| relationship between the quality of the inoculum material and carminomycin biosynthesis by a culture of actinomadura carminata. | the effect of the inoculum mycelium quality on carminomycin biosynthesis by actinomadura carminata was studied. the time of the organism growth on the culture medium containing cornsteep liquor continued for 6 hours without losing by the inoculum of its seeding qualities during that period. the mycelium growth in the inoculum was more intensive under conditions of moderate aeration, i.e. 0.98-2.64 mg o2h1-min. anincrease in the aeration rate up to 18.56 mg o2/1-min resulted in the growth suppres ... | 1977 | 18979 |
| mycetoma in somalia - results of a survey done from 1959 to 1964. | the pasteur institute studied 103 mycetoma patients in somalia between 1959 and 1964. grains were seen in 94 of them and this, added to cultural features, allowed the diagnosis of 60 pathogens as follows: 44 madurella mycetomi, 1 leptosphaeria senegalensis, 7 pyrenochaeta romeroi (or madurella grisea), 3 allescheria boydii, 1 fusarium sp., 3 neotestudina (zopfia) rosatii, and 1 unidentified; 34 were actinomycetes: 24 streptomyces somaliensis, 4 actinomadura madurae, 3 a. pelletieri and 3 nocardi ... | 1977 | 23657 |
| the exocellular dd-carboxypeptidase-endopeptidase of streptomyces albus g. interaction with beta-lactam antibiotics. | kinetically, the three-step model proposed for the interaction between beta-lactam antibiotics and the exocellular dd-carboxypeptidases-transpeptidases of streptomyces r61 and actinomadura r39 [frère, ghuysen & iwatsubo (1975) eur. j. biochem. 57, 343--357; fuad, frère, ghuysen, duez & iwatsubo (1976) biochem. j. 155, 623--629] applies to the interaction between the much less penicillin-sensitive exocellular dd-carboxypeptidase-endopeptidase of streptomyces albus g and at least phenoxymethylpeni ... | 1978 | 105727 |
| [new genus of actinomycetes: excellospora gen. nov]. | morphological, cultural and physiological properties of two strains of soil thermophilic actinomycetes were studied. these strains were compared to the actinomycetes of related genera and were found to be close to micropolyspora and actinomadura. however, they differed from both genera by the structure of the sporiferous apparatus, from micropolyspora by the type of the cell wall, and from actinomadura by the fatty acid composition of lipids of the mycelium. a new genus of actinomycetes, excello ... | 1975 | 125848 |
| a study of 3891 cases of mycoses in the tropics. | 4103 cases suspected of mycoses were analysed as to sex, age and site of disease and 3891 were proved cases. this group formed 50% of total mycoses or 13-93% of all dermatoses recorded in the government general hospital, madras, during the period of study. there were 66-26% adult female, 27-6% adult male and 6-14% were below 13 years. dermatophytoses were found in 73-5%; the other common diseases were tinea versicolor (17-68%) and candidiasis (12-43%). multiple sites of involvement or more than ... | 1976 | 134458 |
| [isolation of actinomadura from soils on selective media with kanamycin and rifampicin]. | | 1978 | 146454 |
| 2'-amino-2'-deoxyadenosine produced by a strain of actinomadura. | | 1979 | 317287 |
| a method for determining in-vitro drug susceptibilities of some nocardiae and actinomadurae: results with 17 antimicrobial agents. | a simple timesaving method for determining drug susceptibilities in vitro of isolates of nocardia and actinomadura is reported. an isolate is considered "susceptible" when the quantity of drug required for inhibition of growth is that concentration which might be obtained in serum by conventional therapy. sulfonamides remain the drugs of choice for treating disease due to nocardia species. although doxycycline and minocycline appear to be very effective against nocardia species, susceptibility t ... | 1977 | 327792 |
| preliminary investigation of the use of the enzyme linked immunosorbent assay (elisa) in the serodiagnosis of mycetoma. | antibody levels in a small group of sudanese patients with clinically diagnosed mycetoma, and control groups were measured by countercurrent immunoelectrophoresis (cie) and enzyme linked immunosorbent assay (elisa). antigens were prepared from the following organisms: streptomyces somaliensis, actinomadura madurae, a. pelletieri, nocardia brasiliensis and madurella mycetomi. positive reactions were obtained in clinical cases against the homologous antigens in both cie and elisa; all heterologous ... | 1978 | 360443 |
| fermentation, isolation, and biological activity of maduramycin: a new antibiotic from actinomadura rubra. | in a continuing search for new antibiotics, the species actinomadura rubra (sveshnikova et al.) j. meyer et m. sveshnikova 1974 (strain imet 13001) was found to produce a red pigment with indicator properties, designated maduramycin. the pigment (c28h22o10; m.w. 518 m/e-; m.p. 305--310 degrees c (dec.); uvmax 225.307 nm) possesses a strong antimicrobial activity against gram-positive bacteria, including strains which produce inactivating enzymes for some commercial antibiotics. maduramycin forms ... | 1978 | 362738 |
| [new elementary structures of actinomycete spores of the genera actinomadura and streptomyces]. | the structure of spore surfaces was studied by electron microscopy in five species of actinomycetes, and two new types of elementary surface structures were discovered. the surface of spores in streptomyces regensis consists of long flexible, closely interwoven, band-like structures 25 a wide. ring-like structures 140--150 a in diameter can be observed on spores of actinomadura verrucosospora; their clusters resemble grapes and the spore silhouette looks like a cone. rod-like structures of spore ... | 1979 | 423813 |
| new species of the genus actinomadura. | | 1979 | 442701 |
| [agents of mycetomas: a bacteriological and parasitological study (author's transl)]. | microscopic agents responsible of mycetomas are reviewed. they may be bacteria belonging to the actinomycetalae order (nocardia, actinomadura, streptomyces) or very various fungi belonging to the classes of adelomycetes or ascomycetes (madurella, leptosphaeria, neotestudina, etc.). though the procedures for collecting and transporting samples are the same, direct microscopic examination gives possibility to choose among the selective culture media the most appropriate ones to isolate either fung ... | 1979 | 459790 |
