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current status of treatment of pneumonia.proper treatment of pneumonia is dependent upon a correct diagnosis. pneumonia may be due to infectious agents, allergic phenomena, or chemical causes. treatment regimens are outlined for the various types of pneumonia--pneumococcal, staphylococcal, fungal, and pneumonia due to gram-negative and anaerobic gram-negative bacilli, to blastomyces dermatitidis, and to the parasite pneumocystis carinii. in discussing current concepts of treatment, several well-known methods are emphasized, as well as ...1975792
the metabolism of cyclohexanol by acinetobacter ncib 9871.acinetobacter ncib 9871 was isolated by elective culture on cyclohexanol and grows with this compound as sole source of carbon. it displays a restricted growth spectrum, being unable to grow on a wide range of alternative alicyclic alcohols and ketones. cyclohexanol-grown cells oxidize the growth substrate at a rate of 230 mul of o2/h per mg dry wt with the consumption of 5.65 mumol of o2/mumol substrate. cyclohexanone is oxidized at a similar rate with the consumption of 4.85 mumol of o2/mumol. ...19751261
studies of nocardia pellegrino sn 5108 pigment mutants: reasons for differences in pigmentation (author's transl).yellow and white mutants of the strains nocardia pellegrino sn 5108 r have been isolated. regarding their morphological and physiological properties, the mutants are identical with the wild type bacteria with the exception of their pigmentation and lipid composition. however, the pigment composition (number, rf-values and spectra of the pigment components) of the yellow mutant is identical with that of the wild type; as a consequence, the modified pigmentation of the yellow mutant cannot be expl ...19763925
multiple forms of cyclohexanone oxygenase from nocardia globerula cl1.the cyclohexanone 1,2-monooxygenase of nocardia globerula cl1 exists as two electrophoretically distinct forms. these are present in crude cell extracts and are not artifacts of enzyme purification or electrophoresis. they have been separated in mg amounts by preparative polyacrylamide gel electrophoresis and shown to have essentially identical kinetic, spectral and physical characteristics. they do differ in ph-activity profile and temperature stability. whether or not they are conformational i ...19764312
chemistry of the ansamycin antibiotics. 197611155
comparative studies on cholesterol oxidases from different sources.1. comparison of the characteristics of cholesterol oxidases from different sources was made by a new polarographic method for measurement of the oxygen-consumption rate. 2. a ph optimum of 7.0 was observed for cholesterol oxidases isolated from nocardia and brevibacterium, ph 5.0 for the enzyme from schizophyllum and ph 7.5 for the enzyme from streptomyces. 3. in the system used in the present study, ca2+ and mg2+ had no effect on these enzyme activities. on the other hand, the schizophyllum en ...197611914
purification and properties of an unusual nitrilase from nocardia n.c.i.b. 11216. 197612045
dynamics of pyridine nucleotide coenzymes in nocardia cells. 197714180
further observations on an ether-o-oxidase, formerly called alkyl etherase, from liver tissue.1. the microsomal enzyme from liver previously called an "etherase" is now described more accurately as an ether-o-oxidase. it has been investigated further to free it from the membranes in aqueous solution and to try to define its physiological substrate. 2. after a variety of attempts with detergents, etc., the enzyme was obtained in impure solution from precipitation with 35-45% (nh4)2so4 solution after a short digestion at room temperature. 3. when a suitably reinforced the enzyme in solutio ...197717745
microbial metabolism of aromatic nitriles. enzymology of c-n cleavage by nocardia sp. (rhodochrous group) n.c.i.b. 11216.1. an organism utilizing benzonitrile as sole carbon and nitrogen source was isolated by the enrichment-culture technique and identified as a nocardia sp. of the rhodochrous group. 2. respiration studies indicate that nitrile degradation proceeds through benzoic acid and catechol. 3. cell-free extracts of benzonitrile-grown cells contain an enzyme that catalyses the conversion of benzonitrile directly into benzoic acid without intermediate formation of benzamide. 4. this nitrilase enzyme was pur ...197721655
mycetoma in somalia - results of a survey done from 1959 to 1964.the pasteur institute studied 103 mycetoma patients in somalia between 1959 and 1964. grains were seen in 94 of them and this, added to cultural features, allowed the diagnosis of 60 pathogens as follows: 44 madurella mycetomi, 1 leptosphaeria senegalensis, 7 pyrenochaeta romeroi (or madurella grisea), 3 allescheria boydii, 1 fusarium sp., 3 neotestudina (zopfia) rosatii, and 1 unidentified; 34 were actinomycetes: 24 streptomyces somaliensis, 4 actinomadura madurae, 3 a. pelletieri and 3 nocardi ...197723657
change in the composition and properties of humic acids under the action of microorganisms of the genus nocardia. 197825290
origin of adifferentiated variants of streptomyces roseoflavus in submerged culture.adifferentiated nocardia-like variants (nv) of streptomyces roseoflavus var. roseofungini, similar in their cultural characteristics with the variant 1-68 described elsewhere (nikitina, 1968; kalakoutskii a. nikitina, 1976), can be obtained not only in the course of growth of the parent culture on a solid medium containing fructose in the form of secondary colonies, but also during submerged cultivation with stirring. interruptions in aeration do not affect accumulation of the cells of such vari ...197826856
