| thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa. | acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, corynebacterium haemolyticum, corynebacterium pyogenes or from representatives of the genera arthrobacter, cellulomonas, curtobacterium, kurthia or oerskovia. thin-layer chromatography of whole-organism methanolysates ... | 1976 | 825611 |
| effect of natural and synthetic immunomodulators on the synthesis of interferon by peritoneal cells of mice. | the effect of different natural and synthetic immunomodulators on the spontaneous interferon (ifn) synthesis by freshly isolated resident peritoneal cells of balb/c, nzb and c3h mice was investigated. actinomycetal glycolipids isolated from curtobacterium betae, faenia rectivirgula, rothia dentocariosa and saccharopolyspora hirsuta at the concentration 1-20 micrograms/ml were found to potentiate the ifn synthesis by the peritoneal cells of balb/c mice. similar results were obtained when dsrna, l ... | 1992 | 1279963 |
| identification key for coryneform bacteria derived by numerical taxonomic studies. | six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ... | 1983 | 6413644 |
| fatty acid, isoprenoid quinone and polar lipid composition in the classification of curtobacterium and related taxa. | strains representing the taxa curtobacterium, brevibacterium saperdae, b. testaceum, corynebacterium betae, cor. nebraskense and cor. oortii were degraded by acid methanolysis and the non-hydroxylated fatty acid esters released were examined by thin-layer and gas-liquid chromatography. the major fatty acids in all strains were 12-methyltetradecanoic (anteiso c15) and 14-methylhexadecanoic (anteiso c17) acids which occurred together with other anteiso, iso and straight-chain acids. polar lipids o ... | 1980 | 6775048 |
| ccini, an isoschizomer of noti from curtobacterium citreum recognizes 5'-gc decreases ggccgc-3'. | ccini, an isoschizomer of noti, has been isolated from curtobacterium citreum. the enzyme cleaves within the recognition sequence 5'-gc decreases ggccgc-3' as indicated by the arrow. | 1995 | 7607535 |
| cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate "curtobacterium psychrophilum" inoue and komagata 1976. | "curtobacterium psychrophilum," proposed by inoue and komagata in 1976, is a psychrophilic gram-positive irregular rod isolated from antarctic soil. this organism grew optimally at 9 to 12 degrees c and did not grow at higher than 18 degrees c. chemotaxonomic characteristics of this organism were the presence of 2,4-diaminobutyric acid in the cell wall and menaquinone-10 as the predominant respiratory quinone. the cellular fatty acid profile, which contained a significant amount of an anteiso-br ... | 1997 | 9103638 |
| ultraviolet radiation (uvr) sensitivity analysis and uvr survival strategies of a bacterial community from the phyllosphere of field-grown peanut (arachis hypogeae l.). | > abstract the short-term population dynamics of the culturable bacterial community from field-grown peanut (arachis hypogeae l.) was analyzed over three 2-day periods. as in other phyllosphere studies, significant numbers of pigmented organisms were detected, suggesting the importance of pigmentation in the colonization of this habitat. isolates were grouped according to pigmentation (orange, pink, yellow, nonpigmented), and the sensitivity of each isolate in the collection (n = 617) to ultravi ... | 1999 | 10384007 |
| analysis of bacterial dna in skin and muscle of the tyrolean iceman offers new insight into the mummification process. | about 80 sequences (16s ribosomal rna gene) of bacterial dna in samples of skin and muscle taken directly from the tyrolean iceman (3350-3100 years b.c.) or recovered during the 1992 archaeological expedition at the alpine site were analyzed to obtain clues to the natural mummification process that allowed the corpse of the neolithic shepherd/hunter to be preserved for more than 5,000 years. the investigation was made more complex by the fact that the surface of the mummy had been swabbed with p ... | 2000 | 10640948 |
| variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks. | fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. the principal bacterial species isolated were alcaligenes sp., bacillus spp. (including b. cereus, b. lentus, b. megaterium, b. pumilus, and b. subtilis), burkholderia cepacia, curtobacterium flaccumfaciens, enterobacter cloacae, methylobacterium extorquens, and pantoea agglomerans, with p. agglomerans and b. pumilus being the most frequently isolated species. the most abundant fungal species wer ... | 2001 | 11315114 |
| effect of solar uv-b radiation on a phyllosphere bacterial community. | the effect of solar uv-b radiation on the population dynamics and composition of the culturable bacterial community from peanut (arachis hypogeae l.) was examined in field studies using plants grown under uv-b-transmitting (uv-b+) or uv-b-excluding (uv-b-) plastic filters. our data demonstrate that solar uv-b selection alters phyllosphere bacterial community composition and that uv tolerance is a prevalent phenotype late in the season. the total bacterial population size was not affected by eith ... | 2001 | 11722897 |
