| effect of three nematicides on the growth of some phytopathogenic bacteria and fungi. | the effect of three nematicides, aldicarb, fensulfothion, and phenamiphos at four concentrations (1, 5, 25, and 125 ppm) was tested on the growth of five bacteria, agrobacterium tumefaciens, corynebacterium fascians, erwinia carotovora, pseudomonas solanacearum, and streptomyces scabies and four fungi, fusarium oxysporum f. sp. vasinfectum, fusarium solani, rhizoctonia solani, and sclerotium bataticola. of the bacteria, p. solanacearum was most affected by the chemicals at all concentrations, wh ... | 1977 | 143857 |
| isolation and identification of ribosyl-cis-zeatin from transfer rna of corynebacterium fascians. | | 1977 | 603646 |
| zeatin ribonucleosides in the transfer ribonucleic acid of rhizobium leguminosarum, agrobacterium tumefaciens, corynebacterium fascians, and erwinia amylovora. | until recently, the presence in transfer ribonucleic acid (trna) of the hydroxylated cytokinin ribosylzeatin [n6-(4-hydroxy-3-methylbut-2-enyl)adenosine]was thought to be unique to higher plants. this extension of work from several laboratories indicates the presence of 2-methylthioribosylzeatin in the trna of the plant-associated bacteria rhizobium leguminosarum, agrobacterium tumefaciens, and corynebacterium fascians, but not in that of erwinia amylovora. this cytokinin has the cis configurati ... | 1977 | 893341 |
| mechanism of d-alanine production by corynebacterium fascians. | the amounts of extracellular d-alanine accumulated by corynebacterium fascians atcc 21950 in a medium containing glycerol as the sole carbon source is increased to almost 12 mg/ml by adding pyruvate to the medium. cell-free extracts of c. fascians were shown to possess both l-alanine dehydrogenase and alanine racemase activities. these results indicated that a mechanism exists that allows this microorganism to synthesize d-alanine from pyruvate. a study comparing the optical purity of the intrac ... | 1976 | 970932 |
| the plasmid-encoded chloramphenicol-resistance protein of rhodococcus fascians is homologous to the transmembrane tetracycline efflux proteins. | the nucleotide sequence of the chloramphenicol-resistance gene (cmr) of rhodococcus fascians ncppb 1675 (located on the conjugative plasmid prf2) allowed the identification of two possible open reading frames (orfs), of which orf1 was consistent with the mutational analysis. biochemical analysis of cmr revealed that it does not encode an antibiotic-modifying enzyme. the amino acid sequence of orf1 predicted a hydrophobic protein, with 12 putative membrane-spanning domains, homologous to proteins ... | 1992 | 1406276 |
| fasciation induction by the phytopathogen rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. | rhodococcus fascians is a nocardiform bacteria that induces leafy galls (fasciation) on dicotyledonous and several monocotyledonous plants. the wild-type strain d188 contained a conjugative, 200 kb linear extrachromosomal element, pfid188. linear plasmid-cured strains were avirulent and reintroduction of this linear element restored virulence. pulsed field electrophoresis indicated that the chromosome might also be a linear molecule of 4 megabases. three loci involved in phytopathogenicity have ... | 1992 | 1547783 |
| illegitimate integration of non-replicative vectors in the genome of rhodococcus fascians upon electrotransformation as an insertional mutagenesis system. | electrotransformation of rhodococcus fascians by non-replicating plasmids containing a suitable resistance marker resulted in stable transformants by integration of these constructs at various sites in the genome, thereby generating different mutations. tagged genes could be isolated in escherichia coli owing to the presence of a coie1 replicon and an ampicillin resistance gene in the inserted sequences. southern analysis and nucleotide sequencing revealed that recombination can occur at defined ... | 1991 | 1662759 |
| rhodococcus luteus and rhodococcus erythropolis chronic endophthalmitis after lens implantation. | | 1991 | 1951602 |
| nucleotide sequence of 5s ribosomal rna of rhodococcus fascians. | | 1989 | 2762130 |
| carotenoid pigments of genus rhodococcus. | a study of carotenoid pigments of the genus rhodococcus was carried out. according to carotenes contained, rhodococcus species were divided into three groups: the first group of rhodococcus luteus, r. coprophilus, r. lentifragmentus, and r. maris, which formed beta-carotene; the second group of r. equi, r. rubropertinctus, r. aichiensis, r. sputi, r. chubuensis, r. obuensis, r. bronchialis, r. roseus, r. rhodochrous, r. rhodnii, and r. terrae, which formed gamma-carotene-like substance; and the ... | 1989 | 2770561 |
| conjugative transfer of cadmium resistance plasmids in rhodococcus fascians strains. | the presence of a 138-kilobase plasmid (pd188) correlated with increased resistance to cadmium in rhodococcus fascians d188. this plasmid could be transferred by a conjugation-like system in matings between r. fascians strains. transconjugants expressed the cadmium resistance and could be used as donors in subsequent matings. four other r. fascians strains (ncppb 1488, ncppb 1675, ncppb 2551, and atcc 12974) could also be used as donors for cadmium resistance in matings. strain ncppb 1675 showed ... | 1988 | 3162908 |
| isolation of cytokinins from corynebacterium fascians. | | 1966 | 4226130 |
| cytokinins: identification of compounds isolated from corynebacterium fascians. | | 1966 | 4226131 |
| production of d-alanine by corynebacterium fascians. | a strain identified as corynebacterium fascians was found to accumulate extracellular d-alanine from glycerol. cultural conditions for the accumulation of d-alanine were investigated and, as a result, a yield of 7 g of d-alanine per liter was obtained after a 96-h incubation in a medium containing 5% glycerol, 4% (nh(4))(2)hpo(4), and 0.3% corn steep liquor. optical purity of d-alanine was dependent upon the concentration of corn steep liquor. at the optimal condition, almost optically pure d-al ... | 1973 | 4699220 |
| studies on induced mutants of corynebacterium fascians and on their pathogenicity in comparison with that of 'natural' strains. | | 1965 | 5826859 |
| avirulent isolates of corynebacterium fascians that are unable to utilize agmatine and proline. | growth of a highly virulent strain of the phytopathogen corynebacterium fascians on rich media at 37 degrees c resulted in a loss of virulence in a majority of the population within 10 generations. strains retained virulence during cultivation at 30 degrees c on a minimal medium with ammonia as a nitrogen source. populations of avirulent strains on the surfaces of pea seedlings decreased, whereas the number of cells of the virulent strain increased 1,000-fold during a 3-week period. all avirulen ... | 1986 | 3729405 |
| characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (pcb)-degrading strains of comamonas testosteroni isolated from pcb-contaminated soil. | in this study, we isolated and characterized biphenyl (bp) and polychlorinated biphenyl (pcb) degrading bacterial strains found in pcb-contaminated soil from an auto manufacturing plant located in syracuse, new york. twenty-one bp and pcb-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. of the 21 bacteria, 13 were identified as comamonas testosteroni, constituting about 60% of the bacterial population examined. other pcb d ... | 1995 | 7641143 |
| sequence and molecular characterization of a dna region encoding the dibenzothiophene desulfurization operon of rhodococcus sp. strain igts8. | dibenzothiophene (dbt), a model compound for sulfur-containing organic molecules found in fossil fuels, can be desulfurized to 2-hydroxybiphenyl (2-hbp) by rhodococcus sp. strain igts8. complementation of a desulfurization (dsz) mutant provided the genes from rhodococcus sp. strain igts8 responsible for desulfurization. a 6.7-kb taqi fragment cloned in escherichia coli-rhodococcus shuttle vector prr-6 was found to both complement this mutation and confer desulfurization to rhodococcus fascians, ... | 1995 | 7574582 |
| breakdown of indol-3-ylacetic acid by corynebacterium fascians (tilford) dowson. | | 1971 | 5145879 |
| isolation of insertion elements from gram-positive brevibacterium, corynebacterium and rhodococcus strains using the bacillus subtilis sacb gene as a positive selection marker. | the sacb gene of bacillus subtilis was successfully applied in various arthrobacter, brevibacterium, corynebacterium and rhodococcus strains for the isolation of transposable elements. three different insertion sequence (is) elements entrapped in sacb were isolated. the is elements is-bl and is-cg isolated from brevibacterium lactofermentum and corynebacterium glutamicum, respectively, were found to be similar in size (1.45 kb) and generated target duplications of 8 bp. their inverted repeats sh ... | 1995 | 7896070 |
| cloning and sequence determination of the gene coding for the elongation factor tu of mycobacterium leprae. | elongation factor tu (ef-tu) plays an important role in protein biosynthesis and is susceptible to antibiotics in prokaryotes like escherichia coli. in order to understand the primary structure of ef-tu in the intracellular pathogenic bacterium mycobacterium leprae, the gene (tuf gene) coding for this protein was cloned and sequenced. the gene contains a coding region of 1,188 bp with gug as start codon. the deduced amino acid sequence has 396 amino acids with a molecular weight of 43.6 kda. put ... | 1994 | 8089081 |
| cloning and sequence analysis of the gene encoding isocitrate lyase from rhodococcus fascians. | an isocitrate lyase (icl)-encoding gene (icl) from the gram+ plant pathogen rhodococcus fascians was identified serendipitously as part of a scrambled fragment after shotgun cloning in the promoter probe vector, pdp1. the icl protein is 429 amino acids long (47.11 kda) and has a predicted pi of 4.84; it is 54% similar to the escherichia coli icl and 24-27% to eukaryotic homologues. comparison of the prokaryotic and eukaryotic icl confirms the earlier proposal of matsuoka and mcfadden [j. bacteri ... | 1994 | 8045408 |
| the fas operon of rhodococcus fascians encodes new genes required for efficient fasciation of host plants. | three virulence loci (fas, att, and hyp) of rhodococcus fascians d188 have been identified on a 200-kb conjugative linear plasmid (pfid188). the fas locus was delimited to a 6.5-kb dna fragment by insertion mutagenesis, single homologous disruptive recombination, and in trans complementation of different avirulent insertion mutants. the locus is arranged as a large operon containing six open reading frames whose expression is specifically induced during the interaction with host plants. one pred ... | 1994 | 8169198 |
| improved electroporation of rhodococcus equi. | the condition of an electroporation method was re-evaluated for the introduction of foreign plasmid dna into rhodococcus equi. the method is based on an electroporation of the bacteria made competent by culturing in a broth containing glycine and by heat shock at 50 degrees c. transformation of r. equi could be achieved with a chloramphenicol-resistant shuttle vector originating from rhodococcus fascians at an efficiency of about 10(4) transformants/microgram dna. the bacteria were also shown to ... | 1998 | 9524960 |
| characterization of the rrnb operon of the plant pathogen rhodococcus fascians and targeted integrations of exogenous genes at rrn loci. | a 6.0-kb sali dna fragment containing an entire rrna operon (rrnb) was cloned from a cosmid gene bank of the phytopathogenic strain rhodococcus fascians d188. the nucleotide sequence of the 6-kb fragment was determined and had the organization 16s rrna-spacer-23s rrna-spacer-5s rrna without trna-encoding genes in the spacer regions. the 5' and 3' ends of the mature 16s, 23s, and 5s rrnas were determined by alignment with the rrn operons of bacillus subtilis and other gram-positive bacteria. four ... | 1998 | 9546162 |
| purification and characterization of limonoate dehydrogenase from rhodococcus fascians. | limonoate dehydrogenase from rhodococcus fascians has been purified to electrophoretic homogeneity by a procedure that consists of ion-exchange, hydrophobic, and affinity chromatography. the native enzyme has a molecular mass of around 128,000 da and appears to be composed of four similar subunits (30,000 da each). the isoelectric point is 4.9 as determined by isoelectric focusing. the homogeneous enzyme was used to determine the nh2-terminal amino acid sequence. the enzyme was purified from cel ... | 1997 | 9292989 |
| pulsed-field gel electrophoresis analysis of the genome of rhodococcus fascians: genome size and linear and circular replicon composition in virulent and avirulent strains. | total dna of virulent and avirulent strains of rhodococcus fascians was resolved by pulsed-field gel electrophoresis (pfge) into a discrete number of fragments by digestion with the endonucleases asei and drai. restriction endonucleases paci, pmei, and swai yielded no fragments upon digestion of r. fascians genome, and all the other tested endonucleases recognizing 6 bp released too many fragments. the genome size was 5.6 megabases for the type strain r. fascians dsm 20669, and 5.8 megabases for ... | 1998 | 9541568 |
| identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. | coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ... | 1997 | 9039559 |
| isolation of a gene encoding a glycosylated cytokinin oxidase from maize. | the major cytokinin oxidase in immature maize kernels was purified to homogeneity. selected tryptic peptides were used to design degenerate oligonucleotide primers for pcr isolation of a fragment of the oxidase gene. hybridization of the pcr fragment to a maize genomic library allowed isolation of a full-length cytokinin oxidase gene (ckx1). the gene encodes a protein of approximately 57 kda that possesses a signal peptide, eight consensus n-glycosylation sequences and a consensus fad binding se ... | 1999 | 10049708 |
