| studies on antituberculotic action of some phenothiazine derivatives in vitro. | five phenothiazine derivatives (chlorpromazine, levomepromazine, diethazine, promethazine and chlorpromazine) sulphoxyde were tested for antimycobacterial activity. the growth of mycobacterium tuberculosis, m. bovis and m. butyricum was inhibited by chlorpromazine practically at identical concentrations. the minimum inhibitory concentrations for m. tuberculosis were: chlorpromazine and levomepromazine, 10 microgram/ml; diethazine and promethazine 20 microgram/ml, whilst chlorpromazine sulphoxyde ... | 1977 | 24964 |
| effect of immunomodulators on thymocyte suppressive activity in mice. | | 1978 | 28950 |
| activation of macrophages assessed by in vivo and in vitro tests. | | 1979 | 44641 |
| the effect of toxoplasma cell fractions and mycobacterial immunostimulants against virulent toxoplasma gondii in mice. | toxoplasma gondii tachyzoites were disrupted in a ribi cell fractionator and separated into cell walls and protoplasm by differential centrifugation. these products were used alone or combined with a mycobacterial glycolipid (p3) and injected either as oil-in-water emulsions or incorporated in freund's incomplete adjuvant. mice were vaccinated by intravenous or intradermal routes and challenged intraperitoneally with a highly virulent strain of toxoplasma gondii. a local granuloma formation was ... | 1979 | 44793 |
| a possible relationship between delayed hypersensitivity and cell-mediated immunity. | | 1975 | 47728 |
| investigation into the relationship of m ulcerans to m. buruli and other mycobacteria. | the main characteristics of mycoplasma ulcerans are its remarkably distinct drug sensitivity pattern and its rather poor enzymatic activity. m. microti is clearly distinguishable from m. bovis and m. tuberculosis. some of the m. buruli strains appear to be identical with m. ulcerans, whereas others show similarity either to m. microti or to m. tuberculosis. it seems that m. buruli is not a legitimate species. | 1975 | 47730 |
| comparison of the specificty of human and bovine tuberculin ppd for testing cattle. 1--republic of ireland. | a tuberculin testing trial in cattle was carried out in the republic of ireland to compare the specificity for bovine tuberculosis of a human purified protein derivative (ppd) tuberculin (weybridge) with that of a bovine ppd (rotterdam), and to determine whether discrimination between specific and non-specific reactions to mammalian tuberculin is better with doses of tuberculins smaller than those traditonally used for testing cattle. tests were carried out in 510 cattle, 395 of which were shown ... | 1975 | 47750 |
| effect of infection with trichinella spiralis and bcg on thymic histology. | the histology of mouse thymic tissue was examined following infection with trichinella spiralis and/or bcg. icr/cd-1 mice were orally infected with 200 t. spiralis 56 days preceding intravenous administration of 4x10-6 viable bcg. following assessment of delayed hypersensitive foot pad reactions to bcg, the mice were bled and sacrificed. the thymus was removed from each animal, fixed in 10% neutral buffered formalin, embedded in paraffin and cut in 4 mum sections. examination of serial sections ... | 1975 | 49304 |
| chemotherapy of gastrointestinal cancer. | | 1975 | 61195 |
| a system for the examination of tubercle bacilli and other mycobacteria. | methods are described for the examination of mycobacteria cultured from clinical specimens. in the "screening" procedure used for new isolates tubercle bacilli are non-pigmented, do not grow at 25 degrees c and are sensitive to p-nitrobenzoic acid as well as normally to anti-tuberculosis drugs. classification is extended when necessary by the use of four tests--temperature requirements, pigmentation, oxygen preference and tween hydrolysis. these define 15 species or groups meeting the needs of c ... | 1976 | 62439 |
| the effects of bcg on both cellular and humoral immunity during the early response to a hapten carrier complex. | cell-mediated reactions to the carrier and antibody-mediated reactions to hapten and carrier were studied in guinea-pigs treated with a single i.v. injection of bcg at different intervals of time before immunization with two different doses of a hapten-carrier complex. the results clearly show that bcg-induced delayed hypersensitivity reactions to the carrier and decreased antibody synthesis to the hapten. it is proposed that bcg acts on a dispatcher cell which controls both cellular and humoral ... | 1976 | 63343 |
| immunological and other laboratory studies of patients receiving short-term cimetidine therapy. | | 1977 | 64654 |
| experimental cancer immunotherapy: modification of tumor cells to increase immunogenicity. | firmly established transplantable c3h/hej mammary carcinomas can be inhibited by host challenge with vibrio cholerae neuraminidase (vcn)-treated tumor cells. the effect is totally immunospecific, even vcn-treated tumors bearing shared mammary tumor virus (mtv) antigen cannot induce the regression. thus, vcn is capable of increasing the immunogenicity of the private, unique-unshared tumor antigens on mammary carcinomas; vcn is incapable of increasing the immunogenicity of the shared mtv-associate ... | 1976 | 68699 |
