| [peptidoglycan type and cell wall polysaccharide composition of cellulomonas cartalyticum and some coryneform organisms (author's transl)]. | cellulomonas cartalyticum was found to contain a peptidoglycan type different from that of the other species of cellulomonas. the diamino acid is lysin instead of ornithine and the interpeptide bridge consists of d-asp-d-ser. the same peptidoglycan type occurs in corynebacterium manihot, brevibacterium liticum and arthrobacter luteus. these non cellulolytic organisms are most likely not closely related with cellulomonas cartalyticum, as indicated by the very different g +c content of their dna, ... | 1978 | 98123 |
| purification and partial characterization of three extracellular cellulases from cellulomonas sp. | three extracellular cellulases have been purified from cultures of cellulomonas. one was found in solution in the cell-free supernatant and two others were found to be bound to the cellulose added as a carbon source. the free enzyme and one of the cellulose-bound enzymes bind to sephadex. the two cellulose-bound enzymes are glycosylated. the three enzymes behave as endocellulases towards soluble carboxymethyl-cellulose and have little activity on cellulose powder. | 1978 | 98328 |
| occurrence of squalene and sterols in cellulomonas dehydrogenans (arnaudi 1942) comb. nov. hester 1971. | the neutral lipid fraction of the photochromogenic, coryneform bacterium cellulomonas dehydrogenans (arnaudi 1942) comb. nov. contains the sterol precursor squalene and at least two sterols, cholesterol and beta-sitosterol. the compounds were characterized by mass spectrometry and combination gas-liquid chromatography--mass spectrometry. de novo sterol biosynthetic ability was shown from incorporation of 14c from d-[u-14c]glucose into squalene and the sterol fraction. the squalene concentration ... | 1978 | 101527 |
| comparative immunological study of catalases in the genus micrococcus. | double immunodiffusion tests were performed with crude extracts from various micrococcus species and antisera against catalase of micrococcus luteus ccm 169. cell-free extracts of m. lylae atcc 27566 exhibited good cross-reaction. cell-free extracts or catalase enriched preparations of m. varians reacted very weakly and no reaction has been found with preparation of m. kristinae, m. nishinomiyaensis, m. roseus and m. sedentarius. the quantitative microcomplement fixation assay also revealed a cl ... | 1977 | 71880 |
| in-vitro cleavage of a fusion protein bound to cellulose using the soluble yscfs (kex2) variant. | in order to show site-specific cleavage of fusion proteins with an engineered soluble yscf variant, we have constructed a fusion gene encoding eglinc from hirudo medicinalis and the cellulose-binding domain from the cellulomonas fimi exoglucanase (cex). the two fusion partners were separated by a lys-arg-containing recognition sequence for the yeast endoprotease yscf (kex2). the fusion protein (eglinc-cex) was expressed intracellularly in saccharomyces cerevisiae. after disruption of the cells e ... | 1992 | 1368916 |
| the gene encoding the cellulase (avicelase) cel1 from streptomyces reticuli and analysis of protein domains. | streptomyces reticuli produces an unusual cellulase (avicelase), with an apparent molecular weight of 82 kda, which is solely sufficient to degrade crystalline cellulose. during cultivation the processing of the avicelase to a truncated enzyme (42 kda) and an inactive protein (40 kda) correlated with the occurrence of an extracellular protease. after its purification this 36 kda protease cleaved the s. reticuli avicelase in vitro in the same manner. using antibodies raised against the avicelase ... | 1992 | 1282194 |
| beta-eliminative cleavage of the acidic polysaccharide of fusarium sp. m7-1 by an enzyme preparation of cellulomonas sp. | by digestion with an enzyme preparation derived from a culture filtrate of cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and beta 1----2 mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of fusarium sp. m7-1 by acetolysis. the unsaturated disaccharide produced was isolated and identified as 4-deoxy-l-threo-hex-4-enopyranouronosyl alpha(1----2)-d-galactose. the same unsaturated sugar linked to the polysa ... | 1990 | 1368507 |
| growth properties of cellulomonas flavigena mutants affected in cellulose utilization. | the role of cellobiose metabolism in cellulose utilization by cellulomonas flavigena was investigated by studying mutants unable to grow on cellobiose or cellulose. the results show that the ability to utilize cellulose is strictly dependent on the ability to utilize cellobiose. | 1978 | 415038 |
| sensitivity of cellulolytic bacteria to antibiotics. | the sensitivity of eight cellulolytic bacterial strains to eight antibiotics was tested. the results showed that, in general, the strains belonging to cytophaga, cellvibrio, and cellfalcicula are more sensitive to antibiotics than those strains that belong to sporocytophaga and cellulomonas. the inhibitory activity of the tested antibiotics, though differing with different strains, showed the following categories: tetracycline, erythromycin, and chloromycetin were most active, kanamycin, strepto ... | 1977 | 414477 |
| gradient-feed method of growing high cell density cultures of cellulomonas in a bench-scale fermentor. | | 1977 | 402958 |
| a method using solubility to predict dry matter digestibility of cellulosic materials. | a rapid chemical procedure based on the solubility of a holocellulose sample in a system of dimethyl sulfoxide with paraformaldehyde has been developed to provide a laboratory method for predicting dry matter digestibility of cellulose containing samples. the amount of dry matter solubilized by the chemical procedure was closely correlated with anaerobic, in vitro, rumen fluid digestion and with digestibility as measured by aerobic cellulomonas, sp. bacteria. the quantity of solvent and dissolvi ... | 1977 | 402957 |
| pleomorphism in cellulomonas acidula. | pleomorphism of cellulomonas acidula in liquid and on solid media is described. growth in liquid medium is characterized initially by the formation of club-shaped rods and later by cocci. on solid media the organism formed irregular branched cells and large swollen cells. | 1979 | 121908 |
| a numerical taxonomic study of coryneform and related bacteria. | two hundred and thirty-three strains of coryneform bacteria, including representatives of the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix, jensenia, kurthia, listeria, microbacterium, mycobacterium, nocardia and propionibacterium and other related bacteria, were studied using 173 morphological, physiological and biochemical tests. the bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 degrees c ... | 1975 | 805825 |
