[peptidoglycan type and cell wall polysaccharide composition of cellulomonas cartalyticum and some coryneform organisms (author's transl)].cellulomonas cartalyticum was found to contain a peptidoglycan type different from that of the other species of cellulomonas. the diamino acid is lysin instead of ornithine and the interpeptide bridge consists of d-asp-d-ser. the same peptidoglycan type occurs in corynebacterium manihot, brevibacterium liticum and arthrobacter luteus. these non cellulolytic organisms are most likely not closely related with cellulomonas cartalyticum, as indicated by the very different g +c content of their dna, ...197898123
[brevibacterium fermentans: 3 strains isolated in dakar]. 19714998495
preparation and characterization of yeast nuclear extracts for efficient rna polymerase b (ii)-dependent transcription in vitro.we present a reproducible method for the preparation of nuclear extracts from the yeast saccharomyces cerevisiae that support efficient rna polymerase b (ii)-dependent transcription. extracts from both a crude nuclear fraction and percoll-purified nuclei are highly active for site-specific initiation and transcription of a g-free cassette under the adenovirus major late promoter. at optimal extract concentrations transcription is at least 5 times more efficient with the yeast extracts than with ...19902263463
cellular fatty acid composition of oerskovia species, cdc coryneform groups a-3, a-4, a-5, corynebacterium aquaticum, listeria denitrificans and brevibacterium acetylicum.twenty four strains representing eight species of gram positive yellow-pigmented rods (oerskovia turbata, oerskovia xanthineolytica, cdc coryneform groups a-3, a-4, a-5, listeria denitrificans, corynebacterium aquaticum and brevibacterium acetylicum) were divided into two major groups based on the relative amounts of 12 methyltetradecanoate (15:0a) obtained by capillary gas liquid chromatography. o. turbata, o. xanthineolytica, cdc groups a-3 and a-4, l. denitrificans and c. aquaticum were place ...19902124793
endophthalmitis due to oerskovia xanthineolytica.unilateral endophthalmitis developed in a 47-year-old man following an intraocular eye injury with a metallic foreign body. eventually, oerskovia xanthineolytica was isolated from the vitreous humour. to our knowledge this is the first documented case of endophthalmitis caused by this organism in humans. the clinical course and management of this opportunistic infection are discussed.19873496945
[postoperative meningitis and prolonged fever. repeated isolation of brevibacterium fermentans. pathogenic hypotheses]. 19695192436
oerskovia xanthineolytica and methicillin-resistant staphylococcus aureus in a patient with cirrhosis and variceal hemorrhage. 19921486895
meningitis caused by oerskovia summary, we describe a case of central nervous system infection with o. xanthineolytica in which the infecting microbe probably was engrafted on a ventricular shunt. the bacteria caused a smoldering meningitis that did not respond to penicillin and rifampin despite in vitro sensitivity, presumably because of inadequate cerebrospinal fluid penetration of the penicillin and the recognized difficulty of eradicating bacteria from contaminated shunts. removal of the shunt and continued treatment w ...19883354593
comparative immunological study of catalases in the genus micrococcus.double immunodiffusion tests were performed with crude extracts from various micrococcus species and antisera against catalase of micrococcus luteus ccm 169. cell-free extracts of m. lylae atcc 27566 exhibited good cross-reaction. cell-free extracts or catalase enriched preparations of m. varians reacted very weakly and no reaction has been found with preparation of m. kristinae, m. nishinomiyaensis, m. roseus and m. sedentarius. the quantitative microcomplement fixation assay also revealed a cl ...197771880
primary sequence of the glucanase gene from oerskovia xanthineolytica. expression and purification of the enzyme from escherichia coli.a 2.7-kilobase fragment of dna from oerskovia xanthineolytica containing the gene for a beta-1,3-glucanase has been isolated and its complete nucleotide sequence determined. the sequence was found to contain two large open reading frames. purification of the mature native enzyme and subsequent amino-terminal sequencing defined the glucanase gene in one reading frame which potentially encodes a protein of 548 amino acids. we have expressed this glucanase gene in escherichia coli under control of ...19911985933
changes in fatty acid branching and unsaturation of streptomyces griseus and brevibacterium fermentans as a response to growth temperature.streptomyces griseus showed three different modes of changing fatty acids in response to temperature change. in brevibacterium fermentans, two such responses were found. the responses involved changes in fatty acid branching, unsaturation, or chain length, depending on growth temperature range. changes in unsaturation of branched-chain acids were characteristic at low growth temperatures.19921637171
specific cleavage and physical mapping of simian-virus-40 dna by the restriction endonuclease of arthrobacter luteus.simian virus 40 (sv40) dna (strain 776) is cleaved by the restriction endonuclease from arthrobacter luteus into 32 specific fragments including 20 large pieces designated alu-a through t as well as 12 minor products named alu m1 through m8. these were mapped on the sv40 genome by double digestion experiments. alu fragments were treated with hind enzymes and vice versa. similar reciprocal digestions were also carried out with hae iii enzyme. in this way a detailed cleavage map of the sv40 genome ...1976173549
arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 rf dna. 19751081295
molecular structure of rarobacter faecitabidus protease i. a yeast-lytic serine protease having mannose-binding activity.rarobacter faecitabidus protease i (rpi) is a serine protease exhibiting lytic activity toward living yeast cells. rpi is similar to elastase in its substrate specificity and has a lectin-like affinity for mannose. the gene encoding rpi was cloned to elucidate its structure and function. and its nucleotide sequence revealed that it contains an open reading frame encoding a 525-amino acid protein. homology comparison indicated that pre-pro-rpi consists of three domains: (1) an nh2-terminal prepro ...19921339445