screening for ph-dependent alpha-l-arabinofuranosidases in various representative strains of actinomycetales. 197827906
changes in the composition and properties of humic acids brought about by the action of microorganisms of the genus nocardia.the results of an investigation of the influence of microorgansims of the genus nocardia on changes in the composition and properties of humic acids are presented. according to these data, the elemental composition and ir spectra showed different effects on the structure of humic acids after utilization of these compounds as a nutrient source for three and six months by microorganisms.197833724
growth of nocardia rhodochrous on acetylene gas.soil sediment enrichment cultures yielded a coryneform bacterium capable of growing in a mineral salts solution with acetylene gas as its only source of carbon and energy. based on morphological and physiological traits as well as on cell wall analysis, the bacterium was characterized as a strain of nocardia rhodochrous. maximal growth rates (generation time 2.7 to 3.0 h) on acetylene were obtained at 5 to 20% acetylene, 25 to 40% oxygen, ph 7.0 and 26 to 28 degrees c. yields (grams of dry cells ...197937235
rabbit b spleen lymphocytes and t helper cells. i. responsiveness to mitogens of b cell subpopulations of different sedimentation velocities and subpopulations bearing or lacking fcgamma receptors.the response to anti-allotype (anti-ab4), nocardia water soluble mitogen (nwsm), pneumococcal polysaccharide type iii (sss iii), and human fc fragments of various purified and unfractionated rabbit spleen cell populations was determined in terms of 3h-thymidine up-take. b cells were isolated either from untreated suspensions of spleen cells or from suspensions from which adherent and phagocytic cells were removed. the purification factor was greater than the enhancement of 3h-thymidine uptake by ...197939964
[interferon induced in the hamster by subunits of "nocardia": essay of protection against rabies (author's transl)].lipid-free cells of nocardia opaca and a cell extract (nwsm) are interferon inducers in syrian hamsters: peak interferon appears 2 h after injection as compared to 8 h in the case of interferon induced by inactivated ndv virus. the interferon induced by nocardia product is inactivated by a treatment for 30 min at 56 degrees c or 24 h at ph 2: ndv-induced interferon is more stable under these conditions. hamsters inoculated with nwsm before and after infection with rabies virus are partially prot ...197943690
[steroid-transforming enzymes from microorganisms. ix. affinity chromatographic preparation and studies of the apoenzyme from a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca].from the flavoenzyme, 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase of nocardia opaca, prosthetic group and apoenzyme were separated quantitatively by means of affinity chromatography in the presence of 2 m (nh4)2 at ph 3.0. subsequently the apoenzyme was eluted from affinity matrix by 0.01 m phosphate buffer, ph 8.0, whereas under these conditions the intact enzyme could not be eluted. the whole enzyme activity applied could be restored by incubation of the eluted apoenzyme with fad. the bi ...197944397
[steroid-transforming enzymes from microorganisms. xi. reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from nocardia opaca].highly purified preparations of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca have been investigated in both types of reactions: 1.2-dehydrogenation of the 4-en-3-oxo-derivative and 1.2-hydrogenation of the 1.4-dien-3-oxo-derivative. it was not possible to separate the hydrogenating activity from the dehydrogenating activity by affinity chromatography, disc electrophoresis, sds-electrophoresis, and isoelectric focusing techniques. the pure enzyme preparation is discus ...197944773
the effect of fixation procedures on the electron density of polysaccharide granules in nocardia corallina. 197551094
the adaptation of fluorescence microscopy for nocardia asteroides: a preliminary report.the fluorescence microscope is extremely effective in revealing the presence and morphology of nocardia asteroides. this has been effectively demonstrated when the auramine fluorochrome was decolourized with 1% h2so4 and the interval of decolourization was limited to two minutes and thirty seconds. when nocardia asteroids was seeded in sputum found to be negative for acid-fast organisms and in greatly diluted quantities after one hour intervals, its presence was still revealed with the flouresce ...197553968
[morphological tinctorial and biochemical properties of mycobacteria and nocardia]. 197657615
superoxide dismutase from mycobacterium species, strain takeo.superoxide dismutase from mycobacterium species, strain takeo, has been purified to homogeneity as judged by disc gel electrophoresis and ultracentrifugation. the enzyme was found to have a molecular weight of approximately 61 500 by sedimentation equilibrium and to contain manganese by atomic absorption and electron spin resonance spectra. the amino acid composition was also determined. the enzyme was considerably stable to the treatment with sodium dodecyl sulfate; unless incubating at 80 degr ...197658647
