| enzymatic synthesis of 2-o-alpha-d-mannopyranosyl-methyl-alpha-d-mannopyranoside by a cell-free particulate system of mycobacterium smegmatis. | a cell-free particulate enzyme preparation of mycobacterium smegmatis atcc 607 catalyzed the transfer of labeled mannose from gdp14c mannose to methyl-alpha-d-mannopyranoside (an exogenously added acceptor) to form a product that was characterized to be 2-o-alpha-d14c mannopyranosyl-methyl-alpha-d-mannopyranoside. this transmannosylase activity was specific for both the sugar nucleotide donor and methyl monosaccharide acceptor. the reaction was stimulated by the addition of various metal ions an ... | 1976 | 6051 |
| metabolism of triacylglycerol in mycobacterium smegmatis. | mycobacterium smegmatis cells incorporated 1-14coleic acid into triacylglycerols (tg) from the medium more rapidly than shorter chain fatty acids, caprilic and butyric acids. this incorporation was inhibited more strongly by 10(-3) m n-ethylmaleimide than by 10(-3) m kcn. 14ctg in the bacterial cells was utilized when the cells were in poor nutritional conditions, such as phosphate buffer (ph 7.0) containing oleic acid. accumulation of tg was observed in the cells at late stages of growth. digly ... | 1976 | 12154 |
| characterization of autolysins from mycobacterium smegmatis. | this study demonstrates, for the first time, the autolytic enzymes associated with mycobacterial cell walls. based on the release of radioactivity and ninhydrin-reactive material from isolated cell walls, it was shown that maximum activity occurs during the late log phase of growth and at a buffer ph of about 8.0. chemical analyses of autolytic digests of isolated cell walls indicated that at least three autolysins are active under the conditions used. these are n-glycolylmuramic acid-l-alanine ... | 1977 | 14109 |
| subunit composition and some properties of palmityl-coa-acp-transacylase of mycobacterium smegmatis. | | 1977 | 18217 |
| steroid transforming enzymes from microorganisms. iv. purification and cofactor requirement of the 4-ene-3-oxosteroid-5alpha-reductase from mycobacterium smegmatis. | | 1977 | 18639 |
| neutral lipid biosynthesis in mycobacterium smegmatis. | the biosynthesis of neutral lipids in mycobacterium smegmatis was studied using cell free extracts. maximum neutral lipid production was obtained when the reaction mixture (400 microliter) consisted of 0.25 m potassium phosphate buffer (ph 7.5), 0.125 mm oleoyl-coa, 3.75 mm sn-glycerol-3-p, 10 mm mgcl2 and 1.85 mg bovine serum albumin. no magnesium dependency for the acylation of sn-glycerol-3-p was observed. a slight stabilizing effect seemed to occur due to this ion. the enzyme phosphatidate p ... | 1977 | 20150 |
| acetyl-coa-dependent elongation of fatty acids in mycobacterium smegmatis. | an enzyme system of mycobacterium smegmatis catalyzing the elongation of medium-chain fatty acids with acetyl-coa was obtained free from de novo fatty acid synthetase by ammonium sulfate fractionation. the system was resolved by gel filtration and deae-cellulose chromatography into three fractions, all of which were required for reconstitution of the elongation activity. the three fractions were highly purified enoyl-coa hydratase, highly purified 3-hydroxyacyl-coa dehydrogenase, and a fraction ... | 1977 | 21175 |
| control mechanisms for fatty acid synthesis in mycobacterium smegmatis. | | 1977 | 21523 |
| role of malic enzyme in mycobacteria: part ii--purification & properties of malic enzyme from mycobacterium smegmatis. | | 1978 | 33117 |
| effect of polymethylpolysaccharides on the hydrolysis of palmitoyl coenzyme a by a thioesterase from mycobacterium smegmatis. | | 1979 | 40995 |
| inhibition of dihydrostreptomycin action on mycobacterium smegmatis by monovalent and divalent cation salts. | we have examined and compared the effects of monovalent and divalent cation salts on dihydrostreptomycin (dsm) action against mycobacterium smegmatis. the sauton synthetic liquid medium used was supplemented with test salts on the basis of ionic strength (mu). turbidimetric growth experiments showed that 0.02 m mgso(4) (mu = 0.08) prevented growth inhibition by 0.1 mug of dihydrostreptomycin per ml, but 0.02 m nacl (mu = 0.02) did not. however, at molarities equivalent to mu = 0.08, four monoval ... | 1975 | 50048 |
| inhibition of dihydrostreptomycin binding to mycobacterium smegmatis by monovalent and divalent cation salts. | to better understand salt antagonism of dihydrostreptomycin (dsm) action on mycobacterium smegmatis, the effects of monovalent and divalent cation salts on drug uptake were studied in relation to the lethal activity of dsm. in sauton liquid medium nacl, mgcl(2), and srcl(2) inhibited the initial instantaneous binding of [(3)h]dsm to the organism and suppressed secondary uptake. these data correlated well with the capacity of each salt to prevent the lethal activity of dsm. it was concluded that ... | 1976 | 56916 |
| localization of co-resistance to streptomycin, kanamycin, capreomycin, and tuberactinomycin in core particles derived from ribosomes of viomycin-resistant mycobacterium smegmatis. | | 1976 | 62857 |
| resistance relationships in mycobacterium smegmatis atcc 607 to phages sensitive or resistant to both chloroform and streptomycin sulphate. | four of eight mycobacteriophages did not form plaques after they were exposed to chloroform. phages sensitive to chloroform did not produce plaques when plated on media containing 1000 microgram/ml of streptomycin sulphate. the same concentration of dihydrostreptomycin sulphate did not interfere with plaque formation. mutants of mycobacterium smegmatis resistant to each of the eight phages were isolated. sensitivity or resistance to chloroform and streptomycin sulphate and phage resistant bacter ... | 1978 | 77894 |