lyticase: endoglucanase and protease activities that act together in yeast cell lysis.yeast lytic activity was purified from the culture supernatant of oerskovia xanthineolytica grown on minimal medium with insoluble yeast glucan as the carbon source. the lytic activity was found to consist of two synergistic enzyme activities which copurified on carboxymethyl cellulose and sephadex g-150, but were resolved on bio-gel p-150. the first component was a beta-1,3-glucanase with a molecular weight of 55,000. the k(m) for yeast glucan was 0.4 mg/ml; that for laminarin was 5.9 mg/ml. hy ...19806991473
the nucleotide sequence of a dna fragment, 71 base pairs in length, near the origin of dna replication of bacteriophage 0x174.part of the nucleotide sequence of a restriction fragment covering the origin of phix174 dna replication 1 has been determined. the fragment a7c was obtained by digestion of phix174 rf dna by the restriction enzyme from arthrobacter luteus, alu 1. it was further cleaved into two fragments, one large and one small, by the action of the restriction enzyme from haemophilus aegyptius, hae 111. the nucleotide sequence of the small fragment has been determined by analysis of the transcription products ...1976995652
dextranase activity in oerskovia xanthineolytica. 1976939734
interaction of bacteriophage o2 with strains of the genus oerskovia.bacteriophage o2 multiplies normally on oerskovia turbata imet 47 153. it has a burst size of about 100 p.f.u. per infected cell and a latent period of 100 min at 30 degrees c. on oerskovia xanthineolytica imet 47 383 clear spots were formed after addition of high phage concentrations onto agar top layers. by phase contrast observation, and measurement of the optical density of infected cultures, it was found that the clearing effect on strain imet 47 383 was due to lysis-from-without. phage o2 ...19817293244
identification of some clinical strains of cdc coryneform group a-3 and a-4 bacteria as cellulomonas species and proposal of cellulomonas hominis sp. nov. for some group a-3 strains.cdc coryneform group a-3 and a-4 bacteria were defined by hollis and weaver in 1981, but their taxonomic position is still unclear. by using biochemical and chemotaxonomical methods, four clinical strains belonging to cdc coryneform groups a-3 (n = 2) and a-4 (n = 2) were studied and could be assigned to the genus cellulomonas, resulting in the first description of cellulomonas strains isolated from clinical specimens. cdc coryneform group a-3 and a-4 strains were compared with the type strains ...19957559954
physical mapping of bk virus dna with saci, mboii, and alui restriction endonucleases.a new restriction endonuclease, saci from streptomyces achromogenes cleaves bk virus (strain mm) dna into 3 fragments, whereas mboii from moraxella bovis and alui from arthrobacter luteus give 22 and 30 fragments, respectively. all these specific dna fragments were ordered and mapped on the viral genome by two methods first, by the reciprocal digestion method using uniformly 32p-labeled dna; and second, by the partial digestion technique using the single-end 32p-labeled dna. this study, together ...1978215783
[dna-methylase from arthrobacter luteus screens dna from the action of site-specific endonuclease alu i].dna-methylase was isolated from a cell extract of a. luteus and partially purified by chromatography on phosphocellulose. the purified enzyme methylates dna of phage lambda and plasmids pbr322, thus making them resistant to a subsequent action of endonuclease alu i. it has been shown that cytosine is the object of methylation within dna. this modification does not screen dna from the action of site-specific endonucleases sal i, bam hi, ecor i, ecor ii, xho i and xho ii. it has been experimentall ...19816268201
the m.alui dna-(cytosine c5)-methyltransferase has an unusually large, partially dispensable, variable region.the dna methyltransferase of the alui restriction-modification system, from arthrobacter luteus, converts cytosine to 5-methylcytosine in the sequence agct. the gene for this methyltransferase, aluim, was cloned into escherichia coli and sequenced. a 525-codon open reading frame was found, consistent with deletion evidence, and the deduced amino acid sequence revealed all ten conserved regions common to 5-methylcytosine methyltransferases. the aluim sequence predicts a protein of m(r) 59.0k, in ...19938451189
[arthrobacter proteinase and its effect on fibrin thrombi].arthrobacteria are producers of proteolytic enzymes. optimal cultivation conditions were determined for arthrobacter luteus and the mineral composition of the culture medium providing active synthesis of proteinase was optimized. the proteinase was isolated from the culture liquid of arthrobacter luteus using preparative ion-exchange chromatography on a carboxyl cation exchange resin kmt. the enzyme was homogeneous according to gel chromatography on sephadex g-200 and had a molecular weight of 2 ...19938475025
peritonitis caused by oerskovia xanthineolytica in a patient on chronic ambulatory peritoneal dialysis (capd) 19968604720
lysis of viable yeast cells by enzymes of arthrobacter luteus. 19715166255
arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of sv40 dna.the nucleotide sequence at the cleavage site of the restriction endonuclease isolated from arthrobacter luteus (alu) has been determined. the endonuclease cleaves at the center of a palindromic tetranucleotide sequence to give even-ended duplex dna fragments phosphorylated at the 5'-end. the endonuclease cleaves sv40 form i dna into 32 fragments. the order and sizes of these fragments have been determined to provide an alu cleavage map of the sv40 genome.1976182213
mapping and length measurements of restriction enzyme fragments by electron microscopy.1. we have mapped by electron microscopy the dna-fragments formed by the action of the restriction endonuclease from arthrobacter luteus of phi x 174 replicative form dna. these fragments were separated by polyacrylamide gel electrophoresis and hybridized to phix 174 single stranded dna. the partial duplex molecules were inspected in the electron microscope. in this way the relative order of eleven fragments ranging in size from approximately 100 to 1000 nucleotide pairs has been established and ...1977321023
