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phylogenetic analysis of the coryneform bacteria by 5s rrna sequences.nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ...19873106318
analysis of cellular fatty acids by gas chromatography as a tool in the identification of medically important coryneform bacteria.the fatty acid methyl esters of nineteen unidentified pathogenic coryneform bacteria were analysed by gas-liquid chromatography and the resulting profiles were compared with those of type or reference strains of possibly related species, namely caseobacter polymorphus, corynebacterium bovis, c. diphtheriae, c. xerosis and rhodococcus equi. all of the strains had distinct fatty acid profiles but most of them conformed to a general pattern, with high levels of octadecanoic acids and only trace amo ...19853924876
menaquinone composition of mycolic acid-containing actinomycetes and some sporoactinomycetes.the menaquinones of 141 actinomycetes representing the genera caseobacter, mycobacterium, nocardia, rhodococcus and some related taxa lacking mycolic acids were examined by mass spectrometry. the mycolic acid-containing strains were assigned to four groups on the basis of the predominant isoprenologue detected: rhodococcus coprophilus, r. equi, r. erythropolis, r. globerulus, r. rhodnii, r. rhodochrous and r. ruber contained dihydrogenated menaquinones with eight isoprene units; nocardia asteroi ...19853980298
a survey of the structures of mycolic acids in corynebacterium and related taxa.acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. the mycolic acids of corynebacterium sensu stricto strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of c22 to c36. two species, corynebacterium bovis and 'corynebacterium mycetoides', were particularly distinctive; the former had exceptionally low molecular weight mycolic a ...19827086391
identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses.coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ...19979039559
intraspecific variation of unusual phospholipids from corynebacterium spp. containing a novel fatty acid.the novel fatty acid trans-9-methyl-10-octadecenoic acid was isolated from the coryneform bacterial strain lmg 3820 (previously misidentified as arthrobacter globiformis) and identified by spectroscopic methods and chemical derivatization. this fatty acid is attached to the unusual lipid acyl phosphatidylglycerol. five different species of this lipid type were identified; their structures were elucidated by tandem mass spectrometry and are reported here for the first time. additionally, we ident ...19989721308
purification and characterization of an extracellular proline iminopeptidase from corynebacterium variabilis ncdo 2101.to screen the extracellular proteolytic and lipolytic activities of corynebacterium variabilis ncdo 2101 and to purify and characterize a proline iminopeptidase enzyme in order to investigate the role of the major component of the smear of bacterial surface-ripened cheeses.200111298241
corynebacterium mooreparkense sp. nov. and corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese.ten isolates each of two different bacterial species isolated from the surface of a smear-ripened cheese were found to exhibit many characteristics of the genus corynebacterium. the isolates were gram-positive, catalase-positive, non-spore-forming rods that did not undergo a rod/coccus transformation when grown on complex media. chemotaxonomic investigation revealed that the strains belonged unambiguously to the genus corynebacterium. their cell walls contained arabinose, galactose and short-cha ...200111411705
biodiversity of the bacterial flora on the surface of a smear cheese.the bacteria on the surface of a farmhouse smear-ripened cheese at four stages of ripening (4, 16, 23, and 37 days) from inoculated (i.e., deliberately inoculated with brevibacterium linens bl2) and noninoculated (not deliberately inoculated with b. linens bl2) cheese were investigated. the results show that, contrary to accepted belief, b. linens is not a significant member of the surface flora of smear cheese and no microbial succession of species occurred during the ripening of the cheeses. o ...200211823224
a novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface bacillus licheniformis strain 603.a new bacillus licheniformis strain, 603, isolated from a mixture of drilling fluid and subsurface thermal water, has been found to produce a cyclic lipopeptide which is released into cultural medium as well as present in cells as the major lipid constituent (57% of the total cell lipids extractable with 2:1 chloroform-methanol). the quantitative ratio of the extracellular and intracellular lipopeptide has been estimated as 23:10. the metabolite represents a heptapeptide, l-asp-->l-leu-->l-leu-- ...200314643798
corynebacterium mooreparkense, a later heterotypic synonym of corynebacterium variabile.strains of a gram-positive bacterium were isolated from the irish smear-ripened cheese gubbeen, and assigned to a new species, corynebacterium mooreparkense, in 2001. during a further study on the same cheese, no additional isolates from this species could be found. instead, multiple isolates of its nearest phylogenetic neighbour, corynebacterium variabile, were found. a first screening with rep-pcr and sds-page pointed to a similarity between c. mooreparkense and c. variabile. following this pe ...200515879244
surface microflora of four smear-ripened cheeses.the microbial composition of smear-ripened cheeses is not very clear. a total of 194 bacterial isolates and 187 yeast isolates from the surfaces of four irish farmhouse smear-ripened cheeses were identified at the midpoint of ripening using pulsed-field gel electrophoresis (pfge), repetitive sequence-based pcr, and 16s rrna gene sequencing for identifying and typing the bacteria and fourier transform infrared spectroscopy and mitochondrial dna restriction fragment length polymorphism (mtdna rflp ...200516269673