| novel rhodococci and other mycolate actinomycetes from the deep sea. | a large number of mycolate actinomycetes have been recovered from deep-sea sediments in the nw pacific ocean using selective isolation methods. the isolates were putatively assigned to the genus rhodococcus on the basis of colony characteristics and mycolic acid profiles. the diversity among these isolates and their relationship to type strains of rhodococcus and other mycolate taxa were assessed by curie point pyrolysis mass spectrometry (pyms). three major (a, c, d) and two minor (b, e) groups ... | 1998 | 10068786 |
| cloning of an orf with homology to mycobacterium echa1, encoding the enoyl-coa hydratase, in rhodococcus fascians. | an open reading frame encoding a polypeptide of significant homology (55.7% identity) with the enoyl-coa hydratase encoded by the gene echa1 from mycobacterium tuberculosis has been found in the genome of the plant-pathogen bacteria rhodococcus fascians strain nrrl-b-15096. sequence alignments showed that it possesses several conserved blocks common to e. coli, m. tuberculosis and human mitochondria. one of such blocks includes a glutamate residue located at position 149, corresponding to the gl ... | 1999 | 10727085 |
| characterization of antarctic hydrocarbon-degrading bacteria capable of producing bioemulsifiers. | during screening for biosurfactant-producing, n-alkane-degrading marine bacteria, two heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from terra nova bay (ross sea, antarctica) by using aliphatic and artomatic hydrocarbons as the principal carbon source. these gram-positive, aerobic, cocci-shaped bacteria use a various number of organic compounds, including aliphatic hydrocarbons, volatile fatty acids, and biphenyl. during cultivation on n-alkanes as sole sou ... | 1999 | 10423744 |
| the role of apoptosis in normal ontogenesis and solid human neoplasms. | mammalian cells are capable of committing "active suicide" or apoptosis in response to specialized pathological mechanisms employing a phylogenetically developed intrinsic program of death, triggered by signal transduction through specific receptors. changes in cellular structure such as: 1) condensation of the nuclear (chromatin) and cytoplasmic structures (especially the mitochondria); 2) blebbing of the cell membrane; 3) characteristic swelling of the endoplasmic reticulum; and 4) fragmentati ... | 2000 | 11204498 |
| characterization of activity and expression of isocitrate lyase in mycobacterium avium and mycobacterium tuberculosis. | analysis by two-dimensional gel electrophoresis revealed that mycobacterium avium expresses several proteins unique to an intracellular infection. one abundant protein with an apparent molecular mass of 50 kda was isolated, and the n-terminal sequence was determined. it matches a sequence in the m. tuberculosis database (sanger) with similarity to the enzyme isocitrate lyase of both corynebacterium glutamicum and rhodococcus fascians. only marginal similarity was observed between this open readi ... | 1999 | 10572116 |
| the rhodococcus fascians-plant interaction: morphological traits and biotechnological applications. | rhodococcus fascians is a gram-positive bacterium that infects dicotyledonous and monocotyledonous plants, leading to an alteration in the normal growth process of the host. the disease results from the modulation of the plant hormone balances, and cytokinins are thought to play an important role in the induction of symptoms. generally, on the aerial parts of the plants, existing meristems were found to be most sensitive to the action of r. fascians, but, depending on the infection procedure, di ... | 2000 | 10664130 |
| leafy gall formation is controlled by fasr, an arac-type regulatory gene in rhodococcus fascians. | rhodococcus fascians can interact with many plant species and induce the formation of either leafy galls or fasciations. to provoke symptoms, r. fascians strain d188 requires pathogenicity genes that are located on a linear plasmid, pfid188. the fas genes are essential for virulence and constitute an operon that encodes, among other functions, a cytokinin synthase gene. expression of the fas genes is induced by extracts of infected plant tissue only. we have isolated an arac-type regulatory gene ... | 2000 | 11004184 |
| de novo cortical cell division triggered by the phytopathogen rhodococcus fascians in tobacco. | plant growth, development, and morphology can be affected by several environmental stimuli and by specific interactions with phytopathogens. in many cases, plants respond to pathogenic stimuli by adapting their hormone levels. here, the interaction between the phytopathogen rhodococcus fascians and one of its host plants, tobacco, was analyzed phenotypically and molecularly. to elucidate the basis of the cell division modulation and shoot primordia initiation caused by r. fascians, tobacco plant ... | 2001 | 11204782 |
| the plant pathogen rhodococcus fascians colonizes the exterior and interior of the aerial parts of plants. | rhodococcus fascians is a plant-pathogenic bacterium that causes malformations on aerial plant parts, whereby leafy galls occur at axillary meristems. the colonization behavior on nicotiana tabacum and arabidopsis thaliana plants was examined. independent of the infection methods, r. fascians extensively colonized the plant surface where the bacteria were surrounded by a slime layer. r. fascians caused the collapse of epidermal cells and penetrated intercellularly into the plant tissues. the ons ... | 2001 | 11332724 |
| the fas locus of the phytopathogen rhodococcus fascians affects mitosis of tobacco by-2 cells. | the effect of rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco bright yellow-2 (by-2) cells was investigated. incubation of aphidicolin-synchronized by-2 cells with r. fascians cells specifically resulted in a broader mitotic index peak, an effect that was linked to an intact and expressed fas virulence locus. the obtained results pointed towards an effect of r. fascians on the prophase of mitosis. the relevance of these results to the ... | 2001 | 11248250 |
| kupffer cells of cirrhotic rat livers sensitize colon cancer cells to fas-mediated apoptosis. | metastasis of colorectal carcinomas rarely occurs in cirrhotic livers. our study investigated the influence of activated kupffer cells from cirrhotic rat livers on hepatic colonization and fasr-mediated apoptosis of colon cancer cells. a rat colon cancer cell line, rcn-9, was used to inoculate rat livers. treatment with conditioned media of kupffer cells isolated from ccl(4)-induced cirrhotic rat livers (cirrhotic kcm) significantly reduced the incidence of hepatic colonization of rcn-9 cells. i ... | 2001 | 11336480 |
| characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16s rdna sequences. | one hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophylic conditions from arctic (98 strains) and antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. by numerical analysis, 8 clusters, containing 2 to 59 strains, could be delineated, and 8 strains formed separate branches. five clusters contained strains from both poles, two minor clusters were confined to arctic ... | 2001 | 11403404 |
| the att locus of rhodococcus fascians strain d188 is essential for full virulence on tobacco through the production of an autoregulatory compound. | the ability of rhodococcus fascians strain d188 to provoke leafy gall formation on a variety of plant species is correlated with the linear plasmid pfid188, on which different pathogenicity loci were identified. the att locus affects the severity of symptom development on tobacco, whereas the fas locus is essential for virulence. to gain insight into the function of the att locus, sequence and expression analyses were performed. the att locus contains nine open reading frames homologous to argin ... | 2001 | 11679063 |
| apoptosis in oral lichen planus. | apoptotic cell death may be a contributory cause of basal cell destruction in oral lichen planus (olp). therefore. the purpose of this study was to investigate the rate of apoptosis in olp and the expression of two proteins (fasr and fasl) regulating this process. biopsies from 18 patients with histologically diagnosed olp were investigated, with comparison to normal oral mucosa of healthy persons. for visualisation of dna fragmentation, the tunel method was used. in order to characterise the in ... | 2001 | 11695759 |
| chromosomal locus that affects pathogenicity of rhodococcus fascians. | the gram-positive plant pathogen rhodococcus fascians provokes leafy gall formation on a wide range of plants through secretion of signal molecules that interfere with the hormone balance of the host. crucial virulence genes are located on a linear plasmid, and their expression is tightly controlled. a mutant with a mutation in a chromosomal locus that affected virulence was isolated. the mutation was located in gene vica, which encodes a malate synthase and is functional in the glyoxylate shunt ... | 2002 | 11807072 |
| reactive oxygen species modulate zn(2+)-induced apoptosis in cancer cells. | some recent evidence has suggested a protective role of zinc against cancer. the mechanism by which zinc exerts this action has not been defined and, in particular, it has not been clarified whether zinc may directly act on cancer cells and the molecular mechanisms involved in this effect. in this study, we examined the in vitro effect of zinc on the apoptosis of mouse ts/a mammary adenocarcinoma cells, studying the zinc-dependent modulation of the intracellular levels of reactive oxygen species ... | 2002 | 11864783 |
| the intermediate filament protein keratin 8 is a novel cytoplasmic substrate for c-jun n-terminal kinase. | keratins 8 (k8) and 18 are the primary intermediate filaments of simple epithelia. phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. a number of keratin in vitro and in vivo phosphorylation sites have been identified. one example is k8 ser-73, which has been implicated as an important phosphorylation site during mitosis, cell stress, and apoptosis. we show that k8 is strongly phosphorylated on ser-73 upon ... | 2002 | 11781324 |
| characterization of a coryneform isolate from fasciated mugwort (artemisia vulgaris). | the morphological and physiological features of coryneform isolated from fasciated mugwort (artemisia vulgaris) display many morphological similarities with the plant pathogenic corynebacteria, but differ from corynebacterium fascians in exhibiting motility albeit in only a small proportion of each cell population, and by its ability to hydrolyze asculin, its failure to produce urease and differences in pigmentation. the isolate appears to be related to corynebacterium fascians in its ability to ... | 2002 | 12019725 |
| leafy gall formation by rhodococcus fascians. | rhodococcus fascians infects a wide range of plants, initiating the formation of leafy galls that consist of centers of shoot amplification and shoot growth inhibition. r. fascians is an epiphyte but it also can establish endophytic populations. bacterial signals involved in symptom development initiate de novo cell division and shoot meristem formation in differentiated tissues. the r. fascians signals exert activities that are distinct from mere cytokinin effects, and the evidence points to a ... | 2001 | 11701858 |
| fly-attracting volatiles produced by rhodococcus fascians and mycobacterium aurum isolated from myiatic lesions of sheep. | bacterial strains isolated from the healthy breech mucosa and myiatic wounds of ewes were tested for their volatile production as fly attractants towards wohlfahrtia magnifica (diptera: sarcophagidae). cultures were studied as fly baits in field experiments, and strains performing with the best chemotropic effect were selected for further analysis. static and dynamic headspace samples from shaken cultures were examined by gas chromatography-mass spectrometry (gc-ms). strains identified as rhodoc ... | 2002 | 11777576 |
| pac1 and pacap expression, signaling, and effect on the growth of hct8, human colonic tumor cells. | the pituitary adenylate cyclase-activating polypeptide (pacap) type 1 receptor (pac1) is a heptahelical, g protein-coupled receptor that has been shown to be expressed by non-squamous lung cancer and breast cancer cell lines, and to be coupled to the growth of these tumors. we have previously shown that pacap and its receptor, pac1, are expressed in rat colonic tissue. in this study, we used polyclonal antibodies directed against the cooh terminal of pac1, as well as fluorescently labeled pacap, ... | 2002 | 12409223 |
| peripheral benzodiazepine receptor ligands reverse apoptosis resistance of cancer cells in vitro and in vivo. | the mitochondrial peripheral benzodiazepine receptor (mpbr) is involved in a functional structure designated as the permeability transition pore, which controls apoptosis. binding of fas/apo-1/cd95 triggers a prototypic apoptosis-inducing pathway. using four different human tumor cell lines (t-cell jurkat, neuroblastoma shep, osteosarcoma 143n2, and glioblastoma snb79 cell lines), all of which express cd95 and mpbr, we investigated the potential role of mpbr ligands in cd95-induced apoptosis. we ... | 2002 | 11888910 |
| investigations on rhizoplane actinobacteria communities of papyrus (cyperus papyrus) from an egyptian wetland. | wetlands have important global ecological functions, which include carbon storage and water interception. wetland contributes to the maintenance of regional and global biodiversity. though many important wetland ecological functions are based on microbial metabolism, we have scanty knowledge on microbial diversity in wetlands. plant rhizoplane habitats are considered to harbor highly diverse bacterial communities. most of the floating mats on river nile are dominated by papyrus (cyperus papyrus) ... | 2002 | 12512252 |
