| genetic heterogeneity within mycobacterium fortuitum complex species: genotypic criteria for identification. | a 1.5-kb segment of the dna that encodes 16s rrna was amplified by polymerase chain reaction, and 880 nucleotide positions were determined from each of the described biovariants of mycobacterium fortuitum. signature sequences which allow rapid identification of m. fortuitum strains at the biovariant level are described. our data demonstrate a close phylogenetic relationship between mycobacterium senegalense and m. fortuitum and indicate that the described biovariants of m. fortuitum represent ge ... | 1992 | 1280641 |
| distribution of a novel mycolic acid in species of the genus mycobacterium. | we found that mycobacterium porcinum atcc 33776t (t = type strain) contains a new kind of mycolic acid with a methoxy group at the omega-1 position. this mycolic acid was identified by comparing it with the previously described methoxymycolic acids. the patterns of mycolic acid methyl esters from 418 strains belonging to 44 species of mycobacteria were studied by using thin-layer chromatography. in addition to m. procinum atcc 33776t, representative strains of m. porcinum, mycobacterium fortuitu ... | 1991 | 1883713 |
| on the aetiology of bovine farcy in the sudan. | fifteen strains of the agent of bovine farcy were isolated from lymph nodes of affected cattle. quantitative analyses of mycolic acids revealed values that allowed the assignment of these strains to the genus mycobacterium. the organisms bore a greater resemblance to mycobacterium farcinogenes than to mycobacterium senegalense. | 1986 | 3806052 |
| identification of actinomycetes isolated from cases of bovine farcy in the sudan. | thirteen isolates from the lymph nodes of cattle with bovine farcy were examined for biochemical and physiological properties known to differentiate mycobacterium farcinogenes, mycobacterium senegalense and nocardia farcinica. comparative immunodiffusion studies were performed on all of the test strains, and whole-organism methanolysates from a single representative strain were examined by two-dimensional thin-layer chromatography. the results of the biochemical, chemical, physiological and sero ... | 1985 | 3923733 |
| mycobacterium senegalense from bovines in eastern nigeria. | detailed characteristics of three mycobacterial strains sharing important properties of mycobacterium senegalense are described. their physiological properties were compared with those of a typical m. senegalense strain described by chamoiseau (1979), six strains of m. senegalense and one typical strain of m. fortuitum from the culture collection of institute of microbiology, rutgers. the three nigerian strains exhibited minor variations in their physiological properties when compared with other ... | 1985 | 3932294 |
| equivalence of mycobactins from mycobacterium senegalense, mycobacterium farcinogenes and mycobacterium fortuitum. | mycobactins were isolated from five strains designated mycobacterium farcinogenes and a similar number designated mycobacterium senegalense following growth under conditions of iron-limitation. these lipid-soluble iron-chelating compounds were characterized by a combination of thin-layer and high-performance liquid chromatography. the mycobactins from both the slow-growing m. farcinogenes and the rapidly-growing m. senegalense strains proved impossible to differentiate both from each other and f ... | 1985 | 4045424 |
| immunodiffusion analyses of mycobacterium farcinogenes, mycobacterium senegalense and some other mycobacteria. | thirty-one strains of mycobacterium farcinogenes and m. senegalense were analysed together with twenty-five strains representing ten mycobacterial species. the comparative immunodiffusion technique was employed, using two reference systems, one for m. farcinogenes and the other for m. senegalense. the results indicate that the slow growing m. farcinogenes and the fast growing m. senegalense strains are closely related and can not be separated into two clearly distinct clusters. both species were ... | 1983 | 6409983 |
| classification of mycobacterium farcinogenes and mycobacterium senegalense by immunodiffusion and thin-layer chromatography of long-chain components. | comparative immunodiffusion studies and thin-layer chromatographic analyses of whole-organism acid methanolysates were performed on 37 strains of mycobacterium farcinogenes, mycobacterium senegalense and nocardia farcinica. the latter were clearly distinguished from the mycobacteria in containing a single mycolic acid methyl ester and showing more precipitinogens with nocardial than with mycobacterial and rhodococcal reference systems. the distribution of precipitinogens showed that m. farcinoge ... | 1982 | 6811691 |
| numerical classification of mycobacterium farcinogenes, mycobacterium senegalense and related taxa. | sixteen strains designated mycobacterium farcinogenes, fifteen mycobacterium senegalense, and ten nocardia farcinica were, together with strains of mycobacterium and nocardia, subjected to numerical phenetic analyses using 96 unit characters. the data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering achieved using the unweighted average linkage algorithm. cluster composition was not markedly affected by the coefficient used or by test error ... | 1983 | 6875513 |