| comparison of several culture media used for studies on mycobacteriophages. | the value of rva, n-1, 7h10, 7h11 and sauton's media for studies on mycobacteriophage infeciton and lysis of mycobacteria was assessed. experiments were made with mycobacteriophages bgi, bki, cri-3, g37, and lg, all of which lyse mycobacterium smegmatis strain 607b, and with mycobacteriophage ds6a which lyses mycobacterium tuberculosis strain h37rv. the methods involved "direct lysis", the measurement of "routine test dilutions" and counts of plaque-forming units. it was found that n-1, 7h10 and ... | 1978 | 96257 |
| studies on transfer rna from mycobacteria. | active preparations of trna and aminoacyl-trna synthetases have been isolated from exponentially growing cells of mycobacterium smegmatis and mycobacterium tuberculosis h37rv. though the aminoacyl-trna synthetases of older cells retain their activity, the trnas seem to undergo modification and show poorer activity. the mycobacterial enzyme preparations catalyse homologous and heterologous aminoacylation between trna from the two species (m. smegmatis and m. tuberculosis h37rv) or from escherichi ... | 1978 | 96929 |
| mycolic acids. a reinvestigation. | mycolic acids derived from the cell walls of mycobacterium bovis bcg, mycobacterium bovis bovinus i, mycobacterium smegmatis, and mycobacterium tuberculosis h37rv have been fractionated as their p-bromophenacyl esters by a two-step high performance liquid chromatographic procedure: 1) adsorption chromatography on 10-micrometer particle size silica gel, and 2) reverse phase partition chromatography on a 10-micrometer particle size support containing a c18 bonded phase. this procedure has resulted ... | 1978 | 97301 |
| acetylated methylmannose polysaccharide of streptomyces griseus. locations of the acetyl groups. | the positions of esterification of the 4 to 5 acetyl residues in the acetylated methylmannose-containing polysaccharide from streptomyces griseus have been established by the methyl replacement technique, wherein ester substituents are specifically replaced with methyl ether substituents. the newly incorporated methyl groups were distinguished from 3-o-methyl groups by the use of polysaccharide containing radioactively labeled endogenous methyl groups. the positions of methyl group localization ... | 1979 | 107171 |
| purification and properties of valyl-trna synthetase from mycobacterium smegmatis. | valyl-trna synthetase from mycobacterium smegmatis has been purified over 1200-fold by conventional techniques as well as affinity chromatography on valyl-aminohexyl sepharose columns. the purified preparation is homogeneous by electrophoretic and immunologic criteria. the enzyme is a tetramer of approximate molecular weight of 120,000, composed of a single type of subunit. the synthetase exhibited maximal activity between 35--40 degrees c and ph 6.8--7.0. the pure enzyme though stable for sever ... | 1979 | 109126 |
| immunodiffusion studies of ribosomes in classification of mycobacteria and related taxa. | ribosomal preparations consisting of crude ribosomes (cr), 30s subunits (30s) and 16s core particles (16s) from four strains of the species mycobacterium bovis (bcg), mycobacterium fortuitum, mycobacterium phlei and mycobacterium smegmatis were analyzed by immunodiffusion technique for taxonomical purposes. the ribosomal preparations tested contained several interspecies cross-reacting precipitinogens. the number of precipitinogens demonstrated at the homologous reactions was generally larger th ... | 1979 | 109401 |
| immunodiffusion studies of various structural preparations from mycobacterial cells. | various structures and other preparations from mycobacterial cells were analyzed by immunodiffusion. the preparations were obtained from four strains referred to the species mycobacterium bovis (bcg), mycobacterium fortuitum, mycobacterium phlei and mycobacterium smegmatis. they represented cell walls (cw), culture filtrates (cf), artificially disintegrated cell material (xp), protoplasms (pp), crude ribosomes (cr), ribosomal 50s subunits (50s), ribosomal 30s subunits (30s), ribosomal 16s core p ... | 1979 | 109402 |
| preparation of protoplasts and whole cell ghosts from mycobacterium smegmatis. | cell wall-deficient forms of mycobacterium smegmatis were produced in growth medium containing d-cycloserine and horse serum. these cells were transformed into protoplasts with edta and lysozyme. subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts). | 1979 | 119034 |
| alkaloids of thalictrum. xxii. isolation of alkaloids with hypotensive and antimicrobial activity from thalictrum revolutum. | sixteen alkaloids were characterized from thalictrum revolutum dc., namely; thalidasine, o-methylthalmethine, o-methylthalicberine, thalrugosaminine, thalicarpine, thalmelatine, pennsylvanine, palmatine, berberine, thalifendine, columbamine, jatrorrhizine, deoxythalidastine, thalphenine and magnoflorine. the structure of thairugosaminine (1) a bisbenzylisoquinoline type which was previously proposed on partial data was completely established, including the absolute configuration as s,s. thalphen ... | 1977 | 144834 |
| studies on mg2+-(ca2+-)activated adenosine triphosphatase from mycobacterium smegmatis cdc 46. | | 1978 | 147930 |
| enhancement of immunity against murine syngeneic tumors by a fraction extracted from non-pathogenic mycobacteria. | the data reported here demonstrate that a preparation extracted from nonpathogenic mycobacteria such as mycobacterium smegmatis and hereafter referred to as interphase material protected mice against ehrlich ascitic carcinoma, l-1210 leukemia, and another syngeneic lymphoid leukemia. furthermore, mice treated by this preparation were much less susceptible to endotoxins than when stimulated by bcg (bacillus calmette-guerin) or m. smegmatis cells. moreover, guinea pigs treated by interphase materi ... | 1975 | 171670 |