| isoprenoid quinones in the classification of coryneform and related bacteria. | menaquinones were the only isoprenoid quinones found in 85 of the 95 coryneform bacteria examined. dihydromenaquinones having nine isoprene units were the main components isolated from corynebacterium bovis, from other glutamic acid-producing strains, and from arthrobacter globiformis and related species. dihydromenaquinones with eight isoprene units were found in brevibacterium linens, the remaining corynebacterium species and strains probably belonging to the genus rhodococcus. tetrahydromenaq ... | 1979 | 107269 |
| analysis of functional domains of endoglucanases from clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction. | the nucleotide sequence of engd, an endo-beta-1,4-glucanase gene from clostridium cellulovorans was determined (genbank accession no. m37434). the cooh-terminal part of the gene product, engd, contained a thr-thr-pro repeated sequence followed by a region that has homology to the exoglucanase of cellulomonas fimi. engd and engb, another c. cellulovorans endoglucanase, show 75% amino acid sequence homology at their nh2-termini, in contrast to their carboxyterminal domains which show no homology. ... | 1992 | 1538700 |
| thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa. | acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, corynebacterium haemolyticum, corynebacterium pyogenes or from representatives of the genera arthrobacter, cellulomonas, curtobacterium, kurthia or oerskovia. thin-layer chromatography of whole-organism methanolysates ... | 1976 | 825611 |
| purification and characterization of a novel lyase from cellulomonas sp. that degrades fusarium and gibberella acidic polysaccharides. | a cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of fusarium sp. m7-1 with the formation of mannose and o-beta-d-mannopyranosyl-(1----2)-d-mannose. deae-toyopearl 650m column chromatography showed three lyase activity peaks (fractions i, ii, and iii). the major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. the optimum ph was 6.5 to 8.0 and the stable ph range was 6.0 to ... | 1991 | 1368728 |
| macromolecular composition of a cellulomonas sp. cultivated in continuous culture under glucose and zinc limitation. | the mutant strain of cellulomonas sp. (atcc 21399) was cultivated under glucose and zinc limitation at a variety of growth rates in continuous culture. the growth characteristics and macromolecular composition of the population varied with the limitation imposed and the growth rate. glucose- and zinc-limited cultures maintained a constant relative protein content. the relative ribonucleic acid content increased, whereas the carbohydrate and deoxyribonucleic acid contents decreased with an increa ... | 1979 | 114114 |
| the tertiary structure of endo-beta-1,4-glucanase b (cenb), a multidomain cellulase from the bacterium cellulomonas fimi. | endo-beta-1,4-glucanase b (cenb) is a large (110 kda) extracellular enzyme from the cellulolytic bacterium cellulomonas fimi. cenb contains five domains, including a typical c.fimi cellulose-binding domain, separated by distinctive linker polypeptides (meinke et al., 1991b). x-ray scattering analyses show that cenb has a highly elongated shape resembling beads on a string. the sizes of the polypeptides produced by treatment of cenb with proteases, together with their n-terminal amino acid sequen ... | 1992 | 1421753 |
| crystallization and preliminary x-ray diffraction analysis of the catalytic domain of cex, an exo-beta-1,4-glucanase and beta-1,4-xylanase from the bacterium cellulomonas fimi. | single crystals of the catalytic domain of cex, an exo-beta-1,4-glucanase and beta-1,4-xylanase from the cellulolytic bacterium cellulomonas fimi, have been grown in the presence of polyethylene glycol 4000 using the vapour diffusion technique. the crystals, which diffract to better than 2.0 a resolution, belong to space group p4(1)2(1)2 or p4(3)2(1)2 and have cell constants: a = b = 88.21 a, c = 81.10 a; alpha = beta = gamma = 90 degrees. | 1992 | 1453471 |
| sequence analysis of a gene cluster encoding cellulases from clostridium cellulolyticum. | the sequence of a 5633-bp ecori-pvuii dna fragment from clostridium cellulolyticum was determined. this fragment contains two complete endo-beta-1,4-glucanase-encoding genes, designated celccc and celccg. these two genes are flanked by two other partial open reading frames (orf1 and celcce) that probably encode two cellulases or related enzymes. the celccc and celccg genes appear to be present in a polycistronic transcriptional unit. northern blot hybridisations with intragenic probes derived fr ... | 1992 | 1398087 |
| the binding of cellulomonas fimi endoglucanase c (cenc) to cellulose and sephadex is mediated by the n-terminal repeats. | endoglucanase c (cenc) from cellulomonas fimi binds to cellulose and to sephadex. the enzyme has two contiguous 150-amino-acid repeats (n1 and n2) at its n-terminus and two unrelated contiguous 100-amino-acid repeats (c1 and c2) at its c-terminus. polypeptides corresponding to n1, n1n2, c1, and c1c2 were produced by expression of appropriate cenc gene fragments in escherichia coli. n1n2, but not n1 alone, binds to sephadex; both polypeptides bind to avicel, (a heterogeneous cellulose preparation ... | 1992 | 1375311 |
| the adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose. | cena is a bacterial cellulase (beta-1,4-glucanase) comprised of a globular catalytic domain joined to an extended cellulose-binding domain (cbd) by a short linker peptide. the adsorption of cena and its two isolated domains to crystalline cellulose was analyzed. cena and cbd.ptcena' (the cbd plus linker) adsorbed rapidly to cellulose at 30 degrees c, and no net desorption of protein was observed during the following 16.7 h. there was no detectable adsorption of the catalytic domain. scatchard pl ... | 1992 | 1551882 |
| endoglucanase a from cellulomonas fimi in which the hinge sequence of human iga1 is substituted for the linker connecting its two domains is hydrolyzed by iga proteases from neisseria gonorrhoeae. | the hinge in iga1 and the linker in endoglucanase a (cena) are quite similar. the iga1 hinge is 18 amino acids long and contains only proline, threonine and serine. the linker in cena is 27 amino acids long and contains only proline, threonine and a single serine. iga proteases from neisseria gonorrhoeae cleave pro-ser and pro-thr bonds within the iga1 hinge sequence, but they do not attack cena. when the linker sequence of cena is replaced with the hinge sequence of iga1, the hybrid polypeptide ... | 1992 | 1601289 |
| cellobiose chemotaxis by the cellulolytic bacterium cellulomonas gelida. | in the course of a study on the bacterial degradation of plant cell wall polysaccharides, we observed that growing cells of motile cellulolytic bacteria accumulated, without attachment, near cellulose fibers present in the cultures. because it seemed likely that the accumulation was due to chemotactic behavior, we investigated the chemotactic responses of one of the above-mentioned bacteria (cellulomonas gelida atcc 488). we studied primarily the responses toward cellobiose, which is the major p ... | 1992 | 1459948 |