| characterization of a coryneform isolate from fasciated mugwort (artemisia vulgaris). | the morphological and physiological features of coryneform isolated from fasciated mugwort (artemisia vulgaris) display many morphological similarities with the plant pathogenic corynebacteria, but differ from corynebacterium fascians in exhibiting motility albeit in only a small proportion of each cell population, and by its ability to hydrolyze asculin, its failure to produce urease and differences in pigmentation. the isolate appears to be related to corynebacterium fascians in its ability to ... | 2002 | 12019725 |
| pcr-based assay for the detection of curtobacterium flaccumfaciens pv. flaccumfaciens in bean seeds. | to develop a pcr-based protocol for the rapid, sensitive and specific detection of curtobacterium flaccumfaciens pv. flaccumfaciens (cff) in bean seeds. | 2002 | 12358698 |
| interaction between endophytic bacteria from citrus plants and the phytopathogenic bacteria xylella fastidiosa, causal agent of citrus-variegated chlorosis. | to isolate endophytic bacteria and xylella fastidiosa and also to evaluate whether the bacterial endophyte community contributes to citrus-variegated chlorosis (cvc) status in sweet orange (citrus sinensis [l.] osbeck cv. pera). | 2004 | 15189288 |
| antibacterial activity of cuminum cyminum l. and carum carvi l. essential oils. | essential oils extracted by hydrodistillation from fruits of cuminum cyminum l. and carum carvi l. were analyzed by gas chromatography (gc) and gc-mass spectrometry (ms). the main components of c. cyminum oil were p-mentha-1,4-dien-7-al, cumin aldehyde, gamma-terpinene, and beta-pinene, while those of the c. carvi oil were carvone, limonene, germacrene d, and trans-dihydrocarvone. antibacterial activity, determined with the agar diffusion method, was observed against gram-positive and gram-negat ... | 2005 | 15631509 |
| diversity of endophytic bacterial populations and their interaction with xylella fastidiosa in citrus plants. | citrus variegated chlorosis (cvc) is caused by xylella fastidiosa, a phytopathogenic bacterium that can infect all citrus sinensis cultivars. the endophytic bacterial communities of healthy, resistant, and cvc-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. the endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (dgge). dominant isolates were characterized by fat ... | 2002 | 12324338 |
| [rapd analysis of plant pathogenic coryneform bacteria]. | rapd analysis was used for the taxonomy of plant pathogenic coryneform bacteria, especially for the classification of two new pathogens (curtobacterium flaccumfaciens pv. basellae pv. nov. and curtobacterium flaccumfaciens pv. beticola pv. nov.). 20 random primers were screened from 50 ones to detect polymorphism among the total strains used. 80.4% were polymorphic bands among the 225 ones produced. the results of pairwise similarity and upgma cluster analysis suggest that the two new pathovars ... | 2005 | 16496687 |
| culturable leaf-associated bacteria on tomato plants and their potential as biological control agents. | culturable leaf-associated bacteria inhabiting a plant have been considered as promising biological control agent (bca) candidates because they can survive on the plant. we investigated the relationship between bacterial groups of culturable leaf-associated bacteria on greenhouse- and field-grown tomato leaves and their antifungal activities against tomato diseases in vitro and in vivo. in addition, the isolated bacteria were analyzed for n-acyl-homoserine lactone (ahl) and indole-3-acetic acid ... | 2007 | 17356949 |
| isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. | endophytic bacteria reside within plant hosts without causing disease symptoms. in this study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop species and 27 prairie plant species. we determined several phenotypic properties and found approximately equal numbers of gram-negative and gram-positive isolates. in a greenhouse study, 28 of 86 prairie plant endophytes were found to colonize their original hosts at 42 days postinoculation at levels of 3.5 to 7.7 log(10) ... | 2002 | 11976089 |
| development of a pcr test for the detection of curtobacterium flaccumfaciens pv. flaccumfaciens. | a chromosomal dna library of the bacterial pathogen of bean, curtobacterium flaccumfaciens pv.flaccumfaciens ncppb 559 was constructed in the plasmid pgem-7zf(+). several clones were identified that hybridised to all curtobacterium flaccumfaciens pathovars including: c. f betae, c. f flaccumfaciens, c. f oortii, c. f. poinsettiae and, in addition, to some strains of clavibacter michiganensis subsp. insidiosus and clavibacter michiganensis subsp. one of these clones (ppmp-26), after subsequent di ... | 2001 | 11761362 |
| medicinal smoke reduces airborne bacteria. | this study represents a comprehensive analysis and scientific validation of our ancient knowledge about the effect of ethnopharmacological aspects of natural products' smoke for therapy and health care on airborne bacterial composition and dynamics, using the biolog microplate panels and microlog database. we have observed that 1h treatment of medicinal smoke emanated by burning wood and a mixture of odoriferous and medicinal herbs (havan sámagri=material used in oblation to fire all over india) ... | 2007 | 17913417 |
