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comparison of the specificity of human and bovine tuberculin ppd for testing cattle. 2. south-eastern england.a tuberculin testing trial was carried out in eight counties of south-eastern england to compare the specificity for bovine tuberculosis of weybridge human ppd with that of rotterdam bovine ppd. the matching of these two tuberculins for potency in naturally infected cattle had already been established, the bovine ppd being approximately one-and-a-half times more potent than the human ppd per unit of weight. in 1110 cattle in 25 herds with histories of long-standing freedom from tuberculosis and ...197547751
comparison of the specificity of human and bovine tuberculin ppf for testing cattle. 3. national trial in great britain.a field trial on a country-wide basis was undertaken to compare the specificity for bovine tuberculosis of single and comparative tuberculin tests in cattle using either weybridge human or weybridge bovine ppd. the tests were made on 10,305 cattle in 179 herds distributed throughout all regions of england, scotland and wales. results showed that a comparative tuberculin test using avian ppd with either human or bovine ppd had a much higher efficiency than a single injection of mammalian tubercul ...197547752
specificity of antigenic fractions of tuberculin.purified protein derivative from mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 v/40 ma in ph 4.2 acetic acid-pyridine buffer. further purification of one of the fractions on sephadex g 25 column and by acid hydrolysis yielded antigen "ps" eliciting tuberculin reaction only in animals vaccinated with m. bovis bcg but not in those sensitized with m. avium and some fast-growing mycobacteria. the specificity of antigen "ps" was confirmed in vitro: the antigen induced ...197659518
evaluation of a dual-staining method for acid-fast bacilli.a dual-staining procedure for acid-fast bacilli was found to have poor correlation with the ziehl-neelsen and auramine-rhodamine staining techniques.197661205
common antigen of mycobacterium leprae, m. lepraemurium, m. avium, and m. fortuitum in comparative studies using two different types of antisera.no. 21 mycobacterial antigens of mycobacterium lepraemurium, m. avium, m. fortuitum, and m. leprae were compared in crossed immunoelectrophoresis using two different antibody sources, a serum pool from lepromatous leprosy patients (lsii) and a rabbit anti-m. smegmatis antiserum. m. lepraemurium, like m. avium, was found to contain the 21 a and 21 c determinants. m. fortuitum contained in addition a new type of determinant, 21 d.m. leprae antigen no. 21 carried the a as well as the b dertminants, ...197768004
endocarditis with acid-fast organism after porcine heart-valve replacement. 197881398
[experiments for the serological establishment of the species of mycobacteria inducing an immune restructuring of the macroorganism].serological investigations are carried out on hyperimmune rabbit and guinea pig sera with two antigens each of mycobacterium tuberculosis h37rv, mycobacterium bovinus, mycobacterium avium and mycobacterium kanzassi. one of the antigens is obtained through electrohydraulic destruction of delipidated mycobacteria, while the other constitutes cell walls, purified with ribonuclease, of non-delipidated mycobacterial cells, destroyed by the same method. the antigens, prepared by the electrohydraulic m ...197887060
differentiation of mycobacteria by "in vivo" and "in vitro" delayed hypersensitivity reactions.guinea pigs were sensitized with heat killed m. tuberculosis, m. kansasii, m. scrofulaceum, and m. avium-intracellulare. purified protein derivatives (ppds) prepared from each of these organisms were tested for delayed type hypersensitivity by lymphocyte transformation, macrophage migration, and mantoux type skin sensitivity tests. the homologous reactions were consistently more intense than heterologous reactions in all three procedures. the skin test procedure was more sensitive than either in ...197898814
[comparative study of virulence of mycobacterium xenopi and other mycobacteria in the rabbit (author's transl)]. 1978102134
[lymph node tuberculosis--today].the peripheral tuberculosis of the lymph nodes on the neck, in the axilla and on the groin is described in its present epidemiological situation, its pathogenesis, diagnosis and therapy. nowadays the tuberculosis of the lymph nodes most frequently occurs at older age and in women. in most cases the m. tuberculosis is the causative organism. the differential diagnosis must take into consideration many possibilities and sources of error--also for the following therapy and expert opinion--, in most ...1978104459
