| antimicrobial action of roquefortine. | roquefortine, a secondary metabolite of penicillium roqueforti, was investigated with regard to its effect on microorganisms. the growth of gram-positive bacteria was inhibited by roquefortine, whereas gram-negative bacteria were not influenced at all. the minimal inhibitory concentrations for gram-positive organisms were about 80 micrograms/ml cells on the average. the sensitive bacterium corynebacterium flaccumfaciens was chosen for characterizing the antimicrobial action of the mycotoxin. add ... | 1990 | 2231314 |
| nadp+ production using thermostable nad+ kinase of corynebacterium flaccumfaciens ahu-1622. | a sonicate of corynebacterium flaccumfaciens ahu-1622 had the highest nad+ kinase activity (1.22 mu/ml culture broth) of the strains of bacteria we investigated. this enzyme was thermostable, with activity maintained at 50 degrees c for 1 h. this treatment inactivated phosphatase activity. resting cells of the bacterium also had nad+ kinase activity when treated at 60 degrees c for 30 min with 0.2% triton x-100. nadp+ production was achieved using 8 mumol nad+, 8 mumol atp, 16 mumol mgcl2, 1.6 m ... | 1986 | 3017521 |
| the presence of acidic polysaccharides and muramic acid phosphate in the walls of corynebacterium poinsettiae and corynebacterium betae. | | 1974 | 4826548 |
| a purple-pigment-producing bean wilt bacterium, corynebacterium flaccumfaciens var. violaceum, n. var. | | 1968 | 5646843 |
| nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa. | thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ... | 1982 | 6183393 |
| fatty acid, isoprenoid quinone and polar lipid composition in the classification of curtobacterium and related taxa. | strains representing the taxa curtobacterium, brevibacterium saperdae, b. testaceum, corynebacterium betae, cor. nebraskense and cor. oortii were degraded by acid methanolysis and the non-hydroxylated fatty acid esters released were examined by thin-layer and gas-liquid chromatography. the major fatty acids in all strains were 12-methyltetradecanoic (anteiso c15) and 14-methylhexadecanoic (anteiso c17) acids which occurred together with other anteiso, iso and straight-chain acids. polar lipids o ... | 1980 | 6775048 |
| development of an improved assay for purine nucleoside kinase activity in cell extracts and detection of inosine kinase activity in brevibacterium acetylicum atcc 953, related species, and corynebacterium flaccumfaciens atcc 6887. | an improved assay was developed to detect direct purine nucleoside phosphorylating activity in cell-free extracts. direct inosine phosphorylating activity was detected in 2 of 70 species tested. both activities, which depended on magnesium ion and atp, phosphorylated a hydroxyl group at the 5' position of inosine. the new assay was shown to be useful for screening of direct purine nucleoside phosphorylating activity and have the potential to detect inosine kinase in the presence of a background ... | 2000 | 10830490 |
| variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks. | fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. the principal bacterial species isolated were alcaligenes sp., bacillus spp. (including b. cereus, b. lentus, b. megaterium, b. pumilus, and b. subtilis), burkholderia cepacia, curtobacterium flaccumfaciens, enterobacter cloacae, methylobacterium extorquens, and pantoea agglomerans, with p. agglomerans and b. pumilus being the most frequently isolated species. the most abundant fungal species wer ... | 2001 | 11315114 |
| effect of solar uv-b radiation on a phyllosphere bacterial community. | the effect of solar uv-b radiation on the population dynamics and composition of the culturable bacterial community from peanut (arachis hypogeae l.) was examined in field studies using plants grown under uv-b-transmitting (uv-b+) or uv-b-excluding (uv-b-) plastic filters. our data demonstrate that solar uv-b selection alters phyllosphere bacterial community composition and that uv tolerance is a prevalent phenotype late in the season. the total bacterial population size was not affected by eith ... | 2001 | 11722897 |
| diversity of endophytic bacterial populations and their interaction with xylella fastidiosa in citrus plants. | citrus variegated chlorosis (cvc) is caused by xylella fastidiosa, a phytopathogenic bacterium that can infect all citrus sinensis cultivars. the endophytic bacterial communities of healthy, resistant, and cvc-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. the endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (dgge). dominant isolates were characterized by fat ... | 2002 | 12324338 |