| the endophyte curtobacterium flaccumfaciens reduces symptoms caused by xylella fastidiosa in catharanthus roseus. | citrus variegated chlorosis (cvc) is a disease of the sweet orange [citrus sinensis (l.)], which is caused by xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. sweet orange trees have been occasionally observed to be infected by xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. the principal endophytic bacterial species isolated from such cv ... | 2007 | 17978797 |
| separation of plant pathogens from different hosts and tissues by capillary electromigration techniques. | in this contribution capillary isoelectric focusing and capillary zone electrophoresis were applied for the separation and detection of different plant pathogens including pseudomonas syringae pv. syringae, p. syringae pv. lachrymans, pseudomonas savastanoi pv. fraxinus, p. savastanoi pv. olea, agrobacterium tumefaciens, a vitis, xanthomonas arboricola pv. juglandis, x. campestris pv. zinniae, and curtobacterium sp.. the uv detection and sensitive fluorescence detection of the native phytopathog ... | 2007 | 17997525 |
| [microbiota of the orchid rhizoplane]. | six bacterial strains isolated from the underground roots of the terrestrial orchid calanthe vestita var. rubrooculata were found to belong to the genera arthrobacter, bacillus, mycobacterium, and pseudomonas. strains isolated from the aerial roots of the epiphytic orchid dendrobium moschatum were classified into the genera bacillus, curtobacterium, flavobacterium, nocardia, pseudomonas, rhodococcus, and xanthomonas. the rhizoplane of the terrestrial orchid was also populated by cyanobacteria of ... | 2001 | 11558285 |
| [dynamic of the volume and structure of the soil bacterial complex in the presence of azobenzene]. | azobenzene exerted no significant effect on the dynamics and the species composition of the saprophytic soil bacterial complex, which remained almost the same as in the control and was characterized by the predominance of curtobacterium sp., arthrobacter globiformis, and bacillus megaterium in all stages of succession. some heterotrophic bacteria were found to be able to accumulate azobenzene. bac. cereus and bac. polymyxa degraded azobenzene during their cultivation in nutrient media. | 2001 | 11558283 |
| isolation and identification of cellulolytic bacteria involved in the degradation of natural cellulosic fibres. | in search for bacterial cultures that are able to rapidly degrade cellulosic plant fibres in vitro, 77 cellulolytic strains were isolated from belgian and czech soils after enrichment on flax or sisal fibres as sole sources of carbon. the strains were characterized using fatty acid analysis, and 74 strains were grouped into three major clusters by numerical analysis. the first major cluster contained cellulomonas strains. within this cluster three subclusters could be delineated by principal com ... | 2000 | 10930083 |
| [studies on siderophore exchange properties between staphylococci and various species of gram-positive and gram-negative bacteria]. | the ability of iron utilizing by means of staphylococcal siderophores by bacteria belonging to genera: acinetobacter, corynebacterium, curtobacterium, clavibacter, bacillus and mycobacterium was investigated. the staphylococcal donor strains (18 species) used in these experiments were characterized by the ability to utilize siderophores produced by various strains belonging to aforenamed genera. the utilization of staphylococcal siderophores was studied on agar media in which minimally effective ... | 1999 | 10803251 |
| antimicrobial activity of o-carboranylalanine. | functionalized polyhedral carboranes, including amino acid analogs, have unique physicochemical properties and are used as experimental anticancer agents. however, our current knowledge on their effect in nonmammalian biological systems is limited. we investigated the activity spectrum in vitro of o-carboranylalanine (o-cba), considered to be a highly lipophilic analog of phenylalanine, against representative plant pathogenic bacteria and fungi of various taxonomic position. the antibacterial ef ... | 1999 | 10707765 |
| mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. | abstract plant growth-promoting rhizobacteria (pgpr) strains inr7 (bacillus pumilus), gb03 (bacillus subtilis), and me1 (curtobacterium flaccumfaciens) were tested singly and in combinations for biological control against multiple cucumber pathogens. investigations under greenhouse conditions were conducted with three cucumber pathogens-colletotrichum orbiculare (causing anthracnose), pseudomonas syringae pv. lachrymans (causing angular leaf spot), and erwinia tracheiphila(causing cucurbit wilt ... | 1998 | 18944848 |
