| comparative studies of the strains pa and pn of mycobacterium phlei leading to their reclassification: examination of lipids and dna, biochemical tests and phage typing. | study of lipid and dna, biochemical tests and phage typing performed on the strain pa previously labelled mycobacterium phlei, lead to the conclusion that this strain belongs to the species m. smegmatis. parallel studies performed on strain pn, isolated from a culture of strain pa, as well as dna homology percentage of the two strains, do not support the assumption that strain pn could have resulted from a mutation of strain pa strain pn produces mycolic acids similar to those found in rhodococc ... | 1979 | 539691 |
| deoxyribonucleic acid reassociation in the classification of the 'rhodochrous' complex and allied taxa. | the degree of binding was determined between dna preparations from gordonae and rhodochrous strains and uracil-labelled dna from five reference strains, nocardia asteroides nk20, n. pellegrino pii, gordona bronchialis ti, g. terrae t5 and rhodochrous strain r90. most of the rhodochrous and pellegrino strains fell into one of two genetically homogeneous taxa. the nucleotide sequence homology data also suggested that g. bronchialis, g. rubra, and more equivocally g. terrae, formed distinct species ... | 1976 | 950551 |
| free mycolic acids as criteria in the classification of gordona and the 'rhodochrous' complex. | the methyl esters of free mycolic acids from representative strains of gordona bronchialis, g. rubra, g. terrae and nocardia kirovani each gave, on mass spectroscopy, homologous series of anhydromycolic esters containing from one to four double bonds with the main components of the parent mycolic acids centered on 56, 58, 62 or 64 carbon atoms (total range from c52 to c66). the mycolic acids from the gordona strains, with chain lengths centered around c60, form a group intermediate in size betwe ... | 1976 | 1245838 |
| development of a highly specific diagnostic 23s rdna oligonucleotide probe for mycobacterium leprae. | a 21-mer dna oligonucleotide probe targeting the 23s rrna of mycobacterium leprae was developed and its high specificity demonstrated by dot-blot hybridization. even under relaxed hybridization and washing conditions (20 degrees c below tm) the probe was highly selective in that positive signals were only detected with m. leprae, about half of the slow-growing and one of the fast-growing reference mycobacteria and gordona bronchialis. at more stringent washing temperatures (6 degrees c below tm) ... | 1990 | 1366740 |
| identification and epidemiological typing of clinical and environmental isolates of the genus rhodococcus with use of a digoxigenin-labeled rdna gene probe. | rhodococcus species are ubiquitous in the environment, and several species have been reported to have pathogenic potential for humans. rhodococcus equi, in particular, has been reported to cause infections in patients with aids. however, the identification of rhodococcus species with use of conventional biochemical tests is problematic, and no simple and reproducible method exists for their rapid identification and differentiation. we found that the type strains of the 20 recognized species in t ... | 1992 | 1381620 |
| a cluster of rhodococcus (gordona) bronchialis sternal-wound infections after coronary-artery bypass surgery. | | 1991 | 1984175 |
| distribution and application of mycobactins for the characterization of species within the genus rhodococcus. | representatives of 11 species of rhodococcus were examined for their ability to synthesize mycobactin, a lipid-soluble siderophore, following iron-limited growth on solidified glycerol/asparagine medium. rhodococcus bronchialis, r. terrae and r. rubropertinctus formed mycobactins, whereas the remaining species (r. coprophilus, r. equi, r. erythropolis, r. rhodnii, r. rhodochrous, r. ruber, r. maris and r. luteus) failed to synthesize these compounds even under conditions of strictly iron-limited ... | 1986 | 3734753 |
| [clinical studies on the patients showing gordona bronchialis (a "mycobacterium" rhodochrous-like organism) in their sputum]. | | 1972 | 4674300 |
| reclassification of mycobacterium phlei pn-bb as rhodococcus bronchialis pn-bb. | mycobacterium phlei pn-bb, induced in the parental strain pn by gamma-radiation and ethyl methanesulfonate, should be reclassified as rhodococcus bronchialis pn-bb. the reclassification is based on the results of lipid analysis, dna-dna hybridization and several biochemical tests, performed in comparison with the parental strain pn, earlier reclassified as r. bronchialis, and r, bronchialis (n654). | 1980 | 7469254 |
| comparison of mycobacterium 23s rrna sequences by high-temperature reverse transcription and pcr. | we describe a modified rrna sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus mycobacterium. we combined the sensitivity of the reverse transcriptase pcr for amplifying nanogram amounts of template rrna material with the elevated extension temperatures used for the thermostable dna polymerase from thermus thermophilus. a 70 degrees c reverse transcription extension step permitted improved read-through of highly structured r ... | 1995 | 7547304 |
| dietzia, a new genus including dietzia maris comb. nov., formerly rhodococcus maris. | sequencing of the 16s ribosomal dnas (rdna) of two strains of rhodococcus maris was performed to determine the relationship of this species to other mycolic acid-containing actinomycetes. for this purpose we also determined the 16s rdna sequences for the type species of the genus rhodococcus, rhodococcus rhodochrous, and for mycobacterium chlorophenolicum (formerly rhodococcus chlorophenolicus), rhodococcus erythropolis, gordona bronchialis, and gordona terrae, for which only partial sequence da ... | 1995 | 7857805 |