| altered ribosomes in antibiotic-resistant mutants of mycobacterium smegmatis. | two alleles for viomycin-capreomycin resistance (vic) in mycobacterium smegmatis affect ribosome structures. one (vica) affects a component of 50s subunits and the other (vicb) affects a component of 30s subunits. the locus for neomycin-kanamycin resistance (nek), which is linked to vica and vicb, affects a component of 30s subunits. although the erythromycin resistance locus (ery) is linked to vic and nek, no ribosomal alterations could be detected. mutations at the streptomycin locus (str) not ... | 1976 | 182073 |
| pathways of glucose catabolism in mycobacterium smegmatis. | glucose metabolism in mycobacterium smegmatis was investigated by the radiorespirometric method and by assaying for key enzymes of the major energy-yielding pathways. glucose is oxidized in this organism mainly through the embden-meyerhof-parnas pathway, irrespective of the carbon source used for growth. the pentose phosphate pathway plays only a minor role and its extent depends on the carbon source used for growth. enzymes of glycolytic and oxidative pathways were detected in cells grown on gl ... | 1976 | 184906 |
| localization of viomycin resistance in core particles derived from 30s ribosomal subunits of mycobacterium smegmatis. | | 1976 | 189750 |
| mannitol oxidation in two micromonospora isolates and in representative species of other actinomycetes. | mannitol kinase and mannitol-1-phosphate dehydrogenase activities were detected in two micromonospora isolates. the presence of these enzyme activities indicates that mannitol is catabolized first to mannitol-1-phosphate and then to fructose-6-phosphate. mannitol-oxidizing enzymes were also surveyed in representative species of four other genera of actinomycetes. mannitol-1-phosphate dehydrogenase was detected in cell-free extracts of streptomyces lactamdurans. in contrast, cell-free extracts of ... | 1977 | 194534 |
| alterations in lipid constituents during growth of mycobacterium smegmatis cdc 46 and mycobacterium phlei atcc 354. | phospholipids of mycobacterium phlei atcc 354 and mycobacterium smegmatis cdc 46 consist of cardiolipin, phosphatidyl ethanolamine, tri-acylated dimannophosphoinositide, tetra-acylated dimannophosphoinositide and tetra-acylated pentamannosphosphoinositide. a comparative study of lipid patterns of m. phlei atcc 354 and of m. smegmatis cdc 46 in relation to age of culture revealed higher total lipid level and increased activity of malate-vitamin k reductase, a phospholipid requiring enzyme, during ... | 1976 | 196159 |
| fatty acid synthetase activity in mycobacterium smegmatis. characterization of the acyl carrier protein-dependent elongating system. | mycobacterium smegmatis extracts contain two fatty acyl synthetase systems (brindley, d.n., matsumura, s. and bloch, k. (1966) nature 224, 666-669). one is the extensively studied multienzyme complex, (molecular weight 1.39 - 10(6)) which produces shorter c16 and c18) and longer (c24 and higher) fatty acids in a bimodal pattern. the second synthetase is acyl carrier-protein (acp) dependent and elongates the coa derivatives of c12 and longer chains. in contrast to the type i synthetase which also ... | 1977 | 196658 |
| identification of adenosine 3',5'-monophosphate in mycobacterium smegmatis. | cyclic adenosine 3',5'-monophosphate isolated from mycobacterium smegmatis cells was identified by thin-layer chromatography, stepwise conversion to adenosine 5'-monophosphate and adenosine, ultraviolet absorption spectrum, phosphate analysis, and detection by two relatively specific radioisotopic methods. | 1977 | 200600 |
| metabolism of cyclic amp in non-pathogenic mycobacterium smegmatis. | the intracellular concentration of cyclic amp reached a maximum in 3.5-day old cultures of mycobacterium smegmatis grown in the presence of glycerol as the main source of carbon. glucose-grown cells exhibited decreased cyclic amp levels at all stages of growth. when m. smegmatis cells were incubated with various metabolites, pyruvate increased whereas glucose, ctric acid, succinic acid and lactic acid decreased intracellular cyclic amp levels. no cyclic amp was detected in the incubation medium. ... | 1979 | 218515 |
| interactions between viomycin resistance and streptomycin resistance on ribosomes of mycobacterium smegmatis. | | 1979 | 228160 |
| interaction between 30 s ribosomal components in a viomycin resistant mutant of mycobacterium smegmatis. | a high level viomycin resistant mutant of mycobacterium smegmatis atcc 14468 (ac16) was analyzed genetically and biochemically in an attempt to understand the mechanisms of expression of high level viomycin resistance and co-resistance to kanamycin and streptomycin. genetic analysis has shown that at least three different genes (vicc, str, and kan) were involved in the phenotypic expression of drug resistance in ac16, and high level resistance to viomycin was due to interactions between the prod ... | 1979 | 228161 |
| studies on the mechanism of mycobacterium smegmatis l-lactate oxidase. 5-deazaflavin mononucleotide as a coenzyme analogue. | apo-lactic oxidase from mycobacterium smegmatis reconstituted with the deazaisoalloxazine analogue of fmn, 5-deazafmn, undergoes reduction by l-lactate but not catalytic reoxidation by o2. the rate of deazafmn-holo-enzyme reduction by substrate is 1.1 min-1. with l-[alpha-3-h]-lactate, direct tritium transfer to enzyme-bound deazafmn occurs during reduction. no evidence for a stable covalent lactate-deazafmn adduct has been obtained. the deaza-fmnh2-enzyme is reoxidized extremely slowly by o2, c ... | 1975 | 234460 |
