| long-term effect of bifidobacteria and neosugar on precursor lesions of colonic cancer in cf1 mice. | this investigation was undertaken to study the role of bifidobacteria and bifidogenic factor neosugar in the process of 1,2-dimethylhydrazine-induced colonic carcinogenesis in cf1 mice. intestinal colonization and selective proliferation of bifidobacteria were achieved by oral administration of indigenous bifidobacteria and the incorporation of 5% neosugar in the diet of animals. the bifidobacteria were isolated from the feces of cf1 mice and were identified to be bifidobacterium pseudolongum bi ... | 1991 | 1775387 |
| influence of whey and probiotic-supplemented withdrawal feed on the retention of salmonella intubated into market age broilers. | broilers (6 wk of age) were crop-intubated with a nalidixic acid-resistant strain of salmonella typhimurium and given four withdrawal feeds: 1) control feed (corn and soybean meal base, 18% cp and 3,200 kcal me/kg); 2) control plus 5% whey (61% lactose); 3) control plus probiotic (6.8 x 10(6) cfu of bifidobacterium pseudolongum, bifidobacterium thermophilium, and lactobacillus acidophilus); and 4) control plus 5% whey plus probiotic. in experiment 1, male broilers received 9.8 x 10(7) cfu of s. ... | 1990 | 2124688 |
| electron microscopic, biochemical and physiological studies of bifidobacterium pseudolongum ss-24 and bifidobacterium thermophilum ss-19. | comparative studies of physiology, biochemical characteristics, and morphology by electron microscopy were conducted on bifidobacterium pseudolongum ss-24 isolated from dogs and bifidobacterium thermophilum ss-19 isolated from swine. both b. pseudolongum and b. thermophilum grow unusually rapidly in the rumen fluid medium of scott and dehority, and reached a maximum of optical density after only 6 to 7 h of incubation. b. pseudolongum and b. thermophilum showed similar patterns of results for 21 ... | 1989 | 2803454 |
| [comparative studies on bifidobacteria isolated from the alimentary tract of man and animals (including descriptions of bifidobacterium thermophilum nov. spec. and bifidobacterium pseudolongum nov. spec)]. | | 1969 | 5361078 |
| effect of administration of bifidobacteria and lactic acid bacteria to newborn calves and piglets. | the effect of oral administration of bifidobacteria and lactic acid bacteria on newborn livestock was investigated. oral administration of bifidobacterium pseudolongum or lactobacillus acidophilus to calves improved bw gain and feed conversion over that of untreated controls. the frequency of diarrhea occurrence was decreased in the group fed probiotics. however, there was no significant difference between calves administered b. pseudolongum and those administered l. acidophilus. under condition ... | 1995 | 8675766 |
| digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples. | the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ... | 1997 | 9241587 |
| ecological and physiological studies on large intestinal bacteria in relation to production of hydrolytic and reductive enzymes involved in formation of genotoxic metabolites. | several hydrolytic and reductive bacterial enzymes (beta-glucuronidase, gn; beta-glucosidase, gs; arylsulphatase, as; azoreductase, ar; nitroreductase, nr) involved in production of mutagenic or genotoxic metabolites were measured in human colonic contents. cell-associated as and extracellular gs were approximately twice as high in the distal colon compared with the proximal bowel, while ar changed little throughout the gut. measurements of these enzymes in faeces from seven healthy donors confi ... | 1998 | 9879941 |
| evaluation of the hygienic quality of raw milk based on the presence of bifidobacteria: the cow as a source of faecal contamination. | fifty-eight samples of raw milk from three different farms were examined for the presence of bifidobacteria. isolates were identified and compared with bifidobacteria isolated from dung of the cows that provided the milk. of the raw milk samples, 88% harboured bifidobacterium pseudolongum subsp. globosum, as did 95% of the dung samples. | 2000 | 10777066 |
| molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal dna restriction analysis (ardra). | one hundred and six isolates of the genus bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal dna restriction analysis. two restriction endonucleases (sau3ai and bamhi) were chosen for aligning the 16s rrna sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. a rapid and accurate identification scheme was obtained ... | 2001 | 11451515 |