| permeabilization of mycolic-acid-containing actinomycetes for in situ hybridization with fluorescently labelled oligonucleotide probes. | the application of whole-cell hybridization using labelled oligonucleotide probes in microbial systematics and ecology is limited by difficulties in permeabilizing many gram-positive organisms. in this investigation paraformaldehyde treatment, acid methanolysis and acid hydrolysis were evaluated as a means of permeabilizing mycolic-acid-containing actinomycetes prior to hybridization with a fluorescently labelled oligonucleotide probe designed to bind to a conserved sequence of bacterial 16s rrn ... | 1994 | 8000549 |
| rapid identification of clinically significant species and taxa of aerobic actinomycetes, including actinomadura, gordona, nocardia, rhodococcus, streptomyces, and tsukamurella isolates, by dna amplification and restriction endonuclease analysis. | a previously described pcr-restriction fragment length polymorphism (rflp) identification schema for nocardia that used an amplified 439-bp segment (amplicon) of the 65-kda heat shock protein gene was evaluated for potential use with isolates of all clinically significant aerobic actinomycetes. the study included 28 reference (american type culture collection) strains and 198 clinical isolates belonging to 20 taxonomic groups. of these 198 isolates, 188 could be differentiated by this pcr-rflp m ... | 1997 | 9157134 |
| improvement of lysine production by analog-sensitive and auxotroph mutants of the acetylene-utilizing bacterium gordona bronchialis (rhodococcus bronchialis). | an acetylene utilizing gordona (rhodococcus) bronchialis strain, screened for the production of fine chemicals, was found to be capable of producing small amounts of lysine. attempts to produce amino-acid analog-resistant and/or sensitive mutants and auxotrophs of this strain with increased lysine production were made following uv-irradiation or n-methyl-n'-nitro-n-nitrosoguanidine (mnng) treatment. the bacterium exhibited surprisingly high resistance levels to the aforementioned mutagens which ... | 1997 | 9276926 |
| wax d of mycobacterium tuberculosis induced osteomyelitis accompanied by reactive bone formation in buffalo rats. | a suspension of heat-killed mycobacterium tuberculosis in liquid paraffin has been reported to induce foot swelling accompanied by new bone formation in buffalo (buf) rats, which are low responders to the induction of adjuvant arthritis. in the present study, we found that wax d, a mycobacterial cell wall peptidoglycan fragment-arabinogalactan-mycolic acid complex, was an effective component of this bacterium for the induction of osteomyelitis accompanied by reactive bone formation in buf rats. ... | 1998 | 9879920 |
| bacteremia caused by gordonia bronchialis in a patient with sequestrated lung. | gordonia species have been recognized as pathogens in immunocompromised and immunocompetent patients. we report the first case of bacteremia due to gordonia bronchialis in a diabetic patient with a sequestrated lung. species identification was confirmed with mycolic acid analysis by high-performance liquid chromatography and sequencing of the 16s rrna gene. | 2004 | 15184495 |
| recurrent breast abscess caused by gordonia bronchialis in an immunocompetent patient. | we present the first reported case of a recurrent breast infection caused by gordonia bronchialis. the infection occurred in a 43-year-old immunocompetent female, and species level identification was obtained with 16s rrna sequencing. | 2005 | 15956447 |
| identification of a lipoarabinomannan-like lipoglycan in the actinomycete gordonia bronchialis. | the cell envelopes of actinomycetes contain lipidated macroamphiphiles, of which the most extensively characterised are the lipoarabinomannans of mycobacteria and related bacteria. we have investigated the mycolic acid-containing actinomycete gordonia bronchialis and identified the presence of a lipoarabinomannan-like lipoglycan. the extraction and purification procedures recovered a second amphiphilic fraction with properties suggesting a phosphatidylinositol mannoside, consistent with studies ... | 2006 | 16320036 |
| [isolation and comparative analysis of glycolipid fractions in bifidobacteria]. | a comparative tlc analysis of lipid extracts from bifidobacterium longum b 379 m, b. bifidum 791, and b. adolescentis 94 bim has been performed. it is demonstrated that carbohydrate-containing lipid components were present in the bacteria, which differed in their chromatographic mobility (rf) from similar compounds isolated from actinomycetes stomatococcus mucilaginosus pcm 2415t, nocardiopsis dassonvillei pcm 2492, propionibacterium propionicum pcm 2431, saccharopolyspora hirsuta pcm 2279 (= at ... | 2005 | 16400987 |
| proposal of a method for the genetic transformation of gordonia jacobaea. | gordonia jacobaea is a recently isolated bacterial species with potential industrial application on account of its ability to store large quantities of trans-canthaxanthin. its genetic manipulation is, however, difficult and cumbersome owing to the presence of mycolic acids in the cell wall and, especially, because of current lack of knowledge about its basic genetics. the present work describes a method for the genetic transformation of g. jacobaea. | 2006 | 16478500 |