| transfer of delayed hypersensitivity in mice to microbial antigens with dialyzable transfer factor. | dialyzable lawrence-type transfer factor was prepared from the spleen cells of cf1 mice inoculated with coccidioides immitis- and candida albicans-killed vaccines and with live mycobacterium tuberculosis vaccine (bcg). these preparations were shown to transfer antigen-specific cell-mediated immunity to naive mice, as measured by the delayed skin test and footpad-swelling methods. reactivity could be demonstrated when the test antigens were given 24 h after the transfer factor, but not when they ... | 1977 | 68930 |
| precipitation of radiolabeled antigen-antibody complexes with protein a-containing staphylococcus aureus. | the feasibility of using protein a-containing staphylococcus aureus to measure antibodies in sera from several mammalian species was studied. a variety of unrelated radiolabeled antigens were tested, including components of bovine serum, dna, and bacterial and tumor-associated extracts. the use of s. aureus was found to be a reliable way to detect and measure the primary interactions between many of the antigens and antibodies tested. results were equivalent under many circumstances to those obt ... | 1977 | 68969 |
| stimulation and inhibition of macrophages prior to immunization with a hapten-carrier conjugate. | delayed hypersensitivity reactions and antibody-mediated reactions to the carrier and to the hapten have been studied in animals treated with bcg or/and trypan blue before immunization with a hapten-carrier complex. the results show that it is possible to induce, with bcg, at the beginning of the response, an antigenic competition between hapten and carrier moieties of the antigen molecule. macrophages which modulate the response to the hapten-carrier complex by acting both on t cells involved i ... | 1977 | 75840 |
| an attempt at the use of immunological methods for the detection of enzymes of bcg substrains. | immunological methods have been used to visualize peroxidase and dehydrogenase activities of substrains of bcg mycobacteria. immunodiffusion (id) and immunoelectrophoresis (ie) in agar gel and two-dimensional immunoelectrophoresis (2d-ie) in agarose gel were employed. in all these tests part of the id, ie, and 2d-ie preparations were stained with amido black 10b (ab 10b) to visualize the total number of precipitin lines and part were stained at the same time with histochemical methods for vizual ... | 1978 | 76421 |
| the use of two-dimensional immunoelectrophoresis for the characterization of antigens of bcg substrains. | the usefulness of the method of two-dimensional immunoelectrophoresis (2d-iep) in comparative studies and characterization of the antigenic spectra of various bcg substrains have been estimated. the bcg substrains used were: substrain bcg-rio de janeiro, substrain bcg-poland, form-rough (r) and substrain bcg-poland, form-smooth (s). the 2d-iep technique was found to give characteristic immunoelectrophoretic patterns of antigenic composition of the different bcg substrains. the number of precipit ... | 1978 | 76422 |
| requirements for induction of delay hypersensitivity in the chicken. | | 1978 | 76579 |
| macrophage disappearance reaction in rana esculenta induced by specific antigen and rabbit lymphokines. | macrophage disappearance reaction was induced by intraperitoneal injections of specific antigens in rana esculenta sensitized by bovine gamma globulin and mycobacterium bovis bcg. rabbit lymphokines derived from concanavalin a stimulated blood lymphocytes injected intraperitoneally were able to induce macrophage disappearance reaction in normal rana esculenta. this suggests that mammalian lymphokines are capable of acting in amphibia. mammalian lymphokines have not an exclusive class-specificity ... | 1977 | 73345 |
| use of sepharose-conjugated bcg antibodies for the purification of tuberculin-active components. | bacillus calmette-guérin (bcg) antibodies conjugated with cnbr-activated sepharose 4b led to the isolation of tuberculin-active components from the protoplasmic materials of bcg. from these results; it is concluded that most of the tuberculin-active components (sensitins) have mycobacterial antigenic properties. | 1977 | 68806 |
| antitumor activity of mycobacterial glycolipid a1. | glycolipid a1 isolated from mycobacterium bovis bcg, when dissolved in olive oil and injected together with line 10 transplantable hepatoma cells, is able to elicit a host response which results in the abrogation or retardation of tumor growth in syngeneic guinea pigs. glycolipid a1 does not have adjuvant activity for delayed type hypersensitivity, and antibodies to a1 have not been detected in the sera of guinea pigs during or after the tumor abrogation induced by a1 injection glycolipid a1 doe ... | 1978 | 81809 |
| the effects of certain immunity systemic advuvants, pha, and human gamma globulin on the thymic cortex of mice: a light and electron microscope study. | thymus of (c57bl/6 x dba/2) f1 mice was examined histologically, histochemically and ultrastructurally, seven days after intravenous injection of bcg, pertussis vaccine, lipopolysaccharide or human gamma globulin, or intraperitoneal injection of complete or incomplete freund's adjuvants or of phytohemagglutinin. only bcg induced a marked increase of the secretory activity of the thymic epithelium at all histological sites (cortex, corticomedullary junction and medullar). only with this adjuvant ... | 1976 | 66071 |
| comparison between the effects of micrococcus, bcg and related polysaccharides in preclinical chemo-immunotherapy of murine l 1210 leukaemia [proceedings]. | | 1978 | 84653 |
| [adjuvant immunotherapy with bcg for malignant brain tumors (author's transl)]. | | 1976 | 62316 |
