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a phylogenetic analysis of the family dermatophilaceae.the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ...19836195302
morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces.in an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16s rdna sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. all except one strain, which was found to be a bacillus, were members of the order actinomycetales. the majority of the strains either were closely related to geodermatophilus obscurus, which was al ...19968661940
flagellar proteins of motile spores of actinomycetes.flagella of some of the actinoplanete genera were purified and the molecular sizes of their flagellin subunits compared by sds-page analysis to flagellins of cells of other bacteria. several species of actinoplanes have a major flagellar protein of subunit sizes of 42-43 kda and a lesser amount of a second protein, possibly a minor flagellin subunit, of 60 kda. the flagellar protein sizes of other actinoplanetes ranged from 32-43 kda (major) and 48-58 kda (minor). antibodies formed against the 4 ...19968987497
modestobacter multiseptatus gen. nov., sp. nov., a budding actinomycete from soils of the asgard range (transantarctic mountains).oligotrophic pygv medium, inoculated with soils from linnaeus terrace (1600 m, antarctica), yielded four aerobic actinomycetes with short rods, multiple and irregular septa and often motile buds. cells were 1.0-2.8 x 1.0-3.0 microm and colonies were beige to pink. the isolates were nearly identical in physiological and biochemical tests. three strains grew from 0 degrees c to 25-28 degrees c, but one was psychrophilic with a maximum growth temperature of 20 degrees c. carbon sources utilized wer ...200010826821
design and application of two oligonucleotide probes for the identification of geodermatophilaceae strains using fluorescence in situ hybridization (fish).bacteria of the family of geodermatophilaceae are actively involved in the decay processes [urzì, c. and realini, m. (1998) int biodeterior biodegrad 42: 45-54; urzì, c., salamone, p., schumann, p., and stackebrandt, e. (2000) int j syst evol microbiol 50: 529-536] of stone monuments. characterization of isolates includes phenotypic, chemotaxonomic and genetic analysis often requiring long-term procedures. the use of specific probes for members of geodermatophilaceae family could be useful for t ...200415186346
quadrisphaera granulorum gen. nov., sp. nov., a gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules.a gram-positive bacterium, designated strain ag019(t), was isolated by micromanipulation from aerobic granules obtained from a laboratory-scale sequencing batch reactor. this isolate grew axenically as cocci clustered predominantly in tetrads, and was morphologically similar to the dominant organisms observed in the biomass. the morphology also resembled that of the tetrad-forming organisms commonly seen in activated sludge samples. strain ag019(t) was found to be an oxidase-negative, catalase-p ...200516166665
geodermatophilus, a new genus of the dermatophilaceae (actinomycetales).organisms having a complex life cycle similar to the animal pathogen dermatophilus congolensis have been isolated from soils of the united states. the soil isolates grow readily on agar media producing a black, friable, or sooty colony; in broth, the isolates produce a thick sediment with a clear supernatant fluid and occasionally a black surface pellicle. the generic taxon geodermatophilus is proposed for these soil isolates. geodermatophilus differs morphologically from dermatophilus in thallu ...19685726312
control of morphogenesis in geodermatophilus: ultrastructural studies.geodermatophilus grows in two major forms, a nonmotile irregularly shaped aggregate of coccoid cells (c-form) and a motile budding rod (r-form). morphogenesis can be controlled by an unidentified factor in tryptose which is required for maintenance of the organism in the c-form and for differentiation of the r-form to the c-form. morphogenetic events occur synchronously in the described system. ultrastructural studies show that the major difference between c- and r-forms is in cells envelope str ...19705473909
pathogenicity of dermatophilus and geodermatophilus.cutaneous infection in laboratory animals could not be induced with any of several strains of geodermatophilus. a model for consistent production of streptotrichosis in rabbits, with cultures of dermatophilus congolensis, is presented.19715154874