catechol 1,2-dioxygenase from acinetobacter calcoaceticus: purification and properties.procedures for the purification of catechol 1,2-dioxygenase from extracts of acinetobacter calcoaceticus strain adp-96 are described. the purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. the enzyme contained 2 g-atoms of iron per mol of protein. the enzyme had a broad substrate specificity and catalyzed the oxidation of catechol, 4-methylcatechol, 3-methylcatechol, and 3-isopropyl catechol. the activity of the enzyme was inhibited by heavy meta ...197658860
studies on the biodegradation of nonionic surfactants applied in the polyester fiber industry. i. activated sludge bacteria degrading the surfactants.the paper presents characteristics of 76 strains of bacteria capable of utilizing nonionic surfactants cirrasol fp, cirrasol sf 200 and cirrasol tcs as the source of carbon. the strains were isolated from two activated sludges adapted to the purification of wastes containing the above compounds at concentration 150--200 mg/l. the isolated strains belonged to the genera: achromobacter, alcaligenes, arthrobacter, flavobacterium, mycobacterium, nocardia, pseudomonas and xanthomonas. with load 0.11 ...197662497
bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties.cells of pseudomonas putida np, pseudomonas species (ncib 9816), and a nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (nad+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. similarly, cells of a strain of p. putida biotype a, when grown either on toluene or benzene vapors, were found to contain a dehydrogenase that oxidized dihydrodiols of aromatic hydrocarbons with cis ste ...197662750
[erythrasma of the nails].we have studied 17 cases of pseudomycotic onychoses. this condition is frequently seen in the toe nails with the clinical aspect of hyperkeratosis of the nail bed and onycholysis. the giemsa stained preparations have been performed with the clinical material previously heated in a 40% ohk solution and washed out with distilled water by centrifugation. the microorganism has similar or identical characteristics to those of the agent of erythrasma of the folds. its morphology and physiological char ...197665505
a comparative study of the 'rhodochrous' complex and related taxa by delayed-type skin reactions on guinea pigs and by polyacrylamide gel electrophoresis.cell extracts prepared by ultrasonic disruption of 17 strains of the 'rhodochrous' complex and related taxa were compared by polyacrylamide gel electrophoresis and for immunologic relatedness, by skin test reactions. two organisms, jensenia canicruria and nocardia calcarea, gave similar gel patterns and skin test reactions, and are considered to be identical. extracts of nocardia rubra showed a strong antigenic relationship with those of three nocardia pellegrino organisms (n325, n324 and n420) ...197770505
57co-bleomycin for brain scan diagnosis of intracranial lesions. 197775263
induction of interferon synthesis in mice by fractions from nocardia.three fractions of nocardia, nocardia water-soluble mitogen (nwsm), nocardia water-soluble mitogen pellet (nwsmp), and the cell wall peptidoglycan, which are mitogenic for b lymphocytes, were able to induce circulating interferon in mice, nwsmp and nwsm being the most active. the peak of interferon appeared about 2 h after injection. the interferon induced by nswmp and nwsm was acid stable and antigenically related to viral interferon, as shown by neutralization with antibodies directed against ...197875845
the metabolism of 1-phenylethanol and acetophenone by nocardia t5 and an arthrobacter species. 197877775
immunochemical relatedness among ribosomes of mycobacteria, nocardia and corynebacteria [proceedings]. 197880189
bacterial decomposition of synthetic 14c-labeled lignin and lignin monomer derivatives.nocardia sp. which was isolated from soil is capable of degrading synthetic lignin and utilizing its monomer derivatives. decomposition was monitored by measuring the 14co2 evolved and o2 consumed, when the bacterium was grown on a medium containing specifically 14c-labeled ligning or monomer phenolic compounds as major carbon source. the time course of the 14co2 release and o2 uptake indicates a significant depolymerization and utilization of lignin by the nocardia sp.197880922
the use of fluorescent and special staining techniques in the aspiration of nocardiosis and actinomycosis.this report outlines the techniques and illustrates the utility of special stains and fluorescent microscopy when applied to aspiration cytology material. this procedure allowed quick and accurate diagnosis of opportunistic infectious organisms, specifically nocardiosis and actinomycosis.197883771
evaluation of mycobacteriology laboratories: the acid-fast smear.in a proficiency testing program, slides bearing acid-fast bacilli were sent to laboratories in new york state. the most reliable results were obtained in laboratories using the ziehl-neelsen method. cold staining procedures were less reliable. the auramine-rhodamine fluorescent stain, which produced many false positives, should be confirmed by the ziehl-neelsen procedure. the testing program described can be used to determine whether laboratories perform the acid-fast stain effectively.197884804
cellular basis of regulation of expression of idiotype. ii. immunity to anti-mopc-460 idiotype antibodies increases the level of anti-trinitrophenyl antibodies bearing 460 idiotypes.the antibody response of balb/c mice to trinitrophenyl (tnp)-levan or tnp-nocardia water-soluble mitogen (nwsm) includes a small but significant fraction of antibodies which share idiotypes (id) with the dinitrophenyl (dnp)- and tnp-binding myeloma protein mopc-460. active immunization of balb/c mice with mopc-460 or passive administration of anti-460-id antibodies suppresses the 460-id+ component of the anti-tnp response. by contrast, active immunization of balb/c with anti-460-id antibodies or ...197985684