| identification of mycobacteria from animals by restriction enzyme analysis and direct dna cycle sequencing of polymerase chain reaction-amplified 16s rrna gene sequences. | two methods, based on analysis of the polymerase chain reaction-amplified 16s rrna gene by restriction enzyme analysis (rea) or direct cycle sequencing, were developed for rapid identification of mycobacteria isolated from animals and were compared to traditional phenotypic typing. bactec 7h12 cultures of the specimens were examined for "cording," and specific polymerase chain reaction amplification was performed to identify the presence of tubercle complex mycobacteria. combined results of sepa ... | 1993 | 7508456 |
| thin-layer chromatographic analysis of glycolipids and mycolic acids from mycobacterium farcinogenes, mycobacterium senegalense and related taxa. | seventy-nine representative strains of mycobacterium farcinogenes, mycobacterium fortuitum, mycobacterium peregrinum and mycobacterium senegalense were analysed by thin-layer chromatography for diagnostic glycolipid and mycolic acid patterns. on the basis of glycolipid patterns the 16 m. senegalense strains were assigned to four groups (i, ii, iii and iv); one strain received as m. farcinogenes had glycolipids that corresponded to those given by the m. senegalense group iii strain. representativ ... | 1993 | 8219506 |
| structures of the glycopeptidolipid antigens of two animal pathogens: mycobacterium senegalense and mycobacterium porcinum. | the structures of the major glycolipid antigens of two animal pathogens mycobacterium senegalense and mycobacterium porcinum were elucidated by a combination of fast-atom bombardment mass spectrometry, nuclear magnetic resonance spectroscopy, chemical analyses and radiolabeling experiments. five glycoconjugates belonging to the class of c-mycoside glycopeptidolipids were characterized in each species. they shared with those recently described in m. peregrinum the same unusual distribution of the ... | 1993 | 8354291 |
| differentiation of mycobacterium senegalense from related non-chromogenic mycobacteria using pyrolysis mass spectrometry. | twenty-six representative strains of mycobacterium chelonae, mycobacterium fortuitum, mycobacterium peregrinum and mycobacterium senegalense were compared by curie point pyrolysis mass spectrometry. the m. chelonae and m. senegalense strains formed distinct groups. a third, relatively diffuse group, contained the m. fortuitum and m. peregrinum strains. these results, together with those from corresponding analyses, suggest that pyrolysis mass spectrometry provides a rapid and reliable way of dis ... | 1997 | 9060160 |
| novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16s-23s rrna gene spacer and restriction endonucleases. | a novel genus-specific pcr for mycobacteria with simple identification to the species level by restriction fragment length polymorphism (rflp) was established using the 16s-23s ribosomal rna gene (rdna) spacer as a target. panspecificity of primers was demonstrated on the genus level by testing 811 bacterial strains (122 species in 37 genera from 286 reference strains and 525 clinical isolates). all mycobacterial isolates (678 strains among 48 defined species and 5 indeterminate taxons) were amp ... | 2000 | 10699003 |
| mycobacterium septicum sp. nov., a new rapidly growing species associated with catheter-related bacteraemia. | rapidly growing mycobacteria are capable of causing several clinical diseases in both immunosuppressed and immunocompetent individuals. a previously unidentified, rapidly growing mycobacterium was determined to be the causative agent of central line sepsis in a child with underlying metastatic hepatoblastoma. four isolates of this mycobacterium, three from blood and one from the central venous catheter tip, were studied. phenotypic characterization, hplc and genetic analysis revealed that while ... | 2000 | 10758863 |
| differentiation between mycobacterium farcinogenes and mycobacterium senegalense strains based on 16s-23s ribosomal dna internal transcribed spacer sequences. | 16s ribosomal dna (rdna) and 16s-23s internal transcribed spacer rdna sequence analyses were performed on mycobacterium farcinogenes and m. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the m. fortuitum complex. m. farcinogenes and m. senegalense were indistinguishable in their 5"-end 16s rdna but showed both considerable interspecies spacer sequence divergence and a high level of intraspecie ... | 2002 | 11826003 |
