| isolation and characterization of actinopolyspora halophila, gen. et sp. nov., an extremely halophilic actinomycete. | an actinomycete, isolated as a contaminant of a culture medium containing 25% nacl, has been classified as actinopolyspora halophila gen. et sp. nov. in the family nocardiaceae. the morphology and biochemical characteristics of this organism distinguish it from other members of the family nocardiaceae and other genera possessing a type iv cell wall. it requires high nacl concentrations for growth and can grow in saturated nacl. the lowest concentration permitting growth in liquid medium is 12%, ... | 1975 | 1201505 |
| amino acid sequence analysis of ribosomal protein at-l30 from members of the family pseudonocardiaceae. | the phylogenetic relationships of the genera belonging to the family pseudonocardiaceae were examined by a novel approach, amino acid sequencing of ribosomal at-l30 proteins. the results of partial amino acid sequencing of at-l30 preparations revealed that the members of the family pseudonocardiaceae are divided into four clusters; the first cluster contains the genus actinopolyspora, the second cluster contains the genus saccharopolyspora, the third cluster contains the genus amycolatopsis, and ... | 1995 | 7857791 |
| three new species of the genus actinobispora of the family pseudonocardiaceae, actinobispora alaniniphila sp. nov., actinobispora aurantiaca sp. nov. and actinobispora xinjiangensis sp. nov. | the genus actinobispora jiang et al. is characterized by the formation of longitudinally paired spores on both the vegetative and the aerial mycelium, absence of mycolic acid, cell wall chemotype iv (containing meso-diaminopimelic acid as the diamino acid, galactose and arabinose), pvi and mk-9(h2). comparative studies of morphology, chemical classification and phylogenetic analysis based on the 16s rrna sequences among the four type strains of the genus actinobispora and type strains of related ... | 1999 | 10319514 |
| multiplex specific pcr for identification of the genera actinopolyspora and streptomonospora, two groups of strictly halophilic filamentous actinomycetes. | the genera actinopolyspora and streptomonospora are two groups of extremely halophilic filamentous actinomycetes. members of these two genera are isolated frequently, probably due to the high occurrence of these actinomycetes in the hypersaline soil environment. although members of these genera can be identified by micromorphological criteria, the extensive chemotaxonomic characterization of each new isolates is a time-consuming task which cannot always be undertaken when handling large numbers ... | 2007 | 17308865 |
| erythronolides h and i, new erythromycin congeners from a new halophilic actinomycete actinopolyspora sp. yim90600. | erythronolides h and i, novel congeners of the clinically important antibacterial drug erythromycin a, have been isolated from the new halophilic actinomycete actinopolyspora sp. yim90600. in addition to producing the new erythromycin congeners, a. sp. yim90600 produces erythromycin c in a high titer. the presence of the c-14 hydroxyl moiety and the c-6/c-18-epoxide in erythronolide h and the spiroketal moiety of erythronolide i sheds new insights into structural diversity of erythromycin analog ... | 2009 | 19228040 |
| an update of the structure and 16s rrna gene sequence-based definition of higher ranks of the class actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. | the higher ranks of the class actinobacteria were proposed and described in 1997. at each rank, the taxa were delineated from each other solely on the basis of 16s rrna gene sequence phylogenetic clustering and taxon-specific 16s rrna signature nucleotides. in the past 10 years, many novel members have been assigned to this class while, at the same time, some members have been reclassified. the new 16s rrna gene sequence information and the changes in phylogenetic positions of some taxa influenc ... | 2009 | 19244447 |