analysis of morphogenesis of the nocardioform organism oerskovia xanthineolytica.depending on the nutritional and physicochemical conditions of growth the shape of oerskovia xanthineolytica varied within a broad range. in different exponentially growing cultures five morphological types could be distinguished. in liquid cultures with increasing growth rate oerskovia grew either as rods, filaments or branched filaments, whereas for the agar-microcultures pseudomycelia, but under reduced aeration mycelia were typical. the morphogenetic parameters of each type were determined, ...19817269655
nucleotide sequence of a beta-1,3-glucanase isoenzyme iia gene of oerskovia xanthineolytica ll g109 (cellulomonas cellulans) and initial characterization of the recombinant enzyme expressed in bacillus subtilis.the nucleotide sequence of the betagliia gene, encoding the extracellular beta-1,3-glucanase iia (betagliia) of the yeast-lytic actinomycete oerskovia xanthineolytica ll g109, was determined. sequence comparison shows that the betagliia enzyme has over 80% identity to the betaglii isoenzyme, an endo-beta-1,3-glucanase having low yeast-lytic activity secreted by the same bacterium. the betagliia enzyme lacks a glucan- or mannan-binding domain, such as those observed in beta-1,3-glucanases and pro ...19968755914
oerskovia xanthineolytica infection of a prosthetic joint: case report and review.oerskovia spp. are gram-positive, nocardia-like bacilli which inhabit the soil and rarely cause human infections. previously reported cases of oerskovia infection have been characterized by a nonaggressive course and an association with foreign bodies. we report the first case of a patient with a prosthetic joint infection due to oerskovia xanthineolytica. our patient presented with a prolonged, indolent course and was thought to have aseptic loosening of his prosthesis until the time of surgery ...19968784601
phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of sanguibacter gen.nov.16s rrna gene sequencing studies were performed on some gram-positive coryneform bacteria of unknown taxonomic position isolated from blood and milk of healthy cows. comparative sequence analysis demonstrated that the milk isolates corresponded to oerskovia xanthineolytica whereas those from blood consisted of two distinct, albeit highly related species, within the family cellulomonadaceae. based on the phylogenetic and phenotypic distinctiveness of the blood isolates, it is proposed that they b ...19957765903
oerskovia xanthineolytica implicated in peritonitis associated with peritoneal dialysis: case report and review of oerskovia infections in humans.oerskovia species are nocardialike bacteria that have been implicated as pathogens only rarely. these organisms are branched, gram-positive bacilli that are oxidase negative, catalase positive, and non-acid fast. unlike nocardia species, these organisms are motile, do not produce aerial mycelia, and possess a cell wall with large amounts of galactose. colonies are bright yellow and produce branched vegetative hyphae on nutrient agar. a 70-year-old patient undergoing chronic ambulatory peritoneal ...19902229375
oerskovia xanthineolytica keratitis. 19968728613
evolutionary relationship between halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-trna synthetases as phylogenetic probes.the cross-species reactivities between trnas and aminoacyl-trna synthetases have been employed as a basis to estimate the relatedness of various prokaryotes to the eukaryotes. the trna of halobacterium cutirubrum, unlike that of other prokaryotes tested, including agrobacterium tumefaciens, arthrobacter luteus, bacillus subtilis, bacillus stearothermophilus, escherichia coli, micrococcus luteus, myxococcus xanthus, rhodopseudomonas spheroides, and thermus aquaticus, was found to share with yeast ...19806989454
oerskovia xanthineolytica peritonitis: case report and review. 199910462352
molecular cloning of a lytic beta-1,3-glucanase gene from oerskovia xanthineolytica llg109. a beta-1,3-glucanase able to selectively permeabilize the yeast cell wall.molecular cloning of the beta giii gene encoding for an endo-beta-1,3-glucanase (beta gl ii) from oerskovia xanthineolytica llg109, a yeast-lytic gram-positive bacterium, has been conducted in order to elucidate its primary sequence and subsequently express it into b. subtilis. this endo-beta-1,3-glucanase exhibits low yeast-lytic activity toward viable s. cerevisiae cells, and it has shown ability to selectively permeabilize the yeast cell wall and release intracellular proteins produced by yea ...19968659924
streamlined beta-galactosidase assay for analysis of recombinant yeast response to, we describe a rapid, convenient, and quantitative beta-galactosidase assay in liquid culture of recombinant yeast that expresses the estrogen receptor. this assay allows large-scale screening of chemicals (more than 600 samples/day) for the evaluation of their direct estrogenic potency and accurate determination of their ec50 with minimal manipulations. the assay, which is based on digestion of the yeast cell wall by lyticase (zymolase), a beta-glucanase isolated from arthrobacter luteus, ...200111355334
reclassification of cellulomonas cellulans (stackebrandt and keddie 1986) as cellulosimicrobium cellulans gen. nov., comb. nov.phylogenetic analysis of 16s rdna provides evidence that cellulomonas cellulans branches outside the phylogenetic confines of the genus cellulomonas. the distinct phylogenetic and chemotaxonomic status of cellulomonas cellulans as a phylogenetic neighbour of the genus promicromonospora, justifies the description of a new genus for which the name cellulosimicrobium gen. nov. with the type species cellulosimicrobium cellulans comb. nov. is proposed.200111411667
cleavage map of bacteriophage phix174 rf dna by restriction enzymes.phix rf dna was cleaved by restriction enzymes from haemophilus influenzae rf (hinf i) and haemophilus haemolyticus (hha. i). twenty one fragments of approximately 25 to 730 base pairs were produced by hinf i and seventeen fragments of approximately 40 to 1560 base pairs by hha i. the order of these fragments has been established by digestion on haemophilus awgyptius (hae iii) and arthrobacter luteus (alu i) endonuclease fragments of phix rf with hinf i and hha1. by this method of reciprocal dig ...19761085927
