| kinetic properties and related changes of molecular weight in a fructokinase from streptomyces violaceoruber. | 1. a study of the initial reaction rates at variable substrate concentrations and of the molecular weight of the enzyme in the presence of different effectors, has been carried out using fructokinase (atp: fructose 6-phosphotransferase, ec 2.7.1.4) from streptomyces violaceoruber. 2. saturation curves for mgatp or coatp are sigmoidal and they change to hyperbolic in the presence of 10 mm mg2+ or co2+ in excess over the nucleoside triphosphate. 3. saturation cuvves for fructose show intermediary ... | 1975 | 164228 |
| simultaneous loss of multiple differentiated functions in aerial mycelium-negative isolates of streptomycetes. | germination and outgrowth of spores of streptomyces alboniger, streptomyces scabies, and streptomyces violaceus-ruber in the presence of intercalating dyes resulted in a high frequency (2 to 20%) of occurrence of aerial mycelium-negative (amy-) isolates. coincident with the appearance of the amy- trait was the loss of several differentiated functions, including the characteristic pigments and earthy odor of the wild types. all s. alboniger, 27% of s. scabies, and 39% of the s. violaceus-ruber am ... | 1979 | 422514 |
| functional replacement of genes for individual polyketide synthase components in streptomyces coelicolor a3(2) by heterologous genes from a different polyketide pathway. | streptomyces coelicolor a3(2) and streptomyces violaceoruber tü22 produce the antibiotics actinorhodin and granaticin, respectively. both the aglycone of granaticin and the half-molecule of actinorhodin are derived from one acetyl coenzyme a starter unit and seven malonyl coenzyme a extender units via the polyketide pathway to produce benzoisochromane quinone moieties with identical structures (except for the stereochemistry at two chiral centers). in s. coelicolor and s. violaceoruber, the type ... | 1992 | 1400167 |
| spectroscopic studies on the metal-ion-binding sites of co2(+)-substituted d-xylose isomerase from streptomyces rubiginosus. | the coordination sphere of the two metal-binding sites/subunit of the homotetrameric d-xylose isomerase from streptomyces rubiginosus has been probed by the investigation of the co2(+)-substituted enzyme using electronic absorption, cd and magnetic circular dichroic spectroscopies in the visible region. the spectrum of the high-affinity site (b site) has an absorption coefficient, epsilon 545, of 18 m-1 cm-1, indicating a distorted octahedral complex geometry. the spectrum of the low-affinity si ... | 1990 | 2249698 |
| structure and deduced function of the granaticin-producing polyketide synthase gene cluster of streptomyces violaceoruber tü22. | a 6.5 kb region of dna from streptomyces violaceoruber, which contains polyketide synthase (pks) genes for production of the benzoisochromane quinone moiety of the antibiotic, granaticin, was cloned and sequenced. of six open reading frames (orfs) identified, four (orfs 1-4) would be transcribed in one direction and two (orfs 5 and 6) divergently from orfs 1-4. orf1 and orf2, which show evidence for translation coupling, encode (deduced) gene products which strongly resemble each other and the e ... | 1989 | 2583128 |
| single active-site histidine in d-xylose isomerase from streptomyces violaceoruber. identification by chemical derivatization and peptide mapping. | group-specific chemical modifications of d-xylose isomerase from streptomyces violaceruber indicated that complete loss of activity is fully correlated with the acylation of a single histidine. active-site protection, by the ligand combination of xylitol plus mg2+, completely blocked diethyl pyrocarbonate derivatization of this particular residue [vangrysperre, callens, kersters-hilderson & de bruyne (1988) biochem. j. 250, 153-160]. differential peptide mapping between d-xylose isomerase, which ... | 1989 | 2604694 |