| taxonomic variation in the mycobacterium fortuitum third biovariant complex: description of mycobacterium boenickei sp. nov., mycobacterium houstonense sp. nov., mycobacterium neworleansense sp. nov. and mycobacterium brisbanense sp. nov. and recognition of mycobacterium porcinum from human clinical isolates. | the mycobacterium fortuitum third biovariant complex (sorbitol-negative and sorbitol-positive) contains unnamed taxa first characterized in 1991. these organisms can cause respiratory infections, a spectrum of soft tissue and skeletal infections, bacteraemia and disseminated disease. to evaluate this group of organisms, clinical reference isolates and the type strains of m. fortuitum third biovariant complex sorbitol-negative (n = 21), m. fortuitum third biovariant complex sorbitol-positive (n = ... | 2004 | 15388725 |
| catheter-associated bacteremia by mycobacterium senegalense in korea. | rapidly growing mycobacteria is recognized as one of the causative agents of catheter-related infections, especially in immunocompromised hosts. to date, however, mycobacterium senegalense, which was known as the principal pathogen of bovine farcy, has not been reported in human infection. | 2005 | 16307688 |
| polyphasic characterization reveals that the human pathogen mycobacterium peregrinum type ii belongs to the bovine pathogen species mycobacterium senegalense. | mycobacterium peregrinum consists of two taxa: types i and ii. we evaluated 43 clinical type ii strains from throughout the united states. they were responsible for soft-tissue and bone infections, catheter-related infections, and possible pneumonitis. by carbohydrate utilization, they were indistinguishable from type i strains, being d-mannitol and trehalose positive. however, they had a distinct susceptibility pattern that included intermediate ciprofloxacin mics but low clarithromycin and dox ... | 2005 | 16333077 |
| mycobacterium senegalense tissue infection in a child after fish tank exposure. | the present report describes the first known case of an otherwise healthy child who developed a soft tissue infection due to mycobacterium senegalense - a pathogen usually found in east africa that is responsible for infecting various animals. the patient presented with nonhealing wounds after sustaining facial lacerations from the shattered glass of a fish tank. the patient responded well to scar revision and antibiotics, with no subsequent relapse. | 2011 | 22942887 |
| current perspectives on mycobacterium farcinogenes and mycobacterium senegalense, the causal agents of bovine farcy. | mycobacterium farcinogenes and m. senegalense are the causal agents of bovine farcy, a chronic, progressive disease of the skin and lymphatics of zebu cattle. the disease, which is prevalent mainly in sub-saharan africa, was in earlier times thought to be caused by nocardia farcinica and can be described as one of the neglected diseases in cattle. some aspects of the disease have been investigated during the last five decades but the major development had been in the bacteriological, chemotaxono ... | 2014 | 24876989 |
| epidemiology of nontuberculous mycobacteria in french polynesia. | as few data are available in the pacific countries and territories of the oceania region regarding nontuberculous mycobacteria,we retrospectively identified 87 such isolates from french polynesia from 2008 to 2013 by hybridization using dna-strip, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and partial rpob gene sequencing. partial rpob gene sequencing classified 42/87 (48.3%) isolates in the mycobacterium fortuitum complex, 28 (32.2%) in the mycob ... | 2015 | 26400787 |
| identification of different subtypes of rapid growing atypical mycobacterium from water and soil sources: using pcr-rflp using hsp65 and rrna 16s-23s genes. | nontuberculosis mycobacteria (ntm) are a diverse group of microorganisms that cause a variety of diseases in humans including skin, respiratory, and gastrointestinal tract infection. generally, ntm are classified into two categories: rapid (<7days) and slow growing (>7days). in this study, we aimed to investigate ntm frequency and prevalence in environmental samples. additionally, we tried to identify various subtypes of isolated rapid growing mycobacteria (rgm). | 2016 | 28043561 |
| epidemiology, pathology, immunology and diagnosis of bovine farcy: a review. | bovine farcy (which is caused by mycobacterium farcinogenes and mycobacterium senegalense) is a chronic suppurative granulomatous inflammation of the skin and lymphatics of cattle and is seen mostly in sub-saharan africa. it is not yet certain whether nocardia farcinica causes cutaneous nocardiosis (farcy) in animals that mimics bovine farcy. epidemiological data have steadily reported finding bovine farcy in adult cattle of the transhumance pastoralist tribes of the sahel and the sudanian savan ... | 2012 | 22341733 |
| draft genome sequence of mycobacterium peregrinum strain csur p2098. | mycobacterium peregrinum is a nonpigmented rapid growing nontuberculosis species belonging to the mycobacterium fortuitum group. the draft genome of m. peregrinum type i csur p2098 comprises 7,109,836 bp exhibiting a 66.23% g+c content, 6,894 protein-coding genes, and 100 predicted rna genes. its genome analysis suggests this species differs from mycobacterium senegalense. | 2015 | 26543113 |