| pcr-based assay for the detection of curtobacterium flaccumfaciens pv. flaccumfaciens in bean seeds. | to develop a pcr-based protocol for the rapid, sensitive and specific detection of curtobacterium flaccumfaciens pv. flaccumfaciens (cff) in bean seeds. | 2002 | 12358698 |
| diversity of grass-associated microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of subtercola pratensis sp. nov., curtobacterium herbarum sp. nov. and plantibacter flavus gen. nov., sp. nov. | a representative selection of coryneform bacteria, isolated from the phyllosphere of grasses and the litter layer after mulching the sward, was characterized by a polyphasic approach to clarify their taxonomic position in the family microbacteriaceae, with particular reference to potentially plant-pathogenic bacteria. on the basis of 16s rdna analysis, the isolates can be classified into six genotypes representing the genera curtobacterium, clavibacter, subtercola and a subgroup, which was not a ... | 2002 | 12361245 |
| reconsideration of arthrobacter ilicis (mandel et al. 1961) collins et al. 1982 as a plant-pathogenic species. proposal to emend the authority and description of the species. request for an opinion. | strains now considered to represent the type strain of arthrobacter ilicis, described as a pathogen of american holly, are not identical. the designated type strain does not represent this pathogen. however, one of the other strains sourced to the type strain of the pathogen does appear to be authentic, but is not a member of a. ilicis. it is proposed that a. ilicis is an unrelated species, not a pathogen of american holly. the nomenclature of a. ilicis can be rectified by emending the authority ... | 2004 | 14742500 |
| interaction between endophytic bacteria from citrus plants and the phytopathogenic bacteria xylella fastidiosa, causal agent of citrus-variegated chlorosis. | to isolate endophytic bacteria and xylella fastidiosa and also to evaluate whether the bacterial endophyte community contributes to citrus-variegated chlorosis (cvc) status in sweet orange (citrus sinensis [l.] osbeck cv. pera). | 2004 | 15189288 |
| rapid, specific and quantitative assays for the detection of the endophytic bacterium methylobacterium mesophilicum in plants. | xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis disease in sweet orange. there is evidence that x. fastidiosa interacts with endophytic bacteria present in the xylem of sweet orange, and that these interactions, particularly with methylobacterium mesophilicum, may affect disease progress. however, these interactions cannot be evaluated in detail until efficient methods for detection and enumeration of these bacteria in planta are developed. we have previou ... | 2006 | 16266765 |
| conjugative plasmid in corynebacterium flaccumfaciens subsp. oortii that confers resistance to arsenite, arsenate, and antimony(iii). | gene transfer systems for phytopathogenic corynebacteria have not been reported previously. in this paper we describe a conjugative 46-megadalton plasmid (pdg101) found in corynebacterium flaccumfaciens subsp. oortii co101 that mediates resistance to arsenite, arsenate, and antimony(iii). transfer of the plasmid from co101 to four other strains from the c. flaccumfaciens group occurred between cells immobilized on nitrocellulose filters or on agar surfaces. transconjugant strains expressed the s ... | 1984 | 16346601 |
| [rapd analysis of plant pathogenic coryneform bacteria]. | rapd analysis was used for the taxonomy of plant pathogenic coryneform bacteria, especially for the classification of two new pathogens (curtobacterium flaccumfaciens pv. basellae pv. nov. and curtobacterium flaccumfaciens pv. beticola pv. nov.). 20 random primers were screened from 50 ones to detect polymorphism among the total strains used. 80.4% were polymorphic bands among the 225 ones produced. the results of pairwise similarity and upgma cluster analysis suggest that the two new pathovars ... | 2005 | 16496687 |
| a bacterial wilt of the bean caused by bacterium flaccumfaciens nov. sp. | | 1922 | 17758388 |