| [experiments for the serological establishment of the species of mycobacteria inducing an immune restructuring of the macroorganism]. | serological investigations are carried out on hyperimmune rabbit and guinea pig sera with two antigens each of mycobacterium tuberculosis h37rv, mycobacterium bovinus, mycobacterium avium and mycobacterium kanzassi. one of the antigens is obtained through electrohydraulic destruction of delipidated mycobacteria, while the other constitutes cell walls, purified with ribonuclease, of non-delipidated mycobacterial cells, destroyed by the same method. the antigens, prepared by the electrohydraulic m ... | 1978 | 87060 |
| studies of leukemia specific antigen and immunotherapy of acute leukemia with cell-wall skeleton of bcg. | | 1978 | 87101 |
| [effect of pesticides on tissue structures of the lungs and on the course of experimental tuberculosis]. | | 1979 | 89674 |
| peroxidase staining in elicited and nonelicited mononuclear peritoneal cells from bcg-sensitized and nonsensitized mice. | the peroxidase (po) activity in nonelicited macrophages and in casein-elicited monocytes, obtained from peritoneal cavities of nonsensitized and bcg-sensitized mice and cultivated on glass for 1 or 2 h, was studied by light and electron microscopy, using the 3,3'-diaminobenzidine technique. these two types of glass-adherent peritoneal cells differed in po activity. in macrophages, po activity was predominantly in the nuclear envelope, rough endoplasmic reticulum, and occasionally in vesicles of ... | 1976 | 61177 |
| specificity of antigenic fractions of tuberculin. | purified protein derivative from mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 v/40 ma in ph 4.2 acetic acid-pyridine buffer. further purification of one of the fractions on sephadex g 25 column and by acid hydrolysis yielded antigen "ps" eliciting tuberculin reaction only in animals vaccinated with m. bovis bcg but not in those sensitized with m. avium and some fast-growing mycobacteria. the specificity of antigen "ps" was confirmed in vitro: the antigen induced ... | 1976 | 59518 |
| effect of micrococcus, bcg and structurally related polysaccharides on the adjuvanticity to a t cell-dependent antigen [proceedings]. | | 1979 | 93950 |
| [gram-positive bacteria as adjuvants (author's transl)]. | gram-positive bacteria or their constituents influence the immune response in various ways. for instance: listeria monocytogenes cellwall fraction acts as b-cell mitogen, in this way enhancing a humoral immune response. corynebacterium parvum acts as a macrophage stimulant evoking maximum macrophage activation. bcg, among other things, leads to an enhancement of a t-cell response to unrelated antigens. the hypothetical therapeutic consequences are discussed. | 1978 | 96006 |
| test for differentiation of m. tuberculosis and m. bovis from other mycobacteria. | a test is described for selective inhibition of mycobacterium tuberculosis and m. bovis isolates in fluid medium. the method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (nap) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other mycobacteria. | 1978 | 96930 |
| mycolic acids. a reinvestigation. | mycolic acids derived from the cell walls of mycobacterium bovis bcg, mycobacterium bovis bovinus i, mycobacterium smegmatis, and mycobacterium tuberculosis h37rv have been fractionated as their p-bromophenacyl esters by a two-step high performance liquid chromatographic procedure: 1) adsorption chromatography on 10-micrometer particle size silica gel, and 2) reverse phase partition chromatography on a 10-micrometer particle size support containing a c18 bonded phase. this procedure has resulted ... | 1978 | 97301 |
| shared antigens between human malignant melanoma cells and mycobacterium bovis (bcg). | this study was undertaken to investigate the antigenic relationships between human malignant melanoma cells and mycobacterium bovis (bcg). rabbits were immunized with sonicates of bcg or with malignant melanoma cells from different patients and the resulting antisera were tested for their capacity to bind radiolabeled soluble extracts prepared from bcg and melanoma cells. the binding of antibodies to radiolabeled antigens was studied by precipitation of radiolabeled antigen-antibody complexes by ... | 1976 | 58033 |
| [method of increasing the effectiveness of the passive phosphatide hemagglutination test]. | | 1978 | 97659 |
| enhancing effect of passive immunization with mycobacterial antibodies on humoral and cellular immunity in bcg-infected mice. | the present paper is an attempt to get insight about the mechanisms involved in the enhancing phenomenon that occurs in bacterial infections. mice infected with small doses of the bcg strain of m. bovis and treated with a m. tuberculosis h37rv antiserum investigated at various intervals for their capacity to synthesize haemagglutinating antimyco-bacterial antibodies and to develop delayed hypersensitivity to ppd. as controls, bcg-infected mice were treated with the antiserum freed from its mycob ... | 1978 | 98097 |
| bacteria induce lymphokine synthesis polyclonally in human b lymphocytes. | we have studied the ability of various bacteria to stimulate human lymphocytes to produce leukocyte migration inhibitory factor (lif). mononuclear cells from adult and cord blood as well as purified t and b lymphocytes were stimulated with killed bacteria. the culture supernatants were tested for the presence of lif by the agarose migration method. all nine bacterial strains tested activated unseparated mononuclear cells and b lymphocytes but not t cells to produce lif. lif was also present in c ... | 1978 | 98583 |