cross-reactions between mycobacteria. ii. crossed immunoelectrophoretic analysis of soluble antigens of bcg and comparison with other mycobacteria.cross-reactions between mycobacterium bovis bcg and various other mycobacteria, nocardia asteroides, corynebacterium pyogenes and listeria monocytogenes were studied by incorporating antibodies against these bacteria in the intermediate gel of a crossed immunoelectrophoretic system with bcg antigen and anti-bcg antibodies. in the bcg reference system forty-four distinct antigenic components were recorded, of which thiryt-three cross-reacted with mycobacterium tuberculosis, twenty-five with m. av ...1979106465
induction kinetics of delayed hypersensitivity towards mycobacterium tuberculosis and m. avium: analysis of cross-reactions elicited by ppd-tuberculin and ppd-b. 1978108772
deoxyribonucleic acid relatedness among species of slowly-growing mycobacteria.dna-dna hybridization is a reliable method for determining the phylogenetic relationship between bacterial strains the hybridization kinetics for dna from different slowly-growing mycobacteria were measured optically in a spectrophotometer. the results indicate that m. tuberculosis and m. bovis belong to one species. m. avium and m. intracellulare are two species, but some serotypes, now designated m. intracellulare, actually belong to m. avium.1979115225
mycobacterium avium serotype 4 infection in swine. 1979298934
comparison of techniques for measuring the local and systemic responses to tuberculin in cattle.thermography of tuberculin skin reactions in cattle sensitized with mycobacterium avium, mycobacterium bovis, or mycobacterium paratuberculosis was rapid and sensitive, but was not as specifically related to the homologous sensitization as was increased skin thickness at 48 and 72 hours. systemic temperature responses were more completely monitored by radiotelemetry of intraperitoneally located temperature transmitters than by rectal thermometry, but the temperatures indicated by the 2 methods w ...1977319714
tuberculin reactions in bulls and boars sensitized with atypical mycobacteria from sawdust. 1977333885
serological responses in experimental bovine tuberculosis.five two-year-old heifers were each inoculated intravenously with 0.02 mg m. bovis strain an5. clinical, haematological and microbiological observations were made during the course of the experiment and antibody levels were measured before and after infection by means of the indirect immunofluorescent antibody (ifa) and bentonite flocculation tests. all cattle developed m. bovis infection varying in severity from peracute tuberculous pneumonia resulting in death within 33 days to chronic progres ...1977336017
cell-mediated immunity in neonatal calves: delayed-type hypersensitivity and lymphocyte blastogenesis following immunization with a mycobacterial immunopotentiating glycolipid and tuberculoproteins of mycobacterium bovis. 1978347995
an enzyme-labeled antibody test for detecting antibodies in chickens infected with mycobacterium avium serotype 2.an enzyme-labeled antibody test was used for detecting antibodies in serums from chickens infected experimentally with mycobacterium avium serotype 2. positive ela reactions were observed in the serums of each of 8 chickens 2, 4, 6, and 8 weeks after infection; no reactions were observed in uninfected controls. tuberculin skin tests did not induce positive ela test reactions in uninfected chickens.1978348188
reliable technique of preparation of ultrathin sections of tissue infected with mycobacteria.a technique of preparation of ultrathin sections of liver tissue infected with mycobacteria, concretely of kupffer cells containing mycobacterium avium, was described. satisfactory preservation of the ultrastructure of both liver and mycobacteria was achieved by a modification of sampling, dehydration and vestopal-embedding procedures.1978359437
production of reference ppd tuberculins for veterinary use in the united states. 1978363720
experimental infection with mycobacterium avium, serotype 2, in pigs. 5. the immunizing effect of bcg vaccine against m. avium infection. 1978364966
bovine tuberculosis in the united states and puerto rico: a laboratory summary.mycobacteria were isolated from 14.3% of the tissues submitted to the national veterinary services laboratories over a 5-year period (july 1, 1972, to june 30, 1977). the isolates were identified by drug susceptibility, and biochemical and serologic tests. mycobacterium bovis isolated from tissues of cattle originating in 32 states and puerto rico accounted for 78% of the acid-fast isolations. of the mycobacterium bovis isolates, 4% were from tissues in which no microscopic tuberculous granuloma ...1979378044
lymphocyte transformation test in healthy contacts of patients with leprosy. i. influence of exposure to leprosy within a household.fifty-three household contacts of lepromatous patients, 37 household contacts of tuberculoid patients, and 91 control persons were examined with the lymphocyte transformation test (ltt) for their responses to whole and sonicated antigen preparations from m. leprae, to bcg, m. avium, m. gordonae, and phytohemagglutinin (pha). the study was carried out in the gurage area of ethiopia in 15 households with a leprosy patient and 15 matched control households. household contacts of lepromatous patient ...1979378871