| medicinal smoke reduces airborne bacteria. | this study represents a comprehensive analysis and scientific validation of our ancient knowledge about the effect of ethnopharmacological aspects of natural products' smoke for therapy and health care on airborne bacterial composition and dynamics, using the biolog microplate panels and microlog database. we have observed that 1h treatment of medicinal smoke emanated by burning wood and a mixture of odoriferous and medicinal herbs (havan sámagri=material used in oblation to fire all over india) ... | 2007 | 17913417 |
| the endophyte curtobacterium flaccumfaciens reduces symptoms caused by xylella fastidiosa in catharanthus roseus. | citrus variegated chlorosis (cvc) is a disease of the sweet orange [citrus sinensis (l.)], which is caused by xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. sweet orange trees have been occasionally observed to be infected by xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. the principal endophytic bacterial species isolated from such cv ... | 2007 | 17978797 |
| curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. | a gram-positive, non-motile, pale-yellow, short rod-shaped bacterium, strain dcy26(t), was isolated from soil of a ginseng field in south korea and was investigated to determine its taxonomic position. the organism grew optimally at 30-37 degrees c. the g+c content of its dna was 65.8 mol%. phylogenetic analysis based on 16s rrna gene sequences revealed that strain dcy26(t) was related most closely to species of the genus curtobacterium, in the family microbacteriaceae. strain dcy26(t) showed hi ... | 2008 | 18842862 |
| mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. | abstract plant growth-promoting rhizobacteria (pgpr) strains inr7 (bacillus pumilus), gb03 (bacillus subtilis), and me1 (curtobacterium flaccumfaciens) were tested singly and in combinations for biological control against multiple cucumber pathogens. investigations under greenhouse conditions were conducted with three cucumber pathogens-colletotrichum orbiculare (causing anthracnose), pseudomonas syringae pv. lachrymans (causing angular leaf spot), and erwinia tracheiphila(causing cucurbit wilt ... | 1998 | 18944848 |
| comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of clavibacter michiganensis subsp. michiganensis. | detection of clavibacter michiganensis subsp. michiganensis (cmm), causing bacterial canker of tomato, was verified using pta-elisa and ifas with pabs of neogen europe ltd. (uk), and with published and also laboratory-generated pcr primers from the cmm tomatinase gene. the specificity of this technique was determined with 15 plant-pathogenic and 4 common, saprophytic bacteria. with ifas, crossreactions were found for pantoea dispersa, p. agglomerans and rahnella aquatilis, and with pta-elisa for ... | 2010 | 20526836 |
| [correlation of bacterial diversity in rot chinese cabbage with the habitat]. | to investigate the diversity of bacteria in soft rot chinese cabbage and analyze their correlation with rhizosphere bacteria, we analyzed the bacterial population structures of soft rot chinese cabbage and the rhizosphere in different habitat. | 2011 | 22379805 |
| isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (panicum virgatum l.) cultivars. | to isolate and characterize indigenous bacterial endophytes from cultivars of switchgrass and study their antimicrobial and growth promoting potential. | 2013 | 23190162 |
| paradox of plant growth promotion potential of rhizobacteria and their actual promotion effect on growth of barley (hordeum vulgare l.) under salt stress. | from the rhizosphere of two salt tolerant plant species, hordeum secalinum and plantago winteri growing in a naturally salt meadow, 100 strains were isolation on enrichment media for various plant growth-promoting (pgp) functions (acc deaminase activity, auxin synthesis, calcium phosphate mobilization and nitrogen fixation). based on the taxonomic affiliation of the isolated bacteria and their enrichment medium 22 isolates were selected to test their growth promotion effect on the crop barley (h ... | 2015 | 26640049 |
| [curtobacterium flaccumfaciens pv. flaccumfaciens is the agent of bacterial disease of soybean]. | a new phytopathogen has been identified and investigated as a result of ecosystem monitoring of bacterial soybean diseases in ukraine which is identified as curtobacterium flaccumfaciens pv. flaccumfaciens according to its phenotypic and genotypic characteristics. the pathogen is highly aggressive to soybean and can potentially be a threat of infection to other legumes. | 2014 | 24450181 |
