| phylogenetic analysis of the coryneform bacteria by 5s rrna sequences. | nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ... | 1987 | 3106318 |
| in situ probing of gram-positive bacteria with high dna g + c content using 23s rrna-targeted oligonucleotides. | 23s-rrna-targeted oligonucleotide probes were designed for the phylogenetic group 'gram-positive bacteria with high g + c content of dna' (gpbhgc). a sequence idiosyncrasy in two adjacent base pairs in the stem of helix 69 in domain iv of the 23s rrna is present in all hitherto analysed strains of gpbhgc. an oligonucleotide probe targeted to this region hybridized only with strains of gpbhgc and was successfully used for in situ monitoring of these cells in activated sludge. another unique featu ... | 1994 | 8000548 |
| union of the genera microbacterium orla-jensen and aureobacterium collins et al. in a redefined genus microbacterium. | the 16s rrna gene sequences of 19 strains, 11 strains representing validated aureobacterium or microbacterium species and eight strains of non-valid species or isolates, were determined. these sequences were aligned with the sequences of other validated aureobacterium and microbacterium species and related actinobacteria. a comparative sequence analysis of 43 strains revealed that the species of the genera aureobacterium and microbacterium form a monophyletic association in which species of both ... | 1998 | 9734028 |
| isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. | endophytic bacteria reside within plant hosts without causing disease symptoms. in this study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop species and 27 prairie plant species. we determined several phenotypic properties and found approximately equal numbers of gram-negative and gram-positive isolates. in a greenhouse study, 28 of 86 prairie plant endophytes were found to colonize their original hosts at 42 days postinoculation at levels of 3.5 to 7.7 log(10) ... | 2002 | 11976089 |
| microbacterium aerolatum sp. nov., isolated from the air in the 'virgilkapelle' in vienna. | three rod-shaped, gram-positive strains were isolated from the air of the chapel 'virgilkapelle' in vienna. a representative of these three strains, strain v-73t, shared the highest 16s rdna sequence similarities with members of the genus microbacterium, in particular microbacterium foliorum, microbacterium testaceum, microbacterium esteraromaticum, microbacterium keratanolyticum and microbacterium arabinogalactanolyticum. the strains displayed almost identical biochemical and physiological char ... | 2002 | 12148633 |
| sugar beet-associated bacterial and fungal communities show a high indigenous antagonistic potential against plant pathogens. | the aim of this study was to analyze microbial communities in/on sugar beet with special focus on antagonists toward plant pathogens. for this purpose, the composition of microorganisms isolated from the rhizosphere, phyllosphere, endorhiza, and endosphere of field-grown sugar beet plants was analyzed by a multiphasic approach at three different plant development stages at six locations in europe. the analysis of microbial communities by single strand conformation polymorphism (sscp) of 16s/18s ... | 2008 | 18060449 |
| plasmid transformation and expression of the firefly luciferase in microbacterium testaceum type and endophytic colonizing field strains. | microbacterium testaceum is a predominant endophytic bacterial species isolated from corn and sorghum in the midwestern united states. the development of genetic transfer systems for m. testaceum may enable its use for biocontrol and other applications. the type strain (ifo 12675) and field isolates (se017, se034, and ce648) were grown to mid-exponential phase, concentrated (1.0 x 1011 cfu x ml(-1)), electroporated (escherichia coli-clavibacter shuttle plasmid pdm302), and plated on tsa with 10 ... | 2008 | 18997853 |
| occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland. | the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ... | 2009 | 19572487 |
| novel n-acylhomoserine lactone-degrading bacteria isolated from the leaf surface of solanum tuberosum and their quorum-quenching properties. | we isolated and identified ahl-degrading bacteria from the leaf surface of solanum tuberosum. the 16 isolates inactivated both short- and long-chain ahls. two of these isolates, identified as microbacterium testaceum, showed putative ahl-lactonase activity. these two strains interrupted quorum-sensing dependent bacterial infection by plant pathogen pectobacterium carotovorum subsp. carotovorum. m. testaceum strains, which were isolated in this study, might be useful in the biocontrol of plant di ... | 2009 | 19734660 |