| reaction of woodward's reagent k with d-xylose isomerases. modification of an active site carboxylate residue. | d-xylose isomerases from streptomyces violaceoruber, streptomyces sp., lactobacillus xylosus, lactobacillus brevis and bacillus coagulans were rapidly inactivated by woodward's reagent k. second-order rate constants in the absence of ligands, at ph 6.0 and 25 degrees c, were 41, 36, 22, 95 and 26 m-1.min-1 respectively. spectral analysis at 340 nm revealed that inactivation was correlated with modification of five, six, two, three and six carboxylate residues per monomer respectively. in the pre ... | 1989 | 2775179 |
| sequence of the ampullariella sp. strain 3876 gene coding for xylose isomerase. | the nucleotide sequence of the gene coding for xylose isomerase from ampullariella sp. strain 3876, a gram-positive bacterium, has been determined. a clone of a fragment of strain 3876 dna coding for a xylose isomerase activity was identified by its ability to complement a xylose isomerase-defective escherichia coli strain. one such complementation positive fragment, 2,922 nucleotides in length, was sequenced in its entirety. there are two open reading frames 1,182 and 1,242 nucleotides in lengt ... | 1987 | 3027039 |
| evidence for an essential histidine residue in d-xylose isomerases. | diethyl pyrocarbonate inactivated d-xylose isomerases from streptomyces violaceoruber, streptomyces sp., lactobacillus xylosus and lactobacillus brevis with second-order rate constants of 422, 417, 99 and 92 m-1.min-1 respectively (at ph 6.0 and 25 degrees c). activity was completely restored by the addition of neutral hydroxylamine, and total protection was afforded by the substrate analogue xylitol in the presence of either mg2+ or mn2+ according to the genus studied. the difference spectra of ... | 1988 | 3355509 |
| metal ion binding to d-xylose isomerase from streptomyces violaceoruber. | the binding of two activating cations, co2+ and mg2+, and of one inhibitory cation, ca2+, to d-xylose isomerase from streptomyces violaceoruber was investigated. equilibrium-dialysis and spectrometric studies revealed that the enzyme binds 2 mol of co2+/mol of monomer. difference absorption spectrometry in the u.v. and visible regions indicated that the environment of the first co2+ ion is markedly different from that of the second co2+ ion. the first co2+ appears to have a six-co-ordinate. the ... | 1988 | 3355516 |
| identification and properties of an inducible mannokinase from streptomyces violaceoruber. | | 1972 | 4635821 |
| identification and properties of an inducible and highly specific fructokinase from streptomyces violaceoruber. | | 1972 | 4635822 |
| transport of hexoses in streptomyces violaceoruber. | | 1973 | 4770791 |
| genetic homologies among streptomyces violaceoruber strains. | most of the genetic studies on streptomycetes have been done with cultures erroneously designated as streptomyces coelicolor. to determine whether these cultures are genetically homologous with the s. violaceoruber nominifer, their deoxyribonucleic acids (dna) were analyzed, and selected pairs of mutants were crossed. the four cultures used in genetic studies, and called s. coelicolor in the literature, were found to constitute a genospecies, based upon dna hybridization and recombination tests. ... | 1969 | 5370275 |
| determination of methylenomycin a synthesis by the psv1 plasmid from streptomyces violaceus-ruber sank 95570. | a plasmid (psv1) of 110 x 10(6) daltons from a methylenomycin a producing strain of streptomyces violaceus-ruber was detected on an isolated from agarose gels. elimination of this plasmid by protoplasting and regeneration resulted in the simultaneous loss of methylenomycin a production and resistance. psv1 hybridized with pbr322 containing a cloned fragment of 1.7 x 10(6) daltons from s. coelicolor a3(2) which codes for methylenomycin a resistance. the psv1 plasmid could be transferred to s. liv ... | 1982 | 7142961 |
| identification of streptomyces violaceoruber tü22 genes involved in the biosynthesis of granaticin. | a 50 kb region of dna from streptomyces violaceoruber tü22, containing genes encoding proteins involved in the biosynthesis of granaticin, was isolated. the dna sequence of a 7.3 kb fragment from this region, located approximately 10 kb from the genes that encode the polyketide synthetase responsible for formation of the benzoisochromane quinone skeleton, revealed five open reading frames (orf1-orf5). the deduced amino acid sequence of grae, encoded by orf2, shows 60.8% identity (75.2% similarit ... | 1995 | 7476861 |