polyphosphate- and atp-glucose phosphotransferase activities of nocardia minima. 197989794
microbial conversion of ethylbenzene to 1-phenethanol and acetophenone by nocardia tartaricans atcc 31190.a culture of nocardia tartaricans atcc 31190 was capable of catalyzing the conversion of ethylbenzene to 1-phenethanol and acetophenone while growing in a shake flask culture with hexadecane as the source of carbon and energy. this subterminal oxidative reaction with ethylbenzene appears not to have been previously reported for nocardia species. when n. tartaricans was grown on glucose as its source of carbon and energy and ethylbenzene was added, no subsequent production of 1-phenethanol or ace ...197993878
immunological cross-reactivity of pathogenic nocardia and related organisms. 197994563
mycetoma caused by nocardia asteroides.during the last 5 years six cases of mycetoma have been referred to the razi hospital, thehran. nocardia asteroides was found to be the causative agent in one case only. five of the patients were males, all being rice farmers, and one was a female. all of them belonged to northern iran which has a mediterranean climate. treatment of the condition still remains unsatisfactory and ineffective and amputation may have to be performed as a last resort.197897414
microbial degradation of [c14c]polystyrene and 1,3-diphenylbutane.microbial degradation of [beta-14c]polystyrene and 1,3-diphenylbutane, a compound structurally representing the smallest repeating unit of styrene (dimer), was investigated in soil and liquid enrichment cultures. degradation rates in soil, as determined by 14co2 evolution from applied [14c]polystyrene, varied from 1.5 to 3.0% for a 4-month period. although relatively low, these percentages were 15 to 30 times greater than values previously reported. enrichment cultures, containing 1,3-diphenylbu ...197898222
antitumor activity of squalene-treated cell-wall skeleton of nocardia rubra in mice. 1978103768
immunological method to differentiate between antigens of tubercle bacilli, other mycobacterial species, and non-acid-fast bacteria.sera from rabbits immunized with sonicates of mycobacterium bovis bcg were passed through an immunoadsorbent made of a soluble bcg extract to make partially purified antibodies to bcg. these antibodies were in turn used to prepare an immunoadsorbent through which the bcg extract was passed. the partially purified antigenic material was radiolabeled and subjected to electrophoresis in acrylamide gels. one of the radiolabeled fractions isolated (bcg-c) was found to bind to antibodies to bcg and h3 ...1978103823
cross-reactions between mycobacteria. ii. crossed immunoelectrophoretic analysis of soluble antigens of bcg and comparison with other mycobacteria.cross-reactions between mycobacterium bovis bcg and various other mycobacteria, nocardia asteroides, corynebacterium pyogenes and listeria monocytogenes were studied by incorporating antibodies against these bacteria in the intermediate gel of a crossed immunoelectrophoretic system with bcg antigen and anti-bcg antibodies. in the bcg reference system forty-four distinct antigenic components were recorded, of which thiryt-three cross-reacted with mycobacterium tuberculosis, twenty-five with m. av ...1979106465
[enzymes of intermediary metabolism in coryneform bacteria].enzymes of the intermediate metabolism were studied in ten strains of corynebacterium-like organisms belonging to the genera arthrobacter, brevibacterium, corynebacterium and nocardia. all of these were found to contain enzymes of the glycolytic pathway, and nine strains among ten had dehydrogenases of the pentose phosphate shunt. the activity of enzymes of the citric acid cycle was low: alpha-ketoglutarate dehydrogenase was not found in arthrobacter, corynebacterium, brevibacterium linens and n ...1979108525
decomposition of ddt and its analogs by soil microflora.a search was made for active cultures that break down ddt. more than 600 microorganisms were isolated from soils treated for a long time with ddt and from accumulating cultures with ddt or its analogs as the carbon source and with ddt or its analogs and supplementary carbon sources. cultures capable of degrading ddt under conditions of cometabolism were found among them. it was shown that the dechlorination of the trichloromethyl group of ddt was accomplished by a large number of microorganisms ...1978109130
immunodiffusion studies of ribosomes in classification of mycobacteria and related taxa.ribosomal preparations consisting of crude ribosomes (cr), 30s subunits (30s) and 16s core particles (16s) from four strains of the species mycobacterium bovis (bcg), mycobacterium fortuitum, mycobacterium phlei and mycobacterium smegmatis were analyzed by immunodiffusion technique for taxonomical purposes. the ribosomal preparations tested contained several interspecies cross-reacting precipitinogens. the number of precipitinogens demonstrated at the homologous reactions was generally larger th ...1979109401