| aiim, a novel class of n-acylhomoserine lactonase from the leaf-associated bacterium microbacterium testaceum. | n-acylhomoserine lactones (ahls) are used as quorum-sensing signal molecules by many gram-negative bacteria. we have reported that microbacterium testaceum stlb037, which was isolated from the leaf surface of potato, has ahl-degrading activity. in this study, we cloned the aiim gene from the genomic library of stlb037, which has ahl-degrading activity and shows high homology with the alpha/beta hydrolase fold family from actinobacteria. purified aiim as a maltose binding fusion protein showed hi ... | 2010 | 20173075 |
| biochemical characterization of gram-positive and gram-negative plant-associated bacteria with micro-raman spectroscopy. | raman spectra of gram-positive and gram-negative plant bacteria have been measured with micro-raman spectrometers equipped with 785 and 514.5 nm lasers. the gram-positive bacteria microbacterium testaceum, paenibacillus validus, and clavibacter michiganensis subsp. michiganensis have strong carotenoid bands in the regions 1155-1157 cm(-1) and 1516-1522 cm(-1) that differentiate them from other tested gram-negative bacteria. in the raman spectrum of gram-positive bacteria bacillus megaterium exci ... | 2010 | 20412629 |
| description of a novel actinobacterium microbacterium assamensis sp. nov., isolated from water sample collected from the river brahmaputra, assam, india. | a gram-positive, yellow pigmented actinobacterium, strain s2-48(t) was isolated from water sample collected from the river brahmaputra, assam, india and subjected to a polyphasic taxonomic study. most of the physiological and biochemical properties, major fatty acids (c(15:0) anteiso, iso c(16:0) and c(17:0) anteiso), estimated dna g+c content (70.2 mol%) and 16s rrna gene sequence analysis showed that strain s2-48(t) belonged to the genus microbacterium. strain s2-48(t) exhibited highest 16s rr ... | 2010 | 21104080 |
| genome sequence of microbacterium testaceum stlb037, an n-acylhomoserine lactone-degrading bacterium isolated from potato leaves. | microbacterium testaceum is an endophytic gram-positive bacterium that resides within plant hosts. m. testaceum stlb037 was isolated from potato leaves and shows n-acylhomoserine lactone-degrading activity. here, we present the 3.98-mb complete genome sequence of stlb037, with an average gc content of 70.28%. | 2011 | 21357489 |
| microbacterium lemovicicum sp. nov., a bacterium isolated from a natural uranium-rich soil. | an actinobacterial strain, designated viu22(t), was isolated from a natural uranium-rich soil and was studied using a polyphasic approach. cells formed orange-pigmented colonies, were rod-shaped, gram-positive (non-staining method), non-motile and non-spore-forming. this organism grew in 0-4.5 % (w/v) nacl and at 15-37 °c, with optimal growth occurring in 0.5 % (w/v) nacl and at 30 °c. comparative 16s rrna gene sequence analysis revealed that the strain viu22(t) belonged to the genus microbacter ... | 2012 | 23264499 |
| phenotypic and genotypic properties of microbacterium yannicii, a recently described multidrug resistant bacterium isolated from a lung transplanted patient with cystic fibrosis in france. | cystic fibrosis (cf) lung microbiota consists of diverse species which are pathogens or opportunists or have unknown pathogenicity. here we report the full characterization of a recently described multidrug resistant bacterium, microbacterium yannicii, isolated from a cf patient who previously underwent lung transplantation. | 2013 | 23642186 |
| community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees. | cellulolytic bacteria in forest soil provide carbon sources to improve the soil fertility and sustain the nutrient balance of the forest ecological system through the decomposition of cellulosic remains. these bacteria can also be utilized for the biological conversion of biomass into renewable biofuels. in this study, the community compositions and activities of cellulolytic bacteria in the soils of forests planted with broad-leaved deciduous (chang qing garden, cqg) and broad-leaved evergreen ... | 2014 | 23893311 |
| endophytic bacteria isolated from common bean (phaseolus vulgaris) exhibiting high variability showed antimicrobial activity and quorum sensing inhibition. | endophytic bacteria play a key role in the biocontrol of phytopathogenic microorganisms. in this study, genotypic diversity was analyzed via repetitive element pcr (rep-pcr) of endophytic isolates of the phylum actinobacteria that were previously collected from leaves of cultivars of common bean (phaseolus vulgaris). considerable variability was observed, which has not been reported previously for this phylum of endophytic bacteria of the common bean. furthermore, the ethanol extracts from cultu ... | 2015 | 26202846 |
| microbacterium enclense sp. nov., isolated from sediment sample. | a novel bacterium (strain nio-1002(t)) belonging to the genus microbacterium was isolated from a marine sediment sample in chorao island, goa province, india. its morphology, physiology, biochemical features and 16s rrna gene sequence were characterized. cells of this strain were gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. it grew in 0-12% (w/v) nacl and at 25-37 °c, with optimal growth at 30 °c. phylogenetic analysis based on 16s rrna gene sequ ... | 2015 | 25829331 |