| human pancreatic phospholipase a2 stimulates the growth of human pancreatic cancer cell line. | phospholipase a2 (pla2) from human pancreas, designated hpla2-i, functions as a digestive enzyme. interestingly, the present study demonstrated that the mature form of hpla2-i stimulated the growth of a human pancreatic cancer cell line miapaca-2, whereas the pro-form was ineffective. pla2s from laticauda semifasciata fraction i, crotalus adamanteus venom, streptomyces violaceoruber and bee venom, showed no proliferative effect to the growth of miapaca-2. the scatchard plot analysis revealed tha ... | 1995 | 7589440 |
| a sorangium cellulosum (myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen a: cloning, characterization, and homology to polyketide synthase genes from actinomycetes. | a 40-kb region of dna from sorangium cellulosum so ce26, which contains polyketide synthase (pks) genes for synthesis of the antifungal macrolide antibiotic soraphen a, was cloned. these genes were detected by homology to streptomyces violaceoruber genes encoding components of granaticin pks, thus extending this powerful technique for the identification of bacterial pks genes, which has so far been applied only to actinomycetes, to the gram-negative myxobacteria. functional analysis by gene disr ... | 1995 | 7601830 |
| comparative ribosomal protein (l11 and l30) sequence analyses of several streptomyces spp. commonly used in genetic studies. | the taxonomic relationships among nine strains of streptomyces, which have been commonly used for genetic studies, were examined by sequence analysis of their ribosomal l11(= rplk) protein genes. phylogenetic relationships among these organisms derived from similarity sequence analysis of the rplk genes were in good agreement with those derived from the analysis of the deduced l11 protein amino acid sequence itself, indicating complete sequence homology among streptomyces coelicolor a3(2), 'stre ... | 1998 | 9731302 |
| the granaticin biosynthetic gene cluster of streptomyces violaceoruber tü22: sequence analysis and expression in a heterologous host. | the granaticins are members of the benzoisochromanequinone class of aromatic polyketides, the best known member of which is actinorhodin made by streptomyces coelicolor a3(2). genetic analysis of this class of compounds has played a major role in the development of hypotheses about the way in which aromatic polyketide synthases (pkss) control product structure. although the granaticin nascent polyketide is identical to that of actinorhodin, post-pks steps involve different pyran-ring stereochemi ... | 1998 | 9831526 |
| crystallization and preliminary x-ray diffraction studies of streptomyces phospholipase a2 in a calcium-binding form. | phospholipase a2 (pla2) as a calcium-binding form, produced by streptomyces violaceoruber, was crystallized in a form suitable for the diffraction analysis using the vapor diffusion method. crystals were grown in 0.1 m tris-hcl buffer (ph 8.5), 20 mm ca2+ containing 50-60% (v/v) 2-methyl-2,4-pentanediol as a precipitant. they belong to the monoclinic space group p2(1), with the cell dimensions a=38.3 a, b=54.3 a, c=30.6 a, and beta=90.2 degrees. the crystals diffract the x-ray well and the diffr ... | 2000 | 11132631 |
| identification of four genes from the granaticin biosynthetic gene cluster of streptomyces violaceoruber tü22 involved in the biosynthesis of l-rhodinose. | four genes, orf 22 approximately 25, from the granaticin biosynthetic gene cluster of streptomyces violaceoruber tü22 were analyzed for their involvement in the biosynthesis of the two deoxysugar moieties of the granaticins. each gene was individually inactivated on a cosmid carrying the entire gra gene cluster and the mutant cosmids were transformed into s. coelicolor ch999. analysis of the pattern of pigment production by the transformants revealed that each of the four orfs is required for th ... | 2001 | 11269719 |