| actinopolyspora xinjiangensis sp. nov., a novel exteremely halophilic actinomycete isolated from a salt lake in xinjiang, china. | a novel halophilic, filamentous actinobacterium, designated trm 40136(t), was isolated from a hypersaline habitat in xinjiang province, north-west china. the strain is aerobic, gram-positive, halophilic, and the optimum nacl concentration for growth is 10-15% (w/v). the whole-cell sugar pattern consists of xylose, glucose and arabinose. the predominant menaquinone is mk-6 (51.2%) and the major fatty acids are anteiso-c15:0 (35.2%), anteiso-c17:0 (15.9%) and iso-c15:0 (13.7%). the phospholipid pa ... | 2010 | 20524152 |
| actinopolyspora alba sp. nov. and actinopolyspora erythraea sp. nov., isolated from a salt field in china, and reclassification of actinopolyspora iraqiensis as 4.1193t (ruan et al. 1994) as a later heterotypic synonym of saccharomonospora halophila. | halophilic actinomycetal strains yim 90479, yim 90480t and yim 90600t were isolated from a salt field in xinjiang province, north-west of china and subjected to polyphasic taxonomy. all three strains were moderately halophilic, no growth in absence of nacl, and were able to grow on agar plate at nacl concentrations of up to 20 or 25 % (w/v). good growth occurred at 37 degrees c and 15% (w/v) nacl. all their cell walls were type iv. phospholipid pattern was type piii. mk-9 (h4), mk-10 (h4) or mk- ... | 2010 | 20729305 |
| halophilic actinomycetes in saharan soils of algeria: isolation, taxonomy and antagonistic properties. | the diversity of a population of 52 halophilic actinomycetes was evaluated by a polyphasic approach, which showed the presence of actinopolyspora, nocardiopsis, saccharomonospora, streptomonospora and saccharopolyspora genera. one strain was considered to be a new member of the last genus and several other strains seem to be new species. furthermore, 50% of strains were active against a broad range of indicators and contained genes encoding polyketide synthetases and nonribosomal peptide synthet ... | 2011 | 21764956 |
| bioweathering and biotransformation of granitic rock minerals by actinomycetes. | actinomycetes inhabiting granitic rocks at st. katherine, egypt were investigated for their bioweathering potential. actinomycete counts ranged between 174 and 360 colony forming units per gram. counts were positively correlated to rock porosity (r = 0.65) and negatively correlated to rock salinity (r = -0.56). sixty-six actinomycete isolates originating from rocks could be assigned into eight genera, with a high frequency of nocardioides and streptomyces. organic acids were produced by 97% of t ... | 2009 | 19590809 |
| actinopolyspora halophila has two separate pathways for betaine synthesis. | the extremely halophilic actinomycete actinopolyspora halophila is a rare example of a heterotrophic eubacterium producing betaine from simple carbon sources. a. halophila synthesized remarkably high intracellular concentrations of betaine. the highest betaine concentration, determined at 24% (w/v) nacl, was 33% of the cellular dry weight. trehalose was synthesized as a compatible solute, accounting for up to 9.7% of the cellular dry weight. the betaine concentration was shown to increase with i ... | 2001 | 11685374 |
| extreme halophiles synthesize betaine from glycine by methylation. | glycine betaine is a compatible solute, which is able to restore and maintain osmotic balance of living cells. it is synthesized and accumulated in response to abiotic stress. betaine acts also as a methyl group donor and has a number of important applications including its use as a feed additive. the known biosynthetic pathways of betaine are universal and very well characterized. a number of enzymes catalyzing the two-step oxidation of choline to betaine have been isolated. in this work we hav ... | 2000 | 10896953 |
| actinopolysporins a-c and tubercidin as a pdcd4 stabilizer from the halophilic actinomycete actinopolyspora erythraea yim 90600. | our current natural product program utilizes new actinomycetes originating from unexplored and underexplored ecological niches, employing cytotoxicity against a selected panel of cancer cell lines as the preliminary screen to identify hit strains for natural product dereplication, followed by mechanism-based assays of the purified natural products to discover potential anticancer drug leads. three new linear polyketides, actinopolysporins a (1), b (2), and c (3), along with the known antineoplas ... | 2011 | 21870828 |