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hairy cell leukemia: differences in phagocytic capacity of cells in vitro.hairy cells from eight patients with hairy cell leukemia were evaluated with both light and transmission electron microscopy for their capacity to phagocytose zymosan, latex, staphylococcus aureus, and pseudomonas aeruginosa. in two patients, there was no phagocytosis of any of these substances; cells from three patients phagocytosed only latex; two, all except pseudomonas; and one, all 4 substances. hairy cells became relatively smooth while in culture with staphylococcus, but no surface change ...197937638
heat inactivation of mycobacterium avium-mycobacterium intracellulare complex organisms in aqueous suspension.isolants from swine and from humans representing serotypes 1, 2, 4, 8, and 10 of the mycobacterium avium-mycobacterium intracellulare complex were compared for heat tolerance in aqueous suspension. the most heat-resistant isolant found was a serovar 10 isolated from a human. this isolant was examined further to determine the rate of kill at various temperatures and ph's, the effect of meat protein and fat, and the effect of nitrite. kill rates were not significant at 60 degrees c or below. decim ...197944444
in vitro susceptibility of mycobacteria, especially of mycobacterium intracellulare, to 5-fluorouracil and pattern of development of resistance of mycobacterium tuberculosis to the drug. 1979116112
bronchocentric granulomatosis: a complication of allergic bronchopulmonary aspergillosis.hypersensitivity to the fungal antigens of aspergillus fumigatus may result in a spectrum of immune injury collectively known as allergic bronchopulmonary aspergillosis (abpa). this report describes a 14-yr-old boy who presented clinical findings consistent with abpa,including a history of asthma, blood eosinophilia, serum precipitins, and ige antibodies to aspergillus fumigatus. sputum aspergillus, pulmonary infiltrates, and dual types i and iii skin reactions to aspergillus fumigatus were obse ...1977319138
an epizootic of mycobacterium intracellulare, serotype 8 infection in swine.eight strains of mycobacteria isolated from tuberculous lymph nodes of swine were identified as mycobacterium intracellulare, serotype 8. the infected swine were borne in a large breeding herd and as 8 weeks old piglets distributed to a number of farms for fattening. autopsy material from the breeding herd, incriminated as the primary source of m. intracellulare, serotype 8 infection, has not been available for bacteriological examination, but none of a number of sows slaughtered showed any visi ...1977325503
the pleiotropic effect of spontaneous single-step variant production in mycobacterium intracellulare.a strain of m. intracellulare, at 13786, derived from human sputum, gave rise to transparent and opaque colony forms which were cloned and investigated. from the results the conclusion was drawn that the opaque form was a mutant of the transparent type and possessed alterations in the cell envelope which were responsible for enhanced permeability. the opaque form was more susceptible to a number of antibiotics; it grew faster in standard medium, and was not dependent on tween for dextrose utiliz ...1978356253
pulmonary mycobacterial infections due to mycobacterium intracellulare-avium complex. clinical features and course in 100 consecutive cases.one hundred consecutive cases of pulmonary infection due to mycobacterium intracellulare-avium seen during a 3 1/2-year period qualified for review on the basis of a compatible chest x-ray film, repeated isolations from cultures of sputum, and follow-up of three to eight years. infections with m intracellulare-avium represented 27 percent of all mycobacterial infections seen during this period, including those due to m tuberculosis. the cases of disease due to m intracellulare-avium were predomi ...1979421545
structural studies on the type-specific antigens and lipids of the mycobacterium avium. mycobacterium intracellulare. mycobacterium scrofulaceum serocomplex. mycobacterium intracellulare serotype 9. 1979438184
long-term results of medical treatment in mycobacterium intracellulare infection. 1979474589
elimination of mycobacterium intracellulare from sputum after bronchial hygiene.three cases are presented, each of which had more than 20 positive cultures of sputum for mycobacterium intracellulare, infiltrations, and a history of bronchiectasis. two had complete and the third nearly complete, sputum conversion during a program of bronchial hygiene without chemotherapy. it is probable that these cases were only noninvasive colonizations in previously diseases lungs. recommendations are given for a therapeutic approach in such cases.1979477441
mycobacterium intracellulare infection in a water monitor.mycobacteriosis caused by mycobacterium intracellulare serotypes davis (8) and altman (18) is described in a water monitor (varanus semiremex). infection with this of organism has not been reported previously in reptiles in australia.1979480513
[a study on experimental mycobacterioses provoked by atypical mycobacteria. 2. combined antituberculous chemotherapy against conventional mice infected intravenously with mycobacterium intracellulare (author's transl)]. 1979502152
esophageal compression in association with silicosis and mycobacterium intracellulare.an enameler with dysphagia was found to have extrinsic compression of the esophagus by enlarged mediastinal lymph nodes. scalene lymph node biopsy revealed silicosis, and tissue cultures grew mycobacterium intracellulare. we believe our patient is the first reported to have dysphagia due to silicotic adenopathy complicated by an atypical mycobacteriosis.1979510011
postulated sources of mycobacterium intracellulare and mycobacterium scrofulaceum infection: isolation of mycobacteria from estuaries and ocean waters.mycobacteria biochemically and serologically similar to those isolated from humans have been found in estuaries and ocean waters in the south-eastern united states. this is consistent with the hypothesis that aerosols from these waters are one potential source of infection with these mycobacteria.1979517862
[sensitivities of atypical mycobacteria to various drugs. v. sensitivities of mycobacterium intracellulare to some aminoglycoside and peptide antibiotics (author's transl)]. 1979537304