[mycobacterial infections in nude mice (author's transl)]. 1979388019
avian immune response to mycobacterium avium strains of different virulence. 1979391011
laboratory diagnosis of bovine tuberculosis.lesions of suspected bovine tuberculosis were examined by culture, histopathology and auramine-o (ao) stained smears and the findings correlated with field aspects of the disease. of 642 lesions considered to be tuberculous, 62.0% yielded m. bovis and 4.5% other mycobacteria (om). m. bovis and om were recovered also from 0.6% and 3.6% respectively of 165 cattle which gave tuberculin reactions but had no visible lesions at slaughter. of 262 lesions in which a histopathological diagnosis other tha ...1979396918
[evolution and outcome of the tuberculous process produced by various mycobacterium tuberculosis in rabbits]. 1977402002
mycobacteria isolated from exotic animals.mycobacteria were isolated from 263 of 474 specimens submitted from captive exotic (nondomesticated) animals over a 5-year period. mycobacterium avium was isolated from 128 animals originating in 13 states and the district of columbia; serotype 1 accounted for 65 of the isolations. mycobacterium bovi was isolated from 74 animals in 7 zoos, 7 game parks, and 4 primate colonies in 1, states: mycobacterium tuberculosis was isolated from 29 animals originating 9 stats; and mycobacterium fortuitum, m ...1977406254
[types of mycobacteria and incidence of tuberculosis]. 1977406615
separation of the mixture of trehalose 6,6'-dimycolates comprising the mycobacterial glycolipid fraction, "p3". 1977409398
[isolation and identification of atypical mycobacteria (author's transl)]. 1977410050
[isolation of mycobacterium tuberculosis cultures from soil]. 1977411130
lymphocyte transformation: an aid in the diagnosis of paratuberculosis. 1977412443
lymphocyte subpopulations in spontaneous disease of rhesus macaques.the immunologic status of rhesus macaques (macaca mulatta) with naturally occurring disease was evaluated by determining the percentages of b and t lymphocytes and mitogen responsiveness of lymphocytes in peripheral blood and lymph nodes. the b lymphocytes were identified by the presence of cell surface immunoglobulin and receptors for complement. the t lymphocytes were identified by their ability to form spontaneous rosettes with sheep red blood cells. rhesus macaques with idiopathic or primary ...1977412446
studies on corynebacterial precipitinogens common to mycobacteria, nocardiae and rhodochrous.ten strains of corynebacterium were analyzed by means of the comparative immunodiffusion technique employing reference precipitation systems from strains of mycobacterium, nocardia and the rhodochrous taxon. the test strains had precipitinogens in common with the reference strains. two of the intergenerical cross-reacting precipitinogens revealed were labelled x and y. two other precipitinogens (alpha,beta), earlier found to be common for strains of mycobacteria, nocardiae, and rhodochrous, were ...1977412795
[use of paper indicators for the identification of mycobacteria]. 1977416434
in vitro lymphocyte transformation as a herd survey method for bovine paratuberculosis.the lymphocyte-transformation (lt) test was evaluated for its potential application as a field test for bovine paratuberculosis. using a whole blood technique, samples from 3 consecutive collection periods were subjected to 3 mycobacterial antigens and to phytohemagglutinin. the results obtained from lt were compared with conventional serologic and cultural methods. a positive lt response to johnin purified-protein derivative (ppd) or avian ppd (or both) was noted in 40% to 60% of the animals te ...1978417649
genetic transfers in mycobacteria. 1978417980
[atypical mycobacteria and their pathogenicity for agricultural animals]. 1978418560
[comparative electron microscopic study of mycobacteria]. 1979433135
[infections caused by the so-called atypical mycobacteria (author's transl)].a number of cases of patients in whom the so-called atypical mycobacteria were identified in our institute are presented briefly in this report. predisposing factors such as occupation and existing tissue damage, particularly in prolonged respiratory tract diseases, play a significant role in the establishment of the infection in the host organism. the bacteriological culturing, testing and typing of these organisms is an important adjunct to the clinical picture.1979437896
the sensitization of children by opportunist mycobacteria in lagos, nigeria.groups of school children aged 6-14 years were tested with ppds and one of the following five antigens simultaneously, namely ppda, ppdf, ppdg, ppdpl and ppdy, ppds was prepared from the human tubercle bacillus, and the others were prepared from m. avium, m. fortuitum, the 'gause' organisms, m. marinum and m. kansasii respectively. it was observed that there was some increase in induration size to ppda, ppdg, ppdl, ppds and ppdy with increase in age, while m. fortuitum gave a preponderance of sm ...1978418112