| endophytic bacterial flora in root and stem tissues of black pepper (piper nigrum l.) genotype: isolation, identification and evaluation against phytophthora capsici. | to isolate and identify black pepper (piper nigrum l) associated endophytic bacteria antagonistic to phytophthora capsici causing foot rot disease. | 2009 | 19018963 |
| a cold-active extracellular metalloprotease from curtobacterium luteum (mtcc 7529): enzyme production and characterization. | a novel psychro-tolerant bacterium, curtobacterium luteum, secreting an extracellular protease was isolated from the soil of gangotri glacier, western himalaya. maximum enzyme production was achieved when the strain was grown in a ph-neutral medium containing skim milk at 15 degrees c over 120 h. the metal ions such as zn(2+) and cr(2+) enhanced enzyme production. the specific activity of purified enzyme was 8,090 units/mg after 34.1-fold purification. the 115 kda enzyme was a metalloprotease (a ... | 2008 | 19164881 |
| application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria. | strains belonging to the gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (tgge). this method allows the sequence-specific separation of amplified fragments of 16s rrna genes. a total of 115 reference strains representing the majority of the species of the genera aeromicrobium, agromyces, arthrobacter, aureobacterium, cellulomonas, curtobacterium, nocardioides and terrabacter were characterized. depending on the genus investigated, th ... | 1999 | 10028252 |
| genetic analysis of clavibacter toxicus, the agent of annual ryegrass toxicity. | multilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates of clavibacter toxicus, the agent of annual ryegrass toxicity. these included 37 western australian (wa) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in australia over approximately 8 years. seventeen reference strains for the related genera curtobacterium, rhodococcus and arthrobacter were examined for comparison. th ... | 1996 | 8870638 |
| polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge. | eleven isolates obtained from a laboratory sewage treatment plant, most of them presumptively assigned to the coryneform genera curtobacterium and aureobacterium were studied for the presence of intracellular polyphosphates and polyphosphate dependent enzymes. all isolates stored polyphosphates and showed adenylate kinase activities ranging from 64 to 815 mu mg-1. polyphosphate:amp phosphotransferase could only be detected in one isolate. three isolates showed a polyphosphate kinase activity als ... | 1993 | 8386121 |
| suprageneric classification of peptidoglycan group b actinomycetes by nucleotide sequencing of 5s ribosomal rna. | 5s ribosomal rna sequences were determined for thirteen actinomycetes mainly representatives with the rare group b type peptidoglycan. the primary and secondary structure of the resultant sequences were of the type characteristic of gram-positive bacteria with dna rich in guanine plus cytosine. the sequencing and associated chemotaxonomic data provide compelling grounds for classifying actinomycetes with a group b type peptidoglycan in a single family. the family microbacteriaceae fam. nov. is p ... | 1993 | 8085792 |
| endophytic bacterial diversity in grapevine (vitis vinifera l.) leaves described by 16s rrna gene sequence analysis and length heterogeneity-pcr. | diversity of bacterial endophytes associated with grapevine leaf tissues was analyzed by cultivation and cultivation-independent methods. in order to identify bacterial endophytes directly from metagenome, a protocol for bacteria enrichment and dna extraction was optimized. sequence analysis of 16s rrna gene libraries underscored five diverse operational taxonomic units (otus), showing best sequence matches with gamma-proteobacteria, family enterobacteriaceae, with a dominance of the genus panto ... | 2009 | 19763412 |
| metabolism of t-2 toxin in curtobacterium sp. strain 114-2. | the metabolic pathway of t-2 toxin in curtobacterium sp. strain 114, one of the t-2 toxin-assimilating soil bacteria, was investigated by thin-layer and gas-liquid chromatographic analyses. t-2 toxin added to the basal medium as a single carbon and energy source was biotransformed into ht-2 toxin and an unknown metabolite. infrared, mass spectrum, proton magnetic resonance, and other physico-chemical analyses identified this new metabolite as t-2 triol. t-2 toxin was first deacetylated by the ba ... | 1983 | 6614901 |
| nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa. | thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ... | 1982 | 6183393 |
| a polyphasic study on the species diversity of the sediment microbiota of lake hévíz. | lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ... | 2009 | 20038486 |
| characterization of the fatty acid synthetase system of curtobacterium pusillum. | curtobacterium pusillum contains 11-cyclohexylundecanoic acid as a major component of cellular fatty acids. a trace amount of 13-cyclohexyltridecanoic acid is also present. fatty acids other than omega-cyclohexyl fatty acids present are 13-methyltetradecanoic, 12-methyltetradecanoic, n-pentadecanoic, 14-methylpentadecanoic, 13-methylpentadecanoic, n-hexadecanoic, 15-methylhexadecanoic, 14-methylhexadecanoic, and n-heptadecanoic acids. the fatty acid synthetase system of this bacterium was studie ... | 1986 | 3528138 |