| numerical taxonomy of actinomadura and related actinomycetes. | one hundred and fifty-six actinomadura strains, marker strains of related taxa, and related isolates from bagasse and fodder were the subject of numerical phenetic analyses using 90 unit characters. the data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering was achieved using both single and average linkage algorithms. cluster composition was not markedly affected either by the coefficient or clustering algorithms used or by test error, esti ... | 1979 | 479835 |
| in vitro activity of cefoxitin against nocardia spp. and actinomadura spp. | | 1979 | 500519 |
| mycetomas in madras. | in 90 of 150 cases clinically diagnosed as mycetoma in the department of pathology, madras medical college, from january 1964 to june 1975, histopathological study revealed granules. besides special staining procedures, cultural methods were undertaken in recent cases. the age and sex incidence, site of lesion and the species of fungi identified on the basis of histological morphology of the granules are analysed. the disease was predominatly seen in the age group 21-40. men were more frequently ... | 1977 | 558655 |
| mycetomas in north india. | of 60 clinically suspected cases of mycetoma, 20 were confirmed by cultural and histopathological methods. the feet were found to be affected in 70% of the cases. the age group most affected by the disease was 20-40 years. men were involved in 18 while females were affected in 2 cases only. actinomycetomas (70%) were found more often than eumycetomas (30%). nocardia species were identified in 35% of cases and madurella mycetomi in 25%. there were four cases (20%) where the etiological agents cou ... | 1979 | 575236 |
| [selection of active strains of actinomadura carminata, the producer of carminomycin]. | the method of selecting active strains among a definite group of analogue-resistant mutants was used on the basis of studying the dependence of the carminomycin-producing organism growth and the antibiotic synthesis level on some metabolites. as a result, gamma-ray induced mutants 4 times more active than the parent strains were obtained. | 1978 | 623444 |
| [the role of histopathology in the diagnosis of mycetomas. two cases of maduromycosis. actinomadura pelletieri and leptosphaeria tompkinsii (author's transl)]. | | 1978 | 686856 |
| red grain mycetoma of the scalp (actinomadura pelletieri). a case report from india. | a case of red grain mycetoma on the scalp is described. the disease, caused by actinomadura pelletieri, is extremely rare in this part of the world, and from india this is perhaps the first case with scalp involvement. the lesion showed satisfactory response to partial excision followed by chemotherapy with streptomycin. | 1978 | 692630 |
| mycetomas in goats. | naturally occurring mycetomas were found in 3 goats. two had mycetomas on their hind legs and the third on its left scapula. in 2 goats the causative agents were identified by culture, histopathology and serology as actinomadura madurae. in the remaining goat diagnosis was based only on histopathology and the causative agent was considered to be a. pelletierii. despite minor differences between goat and human strains of a. madurae, it would seem that goats could be useful experimental models. | 1978 | 705558 |
| [antibiotic activity of cultures of the species of the genus actinomadura]. | sixty five cultures of actinomadura were isolated from samples of different soils. the study of their antibiotic activity on liquid nutrient media showed that 49 of them were active against gram-postive bacteria. the antibiotics isolated fromsome cultures of am. carminata, am. rubra, am. coerulea and am. luteofluorescens had antitumor effect in experiments on animals. | 1978 | 727753 |
| the exocellular dd-carboxypeptidase-endopeptidase from streptomyces albus g. purification and chemical properties. | the exocellular dd-carboxypeptidase-endopeptidase of streptomyces albus g was purified to protein homogeneity and compared with the exocellular dd-carboxypeptidases-transpeptidases of streptomyces r61 and actinomadura r39. the s. albus g enzyme, as it is isolated, occurs in two forms. enzyme i (30% of the total amount) and enzyme ii (70% of the total amount) are identical in all respects, except that, by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate, enzyme i has ... | 1978 | 743235 |
| [isolation of new species of the genus actinomadura on selective media with rubomycin]. | in the screening programme of organisms producing new antibiotics on selective media with rubomycin (5, 10, 20 gamma/ml) 4 unusual actinomyceteus cultures forming short, straight, hook-like and monospiral spore chains on the aerial mycelium were isolated. the study of the composition of the cell walls showed that they contained meso-diaminopimelic acid and madurose, a differentiating sugar. on the basis of the morphological structure and composition of the cell walls the isolates were classified ... | 1975 | 773288 |
| distribution of menaquinones in actinomycetes and corynebacteria. | menaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined. dihydromenaquinones having nine isoprene units were the main components isolated from gordona, mycobacterium, corynebacterium bovis, corynebacterium glutamicum and a strain labelled nocardia farcinica, but dihydromenaquinones having eight isoprene units were characteristic of other corynebacterium species and representatives of the 'rhodochrous' complex. tetrahydromenaquinones having six ... | 1977 | 894261 |
| [foot mycetoma of double etiology]. | a patient coming from an area of dry tropical forest was studied. this patient had in his right foot two tumor-like growths localized, one in the anterior and superior part of the foot, the other near the malleoli. from the first tumor, light-colored grains were obtained, and actinomadura madurae was cultured. from the tumor near the malleoli only black grains were isolated from which a pyrenochaeta sp. was cultured. | 1977 | 905922 |
| [electron microscopic study of spore formation in actinomadura verrucosospora]. | the formation of spores was studied with a morphologic type of the actinomadura genus, acd. verrucosospora (nonomura a. ohara, 1971. spore formation begins with the division of a hypha by cross-partitions which, as a rule, appear as monolayered on cross-sections, thus differing from sporulation septae found in other actinomycetes. the structure and shape of septae change in the course of spore formation: first, they consist of one layer and have the same thickness as the cell wall; later, they c ... | 1977 | 909469 |