the extent and importance of sensitization by opportunist mycobacteria in lagos, nigeria.3505 school children aged 6 to 15 years were tested with ppds and at the same time one of the following tuberculins, namely, ppdg, ppdpl, ppda, ppdf and ppdy. the tuberculins were prepared from human tubercle bacillus, the gause organism, m. marinum, m. avium, m. fortuitum and m. kansasii respectively. ppdy, ppda and ppdg gave more reactions of greater than 2 mm induration than did ppds. furthermore, tests with ppdy, ppda, ppdg and ppdpl showed a significant proportion of children with reactions ...1977414397
infection of a dog with mycobacterium avium serotype ii. 1979442467
pyrolysis-gas-liquid chromatography of mycobacteria. 1977410231
[occurrence of m. avium and m. intracellulare in the organs and muscles of slaughterhouse pigs]. 1977406713
mycobacterium avium infection of the skin resembling lepromatous leprosy.a patient on systemic steroid therapy developed a cutaneous papule while histologically resembled lepromatous leprosy. cultures of this lesion grew mycobacterium avium. since there was no evidence of disseminated infection the lesion was excised and the patient continues to do well.1979475990
the use of polyvalent sera for the serotyping of mycobacteria within the mycobacterium avium--m. intracellulare--m. scrofulaceum complex. 1979479050
gas chromatographic characterization of porcine and human strains belonging to the mycobacterium avium-intracellulare complex.trifluoroacetylated whole-cell methanolysates of 23 strains designated as belonging to the mycobacterium avium-intracellulare complex by biochemical, growth chromogenicity and chicken pathogenicity tests, were analysed by gas chromatography. twenty of the strains were isolated from pigs and the remainder from human beings. serological typing showed that 13 of the porcine strains, but none of the human strains, belonged to m. avium. the remaining strains, except one which showed autoagglutination ...1979382739
[mycobacteria in arthropodes of different biotopes (author's transl)].many arthropodes are found in close contact with soil and other material contaminated by mycobacteria. in order to clear up their importance as potential carriers of germs of the mais complex (mycobacterium avium, intracellulare, scrofulaceum), we investigated 835 samples of arthropodes taken from different biotopes. following a treatment according to the cultural method developed by us (beerwerth, 1967), we isolated 606 strains of mycobacteria from 302 (36.0%) out of 835 samples. incubation at ...1979506541
[histological findings after application of kveim antigen in animals (author's transl)].the frequence of local granulomatous changes after injection of kveim antigen and previous sensibilisation (freund's adjuvant, bcg-vaccine, atypical mycobacteria, implantation of sarcoidotic lymph nodes, human-gamma-globulin of sarcoidotic patients) was investigated in 69 mice and 38 guinea pigs. granulomata with epitheloid and giant cells, resembling a "positive kveim reaction" were found in 26% of the mice and 18% of the guinea pigs. doubtful positive results were observed in 7% of the mice an ...1977349899
influence of pork consumption on human infection with mycobacterium avian-intracellulare.the hypothesis that eating pork from animals infected with organisms of the mycobacterium avium-intracellular complex is a significant cause of human mycobacteriosis was examined by skin testing students with purified protein derivative from m. intracellulare to determine their sensitivity to m. avium-intracellulare. no significant differences in sensitivity could be demonstrated between those individuals who had never eaten pork and those who ate pork routinely. it was concluded that sensitivit ...1979526017
occurrence and characterization of "avium-like" mycobacteria isolated from animals in sweden.431 cultures of "avium-like" mycobacteria (alm) were isolated from wild and domestic animals during 1974--76 at the national veterinary institute, stockholm. of these, 50 isolates from pigs were studied by growth-chromogenicity, pathogenicity, and biochemical tests. furthermore, thin-layer chromathography was performed, and on some isolates serotyping. all 50 isolates belonged to runyon's group iii and were pathogenic for chicken; none was capable of splitting oleic acid from tween 80. 47 gave t ...1979539419
standardisation of inocula for assessing the virulence of strains of avium-intracellulare mycobacteria in chickens. 1978340695