activity of some antileprosy compounds against mycobacterium intracellulare in vitro.a series of 12 compounds, consisting of 3 known antileprosy drugs (dapsone, b663, and ciba 1906) and 9 structural derivatives of dapsone, were tested for their antimycobacterial activity against 50 strains of mycobacterium intracellulare. detailed investigations of b663, the only compound showing considerable in vitro activity, were carried out. all of the strains of m. intracellulare were inhibited by a b663 concentration of 1.6 mug per ml, and 88% were inhibited by a drug concentration of 0.8 ...1977557943
[the behaviour of mycobacterium intracellulare serotyp davis and mycobacterium avium in the head region of sphagnum moss vegetation after experimental inoculation (author's transl)].after the inoculation in the head region of sphagnum moss vegetation (sph. magellancium-erica association and sph. apiculatum-eriophorum angustifolium ass.) reach the mycobacteria the hyaline cells of sphagnum (fig. 1). the count of m. intracellulare serotyp davis decreases rapidly already in three days after the inoculation of sph. magellanicum ass. and this trend in the course of incubation is being continued. in 27 days following the inoculation only 2 of 7 specimens contained a small amount ...1978566009
antimycobacterial activity of some potential chemotherapeutic compounds.fourteen compounds were tested in vitro for activity against mycobacterium intracellulare and other pathogenic mycobacteria. only clofazimine and chaulmoogric acid showed significant activity against m. intracellulare. in view of known minimal side effects of clofazimine further studies are warranted for this drug in chemotherapy of m. intracellulare infections.1978567395
heat inactivation of mycobacterium avium-mycobacterium intracellulare complex organisms in meat products.wieners and sausages were prepared which contained the most heat-tolerant representative of the mycobacterium avium-mycobacterium intracellulare complex we were able to obtain. they also were prepared with infected tissues obtained from tuberculous swine. processing conditions were as varied as possible. neither incorporation of sodium nitrite in the emulsion nor presence of smoke during processing altered the heat susceptibility of the organisms. substantial killing of the organisms occurred as ...1979575610
disseminated histiocytosis and intestinal malakoplakia. occurrence due to mycobacterium intracellulare infection.disseminated histiocytic proliferation and intestinal malakoplakia due to mycobacterium intracellulare occurred in a 2-year-old white boy. light and electron microscopic examination of autopsy material disclosed disseminated histiocytosis and malakoplakia of the intestine with numerous intracellular acid-fast bacilli. the isolated organism produced a similar disseminated histiocytosis and finally death when injected into experimental animals. a review of the literature showed only 12 previously ...1979582266
serological investigation of strains of mycobacterium avium and mycobacterium intracellulare isolated from animal and non-animal sources. 1978662590
mycobacterium intracellulare presenting as a sarcoid-like illness.a combined infection with mycobacterium intracellulare and histoplasmosis in a patient with end-stage sarcoidosis is reviewed. a healthy skepticism should be applied to all but the most classic cases of sarcoidosis. prolonged follow-up with repeated attempts to demonstrate a specific causative agent will decrease the possibility of overlooking treatable infections. an initial work-up of a patient with sarcoidosis includes fungal immunologic tests, which if positive can lead to further investigat ...1978663741
the relative drug susceptibility of opaque colonial forms of mycobacterium intracellulare-avium: does it affect therapeutic results? 1978666109
[case of mycobacteriosis caused by mycobacterium intracellulare in a patient with pneumoconiosis]. 1978693334
inhibition of mycobacterium intracellulare by some vitamin k and coenzyme q analogues.because vitamin k is present in several species of mycobacteria, and because coenzyme q has been found to stimulate the growth of mycobacterium tuberculosis and atypical mycobacteria, 8 quinones that can be considered to be dual analogues of vitamin k and coenzyme q were tested for their activity against mycobacterium intracellulare. one compound, 6-cyclo-octylamino-5,8-quinolinequinone (cqq) exhibited considerable activity in vitro against several strains of mycobacterium intracellulare at a co ...1978707875
susceptibility of newborn and adult guinea pigs to mycobacterium intracellulare and m. kansasii.six groups of guinea pigs, each consisting of 30 3-month-old and 20 7-day-old animals, received intraperitoneal inoculations with one of four strains of mycobacterium intracellulare or one of two strains of m. kansasii. three similar control groups received either an intraperitoneal injection of m. tuberculosis, no injection, or a sterile injection. guinea pigs were sacrificed at 3, 5, 10, 20, 30, and 90 days. lungs, spleen, liver and peritoneal fluid were cultured. lungs were also examined hist ...1975809838
[virulence of mycobacterium avium and mycobacterium intracellulare in pigs during experimental and spontaneous infection].the virulence of m. avium and m. intracellulare was studied in 16 experimentally infected pigs and on 174 animals coming from herds spontaneously infected with the mentioned mycobacterium species. the result of the patho-anatomic examination and cultivation from samples of lymph nodes, organs, muscles, and excrements proved a higher virulence of m. avium to experimentally and spontaneously infected pigs, as compared with m. intracellulare. the strains of m. avium and m. intracellulare sensibiliz ...1976828995
pathological changes in calves injected intradermally with mycobacterium intracellulare serotype davis and m. avium serotype 2.three strains of the m. intracellulare-m. avium complex were injected intradermally into 12 calves. m. intracellulare serotype davis (m. avium complex serotype 8) was injected into five calves. one calf died with disseminated caseous granulomas at 79 days; three calves killed between 56 and 82 days after inoculation had widely disseminated caseous or caseous or caseocalcareous granulomas. one calf killed at 173 days had only encapsulated granulomas at the injection site and its regional lymph no ...1977850995
simultaneous infection of the central nervous system with cryptococcus neoformans and mycobacterium intracellulare.a 46-year-old woman with cryptococcal meningoencephalitis also had mycobacterium intracellulare isolated from her cerebrospinal fluid. she was treated with amphotericin b,5-fluorocytosine, and antituberculous agents (isoniazid and rifampin). she gradually improved and was well, except for residual neurologic damage, three years after discharge from the hospital. no evidence of significant underlying disease has been found.1977877651