| phylogenetic analysis of the coryneform bacteria by 5s rrna sequences. | nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ... | 1987 | 3106318 |
| isoprenoid quinones in the classification of coryneform and related bacteria. | menaquinones were the only isoprenoid quinones found in 85 of the 95 coryneform bacteria examined. dihydromenaquinones having nine isoprene units were the main components isolated from corynebacterium bovis, from other glutamic acid-producing strains, and from arthrobacter globiformis and related species. dihydromenaquinones with eight isoprene units were found in brevibacterium linens, the remaining corynebacterium species and strains probably belonging to the genus rhodococcus. tetrahydromenaq ... | 1979 | 107269 |
| culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot. | microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ... | 2010 | 20657618 |
| rapid, specific and quantitative assays for the detection of the endophytic bacterium methylobacterium mesophilicum in plants. | xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis disease in sweet orange. there is evidence that x. fastidiosa interacts with endophytic bacteria present in the xylem of sweet orange, and that these interactions, particularly with methylobacterium mesophilicum, may affect disease progress. however, these interactions cannot be evaluated in detail until efficient methods for detection and enumeration of these bacteria in planta are developed. we have previou ... | 2006 | 16266765 |
| endophytic bacteria in coffea arabica l. | eighty-seven culturable endophytic bacterial isolates in 19 genera were obtained from coffee plants collected in colombia (n = 67), hawaii (n = 17), and mexico (n = 3). both gram positive and gram negative bacteria were isolated, with a greater percentage (68%) being gram negative. tissues yielding bacterial endophytes included adult plant leaves, various parts of the berry (e.g., crown, pulp, peduncle and seed), and leaves, stems, and roots of seedlings. some of the bacteria also occurred as ep ... | 2005 | 16187260 |
| first description of curtobacterium spp. isolated from human clinical specimens. | during a 4-year period, five strains (three of which were doubtless clinically significant) of yellow- or orange-pigmented, oxidative, slowly acid-producing coryneform bacteria were recovered from human clinical specimens in two reference laboratories or referred to them. the strains were motile, catalase positive, nitrate reductase negative, and urease negative, but strongly hydrolyzed esculin. in all reference and clinical strains described in the present study, anteisopentadecanoic (c(15:0ai) ... | 2005 | 15750056 |
| changes in bacterial flora of japanese cabbage during growth and potential source of flora. | bacterial flora of cabbage were identified and enumerated during various stages of growth, and the potential sources of contamination in the field were determined. bacterial counts increased from below the level of detection (2.4 log cfu/g) on seeds to 2.5 to 5.7 log cfu/g on seedlings. after transplanting, the counts of mesophilic aerobic bacteria on leaves decreased and then increased to 5.7 log cfu/g on outer leaves, 5.0 log cfu/g on middle leaves, and 3.0 log cfu/g on inner leaves at the har ... | 2011 | 21477482 |
| [isolation of endophytic bacteria in potato and test of antagonistic action to bacterial ring rot of potato]. | in this study, two hundred and forty bacterial strains were isolated from inner tissue of potato tubers collected from datong, taiyuan and inner mongolia autonomous regions. on the basis of antagonistic examination in vitro, fifty and five bacteria strains were characterized for antagonistic bacteria to ring rot of potato. it was 22.9 percentage of all bacteria strains. the biggest radius of suppression circle was 13 mm. nine strains were chosen for their suppression of bacterial ring rot, black ... | 2002 | 15346992 |
| gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of 'brevibacterium helvolum' dsm 20419 as pseudoclavibacter helvolus gen. nov., sp. nov. | a gram-positive, molinate-degrading bacterium, strain on4(t) (=dsm 13485(t)=lmg 21909(t)), was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. the strain was strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of 10-41 degrees c. it contained the major menaquinone mk-9 and a cell-wall peptidoglycan based on d-ornithine. 16s rdna sequence analysis revealed that the strain formed a distinct line of descent in the family m ... | 2004 | 15143025 |
| reconsideration of arthrobacter ilicis (mandel et al. 1961) collins et al. 1982 as a plant-pathogenic species. proposal to emend the authority and description of the species. request for an opinion. | strains now considered to represent the type strain of arthrobacter ilicis, described as a pathogen of american holly, are not identical. the designated type strain does not represent this pathogen. however, one of the other strains sourced to the type strain of the pathogen does appear to be authentic, but is not a member of a. ilicis. it is proposed that a. ilicis is an unrelated species, not a pathogen of american holly. the nomenclature of a. ilicis can be rectified by emending the authority ... | 2004 | 14742500 |
