| frequency of mobiluncus spp. in bacterial vaginosis in italy. | eleven strains of mobiluncus spp. were isolated from 35 vaginal secretions of patients with bacterial vaginosis (6 mobiluncus curtisii and 5 mobiluncus mulieris). | 1992 | 1435353 |
| demonstration of heterogeneity among the antigenic proteins of mobiluncus species. | the protein and antigenic profiles of the american type culture collection type strains of mobiluncus species and those of 114 clinical isolates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and immunoblotting with homologous polyvalent antisera. the majority of isolates (82%) possessed characteristic protein profiles and could be identified to the species level by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. the major protein bands ... | 1990 | 1691207 |
| normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta). | the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ... | 1991 | 1803013 |
| characterization of unusual tetracycline-resistant gram-positive bacteria. | tetracycline-resistant tet m-negative isolates of actinomyces viscosus, eubacterium lentum, mobiluncus curtisii, and mobiluncus mulieris were screened with the tet k, tet l, and tet o dna probes. ten (71%) of the resistant mobiluncus strains hybridized with the tet o probe, two of the three e. lentum strains hybridized with the tet k probe, and the a. viscosus isolate hybridized with the tet l probe. | 1991 | 1810204 |
| [the isolation and identification of mobiluncus curtisii and mobiluncus mulieris in genital specimens]. | amongst 1.202 genital samples studied, the microscopic exam revealed in 179 (14.8%) cases flora compatible with bacterial vaginitis. of these 179 samples, mobiluncus sp was observed in 35 (19.6%) using gram staining and in 18 (10.1%) this microorganism was isolated in agar rlk and anaerobic atmosphere. using biochemical characterization m. curtisii species was found more frequently (15 isolations) than m. mulieris (3 isolations). chromatographic characterization permitted the diagnosis of the ge ... | 1991 | 1924920 |
| reservoir of four organisms associated with bacterial vaginosis suggests lack of sexual transmission. | this study consisted of a search for the possible reservoir and mode of spread of the four bacterial vaginosis-associated organisms mobiluncus mulieris, mobiluncus curtisii, mycoplasma hominis, and gardnerella vaginalis. their occurrence in rectal, oral, and pharyngeal specimens from women with and without bacterial vaginosis, their male sexual consorts, four homosexual men, and children (altogether, 374 people) was studied. genital samples were also obtained from all adults. all four organisms ... | 1990 | 2229386 |
| phylogenetic evidence for the relationship between the genera mobiluncus and actinomyces. | partial reverse transcriptase sequencing of 16s rrna from mobiluncus curtisii and m. mulieris clearly indicate that the genus mobiluncus is not a member of the bacteroidaceae but belongs to the order actinomycetales. the highest degree of relationship is found with the genus actinomyces which is supported by the presence of common physiological properties. | 1989 | 2482221 |
| recovery of mobiluncus curtisii subspecies holmesii from mixed non-puerperal breast abscess. | | 1989 | 2497007 |
| mobiluncus species and other anaerobic bacteria in non-puerperal breast abscesses. | samples of 109 breast masses were cultured aerobically and anaerobically for mobiluncus spp. using selective and non-selective media. two of the 103 evaluable samples were sterile and 57 (55%) yielded facultative bacteria only. the remaining 44 (43%) contained anaerobic growth alone or mixed infections. seven (16%) of the 44 samples with anaerobes, six from women and one from a man, yielded mobiluncus spp. six were identified as mobiluncus curtisii and one as mobiluncus mulieris. there were two ... | 1989 | 2512148 |
| characterisation of monoclonal antibodies for detection of mobiluncus spp. in genital specimens. | monoclonal antibodies were raised to the anaerobic curved rods, mobiluncus curtisii subsp. curtisii nctc 11656, m. curtisii subsp. holmesii nctc 11657 and m. mulieris nctc 11658. three antibodies reacted with the two subspecies of m. curtisii and, when used in combination against clinical isolates, showed 100% sensitivity and specificity in immunofluorescence studies. immunoblotting showed that two of these antibodies reacted with an epitope on a protein which had an electrophoretic mobility cor ... | 1989 | 2677381 |
| antigenic and biochemical characteristics of mobiluncus mulieris and mobiluncus curtisii. | twenty-four strains of mobiluncus mulieris and 27 strains of mobiluncus curtisii were tested with respect to 6 different biochemical characteristics: arginin-decarboxylase activity, beta-galactosidase activity, synergistic hemolysis with staphylococcus aureus, hydrolysis of hippurate, migration through soft agar and reduction of nitrate. antigens of the same strains, prepared by ultrasonication, were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotti ... | 1986 | 3090855 |