permeability barrier to rifampin in mycobacteria.a strain of mycobacterium intracellulare that exhibited natural resistance to rifampin was isolated. the ribonucleic acid polymerase was found to be susceptible to rifampin. studies with tween 80 suggested that a permeability barrier against rifampin was present in the intact organism. a type strain of mycobacterium smegmatis, atcc 607, considered resistant to rifampin also demonstrated a permeability barrier. the possibility that rifampin resistance in naturally occurring strains of mycobacteri ...1977879732
[transfer factor therapy in a patient with anergic pulmonary tuberculosis].report on a patient suffering from severe, relapsing pulmonary tuberculosis showing progressive clinical deterioration accompanied by the appearance of cutaneous anergy to tuberculin. in addition, the sputum cultures showed growth of mycobacterium intracellulare. during therapy with transferfactorzurich there was a slow but impressive clinical improvement, the skin reactivity to tuberculin was reconstitued and the sputum cultures became negative. the radiological findings remained unchanged.1977929133
[studies on 'marks' strains which show several unusual characteristics among mycobacterium intracellulare. some observations on the relationship between m. intracellulare and m. scrofulaceum (author's transl)]. 1976966408
mycobacterium intracellulare from public bath water. 19761018346
peripheral nervous system involvement in experimental tuberculosis.following intravenous injection of mycobacteria in the mouse the bacilli are regularly found in the peripheral nervous system after the 5th day. the peripheral nerves and autonomic ganglia are involved at the level of the perineural cells and the sheath of schwann. when the organisms are of low virulence (bcg, mycobacterium intracellulare) the development of the nodules is limited. in the case of a virulent strain (ravenel) extensive granulomas are found but epitheloid cells, always found in oth ...19751101166
[roentgenographic features of lung disease due to mycobacterium intracellulare (primary and secondary infection)]. 19751127844
serotype of mycobacterium avium-mycobacterium intracellulare complex that caused lung disease in japan. 19751160203
postulated source of mycobacterium intracellulare (battey) infection. 19751180254
[experimental studies on allergenic effect of mycobacterium intracellulare in cattle].mycobacterium intracellulare, isolated from sawdust used as litter, was inoculated into 25 bullocks. six developed a doubtful reaction to mammalian tuberculin and the remainder stayed negative. with avian tuberculin, 8 became positive, 10 doubtful and 7 remained negative. using the interpretation key described by goetze, lauterbach and nassal, the reactions were shown to be para-allergic. at slaughter there was no evidence of tuberculous lesions.19751200752
mycobacterium intracellulare infection in pigs. 19761260638
[infection of the lung due to mycobacterium intracellulare improving in a short period (author's transl)]. 19761271604
[relationship between occurrence of negative conversion and tuberculin reactivity in lung disease due to mycobacterium intracellulare (author's transl)]. 19761271607
the catalase-peroxidase of mycobacterium intracellulare: nucleotide sequence analysis and expression in escherichia coli.the activation of catalase genes in response to oxidative stress may contribute to the intracellular survival of mycobacteria. in this report, the nucleotide sequence of a mycobacterial catalase gene is described. the deduced protein sequence of this mycobacterium intracellulare gene (mi85) was 60% identical to the escherichia coli hydroperoxidase i (hpi) protein, 59% identical to the salmonella typhimurium (hpi) catalase, and 47% identical to a bacillus stearothermophilus peroxidase. the mi85 p ...19921336034
use of restriction fragment length polymorphisms resolved by pulsed-field gel electrophoresis for subspecies identification of mycobacteria in the mycobacterium avium complex and for isolation of dna probes.mycobacterial strains from the mycobacterium avium complex were compared with each other and with mycobacterium phlei isolates by restriction endonuclease digestion of chromosomal dna with sspi and analysis by pulsed-field gel electrophoresis. characteristic profiles were observed for known typed strains, and five groups were identified. primary bovine isolates identified as mycobacterium paratuberculosis by classical methods were shown to fall into both the m. paratuberculosis- and m. avium-lik ...19921352787
identification of a beta 1 integrin on mycobacterium avium-mycobacterium intracellulare.mycobacterium avium-mycobacterium intracellulare (mai) is an opportunistic intracellular pathogen responsible for the highest incidence of disseminated bacterial infection in patients with aids. treatment of the infection is extremely difficult and has shown limited efficacy. a critical event in the initiation of a variety of bacterial infections involves the adherence of bacteria to host cell surfaces. in the present study, we have shown that mai organisms bind avidly to extracellular matrix pr ...19921379987
clarithromycin-ciprofloxacin-amikacin for therapy of mycobacterium avium-mycobacterium intracellulare bacteremia in patients with aids.a combination of clarithromycin, ciprofloxacin, and amikacin for the treatment of mycobacterium avium-mycobacterium intracellulare bacteremia was evaluated in 12 aids patients. mycobacteremia cleared in all patients by 2 to 8 weeks of treatment, and symptoms resolved. four patients died; all had negative blood cultures until death, and disseminated m. avium-m. intracellulare complex infection was not considered the primary cause of death.19921387303
[studies on whole chromosomal dna probes from mycobacterium tuberculosis and mycobacterium intracellulare].the whole chromosomal dna probes prepared from m. tuberculosis and m. intracellulare were used to hybridize in dna dot blot and bacterial dot blot hybridization with 9 reference strains of mycobacteria, including m. tuberculosis, m. bovis, m. avium, m. intracellulare, m kansasii, etc. these two probes showed good sensitivity and specificity for m. tuberculosis complex and m. avium complex respectively. using the colony hybridization in situ, we can detect and identify m. tuberculosis with the pr ...19921394583
