| isoprenoid quinones in the classification of coryneform and related bacteria. | menaquinones were the only isoprenoid quinones found in 85 of the 95 coryneform bacteria examined. dihydromenaquinones having nine isoprene units were the main components isolated from corynebacterium bovis, from other glutamic acid-producing strains, and from arthrobacter globiformis and related species. dihydromenaquinones with eight isoprene units were found in brevibacterium linens, the remaining corynebacterium species and strains probably belonging to the genus rhodococcus. tetrahydromenaq ... | 1979 | 107269 |
| [enzymes of intermediary metabolism in coryneform bacteria]. | enzymes of the intermediate metabolism were studied in ten strains of corynebacterium-like organisms belonging to the genera arthrobacter, brevibacterium, corynebacterium and nocardia. all of these were found to contain enzymes of the glycolytic pathway, and nine strains among ten had dehydrogenases of the pentose phosphate shunt. the activity of enzymes of the citric acid cycle was low: alpha-ketoglutarate dehydrogenase was not found in arthrobacter, corynebacterium, brevibacterium linens and n ... | 1979 | 108525 |
| aflatoxin production by aspergillus parasiticus in a competitive environment. | aspergillus parasiticus nrrl 2999 was grown in the presence of rhizopus nigricans, saccharomyces cerevisiae, acetobacter aceti, or brevibacterium linens and aflatoxin concentration was determined after 3,5,7, and 10 days of incubation at 28c. r. nigricans and s. cerevisiae inhibited growth and aflatoxin production by a. parasiticus. b. linens caused slight inhibition and a. aceti stimulated growth and aflatoxin production by a. parasiticus. | 1977 | 339094 |
| antagonistic effect of coryneform bacteria from red smear cheese against listeria species. | a total of 187 coryneform bacteria were isolated from red smear and screened for inhibitory effects against 16 strains of listeria species. culture filtrates from brevibacterium linens (16 strains), arthrobacter nicotianae (4 strains) and arthrobacter nucleogenes (3 strains) showed clear zones of inhibition. the antagonistic effect was seen against 26 to 87% of 91 listeria strains tested. a. nicotianae and a. nucleogenes were more effective against listeria innocua and listeria ivanovii than aga ... | 1991 | 1909545 |
| phylogenetic analysis of the coryneform bacteria by 5s rrna sequences. | nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ... | 1987 | 3106318 |
| survival of brevibacterium linens during nutrient starvation and intracellular changes. | the present work reports the survival capacity of a strain of brevibacterium linens isolated from a french camembert cheese and the ensuing changes in cell composition. exponentially growing cells were harvested, washed and resuspended with shaking in ph 8.0 buffer at 21 degrees c in the absence of a carbon source. the viability of this strain, assessed with slide cultures, is much less than that of coryneform bacteria isolated from soil samples, even though no cell lysis was detected. intracell ... | 1985 | 3994487 |
| numerical taxonomy of certain coryneform bacteria. | da silva, g. a. nigel (iowa state university, ames), and john g. holt. numerical taxonomy of certain coryneform bacteria. j. bacteriol. 90:921-927. 1965-an electronic computer was used to sort 32 strains of coryneforms into groups with the tree-sort program. the similarity values obtained in this procedure were then used to construct a dendrogram depicting the phenetic resemblance among the taxa. the results indicated that all the phytopathogens studied were sufficiently distinct from the type s ... | 1965 | 4954898 |
| activity and purification of linenscin oc2, an antibacterial substance produced by brevibacterium linens oc2, an orange cheese coryneform bacterium. | an orange cheese coryneform bacterium isolated from the surface of gruyère of comté and identified as brevibacterium linens produces an antimicrobial substance designated linenscin oc2. this compound inhibits gram-positive food-borne pathogens including staphylococcus aureus and listeria monocytogenes but is not active against gram-negative bacteria. linenscin oc2 caused viability loss and lysis of the test organism, listeria innocua. electron microscopy showed that linenscin oc2 induces protopl ... | 1995 | 7646021 |
| isolation and identification of cheese-smear bacteria inhibitory to listeria spp. | a newly developed hydrophobic grid membrane method was used to rapidly screen 105 traditional french cheeses for surface smear microorganisms inhibitory to listeria monocytogenes strain v7. approximately 125,000 colonies comprising a wide variety of bacteria were examined of which less than 0.1% produced visible zones of inhibition. isolates possessing antilisterial activity consisted of various strains of enterococcus faecalis, staphylococcus xylosus, staphylococcus warneri and coryneform bacte ... | 1994 | 8024975 |
| chemotaxonomy and molecular taxonomy of some coryneform clinical isolates. | six reference strains of the genus brevibacterium as well as fifteen clinical isolates tentatively assigned to the genus using conventional biochemical methods, were the subject of chemotaxonomic and dna similarity studies. five of these clinical isolates were assigned either to the genera aureobacterium, mycobacterium, gordona or to rhodococcus on the basis of their dna mol% g+c, mycolic acid, amino-acid, sugar and menaquinone contents. among the ten remaining strains, six were not brevibacteri ... | 1993 | 8518510 |
| a brevibacterium linens prbl1 replicon functional in corynebacterium glutamicum. | brevibacterium linens rbl strain cryptic plasmid prbl1 (8.0 kb) is described. a region involved in prbl1 autonomous replication in corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. this region encodes for a hypothetical 310-amino acid (aa) protein closely related to the replicases of plasmids pxz10142 (c. glutamicum) and pal5000 (mycobacterium fortuitum). the 310-aa protein also shows significant homology to proteins of pcole5-099 (shigella sonn ... | 1996 | 8938050 |
| specificity of an extracellular proteinase from brevibacterium linens atcc 9174 on bovine beta-casein. | the specificity of the extracellular proteinase from brevibacterium linens atcc 9174 on bovine beta-casein was studied. hydrolysis was monitored over time by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) and urea-page. the major ph 4.6-soluble peptides were isolated by high-performance liquid chromatography and identified by n-terminal amino acid sequencing and mass spectrometry. the major sites of hydrolysis were ser-18-ser-19, glu-20-glu-21, gln-56-ser-57, gln-72-asn-73, leu ... | 1997 | 9172371 |