rapid analysis of two food-borne microbial communities at the species level by fourier-transform infrared microspectroscopy.the species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. during recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. i ...200616623742
[effect of synthetic surfactants on some biological properties of non-pathogenic species of the genus corynebacterium].the influence of cationic, anionic and non-ionic synthetic surfactants on some biological properties of different representatives of the following species corynebacterium glutamicum, corynebacterium ammoniagenes, corynebacterium vitaeruminis, corynebacterium variabile and strain corynebacterium sp. (brevibacterium stationis) ukm as-719 has been investigated. it has been shown that the action of surfactants is accompanied by the change of cell morphology and loss of ability for gram-staining, as ...200616869145
sources of the adventitious microflora of a smear-ripened cheese.to determine the relationships between the major organisms from the cheese-making personnel and environment and the surface of a smear cheese.200616907817
growth and colour development of some surface ripening bacteria with debaryomyces hansenii on aseptic cheese curd.the growth of five bacteria isolated from red-smear cheeses, brevibacterium aurantiacum, corynebacterium casei, corynebacterium variabile, microbacterium gubbeenense and staphylococcus saprophyticus in mixed cultures with debaryomyces hansenii on aseptic model cheese curd at 10 and 14 degrees c was investigated. at both temperatures, c. casei and micro. gubbeenense had a longer lag phase than c. variabile, brevi. aurantiacum and staph. saprophyticus. in all cultures, lactose was utilised first a ...200616978429
stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese.a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ...200717430918
stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
growth characteristics of brevibacterium, corynebacterium, microbacterium, and staphylococcus spp. isolated from surface-ripened cheese.the growth characteristics of five bacteria, brevibacterium aurantiacum 1-16-58, corynebacterium casei dpc 5298(t), corynebacterium variabile dpc 5310, microbacterium gubbeenense dpc 5286(t), and staphylococcus saprophyticus 4e61, all of which were isolated from the surface of smear cheese, were studied in complex and chemically defined media. all of the coryneforms, except m. gubbeenense, grew in 12% salt, while b. aurantiacum and s. saprophyticus grew in 15% salt. all five bacteria assimilated ...200717921266
diversity and evolution of the microbial populations during manufacture and ripening of casín, a traditional spanish, starter-free cheese made from cow's milk.classical culturing and denaturing gradient gel electrophoresis (dgge) techniques have been used for studying the microbial diversity and dynamics of the traditional spanish casín cheese during manufacturing and ripening. as with other starter-free cheeses made from raw milk, the microbial diversity of casín was shown to be high by both culturing and dgge analyses. the culture technique showed that lactic acid bacteria (lab) species constituted the majority of the microbial populations. of the 1 ...200919822375
corynebacterium nuruki sp. nov., isolated from alcohol fermentation starter.a novel gram-positive, strictly aerobic and non-motile bacterial strain, s6-4(t), was isolated from a korean alcohol fermentation starter. optimal growth occurred at 37 °c, ph 8 and 1 % (w/v) of nacl. positive for oxidase and catalase. the isolate assimilates a various sugars and acids were produced from several carbohydrates. the major cell-wall sugars were galactose and arabinose. the major fatty acids of strain s6-4(t) were c(16:0), c(17:1)ω9c, c(18:1) ω9c and tbsa 10-me-c(18:0) (tuberculoste ...201021075904
Complete genome sequence of Corynebacterium variabile DSM 44702 isolated from the surface of smear-ripened cheeses and insights into cheese ripening and flavor generation.Corynebacterium variabile is part of the complex microflora on the surface of smear-ripened cheeses and contributes to the development of flavor and textural properties during cheese ripening. Still little is known about the metabolic processes and microbial interactions during the production of smear-ripened cheeses. Therefore, the gene repertoire contributing to the lifestyle of the cheese isolate C. variabile DSM 44702 was deduced from the complete genome sequence to get a better understandin ...201122053731
bacteria present in comadia redtenbacheri larvae (lepidoptera: cossidae).the external and internal culturable bacterial community present in the larvae of comadia redtenbacheri hammerschmidt, an edible insect, was studied. characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16s rrna gene revealed the existence of paenibacillus sp., bacillus safensis, pseudomonas sp., bacillus pseudomycoides, corynebacterium variabile, enterococcus sp., gordonia sp., acinetobacter calcoaceticus, arthrobacter sp., micrococcus sp. ...201526336239
identification of microbiota present on the surface of taleggio cheese using pcr-dgge and rapd-pcr.microbial dna from 9 batches of taleggio pdo cheese sampled at various times during ripening, brines, swabs of wooden shelves used for cheese dry-salting, and 13 commercial cheeses were analyzed by denaturing gradient gel electrophoresis (pcr-dgge) and/or random amplification of polymorphic dna (rapd-pcr). sequencing allowed the detection of 12 genera, 27 species, and 2 unclassified bacteria. molecular analysis allowed for the detection of microorganisms not previously associated with taleggio s ...201223094819
