| yeast cell wall-dissolving enzymes of the thermotolerant actinomycete thermoactinomyces vulgaris. | the thermotolerant culture of thermoactinomyces vulgaris pa ii-4a was cultivated in the biotec fermenter to obtain the enzyme preparation with a high proteolytic and lytic activity. resistance of the cells of candida utilis and saccharomyces fragilis (mesophilic and thermotolerant strains) to the lytic action of the enzyme preparation was different. preliminary treatment of the yeast cells by l-cysteine increased their susceptibility to the lytic action of the preparation. the degree of lysis of ... | 1976 | 6860 |
| lipase productivity of a lipolytic strain of thermoactinomyces vulgaris. | an attempt was made to evaluate the lipase productivity by thermoactinomyces vulgaris with various nutritional and environmental factors. results suggest optimum conditions for production, viz. age of inoculum 18 hours, 55 degrees c, ph 6.8, 0.2% yeast extract, specific substrates, and shaken cultures incubated for 24--36 hours. lipases are induced by corn oil in presence of yeast extract or of certain substitutes; starch could replace these at 55 degrees c. a suggestion is given of a regulatory ... | 1978 | 34950 |
| identity and lipase activity of an isolate of thermoactinomyces vulgaris. | an actinomycete with thermophilic nature and strong lipolytic activity was isolated by enrichment from an egyptian soil. it proved to be thermoactinomyces vulgaris. variabilities justifying adansonian systematics were reported and discussed. the potential relation of an occasional drop in successfully resumed activity to an auto-inhibition phenomenon has been notified. optimum conditions for the activity of the extracellular lipase(s), including stability, suggest the practical importance of the ... | 1978 | 34951 |
| [characterization of a protease from thermoactinomyces vulgaris (thermitase). 3. substrate specificity and properties of partially purified thermitase]. | during the process of cultivation of th. vulgaris several proteases are formed. in the present investigation the extensively purified major component was used. the substrate specificity was determined by means of 7 proteins, 7 amino acid esters, 5 fatty acid esters and 15 amino acid 4-nitroanilides. among the protein substrates tested, urea denaturated hemoglobin was split best, followed by gelatin, casein, field bean protein, serum albumin and gluten. the weakest rate of hydrolysis was observed ... | 1978 | 34957 |
| [production and characterization of the proteases from thermoactinomyces vulgaris. iii. studies of protease formation in a small-scale pilot plant]. | | 1979 | 35884 |
| [production and characterization of proteases in thermoactinomyces vulgaris. iv. spectrum of extracellular proteases during cultivation]. | | 1979 | 39384 |
| chemical and serological comparison of two antigen extracts of thermoactinomyces candidus. | | 1979 | 86157 |
| antigenic relationships among thermophilic actinomycetes. | antigens from micropolyspora faeni, saccharomonospora viridis, thermoactinomyces candidus, t. sacchari and t. vulgaris were prepared by growing them on dialysate of trypticase soy broth. sera from rabbits immunized with these antigens were used to study cross-reactivity between thermophilic actinomycetes by antigen-antibody crossed immunoelectrophoresis and by agar gel double diffusion. mi. faeni and s. viridis showed some degree of cross-reaction, but both failed to show any cross reactivity wi ... | 1979 | 94466 |
| association of beta-glucosidase with intact cells of thermoactinomyces. | the location of the b-glucosidase activity in a whole culture broth of the thermophilic organism thermoactinomyces has been studied. little beta-glucosidase activity was found in the culture filtrate, while the culture solids contained the major part of the activity of the whole culture broth. the activity does not appear to be adsorbed to the culture solids; rather there is evidence that it is an intracellular soluble enzyme(s). the ph and temperature optima for a crude beta-glucosidase prepara ... | 1979 | 106906 |
| [incorporation of thymidine into the dna of actinomycetes. i. incorporation of exogenous thymidine into the dna of thermoactinomyces vulgaris]. | the incorporation of exogenous thymidine and thymine into acid-insoluble material of thermoactinomyces vulgaris has been studied during germination and subsequent growth. thymine is not incorporated. the incorporation of thymidine stops after a short time due to the rapid breakdown of thymidine to thymine and deoxyribose-1-phosphate by the inducible thymidine phosphorylase. deoxyadenosine enhances the incorporation of thymidine as well as of thymine and prolongs the tine of uptake. uridine stim ... | 1977 | 304639 |
| growth of thermoactinomyces. | | 1977 | 320356 |
| the diagnostic value of antibodies to the traditional antigens of farmer's lung in finland. | precipitating antibodies towards antigens associated with farmer's lung disease were studied in finnish dairy farming and non-farming rural populations and the results were compared to those of serum samples of patients suspected of having a fungal allergy. the antigen panel consisted of aspergillus fumigatus, micropolyspora faeni and thermoactinomyces vulgaris. all three microbes seemed to be important environmental inducers of antibody formation. the prevalence of antibodies towards all of the ... | 1978 | 361284 |
| [production and characterization of proteases of thermoactinomyces vulgaris. i. proteases of thermophilic microorganisms (a review)]. | | 1978 | 362740 |
| humidifier-associated extrinsic allergic alveolitis. | three cases of allergic alveolitis due to indoor humdification systems are described. thermoactinomyces vulgaris precipitins were detected in the serum of a 37-year-old female patient who had typical febrile attacks during exposure to cool-mist from a home humidifier. when the cause was detected and eliminated, the symptoms and signs disappeared and the woman's gas transfer factor improved from 56% to normal within six months. in a printing office a 60-year-old woman had had febrile attacks with ... | 1979 | 375386 |
| [effect of oils and fatty acids on growth and enzyme production of thermoactinomyces vulgaris. i. effect of oils and fatty acids on the metabolism of microorganisms (review of literature)]. | | 1979 | 397684 |
| indirect immunofluorescent detection of antibodies against thermophilic actinomycetes in patients with hypersensitivity pneumonitis. | with slide culture preparations of micropolyspora faeni, thermoactinomyces candidus, and thermoactinomyces vulgaris used as antigens, 59 sera from patients with hypersensitivity pneumonitis and 23 sera from normal controls were studied for the presence of specific antibodies by indirect immunofluorescence and immunodiffusion methods. of the 82 sera, 31 showed precipitins to m. faeni, five to t. candidus, and four to t. vulgaris. a group of 19 sera positive to aspergillus antigens showed question ... | 1977 | 402432 |