| fluctuation of bacteria isolated from elm tissues during different seasons and from different plant organs. | in this work we isolated a culturable endophytic aerobic heterotrophic bacterial community from the stem and root tissues of elm trees (ulmus spp.) and analyzed its fluctuations. a total of 724 bacterial isolates were collected at different times (april, june, september and december) from two elm trees, one infected with elm yellows phytoplasmas, and one which was healthy-looking. the isolates were grouped into 82 haplotypes, identified by means of amplified ribosomal dna restriction analysis (a ... | 2003 | 12648725 |
| diversity of grass-associated microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of subtercola pratensis sp. nov., curtobacterium herbarum sp. nov. and plantibacter flavus gen. nov., sp. nov. | a representative selection of coryneform bacteria, isolated from the phyllosphere of grasses and the litter layer after mulching the sward, was characterized by a polyphasic approach to clarify their taxonomic position in the family microbacteriaceae, with particular reference to potentially plant-pathogenic bacteria. on the basis of 16s rdna analysis, the isolates can be classified into six genotypes representing the genera curtobacterium, clavibacter, subtercola and a subgroup, which was not a ... | 2002 | 12361245 |
| gene sequence phylogenies of the family microbacteriaceae. | the type strains of 32 species of 13 genera of the family microbacteriaceae were analysed with respect to gene-coding phylogeny for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca), and polyphosphate kinase (ppk). the resulting gene trees were compared with the 16s rrna gene phylogeny of the same strains. the topology of neighbour-joining and maximum parsimony phylogenetic trees, based on nucleic-acid sequences and protein sequences of housekeeping genes, differ ... | 2007 | 17551787 |
| curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of vietnam. | the ammonia-producing bacteria b55(t), ca73, sa69 and sa72 were isolated from the waterweeds ludwigia adscendens (b55(t)) and eleocharis dulcis (ca73, sa69 and sa72) grown in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam. the isolates were gram-positive, irregular rod-shaped, non-spore-forming bacteria. on the basis of 16s rrna gene sequence similarity, strain b55(t) was shown to belong to the genus curtobacterium of the class actinobacteria. chemotaxonomic data (mk- ... | 2007 | 17625173 |
| diversity of endophytic bacterial communities in poplar grown under field conditions. | bacterial endophytes may be important for plant health and other ecologically relevant functions of poplar trees. the composition of endophytic bacteria colonizing the aerial parts of poplar was studied using a multiphasic approach. the terminal restriction fragment length polymorphism analysis of 16s rrna genes demonstrated the impact of different hybrid poplar clones on the endophytic community structure. detailed analysis of endophytic bacteria using cultivation methods in combination with cl ... | 2008 | 18199082 |
| characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients. | over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ... | 2008 | 18335166 |
| humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost. | a bacterial strain isolated from sewage sludge compost, strain sc-083t, was characterized. the isolate was a motile, gram-positive, short rod, forming coryneform v-shaped cells during the early stages of growth. the organism was strictly aerobic and able to grow between 22 and 36 degrees c and between ph 5.5 and 8.0. the predominant fatty acids were cyclohexyl-c17 : 0, anteiso-c17 : 0 and iso-c16 : 0, the major respiratory quinones were menaquinone 11 (mk-11) and 12 (mk-12), and the genomic dna ... | 2008 | 18398211 |
| curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. | a gram-positive, non-motile, pale-yellow, short rod-shaped bacterium, strain dcy26(t), was isolated from soil of a ginseng field in south korea and was investigated to determine its taxonomic position. the organism grew optimally at 30-37 degrees c. the g+c content of its dna was 65.8 mol%. phylogenetic analysis based on 16s rrna gene sequences revealed that strain dcy26(t) was related most closely to species of the genus curtobacterium, in the family microbacteriaceae. strain dcy26(t) showed hi ... | 2008 | 18842862 |
| characterization of the microbial community involved in the suppression of pythium aphanidermatum in cucumber grown on rockwool. | abstract the root pathogen pythium aphanidermatum induced lower levels of disease in cucumber (cucumis sativus) plants on unsterilized, re-used rockwool slabs than on heat-sterilized, re-used rockwool. several recolonization treatments of the sterilized rockwool enhanced the suppressiveness of the rockwool. microbial community structures in the different rockwool treatments were investigated by plate counts on selective media. disease suppressiveness in the different rockwool treatments showed t ... | 2005 | 18943014 |