degradation of n-alkanes and pahs from the heavy crude oil using salt-tolerant bacterial consortia and analysis of their catabolic genes.in the present study, salt-tolerant strains, dietzia sp. hrj2, corynebacterium variabile hrj4, dietzia cinnamea hrj5 and bacillus tequilensis hrj6 were isolated from the dagang oil field, china. these strains degraded n-alkanes and polycyclic aromatic hydrocarbons (pahs) aerobically from heavy crude oil (hco) in an experiment at 37 °c and 140 rpm. the gc/ms investigation for degradation of different chain lengths of n-alkanes (c8-c40) by individual strains showed the highest degradation of c8-c1 ...201728315056
biodegradation of trichloroethylene and toluene by indigenous microbial populations in vadose sediments.the unsaturated subsurface (vadose zone) receives significant amounts of hazardous chemicals, yet little is known about its microbial communities and their capacity to biodegrade pollutants. trichloroethylene (tce) biodegradation occurs readily in surface soils; however, the process usually requires enzyme induction by aromatic compounds, methane, or other cosubstrates. the aerobic biodegradation of toluene and tce by indigenous microbial populations was measured in samples collected from the va ...199524185349
isolation and characterization of crude oil degrading bacteria from the persian gulf (khorramshahr provenance).fifteen crude oil degrading bacteria were isolated from oil contaminated sites in the persian gulf at khorramshahr provenance. these bacteria were screened with two important factors such as growth rate on crude oil and hydrocarbon biodegradation, and then three strains were selected from 15 isolated strains for further study. one strain (pg-z) that show the best crude oil biodegradation was selected between all isolates. nucleotides sequencing of the gene encoding for 16s rrna show that strain ...201424703768
genome sequence of the squalene-degrading bacterium corynebacterium terpenotabidum type strain y-11(t) (= dsm 44721(t)).corynebacterium terpenotabidum takeuchi et. al 1999 is a member of the genus corynebacterium, which contains gram-positive and non-spore forming bacteria with a high g+c content. c. terpenotabidum was isolated from soil based on its ability to degrade squalene and belongs to the aerobic and non-hemolytic corynebacteria. it displays tolerance to salts (up to 8%) and is related to corynebacterium variabile involved in cheese ripening. as this is a type strain of corynebacterium, this project descr ...201425197436
bioconversion of gibberellin fermentation residue into feed supplement and organic fertilizer employing housefly (musca domestica l.) assisted by corynebacterium variabile.the accumulation of a considerable quantity of gibberellin fermentation residue (gfr) during gibberellic acid a3 (ga3) production not only results in the waste of many resources, but also poses a potential hazard to the environment, indicating that the safe treatment of gfr has become an urgent issue for ga3 industry. the key to recycle gfr is converting it into an available resource and removing the ga3 residue. to this end, we established a co-bioconversion process in this study using house fl ...201525992605
draft genome sequence of corynebacterium variabile mu292, isolated from munster, a french smear-ripened cheese.here, we report the draft genome sequence of corynebacterium variabile mu292, which was originally isolated from the surface of munster, a french smear-ripened cheese. this genome investigation will improve our knowledge on the molecular determinants potentially involved in the adaptation of this strain during the munster-type cheese manufacturing process.201627445372
a novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant corynebacterium variabile hrj4 and biochar.the present work aimed to develop a novel strategy to bioremediate the petroleum hydrocarbon contaminants in the environment. salt tolerant bacterium was isolated from dagang oilfield, china and identified as corynebacterium variabile hrj4 based on 16s rrna gene sequence analysis. the bacterium had a high salt tolerant capability and biochar was developed as carrier for the bacterium. the bacteria with biochar were most effective in degradation of n-alkanes (c16, c18, c19, c26, c28) and polycycl ...201627593267
inferring microbial interactions in the gut of the hong kong whipping frog (polypedates megacephalus) and a validation using probiotics.the concerted activity of intestinal microbes is crucial to the health and development of their host organisms. investigation of microbial interactions in the gut should deepen our understanding of how these micro-ecosystems function. due to advances in next generation sequencing (ngs) technologies, various bioinformatic strategies have been proposed to investigate these microbial interactions. however, due to the complexity of the intestinal microbial community and difficulties in monitoring th ...201728424669
revisiting corynebacterium glyciniphilum (ex kubota et al., 1972) sp. nov., nom. rev., isolated from putrefied banana.a strain of a species of the genus corynebacterium, designated aj 3170(t), was isolated during the 1980s from putrefied bananas. since then, there have been no further updates on the description of the strain or its phylogenetic classification. however, phylogenetic analysis of this strain using 16s rrna and in silico dna-dna hybridization has confirmed that it is a member of the genus corynebacterium and that strain aj 3170(t) clusters with corynebacterium variabile dsm 44702(t), corynebacteriu ...201525323597
biodiversity of the surface microbial consortia from limburger, reblochon, livarot, tilsit, and gubbeen cheeses.comprehensive collaborative studies from our laboratories reveal the extensive biodiversity of the microflora of the surfaces of smear-ripened cheeses. two thousand five hundred ninety-seven strains of bacteria and 2,446 strains of yeasts from the surface of the smear-ripened cheeses limburger, reblochon, livarot, tilsit, and gubbeen, isolated at three or four times during ripening, were identified; 55 species of bacteria and 30 species of yeast were found. the microfloras of the five cheeses sh ...201426082119
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