| water transformation in the media of biofilters controlled by rhodococcus fascians in treating an ethyl acetate-contaminated airstream. | biofilters do not provide much water for bacteria to grow. to use them efficiently and properly, it is essential to understand the kinetics of water transformation and to control moisture levels. this study aims to clarify whether the metabolism of microorganisms will improve the water-holding capacity of media or will intensify drying. this experiment was conducted in duplicate, that is, both with and without bacterial inoculation. both the constant water content mode and the declining water co ... | 2002 | 12022691 |
| virulence genes of the phytopathogen rhodococcus fascians show specific spatial and temporal expression patterns during plant infection. | the phytopathogenic bacterium rhodococcus fascians provokes shoot meristem formation and malformations on aerial plant parts, mainly at the axils. the interaction is accompanied by bacterial colonization of the plant surface and tissues. upon infection, the two bacterial loci required for full virulence, fas and att, were expressed only at the sites of symptom development, although their expression profiles differed both spatially and temporally. the att locus was expressed principally in bacter ... | 2002 | 12026179 |
| versatile persistence pathways for pathogens of animals and plants. | the glyoxylate cycle and the glycine cleavage system are part of conserved metabolic pathways involved in the chronic persistence of microorganisms in animal hosts. in the chromosome of the plant pathogen rhodococcus fascians, the vic locus has been identified as a region containing genes essential for persistence inside induced leafy galls. sequence analysis showed that this 18-kb locus is syntenic with chromosomal regions of mycobacterium species that encompass the 'persistence' loci of these ... | 2002 | 12419605 |
| characterization of the iron-regulated desa promoter of streptomyces pilosus as a system for controlled gene expression in actinomycetes. | background: the bioavailability of iron is quite low since it is usually present as insoluble complexes. to solve the bioavailability problem microorganisms have developed highly efficient iron-scavenging systems based on the synthesis of siderophores that have high iron affinity. the systems of iron assimilation in microorganisms are strictly regulated to control the intracellular iron levels since at high concentrations iron is toxic for cells. streptomyces pilosus synthesizes the siderofore d ... | 2003 | 12801423 |
| a large, mobile pathogenicity island confers plant pathogenicity on streptomyces species. | potato scab is a globally important disease caused by polyphyletic plant pathogenic streptomyces species. streptomyces acidiscabies, streptomyces scabies and streptomyces turgidiscabies possess a conserved biosynthetic pathway for the nitrated dipeptide phytotoxin thaxtomin. these pathogens also possess the nec1 gene which encodes a necrogenic protein that is an independent virulence factor. in this article we describe a large (325-660 kb) pathogenicity island (pai) conserved among these three p ... | 2005 | 15686551 |
| phenotypic alterations in arabidopsis thaliana plants caused by rhodococcus fascians infection. | arabidopsis thaliana (l.) heynh. plants were challenged with rhodococcus fascians at several developmental stages and using different inoculation procedures. a variety of morphological alterations was scored on the infected plants; some of them resembled phenotypes of a. thaliana mutants in their shoot apical meristem (sam) organization. infection with r. fascians did not affect sam organization in wild type nor in sam mutants. anatomical studies on the new organs formed after infection with r. ... | 2004 | 14968352 |
| culture collections and biochemistry. | this review describes the relationships and links between culture collections, which act as sources of genomes, transcriptomes, proteome, and metabolomes, and fields of research biochemistry that demand their support and help. in addition, the invaluable but not always rewarded efforts of these organizations as a source and conservator of organism diversity is discussed. biological waste-water treatment, ethanol as a non-finite source of energy, rhodococcus fascians as the source of a citrus-jui ... | 2003 | 12739105 |
| biosynthesis of auxin by the gram-positive phytopathogen rhodococcus fascians is controlled by compounds specific to infected plant tissues. | the role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. the phytopathogen rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. we show here for the first t ... | 2005 | 15746315 |
| can whole genome analysis refine the taxonomy of the genus rhodococcus? | the current systematics of the genus rhodococcus is unclear, partly because many members were originally included before the application of a polyphasic taxonomic approach, central to which is the acquisition of 16s rrna sequence data. this has resulted in the reclassification and description of many new species. hence, the literature is replete with new species names that have not been brought together in an organized and easily interpreted form. this taxonomic confusion has been compounded by ... | 2004 | 15449609 |
| plant-microbe interactions: identification of epiphytic bacteria and their ability to alter leaf surface permeability. | bacteria were either isolated from leaf surfaces of hedera helix or obtained from a culture collection in order to analyse their effect on barrier properties of isolated hedera and prunus laurocerasus cuticles. on the basis of the 16s rdna sequences the genera of the six bacterial isolates from hedera were identified as pseudomonas sp., stenotrophomonas sp. and achromobacter. water permeability of cuticles isolated from h. helix was measured before and after inoculation with the six bacterial st ... | 2005 | 15819920 |
| identification and cloning of genes involved in specific desulfurization of dibenzothiophene by rhodococcus sp. strain igts8. | the gram-positive bacterium rhodococcus sp. strain igts8 is able to remove sulfur from certain aromatic compounds without breaking carbon-carbon bonds. in particular, sulfur is removed from dibenzothiophene (dbt) to give the final product, 2-hydroxybiphenyl. a genomic library of igts8 was constructed in the cosmid vector plafr5, but no desulfurization phenotype was imparted to escherichia coli. therefore, igts8 was mutagenized, and a new strain (uv1) was selected that had lost the ability to des ... | 1993 | 16349035 |