overproduction, purification, and characterization of beta-1,3-glucanase type ii in escherichia escherichia coli recombinant system produced soluble and full-length beta-1,3-glucanase type ii (bglii) cloned from the yeast-lytic actinomycete oerskovia xanthineolytica. the expression system was designed to produce recombinant bglii with a six-histidine peptide fused to the carboxy end of the protein. the expression level was optimized to produce 30% of total protein of e. coli as the recombinant protein, releasing 75% to the extracellular space. the 43-kda recombinant protein was purified ...200111676595
isolation and identification of cellulolytic bacteria involved in the degradation of natural cellulosic search for bacterial cultures that are able to rapidly degrade cellulosic plant fibres in vitro, 77 cellulolytic strains were isolated from belgian and czech soils after enrichment on flax or sisal fibres as sole sources of carbon. the strains were characterized using fatty acid analysis, and 74 strains were grouped into three major clusters by numerical analysis. the first major cluster contained cellulomonas strains. within this cluster three subclusters could be delineated by principal com ...200010930083
application of capillary electrophoresis to monitor populations of cellulomonas cartae kym-7 and agrobacterium tumefaciens kym-8 in mixed culture.a bacterial cell mixture ot cellulomonas cartae kym-7 and agrobacterium tumefaciens kym-8 was analyzed by capillary zone electrophoresis (cze) and capillary gel electrophoresis (cge). both pherograms showed two discrete peaks. the cells in the peaks were collected, gram stained, and examined with a microscope. the cells of the two strains were well separated by oge, and each oge peak consisted almost entirely of cells of one strain (greater than 98% purity), whereas each cze peak contained cells ...200111669519
extracellular acidic polysaccharide production by a two-membered bacterial coculture.a two-membered coculture of strains kym-7 and kym-8, identified as cellulomonas cellulans and agrobacterium tumefaciens, respectively, produced a large amount of an extracellular polysaccharide, designated apk-78, from starch. each strain in pure culture produced only very little amount of polysaccharide from starch; the coexistence of the two strains from the early stage of cultivation was indispensable for a large amount of polysaccharide to be produced. the polysaccharide apk-78 was acidic an ...200312619667
[the existence of contamination in enzymes used for the isolation of aspergillus and candida species dna].molecular biological methods which are widely used in different fields, have been replaced with conventional diagnostic tests for the early diagnosis of invasive fungal infections, recently. polymerase chain reaction (pcr) is one of these methods, with high specificity and sensitivity, which is accepted throughout the world. however, the enzymes that are used for the isolation of target dna, may be contaminated with the gene sequences of some other fungal species in the preparation steps and may ...200212652873
isolation of oerskovia xanthineolytica from an aborted equine foetus. 19826889853
effective extracellular trehalose production by cellulosimicrobium cellulans.a bacterium isolated from a petal of casa blanca lily (st26 strain) produced a marked amount of extracellular trehalose (alpha- d-glucopyranosyl-[1,1]-alpha- d-glucopyranose) in culture medium containing glucose. 16s rdna-based phylogeny showed that st26 belongs to, or is related to, cellulosimicrobium cellulans, a close relative of cellulomonas spp. various cellulomonas strains obtained from culture collections also showed extracellular trehalose productivity, suggesting that trehalose producti ...200414685787
oerskovia xanthineolytica endocarditis in a renal transplant patient: case report and review of the literature.oerskovia species were, until recently, only rarely associated with human disease. this gram-positive bacillus can be easily misidentified as a diphtheroid, a common contaminant in blood cultures. there have been 17 reports of invasive oerskovia infection in immunocompromised hosts. we report a case of oerskovia xanthineolytica endocarditis in a renal transplant patient and review the microbiologic and clinical characteristics of this potential pathogen.200314987205
oerskovia xanthineolytica bacteremia in an immunocompromised patient with pneumonia.oerskovia species, which are nocardia-like bacteria that have rarely been found to cause human disease, are usually found in association with a foreign body with removal of the infected focus being necessary for cure. we present a case of oerskovia xanthineolytica bacteremia in a patient with metastatic breast cancer, community-acquired pneumonia, and a tunneled subcutaneous central venous catheter. although the actual source of the bacteremia in this case is not proven, this patient's presentat ...19947924223
restriction-enzyme-cleavage maps of bacteriophage m13. existence of an intergenic region on the m13 genome.replicative form dna of bacteriophage m13 was cleaved into specific fragments by an endonuclease isolated from hemophilus aegyptius (endor.haeii) and an endonuclease from arthrobacter luteus (endor.alui). the fragments were ordered as to construct a circular map of the phage m13 genome by: (a) using each fragment as a primer for the synthesis in vitro of its respective neighbour and (b) digesting the isolated fragments with the hemophilus aegyptius enzyme endor.haeii or the hemophilus aphirophil ...1976786638
production and ecological significance of yeast cell wall-degrading enzymes from oerskovia.motile actinomycetes capable of degrading walls of viable yeast cells were isolated from soil and identified as oerskovia xanthineolytica. a lytic assay based on susceptibility of enzyme-treated cells to osmotic shock was developed, and 10 of 15 strains of o. xanthineolytica, oerskovia turbata, and nonmotile oerskovia- like organisms from other collections were found to possess yeast lytic activities. all lytic strains produced laminaranase and alpha-mannanase, but the amounts, determined by red ...197816345321
oerskovia xanthineolytica bacteremia in an immunocompromised patient without a foreign body. 200312709844
oerskovia xanthineolytica bacteremia in an immunocompromised host: case report and review.oerskovia species, once thought to be nocardiform-like bacteria, have been only rarely associated with human infection. in this report we describe a case of central venous catheter-associated infection that was successfully treated with antibiotics. with the increased use of indwelling devices, these organisms may be more commonly recognized causes of infection. appropriate antibiotic therapy appears to successfully treat oerskovia infection and may decrease the need for removal of some indwelli ...19968852977
cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite mastotermes darwiniensis.a novel cellulolytic and xylanolytic bacterium, strain mx5t, was isolated from the hindgut contents of the australian termite mastotermes darwiniensis (froggatt). the isolate was a facultative anaerobe and had a gram-positive cell-wall profile. the rod-shaped bacterium formed irregular coryneform and coccoid cells during growth. phylogenetic analysis of the 16s rdna provided evidence that the organism was closely related to the as-yet undescribed cellulolytic strain sr272 and the non-validly des ...200212148626
oerskovia xanthineolytica: a new pathogen in bone marrow transplantation.a 53-year-old woman with non-hodgkin lymphoma underwent an autologous bone marrow transplant (bmt). incomplete reconstitution necessitated the use of a long-term central venous catheter. one year after bmt she presented with fever. echocardiography revealed vegetations on the tricuspid valve. gram-positive rods grown from blood cultures and catheter tip were identified as oerskovia xanthineolytica. we report the first case of native valve endocarditis caused by this organism.19989733276
reclassification of cellulosimicrobium variabile bakalidou et al. 2002 as isoptericola variabilis gen. nov., comb. already depicted in the original publication, the type strain of the species cellulosimicrobium variabile bakalidou et al. 2002, dsm 10177(t), does not cluster unambiguously with the type species, cellulosimicrobium cellulans dsm 43879(t), in phylogenetic analysis. strain dsm 10177(t) is moderately related to the recently described species xylanimonas cellulosilytica, promicromonospora pachnodae and xylanibacterium ulmi, forming a lineage that branches between c. cellulans and members of the ...200415143008
potential misidentification of a new exiguobacterium sp. as oerskovia xanthineolytica isolated from blood culture. 200616872001
[polyphasic evidence for the transfer of promicromonospora yunnanensis to cellulosimicrobium cellulans].polyphasic taxonomic investigations of promicromonospora yunnanensis as4.1333 deposited in the china general microbiological culture collection center (cgmcc) indicated that strain as4.1333 was closely related to cellulosimicrobium cellulans dsm43879(t), the two organisms shared a 16s rrna gene similarity of 99.6% which correspond to 5 nt differences at 1423 positions. corresponding dna-dna reassociation value was 89.3%, significantly higher than 70% cut-off point recommended for the delineation ...200617037045
transformation of adenine to 8-hydroxyadenine by strains of oerskovia xanthineolytica.the 8-hydroxy derivative of adenine (6-amino-1,7-dihydro-8h-purin-8-one) is produced from adenine by two oerskovia xanthineolytica strains. this transformation by a microorganism has not been reported previously. no novel products of dissimilation of xanthine (3,7-dihydro-1h-purine-2,6-dione) or hypoxanthine (1,7-dihydro-6h-purin-6-one) were found. xanthine was oxidized to uric acid, but intermediates in the breakdown of hypoxanthine could not be demonstrated.198216345943
diffusion characteristics and controlled release of bacterial fertilizers from modified calcium alginate indigenous cellulosimicrobium cellulans gs6 isolate able to solubilize insoluble phosphate complexes in soil is a potential bacterial fertilizer. enclosure of the phosphate-solubilizing bacterium (psb) in biodegradable capsules may protect the psb cells inoculated into soil and, in the meantime, enable the control of cell release that confers long-term fertilizing effects. in this study, calcium alginate (ca) was used as the core matrix to encapsulate cells of c. cellulans gs6. the cell-liber ...200817482812
cellulosimicrobium terreum sp. nov., isolated from soil.a gram-positive, yellow-pigmented, non-motile and rod-shaped or coccoid bacterial strain, ds-61t, was isolated from soil from dokdo, korea, and its taxonomic position was investigated by using a polyphasic approach. the strain grew optimally at ph 6.5-7.5 and 25 degrees c in the presence of 1.0% (w/v) nacl. strain ds-61t had peptidoglycan of the type based on l-lys-l-thr-d-asp and contained galactose as the only whole-cell sugar. mk-9(h4) was the predominant menaquinone and anteiso-c15:0 and iso ...200717978207
kinetics of enzymatic lysis and disruption of yeast cells: i. evaluation of two lytic systems with different properties.many microorganisms produce enzymes which lyse the walls of yeasts, fungi, and bacteria. the proportions of different enzyme activities present in the lytic system, their action patterns, synergism, and dependence on inhibitors, constitute the activity profile of the lytic system. taken together, the activity profile and process conditions for lysis determine the reaction rate and the distribution of products from lysis of any given type of cells. kinetics of glucan hydrolysis, proteolysis, and ...198718581424
continuous-culture studies of synthesis and regulation of extracellular beta(1-3) glucanase and protease enzymes from oerskovia xanthineolytica.this article describes the synthesis and regulation of beta(1-3)glucanase and protease enzymes from the cell lytic system of oerskovia xanthineolytica ll-g109 in continuous culture using different concentrations of carbon source (glucose) and inducer (glucan). these two enzyme activities are the main components of a lytic system capable of lysing and disrupting whole yeast cells; it is subject to catabolite repression by glucose and is induced by yeast glucan. peaks of beta(1-3)glucanase and pro ...198718581450
pyogenic flexor tenosynovitis associated with cellulosimicrobium cellulans.cellulosimicrobium cellulans, formerly known as oerskovia xanthineolytica, is a rare human pathogen, often in association with a foreign body. a case of pyogenic flexor tenosynovitis associated with c. cellulans in an immunocompetent boy is described, underlining the importance of prompt surgical and microbiologic evaluation.200818832122
isolation of bacteria which produce yeast cell wall-lytic enzymes and their isolate bacteria capable of producing enzymes that lyse cell wall, we screened soil samples from rokko mountain where is situated in west japan. the isolated strains kh1, kh2 and kh3 that secrete yeast cell wall lytic enzymes are described herein. the activity of the enzymes, after isolation from culture supernatants, were examined. the lytic enzyme produced by strain kh3 was the most active in both cell wall degradation and beta-1,3 glucanase activity in comparison with commercially availabl ...200818839628