| [a case of vaginitis from which candida albicans were isolated with anaerobic motile curved rods, mobiluncus curtisii subsp. holmesii]. | | 1987 | 3131461 |
| ultrastructure of the in situ adherence of mobiluncus to vaginal epithelial cells. | from patients with bacterial vaginosis motile, anaerobic, comma-shaped bacteria can be isolated, which have recently been placed into the new genus mobiluncus. in this study, electron microscopy was used to examine the in situ adherence of these motile curved rods to detached epithelial cells (comma cells) in vaginal fluid from two patients with bacterial vaginosis. thin sections showed that the curved rods attached both directly to the epithelial cell surface and at various distances from it. i ... | 1988 | 3203257 |
| susceptibility of mobiluncus species to 23 antimicrobial agents and 15 other compounds. | the susceptibility of 12 strains of mobiluncus curtisii and 10 strains of m. mulieris to 23 antimicrobial agents and 15 other compounds was determined. all strains were susceptible to chloramphenicol, clindamycin, rifampin, tobramycin, vancomycin, virginiamycin, and all beta-lactam antibiotics tested, including imipenem. one strain of m. mulieris was resistant to erythromycin and josamycin. all were resistant to colistin, cycloserine, nalidixic acid, and neomycin. tetracycline had variable activ ... | 1987 | 3566250 |
| antigenic distinctiveness of mobiluncus curtisii and mobiluncus mulieris. | a total of 26 mobiluncus strains (17 m. curtisii and 9 m. mulieris strains) were compared serologically by double immunodiffusion and immunoblotting against antisera prepared against representative isolates of each species. all strains from the same species were strongly reactive with homologous antisera but generally weakly reactive with antisera to the heterologous mobiluncus spp. the antisera did not react with strains of the unrelated genera campylobacter, succinivibrio, wolinella, actinomyc ... | 1985 | 3924951 |
| nitrocellulose filter blots for species identification of mobiluncus curtisii and mobiluncus mulieris. | seventy strains of mobiluncus, motile curved anaerobic bacteria associated with bacterial vaginosis, were correctly identified to species level by using bacteria fixed to nitrocellulose and hybridized with 32p-labeled dna. | 1984 | 6541662 |
| effects of cetyltrimethylammonium naproxenate on the adherence of gardnerella vaginalis, mobiluncus curtisii, and lactobacillus acidophilus to vaginal epithelial cells. | a decreased concentration or total disappearance of lactobacillus acidophilus in the vagina constitutes a frequent observation in bacterial vaginosis. | 1994 | 7871448 |
| mobiluncus curtisii bacteremia following septic abortion. | | 1994 | 7888560 |
| bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome. | the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ... | 1994 | 8126176 |
| broad-range pcr amplification and dna sequence analysis reveals variable motifs in 16s rrna genes of mobiluncus species. | using dna primers based on highly conserved regions of bacterial 16s ribosomal rna genes, a technique was established for detection of mobiluncus species by polymerase chain reaction (pcr) and hybridization analysis. part of the 16s rrna genes of mobiluncus mulieris, mobiluncus curtisii and uncharacterized mobiluncus strains were analyzed by broad-range pcr amplification and direct dna sequencing analysis. sequence comparison of the partial 16s rrna genes of mobiluncus curtisii, mobiluncus mulie ... | 1995 | 8534436 |
| classification of the genus mobiluncus based on comparative partial 16s rrna gene analysis. | on the basis of partial 16s rrna gene sequences and the results of southern blot analyses, we confirmed the division of the genus mobiluncus into the species mobiluncus curtisii and mobiluncus mulieris. division of m. curtisii into m. curtisii subsp. curtisii and m. curtisii subsp. holmesii was not supported by our data. | 1996 | 8573515 |
| humoral antibody to mobiluncus curtisii, a potential serological marker for bacterial vaginosis. | while bacterial vaginosis (bv) is a polymicrobial syndrome, mobiluncus spp. are the organisms most highly associated with this condition. it is possible that serum antibody to mobiluncus spp. could be used as a serological marker for bv. using immunofluorescence techniques, we studied the prevalence of antibody to m. curtisii among three cohorts-pregnant women, pediatric patients, and sexually inexperienced women. the prevalence of antibody in each of these three groups was 75, 6, and 0%, respec ... | 1996 | 8877136 |