| functional complementation of pyran ring formation in actinorhodin biosynthesis in streptomyces coelicolor a3(2) by ketoreductase genes for granaticin biosynthesis. | a mutation in actvi-orf1, which controls c-3 reduction in actinorhodin biosynthesis by streptomyces coelicolor, was complemented by gra-orf5 and -orf6 from the granaticin biosynthetic gene cluster of streptomyces violaceoruber tü22. it is hypothesized that, while gra-orf5 alone is a ketoreductase for c-9, gra-orf6 gives the enzyme regiospecificity also for c-3. | 2001 | 11325954 |
| a new mode of stereochemical control revealed by analysis of the biosynthesis of dihydrogranaticin in streptomyces violaceoruber tü22. | a class of streptomyces aromatic polyketide antibiotics, the benzoisochromanequinones, all shows trans stereochemistry at c-3 and c-15 in the pyran ring. the opposite stereochemical control found in actinorhodin (3s, 15r, act) from s. coelicolor a3(2) and dihydrogranaticin (3r, 15s, dhgra) from s. violaceoruber tü22 was studied by functional expression of the potentially relevant ketoreductase genes, actiii, actvi-orf1, gra-orf5, and gra-orf6. a common bicyclic intermediate was postulated to und ... | 2001 | 11707113 |
| the crystal structure of prokaryotic phospholipase a2. | in this study, the x-ray crystal structures of the calcium-free and calcium-bound forms of phospholipase a(2) (pla(2)), produced extracellularly by streptomyces violaceoruber, were determined by using the multiple isomorphous replacement and molecular replacement methods, respectively. the former and latter structures were refined to an r-factor of 18.8% at a 1.4-a resolution and an r-factor of 15.0% at a 1.6-a resolution, respectively. the overall structure of the prokaryotic pla(2) exhibits a ... | 2002 | 11897785 |
| a novel prokaryotic phospholipase a2. characterization, gene cloning, and solution structure. | until now, phospholipase a(2) (pla(2); ec 3.1.14) has been found only from eukaryotic sources. in the present study, we found a secreted pla(2), which is produced by a soil bacterium, streptomyces violaceoruber a-2688, demonstrating that the enzyme is the first phospholipase a(2) identified in prokaryote. after characterization of the novel pla(2), a gene encoding the enzyme was cloned, sequenced, and overexpressed using a streptomyces host-vector system. the amino acid sequence showed that the ... | 2002 | 11897786 |
| oxidation in vivo of the &z.sbnd;sh groups of streptomyces violaceoruber fructokinase. | | 1973 | 11946909 |
| characterization of the streptomyces violaceoruber sank95570 plasmids psv1 and psv2. | we have analyzed the structure of two extrachromosomal elements of the methylenomycin producing actinomycete streptomyces violaceoruber sank95570. the presence of the circular plasmid psv1 which was supposed to contain the genes for methylenomycin biosynthesis could be verified. physical mapping of psv1 revealed a size of 175.35 kb for this plasmid. in addition we generated a restriction map for the 100-kb linear plasmid psv2. cloning and sequencing of the terminal ends of psv2 indicated the pre ... | 2002 | 12127493 |
| atomic resolution structure of prokaryotic phospholipase a2: analysis of internal motion and implication for a catalytic mechanism. | we have found a secreted phospholipase a(2) (pla(2), ec 3.1.1.4) from streptomyces violaceoruber a-2688, which is the first pla(2) identified in prokaryote, and determined its tertiary structure by nmr and x-ray analyses. in this study, we collected the x-ray diffraction data of the bacterial pla(2) at room temperature (297 k) using conventional mok(alpha) radiation and refined the structure at a 1.05 a resolution. the atomic resolution analysis led us to introduce disordered conformations and h ... | 2003 | 12696056 |
| limited regions of homology between linear and circular plasmids encoding methylenomycin biosynthesis in two independently isolated streptomycetes. | psv1 is a plasmid in streptomyces violaceoruber sank95570 that carries the methylenomycin biosynthetic (mmy) gene cluster. an ordered cosmid map and an ecori map have been constructed for psv1, confirming that psv1 is a 163 kb circular plasmid. the mmy gene cluster has been found on three different replicon structures; the circular plasmid psv1, the 356 kb linear plasmid scp1 and, via scp1 integration, the linear chromosome of streptomyces coelicolor a3(2). comparison of psv1 and scp1 sequences ... | 2003 | 12724396 |