| adhesion of bifidobacteria to granular starch and its implications in probiotic technologies. | adhesion of 19 bifidobacterium strains to native maize, potato, oat, and barley starch granules was examined to investigate links between adhesion and substrate utilization and to determine if adhesion to starch could be exploited in probiotic food technologies. starch adhesion was not characteristic of all the bifidobacteria tested. adherent bacteria bound similarly to the different types of starch, and the binding capacity of the starch (number of bacteria per gram) correlated to the surface a ... | 2001 | 11472921 |
| fermentation of pectin and glucose, and activity of pectin-degrading enzymes in the rabbit caecal bacterium bifidobacterium pseudolongum. | in a rabbit caecal bacterium bifidobacterium pseudolongum, metabolites of pectin and glucose, and activities of enzymes involved in the degradation of pectin were assayed. simultaneously, activities of these enzymes were assayed in a rumen pectinolytic strain of streptococcus bovis. | 2002 | 12100587 |
| genomic diversity and relatedness of bifidobacteria isolated from a porcine cecum. | this study initially involved the isolation of a number of bifidobacteria from either the lumen or the epithelium of a porcine cecum. a total of 160 isolates were selected at random on mrs plates containing cysteine hydrochloride (0.5 g/liter) and mupirocin (50 mg/liter). all were identified as bifidobacteria based on fructose-6-phosphate phosphoketolase activity. following genomic digestion with the restriction enzyme xbai and pulsed-field gel electrophoresis (pfge), the isolates produced 15 di ... | 2003 | 12670982 |
| bifidobacterium species isolated from animal feces and from beef and pork meat. | bifidobacteria were isolated from 122 of 145 samples of animal feces (from cattle, swine, sheep, goats, horses, rabbits, chickens, geese, and pigeons) from farms in france and austria and from 92 of 955 production and processing chain samples of beef and pork (obtained at slaughter, cutting, and retail). bacterial strains were identified to species by phenotypic numerical classification based on api 50ch and id 32a tests and dna-dna hybridization. bifidobacterium pseudolongum was present in 81% ... | 2006 | 16629032 |
| reagentless identification of human bifidobacteria by intrinsic fluorescence. | a new identification method for bifidobacteria species from the human gastrointestinal tract was developed based on the measurement and statistical analysis of the intrinsic fluorescence of aromatic amino acids (aaa) and nucleic acids (na), following their excitation at 250 nm. the model was constructed by recording the fluorescence spectra of 53 bifidobacterium strains of 10 different species, including the corresponding type strains, and validated by analyzing the spectra data from nine furthe ... | 2007 | 17258337 |
| antibiotic susceptibility of bifidobacterium thermophilum and bifidobacterium pseudolongum isolates from animal sources. | the widespread use of antimicrobial substances has led to resistant populations of microorganisms in several ecosystems. in animal husbandry, the application of antibiotics has contributed to resistance development in pathogenic and commensal bacteria. these strains or their resistance genes can be spread along several ecological routes, including the food chain. antibiotic resistance is important in terms of the safety of industrial strains, such as probiotics for food and feed. bifidobacterium ... | 2007 | 17265870 |
| characterization of plasmid pasv479 from bifidobacterium pseudolongum subsp. globosum and its use for expression vector construction. | bifidobacterium pseudolongum subsp. globosum dpc479 is an intestinally-derived strain which contains a plasmid, pasv479, 4.8 kb in size. this plasmid has a g + c content of 59% and contains six open reading frames (orfs), four of which are cryptic. the other two orfs have 47% and 54% identity, respectively, to the replication and ftsk-like proteins found in a bifidobacterium breve ncfb 2258 plasmid, indicating that these plasmids, though isolated from differing bifidobacterium species, are relat ... | 2007 | 17467795 |