| classification of some coryneform bacteria in a new genus aureobacterium. | chemical, biochemical and morphological data indicate a close relationship between the taxa "microbacterium liquefaciens", arthrobacter flavescens, arthrobacter terregens, "corynebacterium barkeri", curtobacterium saperdae and curtobacterium testaceum. it is proposed that the above taxa be reclassified in a new genus aureobacterium, as aureobacterium liquefaciens nom. rev., comb. nov.; aureobacterium flavescens comb. nov.; aureobacterium terregens comb. nov.; aureobacterium barkeri nom. rev., co ... | 1983 | 23194596 |
| allohumibacter endophyticus gen. nov., sp. nov., isolated from the root of wild artemisia princeps (mugwort). | a novel actinobacterium designated strain mwe-a11t was isolated from the root of wild artemisia princeps (mugwort). the isolate was aerobic, gram-stain-positive and short rod-shaped, and the colonies were yellow and circular with entire margin. strain mwe-a11t grew at 15-37 °c and ph 6.0-8.0. the predominant isoprenoid quinones were mk-11 and mk-10. the predominant fatty acids were anteiso-c15:0 and iso-c16:0, and the dna g+c content was 68.8 mol%. the main polar lipids were diphosphatidylglycer ... | 2016 | 26842896 |
| haloalkalitolerant actinobacteria with capacity for anthracene degradation isolated from soils close to areas with oil activity in the state of veracruz, mexico. | the use of native strains of microorganisms from soils is an excellent option for bioremediation. to our knowledge, until now there has been no other group working on the isolation of actinobacteria from contaminated soils in mexico. in this study, samples of soils close to areas with oil activity in the state of veracruz, mexico, were inoculated for the isolation of actinobacteria. the strains isolated were characterized morphologically, and the concentrations of nacl and ph were determined for ... | 2016 | 27762425 |
| determination of the residual anthracene concentration in cultures of haloalkalitolerant actinomycetes by excitation fluorescence, emission fluorescence, and synchronous fluorescence: comparative study. | polycyclic aromatic hydrocarbons (pahs) are compounds that can be quantified by fluorescence due to their high quantum yield. haloalkalitolerant bacteria tolerate wide concentration ranges of nacl and ph. they are potentially useful in the pahs bioremediation of saline environments. however, it is known that salinity of the sample affects fluorescence signal regardless of the method. the objective of this work was to carry out a comparative study based on the sensitivity, linearity, and detectio ... | 2016 | 26925294 |
| isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (panicum virgatum l.) cultivars. | to isolate and characterize indigenous bacterial endophytes from cultivars of switchgrass and study their antimicrobial and growth promoting potential. | 2013 | 23190162 |
| diversity and distribution of n-acylhomoserine lactone (ahl)-degrading activity and ahl-lactonase (aiim) in genus microbacterium. | n-acylhomoserine lactone (ahl)-degrading enzyme, aiim, was identified from the potato leaf-associated microbacterium testaceum stlb037. in this study, we cloned eight aiim gene homologues from other ahl-degrading microbacterium strains. the similarity of the chromosomal locus of the aiim gene is associated with the phylogenetic classification based on 16s rrna. degenerate pcr revealed that the aiim gene was only conserved in ahl-degrading microbacterium strains, but not in fifteen microbacterium ... | 2012 | 22446311 |
| determination of microbial diversity in daqu, a fermentation starter culture of maotai liquor, using nested pcr-denaturing gradient gel electrophoresis. | this study endeavored to investigate the diversity of microbes present during the shaping, ripening and drying of daqu, a fermentation starter culture and substrata complex of maotai alcoholic spirit. a nested pcr-denaturing gradient gel electrophoresis technique was utilized with different combinations of primers. the results showed the presence of bacteria, yeasts and molds. the microflora, which originate from wheat, were readily detectable during every stage of the fermentation process. howe ... | 2012 | 22806111 |
| microbacterium hydrothermale sp. nov., an actinobacterium isolated from hydrothermal sediment. | a gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, strain 0704c9-2(t), was isolated from hydrothermal sediment of the indian ocean. the organism grew with 0-5% (w/v) nacl and at 10-37 °c, with optimal growth occurring with 1% nacl and at 28-30 °c. comparative 16s rrna gene sequence analysis revealed that strain 0704c9-2(t) belonged to the genus microbacterium. it exhibited highest 16s rrna gene sequence similarity with microbacterium testaceum dsm 20166(t) (98.4%). levels of simil ... | 2014 | 25052400 |