activity of defensins from human neutrophilic granulocytes against mycobacterium avium-mycobacterium intracellulare.we have examined the activity of defensins from human neutrophilic granulocytes against mycobacterium avium-mycobacterium intracellulare. m. avium-m. intracellulare at 2.5 x 10(6)/ml or 2.5 x 10(8)/ml was cultured in the presence of defensins at 37 degrees c from 4 to 48 h. after incubation, cfu were enumerated. human neutrophil peptide 1 (hnp-1) at 5 micrograms/ml had the ability to kill m. avium-m. intracellulare. treatment with hnp-1 resulted in significant (96.3 to 97.7%) killing of m. avium ...19921398982
identification of mycobacterium avium complex strains and some similar species by high-performance liquid chromatography.strains of mycobacterium avium, mycobacterium intracellulare, mycobacterium scrofulaceum, mycobacterium xenopi, and mycobacterium gordonae were identified by high-performance liquid chromatography (hplc) analysis of mycolic acids as bromophenacyl esters. hplc criteria were used to develop a flow chart identification scheme, which was evaluated in our laboratory with a set of 234 strains representing five species and a hitherto undescribed species. correct identifications of m. gordonae and m. xe ...19921400970
bacteriostatic and bactericidal activity of antituberculosis drugs against mycobacterium tuberculosis, mycobacterium avium-mycobacterium intracellulare complex and mycobacterium kansasii in different growth phases.bacteriostatic and bactericidal activities of rifampicin, isoniazid, streptomycin, enviomycin and ethambutol against mycobacterium tuberculosis, mycobacterium avium--m. intracellulare complex and mycobacterium kansasii were studied in different growth phases. bacteriostatic activities of the drugs were similar in different growth phases, except isoniazid. m. tuberculosis was much less susceptible to isoniazid in the lag phase than in the log and the stationary phases. in contrast, bactericidal a ...19921406364
a new glycolipid from mycobacterium avium--mycobacterium intracellulare complex.from a nonpolar lipid fraction of mycobacterium avium--mycobacterium intracellulare complex cell mass, a new glycolipid was obtained, which was shown to be 5-mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl- (1-->1')-glycerol. when examined by tlc, all the 12 strains of this species tested, including clinical isolates, were found to contain this glycolipid. but the glycolipid was not detected in mycobacterium bovis bcg or mycobacterium tubrculosis h37rv.19921420348
skin testing with recombinant mycobacterium intracellulare antigens.the immunoreactivity of four recombinant mycobacterium intracellulare beta-galactosidase fusion proteins, which correspond to 22, 40, 43 and 85 kda m. intracellulare antigens, was assessed. lymphoproliferative assays demonstrated that escherichia coli lysates containing each of the fusion proteins stimulated t cells in vitro. purified preparations of three of these recombinant m. intracellulare antigens (22, 43 and 85 kda) also induced delayed-type hypersensitivity (dth) reactions in sensitized ...19921421343
nucleotide sequence analysis and serologic characterization of the mycobacterium intracellulare homologue of the mycobacterium tuberculosis 19 kda antigen.disseminated mycobacterium avium/mycobacterium intracellulare complex (mac) disease is a frequent complication in patients with the acquired immune deficiency syndrome (aids). in this report, we present the nucleotide sequence of the m. intracellulare mi22 gene. computer sequence comparisons reveal that the mi22 gene, which encodes a serologically active protein, has 78% dna sequence identity and 77% protein sequence identity with the seroreactive 19 kda mycobacterium tuberculosis lipoprotein an ...19921445568
differential susceptibilities of mycobacterium avium and mycobacterium intracellulare to sodium nitrite.sixty-seven of 72 strains of mycobacterium avium (93.1%) were resistant to sodium nitrite at a concentration of 3 mg/ml in 7h11 agar medium, while 57 of 59 strains of mycobacterium intracellulare (96.6%) were susceptible to the agent. the difference in the susceptibilities of m. avium and m. intracellulare to sodium nitrite is therefore useful for the differentiation of the two species.19921452672
mycobacterium intracellulare soft tissue infection. 19921479085
[in vitro susceptibilities of mycobacterium avium and mycobacterium intracellulare to new macrolides, new quinolones, and antituberculous drugs on dubos agar medium].mycobacterium avium and m. intracellulare were isolated from the sputum of patients infected with atypical mycobacteria using 1% ogawa medium and identified by the dna probe test. then the mics of various kinds of drugs against these mycobacterial species were determined on dubos agar medium, and the drug susceptibilities were also determined on 1% ogawa medium in parallel. the drugs tested were new macrolides, such as clarithromycin (cam) and roxithromycin (rxm), new quinolones, such as ofloxac ...19921487866
comparison of the abilities of mycobacterium avium and mycobacterium intracellulare to infect and multiply in cultured human macrophages from normal and human immunodeficiency virus-infected subjects.patients with aids commonly develop disseminated infections with mycobacterium avium (ma) but not its close relative, m. intracellulare (mi). in non-aids patients who have these infections, the two species are about equally distributed. the higher incidence of infection with ma than with mi in aids patients might be due to the selective susceptibility of these patients to ma. this possibility was tested by comparing the abilities of ma and mi to infect and replicate in cultured macrophages from ...19921500179
differentiation between mycobacterium avium and mycobacterium intracellulare by thin-layer chromatography of lipid fraction after incubation with [35s]methionine.mycobacterium avium and mycobacterium intracellulare are differentiated from each other by thin-layer chromatography of lipid fraction extracted after incubation with [35s]methionine. the former contained a petroleum ether-soluble sulfolipid and the latter did not.19921513266
mycobacterium avium and mycobacterium intracellulare chromatotypes defined by curvilinear gradient hplc of mycolic acids.seventy-nine strains of mycobacterium avium complex bacteria (mac), previously characterized by genetic probe analysis, were assayed using two methods of reverse phase high-performance liquid chromatography (hplc) that employed curvilinear gradients. although different in column length and cycle time, the methods produced equivalent results, yielding seven distinct chromatographic patterns (chromatotypes) of m. avium and m. intracellulare based on the ratio of mycolate concentrations in the late ...19921516806