| partial purification of a serum factor that causes necrosis of tumors. | tumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus calmette-guérin and given bacterial endotoxin. sera from normal mice, endotoxin-treated mice, and mice infected with bacillus calmette-guérin do not contain this factor. a 20- to 30-fold purification of the serum factor has been achieved by (nh4)2so4 fractionation, sephadex g-100 and g-200 gel filtration, and preparative polyacrylamide elec ... | 1976 | 54919 |
| [antitumor activity of the bacterial walls of br. abortus 99]. | a brief chronologic survey is made of investigations that reveal that the bacterial cell walls and their chemical components exhibit antitumoral activity. discussed is the problem which are the constituents of the bacterial wal and what is the mechanism through which such effects are produced. the conclusion is drawn that some peptides participating in the structure of the bacterial wall after their release in body stimulate the macrophages and thus confer antitumoral activity to them. | 1978 | 98899 |
| depression of protective mechanisms against microörganisms in tumor-bearing mice and its restoration by adjuvants. | bacterial growth within 72 hr after an intravenous inoculation with listeria monocytogenes was enhanced strikingly in the liver of mice, when viable cells of sarcoma-180 were injected subcutaneously into ddn, c3h/he, and balb/c mice 5 hr before the inoculation. such an enhanced bacterial growth appears to be attributable to a depressed ability of macrophages to digest engulfed bacteria. pretreatments with zymosan, killed bcg, or viable bcg prevented such depression in tumor-bearing mice and incr ... | 1977 | 99344 |
| [the complement fixation test with bcg in the diagnosis of leishmaniasis]. | the utility of complement-fixation test with an antigen from commercially lyophilized bcg was investigated in the diagnosis of leishmaniasis. the following results were obtained: visceral leishmaniasis: 4 positivity on 5 cases; oriental sore: 3 positivity on 6 cases; canine leishmaniasis: 6 positivity, at high level, and 2 negativity; other diseases: 5 positivity, on 17 sera of tbc; no positivity in sera from patients with various diseases, in control and in normal dogs. the results are compared ... | 1975 | 54183 |
| multiple in vitro mechanisms of tumor cytotoxicity demonstrated in the line-1 guinea pig hepatoma model. | the line-1 guinea pig hepatoma was used to study in vitro tumor cytotoxicity. cytotoxicity was determined by measurement of the loss of tritiated thymidine-labeled target cells from culture vessels. with this technique, we demonstrated that significant tumor cytotoxicity was caused by lymphoid cells from tumor-immune guinea pigs, by cells from guinea pigs immunized against an antigen urelated to the tumor target, and by cell-free supernatants rich in lymphocyte mediators. addition of normal peri ... | 1975 | 52720 |
| effect of oil-attached bcg cell-wall skeleton and thymectomy on the incidence of lung cancer and amyloidosis induced by chemical carcinogens in rabbits. | intravenous injection of oil-attached bcg cell-wall skeleton showed potent preventive activity for induction of lung cancer by the intrabronchial instillation of chemical carcinogens (3-methylcholanthrene, 4-nitroquinoline 1-oxide) in rabbits. on the other hand, the thymectomized rabbits developed lung cancer by instillation of chemical carcinogens at an 80% incidence, compared to 47.2% in the controls. there were no differences in the appearance of amyloidosis between thymectomized and bcg cell ... | 1978 | 101411 |
| editorial: bovine tuberculosis and badgers. | | 1975 | 50520 |
| [biological properties of selected drug-resistant strains of mycobacterium tuberculosis]. | | 1978 | 101985 |
| the immunological aspects of malignant disease. | | 1975 | 49889 |
| comparison of the specificity of human and bovine tuberculin ppf for testing cattle. 3. national trial in great britain. | a field trial on a country-wide basis was undertaken to compare the specificity for bovine tuberculosis of single and comparative tuberculin tests in cattle using either weybridge human or weybridge bovine ppd. the tests were made on 10,305 cattle in 179 herds distributed throughout all regions of england, scotland and wales. results showed that a comparative tuberculin test using avian ppd with either human or bovine ppd had a much higher efficiency than a single injection of mammalian tubercul ... | 1975 | 47752 |
| immunological method to differentiate between antigens of tubercle bacilli, other mycobacterial species, and non-acid-fast bacteria. | sera from rabbits immunized with sonicates of mycobacterium bovis bcg were passed through an immunoadsorbent made of a soluble bcg extract to make partially purified antibodies to bcg. these antibodies were in turn used to prepare an immunoadsorbent through which the bcg extract was passed. the partially purified antigenic material was radiolabeled and subjected to electrophoresis in acrylamide gels. one of the radiolabeled fractions isolated (bcg-c) was found to bind to antibodies to bcg and h3 ... | 1978 | 103823 |