| variations in the pathways of malate oxidation and phosphorylation in different species of mycobacteria. | mycobacterium tuberculosis h37rv, the slow-growing human pathogenic strain of tubercle bacilli and mycobacterium smegmatis and mycobacterium phlei, the fast-growing saprophytes, have shown variations regarding the type of dehydrogenase that initiates malate oxidation in the respiratory chain. m. tuberculosis h37rv is characterized by having a malate oxidase system (designated malnad pathway) in which malate oxidation is mediated by the nad+-dependent malate dehydrogenase (ec 1.1.1.37) but not by ... | 1975 | 234747 |
| iron transport in mycobacterium smegmatis: ferrimycobactin reductase (nad(p)h:ferrimycobactin oxidoreductase), the enzyme releasing iron from its carrier. | | 1975 | 237787 |
| purification, crystallization, and properties of d-ribose isomerase from mycobacterium smegmatis. | d-ribose isomerase, which catalyzes the conversion of d-ribose to d-ribulose, was purified from extracts of mycobacterium smegmatis grown on d-ribose. the purified enzyme crystalized as hexagonal plates from a 44% solution of ammonium sulfate. the enzyme was homogenous by disc gel electrophoresis and ultracentrifugal analysis. the molecular weight of the enzyme was between 145,000 and 174,000 by sedimentation equilibrium analysis. its sedimentation constant of 8.7 s indicates it is globular. on ... | 1975 | 240851 |
| alkaloids of thalictrum. xxi. isolation and characterization of alkaloids from the roots of thalictrum podocarpum. | thirteen alkaloids, hernandezine, thalidezine, n-desmethylthalidezine, isothalidezine, thalistyline, thalistyline methodiiodide, n-desemethylthalistyline, berberine, columbamine, jatrorrhizine, palmatine, thalifendine, magnoflorine and the artifact, 8-trichloromethyldihydroberberine were isolated from the roots of thalictrum podocarpum humb. in addition, oxyberberine and thaliglucinone were obtained in very minor amounts and identified by tlc. of these compounds, n-desmethylthalidezine and isoth ... | 1977 | 331006 |
| nitramino acids. synthesis and biological evaluation of 1-nitroproline, 1-nitropipecolic acid, and n-nitrosarcosine. | the n-nitro derivatives of secondary alpha-amino acids, viz., 1-nitroproline (1a) (l and d), 1-nitro-dl-pipecolic acid (2a), and n-nitrosarcosine (3a), were prepared by the oxidation of the corresponding nitrosamino acids with peroxytrifluoroacetic acid. these nitramino acids (1a-3a) were not active against escherichia coli, candida albicans, pseudomonas aeruginosa, or mycobacterium smegmatis, and 1a and 2a did not show mutagenic activity in a salmonella typhimurium ta-100 system, with or withou ... | 1977 | 338899 |
| interaction of mycobacterial polymethylpolysaccharides with paranaric acid and palmitoyl-coenzyme a: structural specificity and monomeric dissociation constants. | the long-chain polyenoic fatty acids alpha- and beta-paranaric acid form complexes with the 6-o-methylglucose polysaccharide from mycobacterium smegmatis as demonstrated by an enhanced fluorescence emission of the paranaric acid. this interaction is eliminated by digestion of the methylglucose polysaccharide with alpha-amylase and glucoamylase, which removes four hexose units from the nonreducing end of the chain. titration of the methylglucose polysaccharide with either paranaric acid isomer su ... | 1978 | 345276 |
| phage typing of mycobacteria using paper discs. | the suitability of phage-impregnated paper discs for the phage typing of mycobacteria was studied. the relevance of the routine test dilution, the volume of the phage used, the mode of incubation, and the effect of prolonged storage of phage-impregnated paper discs were considered. by using paper discs, each impregnated with one of 5 different mycobacteriophages (bg1, bk1, g37, cri-3 and lg) that lyse mycobacterium smegmatis 607b, it was determined that 100 x the routine test dilution in a volum ... | 1978 | 354442 |
| occurrence of 1-methyladenosine and absence of ribothymidine in transfer ribonucleic acid of mycobacterium smegmatis. | the minor base composition of mycobacterium smegmatis trna has been studied. thin-layer chromatographic patterns of a ribonuclease t2 digest of mycobacterial trna indicated the presence of appreciable amounts of 1-methyladenosine (which is commonly present only in eucaryotic trna), dihydrouridine, and 7-methylguanosine. ribothymidine was absent. the s-adenosylmethionine-dependent trna methylases of m. smegmatis catalyzed the formation of 1-methyladenosine when escherichia coli trna was used as a ... | 1979 | 374335 |
| acetylated methylmannose polysaccharide of streptomyces. | a polysaccharide composed of 3-o-methyl-d-mannose and d-mannose in a molar ratio of approximately 10:1 and containing 3 to 4 esterified acetyl residues has been isolated from streptomyces griseus. this acetylated methylmannose polysaccharide (ammp) is similar to the methylmannose polysaccharide (mmp) of mycobacterium smegmatis (gray, g. r., and ballou, c. e. (1971) j. biol. chem. 246, 6835-6842) in its size and composition, the absence of acidic or basic groups, and the lack of a reducing end. i ... | 1977 | 404290 |
| characterization of 3-o-methyl-d-mannose polysaccharide precursors in mycobacterium smegmatis. | | 1979 | 422564 |
| iron transport in mycobacterium smegmatis: uptake of iron from ferriexochelin. | | 1979 | 430028 |
| preparation and some properties of deoxyribonucleic acid polymerase of high specific activity from mycobacterium smegmatis. | | 1979 | 437282 |
| molecular forms of deoxyribonucleic acid present in the purified enzyme from mycobacterium smegmatis [proceedings]. | | 1979 | 437283 |
| interfamilial transfer of amber suppressor gene for the isolation of amber mutants of mycobacteriophage i3. | a suppressor-containing strain of mycobacterium smegmatis sn2 was isolated by transferring an amber suppressor carried on the plasmid of pseudomonas pseudoalcaligenes era through transformation. amber mutants of mycobacteriophage i3 were isolated. | 1979 | 443992 |