| cloning and sequencing of the cela gene encoding endoglucanase a of butyrivibrio fibrisolvens strain a46. | genomic dna from butyrivibrio fibrisolvens strain a46 was digested with ecori and ligated into lambda gt11. two recombinant phages isolated from the gene bank hydrolysed carboxymethylcellulose and were shown to contain the same 2.3 kb ecori restriction fragment, which was cloned into puc12 to generate pba46. escherichia coli jm83 harbouring pba46 expressed an endoglucanase (ega) which hydrolysed a range of other substrates including barley beta-glucan, avicel, filter paper and p-nitrophenyl beta ... | 1990 | 2269875 |
| streptomyces lividans glycosylates an exoglucanase (cex) from cellulomonas fimi. | exoglucanase cex from cellulomonas fimi is a glycoprotein [langsford et al., j. gen. microbiol. 130 (1984) 1367-1376]. cex produced by streptomyces lividans from the cloned cex gene is also glycosylated. the extent and nature of glycosylation are similar for cex from both organisms. the glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. the ability to glycosylate cloned gene products enhances the utility ... | 1992 | 1427088 |
| cellulose-binding domains: potential for purification of complex proteins. | the endoglucanase cena and the exoglucanase cex from cellulomonas fimi each contain a discrete cellulose-binding domain (cbd), at the amino-terminus or carboxyl-terminus respectively. the gene fragment encoding the cbd can be fused to the gene of a protein of interest. using this approach hybrid proteins can be engineered which bind reversibly to cellulose and exhibit the biological activity of the protein partner. alkaline phosphatase (phoa) from escherichia coli, and a beta-glucosidase (abg) f ... | 1992 | 1409557 |
| enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein. | using molecular genetic techniques, a fusion protein has been produced which contains the cellulose-binding domain (cbd) of an exoglucanase (cex) from cellulomonas fimi fused to a beta-glucosidase (abg) from agrobacterium sp. the cbd functions as an affinity tag for the simultaneous purification and immobilization of the enzyme on cellulose. binding to cellulose was stable for prolonged periods at temperatures from 4 degrees c to at least 50 degrees c, at ionic strengths from 10 mm to greater th ... | 1991 | 1367528 |
| glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from cellulomonas fimi. | in addition to its known substrate activity with p-nitrophenyl beta-cellobioside, the exoglucanase from cellulomonas fimi has been shown to utilize substituted phenyl beta-glucosides as substrates, of which the best is 2',4'-dinitrophenyl beta-d-glucopyranoside. the enzyme can be inactivated by treatment with 2',4'-dinitrophenyl 2-deoxy-2-fluoro-beta-d-glucopyranoside, by trapping of the covalent intermediate in catalysis, as has been shown for a beta-glucosidase (withers, s.g., and street, i.p. ... | 1991 | 1678739 |
| a numerical taxonomic survey of listeria and related bacteria. | a numerical taxonomic study was performed on named strains of listeria, erysipelothrix, microbacterium thermosphactum, lactobacillus, streptococus, propionibacterium, kurthia and some possibly related bacteria using i43 unit characters covering a wide range of properties. the strains fell into six main clusters: (a) listeria; (b) microbacterium thermosphactum, lactobacillus, streptococcus; (c) gemella, erysipelothrix; (d) kurthia and mainly aerobic corynebacteria; (e) propionibacterium, staphylo ... | 1977 | 856940 |
| characterization and comparison of clostridium cellulovorans endoglucanases-xylanases engb and engd hyperexpressed in escherichia coli. | by the use of a t7 expression system, endoglucanases-xylanases engb and engd from clostridium cellulovorans were hyperexpressed and purified from escherichia coli. the two enzymes demonstrated both endoglucanase and xylanase activities. the substrate specificities of both endoglucanases were similar except that engd had four-times-greater p-nitrophenyl beta-1,4-cellobiosidase activity. the two proteins were very homologous (80%) up to the pro-thr-thr region which divided the protein into -nh2- a ... | 1992 | 1735727 |
| characterization and structure of an endoglucanase gene cena of cellulomonas fimi. | two bamhi fragments (0.8 and 5.2 kb) of cellulomonas fimi containing an endoglucanase (eng) gene (cena) were individually cloned into the bamhi site of pbr322; they expressed carboxymethylcellulase activity in escherichia coli. the nucleotide (nt) sequence of the cena gene was determined by sequencing overlapping deletions. the cena gene is 1350 bp long encoding a polypeptide of 449 amino acids (aa) and stop codon. the 0.8-kb bamhi component encodes the first 76 aa, whereas the 5.2-kb bamhi comp ... | 1986 | 3023193 |
| secretion of cellulomonas fimi exoglucanase by escherichia coli. | a leader sequence of 41 amino acids (aa) has been proposed as the signal sequence for the exoglucanase (exg) from cellulomonas fimi. the ability of this 41-aa peptide to function as a leader sequence has been shown here by gene fusion experiments in escherichia coli. a hybrid leader sequence containing c-terminal 37 aa of the leader peptide and n-terminal 6 aa of beta-galactosidase (beta gal) directed export of the exg into the periplasm of e. coli. in contrast, hybrid beta gal-exg proteins in w ... | 1986 | 3023194 |
| derepressed synthesis of cellulase by cellulomonas. | a cellulomonas sp. was isolated from the soil which hydrolyzed cellulose, as shown by clear-zone formation on cellulose agar medium. catabolite repression of cellulase synthesis occurred when moderate levels of glucose were added to the medium. a stable mutant that no longer exhibits catabolite repression was produced through treatment of the wild-type organism with n-methyl-n'-nitro-n-nitrosoguanidine. both enzyme concentration and specific activity, as determined by the rate of hydrolysis of c ... | 1976 | 824282 |
| molecular cloning and nucleotide sequence of the gene encoding an endo-1,4-beta-glucanase from bacillus sp. ksm-330. | the gene encoding an acid endo-1,4-beta-glucanase from bacillus sp. ksm-330 was cloned into the hindiii site of pbr322 and expressed in escherichia coli hb101. the recombinant plasmid contained a 3.1 kb hindiii insert, 1.8 kb of which was sufficient for the expression of endoglucanase activity in e. coli hb101. nucleotide sequencing of this region (1816 bp) revealed an open reading frame of 1389 bp. the protein deduced from this sequence was composed of 463 amino acids with an mr of 51882. the d ... | 1991 | 1770347 |
| microbial fermentation of rice straw: nutritive composition and in vitro digestibility of the fermentation products. | rice straw was fermented with cellulomonas sp. and alcaligenes faecalis. microbial cells and undigested residue, as well as chemically treated (naoh or nh4oh) and untreated straws, were analyzed for nutrient composition and in vitro digestibility. in a typical fermentation, 75% of the rice straw substrate was digested, and 18.6% of the total substrate weight that disappeared was recovered as microbial protein. the microbial cell fraction was 37% protein and 5% crude fiber; the residue was 12% pr ... | 1975 | 804853 |