| [new species of actinomadura coeruleoviolacea sp. nov. and its antagonistic properties]. | eight strains of actinomycetes isolated from soil samples were described as representatives of actinomadura coeruleoviolacea sp.nov. the species was characterized by straight and sometimes branching short chains of spores, smooth spores, blue aerial mycellium and violet soluble pigment. all the strains showed antibacterial activity when cultivated in liquid media. the pigment differed from all known pigments. it is probably a new natural compound. | 1976 | 999259 |
| [subsequent mutagenesis under the action of uv rays in a culture of actinomadura carminata, a producer of carminomycin]. | the succession of some markers on the chromosomes of actinomadura carminata was shown with the method of successive mutagenesis based on the preferable effect of the mutagen on dna in the site of replication. the spore germination was synchronized by selection of the spores of the definite size and maintenance at a temperature of 0 degrees. the periods of formation of the highest numbers of morphological and auxotrophic mutants were compared with the time of dna replication. | 1976 | 999262 |
| [effect of aeration conditions on the biosynthesis of carminomycin by an actinomadura carminata culture]. | the effect of the aeration rate on biosynthesis of carminomycin by actinomadura carminata and biochemical changes in the fermentation broth on the use of 3 complex media of different composition was studied. the carminomycin-producing organism can grow and produce the antibiotic within the ranges of the aeration changes from 0.98 to 18.56 mgo2/1-min. a decrease in the maximum rate of oxygen dissolution up to 0.98 mgo2/1-min resulted in some decrease in the activity level. intensive aeration, i.e ... | 1976 | 1020937 |
| mycetoma in iran. | the subject of mycetoma is reviewed and 7 cases are reported for the first time from southern iran. four were causes by actinomyces israelii and one each by nocardia asteroides, actinomadura madurae and allescheria boydii. | 1975 | 1092627 |
| a novel actinomycete from sugar-cane bagasse: saccharopolyspora hirsuta gen. et. sp. nov. | a new species of nocardioform actinomycete isolated from spontaneously heated sugar-cane bagasse is described as saccharopolyspora hirsuta gen. et sp. nov. it has affinities with species of both nocardia and actinomadura but can be distinguished from both genera by its morphology, sporulation, wall and lipid analyses, antibiotic resistance, degradation and carbon utilzation tests. | 1975 | 1097584 |
| [the application of chorioallantois membranes of chicken embryos for the virulence determination of nocardia asteroides (author's transl)]. | the pathogenicity of nocardia asteroides and other strains of different nocardia species against chicken embryos was investigated. the strains were inoculated on the chorioallantois membrane of embryonated eggs and the resulting lesions were judged. 55 nocardia asteroides strains and some strains of the species n. brasiliensis, n. leishmanii, n. caviae, n. (actinomadura) madurae, n. caprae, n. rubra, n. pellegrini, n. corallina and n. polychromogenes were studied. 47 strains of the species n. as ... | 1975 | 1101585 |
| [systematic position of streptomyces luteofluorescens]. | on the basis of the cell wall composition (the presence of meso-diaminopimelic acid and madurose sugar) and according to the morphological structure, streptomyces luteofluorescens (type culture 719, isp 5398) is transferred to actinomadura genus. the organism is considered to be a new species of the actinomadura genus -- actinomadura luteofluorescens (shinobu, 1962) preobrazhenskaya et lavrova, 1974, comb. nov. | 1975 | 1160657 |
| production of carminomycin by auxotrophic mutants of actinomadura carminata. | | 1975 | 1189475 |
| [ultrastructure of the mycelium and spores of actinomadura fastidiosa sp. nov]. | a new species of the actinomadura genus, a. fastidiosa sp. nov., is described. the ultrastructure of the vegetative mycelium and spores of this organism was studied. the vegetative cells have a multilayered cell wall, often consisting of five layers with different thickness and electron density. the spores are similar to the vegetative cells by their inner structure but have a thicker wall. | 1975 | 1207505 |
| [actinomadura cremea var. rifamycini, a producer of rifamycin o]. | | 1975 | 1225170 |
| [optimization of the nutrient medium makeup for the biosynthesis of carminomycin using mathematical methods of experimental design]. | the nutrient medium for biosynthesis of carminomycin by actinomadura carminata, strain 4281 was optimized with the methods of mathematical planning of the experiment. the starting variant of the medium, production of carminomycin on which was assumed equal to 100 per cent was enriched during the experiments carried out according to the patterns of the orthogonal latin squares. the production levels of carminomycin on the above medium was more than 3 times higher than those on the starting medium ... | 1975 | 1225180 |
| [isolation of new species of the genus actinomadura on selective media with streptomycin and bruneomycin]. | in the screening programme using selective media with streptomycin (25, 50 and 100 gamma/ml) and bruneomycin (0.5, 1 and 2 gamma/ml) 3 unusual actinomycetous cultures forming short, rarely long chains of straight, hook-like or spiral spores with 1 or rarely 2 turns were isolated. the study of the cell wall composition of the isolates showed that the walls contained meso-diaminopimelic acid and a sugar madurose. on the basis of the morphological structure and the cell wall composition the isolate ... | 1975 | 1225188 |
| [heterogeneity of the genus actinomadura lechevalier a. lechevalier]. | studies of the morphology of actinomadura spp. have shown that the genus comprises several morphological types of actinomycetes which differ by the presence and structure of the sporophores on the aerial and substrate mycelium. the actinomycetes differ also by the fatty acid composition of lipids in the mycelium, and can be subdivided into three groups: (1) fatty acids with a straight chain prevail; (2) fatty acids with branched chains of the iso- and anteiso structure predominate; and (3) fatty ... | 1975 | 1226138 |
