| structure determination of two new amino acid-containing derivatives of adenosine from trna of thermophilic bacteria and archaea. | two new nucleosides have been identified in unfractionated transfer rna of two thermophilic bacteria, thermodesulfobacterium commune, and thermotoga maritima, six hyperthermophilic archaea, including pyrobaculum islandicum, pyrococcus furiosus and thermococcus sp. and two mesophilic archaea, methanococcus vannielii and methanolobus tindarius. structures were determined primarily by mass spectrometry, as 3-hydroxy-n-[[(9-beta-d-ribofuranosyl-9h-purin-6- yl)amino]carbonyl]norvaline, (hn6a), struct ... | 1992 | 1280806 |
| partial purification and characterization of the first hydrogenase isolated from a thermophilic sulfate-reducing bacterium. | a soluble [nife] hydrogenase has been partially purified from the obligate thermophilic sulfate-reducing bacterium thermodesulfobacterium mobile. a 17% purification yield was obtained after four chromatographic steps and the hydrogenase presents a purity index (a398 nm/a277 nm) equal to 0.21. this protein appears to be 75% pure on sds-gel electrophoresis showing two major bands of molecular mass around 55 and 15 kda. this hydrogenase contains 0.6-0.7 nickel atom and 7-8 iron atoms per mole of en ... | 1992 | 1317168 |
| purification and characterization of bisulfite reductase (desulfofuscidin) from desulfovibrio thermophilus and its complexes with exogenous ligands. | a dissimilatory bisulfite reductase has been purified from a thermophilic sulfate-reducing bacterium desulfovibrio thermophilus (dsm 1276) and studied by epr and optical spectroscopic techniques. the visible spectrum of the purified bisulfite reductase exhibits absorption maxima at 578.5, 392.5 and 281 nm with a weak band around 700 nm. photoreduction of the native enzyme causes a decrease in absorption at 578.5 nm and a concomitant increase in absorption at 607 nm. when reduced, the enzyme reac ... | 1990 | 2165817 |
| characterization of cytochrome c3 from the thermophilic sulfate reducer thermodesulfobacterium commune. | a c3 type cytochrome has been purified from the thermophilic, non-spore-forming, sulfate-reducing bacterium thermodesulfobacterium commune. the purified protein was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gel filtration, and isoelectric focusing. a pi of 6.83 was observed. the molecular weight of the cytochrome was estimated to be ca. 13,000 from both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the hemoprotein exhibite ... | 1984 | 6090384 |
| physiological function of hydrogen metabolism during growth of sulfidogenic bacteria on organic substrates. | desulfovibrio vulgaris madison and thermodesulfobacterium commune contained functionally distinct hydrogenase activities, one which exchanged 3h2 into 3h2o and was inhibited by carbon monoxide and a second activity which produced h2 in the presence of co. cell suspensions of d. vulgaris used either lactate, pyruvate, or co as the electron donor for h2 production in the absence of sulfate. both sulfidogenic species produced and consumed hydrogen as a trace gas during growth on lactate or pyruvate ... | 1984 | 6480553 |
| characterization of a new type of dissimilatory sulfite reductase present in thermodesulfobacterium commune. | a new type of dissimilatory bisulfite reductase, desulfofuscidin, was isolated from the nonsporeforming thermophilic sulfate-reducing microorganism thermodesulfobacterium commune. the molecular weight of the enzyme was estimated at 167,000 by sedimentation equilibrium, and the protein was pure by both disc electrophoresis and ultracentrifugation. the bisulfite reductase was a tetramer and had two types of subunits with an alpha(2)beta(2) structure and an individual molecular weight of 47,000. th ... | 1983 | 6826522 |
| novel division level bacterial diversity in a yellowstone hot spring. | a culture-independent molecular phylogenetic survey was carried out for the bacterial community in obsidian pool (op), a yellowstone national park hot spring previously shown to contain remarkable archaeal diversity (s. m. barns, r. e. fundyga, m. w. jeffries, and n. r. page, proc. natl. acad. sci. usa 91:1609-1613, 1994). small-subunit rrna genes (rdna) were amplified directly from op sediment dna by pcr with universally conserved or bacteria-specific rdna primers and cloned. unique rdna types ... | 1998 | 9440526 |