| epoxidation of aldrin to exo-dieldrin by soil bacteria. | twenty-two strains of soil bacteria, including representatives of the genera bacillus, micromonospora, mycobacterium, nocardia, streptomyces, thermoactinomyces, and pseudomonas and 10 unidentified gram-negative, motile, rod-shaped bacteria, were shown to degrade aldrin to its epoxide dieldrin. in every case, the exo-stereoisomer of dieldrin was produced exclusively. | 1977 | 407844 |
| microbiological and serological studies of farmers' lung in finland. | sera from few finnish patients with clinical farmers' lung react in precipitin tests with extracts of the thermophilic actinomycetes that commonly cause the disease elsewhere. hays associated with the disease in finland showed less evidence of spontaneous heating and contained fewer actinomycete spores than british hays. only thermoactinomyces vulgaris was sometimes abundant. some species of mesophilic fungi were more abundant than in britain and one, aspergillus umbrosus, reacted with most sera ... | 1979 | 421335 |
| [production and the characterization of the proteases from thermoactinomyces vulgaris. ii. the selection of productive strains]. | | 1979 | 442700 |
| [substrate specificity of a protease from thermoactinomyces vulgaris]. | the cleavage specificity of a protease from thermoactinomyces vulgaris (thermitase) was determined by the insulin b-chain and the cleavability of casein and haemoglobin by this enzyme as compared to other proteases (trypsin, chymotrypsin, proteases from bac. megaterium and cytophages). the most intense splitting effect on the substrates under investigation (insulin b-chain, casein and haemoglobin) is exerted by thermitase, i. e., the unspecificity of this enzyme is especially marked. | 1979 | 460396 |
| properties of thermoactinomyces vulgaris phage ta1 and its extracted dna. | the virulent phage ta1 was obtained in good yields from infected cultures of thermoactinomyces vulgaris 1227. the purified phage was found to sediment with a single band, the sedimentation constant being (519 +/- 14)s, and to exhibit a typical nucleoprotein behaviour in uv-spectrophotometric and cd experiments. the ta1 phage consists of a hexagonal head about 0.056 micrometers in diameter and a very short tail. it is morphologically similar to the temperate salmonella phage p22. the nucleic acid ... | 1979 | 516794 |
| crossed immunoelectrophoretic analysis of two antigen extracts of thermoactinomyces candidus. | a pyridine extract antigen and a double-dialysis antigen (dda) obtained from thermoactinomyces candidus were analyzed by crossed immunoelectrophoresis. in addition, the heat lability, pronase sensitivity, and isolectric points of the components of the dda were determined. by using antisera raised against crude pyridine extract antigen, two immunogenic components were resolved by crossed immunoelectrophoresis. a similar analysis of dda using antisera raised against crude dda revealed 15 immunogen ... | 1979 | 528048 |
| [studies on the composting of feathers (author's transl)]. | the nitrogen of keratin of feathers is not taken up by plants as a suitable n-source. it becomes available by the keratinolytic activity of microorganisms. the high temperature evolved during the composting process promotes the growth and activity of thermophilic, keratin-decomposing microorganisms, e.g. thermoactinomyces vulgaris. the increasing in temperature (self-heating) reflects the course of composting. a feather-peat mixture exhibits a considerably high heat production, i.e. a fast decom ... | 1978 | 569407 |
| [lipolytic activity from milk isolated strains of the species thermoactinomyces vulgaris, tsiklinsky 1899 (author's transl)]. | thirty strains of the species thermoactinomyces vulgaris (tsiklinsky, 1988) were isolated from milk and tested for their lipolythic activity. after incubation for 120 hours at 55 degrees c they formed distinct diffusion zones on culture media containing tributyrin and tween-80. in all strains exocellular lipases were found. their action on the milk fat increased the number of the free fatty acids. triglycerides, free fatty acids as well as diglycerides and monoglycerides were identified as split ... | 1979 | 573533 |
| studies on thermoactinomyces sp. tm9208. i. description of the strain. | a strain of thermoactinomyces sp., tm9208, isolated from a soil sample in the taipei area showed antagonistic activity to gram positive bacteria but not to the gram negative on the potato extract agar plate by the cross streaking method. the strain showed strong starch hydrolysis, beta type of hemolysis, brownish yellow growth on the potato extract agar and green on the nutrient agar. its aerial mycelium, white to grayish white, was long and straight with short branches. the spores are single an ... | 1977 | 578130 |
| [the thermophilic streptomycetes flora in milk powders and condensed milk products (author's transl)]. | 247 specimens of powdered milk and 165 of condensed milk were tested for their contamination with thermophile streptomycetes. colonies of these contaminants were isolated from all specimens of powdered milk and from 73 samples of condensed sweeted milk. the isolated strains corresponded with the following species of thermophilic streptomycetes: thermoactinomyces vulgaris, tsiklinsky 1899, thermoactinomyces vulgaris, tsiklinsky 1899 "giant colonies", micromonospora sp. (agre et al., 1). | 1978 | 581428 |
| occurrence and thermoresistance of thermophilic streptomycetes spores in milk. | 200 samples of raw milk and 100 samples of pasteurized milk were tested for the presence of thermophilic streptomycetes. one ml of raw and pasteurized milk contained mean 8.42 and 14.95 thermophilic streptomycetes, respectively. the isolated strains belonging to the species thermoactinomyces vulgaris tsiklinsky 1899 and micromonospore sp. (agre et al., 1972) were identified. to determine the survival of spores of these thermophiles after pasteurization, samples of milk were heated at 63.5 degree ... | 1978 | 581429 |
| production of thermophilic actinomycete-hay aerosols for use in experimental hypersensitivity pneumonitis. | an investigation of the factors that influence the production of dense aerosols of micropolyspora faeni and thermoactinomyces vulgaris from hay cultures revealed that the density, freshness, and moisture content of the hay were important influences. dry aerosols were produced under optimum conditions from hay cultures of either actinomycete and from sterile hay for inhalation challenges to unimmunized rabbits. depressions in arterial oxygen tensions and hemolytic complement were monitored after ... | 1977 | 596873 |