bacterial communities of tyre monofill sites: growth on tyre shreds and investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria.200919239530
cloning and sequence analysis of genes encoding xylanases and acetyl xylan esterase from streptomyces thermoviolaceus opc-520.three genes encoding two types of xylanases (stx-i and stx-ii) and an acetyl xylan esterase (stx-iii) from streptomyces thermoviolaceus opc-520 were cloned, and their dna sequences were determined. the nucleotide sequences showed that genes stx-ii and stx-iii were clustered on the genome. the stx-i, stx-ii, and stx-iii genes encoded deduced proteins of 51, 35.2, and 34.3 kda, respectively. stx-i and stx-ii bound to both insoluble xylan and crystalline cellulose (avicel). alignment of the deduced ...19979023944
study of thermostable chitinases from oerskovia xanthineolytica ncim 2839.the mesophilic organism, oerskovia xanthineolytica ncim 2839, was adapted to grow at moderate thermophilic temperatures. at these elevated temperatures, it was found to produce two thermostable chitinases--c1 and c2. these were purified by ion exchange chromatography using deae cellulose. the chitinases c1 and c2 were found to be stable in a ph range from 3.0 to 9.0 with 7.5 and 8.0 being the optimum ph, respectively. the optimum temperatures of the activities of c1 and c2 were 50 and 55 degrees ...201020091303
a cluster of acute-onset postoperative endophthalmitis over a 1-month period: investigation of an outbreak caused by uncommon report the clinical course, treatment response and prognosis of eight cases which developed acute-onset postoperative endophthalmitis over a 1-month period.201120733020
phenotypic and molecular identification of cellulosimicrobium cellulans isolated from antarctic snow.we report for the first time the isolation of cellulosimicrobium cellulans from antarctic snow. this strain demonstrated physiological traits that were markedly different from that of the mesophilic c. cellulans type strain dsm 43879(t). the dominant cell wall sugars in c. cellulans were glucose, galactose and mannitol whereas rhamnose was the only major sugar in the type strain. cellular fatty acid patterns were dominated by 12-methyltetradecanoic acid (ai-c(15:0)), hexadecanoic acid (c(16:0)) ...200919760124
clearance of cellulosimicrobium cellulans bacteremia in a child without central venous catheter removal.cellulosimicrobium cellulans (formerly known as oerskovia xanthineolytica) rarely causes human infection. infections have been reported in immunocompromised hosts or in patients with foreign bodies, such as catheters, where treatment has generally involved removal of the foreign body. we report on a case in which the organism was isolated in multiple blood cultures from a 13-year-old male. after initial therapy failed, treatment with vancomycin and rifampin resulted in infection clearance withou ...200616825406
efficiency of lyticase (bacterial enzyme) in experimental candidal vaginitis in mice.lyticase (a bacterial enzyme) was tested as a new antimycotic drug. of all objects studied, cellulomonas cellulans ac-870 strain proved to be most productive for this enzyme. a technology for lyticase isolation and purification was proposed. an experimental model of recurrent vaginal candidiasis was created. the model includes combined antibiotic and estradiol therapy. antimycotic effect of lyticase on the model of recurrent vaginal candidiasis in mice was demonstrated.201021165431
revisiting the cellulosimicrobium cellulans yeast-lytic beta-1,3-glucanases toolbox: a review.cellulosimicrobium cellulans (also known with the synonyms cellulomonas cellulans, oerskovia xanthineolytica, and arthrobacter luteus) is an actinomycete that excretes yeast cell wall lytic enzyme complexes containing endo-beta-1,3-glucanases [ec and] as key constituents. three genes encoding endo-beta-1,3-glucanases from two c. cellulans strains have been cloned and characterised over the past years. the betaglii and betagliia genes from strain dsm 10297 (also known as o. xanth ...200616545129
[mitigating the repress of cinnamic acid to cucumber growth by microbial strain].cucumber is one of the most important vegetable species. its continuous planting has become a common practice demand in many areas of china, but an obstacle from continuous planting made sustainable production of this crop to be prohibited. the self-toxic effect was considered as an important negative factor to continuous cropping cucumber. and cinnamic acid was found to be the main substance to cause self-toxic. strain ha8, which isolated from waste water estuary in zhuhai city and has been aut ...200617302157
characterization of clinical isolates previously identified as oerskovia turbata: proposal of cellulosimicrobium funkei sp. nov. and emended description of the genus cellulosimicrobium.taxonomic studies were performed on 13 clinical isolates (ten of which were epidemiologically related) that had been previously identified as oerskovia turbata. comparative phylogenetic analysis, based on 16s rrna gene sequences, indicated that the isolates are closely related to cellulosimicrobium cellulans with sequence similarity values ranging from 99.5 to 99.8 %. chemotaxonomic results (fatty acid profiles and menaquinones) supported the inclusion of these isolates in the genus cellulosimic ...200616585698
efficient conversion from polysialogangliosides to monosialotetrahexosylganglioside using oerskovia xanthineolytica yz-2.a new sialidase-producing strain isolated from soil was identified as oerskovia xanthineolytica yz-2. sialidase was produced when oerskovia xanthineolytica yz-2 was exposed to polysialogangliosides. the sialidase of oerskovia xanthineolytica yz-2 hydrolyzed sialic acid linkages in polysialogangliosides, and released monosialotetrahexosylganglioside (gm1). the sialidase had the capability of product specificity because it did not attack the sialic acid linkage in gm1. therefore, oerskovia xanthin ...201021153421