| draft genome sequence of curtobacterium flaccumfaciens strain ucd-aku (phylum actinobacteria). | here we present the draft genome of an actinobacterium, curtobacterium flaccumfaciens strain ucd-aku, isolated from a residential carpet. the genome assembly contains 3,692,614 bp in 130 contigs. this is the first member of the curtobacterium genus to be sequenced. | 2013 | 23682147 |
| genetic diversity among curtobacterium flaccumfaciens pv. flaccumfaciens populations in the american high plains. | curtobacterium flaccumfaciens pv. flaccumfaciens is a gram-positive bacterium and has reemerged as an incitant of bacterial wilt in common (dry, edible) beans in western nebraska, eastern colorado, and southeastern wyoming. curtobacterium flaccumfaciens pv. flaccumfaciens is diverse phenotypically and genotypically and is represented by several different pathogen color variants. the population structure of 67 strains collected between 1957 and 2009, including some isolated from alternate hosts, ... | 2012 | 22642843 |
| characteristics of microbial biofilm on wooden vats ('gerles') in pdo salers cheese. | the purpose of this study was to characterize microbial biofilms from 'gerles' (wooden vats for making pdo salers cheese) and identify their role in milk inoculation and in preventing pathogen development. gerles from ten farms producing pdo salers cheese were subjected to microbial analysis during at least 4 periods spread over two years. they were distinguished by their levels of lactobacillus (between 4.50 and 6.01 log cfu/cm(2)), gram negative bacteria (between 1.45 and 4.56 log cfu/cm(2)), ... | 2012 | 22483401 |
| differential interactions between curtobacterium flaccumfaciens pv. flaccumfaciens and common bean. | bacterial wilt of common bean caused by curtobacterium flaccumfaciens pv. flaccumfaciens is an important disease in terms of economic importance. it reduces grain yield by colonizing xylem vessels, subsequently impeding the translocation of water and nutrients to the superior plant parts. the existence of physiological races in c. flaccumfaciens pv. flaccumfaciens has not so far been reported. the objective of the present investigation was to identify physiological races, evaluate differential i ... | 2016 | 27886332 |
| curtobacterium flaccumfaciens septic arthritis following puncture with a coxspur hawthorn thorn. | curtobacterium species are recognised plant pathogens. we report the first well-documented case of curtobacterium human infection, a child with septic arthritis following puncture with a coxspur hawthorn plant thorn. the organism isolated from synovial tissue and the plant thorn was identified as curtobacterium flaccumfaciens by 16s rrna gene sequence analysis. | 2011 | 21562106 |
| gene sequence phylogenies of the family microbacteriaceae. | the type strains of 32 species of 13 genera of the family microbacteriaceae were analysed with respect to gene-coding phylogeny for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca), and polyphosphate kinase (ppk). the resulting gene trees were compared with the 16s rrna gene phylogeny of the same strains. the topology of neighbour-joining and maximum parsimony phylogenetic trees, based on nucleic-acid sequences and protein sequences of housekeeping genes, differ ... | 2007 | 17551787 |
| development of a pcr test for the detection of curtobacterium flaccumfaciens pv. flaccumfaciens. | a chromosomal dna library of the bacterial pathogen of bean, curtobacterium flaccumfaciens pv.flaccumfaciens ncppb 559 was constructed in the plasmid pgem-7zf(+). several clones were identified that hybridised to all curtobacterium flaccumfaciens pathovars including: c. f betae, c. f flaccumfaciens, c. f oortii, c. f. poinsettiae and, in addition, to some strains of clavibacter michiganensis subsp. insidiosus and clavibacter michiganensis subsp. one of these clones (ppmp-26), after subsequent di ... | 2001 | 11761362 |
| isolation and identification of cellulolytic bacteria involved in the degradation of natural cellulosic fibres. | in search for bacterial cultures that are able to rapidly degrade cellulosic plant fibres in vitro, 77 cellulolytic strains were isolated from belgian and czech soils after enrichment on flax or sisal fibres as sole sources of carbon. the strains were characterized using fatty acid analysis, and 74 strains were grouped into three major clusters by numerical analysis. the first major cluster contained cellulomonas strains. within this cluster three subclusters could be delineated by principal com ... | 2000 | 10930083 |