| novel microbial epoxide hydrolases for biohydrolysis of glycidyl derivatives. | microbial isolates from biofilters and petroleum-polluted bioremediation sites were screened for the presence of enantioselective epoxide hydrolases active towards tert-butyl glycidyl ether, benzyl glycidyl ether, and allyl glycidyl ether. out of 270 isolated strains, which comprised bacteria, yeasts, and filamentous fungi, four were selected based on the enantioselectivities of their epoxide hydrolases determined in biotransformation reactions. the enzyme of aspergillus niger m200 preferentiall ... | 2005 | 16061300 |
| bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). | wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ... | 2006 | 16382282 |
| isolation of cis-zeatin from corynebacterium fascians cultures. | three cytokinin-active constituents have been isolated from the culture medium of corynebacterium fascians, a plant pathogen causing fasciation. one constituent is now identified as cis-zeatin [6-(4-hydroxy-3-methyl-cis-2-butenylamino)purine]. it represents the first unequivocal demonstration of the production of a zeatin isomer by a bacterial species. another constituent was identified earlier as 6-(3-methyl-2-butenyl-amino)purine. the third constituent, distinct, on sephadex lh-20 column chrom ... | 1973 | 16592126 |
| relationships between cytokinin production, presence of plasmids, and fasciation caused by strains of corynebacterium fascians. | cytokinin activity in the culture medium of four pathogenic strains of corynebacterium fascians varied from 168 to 0.4 mug of kinetin (6-furfurylaminopurine) equivalents per liter, as compared to 0.2 in an avirulent control. n(6)-isopentenyladenine was the predominant cytokinin in the medium of all five strains, and its increased production was correlated with the degree of pathogenicity; however, the virulent strains also produced 8-13 times more cis-zeatin [6-(4-hydroxy-3-methyl-cis-2-butenyla ... | 1980 | 16592766 |
| cytokinins in trna of corynebacterium fascians. | | 1968 | 16656786 |
| efficient transformation of the cephamycin c producer nocardia lactamdurans and development of shuttle and promoter-probe cloning vectors. | a high transformation efficiency (1 x 10 to 7 x 10 transformants per mug of dna) of nocardia lactamdurans lc411 was obtained by direct treatment of mycelium with polyethylene glycol 1000 and cesium chloride. a variety of vectors from streptomyces lividans, brevibacterium lactofermentum, rhodococcus fascians, and a nocardia (amycolatopsis) sp. were tested; transformants could be obtained only with vectors derived from an endogenous plasmid of the amycolatopsis sp. strain dsm 43387. vectors carryi ... | 1994 | 16349436 |
| rapid identification of cytokinins by an immunological method. | a method for rapid identification of bacterial cytokinins has been developed in which cultures are fed [(3)h]adenine, the cytokinins (including (3)h-labeled cytokinins) are isolated by immunoaffinity chromatography, and analyzed by hplc with on-line scintillation counting. analysis of agrobacterium tumefaciens strains showed that some produced primarily trans-zeatin, whereas others produced primarily trans-zeatin riboside. pseudomonas syringae pv savastanoi produced mixtures of trans-zeatin, dih ... | 1991 | 16668105 |
| transformation of rhodococcus fascians by high-voltage electroporation and development of r. fascians cloning vectors. | the analysis of the virulence determinants of phytopathogenic rhodococcus fascians has been hampered by the lack of a system for introducing exogenous dna. we investigated the possibility of genetic transformation of r. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid dna. electrotransformation in r. fascians d188 resulted in transformation frequencies ranging from 10/mug of dna to 10/mug of dna, depending on the dna concentration. the effects of diff ... | 1990 | 16348290 |
| rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil. | a gram-positive, aerobic, non-motile, mesophilic strain, designated yim 70056(t), was isolated from a forest soil sample in yunnan province, china. phylogenetic analysis based on 16s rrna gene sequences revealed that this isolate had less than 97.0 % similarity to any rhodococcus species with validly published names, with the exception of rhodococcus fascians (dsm 20669(t)), which was found to be its closest neighbour (98.9 % similarity). chemotaxonomic data, including peptidoglycan type, diagno ... | 2005 | 15879245 |
| cytokinins in corynebacterium fascians cultures: isolation and identification of 6-(4-hydroxy-3-methyl-cis-2-butenylamino)-2-methylthiopurine. | in addition to the four cytokinins, 6-(3-methyl-2-butenylamino)purine, 6-methylaminopurine and the cis and trans isomers of 6-(4-hydroxy-3-methyl-2-butenylamino)purine, reported earlier from our laboratories, three cytokinin-active fractions have been obtained from the aqueous medium of 6-day-old corynebacterium fascians cultures. one of these has been identified as 6-(4-hydroxy-3-methyl-cis-2-butenylamino)-2-methylthiopurine (2-methylthio-cis-zeatin, c-ms(2)io(6) ade).the elution volumes of the ... | 1976 | 16659759 |
| detection, isolation and partial characterization of an immunostimulating glycoprotein from rhodococcus fascians. | in a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, rhodococcus fascians, were able to induce cytokine release (tnf(alpha)) from mouse peritoneal macrophages. monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (mw>100 kda) glycoprotein. in addition, methods practicable for its biotechnological preparation and sev ... | 2006 | 16846838 |
| rhodostreptomycins, antibiotics biosynthesized following horizontal gene transfer from streptomyces padanus to rhodococcus fascians. | | 2008 | 18179219 |
| modulation of the hormone setting by rhodococcus fascians results in ectopic knox activation in arabidopsis. | the biotrophic actinomycete rhodococcus fascians has a profound impact on plant development and a common aspect of the symptomatology is the deformation of infected leaves. in arabidopsis (arabidopsis thaliana), the serrated leaf margins formed upon infection resemble the leaf phenotype of transgenic plants with ectopic expression of knotted-like homeobox (knox) genes. through transcript profiling, we demonstrate that class-i knox genes are transcribed in symptomatic leaves. functional analysis ... | 2008 | 18184732 |
| rhodococcus kyotonensis sp. nov., a novel actinomycete isolated from soil. | a polyphasic study was undertaken to establish the taxonomic position of an isolate, strain ds472(t), from soil in kyoto, japan. phylogenetic analysis, based on the 16s rrna gene sequences, revealed that this strain constitutes a new subline within the genus rhodococcus, with rhodococcus yunnanensis yim 70056(t) and rhodococcus fascians dsm 20669(t) as its nearest phylogenetic neighbours (98.2 and 97.8 % sequence similarity, respectively). dna-dna hybridization experiments revealed 36 and 29 % r ... | 2007 | 17766854 |