| microbiological composition of raw milk from selected farms in the camembert region of normandy. | raw milk from 27 farms was sampled over 6 months for listerias, salmonellas, yersinia enterocolitica and campylobacters. total bacterial counts and somatic cell counts were measured. lactococci, lactobacilli, dextran-producing leuconostocs, brevibacterium linens, yeasts and moulds, staphylococcus aureus and other micrococcaceae, pseudomonas, coliforms, escherichia coli, enterococci, clostridium perfringens and spores of anaerobic lactate-fermenting bacteria were also counted. pseudomonas (2000 c ... | 1997 | 9246770 |
| antibacterial and hemolytic activities of linenscin oc2, a hydrophobic substance produced by brevibacterium linens oc2. | linenscin oc2 is an antibacterial substance produced by the orange cheese coryneform bacterium brevibacterium linens oc2. it inhibits the growth of gram-positive bacteria but it is inactive against gram-negative bacteria. the intact outer membrane of gram-negative bacteria was shown to be an effective permeability barrier against linenscin oc2. at high dosage the effect of linenscin oc2 was bacteriolytic on listeria innocua. bacteriostasis was observed at low dosage and peptidoglycan biosynthesi ... | 1997 | 9271855 |
| cryptic plasmid pka22 isolated from the naphthalene degrading derivative of rhodococcus rhodochrous ncimb13064. | cryptic plasmids were found in rhodococcus rhodochrous ncimb13064 derivatives which had lost the ability to utilize short-chain 1-chloroalkanes (chain length c3-c10) and had acquired the ability to degrade naphthalene. the reversions of these derivatives to the original phenotype were accompanied by the loss of the cryptic plasmids. the 4969-bp pka22 plasmid was cloned in escherichia coli and sequenced. this plasmid encodes a putative 33,200-da protein which contains motifs typical of theta repl ... | 1997 | 9281496 |
| identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. | coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ... | 1997 | 9039559 |
| nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from brevibacterium linens m18. | linocin m18 is an antilisterial bacteriocin produced by the red smear cheese bacterium brevibacterium linens m18. oligonucleotide probes based on the n-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal dna. the amino acid composition, n-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb bamhi restriction fragment correspond closely to those o ... | 1996 | 8919789 |
| specificity of an extracellular proteinase from brevibacterium linens atcc 9174 on bovine alpha s1-casein. | the specificity of the extracellular proteinase from brevibacterium linens atcc 9174 on bovine alpha s1-casein was studied. hydrolysis was monitored over time by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) and urea-page. the major ph 4.6-soluble peptides were isolated by high-performance liquid chromatography and identified by n-terminal amino acid sequencing and mass spectrometry. the time course of peptide formation indicated that his-8-gln-9, ser-161-gly-162, and either g ... | 1996 | 8593051 |
| human infections caused by brevibacterium casei, formerly cdc groups b-1 and b-3. | forty-one clinical strains of cdc coryneform groups b-1 and b-3 were compared biochemically, by analysis of cell wall sugars, amino acids, and cellular fatty acids, and by dna relatedness to the type strains of brevibacterium casei, brevibacterium epidermidis, and brevibacterium linens. twenty-two strains were shown to be b. casei, while five other strains formed a phenotypically inseparable genomospecies in the same genus. the remaining isolates were genetically heterogeneous, and most are prob ... | 1994 | 8077397 |
| pulsed-field gel electrophoresis analysis of the genome of amino acid producing corynebacteria: chromosome sizes and diversity of restriction patterns. | a large number of species of corynebacteria are known to be amino acid producers, including members of the genera corynebacterium and brevibacterium. pulsed-field gel electrophoresis (pfge) of dna fragments obtained by using endonucleases which recognize at-rich hexanucleotide or octanucleotide sequences produces a discrete pattern of bands useful for fingerprinting and physical mapping of the chromosome. using pacl and swal endonucleases the genome of brevibacterium lactofermentum atcc 13869 (g ... | 1994 | 8000547 |
| isolation and characterization of linocin m18, a bacteriocin produced by brevibacterium linens. | brevibacterium linens m18, isolated from red smear cheese, produces a substance that inhibits the growth of listeria spp. and several coryneform and other gram-positive bacteria. no gram-negative bacteria were inhibited. the substance is heat labile, sensitive to proteolytic enzymes, and stable between ph 3 and 12. high levels of this bacteriocin, named linocin m18, were obtained in the stationary growth phase. linocin m18 was purified by ultrafiltration, ultracentrifugation, and gel filtration ... | 1994 | 7986050 |
| brevibacterium linens pbl33 and rhodococcus rhodochrous prc1 cryptic plasmids replicate in rhodococcus sp. r312 (formerly brevibacterium sp. r312). | the replication of two cryptic plasmids from brevibacterium linens atcc 9174 (pbl33) and rhodococcus rhodochrous atcc 4276 (prc1) was investigated in rhodococcus sp. r312 (formerly brevibacterium sp. r312). the recombinant plasmids psp33 (pbl33 derivative) and pspc1 (prc1 derivative) were found to be suitable for establishing new host-vector systems for rhodococcus sp. r312. they all carry the tn903 neomycin-resistance-encoding gene (aphi). | 1995 | 7867954 |
| transport of aromatic amino acids by brevibacterium linens. | whole metabolizing brevibacterium linens cells were used to study the transport of aromatic amino acids. kinetic results followed the michaelis-menten equation with apparent km values for phenylalanine, tyrosine, and tryptophan of 24, 3.5, and 1.8 microm. transport of these amino acids was optimum at ph 7.5 and 25 degrees c for phenylalanine and ph 8.0 and 35 degrees c for tyrosine and tryptophan. crossed inhibitions were all noncompetitive. the only marked stereospecificity was for the l form o ... | 1983 | 6885717 |
| [transport of phenylalanine and tyrosine in brevibacterium linens: specificity and incorporation into proteins]. | the specificity of phenylalanine and tyrosine carriers was investigated using actively metabolizing cells of brevibacterium linens. the cellular protein synthesis of resting cells was very weakly inhibited, even with high concentrations of chloramphenicol or tetracycline. the nonaromatic amino acids were weak inhibitors for these carriers, while fluorinate analogues of phenylalanine and tyrosine were very potent competitive inhibitors. in practice these analogues cannot be used to replace amino ... | 1984 | 6744119 |
| bacterial growth in artificial capillary spaces. | in experiments on the influence of microscopic capillaries on the growth of escherichia coli, brevibacterium linens, micrococcus flavus and bacillus cereus, a distinct delay in growth was observed. the difference in counts was greatest after 8 h. later a slight equilibration of counts was noted. with b. cereus, only slight or no spore formation was observed under microcapillary conditions. | 1984 | 6437860 |