| comparison of the specificity of human and bovine tuberculin ppd for testing cattle. 2. south-eastern england. | a tuberculin testing trial was carried out in eight counties of south-eastern england to compare the specificity for bovine tuberculosis of weybridge human ppd with that of rotterdam bovine ppd. the matching of these two tuberculins for potency in naturally infected cattle had already been established, the bovine ppd being approximately one-and-a-half times more potent than the human ppd per unit of weight. in 1110 cattle in 25 herds with histories of long-standing freedom from tuberculosis and ... | 1975 | 47751 |
| [action of preparation k and its components on the causative agent of tuberculosis]. | | 1978 | 104131 |
| [lymph node tuberculosis--today]. | the peripheral tuberculosis of the lymph nodes on the neck, in the axilla and on the groin is described in its present epidemiological situation, its pathogenesis, diagnosis and therapy. nowadays the tuberculosis of the lymph nodes most frequently occurs at older age and in women. in most cases the m. tuberculosis is the causative organism. the differential diagnosis must take into consideration many possibilities and sources of error--also for the following therapy and expert opinion--, in most ... | 1978 | 104459 |
| non-specific induction of increased resistance in mice to trypanosoma congolense and trypanosoma brucei by immunostimulants. | administration of the immunostimulants corynebacterium parvum, bacillus calmette-guérin (bcg) or bordetella pertussis prior to, or at the same time as, challenge with trypanosoma congolense significantly increased survival times in mice, both of trypano-susceptible (a/j) and trypano-resistant (c57bl) strains. the increased survival time was associated with significant alterations in parasitaemia, which included lengthening of the pre-patent period, a delay in the time taken to reach the first pe ... | 1979 | 44360 |
| interferon and cytotoxic factor (cytotoxin) released in the blood of mice infected with mycobacterium bovis bcg. i. enhanced production of interferon and appearance of cytotoxin stimulated by capsular polysaccharide of klebsiella pneumoniae or bacterial lipopolysaccharide. | interferon production stimulated by the active substance (neutral fraction) of the capsular polysaccharide of klebsiella pneumoniae (neutral cps-k) in bcg-infected mice was compared with that by bacterial lipopolysaccharide (lps). prior infection with bcg increased the responsiveness of mice to the lethal effect of neutral cps-k as well as to that of lps. associated with this, bcg-infected mice showed a markedly enhanced ability to produce interferon after stimulation not only by lps but also by ... | 1979 | 41163 |
| superoxide production in pulmonary alveolar macrophages and killing of bcg by the superoxide-generating system with or without catalase. | the superoxide production of bcg-infected and noninfected alveolar macrophages was measured by superoxide dismutase-inhibitable nitro blue tetrazolium reduction. the cells were incubated with or without cell-free bronchial lavage fluid (pulmonary washings). when control alveolar macrophages were infected by bcg, superoxide production was decreased markedly, probably due to bacterial cytotoxic factors. in contrast, the production of superoxide in alveolar macrophages exposed to pulmonary washings ... | 1979 | 37165 |
| bovine tuberculosis. | | 1978 | 105166 |
| antimycobacterial activity of lecithin-cholesterol liposomes in the presence of phospholipase a2. | tubercle bacilli were preincubated with lecithin-cholesterol liposomes to be subsequently exposed to phospholipase a2. after further incubation in the environment of acidic buffer, viable units in the final mixture were enumerated by inoculating the serial dilutions of an aliquot onto kirchner agar medium containing horse serum in 5%. another aliquot was used for lipid analyses to confirm hydrolysis of lecithin. in addition to this bactericidal type of experiments, bacteriostatic tests were also ... | 1978 | 32413 |
| of mice and men in bone marrow transplantation. | | 1977 | 17189 |
| graft-versus-host reaction-like phenomenon induced by bcg in mice lethally irradiated and transferred with syngeneic bone marrow cells. | | 1977 | 16697 |
| immunity to mycobacterium leprae infections in mice stimulated by m. leprae, bcg, and graft-versus-host reactions. | infections of mice with mycobacterium leprae in one rear foot pad immunized them against a second infection in the other rear foot pad. purified bacilli harvested from the first infection also produced immuniy when injection into the foot pads of previously uninfected mice. injections of bcg afforded similar protection, but had no adjuvant effect on m. leprae. m. duvali, a cultivable mycobacterium that is reported to be more closely related antigenically to m. leprae than bcg is, provided much l ... | 1976 | 11189 |
| immunodiffusion studies of ribosomes in classification of mycobacteria and related taxa. | ribosomal preparations consisting of crude ribosomes (cr), 30s subunits (30s) and 16s core particles (16s) from four strains of the species mycobacterium bovis (bcg), mycobacterium fortuitum, mycobacterium phlei and mycobacterium smegmatis were analyzed by immunodiffusion technique for taxonomical purposes. the ribosomal preparations tested contained several interspecies cross-reacting precipitinogens. the number of precipitinogens demonstrated at the homologous reactions was generally larger th ... | 1979 | 109401 |
| purification of plasma membrane from bcg-induced rabbit alveolar macrophages. | | 1976 | 7672 |