| [isolation, crystallization and partial characterization of a cationic protease from thermoactinomyces vulgaris]. | a simple method was developed for the isolation of the cationic endopeptidase from a crude extract prepared from the culture medium of thermoactinomy ces vulgaris, consisting in chromatography on sephadex g 75 and subsequent separation on cm-sephadex in 50 mm tris/hcl, ph 8, using a nacl-gradient (0 - 0,2 m). this procedure results in a 4,2 fold increase of the elastolytic activity (substrate: n-acetyl-(l-ala)3methyl ester) of the enzyme. it moves as a single band in sds-gel electrophoresis and ... | 1977 | 612086 |
| elimination of bagassosis in louisiana paper manufacturing plant workers. | the prevalence of bagassosis was investigated in a louisiana paper mill, which in the past had considerable numbers of workers with the disease. based on negative clinical histories and the low number of positive serological reactions of the workers' serum with thermoactinomyces sacchari antigen, it was concluded that bagassosis was no longer present. this was thought to be due to a different method of storage of bagasse, which retards microbial decay and reduces airborne organic dust, and to th ... | 1978 | 627039 |
| [viability study of actinomycete cultures preserved for a long time on agar media]. | viability of 254 actinomycetous strains stored for 1 to 6 years on agarized media a temperature of 4-10 degrees c without passages was studied. the cultures included 204 strain of actinomyces, 26 strains of mycobacterium, 2 strains of streptosporangium, 1 strain of intrasporangium, 2 strains of micropolyspora and 5 strains of thermoactinomyces. most of the cultures, i.e. 94.55 per cent stored for 6 years remained vialbe after the this period. with an increase in the storage period from 1 to 4 ye ... | 1978 | 655683 |
| characterization of thermoactinomyces sacchari antigens. | three different extracts of thermoactinomyces sacchari were analyzed for their antigenicity and physical-chemical properties. rabbit antiserum to each preparation, tested by immunodiffusion in gel, demonstrated that the most potent immunogen was that prepared by the double-dialysis method. this extract also contained the greatest number of precipitating antigens as detected by gel filtration. all extracts, analyzed by column chromatography on sephadex g-75, were heterogeneous in that they contai ... | 1978 | 669809 |
| isolation anc characterization of actinophages of thermoactinomyces and micropolyspora. | two new actionphages infecting thermophilic actinomycetes were isolated from lysogenic cultures of thermoactinomyces candidus and micropolyspora faeni. the physiochemical properties, morphology, host range, electron microscopy, and immunology of the two phages, phi-115a (atcc 29680-b1) and phi-150a (atcc 29681-b1), are reported. | 1978 | 679066 |
| [variability of the proteolytic activity in the thermotolerant actinomycete, thermoactinomyces vulgaris]. | the thermophilic culture of thermoactinomyces vulgaris pa-11-4a, like other actinomycetes, is characterized by a high variability. this strain produces protease. the property is maintained if spores are incubated in sterilized distilled water for 30 min after lyophilization and then inoculated onto a solid medium (petri plates) in order to select small dark-coloured colonies. the activity of protease does not decrease if such colonies are grown on solid media during seven months at 4 degrees c. | 1978 | 703646 |
| transition of thermoactinomyces substrate mycelium from growth to sporulation. | | 1978 | 735168 |
| [characterization of a protease from thermoactinomyces vulgaris (thermitase). 1. purification of thermitase]. | the paper deals with the purification of the microbial protease preparation "thermitase" (submerged cultivation of thermoactinomyces vulgaris; treatment of culture filtrate with ethanol or na2so4, vacuum drying of precipitate). the crude substance was purified by column chromatography on sephadex g-75, deae-cellulose and sephadex g-50. the proteolytically active fractions were in each case united, freeze dried and tested for protein components and protease activity by gel electrophoresis. after ... | 1978 | 749455 |
| [characterization of a protease from thermoactinomyces vulgaris (thermitase). 2. single-step fine purification and protein-chemical characterization]. | the fine purification of an alkaline protease (thermitase) from thermoactinomyces vulgaris by means of isoelectrical focussing in the flat-bed procedure using granulated gel is reported. an na2so4-precipitated crude product serves as the starting material. isoelectrical focussing leads in a single step to a highly purified protein with an uniform n-terminal end group. the enzyme has an ip at 9.0 and a mol. wt. of 37,400; it consists of a polypeptide chain with arginine as the n-terminal, and tyr ... | 1978 | 749456 |
| kinetics of vegetative growth of thermoactinomyces vulgaris. | the growth of thermoactinomyces vulgaris on solid media was studied by microculture technique. starting from a germinating spore the further development was followed by phase contrast microscopy. from serially taken photographs the kinetics of growth and branching of the mycelium was calculated. also some observations on morphological behaviour were made. regarding the total mycelium developing from one spore the growth occurred witha constant exponential growth rate, at least for the time teste ... | 1978 | 753045 |
| antibody responses in patients with farmer's lung disease to antigens from thermoactinomyces vulgaris. | a serological analysis of mycelial antigens of thermoactinomyces vulgaris in immunodiffusion with human sera revealed five individual antigens. three antigens were proteins, sensitive to pronase and soluble in phenol. two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid. latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies to t. vulgaris; the number of positive results detected by each test differe ... | 1975 | 803522 |
| high incidence of precipitins in sera of heroin addicts. | we analyzed sera of 50 consecutively hospitalized heroin-abuse patients for precipitins against several antigens. forty-seven addicts had precipitins against extracts from moldy hay, and 34 against extracts from bagasse. thirty-six showed precipitin bands against extracts from one or more of the following: aspergillus, micropolyspora faeni, and thermoactinomyces vulgaris. these findings were significantly different from those of a control population (p less than .0001 for all antigens tested). ... | 1975 | 805851 |
| immunologic cross-reactions among thermophilic actinomycetes associated with hypersensitivity pneumonitis. | antibodies produced in rabbits against micropolyspora faeni, thermoactinomyces vulgaris, t. sacchari, thermoactinomyces candidus, and saccharomonospora viridis were tested against antigens derived from many strains of thermophilic actinomycetes for precipitating antibodies by immunodiffusion test. it was found that immune sera reacted strongly against antigens from strains belonging to the same species and weakly against antigens from different species of thermophilic actinomycetes. however, ser ... | 1976 | 816841 |