| electrochemical proton gradient of brevibacterium linens and its relationship to phenylalanine transport. | the proton motive force generated by brevibacterium linens was determined by the accumulation of radiolabelled tetraphenylphosphonium for transmembrane potential (delta psi) and by the accumulation of benzoate ions for the h+ chemical gradient (delta ph). in resting cells at ph 8.0, the delta psi was 172 +/- 10 mv while delta ph was 9 mv. the additions of valinomycin to b. linens in the presence of 100 mm exogenous potassium reduced the delta psi and led to a drastic inhibition of phenylalanine ... | 1984 | 6095716 |
| influence of food microorganisms on staphylococcal growth and enterotoxin production in meat. | forty-four microorganisms were studied for their influence on staphylococcal growth and enterotoxin production. inhibition was found to be more common than stimulation. two types of inhibition were observed: inhibition of staphylococcal growth, and inhibition of enterotoxin formation with no apparent effect on growth. by use of a plate test, 12 of the 44 food microorganisms were found to inhibit staphylococcal growth at 35 c. of the 12, 3 also inhibited growth at 25 c. no significant differences ... | 1966 | 5970822 |
| cell-bound lipase and esterase of brevibacterium linens. | the activities of glycerol ester hydrolase, lipase (ec 3.1.1.3) and carboxylesterase, and esterase (ec 3.1.1.1) were determined for whole cell preparations of brevibacterium linens by using the ph-stat assay. the culture growth liquors were inactive against the three substrates, tributyrin emulsion, triacetin, and methyl butyrate. cells washed in water had less activity than cells washed in 5% nacl; the ratio of activities was close to 1:2 for all strains using tributyrin emulsion as the substra ... | 1974 | 4824883 |
| examination of brevibacterium linens by an electrophoretic zymogram technique. | | 1974 | 4820345 |
| taxonomically significant colour changes in brevibacterium linens probably associated with a carotenoid-like pigment. | | 1973 | 4579436 |
| structural studies of a mannitol teichoic acid from the cell wall of bacterium n.c.t.c. 9742. | degradative and n.m.r.-spectroscopic studies have been carried out on a novel mannitol teichoic acid extracted from the cell wall of bacterium n.c.t.c. 9742, for which the name brevibacterium iodinum has been proposed. the backbone of the polymer is a poly(d-mannitol phosphate) containing 1----6 phosphodiester linkages. in most residues, pyruvic acid is acetal-linked to positions 4 and 5 of the mannitol. about half of the mannitol residues carry a beta-d-glucopyranosyl substituent at position 2. ... | 1985 | 3994675 |
| characterization of brevibacterium linens pigmentation using spectrocolorimetry. | spectrocolorimetry was used for the evaluation of brevibacterium linens pigmentation. a total of 23 strains of brevibacterium linens, from various sources, were plated onto solid agar media and their color coordinates were measured and expressed by the l*a*b* colorimetric system. all the strains were located along a straight line with a hue of 66 degrees (orange color). the effect of light exposure versus storage in the dark was also investigated using this approach. three groups with distinct b ... | 2000 | 10868681 |
| production of methanethiol from methionine by brevibacterium linens cnrz 918. | the conditions under which brevibacterium linens cnrz 918, a strain isolated from the surface smear flora of gruyère de comté cheese, produced methanethiol from methionine were studied. demethiolation was estimated from the methanethiol production capacity of resting cells. methionine was demethiolated mainly during the exponential growth phase of the organism during which time the cells were rod-shaped and had a generation time of 5 h, and the medium became alkaline. at the end of growth (ph 9) ... | 1985 | 3989511 |
| inhibition of candida albicans by methanethiol produced by brevibacterium linens. | brevibacterium linens was screened for antifungal activity against candida albicans using several antibiotic assay methods. the growth of c. albicans was inhibited only when the dual culture assay method was employed and using methionine-supplemented media. results suggest that methanethiol which is produced by b. linens' utilization of methionine is the agent inhibitory to c. albicans' growth. | 1985 | 3906369 |
| characteristics of viable brevibacterium linens cells, methionine and cysteine in milkfat-coated microcapsules. | the potential for microencapsulation of viable brevibacterium linens with methionine or cysteine in milkfat to produce sulphur compounds was examined in this study. more than 80 per cent of b. linens cells were encapsulated and then numbers inside capsules increased about three-fold during 48 h at 26 degrees c under anaerobic conditions before a slow decline. most of micro-organisms (7 x 10(7)/ml) were still viable in the capsules after 15 days. more than 90 per cent of cysteine and methionine w ... | 1985 | 3880486 |
| production of hydrogen sulphide in milkfat-coated microcapsules containing brevibacterium linens and cysteine. | milkfat-coated microcapsules containing brevibacterium linens and cysteine were used to produce hydrogen sulphide, one of cheddar cheese flavour compounds. hydrogen sulphide production was substantially reduced and delayed in the encapsulated system as compared with that of the unencapsulated system. hydrogen sulphide was not produced aerobically whereas substantial amounts of hydrogen sulphide were produced in a nitrogen purged system. the inhibitory effect of the initial aerobic condition disa ... | 1989 | 2585242 |
| identification and flagellation of coryneform bacteria from poultry litter. | thirth coryneform isolates from poultry litter were identified and checked for motility and flagellation. twenty-seven formed a yellow pigment and 3 were orange. twenty-three yellow strains were found to be arthrobacter citreus, although starch was hydrolyzed by these strains. four strains, including the three mainly pale yellow isolates, grew on citrate plus an ammonium salt and were classified as a. aurescens. three orange strains were found to be brevibacterium linens. only six strains of a. ... | 1975 | 811345 |
| aminopeptidase from brevibacterium linens: activation and inhibition. | activation and inhibition of a purified aminopeptidase from brevibacterium linens was investigated using l-alpha-leucyl-4-nitroanilide and l-leucyl-l-leucine as substrates. the enzyme was activated by cobalt, provided that the enzyme was preincubated with the metal. strong inhibitory effects were derived from heavy metals, metal-complexing compounds, reducing agents, the modification of aromatic amino acids, and the presence of hydrophobic substances or certain amino acids in the test mixtures. ... | 1978 | 676522 |