| purification and properties of cold-active metalloprotease from curtobacterium luteum and effect of culture conditions on production. | curtobacterium luteum, a gram-positive psychrotrophic bacterium, secreting an extracellular protease was isolated from the soil of gangotri glacier, western himalaya. the maximum enzyme production was achieved when isolate was grown in a ph-neutral medium containing skim milk at 15 degrees c over 120 hour. the metal ions such as zn2+ and cr2+ enhanced enzyme production. the specific activity of purified enzyme was 8090 u/mg after 34.1 fold purification. the 115 kd enzyme was a metalloprotease (a ... | 2008 | 19306578 |
| proposal to transfer flavobacterium oceanosedimentum carty and litchfield 1978 to the genus curtobacterium as curtobacterium oceanosedimentum comb. nov. | flavobacterium oceanosedimentum carty & litchfield 1978(al) had been described as a novel species in the genus flavobacterium on the basis of phenotypic characteristics, although the organism shows a high g+c content, a property never found in the other members of the genus. in this study, we re-evaluated the taxonomic position of f. oceanosedimentum using a polyphasic approach. the 16s rrna gene sequence of f. oceanosedimentum atcc 31717(t) indicated that it is closely related to the genus curt ... | 2009 | 19459968 |
| occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland. | the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ... | 2009 | 19572487 |
| influence of temperature on the characteristics of aerobic granulation in sequencing batch airlift reactors. | aerobic granular sludge was cultivated in sequencing batch airlift reactors (sbar) at 25, 30, and 35 degrees c, respectively. the effect of temperature on the granules characteristics was analyzed and the microbial community structures of the granules were probed using scanning electron microscope (sem) and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). the results showed that 30 degrees c is optimum for matured granule cultivation, where the granules had a more co ... | 2009 | 19634435 |
| leaf-associated bacteria from transgenic white poplar producing resveratrol-like compounds: isolation, molecular characterization, and evaluation of oxidative stress tolerance. | the aim of this study was the isolation and characterization of the culturable bacteria inhabiting the leaves of transgenic white poplars (populus alba l. 'villafranca') engineered with the stsy gene for the production of resveratrol-like compounds. resveratrol glucosides are available in small amounts from natural sources or by expensive chemical synthesis procedures. an alternative approach for the large-scale production of these relevant pharmaceuticals is the use of transgenic plants as bior ... | 2009 | 19767855 |
| characterization of an endophytic bacterial community associated with eucalyptus spp. | endophytic bacteria were isolated from stems of eucalyptus spp (eucalyptus citriodora, e. grandis, e. urophylla, e. camaldulensis, e. torelliana, e. pellita, and a hybrid of e. grandis and e. urophylla) cultivated at two sites; they were characterized by rapd and amplified rdna restriction analysis (ardra). endophytic bacteria were more frequently isolated from e. grandis and e. pellita. the 76 isolates were identified by 16s rdna sequencing as erwinia/pantoea (45%), agrobacterium sp (21%), curt ... | 2009 | 19937585 |
| diversity of endophytic bacteria in brazilian sugarcane. | endophytic bacteria live inside plant tissues without causing disease. studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. we examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. potato-agar medium was used to cultivate the endophytes; 32 isolates were selected for analysis. dna was extracted and the 16s rrna gene was partially sequenced and used for molecular identific ... | 2010 | 20198580 |
| the characterization and diversity of bacterial endophytes of grapevine. | the diversity of culturable and nonculturable bacterial endophytes of grapevine (vitis vinifera l.) was examined using a combination of cultivation and molecular methods. entire grapevines were sampled to characterize bacterial diversity from different locations throughout the vine. gas chromatography of fatty acid methyl esters (fames) was used to identify culturable isolates prior to subsequent further microbiological characterization, whilst denaturing gradient gel electrophoresis (dgge) was ... | 2010 | 20453907 |
| production of folate by bacteria isolated from oat bran. | twenty bacteria isolated from three commercial oat bran products were tested for their folate production capability. the bacteria as well as some reference organisms were grown until early stationary phase on a rich medium (ypd), and the amount of total folate in the separated cell mass and the culture medium (supernatant) was determined by microbiological assay. folate vitamer distribution was determined for eight bacteria including one isolated from rye flakes. for seven bacteria the effect of ... | 2010 | 20708290 |