| antigenic profiles for the differentiation of mobiluncus curtisii and mobiluncus mulieris by immunoblotting technique. | the antigenic profile of 30 vaginal isolates of mobiluncus strains (22 m. curtisii and 8 m. mulieris) was determined by immunoblotting technique using mouse immune ascitic fluids containing polyclonal antibodies against the type strains m. curtisii subsp. holmesii (atcc 35242) and m. mulieris (atcc 35243). two antigenic profiles were identified within m. curtisii isolates, whereas a certain variability was observed among m. mulieris strains where at least three antigens were constantly recognize ... | 1997 | 9258945 |
| distribution and mobility of the tetracycline resistance determinant tetq. | we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ... | 1997 | 9372425 |
| severe sepsis caused by mobiluncus curtisii subsp. curtisii in a previously healthy female: case report and review. | we present the case of a 54-year-old female with life-threatening septicaemia due to mobiluncus curtisii subsp. curtisii. her admission was complicated by septic shock, renal failure, disseminated intravascular coagulation, the adult respiratory distress syndrome and spontaneous splenic rupture. the patient survived with full intensive care support and intravenous ceftriaxone. extra-genital infection with mobiluncus species is rarely diagnosed and has been confined to breast abscesses and non-li ... | 1998 | 9821100 |
| [clinical, epidemiological and microbiological aspects of mobiluncus sp. in bacterial vaginosis]. | in this paper, our goal was to determine the optimal isolation conditions, biochemical characterization, and preservation of species of the genus mobiluncus, associated with bacterial vaginosis in patients attending the family planning clinic. also, we tried to relate its presence with demographic variables and criteria used in the clinical diagnosis of bacterial diagnosis. | 1999 | 10396085 |
| isolation of mobiluncus species from the human vagina. | we report the results of a study concerning the characteristics of 52 strains of mobiluncus spp. isolated from 982 vaginal secretions from patients with suspected bacterial vaginosis. 158 of these women presented the features of this bacterial infection. of the strains isolated, 39 belonged to the species mobiluncus curtisii, (25 of these which to m. curtisii subsp. curtisii and 14, to m. curtisii subsp. holmesii), and 13, to mobiluncus mulieris. the vaginal isolates of mobiluncus spp. were iden ... | 2000 | 10705619 |
| evaluation of liquid culture media to support growth of mobiluncus species. | mobiluncus curtisii and m. mulieris are anaerobic, gram-negative, motile curved rods isolated commonly from the vagina of women with bacterial vaginosis. hitherto, there has been difficulty in isolating and growing these bacteria and little attention has been paid to growth in liquid media. reasons for establishing the means of attaining optimal growth in such media include production of antigens for diagnostic and immunological studies and production of the soluble cytotoxin. in this study the ... | 2002 | 12018656 |
| a multiplex polymerase chain reaction-based diagnostic method for bacterial vaginosis. | to develop a polymerase chain reaction (pcr)-based diagnostic method for bacterial vaginosis using bacterial vaginosis-associated anaerobes. | 2002 | 12383546 |
| transfer of members of the genus falcivibrio to the genus mobiluncus, and emended description of the genus mobiluncus. | it has long been thought that the genera mobiluncus and falcivibrio contain the same organisms. using a polyphasic approach, it was found that mobiluncus curtisii and mobiluncus mulieris were the same as falcivibrio vaginalis and falcivibrio grandis, respectively. as the genus name mobiluncus takes precedence, it is proposed that f. vaginalis and f. grandis be transferred to the genus mobiluncus. in agreement with previous studies, results from phenotypic tests did not support the separation of ... | 2004 | 15053324 |
| use of dna hybridization to detect vaginal pathogens associated with bacterial vaginosis among asymptomatic pregnant women. | the purpose of this study was to determine whether microbial dna hybridization is a useful method to study bacterial vaginosis in asymptomatic pregnant women. | 2005 | 16150270 |
| [vaginosis in patients with antenatal foetus death]. | polymerase chain reaction (pcr) analysis of bacterial vaginosis as well as of vaginal anaerobe flora (mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, bacteroides spp. and mobiluncus curtisii) was performed in women with antenatal foetus death. specimens from forty women with this pathology were studied. control group consisted of 100 pregnant women of the adequate age groups and gestation periods, but with live foetus. vaginal smears treated by the use of polymerase chain reac ... | 2006 | 17057305 |