| methanethiol production by coryneform bacteria: strains from dairy and human skin sources and brevibacterium linens. | | 1977 | 411888 |
| [distribution of pyrimidine blocks in the dna of brevibacterium linens, arthrobacter globiformis, nocardia corallina and nocardia rubra]. | the nucleotide composition and the frequency of pyrimidine blocks were studied in dna of the following bacteria: brevibacterium linens (weignamm, 1910) breed, 1953; arthrobacter globiformis (conn, 1928) conn et dimmick, 1947; nocardia corallina (bergey et al., 1923) waksman et henrici, 1948; nocardia rubra (krassilnikov, 1949) waksman et henrici, 1948. these organisms are classed by some microbiologists as mycobacteria (the mycobacteriaceae family) while other authors regard them as representati ... | 1975 | 241002 |
| amino acid catabolism in cheese-related bacteria: selection and study of the effects of ph, temperature and nacl by quadratic response surface methodology. | to screen the cystathionine lyase and l-methionine aminotransferase activities of cheese-related bacteria (lactococci, non-starter lactobacilli and smear bacteria) and to determine the individual and interactive effects of temperature, ph and nacl concentration on selected enzyme activities. | 2001 | 11473596 |
| tryptophan catabolism in brevibacterium linens as a potential cheese flavor adjunct. | attempts to develop a desirable reduced fat cheddar cheese are impeded by a propensity for flavor defects such as meaty-brothy, putrid, fecal, and unclean off-flavors in these products. recent studies suggest aromatic amino acid catabolism of starter, adjunct, and nonstarter lactic acid bacteria significantly impact off-flavor development. the objective of this study was to delineate pathways for catabolism of tryptophan (trp) in brevibacterium linens, a cheese flavor adjunct, and to determine t ... | 2001 | 11518299 |
| growth reduction of listeria spp. caused by undefined industrial red smear cheese cultures and bacteriocin-producing brevibacterium lines as evaluated in situ on soft cheese. | the undefined microbial floras derived from the surface of ripe cheese which are used for the ripening of commercial red smear cheeses have a strong impact on the growth of listeria spp. in some cases, these microbial consortia inhibit listeria almost completely. from such undefined industrial cheese-ripening floras, linocin m18-producing (lin+) (n. valdés-stauber and s. scherer, appl. environ. microbiol. 60:3809-3814, 1994) and -nonproducing brevibacterium linens strains were isolated and used ... | 1997 | 9406400 |
| histamine and tyramine degradation by food fermenting microorganisms. | microorganisms suitable for food fermentation were examined with regard to their potential to degrade histamine and tyramine. out of 64 lactic acid bacteria evaluated in this study, 27 degraded histamine and one tyramine, respectively, with low activity. among 32 strains of brevibacterium linens and coryneform bacteria, 21 exhibited histamine and tyramine oxidase activity. none of 20 strains of staphylococcus carnosus tested degraded histamine or tyramine. one strain out of nine strains of geotr ... | 1998 | 9562873 |
| identification and characterization of a 29-kilodalton protein from mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells. | culture filtrate proteins from mycobacterium tuberculosis induce protective immunity in various animal models of tuberculosis. two molecular mass regions (6 to 10 kda and 24 to 36 kda) of short-term culture filtrate are preferentially recognized by th1 cells in animal models as well as by patients with minimal disease. in the present study, the 24- to 36-kda region has been studied, and the t-cell reactivity has been mapped in detail. monoclonal antibodies were generated, and one monoclonal anti ... | 1998 | 9596740 |
| characterization of an antibacterial peptide produced by brevibacterium linens. | the aim of this research was to investigate the antimicrobial activity produced by brevibacterium linens atcc 9175. | 2002 | 11849329 |
| degradation of histamine and tyramine by brevibacterium linens during surface ripening of munster cheese. | red smear formation during fermentation of munster cheese was started by using three different strains of brevibacterium linens as surface inocula. the cheeses were produced with and without supplementation of histamine and tyramine. after smearing the cheese surface for the first time with b. linens viable counts of 10(7) cfu/g were detected. at the end of the logarithmic growth phase cell numbers increased to 10(10) cfu/g and remained constant during the whole ripening period. during a 4-week ... | 1998 | 9678172 |
| intraspecific diversity of brevibacterium linens, corynebacterium glutamicum and rhodococcus erythropolis based on partial 16s rdna sequence analysis and fourier-transform infrared (ft-ir) spectroscopy. | the intraspecific diversity of 31 strains of brevibacterium linens, 27 strains of corynebacterium glutamicum and 29 strains of rhodococcus erythropolis was determined by partial 16s rdna sequence analysis and fourier-transform infrared (ft-ir) spectroscopy. as a prerequisite for the analyses, 27 strains derived from culture collections which had carried invalid or wrong species designations were reclassified in accordance with polyphasic taxonomical data. ft-ir spectroscopy proved to be a rapid ... | 2002 | 11988527 |
| conversion of methionine to thiols by lactococci, lactobacilli, and brevibacteria | methanethiol has been strongly associated with desirable cheddar cheese flavor and can be formed from the degradation of methionine (met) via a number of microbial enzymes. methionine gamma-lyase is thought to play a major role in the catabolism of met and generation of methanethiol in several species of bacteria. other enzymes that have been reported to be capable of producing methanethiol from met in lactic acid bacteria include cystathionine beta-lyase and cystathionine gamma-lyase. the objec ... | 1998 | 9726877 |
| purification and characterization of l-methionine gamma-lyase from brevibacterium linens bl2 | l-methionine gamma-lyase (ec 4.4.1.11) was purified to homogeneity from brevibacterium linens bl2, a coryneform bacterium which has been used successfully as an adjunct bacterium to improve the flavor of cheddar cheese. the enzyme catalyzes the alpha,gamma elimination of methionine to produce methanethiol, alpha-ketobutyrate, and ammonia. it is a pyridoxal phosphate-dependent enzyme, with a native molecular mass of approximately 170 kda, consisting of four identical subunits of 43 kda each. the ... | 1998 | 9726878 |
| mode of action of linenscin oc2 against listeria innocua. | linenscin oc2 is a small hydrophobic substance produced by the orange cheese coryneform bacterium brevibacterium linens oc2. linenscin oc2 inhibits growth of gram-negative bacteria with an altered outer membrane permeability and gram-positive bacteria. it is also able to lyse eucaryotic cells. the mode of action of linenscin oc2 on the listeria innocua cytoplasmic membrane and the effects of environmental parameters were investigated. addition of low doses of linenscin oc2 resulted in an immedia ... | 1998 | 9726891 |