granulomatous synovitis: the role of atypical mycobacteria.clinical data on 25 patients with granulomatous synovitis and bursitis observed from 1970 through 1977 are reviewed. the lesions occurred about the extremities, the wrists and hands being involved most often. with three exceptions, the patients had no significant underlying disease. the lesions were chronic and often followed minor trauma. many patients had had prior surgery, steriod injections, or both. at the time of surgery for the synovitis, the gross appearance proved to be relatively chara ...1979542760
skin testing, fecal culture, and lymphocyte immunostimulation in cattle inoculated with mycobacterium paratuberculosis.fourteen calves at 21 days of age were experimentally inoculated with 100 mg (wet weight) of mycobacterium paratuberculosis. three calves were inoculated orally, 4 intravenously, and 7 subcutaneously. lymphocyte immunostimulation, fecal culture, and intradermal tuberculin skin testing were done between 112 to 150 days following exposure. lymphocyte immunostimulation test results, conducted at 112 days after inoculation, showed all animals positive to mycobacterium avium purified protein derivati ...1977339793
[antigenic and allergenic properties of various atypical mycobacteria and mycobacterium avium. ii. skin reactions to homologous and heterologous ppd preparations in artificially sensitized guinea pigs].albino guinea-pigs were artificially sensitized to m. kansasi, scotochromogenic bacilli of nicolas strain, m. fortuitum and m. avium and after 4 to 10 weeks 4,20 and 100 homological and heterologous units of ppd preparations were injected intradermally. it was shown that the studied bacilli had sensitizing properties too and ppd preparations obtained from them differ in biological activity and specificity. sensitine made from m. kansasi was characterized by the greatest sensitizing strength. bac ...1979554984
[atypical mycobacteria]. 1977337073
[the behaviour of mycobacterium intracellulare serotyp davis and mycobacterium avium in the head region of sphagnum moss vegetation after experimental inoculation (author's transl)].after the inoculation in the head region of sphagnum moss vegetation (sph. magellancium-erica association and sph. apiculatum-eriophorum angustifolium ass.) reach the mycobacteria the hyaline cells of sphagnum (fig. 1). the count of m. intracellulare serotyp davis decreases rapidly already in three days after the inoculation of sph. magellanicum ass. and this trend in the course of incubation is being continued. in 27 days following the inoculation only 2 of 7 specimens contained a small amount ...1978566009
method for evaluation of mycobacterium bovis purified protein derivative tuberculin in experimentally infected cattle.a method for the evaluation of mycobacterium bovis purified protein derivative (ppd) tuberculin in experimentally infected cattle is presented. the development of skin test responses in m bovis-infected cattle was determined for international standard ppd-s, m bovis ppd-2, and m bovis ppd-5 at 24, 48, and 72 hours. significantly larger reactions (dermal thickness) were observed at 48 and 72 hours than at 24 hours (p = 0.001). statistically significant differences were not detected in the respons ...1977329720
specific detection of mycobacteria by the immunofluorescent technique.using an indirect immunofluorescent technique, m. bovis var. bcg can be differentiated from m. avium in pure culture. the test involves using an unlabeled antiserum prepared against b-24, a type specific antigen of m. bovis var. bcg. in the same system m. bovis could be differentiated from other pathogenic my cobacteria in tissue sections of naturally and experimentally infected animals.1977328424
elevated adjuvanticity of non-acid-fast mycobacteria.administration to guinea pigs of non-acid-fast (naf) mycobacterium avium resulted in a sevenfold greater circulating antibody response than that for the animals receiving ovalbumin in freund incomplete adjuvant alone. the ability of naf m. avium on induction of delayed hypersensitivity was comparable or somewhat stronger than that of normal m. avium. the in vivo enhancement of circulating antibody response by naf m. avium associated with a marked stimulating effect of it on proliferation of b ce ...1979315375
metchnikoff-schaumann bodies in experimental yersin's type tuberculosis induced with mycobacterium avium in hamster.structure and ultrastructure of metchnikoff-schaumann bodies occurring in macrophages, epithelioid cells and multinucleated giant cells in tuberculous granuloma were investigated. their similitude with other reported intracellular calcareous bodies occurring in malakoplakia, infectious orchitis (michaelis-guttman' bodies or calcosphaerites), in beryllium granulomas (conchoid bodies) and sarcoidosis (schaumann bodies) is discussed.1979225661
iron-chelating compound from mycobacterium avium.a iron-chelating monohydroxamate was isolated from cultures of mycobacterium avium grown on an iron-limiting medium. the hydroxyamate metabolite was characterized by chemical degradation and spectral measurements as l-alpha-asparaginyl-l-alpha-(n-hydroxy)-asparagine.1976185194