| [causes of degeneration of cultures of thermoactinomyces vulgaris]. | passage of cultures of thermoactinomyces vulgaris on a peptone-maize medium causes their degeneration which is manifested in an impaired formation of the sporulating aerial mycelium, and in an increase of the amount of non-germinating spores in populations. the process of degeneration depends on the following conditions: the location of the inoculated material (spores) on the surface of a solid growth medium, which is determined by the technique of inoculation; the state of the spores (degenerat ... | 1977 | 882014 |
| [spores of degenerating cultures of thermoactinomyces vulgaris]. | | 1977 | 882023 |
| isolation of bacteriophage from thermoactinomyces. | bacteriophages were isolated from strains of thermoactinomyces vulgaris, t. candidus, and t. sacchari used to produce antigen for hypersensitivity pneumonitis screening at the marshfield medical foundation. whereas the one phage isolated from t. sacchari and two phages from t. vulgaris were species specific, three other phages isolated from t. vulgaris and the two phages isolated from t. candidus were infectious for both t. vulgaris and t. candidus, thus indicating a close relationship between t ... | 1977 | 914995 |
| precipitating antibodies in a midwest dairy farming population toward the antigens associated with farmer's lung disease. | a survey of the frequency of precipitins to the antigens of the thermophilic actinomycetes and aspergillus species was conducted on serum samples from 1,045 farmers obtained at a 3-day exposition on modern farm equipment and farming practices in central wisconsin. each farmer filled out a questionnaire including socioeconomic information, lung disease history, exposure history, and smoking history. precipitins were detected by the double-diffusion method. the antigen panel included eight thermop ... | 1976 | 932340 |
| letter: thermoactinomyces in refrigerators. | | 1976 | 947042 |
| the effect of respiratory immunization on cell-mediated immune effector cells of the lung. | in order to understand the mechanisms of cellular immune injury in hypersensitivity pneumonitis, the effect of type of antigen on cell-mediated immunity in guinea-pigs receiving respiratory immunization was studied. lymphocytes obtained by pulmonary lavage were compared with those from peritoneal exudate following immunization with either a soluble protein, human serum albumin, or a particulate suspension of thermoactinomyces vulgaris. assays were obtained without mixing cells from these two sou ... | 1976 | 949869 |
| [protein hydrolysis by immobilized enzymes]. | the enzymic hydrolysis of some proteins (insulin-b-chain-s-sulfonate, s-aminoethylated lysozyme, bovine serum albumin) by immobilized peptidolytic enzymes is reported. sepharose-bound pronase, trypsin and a protease from thermoactinomyces sp. (mp), the latter both cross linked by glutaric dialdehyde and an exopeptidase mixture containing sepharose-bound leucine aminopeptidase, carboxypeptidase a and a crude preparation of prolidase were used. after enzymic hydrolysis nearly all amino acids, exce ... | 1976 | 983621 |
| home humidifier thermophilic actinomycete isolates. | twenty-seven thermophilic actinomycete isolates obtained from humidification systems or living-bedroom areas of individuals with suspected but unproved home environment-related respiratory disease were characterized morphologically and biochemically. all isolates were demonstrated to be members of the thermoactinomyces genus. two previously misidentified isolates were reclassified as a thermoacetinomyces sp. thus, all of our thermophilic actinomycete humidifier isolates studied to date have been ... | 1976 | 1078519 |
| [development of thermoactinomyces vulgaris on a synthetic medium]. | a synthetic medium was used to obtain the dormant spores of thermoactinomyces vulgaris. the fraction of the dormant spores depended on the amino acid composition of the growth medium. the rate of growth and development of the organism on the synthetic medium is lower as compared to the routinely employed complex medium. | 1975 | 1177783 |
| use of a serologically distinct strain of thermoactinomyces vulgaris in the diagnosis of farmer's lung disease. | during investigations into farmer's lung disease it was noticed that micropolyspora faeni (mf) was isolated and identified with ease, but difficulty was encountered in the identification of thermoactinomyces vulgaris (tv), although an organism resembling tv was frequently isolated. extracts prepared from the isolates resembling tv (called thermoactinomyces vulgaris variant, tvv) when tested against the standard tv antiserum by double diffusion, did not produce any precipitin lines. when tvv extr ... | 1975 | 1206112 |
| [temperature regimes for the formation and germination of thermoactinomyces vulgaris endospores]. | | 1975 | 1206150 |
| [effect of the composition of the medium and magnesium and calcium ions on the germination of spores of thermoactinomyces vulgaris]. | the germination of the spores of thermoactinomyces vulgaris formed on a complex medium is stimulated by suspending them in solutions containing mg2+ and ca2+ ions. the stimulation is not the result of the initiation of the spores in the presence of the ions since the experiments were carried out at a temperature of 20 degrees c at which the initiation did not virtually take place. the ions of na+ and k+ have almost no effect on the germination of the spores. the fraction of the resting spores of ... | 1975 | 1214606 |
| a scheme for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. | a scheme has been developed for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. eighty strains, 10 micropolyspora faeni, 6 saccharomonospora viridis, 52 thermoactinomyces candidus, 7 t. vulgaris, 4 t. sacchari, and 1 t. dichotomica, either isolated from patients' environment or received as authentic strains, were studied. in addition to the cultural and microscopic morphology in various media, each strain was subjected to an array of biochemical tes ... | 1975 | 1225928 |
| a quantitative study on the activation of the alternative pathway of complement by mouldy hay dust and thermophilic actinomycetes. | materials associated with the induction of farmer's lung were incubated with fresh normal human serum in the presence of magnesium ethylene glycol tetra-acetic acid (mgegta) or ethylene diamine tetra-acetic acid (edta) and results compared with material known to activate the alternative pathway of complement--zymosan. results show that micropolyspora faeni organisms are as active as zymosan in reducing complement (c) levels in the presence of mgegta, with a 50% reduction in ch50 at approximatel ... | 1976 | 1248097 |