| [epidemiology of mycetoma in mexico: study of 2105 cases]. | a survey was carried out in mexico to determine the incidence and epidemiological characteristics of mycetoma. data was collected from a total of 2105 cases of mycetoma throughout a 30 year period (1956-1985), with an average incidence of 70 cases per year. results showed a sex distribution of 76.1% male and 23.9% females. age distribution indicated a 35% between 16 to 30 and 23% between 31 to 40 year old population. most cases occurred in land-workers (60.2%) and in housewives with rural reside ... | 1992 | 1308000 |
| an inhibitor for inositol-specific phospholipase c from actinomadura sp. | | 1992 | 1328124 |
| [mycetoma due to actinomadura madurae. a report of 2 cases]. | actinomadura madurae mycetoma was diagnosed in two patients by the dermatologic outpatient clinic of the clementino fraga filho university hospital (ufrj) and the antonio pedro university hospital (uff). the first case was a 27-year-old negro male from the outskirts of rio de janeiro, who was injured prior to the onset of the disease in 1988. the affected left foot showed swelling, nodules, sinus tracts, purulent discharge containing grains, and serious bone involvement with lytic lesions. histo ... | 1992 | 1342096 |
| matlystatins, new inhibitors of typeiv collagenases from actinomadura atramentaria. ii. biological activities. | matlystatin a, the main component of matlystatins, inhibits 92 kda and 72 kda typeiv collagenases with ic50 values of 0.3 microm and 0.56 microm, respectively, while 7- to 11-fold greater concentrations are required to inhibit thermolysin and aminopeptidase m. the inhibition is reversible and competitive with respect to gelatin. it inhibits the invasion of basement membrane matrigel by human fibrosarcoma ht1080 dose-dependently with an ic50 value of 21.6 microm. | 1992 | 1361486 |
| cp-82,996, a novel diglycoside polyether antibiotic related to monensin and produced by actinomadura sp. | a new polyether antibiotic cp-82,996 (c50h86o16) was isolated by solvent extraction from the fermentation broth of actinomadura sp. (atcc 53764). following purification by silica gel column chromatography and crystallization, the structure of cp-82,996 was determined by a single crystal x-ray analysis. the structure is closely related to monensin, but is unique in that it contains two sugar groups, whereas monensin has none. the 1h and 13c nmr chemical shifts and assignments for cp-82,996 were e ... | 1990 | 1366920 |
| primary and predicted secondary structure of the actinomadura r39 extracellular dd-peptidase, a penicillin-binding protein (pbp) related to the escherichia coli pbp4. | | 1992 | 1417760 |
| the isolation and structure of cp-120,509, a new polyether antibiotic related to semduramicin and produced by mutants of actinomadura roseorufa. | | 1992 | 1429242 |
| matlystatins, new inhibitors of typeiv collagenases from actinomadura atramentaria. i. taxonomy, fermentation, isolation, and physico-chemical properties of matlystatin-group compounds. | during the course of a screening for inhibitors of typeiv collagenases, new metabolites, designated matlystatins, have been isolated from an actinomycete strain, which was identified as a strain of actinomadura atramentaria. matlystatins were composed of five congeners, which were separated and purified by n-butanol extraction and chromatography. | 1992 | 1468979 |
| new hydroxybenanomicins produced by actinomadura. | a soil microorganism, actinomadura sp. mh193-16f4, produces an antifungal antibiotic benanomicin a and several related compounds. among them, benanomicin a is the best candidate as a chemotherapeutic agent in terms of antifungal activity, toxicity and water-solubility. three novel hydroxyl congeners, 3'-hydroxybenanomicin a, 7-hydroxybenanomicin a and 7-hydroxybenanomicinone have been isolated from the culture broth of the mh193-16f4 strain or its mutant. interestingly, 3'-hydroxy-benanomicin a ... | 1992 | 1478160 |
| determination of the acyl moieties of the antibiotic complex a40926 and their relation with the membrane lipids of the producer strain. | structures of the fatty acid residues characterizing the various components of a40926 were determined by gas chromatography/mass spectrometry on the methyl esters obtained by methanolysis of the complex. the results confirm the residues previously assigned to factor a (n-undecanoic acid) and b (10-methyl-undecanoic acid) and establish the residues of factor a1 (9-methyl-decanoic acid), b1 (n-dodecanoic acid), rs1 (8-methyl-nonanoic acid), rs2 (n-decanoic acid), and rs3 (n-tridecanoic acid). as t ... | 1992 | 1504339 |
| enhancement of secondary metabolite production using the antibiotic gradient-plate technique. | we have used the antibiotic gradient-plate technique to generate antifungal producing actinomadura strains that synthesize enhanced quantities of component 3a of a macrocyclic lactam antifungal compound sch 38516. seventy-nine colonies were selected from a mixture of rifampicin and spectinomycin gradient plates. the two fermentation extracts described produced an enhanced 3a component (identified with silica gel tlc, hplc, and bioautography) when compared to the parent culture isolate extracts ( ... | 1992 | 1531080 |
| primary and predicted secondary structures of the actinomadura r39 extracellular dd-peptidase, a penicillin-binding protein (pbp) related to the escherichia coli pbp4. | as derived from gene cloning and sequencing, the 489-amino-acid dd-peptidase/penicillin-binding protein (pbp) produced by actinomadura r39 has a primary structure very similar to that of the escherichia coli pbp4 [mottl, terpstra & keck (1991) fems microbiol. lett. 78, 213-220]. hydrophobic-cluster analysis of the two proteins shows that, providing that a large 174-amino-acid stretch is excluded from the analysis, the bulk of the two polypeptide chains possesses homologues of the active-site mot ... | 1992 | 1554361 |
| nonmycetomic actinomadura madurae infection in a patient with aids. | actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. it has not previously been reported to have caused invasive pulmonary or disseminated infection in humans. we describe an aids patient with opportunistic a. madurae-induced pneumonia and bacteremia. the isolate from the patient's blood was subjected to dilutional antimicrobial susceptibility tests with 12 antimicrobial agents and was found to have a wide spectrum of susceptibili ... | 1992 | 1572956 |
