Publications

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susceptibility of mycobacterium fallax to imipenem and twenty other antimicrobial agents. 19846594239
nontuberculous mycobacteria on ready-to-eat, raw and frozen fruits and vegetables.the consumption of fruits and vegetables is increasing worldwide because of the positive impact of these foods on human health. ready-to-eat, raw whole, and frozen fruits and vegetables were purchased from markets and examined for the presence of nontuberculous mycobacteria (ntm) using culture, real-time pcr (qpcr), and sequencing. using qpcr, mycobacterium sp. at 10(0) to 10(4) ge/g (genome equivalents per gram) was found in almost all of the 178 samples; members of the m. avium complex were fo ...201627497136
cyclopropanation of α-mycolic acids is not required for cording in mycobacterium brumae and mycobacterium fallax.the capacity to form microscopic cords (cording) of mycobacterium species has been related to their virulence. the compounds responsible for cording are unknown, but a recent study has shown that cording could be related to the fine structure of α-mycolic acids. this investigation attributes the need for a proximal cyclopropane in α-mycolic acids for cording in mycobacterium tuberculosis and mycobacterium bovis bcg and proposes cyclopropanases as good targets for new chemotherapeutic agents. as ...201222493302
hoyosella altamirensis gen. nov., sp. nov., a new member of the order actinomycetales isolated from a cave biofilm.a novel actinomycete, strain ofn s31(t), was isolated from a complex biofilm in the altamira cave, spain. a polyphasic study was carried out to clarify the taxonomic position of this strain. phylogenetic analysis with 16s rrna gene sequences of representatives of the genera corynebacterium, dietzia, gordonia, millisia, mycobacterium, nocardia, rhodococcus, segniliparus, skermania, tsukamurella and williamsia indicated that strain ofn s31(t) formed a distinct taxon in the 16s rrna gene tree that ...200919643882
mycobacterium insubricum sp. nov.five independent strains, isolated from clinical samples but probably not responsible for disease, revealed phenotypic and genotypic features that appeared to exclude their belonging to any of the recognized mycobacterium species. the strains, which are non-pigmented rapid growers, presented a cell-wall lipid pattern resembling those of mycobacterium brumae and mycobacterium fallax. sequencing of the 16s rrna, hsp65 and rpob genes and the 16s-23s rrna gene internal transcribed spacer (its) revea ...200919502346
structural elucidation of the predominant motifs of the major cell wall arabinogalactan antigens from the borderline species tsukamurella paurometabolum and mycobacterium fallax.tsukamurella paurometabolum and mycobacterium fallax are members of the suprageneric actinomycete group corynebacterineae that possesses a cell wall skeleton composed of a peptidoglycan to which an arabinogalactan is covalently attached. this polysaccharide is further modified by esterification with c60-c80 mycolic acid residues in mycobacteria and t. paurometabolum. however, m. fallax and t. paurometabolum produce polyenoic (up to six double bonds) mycolic acids whereas the most common type of ...200515761023
contribution of beta-lactamase production to the resistance of mycobacteria to beta-lactam antibiotics.mycobacterium fallax (m. fallax) is naturally sensitive to many beta-lactam antibiotics (mic < 2 microg/ml) and devoid of beta-lactamase activity. in this paper, we show that the production of the beta-lactamase of mycobacterium fortuitum by m. fallax significantly increased the mic values for good substrates of the enzyme, whereas the potency of poor substrates or transient inactivators was not modified. the rates of diffusion of beta-lactams through the mycolic acid layer were low, but for all ...19979136901
gas chromatography/tandem mass spectrometry as an analytical tool for the identification of fatty acids.structures of fatty acids present at very low quantities in mycobacteria are difficult to determine. a commonly used strategy is to introduce heteroatoms into functional groups by chemical means before subjecting them to gas chromatography/tandem mass spectrometry (gc/ms/ms) analysis. routinely used methods give very low abundance diagnostic ions leading to ambiguities in structural conclusions. gc/ms/ms associated with electron capture ionization of pentafluorobenzyl esters was used to study ve ...19957475983
isolation of the new species mycobacterium fallax in israel. 19846511342
structure determination of mycolic acids by using charge remote fragmentation.the collision-induced remote site fragmentation process of closed-shell ions, such as carboxylate anions, is a very potent analytical tool for the structural determination of fatty acids. this leads to an easy location of branch points, double bonds, cyclopropane rings and other functional groups. although corynomycolic acid mixtures from corynebacterium diphtheriae can be directly analyzed by negative-ion fast atom bombardment combined with collisionally activated decomposition spectra, mycolic ...19892504502
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