| studies on the morphology of colonies of bacilli of bcg-poland substrain. i. the influence of iron on the type of bcg-poland colonies. | the influence of iron concentration in sauton's medium solidified with agar on the type of colonies of bcg-poland substrains, bcg-rio de janeiro, bcg-france, bcg-denmark and bcg-japan substrains has been examined. of all the studied bcg substrains only the bcg-poland substrain formed rough (r) and smooth (s) colonies. in the investigated substrains rough colonies became smaller with the decrease of iron concentration but they retained their characteristic surface roughness. the smooth colonies w ... | 1978 | 81600 |
| further studies of mycobacteriophage 33d (warsaw) for differentiation of bcg from m. bovis and m. tubeculosis. | mycobacteriophage 33d (warsaw) was used to differentiate bacille calmette-guerin (bcg) strains from m. bovis and m. tuberculosis. known strains as well as clinical strains of bcg were used in the study. single plaque isolations and adsorption studies demonstrated that phage 33d (warsaw) did not adsorb to bcg cultures. | 1979 | 109978 |
| comparison of micrococcus, bcg and related polysaccharides in the specific and non-specific immune resistance to murine l1210 leukemia [proceedings]. | | 1978 | 81034 |
| in-vitro immunological assay for diagnosis of coeliac disease. | | 1978 | 76821 |
| studies on the morphology of colonies of bacilli of bcg-poland substrain. ii. dissociation of bcg-poland substrain on löwenstein-jensen egg medium. | the dissociation of colonies of the substrain bcg-poland on löwenstein-jensen medium has been studied. it had been stated previously (lipińska and rzucidło, 1974) that bcg-poland substrain undergoes a process of dissociation which manifests itself in the appearance of smooth colonies alongside typical rough colonies. attempts have been made to obtain pure culture of both colony types. pure cultures composed of smooth or rough colonies respectively were obtained. the morphology and diameter of th ... | 1978 | 81601 |
| [bactericidal effect of disinfectants on various strains of mycobacterium tuberculosis]. | | 1979 | 113848 |
| self-inoculation with mycobacterium tuberculosis weiszfeiler w-115. | after intracutaneous self-inoculation with 0.05 mg (wet weight) of mycobacterium tuberculosis w-115 containing approximately 600 000 viable units, the local reaction was observed during a period of 9 weeks. no adverse effects or general reaction were observed. from the lesion the w-115 strain was recultivated. it was labelled w-115(78)st vaccine strain and is used for further studies. on the basis of experiments on laboratory animals, monkeys, human new-borns and adults, the strain w-115 is cons ... | 1979 | 113990 |
| [transmissible modification induced in mycobacterium tuberculosis and mycobacterium bovis in vitro]. | a biologically active material (fraction "s") is isolated from cultures of scotochromogenic mycobacteria. mycobacterium tuberculosis, or mycobacterium bovis by disrupting the cells, sedimentation through 2.2 m sucrose, and ultrafiltration. the fraction "s" induces the modification of tubercle bacilli into non acid-fast bacteria forming smooth colonies on nutritive glycerol agar within 24-36 h of incubation. three new phenotypes are thus obtained; two proved to be stable upon subculturing. freque ... | 1979 | 114293 |
| deoxyribonucleic acid relatedness among species of slowly-growing mycobacteria. | dna-dna hybridization is a reliable method for determining the phylogenetic relationship between bacterial strains the hybridization kinetics for dna from different slowly-growing mycobacteria were measured optically in a spectrophotometer. the results indicate that m. tuberculosis and m. bovis belong to one species. m. avium and m. intracellulare are two species, but some serotypes, now designated m. intracellulare, actually belong to m. avium. | 1979 | 115225 |
| comparison of various preparations of mycobacterium leprae and other mycobacteria by lymphocyte stimulation. | peripheral blood lymphocytes were stimulated in vitro with different mycobacterial antigen preparations and responses were measured as incorporation of tritiated thymidine. blood donors were 9 patients with lepromatous leprosy, 16 tuberculoid leprosy patients, and 6 healthy individuals with different degrees of exposure to leprosy. the results revealed a good correlation between the responses to m. leprae from human sources and bacilli from armadillos inoculated with human leprosy bacilli, altho ... | 1978 | 83309 |
| immunotherapy models in experimental animals. | | 1979 | 87018 |
| histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections. | histochemical staining for enzymes is usually performed on frozen sections. this report lists the longer incubation times required to demonstrate esterase, acid phosphatase, beta-galactosidase, and cytochrome oxidase in plastic embedded and ruotine paraffin embedded tissues. the sections embedded in plastic, i.e. water soluble methacrylate (polyscience's jb-4) and cut at 2 micrometers, were far superior to frozen sections and paraffin embedded sections both in tissue detail and in the localizati ... | 1979 | 90411 |
| [characteristics of the mycobacteria isolated from urogenital tuberculosis patients]. | | 1979 | 116222 |
| the methanol extraction residue of bacillus calmette-guerin protects against 7,12-dimethylbenz(alpha)anthracene-induced rat mammary carcinoma. | | 1979 | 117451 |