microbiological transformations of nabilone, a synthetic cannabinoid.a screening program was conducted to find microorganisms that modify the synthetic cannabinoid nabilone. after purification, the products from three cultures were analyzed by spectral methods to determine their chemical structures. an optically active 9s-hydroxy-6ar,10ar-trans cannabinoid was isolated from a culture of an unidentified soil bacterium designated a24007. from bacillus cereus cultures were isolated a 9s,6'-dihydroxy-6ar,10ar-trans cannabinoid, a 9s-hydroxy-6'-keto-6ar,10ar-trans can ...1979114111
microimmunodiffusion test for nocardiosis.sera from 71 patients with culturally proven nocardiosis were tested for precipitins against a pool of nocardia asteroides and n. brasiliensis culture filtrates and against antigens from the supernatant of homogenized n. asteroides cells. a human nocardiosis case serum was used as a reference. sera from 56 of the 71 cases were reactive with either the culture filtrate antigen, the homogenate antigen, or both antigens, resulting in an overall sensitivity of 79%. sera from 35 of the patients (49%) ...1979114533
[diauxotrophic properties of microorganisms assimilating c2--c4 hydrocarbons].diauxotrophic properties of bacteria assimilating gaseous hydrocarbons c2--c4 and other complex organic substances but not methane were studied. if the medium contained two substrates (hydrocarbon+carbohydrate), the non-growing cells of the strains did not display diauxotrophic properties. in the phase of exponential growth, oxygen-containing carbon sources and then gaseous hydrocarbons were assimilated, i.e. diauxia was observed. if a microbial association containing an obligate methylotroph a ...1979116113
isolation of acid-fast organisms from surgical specimens.a total of 204 surgical specimens positive for acid-fast organisms yielded 169 strains of mycobacterium tuberculosis, 15 of which were isolated by middlebrook 7h-9 broth medium only. mycobacteria other than m. tuberculosis were isolated from 33 specimens, and nocardia asteroides was isolated from 2 specimens.1979118985
[growth of nocardio- and coryneform bacteria on media with low molecular weight alcohols]. 1979119140
[carboxylation enzymes of coryneform bacteria].the enzymes of carbon dioxide heterotrophic fixation were studied in six strains of coryneform bacteria belonging to the genera arthrobacter, brevibacterium, corynebacterium and nocardia. all of the strains were found to contain pep (phosphoenolpyruvate) carboxylase (ec 4.1.1.31), nadp or nad dependent malic enzymes (ec 1.1.1.38--40). pyruvate carboxylase (ec 6.4.1.1) was found only in three strains of coryneforms: brevibacterium ammoniagenes, corynebacterium aquaticum and nocardia erythropolis. ...1979119147
in vivo activation of murine peritoneal macrophages by nocardia water soluble mitogen (nwsm).in vitro nwsm has been shown to be a b cell mitogen and a polyclonal activator. although nwsm has been also shown to be adjuvant active in vivo, motogenicity and polyclonal activation are not observed when this fraction has been administered to mice. in contrast, the experiments reported here demonstrate that after in vivo administration several effects of nwsm on mouse peritoneal macrophages can be observed: nwsm was able to induce an important increase in the ability of peritoneal macrophages ...1979121200
[plea for medical mycology, "step-child" of our doctrine and research]. 1975125248
immunoadjuvant activities of cell walls, their water-soluble fractions and peptidoglycan subunits, prepared from various gram-positive bacteria, and of synthetic n-acetylmuramyl peptides.1. the cell walls from some 20 species of gram-positive bacteria, with only few exceptions, were found to be definitely adjuvant-active in both stimulation of increased serum antibody levels and induction of delayed-type hypersensitivity to ovalbumin when administered to guinea pigs in the form of a water-in-oil emulsion. 2. by the use of various cell wall lytic enzymes, the immunoadjuvant principles were solubilized with the full retention of adjuvant activities observed with the cell walls of ...1975126564
drug eruption associated with sulfonamide treatment of vertebral osteomyelitis in a dog.drug eruption was diagnosed in a 4-year-old german shepherd dog being treated with sulfonamides for vertebral osteomyelitis due to infection with nocardia spp. the eruption was characterized by generalized, pruritic, eczematous dermatitis. diagnosis was based on a history of repeated exposure to sulfonamides, skin biopsy findingd, and clinical recovery on cessation of sulfonamide therapy.1976132414
a study of 3891 cases of mycoses in the tropics.4103 cases suspected of mycoses were analysed as to sex, age and site of disease and 3891 were proved cases. this group formed 50% of total mycoses or 13-93% of all dermatoses recorded in the government general hospital, madras, during the period of study. there were 66-26% adult female, 27-6% adult male and 6-14% were below 13 years. dermatophytoses were found in 73-5%; the other common diseases were tinea versicolor (17-68%) and candidiasis (12-43%). multiple sites of involvement or more than ...1976134458
[nocardie and their problems (author's transl)]. 1977145910
[isolation of actinomadura from soils on selective media with kanamycin and rifampicin]. 1978146454
[new species of the genus nocardia and its antibiotic properties].a culture of a new species of nocardia, i.e. n. indigoensis producing an antibiotic close to celicomycins was isolated from a soil sample of kazakhstan plated on the selective medium with kanamycin. by a number of chemical properties and biological activity the antibiotic differed from celicomycins. probably it is a new natural substance.1978147645