| the major mycolic acids of mycobacterium smegmatis. characterization of their homologous series. | | 1979 | 447680 |
| structures of the homologous series of monoalkene mycolic acids from mycobacterium smegmatis. | the positions of localization of the single carbon-carbon double bond in homologs of c series mycolic acids from mycobacterium smegmatis have been established by 1) combined ammonia chemical ionization mass spectrometry/gas-liquid chromatography of aldehyde ozonolysis products, and 2) high resolution electron impact mass spectral analysis of vicinal glycol derivatives as their trimethylsilyl ethers. these studies have revealed that each homolog is a mixture of two isomers differing in double bon ... | 1979 | 447681 |
| purification and subunit structure of propionyl coenzyme a carboxylase of mycobacterium smegmatis. | propionyl-coa carboxylase (ec 6.4.1.3) has been purified from mycobacterium smegmatis. it has a molecular weight of about 500,000. on sodium dodecyl sulfate gels it dissociates into two subunits with molecular weights of 64,000 and 57,000. there are 3.8 mol of biotin/500,000 g of protein. the biotin is associated entirely with the heavier subunit. the enzyme also used acetyl-coa as a substrate. no other acetyl-coa carboxylase could be detected in this organism. | 1979 | 447686 |
| amylooligosaccharides from mycobacterium smegmatis. | in early stationary phase of growth, mycobacterium smegmatis cultures accumulate amylooligosaccharides (alpha 1 leads to 4-glucooligosaccharides) up to the undecasaccharide. although m. smegmatis also makes an acylated polymethylpolysaccharide that is predominantly and alpha 1 leads to 4-glucan, we conclude that these oligosaccharides are precursors of glycogen rather than lopopolysaccharide. | 1979 | 457603 |
| conformational changes associated with complex formation between a mycobacterial polymethylpolysaccharide and palmitic acid. | the anomeric proton magnetic resonances of mycobacterium smegmatis 3-o-methylmannose polysaccharide have chemical shifts intermediate betwen those of nonaglucoamylose and alpha-cyclodextrin, and on addition of palmitic acid most of these resonances are shifted upfield toward that of the cyclodextrin. this suggests that the methylated polysaccharide could have a conformation with some secondary structure intermediate between those of the two reference compounds, and that it forms a tightened coil ... | 1979 | 457682 |
| purification, properties, and cytotoxic effect of a bacteriocin from mycobacterium smegmatis. | the bacteriocin produced by mycobacterium smegmatis atcc 14468 was isolated, and a study was made of its chemical, physical, and biological properties. no appreciable bacteriocin activity was found in the culture supernatant fluids, but it was released in appreciable quantities after disruption of the cells. the material was purified 49-fold by means of chromatography on diethylaminoethyl-cellulose, ammonium sulfate fractionation, gel filtration on sephadex g-200, and chromatography on diethylam ... | 1979 | 464582 |
| mycobacterium smegmatis ferredoxin: a unique distribution of cysteine residues constructing iron--sulfur clusters. | | 1979 | 467663 |
| intralesional immunotherapy of malignant melanoma with mycobacterium smegmatis cell wall skeleton combined with trehalose dimycolate (p3). | the clinical efficacy of intralesional immunotherapy utilizing mycobacterium smegmatis cell wall skeleton (cws) and trehalose dimycolate attached to oil droplets was investigated in 15 patients with advanced malignant melanoma. patients received 300 microgram to 1050 microgram of the cws combined with one-half that amount of trehalose dimycolate every 1 to 2 weeks for a total of 8 treatments. therapy was continued if regression of injected lesions only occurred. therapy was discontinued if regre ... | 1979 | 476567 |
| inhibition by ethambutol of mycolic acid transfer into the cell wall of mycobacterium smegmatis. | ethambutol simultaneously inhibited the transfer (presumably via mycolyl acetyl trehalose) of mycolic acids into the cell wall and stimulated the synthesis of trehalose dimycolates of mycobacterium smegmatis. structural similarities of the drug and mycolyl acetyl trehalose suggested that competitive inhibition was involved. | 1979 | 485133 |
| a compound representing the d-glycerate terminus of the methylglucose-containing polysaccharide of mycobacterium smegmatis. | in order to study the structure of the methylglucose-containing polysaccharide (mgp) of mycobacterium smegmatis by nmr spectroscopy, we have prepared the model compound o-alpha-d-glucopyranosyl-(1 leads to 2)-d-glyceric acid. this compound, which represents the aglycon-containing terminus of mgp, was made from leucorse [o-alpha-d-glucopyranosyl-(1 leads to 5)-d-fructopyranose] by successive treatment with sodium borohydride, lead tetraacetate, and hypobromite. the structure of o-alpha-d-glucopyr ... | 1979 | 497149 |
| structure of the flavin adduct formed in the suicide reaction of alpha-hydroxybutynoate with d-lactate dehydrogenase. | the zn-dependent flavoenzyme d-lactate dehydrogenase from megasphaera elsdenii is irreversibly inactivated by the d form of the suicide substrate 2-hydroxy-3-butynoic acid. the process of inactivation involves formation of a new pink chromophore, which can be released in intact form from the protein and which was purified to homogeneity by affinity chromatography. inactivation involves covalent addition of the suicide substrate to the flavin coenzyme. the optical spectra indicate an elongation o ... | 1979 | 497164 |
| effect of a bacteriocin produced by mycobacterium smegmatis on growth of cultured tumor and normal cells. | growth-inhibitory effects of a partially purified bacteriocin derived from mycobacterium smegmatis atcc 14468 on various animal cells transformed by tumor viruses, human malignant cells, and normal cells in the same species were studied. a growth-inhibitory effect of the bacteriocin on these cultured cells was determined by counting the residual cells. the bacteriocin inhibited virally transformed animal cells (mks-a tu-7, 155-4 t2, and xc cells) and human malignant cells (as-ii and hgc-27 cells ... | 1979 | 498138 |