| isolation and characterization of hexavalent chromium-reducing rhizospheric bacteria from a wetland. | scirpus americanus pers. occurs naturally in "san germán," a pond that serves as a receptor of industrial wastewater in guanajuato, méxico. this plant accumulates metals mainly in the root: concentrations (mg/kg) of cr, as, cd and se were 970, 49, 41, and 85 respectively. analysis of rhizosphere samples indicated bacterial population of 10(8) cfu g(-1) in media with 0.2 mm cr(vi) and 10 mm sodium gluconate. thirteen isolates were obtained and phylogenetic analyses (16s rrna) indicated they corre ... | 2010 | 20734910 |
| curtobacterium flaccumfaciens septic arthritis following puncture with a coxspur hawthorn thorn. | curtobacterium species are recognised plant pathogens. we report the first well-documented case of curtobacterium human infection, a child with septic arthritis following puncture with a coxspur hawthorn plant thorn. the organism isolated from synovial tissue and the plant thorn was identified as curtobacterium flaccumfaciens by 16s rrna gene sequence analysis. | 2011 | 21562106 |
| redox properties of 8-quinolinol and implications for its mode of action. | 8-quinolinol (oxine, 8-hydroxyquinoline) is a simple aromatic alkaloid with allelopathic, antibacterial, antifungal, and cytotoxic activities. generally, it is assumed that 8-quinolinol toxicity depends on transition metal chelation that negatively affects their availability for metalloenzymes in the cell or reactive oxygen species generation (ros), which are formed following reduction of molecular oxygen by autoxidation of the redox active metal central atom of the 8-quinolinol complex. on the ... | 2011 | 21615015 |
| gyrb sequence analysis and maldi-tof ms as identification tools for plant pathogenic clavibacter. | the bacterial genus clavibacter has only one species, clavibacter michiganensis, containing five subspecies. all five are plant pathogens, among which three are recognized as quarantine pests (mentioned on the eppo a2 list). prevention of their introduction and epidemic outbreaks requires a reliable and accurate identification. currently, identification of these bacteria is time consuming and often problematic, mainly because of cross-reactions with other plant-associated bacteria in immunologic ... | 2011 | 21802235 |
| a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. | this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ... | 2011 | 21837472 |
| coexisting curtobacterium bacterium promotes growth of white-rot fungus stereum sp. | white-rot basidiomycetes are the main decomposers of woody biomass in forest ecosystems. little is known, however, about the interactions between white-rot fungi and other microorganisms in decayed wood. a wood-rotting fungus, stereum sp. strain tn4f, was isolated from a fruit body, and its coexisting cultivable bacteria were isolated from its substrate; natural white-rot decayed wood. the effects of bacteria on fungal growth were examined by confrontational assay in vitro. a growth-promoting ba ... | 2011 | 22101455 |
| gryllotalpicola gen. nov., with description of gryllotalpicola koreensis sp. nov., gryllotalpicola daejeonensis sp. nov., and gryllotalpicola kribbensis sp. nov., from the gut of gryllotalpa africana, and reclassification of curtobacterium ginsengisoli as gryllotalpicola ginsengisoli comb. nov. | strains ru-16t, ru-28, ru-04t and pu-02t were isolated from the gut of an insect, gryllotalpa africana. phylogenetic analyses based on 16s rrna gene sequences revealed that the strains belonged to the family microbacteriaceae. all the strains were most closely related to curtobacterium ginsengisoli dcy26t (below 97% 16s rrna gene sequence similarity). these isolates were gram-positive, motile (by gliding), rod-shaped and exhibited ivory-coloured colonies. their chemotaxonomic properties included ... | 2011 | 22140167 |
| comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of clavibacter michiganensis subsp. michiganensis. | detection of clavibacter michiganensis subsp. michiganensis (cmm), causing bacterial canker of tomato, was verified using pta-elisa and ifas with pabs of neogen europe ltd. (uk), and with published and also laboratory-generated pcr primers from the cmm tomatinase gene. the specificity of this technique was determined with 15 plant-pathogenic and 4 common, saprophytic bacteria. with ifas, crossreactions were found for pantoea dispersa, p. agglomerans and rahnella aquatilis, and with pta-elisa for ... | 2010 | 20526836 |
| isolation and characterization of folate-producing bacteria from oat bran and rye flakes. | the aim of this research was to identify endogenous bacteria in commercial oat bran and rye flake products in order to study their folate production capability while maintaining the soluble dietary fibre components in physiologically active, unhydrolyzed form. fourty-two bacteria were isolated from three different oat bran products and 26 bacteria from one rye flake consumer product. the bacteria were tentatively identified by sequence analysis of the 16s rrna genes. the identification results r ... | 2010 | 20678822 |