| rhodococcus fascians infection accelerates progression of tobacco by-2 cells into mitosis through rapid changes in plant gene expression. | * to characterize plant cell cycle activation following rhodococcus fascians infection, bacterial impact on cell cycle progression of tobacco by-2 cells was investigated. * s-phase-synchronized by-2 cells were cocultivated with r. fascians and cell cycle progression was monitored by measuring mitotic index, cell cycle gene expression and flow cytometry parameters. cell cycle alteration was further investigated by cdna-aflp (amplified fragment length polymorphism). * it was shown that cell cycle ... | 2007 | 17547674 |
| streptomyces turgidiscabies possesses a functional cytokinin biosynthetic pathway and produces leafy galls. | streptomyces turgidiscabies, a cause of potato scab, possesses a mobilizable pathogenicity island containing multiple virulence genes and a cytokinin biosynthetic pathway. these biosynthetic genes are homologous and collinear with the fas operon in rhodococcus fascians. reverse-transcriptase polymerase chain reaction of s. turgidiscabies demonstrated that all six genes were transcribed in oat bran broth with and without glucose, though transcription was partially repressed by glucose. the supern ... | 2007 | 17601163 |
| a common soil flagellate (cercomonas sp.) grows slowly when feeding on the bacterium rhodococcus fascians in isolation, but does not discriminate against it in a mixed culture with sphingopyxis witflariensis. | flagellates are very important predators on bacteria in soil. because of their high growth rates, flagellate populations respond rapidly to changes in bacterial numbers. previous results indicate that actinobacteria are generally less suitable than proteobacteria as food for flagellates. in this study, we investigated the growth of the flagellate cercomonas sp. (atcc 50334) on each of the two bacteria sphingopyxis witflariensis (alphaproteobacteria) and rhodococcus fascians (actinobacteria) sepa ... | 2008 | 18462400 |
| rhodococcus cercidiphylli sp. nov., a new endophytic actinobacterium isolated from a cercidiphyllum japonicum leaf. | an endophytic actinobacterium, designated yim 65003(t), was isolated from a surface sterilized leaf sample of cercidiphyllum japonicum collected from yunnan province, south-west china. the morphological and chemotaxonomic properties of the isolate were typical of members of the genus rhodococcus. analysis of the 16s rrna gene sequence revealed that the isolate was most closely related to rhodococcus fascians dsm 20669(t) (99.6%) and rhodococcus yunnanensis yim 70056(t) (99.0%). dna-dna hybridiza ... | 2008 | 18485653 |
| metabolic shift in the phytopathogen rhodococcus fascians in response to cell-free extract of infected tobacco plant tissues. | the phytopathogen rhodococcus fascians induces the development of leafy gall, which is considered to be its ecological niche. to obtain a view of the metabolic changes occurring in r. fascians during this process, an in vitro system was used where bacteria are grown in the presence of a leafy gall extract, a condition mimicking that found by the bacteria in infected plants. proteins of r. fascians grown for 24 h under these conditions were displayed by two-dimensional polyacrylamide gel electrop ... | 2009 | 19219499 |
| identification of rhodococcus fascians cytokinins and their modus operandi to reshape the plant. | decades ago, the importance of cytokinins (cks) during rhodococcus fascians pathology had been acknowledged, and an isopentenyltransferase gene had been characterized in the fas operon of the linear virulence plasmid, but hitherto, no specific ck(s) could be associated with virulence. we show that the ck receptors ahk3 and ahk4 of arabidopsis thaliana are essential for symptom development, and that the ck perception machinery is induced upon infection, underlining its central role in the symptom ... | 2009 | 19129491 |
| an integrated genomics approach to define niche establishment by rhodococcus fascians. | rhodococcus fascians is a gram-positive phytopathogen that induces shooty hyperplasia on its hosts through the secretion of cytokinins. global transcriptomics using microarrays combined with profiling of primary metabolites on infected arabidopsis (arabidopsis thaliana) plants revealed that this actinomycete modulated pathways to convert its host into a niche. the transcript data demonstrated that r. fascians leaves a very characteristic mark on arabidopsis with a pronounced cytokinin response i ... | 2009 | 19118125 |
| eternal youth, the fate of developing arabidopsis leaves upon rhodococcus fascians infection. | the phytopathogenic actinomycete rhodococcus fascians induces neoplastic shooty outgrowths on infected hosts. upon r. fascians infection of arabidopsis (arabidopsis thaliana), leaves are formed with small narrow lamina and serrated margins. these symptomatic leaves exhibit reduced tissue differentiation, display more but smaller cells that do not endoreduplicate, and accumulate in the g1 phase of the cell cycle. together, these features imply that leaf growth occurs primarily through mitotic cel ... | 2009 | 19118126 |
| occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland. | the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ... | 2009 | 19572487 |
| the tobacco ntann12 gene, encoding an annexin, is induced upon rhodoccocus fascians infection and during leafy gall development. | summary annexins are calcium-binding proteins that have been associated in plants with different biological processes such as responses to abiotic stress and early nodulation stages. until now, the implication of annexins during plant-pathogen interactions has not been reported. here, a novel plant annexin gene induced in tobacco by-2 cell suspension cultures infected with the phytopathogenic bacterium rhodococcus fascians (strain d188) has been identified. expression of this gene, called ntann1 ... | 2007 | 20507490 |
| biosurfactant production by halotolerant rhodococcus fascians from casey station, wilkes land, antarctica. | isolate a-3 from antarctic soil in casey station, wilkes land, was characterized for growth on hydrocarbons. use of glucose or kerosene as a sole carbon source in the culture medium favoured biosynthesis of surfactant which, by thin-layer chromatography, indicated the formation of a rhamnose-containing glycolipid. this compound lowered the surface tension at the air/water interface to 27 mn/m as well as inhibited the growth of b. subtilis atcc 6633 and exhibited hemolytic activity. a highly hydr ... | 2010 | 20135319 |
| analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry. | a sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete rhodococcus fascians. the cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (c18 and ion-exchange). the purified samples were further separated and analyzed by hplc-esi-ms/ms. this allowed to ach ... | 2010 | 20969996 |