| effect of growth temperature on the lipid composition of mycobacterium smegmatis atcc 607. | the total lipid content of mycobacterium smegmatis atcc 607 was the same whether it was grown at 27 or 37 degrees c. the total phospholipid content, however, increased significantly at 27 degrees c. phosphatidylethanolamine increased most markedly with a simultaneous decrease in phosphatidylinositol mannosides. among individual phosphatidylinositol mannosides, tri- and tetra-acylated dimannophosphoinositides and tetra-acylated hexamannophosphoinositides all decreased at the lower growth temperat ... | 1979 | 512636 |
| purification and characterization of 3-hydroxyacyl-coa dehydrogenase of mycobacterium smegmatis. | 3-hydroxyacyl-coa dehydrogenase [ec 1.1.1.35] was purified 100-fold to homogeneity from crude extracts of mycobacterium smegmatis, using ammonium sulfate fractionation, gel filtration, and chromatography on deae-cellulose, hydroxyapatite, and nad-sepharose 4b columns. its molecular weight was estimated to be 50,300 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. nadh acted twelve times more efficiently than nadph as an electron donor for the reduction of 3-ketoacyl-coa, and there w ... | 1979 | 521433 |
| differentiation of rapidly growing mycobacteria with trimethoprim (tmp). | inhibition of mycobacterium smegmatis, m. vaccae, and m. diernhoferi by trimethoprim (tmp) in both liquid and solid media is described. other mycobacteria were not inhibited by the same concentrations. the selective inhibition of the above strains, particularly m. smegmatis, by tmp could be used for differentiation of these species from other fast-growing acid-fast bacteria. | 1979 | 540268 |
| [steroid-transforming enzymes from microorganisms. x. enrichment of a 4-en-3-oxosteroid-5 alpha-reductase from mycobacterium smegmatis as well as separation and enrichment of the apoenzyme by means of affinity chromatography]. | the 4-en-3-oxosteroid-5 alpha-reductase from mycobacterium smegmatis was bound biospecifically on the affinant containing an immobilized testosterone ligand. the enzyme obtained by elution with ethylene glycol and urea in a 32 fold purity has a s. a. of 8.73 x 10(-3) microm androstenedione min-1 mg-1. the coenzyme (fad) could be separated from the immobilized enzyme substrate complex on the affinity matrix, in the presence of (nh4)2so4 at ph 3.0. after elution of the apoenzyme 97% of the initial ... | 1979 | 543159 |
| structural and immunochemical characterization of the acidic arabinomannan of mycobacterium smegmatis. | a serologically active, acidic arabinomannan has been isolated from mycobacterium smegmatis. the polysaccharide contains approximately 56 arabinosyl and 11 mannosyl residues, and 2 phosphate, 6 monoesterified succinate, and 4 ether-linked lactate groups. after saponification to remove succinyl groups, the polysaccharide can be separated into phosphorylated (55%) and nonphosphorylated (45%) forms, the former containing a little more arabinose and a little less mannose than the latter. the structu ... | 1979 | 573662 |
| immunochemical and structural integrity of surface protein antigens of mycobacteria during separation from armadillo liver tissue. | surface proteins of mycobacterium smegmatis were iodinated using the lactoperoxidase method. sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated two major surface proteins in the radiolabelled m. smegmatis. both surface proteins were released from m. smegmatis using the nonionic detergent triton x-100. the major surface component was sensitive to pronase digestion and contained no detectable carbohydrate. the second radiolabelled component was found to be of low molecular weig ... | 1979 | 573748 |
| steroid transforming enzymes from microorganisms--iii. properties of 4-ene-3-oxosteroid-5alpha-reductase from mycobacterium smegmatis. | | 1977 | 592797 |
| ultrastructure of nocardia-like variants of mycobacterium smegmatis and chemical composition of the basal cell wall layer. | mycobacterium smegmatis, its orange-red--pigmented (or) variants, and back mutant strains were examined by electron microscopy using ultrathin sectioning, negative or positive staining, and freeze-fracture-etching methods. the parental and back mutant strains showed almost identical ultrastructures. specifically, thick ramified fibers measuring about 15 nm in diameter were always visible in the positively stained cell wall, although they were not readily visualized with negative staining or free ... | 1977 | 597795 |
| glutamate dehydrogenase of mycobacterium smegmatis. | | 1977 | 615109 |
| pentose metabolism in mycobacterium smegmatis: comparison of l-arabinose isomerases induced by l-arabinose and d-galactose. | d-galactose, which did not serve as a growth substrate, was found to induce an l-arabinose isomerase of similar properties to the l-arabinose-induced l-arabinose isomerase. in both cases the ph profiles, ph stability, optimum temperature, heat stability, substrate specificity, metal ion requirements, mobility on polyacrylamide gel electrophoresis, and kinetic properties of the induced isomerases were identical. it appears possible that d-galactose was incorporated into the cells by an l-arabinos ... | 1978 | 618845 |
| turnover of lipids in mycobacterium smegmatis cdc 46 and mycobacterium phlei atcc 354. | the rates of breakdown and renewal of individual lipids in cultures of mycobacterium smegmatis cdc 46 and mycobacterium phlei atcc 354 were investigated by means of a pulse labelling technique using palmitate-1-14c. the results indicated that in growing cultures of both strains phospholipids were broken down, and cardiolipin had a very rapid turnover. in chase experiments, almost 45% and 40% of the radioactivity of this component were lost respectively from m. smegmatis and m phlei during one ge ... | 1978 | 623497 |