| structural and functional relationships in two families of beta-1,4-glycanases. | cena and cex are beta-1,4-glycanases produced by the cellulolytic bacterium cellulomonas fimi. both enzymes are composed of two domains and contain six cys residues. two disulfide bonds were assigned in both enzymes by peptide analysis of the isolated catalytic domains. a further disulfide bond was deduced in both cellulose-binding domains from the absence of free thiols under denaturing conditions. corresponding cys residues are conserved in eight of nine other known c. fimi-type cellulose-bind ... | 1991 | 1761039 |
| differentiation of genera of the coryneform bacteria. | a scheme of the generic structure of the group of coryneform bacteria, including the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, and rhodococcus, is suggested. morphological, chemotaxonomic (presence and stereochemical form of diaminopimelic acid, lipid a, and l-arabinose), and physiological features were used as diagnostic criteria. the position of microbacterum and mycococcus and of coryneforms with a nocardial wall but giving a positive hugh-leifson anaerobic test, and ... | 1979 | 121544 |
| purification, properties, and partial amino acid sequences of thermostable xylanases from streptomyces thermoviolaceus opc-520. | two types of xylanases (1,4-beta-d-xylan xylanohydrolase, ec 3.2.1.8) were isolated from the culture filtrate of a thermophilic actinomycete, streptomyces thermoviolaceus opc-520. the enzymes (stx-i and stx-ii) were purified by chromatography with deae-toyopearl 650 m, cm-toyopearl 650 m, sephadex g-75, phenyl-toyopearl 650 m, and mono q hr. the purified enzymes showed single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the molecular weights of stx-i and stx-ii were 54,000 ... | 1992 | 1539982 |
| multiple xylanases of cellulomonas fimi are encoded by distinct genes. | cellulomonas fimi genomic dna encoding xylanase activity has been cloned and expressed in escherichia coli. as judged by dna hybridization and restriction analysis, twelve xylanase-positive clones carried a minimum of four different xylanase (xyn) genes. the encoded enzymes were devoid of cellulase activity but three of the four bound to avicel. | 1991 | 1769538 |
| sequence analysis of the cellulase-encoding cely gene of erwinia chrysanthemi: a possible case of interspecies gene transfer. | the erwinia chrysanthemi (strain 3937) cely gene encoding the minor endoglucanase (egy) was sequenced. the analysis of the upstream region allowed us to identify an in vivo active promoter recognized by the ntra (sigma 54) holoenzyme. no similarity was found between the predicted amino acid (aa) sequences of egy and either the er. chrysanthemi major endoglucanase, egz, or the er. carotovora cels endoglucanase. in contrast, a very high level of identity, both at the nucleotide and the predicted a ... | 1991 | 1937031 |
| multiple domains in endoglucanase b (cenb) from cellulomonas fimi: functions and relatedness to domains in other polypeptides. | endoglucanase b (cenb) from the bacterium cellulomonas fimi is divided into five discrete domains by linker sequences rich in proline and hydroxyamino acids (a. meinke, c. braun, n. r. gilkes, d. g. kilburn, r. c. miller, jr., and r. a. j. warren, j. bacteriol. 173:308-314, 1991). the catalytic domain of 608 amino acids is at the n terminus. the sequence of the first 477 amino acids in the catalytic domain is related to the sequences of cellulases in family e, which includes procaryotic and euca ... | 1991 | 1938913 |
| nucleotide sequence of the endoglucanase c gene (cenc) of cellulomonas fimi, its high-level expression in escherichia coli, and characterization of its products. | the cenc gene of cellulomonas fimi, encoding endoglucanase cenc, has an open reading frame of 1101 codons closely followed by a 9 bp inverted repeat. the predicted amino acid sequence of mature cenc, which is 1069 amino acids long, is very unusual in that it has a 150-amino-acid tandem repeat at the n-terminus and an unrelated 100-amino-acid tandem repeat at the c-terminus. cenc belongs to subfamily e1 of the beta-1,4-glycanases. high-level expression in escherichia coli of cenc from a 3.6 kbp f ... | 1991 | 1956299 |
| immunological versatility and carbon regulation of cellulomonas fimi endo-1,4-beta-glucanases. | more than 10 protein molecules with endo-1,4-beta-glucanase activity were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zymogram in cellulomonas fimi culture supernatants, grown in cmc as carbon source. these molecules are shown to belong to at least four immunologically different groups, against three of which polyclonal antibodies were raised. the protein species used as antigens showed significant differences in cross reactivity, carbon regulation, and affinity t ... | 1991 | 1777121 |
| the tertiary structure of a bacterial cellulase determined by small-angle x-ray-scattering analysis. | cena from cellulomonas fimi is a beta-1,4-endoglucanase that binds tightly to cellulose. x-ray-scattering analyses show that the enzyme is tadpole-shaped: the previously identified catalytic and cellulose-binding domains comprise the head and tail respectively. it appears that this structural and functional organization is common to several cellulases from bacteria and fungi. | 1990 | 2121133 |
| dna sequences of three beta-1,4-endoglucanase genes from thermomonospora fusca. | the dna sequences of the thermomonospora fusca genes encoding cellulases e2 and e5 and the n-terminal end of e4 were determined. each sequence contains an identical 14-bp inverted repeat upstream of the initiation codon. there were no significant homologies between the coding regions of the three genes. the e2 gene is 73% identical to the cela gene from microbispora bispora, but this was the only homology found with other cellulase genes. e2 belongs to a family of cellulases that includes cela f ... | 1991 | 1904434 |
| phylogenetic analysis of the coryneform bacteria by 5s rrna sequences. | nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ... | 1987 | 3106318 |
| purification and characterization of two native extracellular carboxymethylcellulases of cellulomonas flavigena. | | 1990 | 2125947 |
| expression of the cellulomonas fimi cellulase genes cex and cena from the divergent tet promoters of transposon tn10. | a cartridge was constructed which contained the divergent tet promoters of transposon tn10 between an exoglucanase gene (cex) and an endoglucanase gene (cena) of cellulomonas fimi. when carried in a broad-host-range vector, the cartridge gave expression of cex and cena in escherichia coli, rhodobacter capsulatus and klebsiella pneumoniae. | 1990 | 2154164 |
| purification and characterization of three extracellular carboxymethylcellulases of cellulomonas flavigena. | | 1990 | 2125948 |