| antibiotic susceptibility testing of bifidobacterium thermophilum and bifidobacterium pseudolongum strains: broth microdilution vs. agar disc diffusion assay. | there is urgent need for having available suitable methods and data regarding the susceptibility levels of antibiotic resistant and sensitive strains of bifidobacteria. based on a defined standard operation procedure, agar disc diffusion and broth microdilution were compared in order to evaluate the antimicrobial susceptibility profiles of 82 b. pseudolongum and 80 b. thermophilum strains mainly originating from the meat production chain. the methods that were assessed showed interpretable agree ... | 2007 | 17884216 |
| bifidobacteria as indicators of faecal contamination along a sheep meat production chain. | the potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. the levels of bifidobacteria were compared with those of escherichia coli. total viable counts were followed along the chain (244 samples). | 2008 | 17922830 |
| utilization of resistant starch of native tapioca, corn and waxy corn starches and their retrograded preparations by bifidobacterium. | anaerobic fermentation of native starches from tapioca, normal and waxy corn, and their laboratory modified preparations, by selected bifidobacterium strains (bifidobacterium pseudolongum ksi9, bifidobacterium breve kn14, and bifidobacterium animalis ks20a1) was carried out under in vitro conditions. the growth and acidifying properties of bifidobacteria and utilization of resistant starches were determined in relation to glucose in the control sample. the preparations obtained from normal and w ... | 2008 | 18097846 |
| consumption of fructo-oligosaccharide reduces 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice. | strategies to manipulate the intestinal microbiota have been considered to promote immune health. the aim of the present study was to examine whether fructo-oligosaccharide, a typical prebiotic, could suppress antigen-specific skin inflammation by favourably changing the population of intestinal microbiota. female balb/c mice were fed a synthetic diet with or without fructo-oligosaccharide supplementation for 3 weeks and were then epicutaneously immunised with 2,4-dinitrofluorobenzene. afterward ... | 2008 | 18197992 |
| differential effects of bifidobacterium pseudolongum strain patronus and metronidazole in the rat gut. | in the luminal contents of metronidazole-treated rats, there was a dominant bifidobacterium species. a strain has been isolated, its 16s rrna gene has been sequenced, and the strain has been named bifidobacterium pseudolongum strain patronus. in this study, using an experimental model of healthy rats, the effects of metronidazole treatment and b. pseudolongum strain patronus administration on the luminal and mucosa-associated microbiota and on gut oxidation processes were investigated. metronida ... | 2009 | 19028910 |
| exploring the diversity of the bifidobacterial population in the human intestinal tract. | although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. we performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rrna gene sequences (16s rrna gene and internally transcribed spacer [its] 16s-23s spacer sequences) of isolated colonies. a total of 900 isolate ... | 2009 | 19168652 |
| role of intestinal bifidobacterium pseudolongum in dietary fructo-oligosaccharide inhibition of 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice. | strategies to manipulate the gut microbiota have been explored for preventing allergy development. we previously showed that dietary supplementation with fructo-oligosaccharide (fos) reduced 2, 4-dinitrofluorobenzene (dnfb)-induced contact hypersensitivity (chs) in balb/c mice. because the chs response was negatively correlated with the number of faecal bifidobacteria, particularly bifidobacterium pseudolongum, the present study aimed to examine whether oral administration of b. pseudolongum aff ... | 2010 | 20003567 |
| differentiation of bifidobacterium species using partial rna polymerase {beta}-subunit (rpob) gene sequences. | partial rna polymerase β-subunit gene (rpob) sequences (315 bp) were determined and used to differentiate the type strains of 23 species of the genus bifidobacterium. the sequences were compared with those of the partial hsp60 (604 bp) and 16s rrna genes (1475 or 1495 bp). the rpob gene sequences showed nucleotide sequence similarities ranging from 84.1 % to 99.0 %, while the similarities of the hsp60 sequences ranged from 78.5 % to 99.7 % and the 16s rrna gene sequence similarities ranged from ... | 2010 | 20061504 |
