is covalently attached. this polysaccharide is further modified by esterification with c60-c80 mycolic acid residues in mycobacteria and t. paurometabolum. however, m. fallax and t. paurometabolum produce polyenoic (up to six double bonds) mycolic acids whereas the most common type of ...200515761023
production of alkaline lipase by corynebacterium paurometabolum, mtcc 6841 isolated from lake naukuchiatal, uttaranchal state, india.a moderately psychrophilic bacterium corynebacterium paurometabolum mtcc 6841 (gram positive, short rod type) producing extracellular alkaline lipase was isolated from lake naukuchiatal, uttaranchal, india. the bacterium was able to grow within a broad range of ph (5-10). soyabean oil and olive oil served as the best carbon sources for lipase production. the bacterium preferred inorganic nitrogenous compounds, nano3 and kno3, over organic nitrogenous compound for its growth. maximum lipase produ ...200616604420
a regulatory effect of the balance between tnf-alpha and il-6 in the granulomatous and inflammatory response to rhodococcus aurantiacus infection in mice.after i.v. inoculation with rhodococcus aurantiacus, wild-type (wt) mice develop nonnecrotic, epithelioid granulomas. because a high level of tnf-alpha is observed during the initial phase postinfection, we examined the extent to which tnf-alpha contributes to granulomatous inflammation using tnf-alpha gene-deficient (tnf-alpha(-/-)) mice. despite a lack of r. aurantiacus proliferation, tnf-alpha(-/-) mice displayed high mortality rates within 5 days postinfection, as well as a high level of il- ...200616785562
mutual modulation between interleukin-10 and interleukin-6 induced by rhodococcus aurantiacus infection in mice.the interaction between interleukin-10 (il-10) and interleukin-6 (il-6) was investigated in the inflammatory response to rhodococcus aurantiacus (r. aurantiacus) infection, in which both cytokines act as anti-inflammatory cytokines. compared with wild-type (wt) counterparts, il-6 gene-deficient (il-6(-)/(-)) mice mounted a more robust production of il-10 and tumor necrosis factor-alpha (tnf-alpha) during the initial phase of infection. administration of anti-il-10 antibody resulted in all the mi ...200818834949
anhydrobiosis quotient: a novel approach to evaluate stability in desiccated bacterial cells.the major objective of this study was the development of a methodology to quantify the anhydrobiotic ability of bacteria and its application to evaluate the stability of desiccated bacterial cells using the biocontrol agent tsukamurella paurometabola c-924 as a model of anhydrobiote.200919291234
dynamic (11)c-methionine pet analysis has an additional value for differentiating malignant tumors from granulomas: an experimental study using small animal pet.we evaluated whether the dynamic profile of l: -(11)c-methionine ((11)c-met) may have an additional value in differentiating malignant tumors from granulomas in experimental rat models by small animal positron emission tomography (pet).201121732106
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