| carnitine acts as a compatible solute in brevibacterium linens. | carnitine is a trimethyl amino acid found at relatively high concentrations in materials of animal origin. exogenously provided l-carnitine was found to stimulate growth of brevibacterium linens atcc 19391 in media with inhibitory osmotic strength. its osmoprotective ability was as potent as that of glycine betaine. electrophoretic and spectroscopic (nmr) analysis showed that this compound is only transiently accumulated, but in significant amounts, by b. linens under hyperosmotic stress and is ... | 1998 | 9766223 |
| pbla8, from brevibacterium linens, belongs to a gram-positive subfamily of cole2-related plasmids. | a 3.1 kb dna fragment from pbla8, a brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. this fragment is characterized by the presence of an ori site located upstream of an operon encoding two proteins, repa and repb, both essential for replication. based on structural similarities and a strong conservat ... | 1998 | 9802024 |
| allergic alveolitis among agricultural workers in eastern poland: a study of twenty cases. | the aim of this study was to identify the specific agents which caused extrinsic allergic alveolitis (eaa) in the selected group of 20 agricultural workers from eastern poland. the microbiological analysis of the samples of plant materials or dusts reported by the patients as causing symptoms has been carried out, followed by allergological tests (inhalation challenge, agar-gel precipitation test, inhibition of leukocyte migration, skin test) with extrinsic microbial antigens. it was found that ... | 1998 | 9852490 |
| homomultimeric protease in the hyperthermophilic bacterium thermotoga maritima has structural and amino acid sequence homology to bacteriocins in mesophilic bacteria. | a novel homomultimeric protease (> 669 kda), based on 31 kda subunits, was purified from cell extracts of the hyperthermophilic bacterium thermotoga maritima. this protease exhibits activity toward chymotrypsin and trypsin substrates, optimally at 90 degrees c and ph 7.1, and has a half-life of 36 min at 95 degrees c. transmission electron microscopy established that the protease consists of a large globular assembly which appears circular from the front view. the function of this protease in t. ... | 1998 | 9872409 |
| structure and organization of the rrnd operon of 'brevibacterium lactofermentum': analysis of the 16s rrna gene. | five rrna operons (rrn) were found by hybridization in the genome of 'brevibacterium lactofermentum' atcc 13869 and corynebacterium glutamicum atcc 13032. 'b. lactofermentum' dsm 20412 differed from the other corynebacteria tested in showing six hybridizing bamhi bands. two of the rrn operons (rrnd and rrne) were located in a single cosmid. sequencing of the rrnd operon showed that it contains a complete 16s rrna-23s rna-5s rrna gene cluster. phylogenetic studies using the complete 16s rrna sequ ... | 1999 | 10220171 |
| a gene (tmpa) for an efflux protein of the transporter family iii from brevibacterium linens oc2, an antibacterial substance-producing strain. | a gene (tmpa) encoding a putative transmembrane protein has been cloned from b. linens oc2, an antibacterial substance-producing strain. the deduced tmpa protein sequence shares similarities to members of the transporter family iii exploiting the transmembrane proton gradient to provide export of toxic compounds such as antiseptics or antibiotics. northern blot analysis indicated that tmpa gene is expressed. length of rna messenger and overlapping of orfs upstream tmpa gene suggested that it mig ... | 1999 | 10341071 |
| aspects of enzymology and biochemical properties of brevibacterium linens relevant to cheese ripening: a review. | brevibacterium linens is a major surface microorganism that is present in the smear of surface-ripened cheeses. the enzymology and biochemical characteristics of b. linens influence the ripening and final characteristics of smear surface-ripened cheeses. proteolytic, peptidolytic, esterolytic, and lipolytic activities, which are of particular importance in the ripening process, are discussed in detail. this review also describes the production of volatile compounds, especially sulfur-containing ... | 1999 | 10342227 |
| inhibition of clostridium botulinum by culture filtrates of brevibacterium linens. | | 1959 | 13829187 |
| a new carboxylesterase from brevibacterium linens ifo 12171 responsible for the conversion of 1,4-butanediol diacrylate to 4-hydroxybutyl acrylate: purification, characterization, gene cloning, and gene expression in escherichia coli. | a carboxylesterase that is responsible for conversion of 1,4-butanediol diacrylate (bda) to 4-hydroxybutyl acrylate (4hba) was found in brevibacterium lines ifo 12171, and purified to homogeneity. the purified enzyme was active toward a variety of diesters of ethylene glycol, 1,4-butanediol, and 1,6-hexanediol. the k(m) and kcat of the enzyme for bda were 3.04 mm and 203,000 s-1, respectively. the reaction with the purified enzyme gave 98 mm 4hba from 100 mm bda for 60 min. the enzyme gene was c ... | 1999 | 10361681 |
| a carotenogenic gene cluster from brevibacterium linens with novel lycopene cyclase genes involved in the synthesis of aromatic carotenoids. | the carotenogenic (crt) gene cluster from brevibacterium linens, a member of the commercially important group of coryneform bacteria, was cloned and identified. an expression library of b. linens genes was constructed and a fragment of the crt cluster was obtained by functional complementation of a colourless b. flavum mutant, screening transformed cells for production of a yellow pigment. subsequent screening of a cosmid library resulted in the cloning of the whole crt cluster from b. linens. a ... | 2000 | 10821176 |
| [three new species of brevibacteria--brevibacterium antiquum sp. nov., brevibacterium aurantiacum sp. nov. and brevibacterium permense sp. nov]. | this work deals with the taxonomic study of 12 orange-pigmented bacteria isolated from permafrost sediments, rice plots, and soils contaminated with wastes from the chemical and salt industries, which were assigned to the genus brevibacterium on the basis of phenotypic characteristics, as well as of some strains described previously as brevibacterium linens. the study revealed three genomic species, whose members and the type strains of the closest species of brevibacterium had dna similarity le ... | 2004 | 15198034 |