| production of conjugated linoleic acid and conjugated linolenic acid isomers by bifidobacterium species. | conjugated linoleic acid (cla) and conjugated linolenic acid (clna) isomers have attracted great interest because of their potential health benefits. formation of cla and clna takes place in the rumen during biohydrogenation. several studies have indicated that certain types of intestinal bacteria, including bifidobacteria, are able to convert linoleic acid (la) to cla. the role of intestinal bacteria in the formation of clna isomers is largely unknown. in the present study, a screening of 36 di ... | 2010 | 20556602 |
| metronidazole effects on microbiota and mucus layer thickness in the rat gut. | both mucus and mucosa-associated bacteria form a specific environment in the gut; their disruption may play a crucial role in the development of intestinal bowel disease (ibd). metronidazole, an antibiotic used in the treatment of ibd, alters gut microbiota and reduces basal oxidative stress to proteins in colonic tissue of healthy rats. the aim of this study was to evaluate the impact of the altered microbiota due to the metronidazole on the thickness of the mucus layer. this study was performe ... | 2010 | 20579100 |
| diversity of lactobacillus and bifidobacterium in feces of herbivores, omnivores and carnivores. | the lactobacillus and bifidobacterium population in the feces of 26 animals (16 species) were studied by culture-dependent and culture-independent techniques. lactobacilli were detected from a few herbivores, all carnivores and some omnivores. lactobacillus johnsonii, lactobacillus reuteri, lactobacillus salivarius, lactobacillus vaginalis and lactobacillus ingluviei were the most dominant lactobacilli in carnivores. these species were, however, not predominant in herbivores and omnivores. lacto ... | 2010 | 21034840 |
| bifidobacterium pseudolongum are efficient indicators of animal fecal contamination in raw milk cheese industry. | abstract: | 2011 | 21816092 |
| fucosyllactose and l-fucose utilization of infant bifidobacterium longum and bifidobacterium kashiwanohense. | human milk oligosaccharides (hmos) are one of the major glycan source of the infant gut microbiota. the two species that predominate the infant bifidobacteria community, bifidobacterium longum subsp. infantis and bifidobacterium bifidum, possess an arsenal of enzymes including α-fucosidases, sialidases, and β-galactosidases to metabolise hmos. recently bifidobacteria were obtained from the stool of six month old kenyan infants including species such as bifidobacterium kashiwanohense, and bifidob ... | 2016 | 27782805 |
| effects of antibiotics on bacterial species composition and metabolic activities in chemostats containing defined populations of human gut microorganisms. | the composition and metabolic activities of the human colonic microbiota are modulated by a number of external factors, including diet and antibiotic therapy. changes in the structure and metabolism of the gut microbiota may have long-term consequences for host health. the large intestine harbors a complex microbial ecosystem comprising several hundreds of different bacterial species, which complicates investigations on intestinal physiology and ecology. to facilitate such studies, a highly simp ... | 2013 | 23403424 |
| biosynthesis and cellular content of folate in bifidobacteria across host species with different diets. | bifidobacteria, one of the most common bacteria of the intestinal tract, help establish balance in the gut microbiota and confer health benefits to the host. one beneficial property is folate biosynthesis, which is dependent on species and strains. it is unclear whether the diversity in folate biosynthesis is due to the adaptation of the bifidobacteria to the host diet or whether it is related to the phylogeny of the animal host. to date, folate production has been studied in the bifidobacteria ... | 2014 | 25312826 |
| exercise induction of gut microbiota modifications in obese, non-obese and hypertensive rats. | obesity is a multifactor disease associated with cardiovascular disorders such as hypertension. recently, gut microbiota was linked to obesity pathogenesisand shown to influence the host metabolism. moreover, several factors such as host-genotype and life-style have been shown to modulate gut microbiota composition. exercise is a well-known agent used for the treatment of numerous pathologies, such as obesity and hypertension; it has recently been demonstrated to shape gut microbiota consortia. ... | 2014 | 24952588 |