| cp-82,009, a potent polyether anticoccidial related to septamycin and produced by actinomadura sp. | a new polyether antibiotic cp-82,009 (c49h84o17) was isolated by solvent extraction from the fermentation broth of actinomadura sp. (atcc 53676). following purification by column chromatography and crystallization, the structure of cp-82,009 was elucidated by spectroscopic (nmr and ms) methods. the absolute stereochemistry was determined by a single crystal x-ray analysis of the corresponding rubidium salt. cp-82,009 is among the most potent anticoccidial agents known, effectively controlling th ... | 1992 | 1577662 |
| polyacrylamide gel electrophoresis analysis of ribosomal protein: a new approach for actinomycete taxonomy. | the ribosomal (r)-proteins from eleven streptomyces strains representing various numerical taxonomic clusters were compared by two-dimensional polyacrylamide-gel electrophoresis (2d-page). the protein patterns were specific for each species. an attempt was made to identify one strain of streptomyces by both traditional taxonomic methods and 2d-page analysis of the r-protein patterns. both methods identified the strain as streptomyces lavendulae, and protein pattern analysis also showed that s. g ... | 1992 | 1612446 |
| macrolactams: a novel class of antifungal antibiotics produced by actinomadura spp. scc 1776 and scc 1777. | three novel antifungal antibiotics, sch 38518, sch 39185 and sch 38516 were isolated from the fermentation broths of two actinomycetes identified by chemical, morphological and physiological analysis as a new species of actinomadura. the compounds were isolated from broth by solvent extraction and purified by silica gel chromatography. physico-chemical properties, mass spectral analysis, ir and uv suggested the compounds were similar. sch 38518 and sch 39185 have a molecular formula of c25h48n2o ... | 1992 | 1624364 |
| macrolactams: two novel homologous series of compounds produced by actinomadura sp. scc 1778. | eight antifungal compounds were identified from the fermentation of actinomadura sp. scc 1778. this culture produces four homologous compounds (c22h42n2o5 approximately c25h48n2o5) containing the sugar, mycosamine, and four homologous compounds (c22h42n2o5 approximately c25h48n2o5) containing the sugar, 3,6-dideoxy-3-amino-l-talopyranose. five of the compounds identified were novel macrolactams. all these compounds exhibit antifungal activity against candida spp. with geometric mean mics ranging ... | 1992 | 1624365 |
| anomalous behaviour of a protein during sds/page corrected by chemical modification of carboxylic groups. | the 29,000-mr actinomadura r39 beta-lactamase exhibited a remarkably low electrophoretic mobility on sds/page, yielding an mr value almost twice that computed from the corresponding gene sequence. we showed that chemical modification of the carboxylic groups of glutamic acid and aspartic acid residues restored a normal electrophoretic mobility and that the anomalous behaviour of that protein on sds/page was due to its very large negative charge at neutral ph. we also compared the behaviour of th ... | 1991 | 1747131 |
| actinomadura madurae causing mycetomas in madras. | two hundred and ten histologically diagnosed cases of mycetoma filed in the departments of pathology of four medical colleges in tamil nadu, india, between 1964-1987 were subjected to detailed histopathological study; actinomadura madurae, was found in 73 of them. in nine cases of mycetoma pedis, the organism a. madurae has been isolated in pure culture. in one case, the lesions were present in both the feet. the characteristic cream-coloured granules were seen in all. the lesions responded well ... | 1991 | 1752637 |
| cp-91,243 and cp-91,244, novel diglycoside polyether antibiotics related to uk-58,852 and produced by mutants of actinomadura roseorufa. | | 1991 | 1842675 |
| polyacrylamide gel electrophoresis analysis of ribosomal protein at-l30 as a novel approach to actinomycete taxonomy: application to the genera actinomadura and microtetraspora. | actinomycete ribosomal protein at-l30 exhibits electrophoretic mobility that is specific for each genus. on the basis of this fact, we analyzed ribosomal at-l30 proteins from 26 type strains of species belonging to the genera actinomadura and microtetraspora. the electrophoretic mobilities of at-l30 preparations from these strains, as determined by two-dimensional polyacrylamide gel electrophoresis, revealed that they could be divided into two groups, one group with relative electrophoretic mobi ... | 1991 | 1854638 |
| production and properties of xylanases from thermophilic actinomycetes. | 30 strains of xylanolytic thermophilic actinomycetes were isolated from composted grass and cattle manure and identified as members of the genera thermomonospora, saccharomonospora, microbispora, streptomyces and actinomadura. screening of these strains for extracellular xylanase indicated that strains of saccharomonospora and microbispora generally were poor xylanase producers (0.5-1.5 u/ml) whereas relatively high activities were observed in cultures of streptomyces and actinomadura (4-12 u/ml ... | 1991 | 1905513 |
| fluvirucins a1, a2, b1, b2, b3, b4 and b5, new antibiotics active against influenza a virus. iv. taxonomy on the producing organisms. | the morphology, chemotaxonomy, and cultural and physiological characteristics were examined on the five strains of actinomycetes which produce antiviral antibiotics, fluvirucin congeners. all strains have meso-2,6-diaminopimelic acid in the cell wall. four strains, q464-31, l407-5, r359-5 and r516-16, belong to the maduromycetes since they have madurose in the whole cell. the remaining one strain, r869-90, has rhamnose but no madurose, and is a nocardioform actinomycete. these five strains were ... | 1991 | 1938616 |
| ragged n-termini and other variants of class a beta-lactamases analysed by chromatofocusing. | four beta-lactamases excreted by gram-positive bacteria exhibited microheterogeneity when analysed by chromatofocusing or ion-exchange chromatography. ragged n-termini were in part responsible for the charge variants, but deamidation of an asparagine residue was also involved, at least for the bacillus licheniformis enzyme. the activity of a contaminating proteinase could also be demonstrated in the case of actinomadura r39 beta-lactamase. with that enzyme, proteolysis resulted in partial inacti ... | 1991 | 1996951 |