| activation of the complement sequence by extracts of bacteria and fungi associated with hypersensitivity pneumonitis. | complement (c) consumption by antigens associated with hypersensitivity pneumonitis was investigated. micropolyspora faeni in the presence of precipitating antibodies consumed c in a manner similar to the bsa-anti bsa system. an extract of m. faeni was shown to consume c and convert c3pa to c3a in the absence of detectable antibodies. in addition, three species of aspergillus and two strains of thermoactinomyces vulgaris were also shown to have the same activity with respect to c3pa. mucor racem ... | 1976 | 1262608 |
| the value of precipitating antibodies in screening for hypersensitivity pneumonitis. | to evaluate the usefulness of precipitin tests as a screening method to detect hypersensitivity pneumonitis, we compared persons with precipitins to organic antigens with precipitin-negative subjects from the same population of 1,072 office workers participating in a health survey examination. thirty-three of the 53 persons with preciptins to micropolyspora faeni, thermoactinomyces vulgaris, t. candidus, pigeon serum, aspergillus, alternaria, pullularia, penicillium, cephalosporium, trichoderma, ... | 1976 | 1267251 |
| alkaloid from thermoactinomyces species. | | 1976 | 1269064 |
| comparison of methods of production of farmer's lung antigens. | the extracellular growth products of micropolyspora faeni, thermoactinomyces vulgaris and thermoactinomyces candidus were prepared by three methods; (1) double dialysis; (2) growth in tryptone soya broth (tsb) and (3) synthetic broth (aoac). it was found that double dialysis was superior to growth in tsb or aoac for the production of m. faeni antigens, as determined by immunoblot. double dialysis, tsb and aoac were similar for antigens of t. vulgaris and t. candidus. both organisms showed bands ... | 1992 | 1282842 |
| purification and characterization of a thermostable alkaline protease from alkalophilic thermoactinomyces sp. hs682. | protease secreted into the culture medium by alkalophilic thermoactinomyces sp. hs682 was purified to an electrophoretically homogeneous state through only two chromatographies using butyl-toyopearl 650m and sp-toyopearl 650s columns. the purified enzyme has an apparent relative molecular mass of 25,000 according to gel filtration on a sephadex g-75 column and sds-page and an isoelectric point above 11.0. its proteolytic activity was inhibited by active-site inhibitors of serine protease, dfp an ... | 1992 | 1368301 |
| [exogenous allergic alveolitis: assessment of antigen-specific igg antibodies using a new chemiluminescence method]. | the demonstration of elevated levels of antigen-specific igg antibodies is of importance for the diagnostic workup of patients with a suspected hypersensitivity pneumonitis. we established a solid phase immunoassay for the determination of these antibodies based on chemiluminescence (clia). the clia demonstrated a low level of unspecific binding. the reproducibility of the clia was evaluated by calculating the intra- and inter-variation; the variation coefficient was 4% (intra-variation) resp. 1 ... | 1992 | 1372432 |
| [serine proteinase from the archaebacterium halobacterium mediterranei--an analog of eubacterium subtilisin]. | a homogeneous serine proteinase was isolated from cultural filtrates of the extreme halophilic bacteria halobacterium mediterranei 1538 using affinity chromatography on bacitracin-sepharose, ultrafiltration and gel filtration on sephadex g-75, with a 48% yield and 260-fold purification. the enzyme was completely inactivated by specific inhibitors of serine proteinases, pmsf and dfp, as well as by hg2+ and pcmb. the enzyme activity was strongly dependent of nacl concentration, the enzyme being in ... | 1992 | 1391225 |
| primary structure of carboxypeptidase t: delineation of functionally relevant features in zn-carboxypeptidase family. | the primary structure of carboxypeptidase t--a zn-dependent extracellular enzyme of thermoactinomyces vulgaris--was determined from the cloned cpt gene nucleotide sequence and compared to zn-carboxypeptidases from various organisms. the compilation and analysis of multiple alignment accompanied by consideration of available tertiary structure data have shown that in the overall spatial structure and active site arrangement cpt is similar to other enzymes constituting the zn-carboxypeptidase fami ... | 1992 | 1449602 |
| farmer's lung disease in north-western india--a preliminary report. | a study of farmer's lung (fl) disease was carried out in 197 subjects engaged in farming and having respiratory complaints of varying duration. it revealed that 13.2% of the subjects had precipitating antibodies against thermophilic actinomycetes, with faenia rectivirgula (micropolyspora faeni) alone accounting for 85% of the positive reactions. precipitating antibodies against thermoactinomyces vulgaris and t. thalpophilus were observed only in 1.5% and 0.5% of the subjects, respectively. two s ... | 1992 | 1459662 |
| [case report of farmer's lung disease in siblings in kagoshima prefecture]. | two cases of farmer's lung disease in siblings are reported. a 54-year-old male farmer, who had been engaged in stock work for 20 years, presented to our clinic for the second episode of fever, productive cough and shortness of breath. chest roentgenogram revealed diffuse micronodular pattern, and mild hypoxemia was recognized on arterial blood gas analysis. cytology obtained from balf showed lymphocytosis, with especially increased okt3, okt4 positive cells and okt4/8 ratio. the diagnosis was c ... | 1992 | 1464985 |
| [changes in type ii alveolar epithelial cells in experimental allergic alveolitis]. | changes of type ii alveolar epithelial cells (type ii cells) were studied in experimental allergic alveolitis by using enzyme histochemical, electron microscopic and morphometric techniques. the results showed that the number of type ii cells increased obviously after exposed to thermoactinomyces vulgaris (tv). the peak was on the 15th day and then decreased gradually till to normal on the 180th day. type ii cells could be divided into two populations in tv-treated rats, namely, the large type i ... | 1992 | 1473211 |
| hypersensitivity pneumonitis in athymic nude mice. additional evidence of t cell dependency. | we previously demonstrated that c57 black/6 mice develop lung lesions similar to human hypersensitivity pneumonitis (hp) by repeated transnasal administration of thermoactinomyces vulgaris (tv) antigen. to elucidate the role of t cells in the development of this disease, tv antigen (90 micrograms/day) was transnasally administered to athymic nude c57 black/nu/nu (nu) mice and their littermates (+/nu) three times a week for 3 wk. the nude mice developed minimal lung lesions, whereas their thymus- ... | 1992 | 1489144 |