| genetic fingerprinting of brevibacterium linens by pulsed-field gel electrophoresis and ribotyping. | members of brevibacterium linens display physiological features that are relevant for cheese production. the genomes of five b. linens strains deposited on culture collections were compared by examining large restriction fragments on pulsed-field gel electrophoresis and detection of polymorphism at the level of 16s rrna genes. pulsed-field analysis with the endonucleases drai and asni showed a characteristic restriction profile for each strain and allowed the calculation of genome sizes ranging ... | 2000 | 10919399 |
| propionicin sm1, a bacteriocin from propionibacterium jensenii df1: isolation and characterization of the protein and its gene. | we purified a bacteriocin from the cell-free supernatant of propionibacterium jensenii df1 isolated from swiss raw milk, and named it propionicin sm1. the heat-stable protein was strongly bactericidal against p. jensenii dsm20274. on the basis of the n-terminal amino acid sequence of the purified protein, a degenerate oligonucleotide probe was designed to locate and clone the corresponding gene of p. jensenii df1. it hybridized exclusively with the df1l-resident plasmid plme106, but not with chr ... | 2000 | 10930068 |
| behavior of brevibacterium linens and debaryomyces hansenii as ripening flora in controlled production of smear soft cheese from reconstituted milk: growth and substrate consumption dairy foods. | experimental cheeses inoculated with debaryomyces hansenii and brevibacterium linens were ripened for 76 d under aseptic conditions. triplicate cheese-making trials were similar as a result of efficient control of the atmosphere. in all trials, d. hansenii grew rapidly during the first 2 d and then slowed, but growth remained exponential until d 10 (generation time around 70 h). total cell counts were higher than the number of viable cells, and after 10 d they remained around 3 x 10(9) yeast/g o ... | 2000 | 10984141 |
| behavior of brevibacterium linens and debaryomyces hansenii as ripening flora in controlled production of soft smear cheese from reconstituted milk: protein degradation. | model smear soft cheeses, prepared with debaryomyces hansenii and brevibacterium linens as ripening starters, were ripened under aseptic conditions. results of the cheese-making trials, in triplicate, were similar and showed similar patterns of protein degradation. in all of the trials, the acid-soluble nitrogen and nonprotein nitrogen (npn) indexes and nh3 concentrations of the rind were low until d 10. the acid-soluble nitrogen and npn of the rind then increased to 100 and 18% of total nitroge ... | 2000 | 10984142 |
| diversity of l-methionine catabolism pathways in cheese-ripening bacteria. | enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. l-methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (cfes) of all the bacteria tested. no l-methionine deaminase activity could be detected in any of the ripening bacteria and l-methionine aminotransferase was detected in cfes of brevibacterium ... | 2000 | 11097940 |
| a single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in phycomyces. | previous complementation and mapping of mutations that change the usual yellow color of the zygomycete phycomyces blakesleeanus to white or red led to the definition of two structural genes for carotene biosynthesis. we have cloned one of these genes, carra, by taking advantage of its close linkage to the other, carb, responsible for phytoene dehydrogenase. the sequences of the wild type and six mutants have been established, compared with sequences in other organisms, and correlated with the mu ... | 2001 | 11172012 |
| assessment of the coloring strength of brevibacterium linens strains: spectrocolorimetry versus total carotenoid extraction/quantification. | brevibacterium linens is a major component of red-smear cheese microflora and imparts color to the cheese rind. the present study was done to evaluate carotenoid contents in 29 strains of this bacterium and to relate the color of the strains to the carotenoids present. a spectrocolorimeter was used to determine the color, and the carotenoid contents were determined by spectrophotorimetric measurement at 450 nm. regression analysis was carried out on the color values a*, b*, and c* to determine w ... | 2001 | 11233019 |
| identification and functional analysis of the gene encoding methionine-gamma-lyase in brevibacterium linens. | the enzymatic degradation of l-methionine and subsequent formation of volatile sulfur compounds (vscs) is believed to be essential for flavor development in cheese. l-methionine-gamma-lyase (mgl) can convert l-methionine to methanethiol (mtl), alpha-ketobutyrate, and ammonia. the mgl gene encoding mgl was cloned from the type strain brevibacterium linens atcc 9175 known to produce copious amounts of mtl and related vscs. the disruption of the mgl gene, achieved in strain atcc 9175, resulted in a ... | 2004 | 15574935 |
| aroma compound production in cheese curd by coculturing with selected yeast and bacteria. | the microorganisms involved in cheese ripening produce various volatile compounds and induce typical flavors that contribute to cheese variety. to investigate aroma compound generation of cheese microflora, we used a dynamic headspace-gas chromatography-mass spectrometry analysis. to obtain good sensitivity and repeatability of quantification, dynamic headspace conditions and sample preparation were first optimized and led to an extraction set up in which samples were heated at 60 degrees c and ... | 2001 | 11699442 |
| biodiversity of the bacterial flora on the surface of a smear cheese. | the bacteria on the surface of a farmhouse smear-ripened cheese at four stages of ripening (4, 16, 23, and 37 days) from inoculated (i.e., deliberately inoculated with brevibacterium linens bl2) and noninoculated (not deliberately inoculated with b. linens bl2) cheese were investigated. the results show that, contrary to accepted belief, b. linens is not a significant member of the surface flora of smear cheese and no microbial succession of species occurred during the ripening of the cheeses. o ... | 2002 | 11823224 |
| l-methionine degradation potentialities of cheese-ripening microorganisms. | volatile sulphur compounds are major flavouring compounds in many traditional fermented foods including cheeses. these compounds are products of the catabolism of l-methionine by cheese-ripening microorganisms. the diversity of l-methionine degradation by such microorganisms, however, remains to be characterized. the objective of this work was to compare the capacities to produce volatile sulphur compounds by five yeasts, geotrichum candidum, yarrowia lipolytica, kluyveromyces lactis, debaryomyc ... | 2001 | 11928962 |
| optimization of headspace solid-phase microextraction (spme) for the odor analysis of surface-ripened cheese. | fifty volatile compounds of surface smear-ripened cheese were detected and identified using headspace solid-phase microextraction (hs-spme) and vacuum distillation coupled to gas chromatography-mass spectrometry. changes in the headspace of aroma compounds were monitored over the whole packaging period (47 days) using the hs-spme method. initially, the concentration of methanethiol increased before reaching a plateau. this evolution could be linked to the growth of brevibacterium linens. during ... | 2002 | 12059164 |