induction of morphogenesis in geodermatophilus by inorganic cations and by organic nitrogenous cations.morphogenesis of geodermatophilus strain 22-68 involves two stages, a motile rod (r form) and an irregularly shaped cluster of coccoid cells (c form). a variety of mono- and divalent cations have been found to induce r-form to c-form morphogenesis and to maintain the organism in the c form. concentration optima for all cations exceeded 100 mm. results indicated that uptake of cations was accompanied by extrusion of intracellular protons, causing an increase in intracellular ph. a variety of orga ...19744587602
complete genome sequence of geodermatophilus obscurus type strain (g-20).geodermatophilus obscurus luedemann 1968 is the type species of the genus, which is the type genus of the family geodermatophilaceae. g. obscurus is of interest as it has frequently been isolated from stressful environments such as rock varnish in deserts, and as it exhibits interesting phenotypes such as lytic capability of yeast cell walls, uv-c resistance, strong production of extracellular functional amyloid (fuba) and manganese oxidation. this is the first completed genome sequence of the f ...201021304698
geodermatophilus ruber sp. nov., isolated from rhizosphere soil of a medicinal plant.a novel actinobacterial strain, designated cpcc 201356(t), was isolated from a rhizosphere soil sample of the medicinal plant astragalus membranaceus and subjected to a polyphasic taxonomic analysis. good growth occurred at 20-32 °c, at ph 7.0-7.5 and with 0-1 % (w/v) nacl. colonies on r2a and isp 2 agar were light red to red, round and lacked aerial mycelium; cells adhered to the agar. the peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. the predominant menaquino ...201120190022
esterase as an enzymatic signature of geodermatophilaceae adaptability to sahara desert stones and monuments.to assess esterase profiling of members of geodermatophilaceae isolated from desert stones and monuments in tunisia and egypt.201019878364
effect of propargyl bromide and 1,3-dichloropropene on microbial communities in an organically amended soil.abstract in this study we investigated the response of microbial communities in unamended and manure-amended soil treated with the fumigants propargyl bromide (pbr) and 1,3-dichloropropene (1,3-d). the soil fumigants were applied at a rate of 10, 100, and 500 mg kg(-1). after treatment of the soils, the metabolic activity was assessed by monitoring the dehydrogenase activity (dha). pbr and 1,3-d initially inhibited the dha when applied at 500 mg kg(-1); however, after 8 weeks, recovery of the dh ...200319719698
widespread abundance of functional bacterial amyloid in mycolata and other gram-positive bacteria.until recently, extracellular functional bacterial amyloid (fuba) has been detected and characterized in only a few bacterial species, including escherichia coli, salmonella, and the gram-positive organism streptomyces coelicolor. here we probed gram-positive bacteria with conformationally specific antibodies and revealed the existence of fuba in 12 of 14 examined mycolata species, as well as six other distantly related species examined belonging to the phyla actinobacteria and firmicutes. most ...200919395568
phylogenetic analysis of actinobacterial populations associated with antarctic dry valley mineral soils.despite the apparent severity of the environmental conditions in the mcmurdo dry valleys, eastern antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest phylotypic groups. previous metagenomic studies have shown that the majority of antarctic actinobacterial populations are classified as 'uncultured'. in this study, we assessed the diversity of actinobacteria i ...200919278445
life on the rocks.biofilms are interface micro-habitats formed by microbes that differ markedly from those of the ambient environment. the term 'subaerial biofilm' (sab) was coined for microbial communities that develop on solid mineral surfaces exposed to the atmosphere. subaerial biofilms are ubiquitous, self-sufficient, miniature microbial ecosystems that are found on buildings, bare rocks in deserts, mountains, and at all latitudes where direct contact with the atmosphere and solar radiation occurs. subaerial ...200717564597
blastococcus jejuensis sp. nov., an actinomycete from beach sediment, and emended description of the genus blastococcus ahrens and moll 1970.a novel actinomycete, strain kst3-10(t), was isolated from sand sediment of a beach in jeju, korea, and was subjected to polyphasic taxonomic characterization. the organism produced circular, smooth, translucent, apricot-coloured colonies comprising coccoid- or rod-shaped cells. phylogenetic analyses based on 16s rrna gene sequences showed that the organism belonged to the family geodermatophilaceae and consistently formed a distinct sub-branch outside the radiation of the genus blastococcus. th ...200617012568