activation of human b lymphocytes. ix. modulation of antibody production by products of activated macrophages.human monocytes, after in vitro activation by mixed lymphocyte culture (mlc) supernatants produce a monokine (mk) that enhances the plaque-forming cell (pfc) response of pokeweed mitogen (pwm)-stimulated human b lymphocytes. technical conditions and kinetics of mk production were established. irradiation of monocytes (5000 rads) does not abolish mk production but heat-killed cells are unable to release the factor. highly t cell-depleted monocyte populations still produced the pfc-enhancing facto ...1978149173
[bioconversion of steroids in mutants of nocardia restrictus].nocardia restrictus grows quickly on synthetic media containing different carbonated substrates (steroids, organic acids). the control of growth parameters of this microbial species allowed the development of the production and selection methodology for mutants unable to grow on androst-4ène-3,17-dione. a stable mutant convert androst-4-ène-3,17-dione in perhydroindan propionic acid without addition of any degradation inhibitor in the medium.1978153186
[isolation of an autotrophic variant of nocardia opaca].a hydrogen-oxidizing autotrophic actinomycete, strain z-766, was isolated from the neuston of a marsh near moscow. the organism was identified as nocardia opaca z-766 according to its morphologo-physiological properties. the spectrum of organic compounds used by the strain, its cultural characteristics and growth are similar to those of the monotypical autotrophic microorganism n. opaca 1b described earlier. repeated isolation of the autotrophic variant of n. opaca z-766 suggests that the abilit ...1979155775
opportunistic infection in children. iii. in the normal host. 1975171360
production of 13beta-alkyl-3-methoxy-8,14-seco-1,3,5(10),9(11)-gonatetraen-14beta-ol-17-ones and 13beta-alkyl-3-methoxy-8,14-seco-1,3,5(10),9(11)-gonatetraen-17alpha-ol-14-ones by microbial enzymes.for the purpose of producing hydroxy-keto-seco-steroids in which hydroxyl group is attached to a carbon atom having the r-configuration, numerous biochemically active microorganisms were tested without any success. the hydroxysteroid oxidoreductase enzymes of the investigated bacterial, yeast and fungal strains were suitable only for the production of 17beta-ol-14-one and 14alpha-ol-17-one derivatives. the required compounds were prepared by combinations of enzymatic reactions with chemical redu ...1975179287
a comparative analysis of the ultrastructure of hydrocarbon-oxidizing micro-organisms.the ultrastructure of a variety of micro-organisms was compared after growth on hydrocarbon and non-hydrocarbon substrates. hydrocarbon-grown organisms were characterized by the presence of intracellular electron-transparent inclusions which in many cases appeared membrane-bound. with one exception, non-hydrocarbon-grown organisms did not contain electron-transparent inclusions. insignificant amounts of poly-beta-hydroxybutyric acid were produced by the hydrocarbon-grown micro-organisms. after g ...1976181527
the adjuvant effect of microbial products on the immune response. 1976185941
the fifth a.j. kluyver memorial lecture delivered before the netherlands society for microbiology on october 9th, 1975, at the delft university of technology, delft. the physiology of hydrogen bacteria. 1976185955
the mannophosphoinositides of nocardia asteroides.the phospholipid of n. asteroides has been investigated. it was found to contain phosphatidyl ethanolamine, cardiolipin, phosphatidyl inositol and a family of mannophosphoinositides. dimannophosphoinositides with 3 and 4 moles of fatty acid per phosphate residue represented the major glycophospholipids besides small amounts of other more glycosylated mannophosphoinositides.1976186296
the effect of cyclic amp on catabolite repression of isocitrate lyase in nocardia salmonicolor (ncib9701). 1976186557
genetic factors infuencing mouse type-c rna virus induction by naturally occurring b cell mitogens.the present studies demonstrate that xenotropic type c virus is efficiently released in response to lipopolysaccharide by spleen cells of a wide variety of inbred mouse strains. lipopolysaccharide-mediated virus release primarily involves b lymphocytes and is in part genetically determined. virus release can also be efficiently stimulated by other naturally occurring b cell mitogens, including nocardia water soluble mitogen, and ppd. the evidence indicates that these agents act synergistically w ...1977190319
the complementary role of computerized axial transmission tomography and radionuclide imaging of the brain.computerized axial transmission tomography and radionuclide imaging are complementary procedures, and the following recommendations are made as to their use. where there is no real clinical suspicion of intracranial disease, either modality can be used for "rule out" screening; the choice can frequently be made on the basis of which modality is cheaper or more quickly available. it should be remembered that "quicker" is often "cheaper". total cost is determined, not only by the cost of the proce ...1977193193
mannitol oxidation in two micromonospora isolates and in representative species of other actinomycetes.mannitol kinase and mannitol-1-phosphate dehydrogenase activities were detected in two micromonospora isolates. the presence of these enzyme activities indicates that mannitol is catabolized first to mannitol-1-phosphate and then to fructose-6-phosphate. mannitol-oxidizing enzymes were also surveyed in representative species of four other genera of actinomycetes. mannitol-1-phosphate dehydrogenase was detected in cell-free extracts of streptomyces lactamdurans. in contrast, cell-free extracts of ...1977194534