| isolation of new minor benanomicins. | four minor benanomicins, dexylosylbenanomicins a and b, 2'-demethylbenanomicin a and 7-methoxybenanomicinone have been isolated from the culture filtrate of actinomadura sp. mh193-16f4. their structures were confirmed by spectral analyses. dexylosylbenanomicins a and b were derived chemically from benanomicins a and b, respectively. | 1991 | 2010353 |
| maduropeptin, a complex of new macromolecular antitumor antibiotics. | maduropeptin, a complex of new macromolecular antitumor antibiotics, is a metabolite of actinomadura madurae h710-49. the active components maduropeptins a1, a2 and b are acidic chromopeptides with mw of around 22,500 and composed of 14 types of amino acids and an unstable chromophore. the antibiotics are active in vitro against gram-positive bacteria and highly cytotoxic to tumor cells. they produced significant prolongation of survival time of mice implanted with p388 leukemia and b16 melanoma ... | 1991 | 2032949 |
| [actinomadura species as antibiotic producers]. | screening of antibiotic-producing cultures among actinomadura showed that definite species mainly produced antibiotics of the same groups. thus, carminomycins were produced by all the 4 studied strains of a. carminata, maduramycins were produced by 3 strains of a. rubra, prodigiozines were produced by 3 strains of a. madurae and luzopeptines were produced by 6 strains of a. recticatena. supposedly, new antibiotics with original spectral characteristics were isolated from 2 strains of a. fulvesce ... | 1991 | 2053819 |
| structural features of cell wall teichoic acid and peptidoglycan of actinomadura cremea ina 292. | the teichoic acid from the cell wall of actinomadura cremea ina 292 has an unusual structure, being a poly(galactosylglycerol phosphate) chain with glycerol phosphate groups. monomeric units of 1-o, beta-d-galactopyranosylglycerol monophosphate are joined in the polymer by phosphodiester links involving the glycerol c3 and the galactose c6 atoms. approximately every second galactosyl substituent has a glycerol phosphate residue at its c3 atom. the teichoic acid structure was established by chemi ... | 1991 | 2070791 |
| actinomadura madurae mycetomas. | actinomycetomas are localised, chronic, suppurative and progressive infections of the skin, subcutaneous tissues and bones caused by aerobic actinomycetes. they are characterised by subcutaneous granulomas and abscesses with induration, formation of sinus tracts and production of granules which are the microcolonies of the causative agents. | 1990 | 2073207 |
| comparison of antigens from agents of actinomycetoma by immunodiffusion and electrophoresis procedures. | antigenic extracts were prepared from culture filtrates of the principal agents known to cause actinomycetoma, namely actinomadura madurae, actinomadura pelletieri, nocardia asteroides, nocardia brasiliensis, nocardia otitidis-caviarum, and streptomyces somaliensis. these antigenic preparations were compared by immunodiffusion (id), counterimmunoelectrophoresis (cie), line immunoelectrophoresis (lie) and rocket line immunoelectrophoresis (rlie), with rabbit antisera prepared against each of the ... | 1990 | 2126557 |
| red grain mycetoma of the scalp due to actinomadura pelletieri in madurai. | | 1990 | 2132513 |
| [isolation of actinomycetales from the soil of kazakhstan on selective media with antibiotics]. | about 3000 actinomycetes were isolated from various soil samples collected in 11 regions of kazakhstan. 62.7 per cent of them proved to be antagonists. for isolation of the strains, selective media supplemented with antibiotics were used. kanamycin promoted growth of actinomadura and nocardia. rubomycin promoted growth of actinomadura. tavromycetin and roseofungin were used as selective agents for the first time. tavromycetin favoured isolation of actinomadura and nocardia. roseofungin favoured ... | 1990 | 2140036 |
| pradimicins a, b and c: new antifungal antibiotics. ii. in vitro and in vivo biological activities. | pradimicins a, b and c specify novel antibiotics produced by actinomadura hibisca no. p157-2 (atcc 53557) possessing potent and broad antifungal activity in vivo. they showed moderate in vitro antifungal activity against a wide variety of fungi and yeasts including clinically important pathogens, and were highly effective in systemic infection with candida albicans in mice after iv and im administrations. pradimicin a showed in vivo therapeutic activity against c. albicans, cryptococcus neoforma ... | 1990 | 2167304 |
| mm 46115, a new antiviral antibiotic from actinomadura pelletieri. characteristics of the producing cultures, fermentation, isolation, physico-chemical and biological properties. | six strains of actinomadura pelletieri were shown to produce a novel macrolide antibiotic, designated mm 46115. mm 46115 was active against parainfluenza virus 1 and 2 and gram-positive bacteria. methods are described for the production of mm 46115 by strain ip 729.63, and for the isolation of the compound from a butanol extract of the culture filtrate. | 1990 | 2177052 |
| simaomicin (ll-d42067), a novel antibiotic from actinomadura madurae. i. taxonomy, fermentation and biological activity. | a new antibacterial antibiotic, designated simaomicin alpha (ll-d42067 alpha) was isolated from the fermentation broth of an actinomycete strain. based on cultural, physiological, morphological and chemical characteristics, culture ll-d42067 was identified as a new subspecies of actinomadura madurae. simaomicin alpha demonstrated potent activity against gram-positive bacteria and was active in vivo against a variety of eimeria species causing coccidiosis in chickens. | 1990 | 2211367 |
| actinomadura fibrosa sp. nov. isolated from soil. | a new species of the genus actinomadura which belongs to the actinomadura madurae group of goodfellow et al. was isolated from soil collected in togo, west africa. traditional taxonomic methods plus contemporary fatty acid analysis techniques were used to establish the position of this species. both physiological characteristics and fatty acid composition differentiate this strain from previously described species. this culture produces a new polyether antibiotic. it is characterized by the prod ... | 1990 | 2223595 |
| peritonitis caused by actinomadura madurae in a patient on capd. | | 1990 | 2226502 |