| gordonia defluvii sp. nov., an actinomycete isolated from activated sludge foam. | three strains of non-motile, gram-positive, filamentous actinomycetes, isolates j4(t), j5 and j59, initially recognized microscopically in activated sludge foam by their distinctive branching patterns, were isolated by micromanipulation. the taxonomic positions of the isolates were determined using a polyphasic approach. almost-complete 16s rrna gene sequences of the isolates were aligned with corresponding sequences of representatives of the suborder corynebacterineae and phylogenetic trees wer ... | 2006 | 17012545 |
| gordonia soli sp. nov., a novel actinomycete isolated from soil. | a soil isolate, strain cc-ab07t, was characterized using a polyphasic approach. this organism had chemotaxonomic and morphological properties consistent with its classification in the genus gordonia. 16s rrna gene sequence analysis showed that the novel strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus gordonia, its closest neighbours being the type strains of gordonia alkanivorans, gordonia amicalis, gordonia bronchialis, gordonia desulfuri ... | 2006 | 17082398 |
| improved outcome of trypanosoma cruzi infection in rats following treatment in early life with suspensions of heat-killed environmental actinomycetales. | the well-established model of chagas' disease in "l" rats was used to evaluate the effects of three injections of heat-killed gordonia bronchialis, rhodococcus coprophilus or saline on trypanosoma cruzi parasitaemia and acute and chronic myocarditis, sequelae of the infection. two vaccinating injections were given prior to challenge with t. cruzi, and the third, immunotherapeutic, injection was given 7 days after challenge. treatment with either actinomycete significantly reduced acute parasitae ... | 2007 | 17368877 |
| gordonia bronchialis bacteremia and pleural infection: case report and review of the literature. | gordonia species are aerobic actinomycetes recently recognized as causing human disease, often in the setting of intravascular catheter-related infections. we describe a case of gordonia bronchialis bacteremia and pleural space infection in the absence of an indwelling intravascular catheter and review the breadth of reported infections with this emerging pathogen. | 2011 | 21270217 |
| maternal immunization with actinomycetales immunomodulators reduces parasitemias in offspring challenged with trypanosoma cruzi. | this article describes the first use of heat-killed, borate-buffered preparations of aerobic actinomycetales to immunize pregnant animals in order to determine the effect on their pregnancy and fertility and the survival coefficients of their offspring. pregnant rats received three injections of gordonia bronchialis, rhodococcus coprophylus or physiological saline and a proportion of their offspring were challenged with live trypanosoma cruzi at the time of weaning. levels of parasitemia and, in ... | 2011 | 21463197 |
| successful immunotherapy of canine flea allergy with injected actinomycetales preparations. | aims: can heat-killed, borate-buffered suspensions of gordonia bronchialis, rhodococcus coprophilus or tsukamurella inchonensis be used to treat canine flea allergy? materials & methods: organisms cultured on sauton's medium into stationary phase were autoclaved in borate-buffered saline and stored at 10 mg wet weight/ml. intradermal injections of 0.1 ml containing 1 mg of bacilli were administered on the first and 20th days of the study. g. bronchialis and r. coprophilus were most effective in ... | 2011 | 21843084 |
| complete genome sequence of gordonia bronchialis type strain (3410). | gordonia bronchialis tsukamura 1971 is the type species of the genus. g. bronchialis is a human-pathogenic organism that has been isolated from a large variety of human tissues. here we describe the features of this organism, together with the complete genome sequence and annotation. this is the first completed genome sequence of the family gordoniaceae. the 5,290,012 bp long genome with its 4,944 protein-coding and 55 rna genes is part of the genomic encyclopedia of bacteria and archaea project ... | 2010 | 21304674 |
| immunotherapy for the prevention of myointimal hyperplasia after experimental balloon injury of the rat carotid artery. | we assessed the effect of novel immunotherapeutic heat-killed bacterial (actinomycetales) preparations on the development of myointimal hyperplasia (mih) in a rat carotid balloon trauma model and the effect on the immune response by measuring the expression of interferon gamma (ifn-gamma; (th1) and interleukin 4 (il-4; th2). there was a significant reduction (p < .001) in intima/media ratios (mean +/- sem) in the rats treated by immunomodulation (0.52 +/- 0.03 gordonia bronchialis, 0.60 +/- 0.03 ... | 2010 | 20498142 |
| characterization of clinical isolates of gordonia species in japanese clinical samples during 1998-2008. | during 1998-2008, there were 31 strains of gordonia species isolated from clinical specimens in our laboratory. our identification of the 31 strains of gordonia species showed that major pathogenic gordonia species in japan were classifiable, respectively into 14 and 13 strains of gordonia sputi and gordonia bronchialis. the four remaining strains were identified as three gordonia species: g. aichiensis (2 strains), and g. terrae (1 strain), and g. otitidis (1 strain). results of drug susceptibi ... | 2009 | 19488833 |