alterations in the phospholipid composition of nocardia polychromogenes during growth.the major phospholipid classes of nocardia polychromogenes were quantitated at different stages of the growth cycle. significant differences were observed both in the total lipid phosphorus per g (dry weight) of cells, and in the relative percentages of individual phospholipids. the total amount of lipid-phosphorus increased throughout the growth cycle. cardiolipin and phosphoinositides contents increased with significant decrease in phosphatidyl ethanolamine and unknown phospholipids.1977200456
[fluctuations in the level of cyclic amp and activities of adenylate cyclase and cyclic-amp phosphodiesterase in synchronous cultures of the prokaryote nocardia restricta (author's transl)]. 1978207351
antigenicity of mannophosphoinositides of nocardia. 1978210124
[cancer immunotherapy with bcg cell-wall skeleton (author's transl)]. 1978211546
periodic cyclic amp uptake by synchronously grown cells of nocardia restricta and arthrobacter globiformis. 1978212312
effect of cyclic nucleotides, isoproterenol and cholera toxin on dna synthesis triggered by mitogens.the effect of cyclic nucleotides, isoproterenol and cholera toxin on phytohaemagglutinin (pha), concanavalin a (con a) and anti-allotype-induced rabbit lymphoid cell proliferation was examined. cholera toxin in concentrations ranging from 10(-8) microgram to 1 microgram per culture inhibited dna synthesis, triggered by pha, con a and nocardia water-soluble mitogen (nwsm). it had the opposite effect on stimulation with antibodies directed against allotypic specificities of the immunoglobulin ligh ...1978212823
relapse of pneumonia caused by cytomegalovirus in two recipients of renal transplants.two cases of relapse of pneumonia caused by cytomegalovirus (cmv) in recipients of renal transplants were studied. between episodes of pneumonia, both patients recovered completely as judged by x-ray clearing and by resolution of fever and respiratory symptoms. cmv was isolated during each episode of pneumonia from lung tissue, pleural fluid, or buffy coat cultures. serologic studies revealed a rise in titer of antibody, confirming recent infection with cmv during the first epidose of pneumonia ...1979220337
the effects of mitogens on the expression of epstein-barr virus antigens in human lymphoid cell lines.treatment of human lymphoblastoid cells with either phytohemagglutinin (pha), concanavalin a, staphylococcus protein a, or polyinosinic acid-polycytidylic acid, in combination with 5-iodo-2' deoxyuridine (iudr) markedly increased the expression of epstein-barr virus (ebv) early antigen (ea) relative to iudr alone. such treatment did not, however, modify the production of virus capsid antigen in any of the lymphoid cell lines tested. the effect of pha on ea induction in raji cells was not accompa ...1979221589
[bronchopulmonary nocardiosis (author's transl)]. 1979227074
catabolic pathways of coryneforms, nocardias, and mycobacteria. 1979227323
cytostatic activity of peripheral blood monocytes against bronchogenic carcinoma cells in patients with lung cancer.cytostatic acitivity of peripheral blood monocytes against cultured cell lines of bronchogenic carcinoma was examined in patients with lung cancer. cytostatic activity in lung cancer patients with neither augmented nor suppressed as compared with that of controls such as normal healthy persons, patients with malignancies other than lung cancer, and patients with benign respiratory diseases. there was no correlation between the cytostatic activity of monocytes and the advance of clinical stages o ...1979229054
powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil.agar media made with 0.4% colloidal chitin plus mineral salts and adjusted to ph 8.0 was superior to four other commonly used media for the isolation and enumeration of actinomycetes from water samples. more actinomycetes developed on chitin agar, and the development of bacteria and fungi was suppressed. frozen and vacuum-dried chitin from aqueous colloidal suspensions was finely divided and gave results comparable to those obtained with media prepared from colloidal suspensions.1975234719
volume, adherence properties, membrane antigens and mitogen responsiveness of rabbit lymphoid cell subpopulations.subpopulations of rabbit spleen cells which respond to t and b mitogens, respectively, can be distinguished by sedimentation velocity in the earth's gravitational field. t cell subpopulations which differed in their responsiveness to con a and to pha could be identified by differences in adherence properties and by their sensitivity to complement mediated cell kill with rtla-antiserum.1975234909
penicillin acylase (bacterial). 1975237220
[distribution of pyrimidine blocks in the dna of brevibacterium linens, arthrobacter globiformis, nocardia corallina and nocardia rubra].the nucleotide composition and the frequency of pyrimidine blocks were studied in dna of the following bacteria: brevibacterium linens (weignamm, 1910) breed, 1953; arthrobacter globiformis (conn, 1928) conn et dimmick, 1947; nocardia corallina (bergey et al., 1923) waksman et henrici, 1948; nocardia rubra (krassilnikov, 1949) waksman et henrici, 1948. these organisms are classed by some microbiologists as mycobacteria (the mycobacteriaceae family) while other authors regard them as representati ...1975241002