| comparison of yacon (smallanthus sonchifolius) tuber with commercialized fructo-oligosaccharides (fos) in terms of physiology, fermentation products and intestinal microbial communities in rats. | the yacon (smallanthus sonchifolius) tuber was examined with regard to its prebiotic effects compared with commercialized fructo-oligosaccharides (fos). a feed containing 10% yacon tuber, which is equivalent to 5% commercialized fos in terms of the amount of fructo-oligosaccharides (gf2, gf3 and gf4), was administrated to rats for 28 days. the yacon diet changed the intestinal microbial communities beginning in the first week, resulting in a twofold greater concentration of cecal short-chain fat ... | 2013 | 24936376 |
| in vitro evaluation of bifidobacterium strains of human origin for potential use in probiotic functional foods. | the present study investigated some in vitro properties for probiotic use of four strains of bifidobacteria isolated from faeces of healthy children (bifidobacterium longum 51a, bifidobacterium breve 1101a, bifidobacterium pseudolongum 1191a and bifidobacterium bifidum 1622a). in vitro tests were carried out to compare growth rate, aerotolerance, antagonistic activity against pathogens, antimicrobial susceptibility profile and cell wall hydrophobicity. mean doubling time of b. longum 51a was sho ... | 2013 | 23443950 |
| conjugated linoleic and linolenic acid production kinetics by bifidobacteria differ among strains. | there is great interest in conjugated linoleic acid (cla) and conjugated linolenic acid (clna) isomers because of their supposed health-promoting properties. therefore, the differences in production kinetics of cla and clna isomers from linoleic acid (la) and α-linolenic acid (α-lna), respectively, by bifidobacteria were investigated. laboratory fermentations, supplemented with la or α-lna in the fermentation medium, were performed with bifidobacterium bifidum lmg 10645, bifidobacterium breve lm ... | 2012 | 22405353 |
| the extracellular proteome of two bifidobacterium species reveals different adaptation strategies to low iron conditions. | bifidobacteria are among the first anaerobic bacteria colonizing the gut. bifidobacteria require iron for growth and their iron-sequestration mechanisms are important for their fitness and possibly inhibit enteropathogens. here we used combined genomic and proteomic analyses to characterize adaptations to low iron conditions of b. kashiwanohense pv20-2 and b. pseudolongum pv8-2, 2 strains isolated from the feces of iron-deficient african infants and selected for their high iron-sequestering abil ... | 2017 | 28061804 |
| high iron-sequestrating bifidobacteria inhibit enteropathogen growth and adhesion to intestinal epithelial cells in vitro. | the gut microbiota plays an important role in host health, in particular by its barrier effect and competition with exogenous pathogenic bacteria. in the present study, the competition of bifidobacterium pseudolongum pv8-2 (bp pv8-2) and bifidobacterium kashiwanohense pv20-2 (bk pv20-2), isolated from anemic infant gut microbiota and selected for their high iron sequestration properties, was investigated against salmonella typhimurium (s. typhi) and escherichia coli o157:h45 (ehec) by using co-c ... | 2016 | 27713730 |
| effects of feed additives on ileal mucosa-associated microbiota composition of broiler chickens. | the effects of dietary supplementation with 2 recently developed feed additives on the composition of the mucosa-associated microbiota of the ileum were studied in growing broiler chickens. a total of 48 male 1-d-old broiler chickens of the cobb 500 strain were distributed in 4 treatments with 2 replicates of 6 birds each. the 2 additives tested were a di-d-fructose dianhydride–enriched caramel (fc) and the garlic derivative propyl propane thiosulfonate (pts-o). dietary treatments were a control ... | 2015 | 26440010 |
| bifidobacterium pseudolongum strain pv8-2, isolated from a stool sample of an anemic kenyan infant. | the complete genome sequence of bifidobacterium pseudolongum pv8-2, isolated from feces of an anemic kenyan infant, was determined using single-molecule real-time (smrt) technology. the genome consists of a 2-mbp chromosome and a 4-kb plasmid. | 2015 | 25614573 |
| dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats. | consumption of prebiotics may modulate gut microbiota, subsequently affecting the bacterial composition, metabolite profile, and human health. previous studies indicate that also changes in intestinal integrity may occur. in order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (xos) on the gut microbiota and intestinal integrity in male wistar rats. as changes in intestinal integrity may be related to the expected bifidogenic effect of xos ... | 2014 | 25238818 |