| ecology of mixed biofilms subjected daily to a chlorinated alkaline solution: spatial distribution of bacterial species suggests a protective effect of one species to another. | three bacterial strains (kocuria sp. c714.1, brevibacterium linens b337.1 and staphylococcus sciuri ccl101) were grown together on stainless steel and were subjected daily to a commercial alkaline chlorine solution (22 mg l-1 of free chlorine, ph 11) over a period of 4 weeks. after the daily chemical shock, culture madia [1:20 dilution of tryptic soy broth (tsb-ye/20) or diluted whey] was deposited on the biofilms. the chemical shocks led first to a drop in the culturable population, followed by ... | 2003 | 12542714 |
| complete nucleotide sequence of a native plasmid from brevibacterium linens. | brevibacterium linens has commercial significance in the dairy industry and potential application in the production of bacteriocins and carotenoids. strain development of these industrially significant organisms would be facilitated by the use of vectors, yet few are available. in this study we report the isolation of four novel plasmids from the gram-positive coryneform b. linens, and determine the first complete nucleotide sequence of a native plasmid of b. linens. the cryptic plasmid plim is ... | 2003 | 12726769 |
| evidence for distinct l-methionine catabolic pathways in the yeast geotrichum candidum and the bacterium brevibacterium linens. | tracing experiments were carried out to identify volatile and nonvolatile l-methionine degradation intermediates and end products in the yeast geotrichum candidum and in the bacterium brevibacterium linens, both of which are present in the surface flora of certain soft cheeses and contribute to the ripening reactions. since the acid-sensitive bacterium b. linens is known to produce larger amounts and a greater variety of volatile sulfur compounds (vscs) than the yeast g. candidum produces, we ex ... | 2006 | 16517666 |
| staphylococcus equorum subsp. linens, subsp. nov., a starter culture component for surface ripened semi-hard cheeses. | two staphylococcal strains, rp29t and rp33, were isolated from the main microflora of a surface ripened swiss mountain cheese made from raw milk. these two strains were differentiated from the most closely related species staphylococcus equorum on the basis of dna-dna hybridisation and phenotypic characteristics and are proposed as staphylococcus equorum subsp. linens subsp. nov. they could be distinguished phenotypically from s. equorum by their sensitivity to all 14 tested antibiotics, especia ... | 2003 | 12747407 |
| high cell density cultivation of brevibacterium linens and formation of proteinases and lipase. | brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. b. linens atcc 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed of nutrients. the maximal activities of l-leucine aminopeptidase and cell-associated proteinase were 286 u l-1 and 202 u l-1, respectively. the cell-associated lipolytic activity e ... | 2003 | 12882170 |
| influence of growth conditions on bacteriocin production by brevibacterium linens. | the influence of temperature, nacl concentration and cheese whey media on growth of brevibacterium linens atcc 9175 and production of bacteriocin-like antimicrobial activity was studied. bacteriocin production and activity were higher at 25 degrees c than at 30 degrees c. no significant growth or production of bacteriocins was observed at 37 degrees c. when bacteriocin production was investigated in media containing different concentrations of nacl, increased activity was observed in media conta ... | 2003 | 12883862 |
| the nutritional equivalence of pantothenate and p-aminobenzoate for the growth of bacterium linens. | | 1953 | 13108856 |
| transport capabilities of eleven gram-positive bacteria: comparative genomic analyses. | the genomes of eleven gram-positive bacteria that are important for human health and the food industry, nine low g+c lactic acid bacteria and two high g+c gram-positive organisms, were analyzed for their complement of genes encoding transport proteins. thirteen to 18% of their genes encode transport proteins, larger percentages than observed for most other bacteria. all of these bacteria possess channel proteins, some of which probably function to relieve osmotic stress. amino acid uptake system ... | 2007 | 17490609 |
| production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses. | twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ... | 2007 | 17701035 |
| the influence of various metabolites upon the p-aminobenzoate-pantothenate interaction in bacterium linens. | | 1953 | 13108857 |
| brevibacterium oceani sp. nov., isolated from deep-sea sediment of the chagos trench, indian ocean. | two bacterial strains, designated bbh5 and bbh7(t), were isolated from a deep-sea sediment sample collected from the chagos trench of the indian ocean (1 degrees 06' s 7 degrees 31' e). based on their 16s rrna gene sequence similarity (99.9%), level of dna-dna relatedness (93%) and a number of similar phenotypic characteristics, the two strains are identified as representing the same species. their phylogenetically nearest neighbours, based on 16s rrna gene sequence similarity values (97.9-98.4% ... | 2008 | 18175683 |
| the role of p-aminobenzoate in pantoate synthesis by bacterium linens. | | 1954 | 13152079 |
| taxonomically significant color reactions of brevibacterium linens. | grecz, nicholas (university of chicago, chicago, ill.), and gail m. dack. taxonomically significant color reactions of brevibacterium linens. j. bacteriol. 82:241-246. 1961.-brevibacterium linens was observed to give characteristic color reactions with certain bases and acids. an intensive carmine-red color appeared immediately after addition of a drop of 5 n sodium hydroxide, 5 n potassium hydroxide, and saturated barium hydroxide. a light carmine-red was given by lithium hydroxide, and a light ... | 1961 | 13708147 |
| application of pcr-sscp for molecular epidemiological studies on the exposure of farm children to bacteria in environmental dust. | the environmental exposure of farm children to microorganisms in dust has become a focus of interest, since microbial exposure on farms has been related to a reduced prevalence of asthma and atopic diseases in children. previous studies almost exclusively focused on the determination of microbial counts using conventional culturing or the determination of microbial compounds i.e. endotoxins. in this study pcr-sscp (single-strand conformation polymorphism) was modified for characterising bacteria ... | 2008 | 18321601 |