use of dna probes to detect mycobacterium intracellulare and "x" mycobacteria among clinical isolates of mycobacterium avium complex.a mycobacterial dna probe (designated x) was recently developed to help identify mycobacterium avium complex (mac) isolates that are nonreactive with probes specific for m. avium or mycobacterium intracellulare. the prevalence of x probe-positive mycobacteria in clinical specimens and their role in causing disease is unknown. using a dna probe kit that includes the x probe, we characterized 100 consecutive clinical mac isolates as m. avium, m. intracellulare, or x. lysates from 81 of the isolate ...19921607695
mycobacterial diseases other than tuberculosis.the incidence of tuberculosis in the united states declined steadily until 1985, while at the same time, for at least the past 15 years, the frequency of disease attributable to other mycobacteria increased both in actual numbers and in the proportion of the total burden of mycobacterioses. chronic pulmonary disease, lymphadenitis in children, skin and soft-tissue involvement, and infections of the skeletal system were predominant, and the principal etiologic agents were mycobacterium avium/myco ...19921617048
mycobacterium xenopi, mycobacterium fortuitum, mycobacterium kansasii, and other nontuberculous mycobacteria in an area of endemicity for aids.between 1981 and 1990, cultures of specimens from 86 patients at state university of new york-health sciences center at brooklyn were positive for nontuberculous mycobacteria other than mycobacterium avium/mycobacterium intracellulare complex or mycobacterium gordonae. the most common species isolated were mycobacterium xenopi (33), mycobacterium fortuitum (28), mycobacterium kansasii (7), and mycobacterium chelonae (6). thirty-five patients (41%) had clinical and/or serological evidence of huma ...19921617056
comparison of prognosis of pulmonary diseases caused by mycobacterium avium and by mycobacterium intracellulare.a total of 55 patients with pulmonary disease caused by mycobacterium avium and by mycobacterium intracellulare were compared for their prognosis. causative mycobacteria were identified by the dna probes (gen-probe) method. of the 55 patients, 28 had pulmonary disease caused by m avium and the remaining 27, by m intracellulare. of the former group, four patients had progressive disease, and three of them died during the observation period. in contrast, only one of the latter group had progressiv ...19921623801
[antimycobacterial activities of a new quinolone, sparfloxacin].sparfloxacin (spfx), a new quinolone, was studied its in vitro and in vivo activities against various mycobacteria, especially mycobacterium intracellulare. spfx exhibited a potent in vitro activity against m. tuberculosis, m.kansasii and m.fortuitum with mic90 values of 0.2, 6.25 and 1.6 micrograms/ml, respectively, and the potency of spfx was higher than that of ofloxacin (oflx). m.marinum, m.scrofulaceum, m.avium, m.intracellulare, m.chelonae (subsp. abscessus and chelonae) were resistant to ...19911660089
mycobacterium avium-mycobacterium intracellular complex-induced suppression of t-cell proliferation in vitro by regulation of monocyte accessory cell activity.heat-killed whole mycobacterium avium-mycobacterium intracellulare complex (mac) and its lipid component impaired the capacity of human peripheral blood mononuclear cells to proliferate in vitro in response to concanavalin a (cona), purified protein derivative of tuberculin (ppd), and to a lesser degree, phytohemagglutinin stimulation. inhibition by mac was not contingent upon prior exposure of the donor to mac or other mycobacteria and occurred with lymphocytes from tuberculin-negative as well ...19901691144
evaluation of acridinium-ester-labeled dna probes for identification of mycobacterium tuberculosis and mycobacterium avium-mycobacterium intracellulare complex in culture.the detectability of mycobacteria in culture by the use of nonisotopic, chemiluminescent dna probes for mycobacterium tuberculosis and the m. avium-m. intracellulare complex (mac) was evaluated and compared with that by the use of 125i-labeled dna probes for the same mycobacteria. in the assay, rrna-directed dna probes labeled with acridinium ester (ae-dna probes) were used. unhybridized probes were chemically degraded, and the esterified acridinium on the hybridized probes was hydrolyzed by the ...19911723071
epidemiology of infection by nontuberculous mycobacteria. mycobacterium avium, mycobacterium intracellulare, and mycobacterium scrofulaceum in acid, brown-water swamps of the southeastern united states and their association with environmental variables.mycobacterium avium, mycobacterium intracellulare, and mycobacterium scrofulaceum (mais) organisms were isolated and identified from waters, soils, aerosols, and droplets ejected from water collected from four geographically separate aquatic environments (okefenokee swamp, ga; dismal swamp, va; claytor lake, va; and cranberry glades, wv) during several seasons. recovery of mais was significantly higher from waters, soils, and aerosols collected from the two acid, brown-water swamps located in th ...19921736730
characterization of immunosuppressive functions of murine peritoneal macrophages induced with various agents.murine peritoneal macrophages (m phi s), induced with stimuli such as thioglycollate, zymosan a, ok-432, bacille calmette-guérin (bcg), or live mycobacterium intracellulare, showed varying levels of inhibitory activity against the concanavalin a (con a) blastogenic response of splenic t cells. all test m phi s significantly inhibited the interleukin 2 (il-2)-producing ability of t cells but this inhibition was not enough to explain the observed reduction in t cell con a mitogenesis. in contrast, ...19921740643
fourth report of the cooperative, open-ended study of slowly growing mycobacteria by the international working group on mycobacterial taxonomy.the open-ended study of the international working group on mycobacterial taxonomy is an ongoing project to characterize slowly growing strains of mycobacteria that do not belong to well-established or thoroughly characterized species. in this fourth report we describe two numerical taxonomic clusters that represent subspecies or biovars of mycobacterium simiae, one cluster that encompasses the erstwhile type strain of the presently invalid species "mycobacterium paraffinicum," one cluster that i ...19911742195