| comparison of species distribution and antimicrobial susceptibility of aerobic actinomycetes from clinical specimens. | to compare the species distribution and antimicrobial susceptibility of aerobic actinomycetes, we evaluated 366 isolates referred to the centers for disease control from october 1985 through february 1988. we used conventional biochemical tests to identify the various species. four species accounted for 191 (52%) of aerobic actinomycete isolates: nocardia asteroides (98 isolates), actinomadura madurae (42 isolates), streptomyces griseus (28 isolates), and nocardia brasiliensis (23 isolates). spu ... | 1990 | 2237117 |
| new antifungal antibiotics pradimicins fa-1 and fa-2: d-serine analogs of pradimicins a and c. | pradimicin fa-1 was produced via directed biosynthesis with substitution of d-serine for d-alanine in the 15-position of pradimicin a. this substitution was achieved by the addition of d-serine to the culture medium of actinomadura hibisca p157-2. likewise, pradimicin fa-2 was co-produced along with pradimicin fa-1 when the pradimicins a and c producing strain, a. hibisca a2493 was grown in d-serine-supplemented medium. the new pradimicin analogs share a common core structure of 5,6-dihydrobenzo ... | 1990 | 2258322 |
| pradimicins m, n, o and p, new dihydrobenzo[a]naphthacenequinones produced by blocked mutants of actinomadura hibisca p157-2. | four blocked mutants which accumulated new dihydrobenzo[a]naphthacenequinone metabolites, designated pradimicins m, n, o and p, have been isolated from cultures of mutants of actinomadura hibisca p157-2 resulting from treatment with n-methyl-n'-nitro-n-nitrosoguanidine. the structures of the four compounds were determined by spectral analysis. pradimicins n, o and p contain d-alanine, while pradimicin m does not. the conformations at c-5 and c-6 of these compounds are different from those of the ... | 1990 | 2272913 |
| a taxonomic review of the genus microbispora and a proposal to transfer two species to the genus actinomadura and to combine ten species into microbispora rosea. | we conducted a taxonomic review of the genus microbispora using chemotaxonomic and dna-dna hybridization techniques, and reached the following conclusions: microbispora viridis should be transferred to the genus actinomadura as actinomadura rugatobispora comb. nov., nom. nov. (type strain sf2240 = ifo 14382 = jcm 3366) and microbispora echinospora should be transferred to the genus actinomadura as actinomadura echinospora comb. nov. (type strain jcm 3148 = atcc 27300). we also propose that micro ... | 1990 | 2283505 |
| [a particularly extensive form of actinomadura madurae mycetoma]. | | 1990 | 2288032 |
| two novel phenolics from actinomadura verrucosospora. | | 1990 | 2307621 |
| sf2487, a new polyether antibiotic produced by actinomadura. | a new antibiotic sf2487 has been isolated from the culture broth of actinomadura sp. sf2487. the structure of antibiotic sf2487 was determined by spectroscopic analyses of the sodium salt and x-ray diffraction analysis of the silver salt. the antibiotic represents a new member of polyether group antibiotics known as the acyltetronic acid type 4. the antibiotic is weakly active against gram-positive bacteria and exhibits antiviral activity against influenza virus in vitro. | 1990 | 2324011 |
| experimental antitumor activity of bmy-28175 a new fermentation derived antitumor agent. | bmy-28175 is a novel antitumor antibiotic produced in fermentation by actinomadura verrucosospora. the cytotoxic effects of bmy-28175 were determined using murine and human tumor cell lines in vitro. following 72 hour exposure, the drug had ic50 values 1.5 to 13.5 ng/ml in a microtiter assay. bmy-28175 was evaluated for antitumor activity against several experimental murine and human tumor models. the drug administered ip was active against ip implanted p388 leukemia, l1210 leukemia, b16 melanom ... | 1990 | 2345072 |
| cp-84,657, a potent polyether anticoccidial related to portmicin and produced by actinomadura sp. | a new polyether antibiotic cp-84,657 (c45h78o14) was isolated by solvent extraction from the fermentation broth of actinomadura sp. (atcc 53708). following purification by column chromatography and crystallization, the structure of cp-84,657 was elucidated by spectroscopic (nmr and ms) methods. the absolute stereochemistry was determined by a single crystal x-ray analysis of the corresponding rubidium salt. cp-84,657 is among the most potent anticoccidal agents known, effectively controlling the ... | 1990 | 2380113 |
| pradimicins a, b and c: new antifungal antibiotics. i. taxonomy, production, isolation and physico-chemical properties. | new antifungal antibiotics, pradimicins a, b and c were isolated from the culture broth of actinomycete strains proposed as actinomadura hibisca. they are orange to red pigments containing a benzo[a]naphthacenequinone chromophore substituted with a d-alanine, an aminosugar and a d-xylose (pradimicins a and c). | 1990 | 2387769 |
| new antifungal antibiotics, pradimicins d and e. glycine analogs of pradimicins a and c. | new antifungal antibiotics pradimicins d and e were isolated from the culture filtrates of actinomadura hibisca p157-2 (atcc 53557) and its mutant a2660 (atcc 53762). the structure of pradimicin d is n-[[(5s,6s)-5-o-[4,6-dideoxy-4-(methylamino)-3-o-(beta-d- xylopyranosyl)-beta-d-galactopyranosyl]-5,6,8,13-tetrahydro-1,6,9,14- tetrahydroxy-11-methoxy-3-methyl-8,13-dioxobenzo[a]naphthacen++ +-2-yl] carbonyl]glycine, based on spectral analyses compared to pradimicin a. pradimicin e is the des-n-met ... | 1990 | 2387770 |
| nucleotide sequence of the gene encoding the active-site serine beta-lactamase from actinomadura r39. | the gene encoding the extracellular, active-site serine beta-lactamase of actinomadura r39, previously cloned into streptomyces lividans, has the information for the synthesis of a 304 amino acid protein, the amino terminal region of which has the characteristic features of a signal peptide. the actinomadura r39 beta-lactamase is another member of the class a beta-lactamases. in particular, it shows high homology with the beta-lactamase of bacillus licheniformis. | 1989 | 2515104 |