| dna sequence of a fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase) gene. | the dna sequence of a mixed-linkage beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase [ec 3.2.1.73]) gene from fibrobacter succinogenes cloned in escherichia coli was determined. the general features of this gene are very similar to the consensus features for other gram-negative bacterial genes. the gene product was processed for export in e. coli. there is a high level of sequence homology between the structure of this glucanase and the structure of a mixed-linkage beta-glucanase from ba ... | 1990 | 2193918 |
| regulation and initiation of cenb transcripts of cellulomonas fimi. | we characterized the in vivo transcription of the cellulomonas fimi cenb gene, which encodes an extracellular endo-beta-1,4-glucanase (ec 3.2.1.4). by northern blot (rna blot) analysis, cenb mrna was detected in c. fimi rna preparations from glycerol-, glucose-, and carboxymethyl cellulose (cmc)-grown cells. the relative abundance of the specific mrnas in these preparations appeared to depend on the carbon source provided, with the preparations from cmc-grown cells having the greatest amount of ... | 1987 | 2443484 |
| unusual sequence organization in cenb, an inverting endoglucanase from cellulomonas fimi. | the nucleotide sequence of the cenb gene was determined and used to deduce the amino acid sequence of endoglucanase b (cenb) of cellulomonas fimi. cenb comprises 1,012 amino acids and has a molecular weight of 105,905. the polypeptide is divided by so-called linker sequences rich in proline and hydroxyamino acids into five domains: a catalytic domain of 607 amino acids at the n terminus, followed by three repeats of 98 amino acids each which are greater than 60% identical, and a c-terminal domai ... | 1991 | 1987122 |
| [biochemical taxonomic studies of the genus cellulomonas]. | | 1974 | 4154655 |
| fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose. | endoglucanase cena of cellulomonas fimi comprises an n-terminal cellulose-binding domain and a c-terminal catalytic domain joined together by a sequence of 23 proline and threonine residues (the pro-thr box). the domains function independently when separated by proteolysis. tnphoa has been used to generate cena'-'phoa fusions. cena'-'phoa fusion polypeptides which contain the entire cellulose-binding domain of cena bind to cellulose, allowing their purification from periplasmic extracts in a sin ... | 1989 | 2494059 |
| homologues of catalytic domains of cellulomonas glucanases found in fungal and bacillus glycosidases. | we demonstrate homology between the catalytic domains of exoglucanase (1,4-beta-d-glucan cellobiohydrolase, ec 3.2.1.91) from cellulomonas fimi and those of endoxylanases (1,4-beta-d-xylan xylanohydrolases, ec 3.2.1.8) from bacillus sp. strain c-125 and the fungus cryptococcus albidus; and between the catalytic domains of endoglucanase (1,4-(1,3:1,4)-beta-d-glucan 4-glucanohydrolase, ec 3.2.1.4) from cellulomonas fimi and exoglucanase ii from trichoderma reesei. these five enzymes apparently evo ... | 1989 | 2500377 |
| purification and characterization of endoglucanase c of cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene. | two nonglycosylated endoglucanases which bind to sephadex were purified from culture supernatants of cellulomonas fimi grown on microcrystalline cellulose. their mrs were 120,000 and 130,000. the n-terminal amino acid sequences of the enzymes were identical, suggesting that the enzymes were related. a dna fragment encoding this n-terminal sequence was cloned in escherichia coli. the nucleotide sequence corresponding to the n-terminal amino acid sequence was preceded by a sequence encoding a typi ... | 1989 | 2604391 |
| cellulase families revealed by hydrophobic cluster analysis. | the amino acid sequences of 21 beta-glycanases have been compared by hydrophobic cluster analysis. six families of cellulases have been identified on the basis of primary structure homology: (a) endoglucanases b, c and e of clostridium thermocellum; endoglucanases of erwinia chrysanthemi and bacillus sp.; endoglucanase iii of trichoderma reesei; endoglucanase i of schizophyllum commune; (b) cellobiohydrolase ii of t. reesei; endoglucanases of cellulomonas fimi and streptomyces sp; (c) cellobiohy ... | 1989 | 2806912 |
| structural and functional analysis of a bacterial cellulase by proteolysis. | cena is an endo-beta 1,4-glucanase from the cellulolytic bacterium cellulomonas fimi. it is a bifunctional enzyme comprising an amino-terminal cellulose-binding domain and a carboxyl-terminal catalytic domain joined by a short sequence of prolyl and threonyl residues (the pro-thr box). additional structural and functional information was revealed by a detailed analysis of the products generated by proteolytic cleavage of a nonglycosylated form of cena. an extracellular c. fimi protease attacked ... | 1989 | 2681184 |
| direct 1h n.m.r. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex. | the stereochemical courses of the hydrolyses catalysed by three glycosidases have been determined directly by 1h nmr. the anomeric configuration of the initially formed product was ascertained in each case by observation of the chemical shift and coupling constant of the anomeric proton at the new hemiacetal centre. two of the enzymes investigated, an endo-glucanase and an exo-glucanase are components of the cellulase complex of cellulomonas fimi. the third enzyme is the beta-glucosidase from al ... | 1986 | 3094517 |
| biosynthetic regulation of monobutyrin, an adipocyte-secreted lipid with angiogenic activity. | 1-butyrylglycerol (monobutyrin) is a novel angiogenic compound that is synthesized and secreted during the differentiation of 3t3-f442a preadipocytes into adipocytes. to study the regulation of monobutyrin biosynthesis we have developed an assay utilizing the lgycerol kinase enzyme from cellulomonas to quantitate the levels of this compound in cell-conditioned medium. analysis of several cultured cell types, including tumor cell lines, indicated that monobutyrin production is detectable only fro ... | 1991 | 1885616 |
| structure of the gene encoding the exoglucanase of cellulomonas fimi. | in cellulomonas fimi the cex gene encodes an exoglucanase (exg) involved in the degradation of cellulose. the gene now has been sequenced as part of a 2.58-kb fragment of c. fimi dna. the cex coding region of 1452 bp (484 codons) was identified by comparison of the dna sequence to the n-terminal amino acid (aa) sequence of the exg purified from c. fimi. the exg sequence is preceded by a putative signal peptide of 41 aa, a translational initiation codon, and a sequence resembling a ribosome-bindi ... | 1986 | 3096818 |
| purification and partial characterization of two extracellular endoglucanases from cellulomonas fermentans. | avicelase assay of gel slices after non-denaturing polyacrylamide gel electrophoresis of concentrated supernatants from cellulomonas fermentans revealed four active bands. one of them corresponded to the principal active band on cm-cellulose. among the three others, at least one did not correspond to any active band on cm-cellulose and might reflect the presence of an exoglucanase (ec 3.2.1.91). the active band on cm-cellulose was composed of two endoglucanases (ec 3.2.1.4), called cfa and cfb, ... | 1986 | 3096318 |