| kinetics of sulfate and hydrogen uptake by the thermophilic sulfate-reducing bacteria thermodesulfobacterium sp. strain jsp and thermodesulfovibrio sp. strain r1ha3 | half-saturation constants (km), maximum uptake rates (vmax), and threshold concentrations for sulfate and hydrogen were determined for two thermophilic sulfate-reducing bacteria (srb) in an incubation system without headspace. km values determined for the thermophilic srb were similar to the constants described for mesophilic srb isolated from environments with low sulfate concentrations. | 1999 | 10049897 |
| thermodesulfobacterium hveragerdense sp. nov., and thermodesulfovibrio islandicus sp. nov., two thermophilic sulfate reducing bacteria isolated from a icelandic hot spring. | two thermophilic non-sporeforming sulfate-reducing bacteria (srb) were isolated from microbial mats collected from an icelandic hot spring. strain jsp was a gram negative rod, with an average cell size of 2.8 x 0.5 microm. no flagella were found. growth occurred between 55 and 74 degrees c with an optimum between 70 and 74 degrees c at ph 7.0. the g+c content was 40 mol%. strain r1ha3 was a gram negative vibrio-shaped rod with an average cell size of 1.7 x 0.4 microm. motility was observed media ... | 1999 | 10794144 |
| characterization of a new thermophilic sulfate-reducing bacterium thermodesulfovibrio yellowstonii, gen. nov. and sp. nov.: its phylogenetic relationship to thermodesulfobacterium commune and their origins deep within the bacterial domain. | a thermophilic sulfate-reducing vibrio isolated from thermal vent water in yellowstone lake, wyoming, usa is described. the gram-negative, curved rod-shaped cells averaged 0.3 micrometer wide and 1.5 micrometers long. they were motile by means of a single polar flagellum. growth was observed between 40 degrees and 70 degrees c with optimal growth at 65 degrees c. cultures remained viable for one year at 27 degrees c although spore-formation was not observed. sulfate, thiosulfate and sulfite were ... | 1994 | 11541228 |
| multiple lateral transfers of dissimilatory sulfite reductase genes between major lineages of sulfate-reducing prokaryotes. | a large fragment of the dissimilatory sulfite reductase genes (dsrab) was pcr amplified and fully sequenced from 30 reference strains representing all recognized lineages of sulfate-reducing bacteria. in addition, the sequence of the dsrab gene homologs of the sulfite reducer desulfitobacterium dehalogenans was determined. in contrast to previous reports, comparative analysis of all available dsrab sequences produced a tree topology partially inconsistent with the corresponding 16s rrna phylogen ... | 2001 | 11567003 |
| thermodesulfobacterium hydrogeniphilum sp. nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent at guaymas basin, and emendation of the genus thermodesulfobacterium. | a thermophilic, non-spore-forming, marine, sulfate-reducing bacterium, strain sl6t, was isolated from deep-sea hydrothermal sulfides collected at guaymas basin. the gram-negative-staining cells occurred singly or in pairs as small, highly motile rods. the temperature range for growth was 50-80 degrees c with an optimum at 75 degrees c. the ph range for growth at 70 degrees c was 6.3-6.8, with an optimum at 6.5. the nacl concentration range for growth was 5-55 g l(-1), with an optimum at 30 g l(- ... | 2002 | 12054236 |
| valid publication of the genus name thermodesulfobacterium and the species names thermodesulfobacterium commune (zeikus et al. 1983) and thermodesulfobacterium thermophilum (ex desulfovibrio thermophilus rozanova and khudyakova 1974). opinion 71. | the judicial commission of the international committee on systematics of prokaryotes decided that the date of valid publication of the genus name thermodesulfobacterium and of the species names thermodesulfobacterium commune and thermodesulfobacterium thermophilum is 1995. thermodesulfobacterium mobile rozanova and pivovarova 1988 is an illegitimate, later synonym of thermophilum. | 2003 | 12807221 |