[meningitis caused by oerskovia xanthineolytica].oerskovia species are nocardia-like bacteria that have been only and rarely associated with human infections. in this report, a meningitis case caused by oerskovia xanthineolytica was presented. a 44 years old male patient who had experienced ventriculo-peritoneal shunt (v-p shunt) due to hydrocephaly, developed meningitis. o. xanthineolytica was isolated from three of the cerebrospinal fluid (csf) samples and v-p shunt cultures. identification of the isolate was performed by phoenix systemtm (n ...200616775964
Critical roles of Asp270 and Trp273 in the a-repeat of the carbohydrate-binding module of endo-1,3-ß-glucanase for laminarin-binding avidity.A carbohydrate-binding module from family 13 (CBM13), appended to the catalytic domain of endo-1,3-ß-glucanase from Cellulosimicrobium cellulans, was overexpressed in E. coli, and its interactions with ß-glucans, laminarin and laminarioligosaccharides, were analyzed using surface plasmon resonance biosensor and isothermal titration calorimetry. The association constants for laminarin and laminarioligosaccharides were determined to be approximately 10(6) M(-1) and 10(4) M(-1), respectively, indic ...201122198269
Molecular characterization of endo-1,3-ß-glucanase from Cellulosimicrobium cellulans: Effects of carbohydrate-binding module on enzymatic function and stability.An endo-1,3-ß-glucanase was purified from Tunicase®, a crude enzyme preparation from Cellulosimicrobium cellulans DK-1, and determined to be a 383-residue protein (Ala1-Leu383), comprising a catalytic domain of the glycoside hydrolase family 16 and a C-terminal carbohydrate-binding module family 13. The Escherichia coli expression system of the catalytic domain (Ala1-Thr256) was constructed, and the protein with N-terminal polyhistidine tag was purified using a Ni-nitrilotriacetic acid column. W ...201121979581
[spondylodiscitis].the authors present a clinical case of a female child, aged 23 months, previously healthy that 24 hours after falling down started to refuse walking and complained about back pain. she never had fever. she felt better with nonsteroidal antiinflammatory drugs, restarting the symptoms, two days after therapy suppression. at admission on hospital she presented inability to flex the lower back and loss of lumbar lordosis, being normal the remaining physical examination. there was a raise of erythroc ...200322226217
traumatic endophthalmitis due to cellulosimicrobium cellulans.purpose. to report a case of traumatic endophthalmitis due to cellulosimicrobium cellulans. design. case report. methods. retrospective chart review. results. to our knowledge, this is the first report of traumatic endophthalmitis due to c. cellulans, which did not respond to intravitreal antibiotics or repeat vitrectomy and ultimately led to the discovery of an occult intraocular foreign body. conclusions. c. cellulans is a rare cause of endophthalmitis. greater awareness of this bacterium in t ...201122606461
lignocellulolytic enzyme production from submerged fermentation of paddy straw.five strains of cellulolytic bacteria and four strains of phanerochaete chrysosporium were evaluated for the lignocellulolytic enzyme production during submerged fermentation (smf) of paddy straw. extra-cellular enzyme assay for cmcase, fpase, cellobiase, xylanase, lignin peroxidase and laccase enzymes was performed after 7 and 15 days of submerged fermentation. cellulomonas cellulans mtcc 23, cytophaga hutchinsonii ncim 2338 and phanerochaete chrysosporium mtcc 787 were found to produce higher ...200723100663
comparative analysis of three guinea pig satellite dna's by restriction nucleases.the structures of guinea pig satellite dnas i, ii, and iii have been analyzed by digestion with seven restriction nucleases. from the cleavage patterns it is obvious that the long-range periodicities in these three satellites differ rather characteristically satellite i is fairly resistant to six nucleases and gives only a number of weak discrete bands which do not show a simple regularity. by the restriction nuclease from arthrobacter luteus, however, it is cleaved extensively and yields very h ...1977403072
a specific endonuclease from arthrobacter luteus. 19761271462
equine abortion and premature birth associated with cellulosimicrobium cellulans infection.during the 2002 and 2003 foaling seasons, cellulosimicrobium (cellumonas) cellulans (formerly oerskovia xanthineolytica) was the principal microorganism isolated from fetal tissues or placentas from cases of equine abortion, premature birth, and term pregnancies. significant pathologic findings included chronic placentitis and pyogranulomatous pneumonia. in addition, microscopic and macroscopic alterations in the allantochorion from 4 of 7 cases of placentitis were similar to those caused by cro ...200415305747
ultraviolet-radiation-resistant isolates revealed cellulose-degrading species of cellulosimicrobium cellulans (uvp1) and bacillus pumilus (uvp4).among extremophiles, microorganisms resistant to ultraviolet radiation (uvr) have been known to produce a variety of metabolites (i.e., extremolytes). we hypothesized that natural microbial flora on elevated land (hills) would reveal a variety of uvr-resistant extremophiles and polyextremophiles with modulated proteins and enzymes that had biotechnological implications. microorganisms cellulosimicrobium cellulans uvp1 and bacillus pumilus uvp4 were isolated and identified using 16s rrna sequenci ...201223586916
molecular detection of cellulosimicrobium cellulans as the etiological agent of a chronic tongue ulcer in a human immunodeficiency virus-positive patient.ulcerations appeared on the tongue of a 48-year-old human immunodeficiency virus-positive man. histological findings of the biopsy specimen and the fact that the patient had resided in louisiana led us to suspect "american histoplasmosis". a new ulcer appeared while the patient was being treated with itraconazole, and the gene for 16s rrna of cellulosimicrobium cellulans was amplified. the lesions healed during treatment with oral penicillin and azithromycin.200516081997
molecular characterisation of a thermoactive beta-1,3-glucanase from oerskovia xanthineolytica.molecular characterisation of a lytic thermoactive beta-1,3-glucanase from oerskovia xanthineolytica ll-g109 has been performed. a molecular mass of 27 195.6 +/- 1.3 da and an isoelectric point of 4.85 were determined by electrospray mass spectrometry and from its titration curve, respectively. its thermoactivity profile shows it to be a heat-stable enzyme with a temperature optimum of 65 degrees c. the secondary structure content of the protein was estimated by circular dichroism to be approx. ...19968814220