| the fine structure of streptomyces violaceoruber (s. coelicolor). iii. the walls of the mycelium and spores. | a study of thin sections of hyphae of streptomyces violaceoruber in the electron microscope showed that the structure of the walls and the mode of formation of cross-walls are similar to those of gram-positive bacteria. a beaded structure was seen in some regions of the wall, and the significance of this observation is discussed in relation to previous studies of the fine structure of bacterial cell walls. elements of the intracytoplasmic membrane system appear to be involved in the process of c ... | 1961 | 13705984 |
| streptomyces coelicolor mueller and streptomyces violaceoruber waksman and curtis, two distinctly different organisms. | | 1959 | 14412998 |
| electron microscope observations on the surface structures of streptomyces violaceoruber. | | 1961 | 14448835 |
| selective isolation of members of the streptomyces violaceoruber clade from soil. | large numbers of filamentous actinomycetes which formed distinctive red coloured colonies were isolated from three out of four composite soil samples using a medium designed to be selective for members of the streptomyces violaceoruber clade, a taxon which includes the model organisms "streptomyces coelicolor" a3(2) and "streptomyces lividans" 66. the isolation medium, dextran-histidine-sodium chloride-mineral salts agar supplemented with antibacterial and antifungal antibiotics, also supported ... | 2005 | 15837389 |
| identification of secondary metabolites from streptomyces violaceoruber tu22 by means of on-flow lc-nmr and lc-dad-ms. | for rapid screening of natural products from actinomycetes, a combination of on-line couplings lc-nmr, lc-dad-ms and hplc-pda, as well as maldi-tof-ms is particularly suitable. simultaneous use of these coupling techniques provides considerable advantages for the rapid identification of natural compounds in mixtures. the results of our present investigation on secondary metabolite products of streptomyces violaceoruber tu 22 showed that more than 50% of the identified metabolites are new compoun ... | 2005 | 16049955 |
| purification and characterization of the second streptomyces phospholipase a2 refolded from an inclusion body. | a secreted phospholipase a(2) (pla(2)) from streptomyces violaceoruber a-2688, previously identified by us, is the first pla(2) identified in prokaryotes. genome sequence data of streptomyces coelicolor a3(2) indicates that the bacterium carries two genes encoding hypothetical pla(2)s, which exhibit 100 and 78% identity, respectively, to the s. violaceoruber pla(2). in this study, we named the former and latter proteins as the first and second pla(2)s, respectively. when the second pla(2) was ex ... | 2006 | 16809051 |
| a type i/type iii polyketide synthase hybrid biosynthetic pathway for the structurally unique ansa compound kendomycin. | kendomycin is a bioactive polyketide that is produced by various streptomyces strains. it displays strong antibiotic activities against a wide range of bacteria and exhibits remarkable cytotoxic effects on the growth of several human cancer cell lines. in this study we cloned the corresponding biosynthetic locus from the producer streptomyces violaceoruber (strain 3844-33c). our analysis shows that a mixed type i/type iii polyketide synthase pathway is responsible for the formation of the fully ... | 2008 | 18972512 |
| streptomyces marokkonensis sp. nov., isolated from rhizosphere soil of argania spinosa l. | the novel actinomycete strain ap1(t) was isolated from rhizosphere soil of the argan tree (argania spinosa l.) in the south of morocco. strain ap1(t) has been reported as a novel producer of the pentaene polyene macrolide isochainin, which strongly inhibits the growth of pathogenic yeasts and phytopathogenic fungi. strain ap1(t) shows a greyish-white aerial mycelium with chains of smooth-surfaced spores of the spiralis type and a cell wall containing ll-diaminopimelic acid. based on chemotaxonom ... | 2009 | 19628602 |