| s-trifluoroacetonyl-coenzyme a:a 19f analogue of acetyl-coenzyme a. | s-trifluoroacetonyl-coenzyme a has been synthesized in 87% yield by reaction of 1,1,1-trifluoro-3-bromopropanone with trilithium coenzyme a in presence of pyridine. the compound was characterized by its ultraviolet absorption spectrum and 1h and 19f nuclear magnetic resonance spectra. the alpha-methylene protons of the s-trifluoroacetonyl group exchanged with d2o and showed a pka of 9.85 in s-trifluoroacetonylmercaptoethanol. s-trifluoroacetonyl-coenzyme a is a competitive inhibitor of porcine h ... | 1978 | 629939 |
| subunit structure of mycobacterium smegmatis fatty acid synthetase. evidence for identical multifunctional polypeptide chains. | fatty acid synthetase from mycobacterium smegmatis has been purified to near homogeneity as judged by a variety of electrophoretic criteria under both native and dissociating conditions. a single protein band was obtained on gel electrophoresis in sodium dodecyl sulfate or 8 m urea at various ph values and on isoelectric focusing in 8 m urea. a subunit molecular weight of about 290,000 was found by polyacrylamide gel electrophoresis in sodium dodecyl sulfate or by sedimentation equilibrium ultra ... | 1978 | 632292 |
| studies on the trehalose-phosphate synthase of mycobacterium smegmatis: binding of heparin to the enzyme. | | 1978 | 637569 |
| physiological factors involved in the transformation of mycobacterium smegmatis. | transfer of streptomycin resistance and changes from methionine and leucine auxotrophy to prototrophy were achieved in mycobacterium smegmatis by transformation. recipient cells were more resistant to mitomycin c and methyl methlanesulfonate treatments than were wild-type cells. a high level of calcium ions was essential for transformation, especially during dna adsorption, whereas the presence of magnesium ions and the exposure of recipient cells to mild doses of uv light enhanced recombination ... | 1978 | 641008 |
| amounts of iron, haem and related compounds in mycobacterium smegmatis grown in various concentrations of iron [proceedings]. | | 1978 | 648724 |
| the transport of iron from ferriexochelin by mycobacterium smegmatis [proceedings]. | | 1978 | 648725 |
| antitubercular 2,8-bis(alkylaminomethyl)phenazines. | the preparation and antitubercular properties of a series of 2,8-bis(alkylaminomethyl)phenazines are described. these compounds all inhibited the growth of mycobacterium smegmatis atcc 607 in vitro. 2,8-bis(dibutylaminomethyl)phenazine (5c) was also active against a lethal mycobacterium tuberculosis h37rv infection in mice. | 1978 | 650670 |
| antimicrobial activities of constituents of uvaria chamae. | the antimicrobial activities of a number of cytotoxic c-benzylated flavonoids from uvaria chamae have been determined. the minimum inhibitory concentration values of these flavonoids and certain of their derivatives against straphylococcus aureus, bacillus subtilis, and mycobacterium smegmatis compare favorably with those of streptomycin sulfate. | 1978 | 651562 |
| dd-carboxypeptidase activity of membrane fragments of mycobacterium smegmatis. enzymatic properties and sensitivity to beta-lactam antibiotics. | a dd-carboxypeptidase activity is present in membrane fragments of mycobacterium smegmatis. kinetic parameters of the enzymatic activity have been studied using udp-n-glycolylmuramyl-l-alanyl-gamma-d-glutamyl-meso-2,2'-diaminopimelyl-d-[14c]alanyl-d-[14c]alanine as substrate. the dd-carboxypeptidase can be solubilized by triton x-100 and genapol x-100. it is inhibited by beta-lactam antibiotics although intact cells of m. smegmatis are insensitive to that kind of antibiotics. inhibition by penic ... | 1978 | 658048 |
| in vitro cytotoxicity of peritoneal macrophages activated with mycobacterium smegmatis. | incorporation of 3h-tdr into el4 leukemic cells in vitro was inhibited by peritoneal exudate cells (pec) harvested from syngeneic c57bl/6j mice given an intraperitoneal (i.p.) injection of 1x10(7) viable mycobacterium smegmatis atcc 607 (smeg) 4 days before. this phenomenon was also observed in the following five systems of pec from animals and syngeneic tumor cells: c57bl/6j mice and b16 melanoma; dba/2 mice and p815 mastocytoma; swm/ms mice and k5 fibrosarcoma; balb/c, nu/nu mice and kkn-1 fib ... | 1978 | 661626 |
| isolation and structural determination of a "cord-factor" (trehalose 6,6' dimycolate) from mycobacterium smegmatis. | | 1978 | 668030 |
| alkaloids of thalictrum xxvi. new hypotensive and other alkaloids from thalictrum minus race b. | the roots of t. minus race b have yielded 9 alkaloids, thalirabine (1), thaliracebine (13), thalfine (19), thalfinine (20), thalrugosaminine (22), thalidasine (13), obaberine (24), thaliglucinone (25) and (s)-reticuline (26). the first two, possessing marked hypotensive activity, were assigned complete structures by physical and chemical methods. thalfine (19) was given s-configuration at its one asymmetric center and was converted to thalfinine (20) and epithalfinine (21), thus the stereochemis ... | 1978 | 672463 |
| alkaloids of thalictrum xxvii. new hypotensive aporphine-benzylisoquinoline derived dimeric alkaloids from thalictrum minus race b. | the roots of t. minus race b have yielded, in addition to adiantifoline (1) previously isolated from this source, two new related alkaloids, thaliadine (2) and thaliadanine (5). both were assigned complete structures by spectral methods and by chemical interconversion to adiantifoline or its product. o-desmethyladiantifoline should have structure 14, rather than the previously reported 5. all three isolated alkaloids show hypotensive activity in rabbits, and thaliadanine (5) has antimicrobial ac ... | 1978 | 672464 |