(meta)genomic insights into the pathogenome of cellulosimicrobium cellulans.despite having serious clinical manifestations, cellulosimicrobium cellulans remain under-reported with only three genome sequences available at the time of writing. genome sequences of c. cellulans lmg16121, c. cellulans j36 and cellulosimicrobium sp. strain mm were used to determine distribution of pathogenicity islands (pais) across c. cellulans, which revealed 49 potential marker genes with known association to human infections, e.g. fic and vbha toxin-antitoxin system. oligonucleotide compo ...201627151933
tryptophan introduction can change β-glucan binding ability of the carbohydrate-binding module of endo-1,3-β-glucanase.endo-1,3-β-glucanase from cellulosimicrobium cellulans dk-1 has a carbohydrate-binding module (cbm-dk) at the c-terminal side of a catalytic domain. out of the imperfect tandem α-, β-, and γ-repeats in cbm-dk, the α-repeat primarily contributes to β-glucan binding. this unique feature is derived from trp273 in α-repeat, whose corresponding residues in β- and γ-repeats are asp314 and gly358, respectively. in this study, we generated trp-switched mutants, w273a/d314w, d270a/w273a/d314w, w273a/g358 ...201728388361
first description of late recurrence of catheter-associated bacteraemia due to cellulosimicrobium cellulans. 201727289255
structural and binding properties of laminarin revealed by analytical ultracentrifugation and calorimetric of the β-1,3-glucans, laminarin, has been widely used as a substrate for enzymes including endo-1,3-β-glucanase. to obtain quantitative information about the molecular interaction between laminarin and endo-1,3-β-glucanase, the structural properties of laminarin should be determined. the results from pioneering work using analytical ultracentrifugation for carbohydrate analysis showed that laminarin from laminaria digitata predominantly exists as a single-chain species with approximately 5% ...201627267066
bacteremia due to cellulosimicrobium cellulans associated with central catheter for hemodialysis. 201727256248
early-onset neonatal sepsis due to cellulosimicrobium cellulans.cellulosimicrobium cellulans represents a rare human pathogen. infections have been reported in immunocompromised hosts or in patients with an underlying disease. the authors describe a rare case of early-onset neonatal sepsis due to cellulosimicrobium cellulans in an infant without any underlying disease. the infant was successfully treated with vancomycin.201020376528
improvement of shelf life of soymilk using immobilized protease of oerskovia xanthineolytica ncim 2839.protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from oerskovia xanthineolyitca ncim 2839. the maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. o. xanthineolytica was found to produce two proteases-p1 and p2. the proteases were purified using 60 % cold acetone precipitation and deae-cellulose ion exchange chromatography. sds-page revealed molecular weigh ...201628330233
cellulosimicrobium cellulans strain e4-5 enzymatic hydrolysis of curdlan for production of (1 → 3)-linked β-d-glucan order to find an efficient enzymatic tool for curdlan degradation to produce (1 → 3)-linked β-d-glucan oligosaccharides, strain e4-5 (registration number jn089883, genbank) was isolated from seaside soil. the 16s rrna gene sequencing classified it as cellulosimicrobium cellulans. it was the first reported microorganism that succeeded in degrading high-set heated curdlan blocks. the ferments of strain e4-5 also showed good degradation effects on laminaran and alkali-neutralized curdlan. due to ...201526428180
cellulosimicrobium aquatile sp. nov., isolated from panagal reservoir, nalgonda, india.a gram-stain positive, non-spore forming, non-motile, yellow-pigmented, rod shaped and coccoid aerobic strain designated 3bp(t) was isolated from panagal reservoir, at nalgonda, telangana, india. based on 16s rrna gene sequence analysis it was identified that strain 3bp(t) belongs to the class actinobacteria and is closely related to cellulosimicrobium funkei vtt e-072700(t), cellulosimicrobium cellulans lmg16221(t) and cellulosimicrobium terreum kctc 19206(t). the dna-dna relatedness data demon ...201526373417
functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel.cellulosome is a kind of multienzyme complex that displays high activity, selectivity, and stability. here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the cellulosimicrobium cellulans wild-type strain f16 isolated from soil microflora. this multienzyme complex, with excellent catalytic efficiency of kcat 13.2 s(-1) to remove the c-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-daxp), has an outstanding tolerance against organic solvents a ...201526347949
modification in the properties of paper by using cellulase-free xylanase produced from alkalophilic cellulosimicrobium cellulans ckmx1 in biobleaching of wheat straw pulp.alkalophilic cellulosimicrobium cellulans ckmx1 isolated from mushroom compost is an actinomycete that produces industrially important and environmentally safer thermostable cellulase-free xylanase, which is used in the pulp and paper industry as an alternative to the use of toxic chlorinated compounds. strain ckmx1 was previously characterized by metabolic fingerprinting, whole-cell fatty acids methyl ester analysis, and 16s rdna and was found to be c. cellulans ckmx1. crude enzyme (1027.65 u/g ...201526220821
enzymatic hydrolysis of 7-xylosyltaxanes by an extracellular xylosidase from cellulosimicrobium find extracellular biocatalysts that can specifically and efficiently remove the c-7 xylosyl group from 7-xylosyltaxanes.201526026963
bioflocculant production from untreated corn stover using cellulosimicrobium cellulans l804 isolate and its application to harvesting microalgae.microalgae are widely studied for biofuel production. nevertheless, harvesting step of biomass is still a critical challenge. bioflocculants have been applied in numerous applications including the low-cost harvest of microalgae. a major bottleneck for commercial application of bioflocculant is its high production cost. lignocellulosic substrates are abundantly available. hence, the hydrolyzates of rice stover and corn stover have been used as carbon source to produce the bioflocculant in previo ...201526500696
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