| nucleotide sequence of the cellulase gene celf of clostridium thermocellum. | the nucleotide sequence of the celf gene of clostridium thermocellum was determined. the open reading frame extended over 2217 bp. the encoded 739-aa polypeptide, celf, with a mw = 82,015, was an endoglucanase with activity against carboxymethylcellulose. the n terminus showed a typical signal peptide, and a cleavage site after ala-27 was predicted. from residues 28 to 470, the sequence of celf was related to the catalytic domains of type e2 endoglucanases, with a strong homology to the endogluc ... | 1991 | 1805307 |
| multigene families of cellulomonas flavigena encoding endo-beta-1,4-glucanases (cm-cellulases). | multiple genes coding for endo-beta-1,4-glucanases (cm-cellulases) have been isolated from a newly discovered highly cellulolytic strain of cellulomonas flavigena. clones of c. flavigena dna were isolated in escherichia coli and screened for gene expression on cm-cellulose plates staining with congo red. six clones produced cm-cellulase activity as detected in liquid assays, and on activity gels. they fell into three groups within which the sequences cross-hybridised. there were small difference ... | 1988 | 3246355 |
| cloning and nucleotide sequence of a cellulase gene, casa, from an alkalophilic streptomyces strain. | a gene encoding an endo-type semi-alkaline cellulase was cloned from an alkalophilic streptomyces strain in streptomyces lividans, and its nucleotide sequence was determined. downstream from the transcriptional start point, which was determined by high-resolution s1 mapping, an open reading frame of 388 amino acids (aa) was present. the n-terminal amino acid sequence of the mature enzyme determined by an edman degradation procedure suggested that the cellulase had an extraordinarily long leader ... | 1988 | 3410319 |
| effects of end-product inhibition of cellulomonas uda anaerobic growth on cellobiose chemostat culture. | cellulomonas uda was grown anaerobically in a chemostat with 3.33 and 11.41 mm cellobiose in the feed medium at dilution rates varying from 0.017 to 0.29/h. unusual results obtained were analyzed by using curves simulating the steady-state biomass. this unusual behavior could be accounted for by a classical growth model taking end-product inhibition into account. acetate has been identified to be the major inhibitor in the experimental conditions used. parameters calculated from experimental obs ... | 1988 | 3131311 |
| deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase a (cena) of cellulomonas fimi alters its conformation and catalytic activity. | the pro-thr box is a linker of 23 amino acids ((pt)4t(pt)7) connecting the catalytic domain and the cellulose-binding domain (cbd) of endoglucanase a (cena) from the bacterium cellulomonas fimi. deletion of the pro-thr box alters the conformation of cena by changing the relative orientation of the catalytic domain and the cbd. the tertiary structures of the catalytic domain and the cbd appear to be unchanged. the change in conformation reduces the catalytic efficiency of the enzyme and masks one ... | 1991 | 1904063 |
| heterologous hybridization of bacterial dna to the endoglucanases a and b structural genes cela and celb of clostridium thermocellum. | dna from various cellulolytic and non-cellulolytic bacteria was found to hybridize to clostridium thermocellum ncib10682 dna fragments carrying the structural genes cela and celb which code for endoglucanases a and b. homology to cela was detected in agrobacterium rhizogenes, azospirillum brasilense, bacillus subtilis, cellulomonas sp., clostridium stercorarium, erwinia chrysanthemi, pseudomonas solanacearum and streptomyces griseus. homology to celb was detected only in b. subtilis, c. stercora ... | 1985 | 4083831 |
| precise excision of the cellulose binding domains from two cellulomonas fimi cellulases by a homologous protease and the effect on catalysis. | an endo-beta-1,4-glucanase (cena) and an exo-beta-1,4-glucanase (cex) were prepared from escherichia coli expressing recombinant dna of the cellulolytic bacterium cellulomonas fimi. purification was facilitated by the high affinities of these enzymes for cellulose. an extracellular c. fimi protease cleaved both enzymes in vivo in a highly specific manner. the affinity of the parent enzyme for cellulose was contained independently in an amino-terminal fragment (p20) of cena and a carboxyl-termina ... | 1988 | 3134347 |
| regulation, initiation, and termination of the cena and cex transcripts of cellulomonas fimi. | we characterized the in vivo transcripts of two cellulomonas fimi genes, the cena gene, which encodes an extracellular endo-beta-1,4-glucanase (ec 3.2.1.4) and the cex gene, which encodes an extracellular exo-beta-1,4-glucanase (ec 3.2.1.91). by northern blot analysis, cena mrna was detected in c. fimi rna preparations from glycerol- and carboxymethyl cellulose-grown cells but not from glucose-grown cells. in contrast, cex mrna was detected only in the preparations from carboxymethyl cellulose-g ... | 1987 | 3804971 |
| amino acid sequence of the threonine-containing mureins of coryneform bacteria. | in a study of the mureins of coryneform bacteria (arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix), 21 threonine-containing strains were found. in several of the strains the amino acid and amino sugar composition of the murein was muramic acid (mur), glucosamine (glcnh(2)), d-glu, l-lys, l-thr, and ala in a molar ratio of 1:1:1:1:1:4 or 5, and in several other strains it was mur, glcnh(2), d-glu, l-lys, l-thr, ala, and l-ser in a molar ratio of 1:1:1:1:1:3:1. the amin ... | 1973 | 4683671 |
| effect of glucose on vanillic acid oxidation in cellulomonas sp. | | 1974 | 4214732 |
| the expression of cellulomonas fimi cellulase genes in brevibacterium lactofermentum. | the exoglucanase gene (cex) and the endoglucanase a gene (cena) from cellulomonas fimi were subcloned into the escherichia coli/brevibacterium lactofermentum shuttle vector pbk10. both genes were expressed to five to ten times higher levels in b. lactofermentum than in e. coli, probably because these genes were expressed from c. fimi promoters. in b. lactofermentum virtually all of the enzyme activities were in the culture supernatant. this system will facilitate analysis of the expression of th ... | 1987 | 3443308 |
| cellulose fermentation: effect of substrate pretreatment on microbial growth. | the effects of chemical, physical, and enzymatic treatments of rice straw and sugarcane bagasse on the microbial digestibility of cellulose have been investigated. treatment with 4% naoh for 15 min at 100 c increased the digestibility of cellulose from 29.4 to 73%. treatment with 5.2% nh(3) could increase digestibility to 57.0% treatments with sulfuric acid and crude cellulase preparation solubilized cellulose but did not increase the digestibility. grinding or high-pressure cooking of the subst ... | 1974 | 4809907 |