| inhibition of growth and cell wall synthesis of mycobacterium smegmatis by d-threonine. | | 1978 | 672670 |
| alkaline phosphatase activity in mycobacterium smegmatis. | | 1978 | 680866 |
| characterisation of mycobacteriophage atcc 11759. unusual physiocochemical properties of its dna. | growth conditions and a purification procedure for mycobacteriophage atcc 11759, a lytic phage for mycobacterium smegmatis, are described. the phage is a large dna phage with a very long tail (240 nm). a study of its dna revealed three interesting features. 1. after denaturation the dna molecule yields two strands of different buoyant densities. 2. the native dna has unusual physical properties: its buoyant density in csc1 is very low (1.654 g/cm3), its sedimentation rate is lower than expected ... | 1978 | 720334 |
| ammonia assimilation by mycobacterium smegmatis during growth on different nitrogenous sources. | | 1978 | 721126 |
| adenylate cyclase activity of mycobacterium smegmatis cdc 46. | | 1978 | 721127 |
| enzymes of dicarboxylic acid metabolism in mycobacterium smegmatis cdc 46. | | 1978 | 738746 |
| the separation of the mycobactins from mycobacterium smegmatis by using high-pressure liquid chromatography. | the family of mycobactins from mycobacterium smegmatis were resolved into seven fractions by high-pressure liquid chromatography. this separation was by virtue of the differences in length and character of the long acyl substituents as shown by g.l.c. of the methyl esters of the isolated fatty acids from the fractions. as t.l.c. could also resolve the individual mycobactin fractions, it too must rely on the same differences to effect separation. as the lengths of the acyl chains were modulated b ... | 1978 | 743238 |
| physicochemical and biological properties of mycobacteriocin m12 produced by mycobacterium smegmatis atcc 25855. | a mycobacteriocin (m12) produced by mycobacterium smegmatic atcc 25855 was partially purified by ammonium sulphate precipitation followed by deae-cellulose chromatography and sephadex g100 chromatography. production of m12 was maximal when bacteria were harvested after 3 d cultivation in liquid medium and disrupted by sonication. the molecular weight of m12, estimated by sephadex g100 chromatograpy, was about 85000. m12 was sensitive to proteolytic enzymes but resistant to dnaase and rnaase, and ... | 1978 | 745002 |
| uptake & transport of hexoses in mycobacterium smegmatis. | | 1978 | 751909 |
| nonspecific resistance to infection induced in mice by a water-soluble adjuvant derived from mycobacterium smegmatis. | the effect of a nontoxic, water-soluble adjuvant (neo-wsa) from delipidated cells of mycobacterium smegmatis on the susceptibility of mice to infection with four challenge organisms was studied. an intravenous dose of 1 mg of neo-wsa per mouse 24 hr before challenge enhanced resistance to infection with a fungus (candida albicans), a gram-negative bacterium (klebsiella pneumoniae), and a gram-positive bacterium (streptococcus pneumoniae). protection by neo-wsa was not significant when the mice w ... | 1976 | 772130 |
| concentration and purification of mycobacteriophages with polyethylene glycol 6000. | experiments were performed to concentrate and purify mycobacteriophages with polyethylene glycol 6000; 6, 9, and 8 per cent polyethylene glycol 6,000 proved to be optimal concentrations for precipitating phages of mycobacterium phlei, mycobacterium smegmatis strain butyricum, and mycobacterium smegmatis strain rabinowitz, respectively. preparative polyethylene glycol reverse gradients were constructed for further concentration and purification of the precipitated phages. with the method describe ... | 1976 | 779553 |
| presence of two forms of palmityl-coa-acp-transacylase in homogenates of mycobacterium smegmatis, purification using the monomer-polymer interconvertibility. | homogenates of mycobacterium smegmatis have been shown to contain, among other acyl-transfer enzymes, two entities endowed with palmityl-coa-acp-transacylase activity (1973, this journal, 55, 1381-1394). the present report demonstrates that these two entities, now called palmityl-coa-acp-transacylase i and palmityl-coa-acp-transacylase ii, following their elution rate from a sephadex g-150 column, are two interconvertible forms of the same enzyme. form ii, apparently predominant in the crude hom ... | 1976 | 782565 |
| nonspecific macrophage activation by systemic adjuvants. evaluation by lysosomal enzyme and in vitro tumoricidal activities. | six systemic adjuvants: living bacillus calmette-guérin (bcg), hydrosoluble extracts from bcg and from mycobacterium smegmatis, bacterial lipopolysaccharide, lentinan and levamisole, have been tested for their ability to induce macrophage activation in mice. the first four adjuvants mentioned increase phosphatase activity of peritoneal macrophages and make them nonspecifically cytotoxic for tumor cells in vitro. the intensity of these phenomena vary with route and time of administration. in cont ... | 1976 | 786904 |
| the effect of leukocyte hydrolases on bacteria. iii. bacteriolysis induced by extracts of different leukocyte populations and the inhibition of lysis by macromolecular substances. | the lysis of 14c-labeled bacteria by hydrolases of human and rabbit leukocytes was studied in vitro. while staphylococcus albus, streptococcus faecalis, and streptococcus mutans were highly susceptible to lysis, staphylococcus auresus was intermediate in its susecptibility to lysis by the leukocyte enzymes. group a streptococcus, listeria monocytogenes, shigella flexneri, escherichia coli, and mycobacterium smegmatis were very resistant to degradation by these enzymes. the lytic activity of leuk ... | 1975 | 804017 |