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mycobacterium cookii sp. nov.strains of a new type of slowly growing scotochromogenic mycobacterium were isolated repeatedly from sphagnum vegetation and surface water of moors in new zealand. these strains grew at 31 and 22 degrees c but not at 37 degrees c and possessed catalase, acid phosphatase, and arylsulfatase activities. they did not split amides, and most of them were susceptible to antituberculotic drugs. furthermore, they did not tolerate 0.1% naoh2 and 0.2% picric acid and did not grow on compounds used as singl ...19901697763
sensitisation of cattle to bovine and avian tuberculins with mycobacterium cookii. 19911746116
mycobacterium branderi sp. nov., a new potential human pathogen.a number of mycobacterial strains with similar growth characteristics, metabolic properties, and lipid compositions, which were previously placed in the helsinki group (e. brander, e. jantzen, r. huttunen, a. juntunen, and m.-l. katila, j. clin. microbiol. 30:1972-1975, 1992), were characterized by performing 16s rrna gene sequencing. of the 14 strains studied, 9 had a unique, previously undescribed sequence in the variable region of 16s rrna. these nine strains, all of which were isolated from ...19958590682
an is900-like sequence found in a mycobacterium sp. other than mycobacterium avium subsp. paratuberculosis.the insertion sequence is900 has been considered specific for mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) and has, therefore, been used as the target gene for diagnostic pcr of m. paratuberculosis. from a healthy dairy cow we have isolated and characterised a mycobacterium harbouring one copy of a sequence with 94% identity to is900 at the nucleic acid level. the isolate was shown to be related to mycobacterium cookii, as assessed by 16s rrna sequencing. strong amplificatio ...200212007816
gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria.cellular fatty acid analysis by glc is widely used in the species identification of mycobacteria. combining mycolic acid cleavage products with shorter cellular fatty acids increases the informative value of the analysis. a key has been created to aid in the identification of all currently known slowly growing environmental species. in this scheme, the species are classified into six categories, each characterized by a combination of fatty markers shared by those species. within each category, i ...200312676870
isolation of novel bacteria and actinomycetes using soil-extract agar medium.novel bacteria were discovered using an isolation technique consisting of (i) selection of microorganisms that grew on soil-extract agar medium, but not on conventional media, and (ii) detection of small microbial colonies with a microscope. three bacterial strains thus isolated were provisionally designated shinshu-th1, -th2, -th 3, and five actinomycete strains, shinshu-ms-01, -02, -03, -04, -05, respectively. sequence analysis of their 16s rdna showed that th1 had 95--96% homology with three ...200516233821
regrowth of potential opportunistic pathogens and algae in reclaimed-water distribution systems.a study of the quality of reclaimed water in treated effluent, after storage, and at three points in the distribution system of four plants in california, florida, massachusetts, and new york was conducted for 1 year. the plants had different treatment processes (conventional versus membrane bioreactor), production capacities, and methods for storage of the water, and the intended end uses of the water were different. the analysis focused on the occurrence of indicator bacteria (heterotrophic ba ...201020453149
mycobacteria are hidden endophytes in the shoots of rock plant [pogonatherum paniceum (lam.) hack.] (poaceae).a mycobacterium was isolated from micropropagated pogonatherum paniceum and identified as a close relative of mycobacterium cookii. the endophyte diversity in the shoots of potted and micropropagated p. paniceum plants was studied by culture-independent techniques. group- and strain-specific pcr demonstrated that the p. paniceum plants harboured the isolated mycobacterium strain as a minority. altogether 101 clones of the pcr products were sequenced. the shoots of potted p. paniceum plants harbo ...201023766233
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