granulocyte transfusion therapy in children.a prospective study of 45 granulocyte transfusions in children using continuous flow centrifugation is reported. during 13 episodes of proven or presumed infection, only two children failed to show a favorable response to granulocyte transfusion. the neutropenic child shows a significantly increased absolute granulocyte count one hour after transfusion. the granulocyte counts at one hour after transfusion are inversely proportional to the child's size. a child with chronic granulomatous disease ...1977265630
pulmonary nocardiosis associated with primary nocardial infection of the oral cavity.a case of pulmonary nocardiosis associated with primary nocardial infection of the oral cavity in a compromised host is presented. nocardia asteroides, an aerobic, gram-positive, branching, filamentous fungus, was demonstrated in the sputum and in pathologic specimens from gingival sulci stained by gram's method and the acid-fast method kinyoun. the organism was identified in cultures made on sabouraud's glucose agar. marked clinical improvement was noted when the patient received high dosage of ...1978272602
[case of nocardiasis of the submaxillary region and neck]. 1979290068
macrophage dependency of t-lymphocyte mitogenesis by nocardia rubra cell-wall skeleton.the mitogenic activity of the cell-wall skeleton (cws) of nocardia rubra on purified splenic t-cells (thymus-derived lymphocytes) was investigated. n. rubra cws showed remarkable mitogenic activity on normal spleen cells of c57bl/6j mice at concentrations ranging from 10 to 100 microgram per milliliter of culture medium, while, on purified splenic t-cells, n. rubra cws did not act as an mitogen at any concentration. however, mitogenic activity of n. rubra cws on t-cells was restored if purified ...1977304149
human b cell differentiation. i. immunoglobulin synthesis induced by nocardia mitogen.nocardia water-soluble mitogen (nwsm) is a mitogen which activates human b lymphocytes even in the absence of t cells. tonsil and peripheral blood lymphocytes cultured in the presence of nwsm gave rise to large numbers of cells containing cytoplasmic immunoglobulins of igm, iga or igg classes. the number of plasma cells appeared to be elevated as early as day 3 of culture and peaked on day 5 or 7. the igm response preceded the development of cells containing other ig classes, and was found to re ...1978306922
mitogenic analysis of murine b-cell heterogeneity.the b-cell mitogens lipopolysaccharide (lps), nocardia water-soluble mitogen (nwsm), and dextran sulfate (dxs) react with different subpopulations of b lymphocytes. selective in vitro killing of cells responding to either lps or nwsm has little effect on the in vitro response to the other mitogen, although the response to dxs is reduced in both cases. if, after selective in vitro killing, cells are injected into irradiated mice for 2-3 wk before measuring their in vitro mitogen responses, the sa ...1978307586
beta-lactamase production and resistance to beta-lactam antibiotics in nocardia.although ampicillin has been suggested as a useful agent for the treatment of nocardiosis in man, little is known regarding the presence of beta-lactamase in nocardia or its possible role in determining resistance to ampicillin and the other beta-lactam antibiotics. we have evaluated 55 isolates of nocardia for susceptibility to five beta-lactam antibiotics and for the presence of beta-lactamase. nocardia were resistant to penicillin g, cloxacillin, and cefazolin, but 27 and 62% were susceptible ...1978310280
production of beta-lactamase by non-streptomyces actinomycetales.supernatants and whole cells from fermentation broths of micromonospora, nocardia, oerskovia, and other genera of the actinomycetales were examined for the presence of beta-lactamase activity by using the chromogenic cephalosporin 87/312. nearly 60% of the 250 isolates examined produced detectable levels of beta-lactamase. all enzyme preparations were active over a range of ph values from 6.5 to 8.2, with maximum activity occurring between 7.0 and 7.8. the preparations varied in their stability ...1979311614
mitogen-activated b-cell blasts reactive to more than one mitogen.one of three splenic b cells of 2- to 3-mo-old c57bl/6j or c3h/tif mice are activated either by lipopolysaccharide (lps) or by lipoprotein (lp) to grow and to mature to igm-secreting cells. when mitogen-activated blast cells, after a 2-d activation period, are separated from nonactivated, small cells by velocity sedimentation, and no mitogen is readded, the blasts do not continue to grow but do continue to secrete igm for several days. when the homologous mitogen is added for restimulation, the ...1979311808
human b cell differentiation. ii. suppression by t cells of t-dependent and t-independent plasma cell maturation.in vitro human plasma cell generation induced by both t-dependent (pwm) and t-independent (nwsm) mitogens was found to be suppressed by peripheral blood lymphocytes preincubated with human aggregated igg. t cells, but not b lymphocytes, were able to mediate the suppressive activity; since aggregated (fab)'2 fragments were found unable to generate suppressor cells, it was concluded that the suppressor cell was a t lymphocyte bearing fcgamma receptors. these cells appeared to be largely radiosensi ...1979312853
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