[identification of mycobacterium avium and mycobacterium intracellulare using three dna probe tests, and their distributions in japan].identification of mycobacterium avium complex (mac) was made using three dna probe tests for mac: gen-probe rapid diagnostic system for the mac (gen-probe inc., san diego, u.s.a.), accuprobe mac culture identification or confirmation test (gen-probe inc.); and snap culture identification diagnostic kit (mac) (syngene inc., san diego, u.s.a.). various strains of mac belonging to serovars 21 to 28 were identified by the dna probe tests and showed the following. first, serovar 21 and 25 belonged to ...19911766154
paradoxical effect of tween 80 between the susceptibility to rifampicin and streptomycin and the susceptibility to ethambutol and sulfadimethoxine in the mycobacterium avium-mycobacterium intracellulare complex.the susceptibility to rifampicin and streptomycin of the mycobacterium avium-mycobacterium intracellulare complex was augmented by the addition of tween 80 into 7h10 agar medium with oadc, whereas the susceptibility to ethambutol and sulfadimethoxine was either not changed or reduced by the addition of tween 80. in 7h10 agar medium without oadc, however, susceptibilities to both rifampicin and sulfadimethoxine were reduced by the addition of tween 80 to the medium. a number of hypotheses are mad ...19911779893
[therapeutic efficacy of kanamycin and clofazimine combined with muramyl dipeptide against mycobacterium intracellulare infection induced in mice].therapeutic efficacy of kanamycin (km) and clofazimine (cfz) combined with n2-[(n-acetyl-muramyl)-l-alanyl-d-isoglutaminyl]-n6-stearoyl-l-ly sine, mdp-lys (l18), against mycobacterium intracellulare infection induced in mice was studied, based on suppression of incidence of gross lung lesions and bacterial growth at the sites of infection (lungs and spleen), and the following results were obtained. firstly, km (0.5 mg) was given sc to mice, daily six times per week in combination (or not) with s ...19911784098
characterization by restriction endonuclease analysis and seroagglutination of strains of mycobacterium avium and mycobacterium intracellulare obtained from farmed deer.restriction endonuclease analysis and seroagglutination were used to characterize strains of mycobacterium avium and mycobacterium intracellulare (collectively, mai) recovered from 1 local herd and 2 imported shipments of red deer (cervus elaphus) that developed sensitization to bovine tuberculin during skin testing. a total of 31 mai strains were isolated from lymph node pools (head, thorax, abdomen, and peripheral regions) of 21 of 29 local deer. similarly, 15 mai strains were isolated from th ...19911849382
[chemotherapeutic regimens that were considered effective to cure pulmonary infection caused by mycobacterium avium-mycobacterium intracellulare complex].during the period of 24 years from 1965 to 1988, we treated a total of 181 patients who had pulmonary infection caused by mycobacterium avium--mycobacterium intracellulare complex (mai complex). of these 181, 34 (19%) were cured showing sputum conversion and disappearance of cavity or marked reduction of cavity in the size to 1/2 or less or change of the cavity to thin-walled one. in these patients, negative culture continued at least for one year by monthly sputum examination. the most frequent ...19911875580
effect of tween 80 on formation of the superficial l1 layer of the mycobacterium avium-mycobacterium intracellulare complex.the effects of tween 80 supplementation of liquid culture medium on the formation of the superficial l1 layer of the mycobacterium avium-mycobacterium intracellulare complex (mac) were examined by serological and scanning electron microscopic experiments. specific antiserum to the glycopeptidolipids on the l1 layer of m. avium s-139, made in a rabbit, was used for seroagglutination reactions with antigens prepared from strain s-139 grown in medium supplemented with various levels of tween 80 (0, ...19911885740
[in vitro susceptibilities of mycobacterium avium and mycobacterium intracellulare to various drugs].mycobacterium avium and m. intracellulare isolated from patients infected with m. avium complex (mac), which were identified by gen-probe rapid diagnostic system for the mac, were studied for susceptibility to various antimicrobial agents, including rifampicin, rifabutin, kanamycin, streptomycin, amikacin, ethambutol, clofazimine, isoniazid, ofloxacin, ciprofloxacin and cycloserine. ratio of resistant strains to test strains to a given agent at prescribed concentration in cases of m. avium and m ...19911890791
therapeutic efficacy of liposome-encapsulated kanamycin against mycobacterium intracellulare infection induced in mice.liposome-encapsulated kanamycin (km) was examined for therapeutic efficacy against experimental infection induced with mycobacterium intracellulare in mice. liposomal km injections (once weekly for up to 8 wk) led to a greater reduction in the degree of gross pulmonary lesions and in the growth of the organisms in the visceral organs (lungs, liver, spleen, and kidneys) of infected mice than did either free km alone or free km mixed with empty liposomal vesicles. the therapeutic effect of the lip ...19911892297
[identification of mycobacterium avium, mycobacterium intracellulare, and mycobacterium tuberculosis complex by gen-probe rapid diagnostic system].dna probe testing for mycobacterium avium, mycobacterium intracellulare and mycobacterium tuberculosis complex (mtc) was performed using gen-probe rapid diagnostic system (gen-probe inc., san diego, calif., u.s.a.). by dna probe test carried out blindfold for 48 mycobacterial strains with code numbers obtained from kyoto university (prof. f. kuze), 13, 7, and 5 strains were identified as to be m. avium, m. intracellulare, and mtc, respectively. the diagnostic specificity and sensitivity of this ...19911909765
antigens of the mycobacterium avium, mycobacterium intracellulare complex.in the past decade, the clinical significance of the mycobacterium avium, myobacterium intracellulare complex (mac) has increased dramatically primarily because of the association between the mac and the acquired immunodeficiency syndrome (aids). recent hospital reports have suggested that about one-half of aids patients in the united states are infected with the mac. the resulting myobacteremia is a primary cause of mortality in 5-10% of these patients. this increased clinical importance of the ...19911915784
[enzymatic profile of mycobacterium avium and mycobacterium intracellulare].the characterization of extracellular enzymatic activities of mycobacterium avium and mycobacterium intracellulare which were identified by dna probe (gen-probe, cal., usa) was carried out using the api zym system (api, la balme les grottes, france). the enzymatic activities of m. avium were attributed to esterase (c4), esterase lipase (c8), leucin arylamidase, acid phosphatase and phosphoamidase. enzymatic characterization of m. intracellulare was very similar to that of m. avium. however, m. i ...19911921096