| catabolism of volatile sulfur compounds precursors by brevibacterium linens and geotrichum candidum, two microorganisms of the cheese ecosystem. | two brevibacterium linens strains and the cheese-ripening yeast geotrichum candidum were compared with regard to their ability to produce volatile sulfur compounds (vscs) from three different precursors namely l-methionine, 4-methylthio-2-oxobutyric acid (kmba) and 4-methylthio-2-hydroxybutyric acid (hmba). all microorganisms were able to convert these precursors to vscs. however, although all were able to produce vscs from l-methionine, only g. candidum accumulated kmba when cultivated on this ... | 2003 | 14580796 |
| identification of aerobic gut bacteria from the kala azar vector, phlebotomus argentipes: a platform for potential paratransgenic manipulation of sand flies. | visceral leishmaniasis is an understudied parasitic disease responsible for significant global morbidity and mortality. we are presently investigating a method of disease prevention termed paratransgenesis. in this approach, symbiotic or commensal bacteria are transformed to produce anti-leishmania molecules. the transformed bacteria are delivered back to sand flies to inactivate the parasite within the vector itself. in this study, we identified 28 distinct gut microorganisms from phlebotomus a ... | 2008 | 19052297 |
| mur-lh, the broad-spectrum endolysin of lactobacillus helveticus temperate bacteriophage phi-0303. | phi-0303 is a temperate bacteriophage isolated from lactobacillus helveticus cnrz 303 strain after mitomycin c induction. in this work, the gene coding for a lytic protein of this bacteriophage was cloned using a library of phi-0303 in escherichia coli dh5alpha. the lytic activity was detected by its expression, using whole cells of the sensitive strain l. helveticus cnrz 892 as the substrate. the lysin gene was within a 4.1-kb dna fragment of phi-0303 containing six open reading frames (orfs) a ... | 2004 | 14711630 |
| heterologous production of methionine-gamma-lyase from brevibacterium linens in lactococcus lactis and formation of volatile sulfur compounds. | the conversion of methionine to volatile sulfur compounds (vscs) is of great importance in flavor formation during cheese ripening and is the focus of biotechnological approaches toward flavor improvement. a synthetic mgl gene encoding methionine-gamma-lyase (mgl) from brevibacterium linens bl2 was cloned into a lactococcus lactis expression plasmid under the control of the nisin-inducible promoter pnisa. when expressed in l. lactis and purified as a recombinant protein, mgl was shown to degrade ... | 2009 | 19251895 |
| cell wall teichoic acids of two brevibacterium strains. | structurally identical teichoic acids were detected in cell walls of two soil isolates assigned to brevibacterium linens based on phylogenetic data. both cell walls contain unsubstituted 1,3-poly(glycerol phosphate) and poly(glycosylglycerol phosphate). repeating units of the latter--alpha-d-glcpnac-(1-->4)-beta-d-galp-(1-->1)-gro--are bound by phosphodiester bonds including oh-3 of galactose and oh-3 of glycerol. some of the n-acetylglucosamine residues have 4,6-pyruvic acid acetal, amounts of ... | 2004 | 15236605 |
| microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes. | in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ... | 2009 | 19477544 |
| the color of brevibacterium linens depends on the yeast used for cheese deacidification. | the color of smear cheeses (muenster) is traditionally thought to be due to the bacterial flora, e.g., brevibacterium linens. this study was carried out to evaluate indirect effects of yeast on the color of b. linens. a 60% cheese medium was desacidified with debaryomyces hansenii or kluyveromyces marxianus until ph 5.8 was reached. after inactivation of the yeast and addition of agar-nacl, b. linens was inoculated on the medium surface and incubated at 12 degrees c from d 2 to 28. for each bact ... | 2004 | 15291003 |
| controlled production of camembert-type cheeses. part i: microbiological and physicochemical evolutions. | a holistic approach of a mould cheese ripening is presented. the objective was to establish relationships between the different microbiological and biochemical changes during cheese ripening. model cheeses were prepared from pasteurized milk inoculated with kluyveromyces lactis, geotrichum candidum, penicillium camemberti and brevibacterium linens under aseptic conditions. two cheese-making trials with efficient control of environmental parameters were carried out and showed similar ripening cha ... | 2004 | 15354582 |
| response of sawmill workers to work-related airborne allergens. | the aim of this work was to determine the reactivity of sawmill workers to biological allergens associated with wood dust. allergological examinations by skin and precipitin tests were performed in 43 workers employed in a sawmill processing coniferous wood (pine), in 90 workers employed in two sawmills processing deciduous wood (oak), and in 32 healthy urban dwellers not exposed to organic dusts (referents). the skin test was performed by the intradermal method with the saline extracts of wood ... | 2001 | 11426929 |
| influence of adjunct cultures on ripening of reduced fat edam cheeses. | the influence of four adjunct cultures [brevibacterium linens (bl2), lactococcus lactis ssp. diacetylactis, lactobacillus helveticus (lh212), and lactobacillus reuteri (atcc 23272)] on chemical and sensory characteristics of reduced fat edam cheese was studied. the aminopeptidase activity of lactococcus lactis ssp. diacetylactis was higher than that of lactobacillus helveticus, lactobacillus reuteri, and brevibacterium linens, respectively. mean percent fat and moisture contents of reduced fat c ... | 2001 | 11699441 |
| anionic polymers of the cell wall of brevibacterium linens vkm ac-2159. | unsubstituted 1,3-poly(glycerol phosphate) and two sugar-1-phosphate polymers were identified in the cell wall of brevibacterium linens vkm ac-2159 by nmr spectroscopy and chemical methods. a monomer of one of the sugar-1-phosphate polymers has the branched repeating unit of the following structure: -4)-[beta-d-glcpnac-(1-->3)]-alpha-d-glcp-(1-p-. the repeating unit of another sugar-1-phosphate polymer has a linear structure consisting of alternating beta- and alpha-n-acetylglucosamine residues: ... | 2005 | 16266278 |
| heterologous carotenoid-biosynthetic enzymes: functional complementation and effects on carotenoid profiles in escherichia coli. | a limited number of carotenoid pathway genes from microbial sources have been studied for analyzing the pathway complementation in the heterologous host escherichia coli. in order to systematically investigate the functionality of carotenoid pathway enzymes in e. coli, the pathway genes of carotenogenic microorganisms (brevibacterium linens, corynebacterium glutamicum, rhodobacter sphaeroides, rhodobacter capsulatus, rhodopirellula baltica, and pantoea ananatis) were modified to form synthetic e ... | 2013 | 23144136 |