the isolation of mycobacteria from the brown hare (lepus europaeus). 1977608080
[epidemiological and clinical importance of m. avium among the inhabitants of rostov province]. 1976134365
sensitivity to tuberculin and to a nonmammalian sensitin in mandalay district, burma.delayed hypersensitivity that can be demonstrated with either a strong dose of tuberculin or a conventional dose of a sensitin prepared from certain nonmammalian mycobacteria (mycobacteria of runyon groups ii and iii, e.g., mycobacterium avium), is known to be highly prevalent in most tropical and many subtropical areas and rare in many temperate zones. whether such sensitivity interacts significantly with tuberculosis or with leprosy is not known.a study of reactions to tuberculin (ppd-s) and t ...1975129303
[relationships between "mycobacterium simiae" and the "m. avium-intracellulare-scrofulaceum" complex (author's transl)].the 24 strains of mycobacterium simiae described in this report were isolated from 12 black africans, 6 from white europeans, 5 from primates and 1 from a leprosy infected armadillo. these strains form 3 groups having the similar morphologic and cultural properties as m. intracellulare. two groups were similar with respect to pigmentation, urease activity and niacin production but differed serologically, the second group being of m. intracellulare serotype 18. the third group was less homogenous ...1979120121
[relationship among mycobacterium avium, m. intracellulare, m. scrofulaceum, and m. gordonae observed by thin-layer chromatography of lipids after uptake of 35s-methionine (author's transl)]. 1978633705
[successful chemotherapy of avian mycobacteriosis with isoprodian (author's transl)]. 1978652713
tuberculin hypersensitivity following bcg vaccination in brisbane school children.seven hundred and fifty brisbane school children were tuberculin tested, in paired groups, with purified protein derivatives of mycobacterium tuberculosis, bovis, bcg and avium. reaction to avian ppd were stronger than to any of the others used. this finding may be of some importance in interpreting the variation in protection afforded by bcg vaccination.1979112744
demonstration of tuberculostearic acid in sputum from patients with pulmonary tuberculosis by selected ion monitoring.selected ion monitoring was used to detect tuberculostearic acid (10-methyloctadecanoic acid) in sputum from patients with pulmonary tuberculosis. the specimens were autoclaved, lyophilized, extracted, and methanolysed before being subjected to thin-layer chromatography and injected into the gas chromatograph/mass spectrometer. tuberculostearic acid could be detected in five of six tuberculous sputum specimens containing acid-fast rods detectable by light microscopy of ziehl-neelsen stained smea ...1979109465
heat inactivation of mycobacterium avium-mycobacterium intracellulare complex organisms in aqueous suspension.isolants from swine and from humans representing serotypes 1, 2, 4, 8, and 10 of the mycobacterium avium-mycobacterium intracellulare complex were compared for heat tolerance in aqueous suspension. the most heat-resistant isolant found was a serovar 10 isolated from a human. this isolant was examined further to determine the rate of kill at various temperatures and ph's, the effect of meat protein and fat, and the effect of nitrite. kill rates were not significant at 60 degrees c or below. decim ...197944444
[secondary infection of mycobacterium avium-intracellulare complex to open-negative cavities of lung tuberculosis (author's transl)]. 1979439568
structural studies on the type-specific antigens and lipids of the mycobacterium avium. mycobacterium intracellulare. mycobacterium scrofulaceum serocomplex. mycobacterium intracellulare serotype 9. 1979438184
comparison of four culture media for isolation of mycobacterium avium complex from porcine tissues.the efficiency of four culture media was compared for the isolation of mycobacterium avium complex from 197 procine tissues. in 82 tissues with microscopic granulomas and acid-fast bacilli, a significantly greater number of isolates were obtained on middlebrook 7h10 medium with sodium pyruvate than on stonebrink medium, herrold egg yolk agar medium, or lowenstein-jensen medium (p=0.01). in 46 tissues in which no microscopic granulomas or acid-fast bacilli were observed, a significantly greater n ...1979429541
a demographic study of disease due to mycobacterium kansasii or m intracellulare-avium in texas.the number of patients reported th have disease due to mycobacterium kansasii or m intracellulare-avium has increased in texas from january 1967 to december 1976, in contrast to a decrease in tuberculosis. presented is an analysis of 1,409 patients infected with m kansasii and 706 patients infected with m intracellulare-avium. the former group clustered in urban areas with more than twice the incidence compared to nonurban areas (p less than 0.001). the latter group was more diffusely distribute ...1979421546