| streptomyces thinghirensis sp. nov., isolated from rhizosphere soil of vitis vinifera. | a novel actinomycete, strain s10(t), was isolated from rhizosphere soil of wild vitis vinifera in thinghir, ouarzazate province, southern morocco. the taxonomic status of this strain was established using a polyphasic approach. strain s10(t) had white-grey aerial mycelium with long, spiral spore chains bearing smooth surfaced spores and produced a yellow diffusible pigment. chemotaxonomic analyses showed that the cell wall of strain s10(t) contained ll-diaminopimelic acid and glycine. phylogenet ... | 2009 | 19643894 |
| granaticins and their biosynthetic gene cluster from streptomyces vietnamensis: evidence of horizontal gene transfer. | streptomyces vietnamensis, a recently designated species isolated from tropical forest soil, was found to be a new granaticin producer. the granaticin biosynthetic gene cluster (gra) and flanking genes from s. vietnamensis were cloned and sequenced by a sequential cloning strategy. all biosynthetic genes were found as expected. the high overall homology of the gra cluster from s. vietnamensis to that of streptomyces violaceoruber t++22 indicated a recent common ancestor of the two clusters. howe ... | 2011 | 21732034 |
| use of an isothermal microcalorimetry assay to characterize microbial oxalotrophic activity. | isothermal microcalorimetry (imc) has been used in the past to monitor metabolic activities in living systems. a few studies have used it on ecological research. in this study, imc was used to monitor oxalotrophic activity, a widespread bacterial metabolism found in the environment, and particularly in soils. six model strains were inoculated in solid angle media with k-oxalate as the sole carbon source. cupriavidus oxalaticus, cupriavidus necator, and streptomyces violaceoruber presented the hi ... | 2011 | 21696406 |
| Extracellular production of phospholipase A(2) from Streptomyces violaceoruber by recombinant Escherichia coli. | Phospholipase A(2) (PLA(2)) from Streptomyces violaceoruber was successfully produced extracellularly in an active form by using a recombinant strain of Escherichia coli. The PLA(2) gene, which was artificially synthesized with optimized codons for E. coli and fused with pelB signal sequence, was expressed in E. coli using pET system. Most of the enzyme activity was detected in the culture supernatant with negligible activity in the cells. The recombinant enzyme was purified to homogeneity from ... | 2012 | 22019762 |
| [Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut]. | We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping. | 2011 | 21866710 |
| removal of odorous compounds from poultry manure by microorganisms on perlite--bentonite carrier. | laboratory-scale experiments were conducted using poultry manure (pm) from a laying hen farm. six strains of bacteria and one strain of yeast, selected on the base of the previous study, were investigated to evaluate their activity in the removal of odorous compounds from poultry manure: pure cultures of bacillus subtilis subsp. spizizenii lock 0272, bacillus megaterium lock 0963, pseudomonas sp. lock 0961, psychrobacter faecalis lock 0965, leuconostoc mesenteroides lock 0964, streptomyces viola ... | 2014 | 24768836 |
| [isolation and identification of termitarium antagonistic actinomycetes byc 01 and its active metabolites]. | we isolated actinomyces from the termitarium and studied its metabolites to find the antimicrobial compounds. | 2014 | 25252456 |
| streptomyces violaceoruber phospholipase a2: expression in pichia pastoris, properties, and application in oil degumming. | the phospholipase a2 (pla2) from streptomyces violaceoruber was successfully expressed in the methylotrophic yeast pichia pastoris gs115 under the control of aox1 promoter for the first time. the maximum activity of the recombinant pla2 (rpla2) reached 34.7 ± 0.2 u/ml, and specific activity was 170 ± 4 u/mg after purification. on the sodium dodecyl sulfate polyacrylamide gel electrophoresis of the culture supernatants, three bands of 21, 18, and 14.3 kda were observed. the peptide mass fingerpri ... | 2015 | 25618786 |