| crystal structure of carboxypeptidase t from thermoactinomyces vulgaris. | the crystal structure of carboxypeptidase t from thermoactinomyces vulgaris has been determined at 0.235-nm resolution by x-ray diffraction. carboxypeptidase t is a remote homologue of mammalian zn-carboxypeptidases. in spite of the low degree of amino acid sequence identity, the three-dimensional structure of carboxypeptidase t is very similar to that of pancreatic carboxypeptidases a and b. the core of the protein molecule is formed by an eight-stranded mixed beta sheet. the active site is loc ... | 1992 | 1521526 |
| a serine proteinase of an archaebacterium, halobacterium mediterranei. a homologue of eubacterial subtilisins. | a homogeneous serine proteinase secreted by the extreme halophilic bacterium halobacterium mediterranei 1538 was isolated by affinity chromatography on bacitracin-sepharose with a yield of 48% (260-fold purification). the enzyme reveals an optimum for pyroglutamyl-ala-ala-leu p-nitroanilide hydrolysis at ph 8.0-8.5 (km 0.14 mm; k(cat). 36.9 s-1). its activity increases linearly with nacl concentration over the range 2-5 m. the substrate specificity of the enzyme is comparable with that of secret ... | 1992 | 1637313 |
| molecular cloning and sequencing of the gene for a halophilic alkaline serine protease (halolysin) from an unidentified halophilic archaea strain (172p1) and expression of the gene in haloferax volcanii. | the gene of a halophilic alkaline serine protease, halolysin, from an unidentified halophilic archaea (archaebacterium) was cloned and its nucleotide sequence was determined. the deduced amino acid sequence showed that halolysin consists of 411 amino acids, with a molecular weight of 41,963. the highest homology was found with thermitase from thermoactinomyces vulgaris. halolysin has a long c-terminal extension of approximately 120 amino acids which has not been found in other extracellular subt ... | 1992 | 1732209 |
| type iii hypersensitivity reaction in mushroom growers. | some respiratory symptoms in mushroom growers such as mushroom worker's lung develop by inhalation of certain agents arising from the environment of mushroom cultivation. recently we observed mushroom workers who had respiratory symptoms which might be type iii hypersensitivity reaction to the antigen of pleurotus floridae. we gave questionnaires to all the mushroom growers at one of the biggest cultivation areas of mushrooms, pleurotus floridae in pocheon, kyunggi province. those with respirato ... | 1991 | 1742253 |
| [a study on interleukin 2 and soluble interleukin 2 receptor in serum and bronchoalveolar lavage fluid from patients with farmer's lung]. | interleukin 2 (il-2) and soluble interleukin 2 receptor (il-2r) were assayed in serum and bronchoalveolar lavage fluid (balf) from patients with farmer's lung. patients with farmer's lung had significantly higher levels of soluble il-2r in serum and balf than normal controls. the level of soluble il-2r was correlated with the disease activity, and fell to within the normal range after successful treatment with corticosteroids. on the other hand, asymptomatic dairy farmers with precipitating anti ... | 1991 | 1770680 |
| thermophilic actinomycetes associated with agro-environment of punjab state (india). | thermophilic actinomycetes (tas) are unique high temperature aerobic bacteria which belong to the group actinomycetes. while working on hypersensitivity pneumonitis (hp), an immunological disorder, resulting from the inhalation of spores of thermophilic actinomycetes, it was considered worthwhile to record their occurrence in the environment of punjab. thermophilic actinomycetes isolated by spread plate method from nine different substrates of the punjab environment were identified as 8 differen ... | 1991 | 1813626 |
| studies on properties of thermostable beta-amylase from thermoactinomyces sp.v4. | a thermostable beta-amylase from thermoactinomyces sp. v4 isolated from soil is reported. the enzyme has optimum activity at 70 degrees c and ph6. it is stable at 50 degrees c (4h) and retains 92% of activity at 55 degrees c (2h). the extent of hydrolysis soluble starch is about 77% and the product is identified as beta-maltose by paper chromatography and rotatory examination. sulfhydryl reagent has no effect on the beta-amylase activity. | 1991 | 1839610 |
| occurrence and significance of precipitating antibodies against thermophilic actinomycetes in the sera of dairy herd workers, nangali, delhi. | the study was prompted by the lack of information on the role of thermophilic actinomycetes in hypersensitivity pneumonitis in india. it reports the occurrence of precipitating antibodies against clinically important thermophilic actinomycetes in the sera of a population sample of dairy herd workers, nangali, delhi. of 112 workers investigated, 28 (25%) showed precipitins against faenia rectivirgula, 4 (3.2%) against saccharomonospora viridis, 2 against thermoactinomyces thalpophilus and one eac ... | 1991 | 1867472 |
| thermostable phenylalanine dehydrogenase of thermoactinomyces intermedius: cloning, expression, and sequencing of its gene. | the gene encoding the thermostable phenylalanine dehydrogenase [ec 1.4.1.-] of a thermophile, thermoactinomyces intermedius, was cloned and its complete dna sequence was determined. the phenylalanine dehydrogenase gene (pdh) consists of 1,098 nucleotides and encodes 366 amino acid residues corresponding to the subunit (mr 41,000) of the hexameric enzyme. the amino acid sequence deduced from the nucleotide sequence of the pdh gene of t. intermedius was 56.0 and 42.1% homologous to those of the ph ... | 1991 | 1880121 |
| [immunologic response to microorganisms from a polluted ventilation system in a working environment]. | authors point out the morbidity of employees working in ventilation systems contaminated with various microorganisms. they analysed 96 workers exposed to air conditioning system (group a), and 71 workers (group b) breathing normal ambient air. the workers of both groups were subjected clinically by functionally and immunologically. preparation of antigens "mmm" (monday morning miseries) was used as an original method by ajello et al. for producing antigens from systemic mycotic agents and subseq ... | 1991 | 1916435 |
| molecular cloning and primary structure of thermoactinomyces vulgaris carboxypeptidase t. a metalloenzyme endowed with dual substrate specificity. | a gene coding for an extracellular zn-carboxypeptidase of thermoactinomyces vulgaris has been cloned and sequenced (embl x56901). this enzyme named carboxypeptidase t reveals simultaneously both types of substrate specificity characteristic of mammalian carboxypeptidases a and b. the carboxypeptidase t gene is primarily expressed in e. coli as a non-active preproenzyme with an additional 98 amino acid residues at the n-terminus. primary structure alignment of mature carboxypeptidase t and mammal ... | 1991 | 1936254 |