pulmonary mycobacterial infections due to mycobacterium intracellulare-avium complex. clinical features and course in 100 consecutive cases.one hundred consecutive cases of pulmonary infection due to mycobacterium intracellulare-avium seen during a 3 1/2-year period qualified for review on the basis of a compatible chest x-ray film, repeated isolations from cultures of sputum, and follow-up of three to eight years. infections with m intracellulare-avium represented 27 percent of all mycobacterial infections seen during this period, including those due to m tuberculosis. the cases of disease due to m intracellulare-avium were predomi ...1979421545
mycobacterium avium abortion in a sow.a 2-year-old sow aborted her entire near-term litter of 11. gross and histologic examination of a fetus suggested a tuberculous infection, and a yellow-pigmented mycobacterium avium serotype 1 was subsequently isolated from the fetal tissue. efforts to rebreed the sow were unsuccessful. she was anergic to skin tests with purified protein derivative of m. avium on two occasions but had m. avium specific in vitro lymphocyte immunostimulation. gross granulomatous lesions were found in the liver, k ...1979442461
lesions of swine lymph nodes as a diagnostic test to determine mycobacterial infection.decisions as to the disposition of swine carcasses with lesions attributable to a mycobacterial infection are based upon a lesion criterion which is used as a diagnostic test by federal meat inspectors. using this criterion, a federal meat inspector classified 58 of 100 pigs as "passed for cooking" and the other 42 pigs passed. of the 58 pigs passed for cooking and the 42 pigs passed, mycobacterial isolations were made from the lymph nodes of 33 and 15 of the animals, respectively. the lesion cr ...1979453840
experimental challenge of red deer with mycobacterium avium. 1978685094
"pig-compost" as a source of mycobacterium avium infection in swine. 1978696079
isolation of plasmids from mycobacteria.a procedure for preparing sodium dodecyl sulfate-salt-cleared lysates of mycobacteria was developed. standard techniques were employed to demonstrate covalently closed circular deoxyribonucleic acid in lysates of nine strains of mycobacterium avium-intracellulare.1979468387
[experimental findings after application of kveim antigen. ii. sensibilization of mice and guinea pigs by mycobacterium avium (serotype i) (author's transl)].mice and guinea pigs were sensibilisized by intraperitoneal injection of mycobacterium avium (serotype i). three and five weeks later (guinea pigs) and four weeks later (mice) the animals received kveim antigen into the footpads (guinea pigs) and into the perianal fat tissue (mice). three and five weeks later (guinea pigs) and four weeks later (mice) the exstirpated material was investigated by light microscopy and enzyme histochemistry. guinea pigs showed three and five weeks after injection of ...1979494694
enzyme-linked immunosorbent assay for detecting antibodies in swine infected with mycobacterium avium.enzyme-linked immunosorbent assay (elisa) was used for detecting the antibodies in sera from swine experimentally infected with mycobacterium avium. positive elisa reactions were observed in the sera of each of six swine at postinoculation weeks 2, 4, 6, 8, and 10; no reaction was observed in noninoculated controls. the elisa reactions were observed in each of two swine at 4, 6, 8, 10 weeks following exposure to m avium-infected swine. mycobacterium avium-purified protein derivative and killed c ...1979525914
bone lesions in three tree kangaroos. 1978738924
actinomycotic lesions in addition to tuberculous infections in slaughtered pigs. 1979541457
[experimental findings after application of kveim antigen. iii. sensitization of mice with living and killed strains of mycobacterium avium (author's transl)].mice were infected with mycobacterium avium (serotype i and serotype ii). the application of kveim antigen or two kinds of lipid fractions of mycobacterium avium (serotype i or ii) is followed in all cases by typical tissue reactions resembling positive kveim tests. control animals which only received kveim antigen or lipid fractions of mycobacterium i showed in all cases negative kveim reactions. sensibilization of mice with killed kinds of mycobacterium avium i or ii leads after application of ...1979552201
[antigenic and allergenic properties of various atypical mycobacteria and mycobacterium avium. i. immunoelectrophoretic analysis of antigens in water extracts].4 strains of acid-resistant bacilli were examined: m. kansasi, scotochromogenic bacilli of nicolas strain, m. fortuitum and m. avium d4. water extracts prepared from these strains according to whiteside and baker method were used as antigens in the immunoelectrophoresis reaction and were also used to obtain specific serums on rabbits which were hyperimmunized according to tuboly's method. in the immunoelectrophoresis reaction water extracts were examined against nonsaturated serums, as well as c ...1979554988