| cellulolytic bacteria associated with sloughing spoilage of california ripe olives. | sloughing spoilage of california ripe olives during processing is characterized by severe softening, skin rupture, and flesh sloughing. it was assumed that cellulolytic activity was responsible for skin rupture and sloughing of flesh, and so a deliberate search was made for cellulolytic bacteria from olives undergoing sloughing spoilage. a bacterium identified as cellulomonas flavigena was highly cellulolytic, attacking filter paper, carboxymethyl cellulose (cmc) gel, and olive tissue. other bac ... | 1973 | 4568890 |
| numerical taxonomy of certain coryneform bacteria. | da silva, g. a. nigel (iowa state university, ames), and john g. holt. numerical taxonomy of certain coryneform bacteria. j. bacteriol. 90:921-927. 1965-an electronic computer was used to sort 32 strains of coryneforms into groups with the tree-sort program. the similarity values obtained in this procedure were then used to construct a dendrogram depicting the phenetic resemblance among the taxa. the results indicated that all the phytopathogens studied were sufficiently distinct from the type s ... | 1965 | 4954898 |
| a bifunctional affinity linker to couple antibodies to cellulose. | we have constructed a fusion protein between the staphylococcal a protein and the cellulose binding domain of an exoglucanase (cex) from cellulomonas fimi that can be directly immobilized on cellulose while retaining its capacity to bind immunoglobulin g molecules. the cellulose domain provides binding that does not interfere with the biological activity of the fusion partner, does not involve hazardous chemicals and the matrix does not need to be chemically activated which reduces its cost. we ... | 1993 | 7764247 |
| isolation and characterization of a cellulose-utilizing bacterium. | a cellulose-decomposing aerobic and mesophilic bacterium has been isolated from soils of sugar cane fields. the terminal dilution method was adapted to isolate a single clone of cellulolytic organism from closely related contaminants. the cultural and physiological characteristics of the isolate were studied, and the organism was identified as a member of the genus cellulomonas. the isolate excreted cellulase into the menstruum, and it hydrolyzed various cellulosic materials producing cellobiose ... | 1968 | 5675505 |
| [studies on the factors influencing the digestion of rice straw by cellulomonas]. | a cellulose-decomposing bacterium has been isolated from soil and identified as a member of the genus cellulomonas. rice straw which was delignified with 1% (w/w) sodium hydroxide, then treated with 60% (v/v) phosphoric acid to increase the swelling capacity, could be used effectively by cellulase of cellulomonas. the effects of other conditions such as ph, nitrogen source, substrate concentration and incubation time on the production of filter paper and endoglucanase activity were studied. maxi ... | 1983 | 6413167 |
| identification key for coryneform bacteria derived by numerical taxonomic studies. | six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ... | 1983 | 6413644 |
| structure of the principal carotenoid pigment of cellulomonas biazotea. | the yellow-pigmented cellulomonas biazotea, atcc 486, contains a mixture of carotenoids. the principal compound is a decapreno carotenoid (c50h72o2) tentatively characterized as 2,2'-bis(4-hydroxy-3-methyl-2-butenyl)-gamma,gamma-carotene on the basis of electronic absorption, infrared, proton magnetic resonance, and mass spectrometries. the carotenoid is presumed to be identical to sarcinaxanthin from sarcina lutea pro-synon. micrococcus luteus and, therefore, is isomeric with decaprenoxanthin f ... | 1980 | 6245065 |
| a phylogenetic analysis of the family dermatophilaceae. | the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ... | 1983 | 6195302 |
| a bifunctional exoglucanase-endoglucanase fusion protein. | a fusion was constructed between the cex gene of cellulomonas fimi, which encodes an exoglucanase, and the cena gene of the same organism, which encodes an endoglucanase. the cex-cena fusion was expressed in escherichia coli to give a fusion protein with both exoglucanase and endoglucanase activities. the fusion protein, unlike the cex and the cena gene products from e. coli, did not bind to microcrystalline cellulose, presumably because it lacked an intact substrate-binding region. the fusion p ... | 1987 | 3128463 |
| sequence conservation and region shuffling in an endoglucanase and an exoglucanase from cellulomonas fimi. | cellulomonas fimi produces an endoglucanase and an exoglucanase which bind strongly to cellulose. each enzyme contains three distinct regions: a short sequence of about 20 amino acids containing only proline and threonine (the pro-thr box); an irregular region, rich in hydroxyamino acids, of low charge density, and which is predicted to have little secondary structure; and an ordered region of higher charge density which contains a potential active site, and which is predicted to have secondary ... | 1986 | 3130625 |
| enzymatic asymmetric synthesis of alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols by arylalkyl acylamidases. | with the novel microbial enzyme, 'arylalkyl acylamidase', optically active alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols have been obtained through enantioselective hydrolysis of their racemic amides and esters. (s)-enantiomers of 1-methylbenzylamine, 1-methyl-3-phenylpropylamine and 1-methyl-3-phenylpropanol of high optical purity (> 94% e.e.) were synthesized with the cells of nocardia erythropolis iam 1440 or cellulomonas fimi aku 671. (r)-enantiomer of 1-methyl-3-phenylprop ... | 1994 | 8000864 |
| the priming effect of glucose in soil sterilized by gamma-radiation and reinoculated with cellulomonas sp. | mineralization of native organic matter and u-14c-glucose was followed by measuring the formation of co2 and its radioactivity in chernozem soil presterilized by gamma-radiation and inoculated with a washed suspension of cellulomonas sp. cells. the introduced bacteria mineralized the soil organic component to a higher extent in variants enriched with glucose. this so-called priming effect of glucose was observed also in the presence of chloramphenicol, inhibiting the growth of the bacteria. the ... | 1980 | 6247255 |
| phylogenetic analysis of the genera cellulomonas, promicromonospora, and jonesia and proposal to exclude the genus jonesia from the family cellulomonadaceae. | the 16s rrna gene sequences of eight cellulomonas species, two promicromonospora species, and jonesia denitrificans were determined, and these sequences were compared with the sequences of about 50 representatives of the arthrobacter line of descent in the order actinomycetales. we found that in spite of its current assignment to the family cellulomonadaceae, j. denitrificans branches outside the radiation of this taxon and cannot be considered a member of it. the two promicromonospora species d ... | 1995 | 7547283 |