| the lower genital tract microbiota in relation to cytokine-, slpi- and endotoxin levels: application of checkerboard dna-dna hybridization (cdh). | in the present study the lower genital tract microbiota in asymptomatic fertile women (n=34) was identified and quantified by culturing vaginal secretions. also, vaginal and cervical samples were analyzed by a semiquantitative checkerboard dna-dna hybridization technique (cdh) based on genomic probes prepared from 13 bacterial species (bacteroides ureolyticus, escherichia coli, fusobacterium nucleatum, gardnerella vaginalis, mobiluncus curtisii ss curtisii, prevotella bivia, prevotella disiens, ... | 2008 | 18397461 |
| association of mobiluncus curtisii with recurrence of bacterial vaginosis. | | 2008 | 18418301 |
| molecular quantification of gardnerella vaginalis and atopobium vaginae loads to predict bacterial vaginosis. | bacterial vaginosis (bv) is a poorly detected public health problem that is associated with preterm delivery and for which no reliable diagnostic tool exists. | 2008 | 18513147 |
| quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems. | transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ... | 2008 | 18579722 |
| microbiological findings in subjects with asymptomatic oral lichen planus: a cross-sectional comparative study. | the bacterial colonization of the oral mucosa was evaluated in patients with asymptomatic oral lichen planus (olp) and compared to the microbiologic status in mucosally healthy subjects. | 2008 | 19053926 |
| microflora of the penile skin-lined neovagina of transsexual women. | the microflora of the penile skin-lined neovagina in male-to-female transsexuals is a recently created microbial niche which thus far has been characterized only to a very limited extent. yet the knowledge of this microflora can be considered as essential to the follow-up of transsexual women. the primary objective of this study was to map the neo-vaginal microflora in a group of 50 transsexual women for whom a neovagina was constructed by means of the inverted penile skin flap technique. second ... | 2009 | 19457233 |
| sensitive detection of thirteen bacterial vaginosis-associated agents using multiplex polymerase chain reaction. | bacterial vaginosis (bv) is characterized by a polymicrobial proliferation of anaerobic bacteria and depletion of lactobacilli, which are components of natural vaginal microbiota. currently, there are limited conventional methods for bv diagnosis, and these methods are time-consuming, expensive, and rarely allow for the detection of more than one agent simultaneously. therefore, we conceived and validated a multiplex polymerase chain reaction (m-pcr) assay for the simultaneous screening of thirt ... | 2015 | 26078959 |
| bacterial vaginosis: a synthesis of the literature on etiology, prevalence, risk factors, and relationship with chlamydia and gonorrhea infections. | bacterial vaginosis (bv) is a common vaginal disorder in women of reproductive age. since the initial work of leopoldo in 1953 and gardner and dukes in 1955, researchers have not been able to identify the causative etiologic agent of bv. there is increasing evidence, however, that bv occurs when lactobacillus spp., the predominant species in healthy vaginal flora, are replaced by anaerobic bacteria, such as gardenella vaginalis, mobiluncus curtisii, m. mulieris, other anaerobic bacteria and/or m ... | 2016 | 26877884 |
| survival of vaginal microorganisms in three commercially available transport systems. | transport systems are used to collect and maintain the viability of microorganisms. two amies media based transport systems, bd cultureswabâ„¢ maxv(+) amies medium without charcoal (maxv(+)) and fisherfinest(®) with amies gel transport medium without charcoal (fisherfinest(®)) were compared to a cary-blair media based transport system, starswab(®) anaerobic transport system (starswab(®)), for their capacity to maintain the viability of 17 clinical microorganisms commonly isolated from the vagina ( ... | 2017 | 28242337 |
| combination of microbiological culture and multiplex pcr increases the range of vaginal microorganisms identified in cervical cancer patients at high risk for bacterial vaginosis and vaginitis. | bacterial vaginosis (bv) and vaginitis in cervical cancer patients might becaused by mixed aerobic, anaerobic, and atypical bacteria. since genital tract infections can be complicated, early and accurate identification of causal pathogens is vital. | 2015 | 26117968 |