| at2433-a1, at2433-a2, at2433-b1, and at2433-b2 novel antitumor antibiotic compounds produced by actinomadura melliaura. taxonomy, fermentation, isolation and biological properties. | compounds at2433-a1 (a1), at2433-a2 (a2), at2433-b1 (b1), and at2433-b2 (b2) were isolated from the cultured broth of actinomadura melliaura sp. nov. (scc 1655). structurally these materials are closely related to rebeccamycin (1), an indolocarbazole antitumor antibiotic. a1, a2, b1, and b2 were active against staphylococcus aureus a9537, streptococcus faecalis a20688, streptococcus faecium (atcc 9790), micrococcus lutea (atcc 9341), bacillus subtilis (atcc 6633). a1 and b1 were active against p ... | 1989 | 2584136 |
| microbial conversion of anthracyclinones to carminomycins by a blocked mutant of actinomadura roseoviolacea. | new anthracycline antibiotics, 1-hydroxy-11-deoxycarminomycin ii and 11-deoxycarminomycin ii were produced by a blocked mutant muw1 of actinomadura roseoviolacea from epsilon-pyrromycinone and aklavinone, respectively. we found that the enzyme catalyzing hydroxylation at the c-11 position was not lost but was down regulated in the strain muw1. | 1989 | 2584152 |
| cloning and amplified expression in streptomyces lividans of the gene encoding the extracellular beta-lactamase of actinomadura r39. | by using the promoter-probe plasmid pij424, genomic dna fragments of actinomadura r39 were shown to have promoter activity in streptomyces lividans. the same 100-200-copy-number plasmid was used to clone in s. lividans tk24, the gene that encodes the actinomadura r39 beta-lactamase. gene cloning resulted in an amplified expression of the beta-lactamase when compared with the amounts of enzyme produced by the original strain (1 mg versus 0.008 mg.litre of culture-1). | 1989 | 2590171 |
| at2433-a1, at2433-a2, at2433-b1 and at2433-b2 novel antitumor compounds produced by actinomadura melliaura. ii. structure determination. | the chemical structures of at2433-a1 (2), at2433-a2 (3), at2433-b1 (4) and at2433-b2 (5) were elucidated by degradation and spectroscopic studies. compounds 2-5 are disaccharides closely related to rebeccamycin. the aglycone in 2 and 3 was determined to be 6-n-methyl-11-chloro-5h-indolo[2,3-a]pyrolo[3,4-c]carbazole and in 4 and 5 it was determined to be 6-n-methyl-5h-indolo[2,3-a]pyrolo[3,4-c]carbazole. compounds 2 and 4 are 4-n"-methyl analogs of 3 and 5. | 1989 | 2621161 |
| [antibiotics formed by actinomadura fulvescens]. | novel antibiotics with in vitro activity against gram-positive bacteria were isolated from actinomadura fulvescens ina 3321 and ina 3852. conditions for biosynthesis and isolation of antibiotics 3321 and 3852, as well as their physicochemical and biological properties were studied. chromatographic analysis of the antibiotics revealed that each of them contained two biologically active components. the components were separated with preparative chromatography. physicochemical properties of the com ... | 1989 | 2629648 |
| somnogenic activity of o-acetylated and dimeric muramyl peptides. | slow-wave sleep-promoting factors in brain and urine were identified as muramyl peptides (mps), the building blocks of bacterial cell wall peptidoglycan. in this study, structural variations of mps that occur naturally in bacterial peptidoglycan were investigated for somnogenic activity. monomeric and dimeric mps were isolated and purified from neisseria gonorrhoeae and actinomadura sp. strain r39. the structures of these mps were verified by fast atom bombardment mass spectroscopy and tandem ma ... | 1989 | 2759708 |
| antihypertensive compounds with potent protein kinases inhibitory activity. | a new indorocarbazole antibiotic sf2370 has been found in the culture broth of actinomadura sp.; it has also been found to have the activities of antihypertension and diuresis. derivatives of sf2370, na0344, na0345 and na0346, when injected (0.1-1 mg/kg i.v.) significantly lowered the blood pressure of anesthetized normotensive rats and showed a long lasting antihypertensive action. in the case of p.o. administration to conscious restrained shrs, measurement of blood pressure by the plethysmogra ... | 1989 | 2765030 |
| stereospecific 2'-amination and 2'-chlorination of adenosine by actinomadura in the biosynthesis of 2'-amino-2'-deoxyadenosine and 2'-chloro-2'-deoxycoformycin. | 2'-amino-2'-deoxyadenosine and 2'-chloro-2'-deoxycoformycin (2'-cldcf) are two nucleoside antibiotics produced by actinomadura. the biosynthesis of these two nucleoside antibiotics has been studied by the addition of [u-14c]adenosine with or without unlabeled adenine to cultures of actinomadura. by this experimental approach, it is possible to demonstrate that adenosine is the direct precursor for the biosynthesis of 2'-amino-2'-deoxyadenosine and 2'-cldcf. these conclusions are based on the obs ... | 1989 | 2784655 |
| [studies on actinomycete flora and resources in the plateau lakes in yunnan]. | during the period of 1983 to 1987 mud and water samples were collected from 12 larger lakes on the yunnan plateau. the actinomycetes in both sediment and water samples were investigated. strains producing metabolites were screened by various procedures. the results are as follow: 1. the number and composition of actinomycetes in a lake were found to have relation to the physical and chemical features of the water-body. on the basis of the number and generic diversity of actinomycetes, lakes were ... | 1989 | 2800541 |
| a40926, a new glycopeptide antibiotic with anti-neisseria activity. | in the course of a search for glycopeptide antibiotics having novel biological properties, we isolated a40926. produced by an actinomycete of the genus actinomadura, a40926 is a complex of four main factors which contain a fatty acid as part of a glycolipid attached to the peptide backbone. its activity was, in most respects, similar to that of other glycopeptides, such as vancomycin and teicoplanin. however, in addition to inhibiting gram-positive bacteria, a40926 was very active against neisse ... | 1987 | 2964225 |
| k-4, a novel inhibitor of angiotensin i converting enzyme produced by actinomadura spiculosospora. | a novel inhibitor of angiotensin i converting enzyme (ace), named k-4, was isolated from the culture broth of actinomadura spiculosospora nov. sp. k-4. the k-4 was an oligopeptide containing l-phenylalanine with (r)-1-amino-2-(4-hydroxyphenyl)ethylphosphonic acid as the c-terminal residue. the compound proved to be a specific and reversible inhibitor of ace with the inhibition constant (ki) of 0.18 microm, and inhibited ace non-competitively by use of hippuryl-l-histidyl-l-leucine (hhl) as a sub ... | 1986 | 3009378 |