| [a thermostable alpha-amylase from thermoactinomyces vulgaris: purification and characterization]. | alpha-amylase from thermoactinomyces vulgaris (strain 94-2a) was purified by cellulose chromatography and gel-chromatography on sephadex g-75 and subsequently characterised. the enzyme shows a single band in the polyacrylamide gel electrophoresis (page). the isoelectric point was determined to be ph 5.4, and the molecular mass was estimated as 53,000 dalton by page. the amino acid composition was determined; it shows characteristics of other microbial alpha-amylases. a comparison of the n-termin ... | 1991 | 1953690 |
| circulating antibodies against thermophilic actinomycetes in farmers and mushroom workers. | sera from individuals exposed to moldy hay, mushroom compost and unexposed individuals, were studied for antibodies against thermophilic actinomycetes and aspergillus fumigatus. antigens extracted from thermoactinomyces vulgaris strains isolated from china were used in the study along with antigens from standard strains to screen the sera of individuals exposed to farms and unexposed controls from both china and the united states. it was found that very little antibody response to a. fumigatus a ... | 1991 | 1960394 |
| distribution, purification, and characterization of thermostable phenylalanine dehydrogenase from thermophilic actinomycetes. | phenylalanine dehydrogenase (l-phenylalanine:nad oxidoreductase, deaminating; ec 1.4.1.-) was found in various thermophilic actinomycetes. we purified the enzyme to homogeneity from thermoactinomyces intermedius ifo 14230 by heat treatment and by red sepharose 4b, deae-toyopearl, sepharose cl-4b, and sephadex g-100 chromatographies with a 13% yield. the relative molecular weight of the native enzyme was estimated to be about 270,000 by gel filtration. the enzyme consists of six subunits identica ... | 1991 | 2061279 |
| crystal structure of thermitase at 1.4 a resolution. | the crystal structure of thermitase, a subtilisin-type serine proteinase from thermoactinomyces vulgaris, was determined by x-ray diffraction at 1.4 a resolution. the structure was solved by a combination of molecular and isomorphous replacement. the starting model was that of subtilisin bpn' from the protein data bank, determined at 2.5 a resolution. the high-resolution refinement was based on data collected using synchrotron radiation with a fuji image plate as detector. the model of thermitas ... | 1990 | 2196375 |
| [crystal structure of thermitase and stability of subtilisins]. | crystal structure of thermitase, a serine proteinase from thermoactinomyces vulgaris, has been determined by x-ray diffraction at 1.4 a resolution. the atomic model of thermitase refined to an r-factor of 0.149 contains 1997 protein atoms, 182 water molecules and 2 ca2+ ions. the tertiary structure of thermitase is similar to that of subtilisin bpn'. the greatest variations are connected with insertions and deletions in the amino acid sequence, which are located on the surface of the molecule. h ... | 1990 | 2198028 |
| viable endospores of thermoactinomyces vulgaris in lake sediments as indicators of agricultural history. | bacteria of the genus thermoactinomyces form endospores with an extreme longevity in natural habitats. we isolated thermoactinomyces sacchari from 9,000-year-old varved (annually laminated) sediment; thus, t. sacchari is probably one of the oldest known living organisms. more importantly, we tested and verified the hypothesis that there is a relationship between concentrations of dormant, viable endospores of t. vulgaris in lake sediments and the extent of agriculture in the catchments of the la ... | 1990 | 2202253 |
| [study of the thrombolytic and fibrinolytic properties of thiol- dependent serine proteinase (tsp) from thermoactinomyces vulgaris in vivo]. | experiments on male albino rats showed that the thyol-dependent serine proteinase (tsp) dissolved the thrombus in the jugular vein for 2-5 hr. intravenous injection of tsp activated the fibrinolytic system of intact animals by increasing the levels of the plasminogen activator and plasmin in the euglobulin fraction. the response of the fibrinolytic system on the intravenous injection of tsp (2mg/200 g) was different: in some rats, fibrinolysis was activated, while on others, it was inhibited. ts ... | 1990 | 2281048 |
| serum igg antibody concentrations against environmental microbes in mares and foals during different seasons and effect of stabling practices. | over periods of 22 and 14 months, igg antibody concentrations in serum samples obtained monthly from 14 mares and 19 foals, respectively, were measured by use of elisa against antigens of the following environmental microbes: aspergillus umbrosus, penicillium brevicompactum, rhodotorula glutinis, absidia corymbifera, aspergillus fumigatus, humicola grisea, micropolyspora faeni, and thermoactinomyces vulgaris. the mares and foals were on pasture from early june until early october, then were stab ... | 1990 | 2327613 |
| class-specific antibodies during follow up of patients with farmer's lung. | sequential serum samples of 13 patients with acute farmer's lung (fl) taken during a follow-up of 18-36 months, were tested for antibodies of immunoglobulin g (igg), iga, igm and ige classes against thermoactinomyces vulgaris and micropolyspora faeni, and compared with contemporary lung function parameters. in the acute phase, antibodies of several ig classes were present, those of igg and iga being most common. at the end of the follow-up, the mean values of all antibody titres were lower than ... | 1990 | 2340884 |
| detection of antigens in lung biopsies by immunoperoxidase staining in extrinsic allergic bronchiolo-alveolitis (eaba). | open lung biopsy specimens of 14 patients with extrinsic allergic bronchiolo-alveolitis (eaba) were studied by immunoperoxidase staining with avidin-biotin-complex method using nonconjugated antisera to micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, and aspergillus umbrosus. antigenic intracellular material was found in macrophages and giant cells of granulomas in the lung tissue of 8 patients. this finding supports the hypothesis which suggests that macrophages and t-l ... | 1985 | 2409726 |
| comparative 16s rrna oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives. | the phylogenetic incoherency of the genus bacillus as presently described is demonstrated by analysis of both published and new data from comparative 16s rrna cataloguing of nine bacillus species and a number of related non-bacillus taxa, i.e. caryophanon latum, filibacter limicola and planococcus citreus. while the ellipsoidal-spore-forming bacilli, e.g. b. subtilis and allied species, formed a coherent cluster, the round-spore-forming bacilli showed a higher degree of relationship to the non-s ... | 1987 | 2452227 |