[reactivities of various mycobacteria species against dna probes (gen-probe rapid diagnostic system) specific to mycobacterium tuberculosis complex, mycobacterium avium and mycobacterium intracellulare].various mycobacterial species (22 species, 178 strains) were studied for their reactivity to dna probe specific for mycobacterium tuberculosis complex (mtc), m. avium or m. intracellulare, using gen-probe rapid diagnostic system (gen-probe inc., san diego, calif., u.s.a.). all the mtc strains, including m. tuberculosis, m. africanum, m. bovis and m. microti reacted with mtc-dna probe at the % hybridization value of 42.8-51.9% (values higher than 10% are regarded as positive), but their reactivit ...19911942722
analysis of cellular fatty acids and proteins by capillary gas chromatography and sodium dodecyl sulphate polyacrylamide gel electrophoresis to differentiate mycobacterium avium, mycobacterium intracellulare and mycobacterium scrofulaceum (mais) complex species.infections due to atypical mycobacteria have increased during the past 30 years. species of mycobacterium avium, mycobacterium intracellulare and mycobacterium scrofulaceum are among the most common non-tuberculous mycobacteria isolated from patients with aids or immunosuppressed. these three organisms are taxonomically closely related and identification, according to cultural characteristics and biochemical tests, is not always evident, so some of these related strains are grouped in a "mais" c ...19902084120
isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different mycobacterium intracellulare antigens.four bacteriophages expressing different immunoreactive recombinant mycobacterium intracellulare antigens were isolated from a lambda gt11 library with monoclonal antibodies to m. intracellulare. these four antibodies reacted with native m. intracellulare proteins of 54, 43, 40/38, and 22 kilodaltons. southern blot hybridizations with dna probes prepared from insert fragments of these bacteriophages confirmed the m. intracellulare derivation of the inserts. the physical maps of the immunoreactiv ...19902136733
identification of various serovar strains of mycobacterium avium complex by using dna probes specific for mycobacterium avium and mycobacterium intracellulare.reference strains of the mycobacterium avium complex (mac) belonging to serovars 1 to 28 were examined with dna probes (gen probe rapid diagnostic system for the mac; gen probe inc., san diego, calif.) specific for either m. avium or mycobacterium intracellulare. this study revealed that the earlier designations of the mac serovars, in which serovars 1 to 3 and 4 to 28 were regarded as m. avium and m. intracellular, respectively, should be revised as follows. first m. avium includes serovars, 1 ...19902203807
[determination of the susceptibilities to antituberculosis agents in mycobacterium avium-mycobacterium intracellulare complex].the determination of the susceptibilities to antituberculosis agents in mycobacterium avium-mycobacterium intracellulare complex (mai complex) is influenced by the size of inoculation used in the determination. such influences differ greatly according to the drugs. by increasing the number of colony-forming units (cfu) of the inoculation to 10(6)-fold, minimal inhibitory concentrations (mics) of rifampicin and cycloserine have increased only 2-to 3-fold. however, mics of ethionamide, streptomyci ...19902214509
[bactericidal activities of rifampicin, ethambutol, enviomycin and streptomycin on mycobacterium avium-mycobacterium intracellulare complex strains].bacteriostatic and bactericidal activities of rifampicin, ethambutol, enviomycin and streptomycin on mycobacterium avium-mycobacterium intracellulare complex (mai complex) strains were determined. the susceptibility testings were made in ogawa egg medium, and minimal inhibitory concentrations (mics) were determined as the lowest concentration of drugs, in which the growth of 20 to 100 colony-forming units was completely inhibited. the mics correspond to the mics that can inhibit the growth of 95 ...19902214513
epidemiology of infection by nontuberculous mycobacteria ix. evidence for two dna homology groups among small plasmids in mycobacterium avium, mycobacterium intracellulare, and mycobacterium scrofulaceum.a 12.9 kb plasmid, pvt2, from a clinical mycobacterium avium isolate, md1, was cloned and radiolabeled for use as a dna probe to examine the relatedness of plasmids in m. avium complex. that probe hybridized with plasmids isolated from m. avium complex strains from the environment (7 of 16) and from non-acquired immunodeficiency syndrome (aids) (10 of 17) and aids (5 of 6) clinical isolates. the similarity of plasmids from the environment with those from patients supports the hypothesis that the ...19902221593
arylsulfatase activity of mycobacterium avium, m. intracellulare, and m. scrofulaceum.a rapid (3-h) arylsulfatase assay for cell suspensions of mycobacteria, in which p-nitrophenyl sulfate is used as the substrate, was developed. arylsulfatase activity was found in cell suspensions of representative strains of mycobacterium avium, mycobacterium intracellulare, and mycobacterium scrofulaceum grown without the substrate in either middlebrook 7h9 medium containing 0.2% (wt/vol) glucose and 0.05% (vol/vol) tween 80 or dubos broth medium, but was absent in cells grown in a low-ph, min ...19902223599
arylsulfatase activity for differentiating mycobacterium avium and mycobacterium intracellulare.arylsulfatase activities (96-h reaction) of various strains of mycobacterium avium and m. intracellulare, as identified by a dna probe test, were measured. the enzyme activities of m. avium strains were significantly lower [corrected] than those of m. intracellulare strains (p less than 0.005 to p less than 0.025). the enzyme activities did not vary with serovar; that is, the activities of serovars 1, 2, 8, and 9 (belonging to m. avium) were similar to each other, as were the activities of serov ...19902229391
tuberculous psoas muscle abscess following chemoprophylaxis with isoniazid in a patient with human immunodeficiency virus infection.a 34-year-old man with human immunodeficiency virus infection and disseminated mycobacterium avium and mycobacterium intracellulare infection developed a right psoas muscle abscess due to mycobacterium tuberculosis. the abscess occurred 18 months after completion of a 12-month course of chemoprophylaxis with isoniazid that was given because of a positive reaction with purified protein derivative of tuberculin. the adjacent vertebrae did not appear to be involved. the abscess was drained with a p ...19902237113