| identification of some clinical strains of cdc coryneform group a-3 and a-4 bacteria as cellulomonas species and proposal of cellulomonas hominis sp. nov. for some group a-3 strains. | cdc coryneform group a-3 and a-4 bacteria were defined by hollis and weaver in 1981, but their taxonomic position is still unclear. by using biochemical and chemotaxonomical methods, four clinical strains belonging to cdc coryneform groups a-3 (n = 2) and a-4 (n = 2) were studied and could be assigned to the genus cellulomonas, resulting in the first description of cellulomonas strains isolated from clinical specimens. cdc coryneform group a-3 and a-4 strains were compared with the type strains ... | 1995 | 7559954 |
| free and cell-bound cellulase activity of cellulomonas flavigena. | free beta-1, 4-glucanase activity was measured in the supernatant of cultures of cellulomonas flavigena grown on carboxymethylcellulose or filter paper as the main carbon source. filtration through a series of filter papers resulted in quantitative removal of the enzyme from the supernatant. the glucanase was found to be tightly bound to the paper. cellobiose was produced from the filters containing the enzyme, when incubated at 40 degrees c. after removal of the bacterial cells the paper remnan ... | 1984 | 6426384 |
| glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains. | glycosylated cellulases from cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in escherichia coli from recombinant dna. glycosylation of the enzymes does not significantly affect their kinetic properties, or their stabilities towards heat and ph. however, the glycosylated enzymes are protected from attack by a c. fimi protease when bound to cellulose, while the non-glycosylated enzymes yield active, truncated products with greatly reduced affinity for cellulos ... | 1987 | 3121390 |
| comparison of a fungal (family i) and bacterial (family ii) cellulose-binding domain. | a family ii cellulose-binding domain (cbd) of an exoglucanase/xylanase (cex) from the bacterium cellulomonas fimi was replaced with the family i cbd of cellobiohydrolase i (cbhi) from the fungus trichoderma reesei. expression of the hybrid gene in escherichia coli yielded up to 50 mg of the hybrid protein, cexcbdcbhi, per liter of culture supernatant. the hybrid was purified to homogeneity by affinity chromatography on cellulose. the relative association constants (kr) for the binding of cex, ce ... | 1995 | 7635821 |
| molecular cloning of a cellulomonas fimi cellulose gene in escherichia coli. | a sensitive and simple immunoassay was developed to screen escherichia coli transformed with recombinant dna plasmids carrying a cellulase gene. the assay was used to identify a recombinant dna plasmid carrying at least one cellulase gene from cellulomonas fimi. the enzyme present in extracts of e. coli carrying the plasmid was active in catalysing the hydrolysis of carboxymethylcellulose as indicated by the production of reducing sugars. | 1982 | 7044898 |
| cloning of endoglucanase genes from cellulomonas biazotea into e. coli and s. cerevisiae using shuttle vector yep24. | we constructed a smai genomic library of cellulomonas biazotea dna in e. coli and in the s. cerevisiae shuttle vector, yep 24. three clone were identified that conferred the ability for e. coli or s. cerevisiae transformants to produce carboxymethylcellulase (cmcase). cells transformed with these clones were compared with one another and with nontransformed cells for hyper-production of cmcase. in vivo and in vitro studies indicated that the cmcase genes were fully expressed and the enzyme activ ... | 1994 | 7729760 |
| cellobiohydrolase b, a second exo-cellobiohydrolase from the cellulolytic bacterium cellulomonas fimi. | the gene cbhb from the cellulolytic bacterium cellulomonas fimi encodes a polypeptide of 1090 amino acids. cellobiohydrolase b (cbhb) is 1037 amino acids long, with a calculated molecular mass of 109765 da. the enzyme comprises five domains: an n-terminal catalytic domain of 643 amino acids, three fibronectin type iii repeats of 97 amino acids each, and a c-terminal cellulose-binding domain of 104 amino acids. the catalytic domain belongs to family 48 of glycosyl hydrolases. cbhb has a very low ... | 1995 | 7575482 |
| syntheses and testing of substrates and mechanism-based inactivators for xylanases. | the syntheses of the 2,5- and 3,4-dinitrophenyl beta-xylobiosides by two separate routes are described, as well as the syntheses of the 2,4-dinitrophenyl beta-glycosides of 2-chloro-2-deoxy-xylobiose and 2-deoxy-2-fluoro-xylobiose. both the 3,4- and 2,5-dinitrophenyl beta-xylobiosides proved to be good substrates for the bacillus subtilis xylanase, with kcat/km values of 1.0 and 34.4 mm-1 s-1, respectively. excellent time-dependent inactivation of the exoxylanase/glucanase from cellulomonas fimi ... | 1995 | 7585703 |
| a modular xylanase containing a novel non-catalytic xylan-specific binding domain. | xylanase d (xyld) from cellulomonas fimi contains a c-terminal cellulose-binding domain (cbd) and an internal domain that exhibits 65% sequence identity with the c-terminal cbd. full-length xyld binds to both cellulose and xylan. deletion of the c-terminal cbd from xyld abolishes the capacity of the enzyme to bind to cellulose, although the truncated xylanase retains its xylan-binding properties. a derivative of xyld lacking both the c-terminal cbd and the internal cbd homologue did not bind to ... | 1995 | 7717975 |
| specific adhesion and hydrolysis of cellulose by intact escherichia coli expressing surface anchored cellulase or cellulose binding domains. | the entire cex exoglucanase from cellulomonas fimi and the cex cellulose binding domain (cbdcex) were expressed in escherichia coli as fusions to an lpp-ompa hybrid which had been shown earlier to direct a heterologous protein to the cell surface. both cex and cbdcex were readily localized on the cell surface and could be detected by immunofluorescence microscopy, whole cell elisas and functional assays. in cells expressing the entire cex, about 90% of the total cellobiose hydrolase activity was ... | 1993 | 7763519 |
| identification of derivatized peptides without radiolabels: tandem mass spectrometric localization of the tagged active-site nucleophiles of two cellulases and a beta-glucosidase. | a new method that uses nonradioactive active site-directed enzyme inactivators and high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (hplc-esims/ms) to identify labeled peptides in a proteolytic digest is described. this method relies upon the fragmentation of labeled peptides in a predictable and reproducible manner in the collision cell of a tandem mass spectrometer. the exoglycanase from cellulomonas fimi, endoglucanase c from clostridium thermocellum, an ... | 1995 | 7733452 |