the specificity and sensitivity of the fluorescent antibody test in cattle experimentally infected with mycobacterium avium and mycobacterium johnei.in five calves experimentally infected with mycobacterium avium and in 10 with m johnei it was shown that the serological response by the fluorescent antibody (fa) test was specific. the serological response in the m avium infected calves was transitory and lasted up to five months. the first evidence of a serological response with the fa test in the m johnei dosed calves occurred at four months, and all calves had reacted by nine months after dosing. at the 23 tests carried out between four and ...1976769123
experimental mycobacterial infection in congenitally athymic "nude" mice. 1976772204
[present status of classification of "atypical mycobacteria" (author's transl)]. 1976781372
lymphocyte stimulation and leucocyte migration inhibition as diagnostic tools for the detection of mycobacterium avium infection in cattle. 1976782216
enhancement of humoral immune responses. i. potentiating influence of purified protein derivative on the invitro immune response of spleen cells sensitized to mycobacterium bovis and mycobacterium avium.addition of purified protein derivate (ppd) to suspension cultures of spleen cells from swine sensitized to mycobacterium bovis and mycobacterium avium resulted in marked enhancement of antisheep erythrocyte plaque-forming cells after stimulation with sheep erythrocytes. the enhancing effect appeared early in the response and was specific for the sensitizing antigen. the enhancing effect was dependent upon the presence of both sheep erythrocytes and ppd in the culture system. ppd had no effect i ...1976797671
lesions and tuberculin sensitivity in calves inoculated with group iii mycobacterial isolates from swine, pen soil, and cattle feed.fifteen calves, 7 to 11 months of age, were inoculated intradermally with group iii mycobacteria ---6 isolates from swine, 1 isolate from cattle feed, and 2 isolates from soil of swine farrowing pens. calves were tuberculin tested at 50 days and killed approximately 60 days after inoculation. only 1 of the cultures, that of porcine origin (93c-0), produced any lesions. there were a caseo-calcareous granuloma 3mm in diameter in the regional lymph node (left prescapular) and a granuloma at the ski ...1975806238
comparison of lymphocyte stimulation and tuberculin skin reactivity in mycobacterium bovis-infected macaca mulatta.lymphocyte immunostimulation tests were conducted on mycobacterium bovis-infected and tuberculin-negative rhesus macaques (macaca mulatta), using purified protein derivative (ppd) tuberculins prepared from strains of mycobacterium tuberculosis and mycobacterium avium. the stimulation of lymphocytes with m tuberculosis ppd was significantly greater for the m bovis-infected macaques than for the noninfected controls (p smaller than 0.05). the response of lymphocytes from m bovis-infected macaques ...1975806239
distribution of serotypes of the mycobacterium avium-intracellulare-scrofulaceum complex in georgia.cultures of the mycobacterium avium-intracellulare-scrofulaceum (mais) complex, isolated from patients who lived throughout the state of georgia, were studied using the serotyping scheme of schaefer. one hundred four (77 per cent) of the 135 isolates tested could be classified into 16 serotypes. the rest were not typable. fifty-eight per cent of all the typable strains were serotypes 1, 14, 16, and 42. results of the serotyping were recorded, mapped, and analyzed by county of residence. most fre ...1979572193
heat inactivation of mycobacterium avium-mycobacterium intracellulare complex organisms in meat products.wieners and sausages were prepared which contained the most heat-tolerant representative of the mycobacterium avium-mycobacterium intracellulare complex we were able to obtain. they also were prepared with infected tissues obtained from tuberculous swine. processing conditions were as varied as possible. neither incorporation of sodium nitrite in the emulsion nor presence of smoke during processing altered the heat susceptibility of the organisms. substantial killing of the organisms occurred as ...1979575610
the species distribution of three concanavalin a-purified mycobacterial polysaccharides.culture filtrates prepared from 12 species of mycobacteria were examined for the presence of 3 mycobacterial polysaccharides previously purified by concanavalin a affinity chromatography. four commercial old tuberculins, tuberculin tine test units, and purified protein derivative were also studied. all 3 polysaccharides were demonstrated in all of the filtrates and each of the old tuberculins and purified protein derivative. the amount of concanavalin a-nonreactive polysaccharide was measured in ...1975808990
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