| thermodesulfatator indicus gen. nov., sp. nov., a novel thermophilic chemolithoautotrophic sulfate-reducing bacterium isolated from the central indian ridge. | a thermophilic, marine, anaerobic, chemolithoautotrophic, sulfate-reducing bacterium, strain cir29812t, was isolated from a deep-sea hydrothermal vent site at the kairei vent field on the central indian ridge. cells were gram-negative motile rods that did not form spores. the temperature range for growth was 55-80 degrees c, with an optimum at 70 degrees c. the nacl concentration range for growth was 10-35 g l(-1), with an optimum at 25 g l(-1). the ph range for growth was 6-6.7, with an optimum ... | 2004 | 14742485 |
| polyamine analysis for chemotaxonomy of thermophilic eubacteria: polyamine distribution profiles within the orders aquificales, thermotogales, thermodesulfobacteriales, thermales, thermoanaerobacteriales, clostridiales and bacillales. | cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by hplc and gc analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. spermidine and a quaternary branched penta-amine, n4-bis(aminopropyl)norspermidine, were the major polyamine in thermocrinis, hydrogenobacter, hydrogenobaculum, aquifex, persephonella, sulfurihydrogenibium, hydrogenothermus, balnearium and thermovibrio, located in the order aquificales. thermo ... | 2004 | 15747232 |
| immunomagnetically captured thermophilic sulfate-reducing bacteria from north sea oil field waters. | immunomagnetic beads (imb) were used to recover thermophilic sulfate-reducing bacteria from oil field waters from oil production platforms in the norwegian sector of the north sea. imb coated with polyclonal antibodies against whole-cell antigens of the thermophilic thermodesulfobacterium mobile captured strains gfa1, gfa2, and gfa3. gfa1 was serologically and morphologically identical to t. mobile. gfa2 and gfa3 were spore forming and similar to the desulfotomaculum strains t90a and t93b previo ... | 1992 | 16348693 |
| microbial evolution of sulphate reduction when lateral gene transfer is geographically restricted. | lateral gene transfer (lgt) is an important mechanism by which microorganisms acquire new functions. this process has been suggested central to prokaryotic evolution in various environments. however, the influence of geographical constraints on the evolution of laterally acquired genes in microbial metabolic evolution is not yet well understood. in this study, the influence of geographical isolation on the evolution of laterally acquired dissimilatory sulphite reductase (dsr) gene sequences in t ... | 2010 | 20802057 |
| microbial diversity with dominance of 16s rrna gene sequences with high gc contents at 74 and 98 °c subsurface crude oil deposits in japan. | we prepared dna from the production waters of oil deposits and wellheads of the high- and hypertemperature japanese oil wells #ar39 (depth, 1230 m; temperature, 74 °c; pressure, 2.92 mpa) and #sr123 (depth, 1687 m; temperature, 98 °c; pressure, 11.3 mpa) to detect indigenous bacterial and archaeal microorganisms. we used pcr to amplify the 16s rrna genes of microbial communities and characterized them based on their sequences. a few species of microorganisms with high gc contents were detected i ... | 2011 | 21223337 |
| sulfur-metabolizing bacterial populations in microbial mats of the nakabusa hot spring, japan. | at the nakabusa hot spring, japan, dense olive-green microbial mats develop in regions where the slightly alkaline, sulfidic effluent has cooled to 65°c. the microbial community of such mats was analyzed by focusing on the diversity, as well as the in situ distribution and function of bacteria involved in sulfur cycling. analyses of 16s rrna and functional genes (apra, pufm) suggested the importance of three thermophilic bacterial groups: aerobic chemolithotrophic sulfide-oxidizing species of th ... | 2011 | 21353426 |
| phylogenetic analysis reveals multiple lateral transfers of adenosine-5'-phosphosulfate reductase genes among sulfate-reducing microorganisms. | lateral gene transfer affects the evolutionary path of key genes involved in ancient metabolic traits, such as sulfate respiration, even more than previously expected. in this study, the phylogeny of the adenosine-5'-phosphosulfate (aps) reductase was analyzed. aps reductase is a key enzyme in sulfate respiration present in all sulfate-respiring prokaryotes. a newly developed pcr assay was used to amplify and sequence a fragment ( approximately 900 bp) of the aps reductase gene, apsa, from a tax ... | 2002 | 11741869 |