| the phytopathogen rhodococcus fascians breaks apical dominance and activates axillary meristems by inducing plant genes involved in hormone metabolism. | summary rhodococcus fascians is a gram-positive bacterium that interacts with many plant species and induces multiple shoots through a combination of activation of dormant axillary meristems and de novo meristem formation. although phenotypic analysis of the symptoms of infected plants clearly demonstrates a disturbance of the phytohormonal balance and an activation of the cell cycle, the actual mechanism of symptom development and the targets of the bacterial signals are unknown. to elucidate t ... | 2006 | 20507431 |
| patterns of phenolic compounds in leafy galls of tobacco. | the chemical composition of ethanolic and aqueous extracts from leafy galls produced after infection of nicotiana tabacum l. plants with rhodococcus fascians was drastically changed compared to uninfected controls. chlorogenic acid was abundant both in uninfected and infected plants, but caffeic acid and another cinnamoyl analogue were new in leafy galls. the most pronounced product induced in leafy galls was identified as 7-o-methyl-6-o-beta-d-glucopyranosyl coumarin (7-methyl esculin). this is ... | 1997 | 19343412 |
| rhodococcus fascians impacts plant development through the dynamic fas-mediated production of a cytokinin mix. | the phytopathogenic actinomycete rhodococcus fascians d188 relies mainly on the linear plasmid-encoded fas operon for its virulence. the bacteria secrete six cytokinin bases that synergistically redirect the developmental program of the plant to stimulate proliferation of young shoot tissue, thus establishing a leafy gall as a niche. a yeast-based cytokinin bioassay combined with cytokinin profiling of bacterial mutants revealed that the fas operon is essential for the enhanced production of iso ... | 2010 | 20687806 |
| bacterial and plant signal integration via d3-type cyclins enhances symptom development in the arabidopsis-rhodococcus fascians interaction. | the phytopathogenic actinomycete rhodococcus fascians drives its host to form a nutrient-rich niche by secreting a mixture of cytokinins that triggers plant cell division and shoot formation. the discrepancy between the relatively low amount of secreted cytokinins and the severe impact of r. fascians infection on plant development has puzzled researchers for a long time. polyamine and transcript profiling of wild-type and cytokinin receptor mutant plants revealed that the bacterial cytokinins di ... | 2011 | 21459976 |
| ntann12 annexin expression is induced by auxin in tobacco roots. | ntann12, encoding a polypeptide homologous to annexins, was found previously to be induced upon infection of tobacco with the bacterium rhodococcus fascians. in this study, ntann12 is shown to bind negatively charged phospholipids in a ca(2+)-dependent manner. in plants growing in light conditions, ntann12 is principally expressed in roots and the corresponding protein was mainly immunolocalized in the nucleus. ntann12 expression was inhibited following plant transfer to darkness and in plants l ... | 2011 | 21543519 |
| Plant-derived auxin plays an accessory role in symptom development upon Rhodococcus fascians infection. | The biotrophic phytopathogen Rhodococcus fascians has a profound impact on plant development, mainly through its principal virulence factors, a mix of synergistically acting cytokinins that induce shoot formation. Expression profiling of marker genes for several auxin biosynthesis routes and mutant analysis demonstrated that the bacterial cytokinins stimulate the auxin biosynthesis of plants via specific targeting of the indole-3-pyruvic acid (IPA) pathway, resulting in enhanced auxin signaling ... | 2011 | 22181713 |
| effect of microorganisms on the plutonium oxidation states. | particular microbes from substrates at the low-level radioactive waste repository in the ignalina npp territory were exposed to (239)pu (iv) at low ph under aerobic conditions. pu(iii) and pu(iv) were separated and quantitatively evaluated using the modified anion exchange method and alpha spectrometry. tested bacteria bacillus mycoides and serratia marcescens were more effective in pu reduction than rhodococcus fascians. fungi paecillomyces lilacinus and absidia spinosa var. spinosa as well as ... | 2011 | 22112595 |
| a successful bacterial coup d'état: how rhodococcus fascians redirects plant development. | rhodococcus fascians is a gram-positive phytopathogen that induces differentiated galls, known as leafy galls, on a wide variety of plants employing virulence genes located on a linear plasmid. the pathogenic strategy consists in the production of a mixture of six synergistically acting cytokinins that overwhelm the plant's homeostatic mechanisms, ensuring the activation of a signaling cascade that targets the plant cell cycle and directs the newly formed cells to differentiate into shoot merist ... | 2010 | 21495844 |
| use of whole genome sequences to develop a molecular phylogenetic framework for rhodococcus fascians and the rhodococcus genus. | the accurate diagnosis of diseases caused by pathogenic bacteria requires a stable species classification. rhodococcus fascians is the only documented member of its ill-defined genus that is capable of causing disease on a wide range of agriculturally important plants. comparisons of genome sequences generated from isolates of rhodococcus associated with diseased plants revealed a level of genetic diversity consistent with them representing multiple species. to test this, we generated a tree bas ... | 2014 | 25237311 |
| glycogenformation by rhodococcus species and the effect of inhibition of lipid biosynthesis on glycogen accumulation in rhodococcus opacus pd630. | members of the genus rhodococcus were investigated for their ability to produce glycogen during cultivation on gluconate or glucose. strains belonging to rhodococcus ruber, rhodococcus opacus, rhodococcus fascians, rhodococcus erythropolis and rhodococcus equi were able to produce glycogen up to 0.2–5.6% of cellular dry weight (cdw). the glycogen content varied from 0.8% to 3.2% of cdw in cells of r. opacus pd630, which is a well-known oleaginous bacterium, during the exponential growth phase, w ... | 2010 | 21069909 |
| virulence quenching with a prenylated isoflavanone renders the malagasy legume dalbergia pervillei resistant to rhodococcus fascians. | the phytopathogenic actinomycete rhodococcus fascians induces leafy galls on a wide range of hosts, causing major economical losses in the ornamentals industry. although differences in the responsivity occur within species, no plant tested so far could be considered resistant to r. fascians strain d188 infection. here, we observed that members of the genus dalbergia, which belong to the fabaceae, did not develop leafy galls when challenged with r. fascians and we set out to unravel the mechanism ... | 2011 | 21332623 |