| kinetics of detection of blastogenic responses of neonatal calves inoculated in utero with tetanus toxoid, killed mycobacterium bovis, and killed brucella abortus. | nine pregnant cows were laparotomized and their fetuses were immunized with tetanus toxoid, killed brucella abortus, and killed mycobacterium bovis. blastogenesis assays and total leukocyte and differential counts were done when the calves were 1, 2, 3, 7, 14, 21, 28, and 60 days of age. initial blastogenesis responses to antigens, phytohemagglutinin, and concanavalin a were not positive as frequently as were the responses obtained when the calves were 2 to 3 weeks of age. the probability of obt ... | 1979 | 117730 |
| a practicable immunological approach to block spermatogenesis without loss of androgens. | the intrinsic capacity of the immune system to elicit immune response selectively against late developing sperm proteins has been mobilized to intercept spermatogenesis. bacillus calmette-guérin given in appropriate doses intratesticularly is effective in bringing about this effect. in dogs and rhesus monkeys, the sperm count in the semen declined precipitously, and almost complete azospermia was attained in 4-6 weeks after immunization. the few sperm cells that were present were immotile. exami ... | 1979 | 118455 |
| the location and expression of idiotypic determinants in the immunoglobulin variable region--iii. expression of the protein 315 and 460 idiotypic determinants in mouse anti-dnp antibodies. | | 1979 | 93694 |
| [numerical analysis of relationships among mycobacterium tuberculosis, m. africanum, m. bovis, and m. microti (author's transl)]. | | 1979 | 119084 |
| nitrate reductase activity of mycobacterium tuberculosis and mycobacterium bovis in the presence of electron donors. | the activities of the nitrate reductase enzyme of mycobacterium tuberculosis, m. bovis, and of bcg were assayed with and without addition of electron donors. m. tuberculosis always reduced nitrate; m. bovis did so only in the presence of electron donors, and bcg did not show enzymatic activity. | 1978 | 97306 |
| the role of antibody and complement in phagocytosis by rabbit alveolar macrophages. | the relative importance of antibody and complement in the phagocytosis of staphylococcus aureus and pseudomonas aeruginosa, two common bacterial pathogens, by alveolar macrophages from rabbits was studied. normal rabbit serum was a satisfactory opsonin for the phagocytosis of s. aureus but not for p. aeruginosa. normal rabbit serum opsonized s. aureus by both the classic and the alternative complement pathways; loss of both pathways destroyed opsonic activity. the presence of complement was not ... | 1979 | 120385 |
| differentiation of bcg from other variants of mycobacterium tuberculosis isolated from clinical material. | bcg isolated from clinical material may be reliably differentiated from other variants within the species mycobacterium tuberculosis by means of a few simple cultural and biochemical tests and by bacteriophage typing. animal pathogenicity tests may therefore be avoided. | 1978 | 98878 |
| potency of ppd preparations derived from m. tuberculosis and m. bovis. | no significant difference was found between the biological activity of ppd-preparations derived from m. tuberculosis and m. bovis, although the latter was more potent. in potency tests performed in guinea-pigs, the use of difference mycobacteria as sensitizing agents had no influence on the result of the assay. we could not demonstrate a significantly greater potency of homologous versus heterologous ppd. evaluation of the 95 p.c. fiducial limits established in the course of variance analysis su ... | 1979 | 121875 |
| immunogenicity of an aerogenic bcg vaccine in t-cell-depleted and normal mice. | aerogenic infection of adult thymectomized, lethally irradiated, bone marrow-reconstituted (thxb) c57b1 times c3h f1 hybrid mice with 1 to 3,000 viable bcg montreal was followed by an extended period of logarithmic growth to a maximum population of 5 times 10-6 bacilli by day 35. the infection spread to the liver, spleen, and bone marrow with extensive multiplication in all test organs before the growth curves abruptly entered a stationary phase. up to 30% of the thxb mice eventually died as a r ... | 1975 | 123519 |
| [nomenclature of tuberculosis bacilli. the status of bcg. tuberculins and other mycobacterins]. | | 1978 | 100221 |
| rodent malaria: bcg-induced protection and immunosuppression. | one dose of 10(7) viable units of mycobacterium bovis, strain bcg, protected a significant number of swiss mice from a primary challenge with 10(4) thoracic sporozoites of plasmodium berghei. immunization with irradiated sporozoites induced greater protection than that observed in bcg-treated with bcg and surviving a primary sporozoite challenge were not protected from rechallenge, whereas mice immunized with irradiated sporozoites and surviving initial challenge of sporozoites were solidly immu ... | 1978 | 100553 |
| mycobacterial adjuvant and its carrier. | | 1975 | 125657 |
| enhancing effect of coenzyme, q10 on immunorestoration with mycobacterium bovis bcg in tumor-bearing mice. | effect of the additional treatment with coenzyme q10 on immunorestoration with mycobacterium bovis bcg in tumor-bearing mice was investigated. cell-mediated cytotoxicity in tumor-bearing mice against alloantigenic tumor cells was determined by 51cr release assay using spleen cells of c57bl/6n mice which had been inoculated subcutaneously with syngeneic melanoma-b16 and immunized intraperitoneally with alloantigenic mastocytoma p815-x2 cells. the cell-mediated cytotoxicity against mastocytoma p81 ... | 1978 | 101414 |