| activity of novispirin g-10, a novel antimicrobial peptide against chlamydia trachomatis and vaginosis-associated bacteria. | we tested the activity of novispirin g-10, a novel antimicrobial alpha-helical octadecapeptide structurally related to cathelicidins and other innate immunity peptides, against chlamydia trachomatis serovars l2, d, and e and three organisms associated with bacterial vaginosis (bv). the peptide's activity against c. trachomatis was measured in 48-h shell vial assays with mccoy cell targets. exposure to 100 micro g/ml of novispirin g-10 reduced the infectivity of serovars d and e by 99.4-100% and ... | 2003 | 12710952 |
| identification and preliminary characterization of a cytotoxin isolated from mobiluncus spp. | mobiluncus curtisii and m. mulieris are anaerobic curved rods commonly isolated, together with other bacteria, from the vagina of women with bacterial vaginosis (bv). we have shown that of 11 strains of m. curtisii and four strains of m. mulieris examined, each produced a cytotoxin after growth in three types of liquid media. the toxin present in supernatant fluids after centrifugation of mobiluncus cultures was active against vero cells and four other cell lines tested, producing a marked cytop ... | 1993 | 8398808 |
| diversity of mobiluncus strains as demonstrated by their electrophoretic protein patterns. | the analysis of electrophoretic protein profiles of 21 mobiluncus curtisii, 11 mobiluncus mulieris and 3 reference strains (mobiluncus curtisii subsp. curtisii atcc 35241, mobiluncus curtisii subsp. holmesii atcc 35242 and mobiluncus mulieris bv 64-5) demonstrated species-related patterns. a highly variable region appeared at 70-85 kda for mobiluncus curtisii and at 75-95 kda for mobiluncus mulieris, which was likely to correspond to cell surface located proteins. when performed under standardiz ... | 1991 | 1789904 |
| mobiluncus curtisii bacteremia. | mobiluncus curtisii was isolated from the blood of a 35-year-old man with a medical history of ulcerative colitis who was admitted unconscious to the intensive care unit (icu). a ct scan revealed massive intracerebral hemorrhage in the left hemisphere. temperature remained constant over 38.5 degrees c; therefore, two sets of blood cultures were collected. one anaerobic bottle bact/alert sn (biomerieux, france) was detected as positive after 5 days of incubation and a gram stain confirmed a gram ... | 2008 | 18242106 |
| natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli. | the purpose of this study was to evaluate the ability of clindamycin and metronidazole to synergize with natural antimicrobials against biofilms of bacterial vaginosis (bv)-associated gardnerella vaginalis. minimum bactericidal concentrations for biofilm cells (mbcs-b) were determined for each antimicrobial. the mbcs-b of lauramide arginine ethyl ester (lae), subtilosin, clindamycin and metronidazole were 50, 69.5, 20 and 500 μg ml(-1), respectively. a checkerboard assay and isobologram were use ... | 2015 | 25838136 |
| establishment of a sensitive system for analysis of human vaginal microbiota on the basis of rrna-targeted reverse transcription-quantitative pcr. | ten specific primer sets, for lactobacillus gasseri, lactobacillus crispatus, atopobium vaginae, gardnerella vaginalis, mobiluncus curtisii, chlamydia trachomatis/muridarum, bifidobacterium longum subsp. longum, bifidobacterium longum subsp. infantis, bifidobacterium adolescentis, and bifidobacterium angulatum, were developed for quantitative analysis of vaginal microbiota. rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) analysis of the vaginal samples from 12 healthy japanese vol ... | 2015 | 25661498 |
| characterisation of the human uterine microbiome in non-pregnant women through deep sequencing of the v1-2 region of the 16s rrna gene. | background. it is widely assumed that the uterine cavity in non-pregnant women is physiologically sterile, also as a premise to the long-held view that human infants develop in a sterile uterine environment, though likely reflecting under-appraisal of the extent of the human bacterial metacommunity. in an exploratory study, we aimed to investigate the putative presence of a uterine microbiome in a selected series of non-pregnant women through deep sequencing of the v1-2 hypervariable region of t ... | 2016 | 26823997 |
| persistence and multiplication of obligate anaerobe bacteria in amebae under aerobic conditions. | after co-cultivation of mobiluncus curtisii, an obligate non-sporeforming anaerobe, with free living amebae from the acanthamoeba spp. under aerobic conditions, internalization, multiplication and persistence of bacterial cells were established for at least 4-6 weeks. under the same conditions and media without viable amebae, the cells of m. curtisii did not replicate and died in 4-7 days. the infection of amebae occurred with 10 to 100 bacteria per ml of co-cultivation media. in 7-14 days the a ... | 1999 | 16887658 |