geodermatophilaceae fam. nov., a formal description.the family name 'geodermatophilaceae' was first published by normand et al. (1996) to contain the genera geodermatophilus and blastococcus, but a formal description and the designation of the type genus were not included, thus making the name invalid. since then, the genus modestobacter has been described as a member of the family 'geodermatophilaceae' by mevs et al. (2000). we hereby formally describe the family 'geodermatophilaceae' which includes the recently described genus modestobacter. th ...200617012547
extensive diversity of ionizing-radiation-resistant bacteria recovered from sonoran desert soil and description of nine new species of the genus deinococcus obtained from a single soil sample.the ionizing-radiation-resistant fractions of two soil bacterial communities were investigated by exposing an arid soil from the sonoran desert and a nonarid soil from a louisiana forest to various doses of ionizing radiation using a (60)co source. the numbers of surviving bacteria decreased as the dose of gamma radiation to which the soils were exposed increased. bacterial isolates surviving doses of 30 kgy were recovered from the sonoran desert soil, while no isolates were recovered from the n ...200516151108
biodiversity of geodermatophilaceae isolated from altered stones and monuments in the mediterranean basin.an investigation was made into the occurrence and biodiversity of geodermatophilaceae on 78 samples of altered stone surfaces from 24 monuments and natural stones in the mediterranean basin; it was found that the total microbial counts ranged between 0 and 10(7) cfu g(-1) dry weight. members of the geodermatophilaceae family were isolated from 22 of the 78 samples examined, with the incidence of geodermatophilaceae colonies in the cultivable population ranging from 1% to 100%. the highest percen ...200111553237
[study of the lytic activity of actinomycetes isolated from various soils in georgia].the lytic activities of 310 cultures from the collection of actinomycetes of the institute of biochemistry and biotechnology, national academy of sciences of georgia, were studied; 18% of these strains appeared capable of lysing the yeast cell wall. the active producer of the enzyme was selected. this culture was isolated from chestnut soil in gardabani raion (central georgia). its cultural-morphological, biochemical, and antagonistic properties allowed the culture to be ascribed to the species ...200111443896
application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter.the present paper describes a simple enrichment technique which enables rapid and selective isolation of diverse zoosporic actinomycete genera directly from soil and plant litter. this technique, designated the rehydration and centrifugation (rc) method, consists of immersing the air-dried source material in 10 mm phosphate buffer containing 10% soil extract, letting the preparation stand at 30 degrees c for 90 min, followed by centrifugation of the fluid at 1,500 x g for 20 min. portions of the ...200011204769
a reca gene phylogenetic analysis confirms the close proximity of frankia to acidothermus.the closer proximity of frankia and acidothermus cellulolyticus relative to the morphologically close geodermatophilus found previously was confirmed by resequencing the rrs gene of acidothermus cellulolyticus and the housekeeping gene, reca. the diagnostic sugar 2-o-methyl-d-mannose was detected only in frankia, while hopanoid lipids were present at high levels in both acidothermus and frankia.200010758889
molecular phylogeny of the genus frankia and related genera and emendation of the family frankiaceae.the members of the actinomycete genus frankia are nitrogen-fixing symbionts of may species of woody dicotyledonous plants belonging to eight families. several strains isolated from diverse actinorhizal plants growing in different geographical areas were used in this study. the phylogenetic relationships of these organisms and uncharacterized microsymbionts that are recalcitrant to isolation in pure culture were determined by comparing complete 16s ribosomal dna sequences. the resulting phylogene ...19968573482
numerical classification of sporoactinomycetes containing meso-diaminopimelic acid in the cell wall.one hundred and thirty actinomycetes representing 19 genera and 50 species were compared in a numerical phenetic survey using 108 unit characters. data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering was achieved using both the single and unweighted pair group average algorithms. cluster composition was barely affected by the statistics used or by test error, estimated at 2.1%. over 80% of the strains were assigned to 2 clusters containing ...19827077300
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