| chemical structure of the cell wall of mycobacterium tuberculosis var. bovis, strain bcg. | bcg cell walls contain approximately 30% free lipids like other mycobacterial cell walls. the insoluble skeleton of the cell wall is made up of two covalently linked polymers, a peptidoglycan and an arabinogalactan mycolate, with which are associated non peptidoglycan amino acids and a glucan. we present data on two structural features: 1. the "non peptidoglycan" amino acids; they form two kinds of compounds: peptide chains which can be solubilized by proteolytic enzymes and a trypsin-chymotryps ... | 1975 | 126548 |
| [catalases of mycobacteria as antigens. i. isolation, purification and characterization of catalases from different mycobacteria (author's transl)]. | preparations of catalase isolated from the strains m. tuberculosis, m. kansasii and m. bovis bcg are produced for testing their antigenic activity. after desintegration of the bacteria the highest activity remained in the precipitation with 50% saturated ammonium sulphate solution. the further purification of the catalase-fractions occurred with the aid of column chromatography on sephadex g 200 and deae-sephadex-a 50 after ultrafiltration. in this way the relative activity increased in m. tuber ... | 1978 | 102090 |
| [detection of pathogenic mycobacteria in the environment of the medical units and of the slaughter-house of an african town (author's transl)]. | the authors have made investigations about the presence of pathogen mycobacteria in puddles of rain water and in rill waters of sanitary formations and municipal slaughter-house of yaoundé. 19 strains of pathogen mycobacteria have been isolated from 84 water samples : 15 m. tuberculosis strains, especially present in the environment of sanitary formations, 4 m. bovis strains, especially present in the environment of the slaughterhouse. the third part of isolated m. tuberculosis strains belongs t ... | 1978 | 102900 |
| [interspecies circulation of mycobacteria]. | | 1975 | 129774 |
| immune mechanism in pasteurella multocida-infected mice. | various immunizing procedures were tested for their effect on the growth of pasteurella multocida in specific pathogen-free cd-1 mice. two injections with killed vaccine incorporated into freund complete adjuvant gave excellent protection against parenteral challenge, but less to control the growth of an aerogenic inoculum. protection with adjuvant-treated preparation was always superior to that seen with non-adjuvant-treated vaccines. a killed saline-suspended vaccine introduced aerogenically m ... | 1976 | 131783 |
| adjuvant and mitogenic properties of a supernatant fraction of sonically treated myobacterium bovis (bcg). | a water-soluble, oil-free supernatant fraction of sonically treated bcg (bcg-ss) was shown to be an immunological adjuvant and a mitogen. when bcg-ss and sheep erythrocytes (srbc) were injected intravenously into mice, the plaque-forming cell (pfc) response was 10 times greater than that induced by injection of srbc alone. circulating antibody responses to srbc and to bovine serum albumin were also enhanced by bcg-ss. the in vitro enhancement of pfc and circulating antibody responses did not req ... | 1976 | 133083 |
| organization of allergic granulomas and dependence on insoluble antigen. | | 1978 | 104033 |
| cell-mediated and humoral immune responses of cattle to brucella abortus, mycobacterium bovis, and tetanus toxoid: evaluation of immunization and assay techniques. | a concentration of 2.5 x 10(-5) m 2-mercaptoethanol (2-me) added to the medium in lymphocyte blastogenesis assays increased both the uptake of [3h]thymidine in unstimulated lymphocyte cultures and the probability of detecting antigen-sensitized cattle. the use of 2-me did not cause lymphocytes from unsensitized cattle to react positively in blastogenesis assays. a crude brucella lysate prepared from brucella abortus strain 19 was compared with a well-characterized brucella protein allergen prepa ... | 1978 | 104645 |
| cell-mediated and humoral immune responses of cattle to brucella abortus, mycobacterium bovis, and tetanus toxoid: immunization of the fetus. | | 1978 | 104646 |
| the effect of cortisone on the accumulation, activation, and necrosis of macrophages in tuberculous lesions. | rabbits were injected intramuscularly with cortisone acetate (2 mg/kg) on alternate days. six days after the first injection these rabbits and controls were injected intradermally in multiple sites with bcg (the vaccine strain of tubercle bacillus). periodically, over the next 2 months, the resulting lesions were measured and surgically biopsied, and the animals were tuberculin-tested. macrophage activation in the bcg lesions was evaluated histochemically by staining for beta-galactosidase activ ... | 1978 | 104930 |
| cross-reactions between mycobacteria. ii. crossed immunoelectrophoretic analysis of soluble antigens of bcg and comparison with other mycobacteria. | cross-reactions between mycobacterium bovis bcg and various other mycobacteria, nocardia asteroides, corynebacterium pyogenes and listeria monocytogenes were studied by incorporating antibodies against these bacteria in the intermediate gel of a crossed immunoelectrophoretic system with bcg antigen and anti-bcg antibodies. in the bcg reference system forty-four distinct antigenic components were recorded, of which thiryt-three cross-reacted with mycobacterium tuberculosis, twenty-five with m. av ... | 1979 | 106465 |