| -genomic data mining of the marine actinobacteria streptomyces sp. h-kf8 unveils insights into multi-stress related genes and metabolic pathways involved in antimicrobial synthesis. | streptomyces sp. h-kf8 is an actinobacterial strain isolated from marine sediments of a chilean patagonian fjord. morphological characterization together with antibacterial activity was assessed in various culture media, revealing a carbon-source dependent activity mainly against gram-positive bacteria (s. aureus and l. monocytogenes). genome mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic gene clusters (bgcs) for secondary metabolites, where among them, ... | 2017 | 28229018 |
| a constant area monolayer method to assess optimal lipid packing for lipolysis tested with several secreted phospholipase a2. | we present an analysis of lipid monolayer hydrolysis at a constant area to assess the optimal lateral surface pressure value (πopt) and thus, the surface packing density of the lipid, at which the activity of a given lipolytic enzyme is maximal. this isochoric method consists of a measurement of the decrease down to zero of the πopt of phospholipid substrate monolayer due to continuous hydrolysis using only one reaction compartment. we performed the comparison of both approaches using several co ... | 2015 | 26051123 |
| poly r decolorization and appl production by streptomyces violaceoruber and streptomyces spiroverticillatus. | two mesophilic streptomycetes (s. violaceoruber and s. spiroverticillatus) were selected to study their poly r-478 decolorization ability and lignocellulose solubilizing activity. both strains were able to degrade poly r-478 dye and ferulic acid during growth on a minimal salts medium. the poly r-478 decolorizing activities of both strains were induced by adding different carbon sources to the culture media. s. violaceoruber could decolorize 63% of poly r-478 after 24 h. both strains could solub ... | 2014 | 25763021 |
| characterization of a replication locus and formation of a higher-order complex between repa protein and two inverted repeats in streptomyces plasmid psv1. | we identified the minimal locus of 163-kb plasmid psv1 of streptomyces violaceoruber for the replication in s. lividans. this locus comprised a repa gene and an upstream 407-bp sequence containing two inverted repeats (ir-iii and ir-iv) within an iteron, an at-rich region and a 300-bp noncoding sequence (ncs). repa protein bound specifically to a 94-bp sequence covering the intact ir-iii and ir-iv to form multimers of dna/protein complexes, but was unable to bind specifically to the ncs and the ... | 2013 | 24152230 |
| identification of novel phospholipase a2 group ix members in metazoans. | sequence homologues of the bacterium streptomyces violaceoruber and sea anemone nematostella vectensis pla2 pfam09056 members were identified in several bacteria, fungi and metazoans illustrating the evolution of this pla2 sub-family. comparison of their molecular structures revealed that bacteria and fungi members are part of the gxiv of pla2s while metazoan representatives are similar with gix pla2 of the marine snail conus magus. members of gxiv and gix pla2s show modest overall sequence simi ... | 2013 | 23603114 |
| statistical optimization of alkaline xylanase production from streptomyces violaceoruber under submerged fermentation using response surface methodology. | response surface methodology employing central composite design (ccd) was used to optimize fermentation medium for the production of cellulase-free, alkaline xylanase from streptomyces violaceoruber under submerged fermentation. the design was employed by selecting wheat bran, peptone, beef extract, incubation time and agitation as model factors. a second-order quadratic model and response surface method showed that the optimum conditions for xylanase production (wheat bran 3.5 % (w/v), peptone ... | 2007 | 23100657 |