| mast cells are important in the development of hypersensitivity pneumonitis. a study with mast-cell-deficient mice. | we have previously reported that c57b1/6 mice develop lung lesions similar to human hypersensitivity pneumonitis (hp) by repeated transnasal administration of thermoactinomyces vulgaris antigen. since the hp-like lesions were induced via respiratory route and by the causative antigen in human hp (farmer's lung), it seems that this murine model is useful for investigating the cell-to-cell interactions in human hp. to clarify the involvement of mast cells (mc) in the development of hp, t. vulgaris ... | 1989 | 2476490 |
| spontaneous regression in murine hypersensitivity pneumonitis: lack of immunological tolerance. | we have reported a murine model of hypersensitivity pneumonitis (hp) by transnasal administration of thermoactinomyces vulgaris (tv) antigen and shown a potential usefulness in the study of the immunopathogenesis of hp. since we observed spontaneous regression (sr) of the lung lesions with continuous administration of tv antigen in our model of hp, attempts were made to study if sr is caused by immunological tolerance. the titers of anti-tv igg antibody in serum and bronchoalveolar lavage fluid ... | 1989 | 2527204 |
| distribution of clinically important thermophilic actinomycetes in vegetable substrates and soil in north-western india. | medically important thermophilic actinomycetes were isolated from 218 (64%) of 341 samples of vegetable substrates and soil examined from sites in north-western india. thermoactinomyces vulgaris (t. candidus) was the commonest species, occurring in 56% of samples, followed by saccharomonospora viridis in 29%, thermoactinomyces thalpophilus in 27%, faenia rectivirgula (micropolyspora faeni) in 21% and thermoactinomyces sacchari in 14%. t. vulgaris and t. thalpophilus were isolated from all types ... | 1989 | 2589849 |
| comparative immunochemical analysis of five thermoactinomyces strains. | the antigen structures in mycelial extracts of 4 strains of thermoactinomyces vulgaris and 1 strain of thermoactinomyces candidus were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining. for immunological characterization, antigens were transferred electrophoretically to nitrocellulose membranes and blotted with t. vulgaris strain-specific antisera and with sera of farmer's lung patients. cross-reactions between the strains were also studied using immuno ... | 1989 | 2613347 |
| crystal structure of thermitase from thermoactinomyces vulgaris at 2.2 a resolution. | the crystal structure of thermitase from thermoactinomyces vulgaris has been determined by x-ray diffraction at 2.2 a resolution. the structure was solved by a combination of single isomorphous replacement and molecular replacement methods. the structure was refined to a conventional r factor of 0.24 using restrained least square procedures corels and prolsq. the tertiary structure of thermitase is similar to that of subtilsin bpn'. the greatest differences between these structures are related t ... | 1989 | 2647518 |
| [allergic alveolitis in agricultural workers, caused by thermophilic bacteria or fungi]. | between 1976-1986 fifty-seven patients with farmer's lung have been diagnosed in hungary on the basis of data obtained from public institutes for tuberculosis. that are 0.08% of the 744,300 manual workers employed in agriculture and forestry. in the counties of borsod and szolnok the rate was 0.5% (referred to 62,900 and 36,000 individuals, respectively). regional accumulation of the different provoking agent's and variation of disease prevalence seem to be in correlation with geographical, clim ... | 1989 | 2686222 |
| crystallographic refinement by incorporation of molecular dynamics: thermostable serine protease thermitase complexed with eglin c. | in order to investigate the principles of protein thermostability, the crystal structure of thermitase from thermoactinomyces vulgaris, a thermostable member of the subtilisin family of serine proteases, has been determined in a complex with eglin c. eglin c is a serine protease inhibitor from the leech hirudo medicinalis. after data collection with a television area-detector diffractometer and initial structure solution by molecular-replacement methods, crystallographic refinement proceeded wit ... | 1989 | 2688688 |
| farmer's lung in infants and small children. | a boy and a girl, 10 weeks and 3 years of age, respectively, were admitted to our department with low temperature, dry cough, fatigue and weight loss. in both patients pulmonary x-rays showed diffuse, bilateral, micronodular infiltrations, and sparse signs of fibrosis. serum igg and blood eosinophils were abnormally high. after a stay in hospital for 3 weeks, the patients recovered slowly. however, after a few days at home, they were readmitted with the same symptoms. family histories revealed t ... | 1989 | 2719181 |
| [the tresh dust lung syndrome in children]. | farmer's lung, caused by the inhalation of microspores--particularly of the genus, thermoactinomyces--has been recognised for the past 30-40 years as a condition affecting adult farm workers, especially up to the mid-sixties when undried crops were still threshed indoors. although it used to be extremely rare in children, between 1985 and 1989 occurred five of the seven cases reported hitherto. the possibility is suggested that with the increasingly large scale use of grain drying plant and stub ... | 1989 | 2734095 |
| aeroallergens in dairy barns near cooperstown, new york and rochester, minnesota. | we sampled atmospheric barn air using a volumetric air sampler in ten barns near cooperstown, ny and six barns near rochester, mn, and, with radioimmunoassays, measured allergens of aspergillus fumigatus, thermoactinomyces vulgaris, micropolyspori faeni, short ragweed, rye grass group i pollen, alternaria (alt-1), dermatophagoides sp. lepidoglyphus destructor, common insect allergen, mouse urine, rat urine, and cattle epithelium. the most abundant allergen present was a. fumigatus followed by l. ... | 1989 | 2764367 |
| lymphocyte activation with release of soluble mediators induced by thermoactinomyces vulgaris in vitro. | murine spleen lymphocytes stimulated in vitro with a hypersensitivity pneumonitis-associated bacterium, thermoactinomyces vulgaris, were found to secrete interleukin-2 up to 7 days after mitomycin c blockade. they exerted helper effect in secondary mitogen or antigen-induced lymphocyte proliferation. cyclosporin a, an inhibitor of interleukin-2 synthesis, caused a complete abrogation of the helper effect, suggesting that the effect was mainly due to interleukin-2. indomethacin, an inhibitor of p ... | 1989 | 2785089 |