in-vitro bacteriostatic and bactericidal activity of isoniazid on the mycobacterium avium-mycobacterium intracellulare complex.isoniazid inhibited the growth of mycobacterium avium-mycobacterium intracellulare strains at concentrations of 0.1-25 micrograms/ml and was even bactericidal for several strains. the bactericidal activity was observed in relatively susceptible strains. the susceptibility did not correlate with colonial morphology but correlated with species identification. mycobacterium avium strains were more resistant to isoniazid than mycobacterium intracellulare strains.19902238126
[clinical characteristics of the lung diseases due to mycobacterium avium and mycobacterium intracellulare classified by dna probe test].clinical characteristics of the lung diseases due to m. avium and m. intracellulare classified by dna probe test were investigated. between m. avium and m. intracellulare, there was no significant differences in the samples' backgrounds and the clinical characteristics except for their prognoses. the prognosis of the lung diseases due to m. intracellulare was better than those due to m. avium, and m. avium was revealed to be highly susceptible to cyclocerine than m. intracellulare. no remarkable ...19902259056
usefulness of skin testing with mycobacterial antigens in children with cervical lymphadenopathy.one hundred twenty-three children with chronic cervical lymphadenopathy were skin-tested with purified protein derivative (ppd)-b (mycobacterium intracellulare), ppd-y (mycobacterium kansasii), ppd-g (mycobacterium scrofulaceum) (nontuberculous mycobacterial antigens (ntmags)) and ppd-t (mycobacterium tuberculosis). children with culture-confirmed mycobacterial disease had significantly larger reactions to ntmags and were 6 times more likely to have ppd-b responses of greater than or equal to 10 ...19921608681
synthesis and biological activity of 5'-aminobenzoxazinorifamycin derivatives.benzoxazinorifamycin reacted with various secondary amines to yield various 5'-substituted aminobenzoxazinorifamycin derivatives. the derivatives exhibited potent activities against gram-positive bacteria and mycobacteria. the antimicrobial activities of these compounds against mycobacterium tuberculosis and mycobacterium intracellulare were superior to those of rifampicin. some of these compounds showed good plasma levels after oral administration in rats.19921464100
[counting efficacies of the cfu-enumerating method and microscopic counting method for mycobacteria located in cultured macrophages].i compared counting efficacies of cfu-enumerating method for the number of mycobacteria (mycobacterium intracellulare and m. fortuitum) locating in cultured macrophages with that of microscopic counting method. zymosan a-induced macrophages from ddy mice were infected with either m. intracellulare or m. fortuitum by incubation in 10% fbs-rpmi 1640 medium containing the organisms for 1 hr, thereafter thoroughly washed to remove extracellular bacilli, and cultured for 3 to 5 days. at intervals, ma ...19921453568
chronic arthritis due to mycobacterium intracellulare. sacroiliac, knee, and carpal tunnel involvement in a young man and response to chemotherapy. 1976942507
[serotyping of strains of non-tuberculous mycobacteria].forty strains of mycobacteria, belonging to mycobacterium-avium-intracellulare-scrofulaceum complex, isolated from symptomatic respiratory patients, were studied. for such study, agglutination-adsorption technique was applied, using specific antisera elaborated at the "pedro kouri" institute of tropical medicine, national reference institute, with titers ranging close to 1:320. the results obtained demonstrated that the prevailing types were those of the species mycobacterium intracellulare (31 ...19902259778
phylogenetic analysis and identification of different serovars of mycobacterium intracellulare at the molecular level.comparative 16s rrna sequencing was used to infer the phylogenetic relationship among different serovars of the mycobacterium avium-m. intracellulare complex as well as to define signature nucleotides characteristic for different serovars. in general, the groups defined by rrna sequencing reflect the classification obtained with sensitin tests and pathogenicity examinations in chickens. unique 16s rrna sequence patterns could be defined for (1) m. avium, (2) m. intracellulare serovars 4, 5, 6, 8 ...19902275731
production, characterization, and species specificity of monoclonal antibodies to mycobacterium avium complex protein antigens.the incidence of mycobacterium avium-mycobacterium intracellulare complex infections has increased in recent years primarily because a significant proportion of acquired immunodeficiency syndrome patients develop disseminated m. avium complex disease. in an effort to develop new tools to study these infections, we have produced eight monoclonal antibodies directed against m. avium. western blot (immunoblot) specificity analysis and protease sensitivity assays indicate that four of these antibodi ...19902323822
implications of antibodies to pyruvylated glucose in healthy populations for mycobacterioses and other infectious diseases.members of the mycobacterium avium-mycobacterium intracellulare (mai) complex are typeable because each serovar is characterized by its own specific antigenic glycolipid. by means of an enzyme-linked immunosorbent assay, we studied serum specimens obtained from 148 healthy college students for antibodies to these glycopeptidolipids. ninety-two (61.5%) of the serum specimens were positive to the specific glycolipid antigen from mai serovar 8. in a study of a pediatric population, antibodies appea ...19902332469
[utilization of butanols by disease-associated mycobacterium avium and mycobacterium intracellulare classified by dna probe test and relationship with their ability to grow at 45 degrees c].utilization of n-butanol and iso-butanol by mycobacterium avium and m. intracellulare classified by dna probe test (20 strains each) was studied. the majority (14 strains) of m. avium could grow at 45 degrees c, but none of m. intracellulare grew at the same temperature. all m. avium and the majority of m. intracellulare (80%) utilized n-butanol but failed to utilize iso-butanol. therefore, the utilization test of butanols is inadequate for classification of these species.19902376935
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