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comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms.the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ...19921331019
[invasiveness of bifidobacterium dentium iv-135 strain into tooth tissues]. 19873484208
[actinomyces eriksonii georg, robertstad, brinkman and hicklin 1965 identical with bifidobacterium adolescentis reuter 1963 (author's transl)]. 19744151212
bifidobacterium (actinomyces) eriksonii infection. 19744429260
extracellular dextranase activity produced by human oral strains of the genus bifidobacterium.three strains of anaerobic, dextranase-producing, gram-positive, rod-shaped bacteria were isolated from human dental plaque associated with root carious lesions. the isolates produced a molar ratio of acetate to lactate from glucose fermentation ranging from 1.1 to 1.9. each strain also produced fructose-6-phosphate phosphoketolase. the isolates were identified as belonging to the genus bifidobacterium, but from their carbohydrate fermentation patterns they did not appear to be strains of bifido ...19836642650
degradation of complex carbohydrates by bifidobacterium spp.two hundred and ninety strains of 29 species of bifidobacteria from human and animal origin were surveyed for their ability to ferment complex carbohydrates. the substrates fermented by the largest number of species were d-galactosamine, d-glucosamine, amylose and amylopectin. many of the species isolated from animal habitats showed reduced fermentation activity. bifidobacterium dentium strains fermented gum guar and gum locust bean; porcine gastric mucin was fermented only by b. bifidum, b. inf ...19947703014
isolation of a human intestinal anaerobe, bifidobacterium sp. strain sen, capable of hydrolyzing sennosides to sennidins.a strictly anaerobic bacterium capable of metabolizing sennosides was isolated from human feces and identified as bifidobacterium sp., named strain sen. the bacterium hydrolyzed sennosides a and b to sennidins a and b via sennidin a and b 8-monoglucosides, respectively. among nine species of bifidobacterium having beta-glucosidase activity, only bifidobacterium dentium and b. adolescentis metabolized sennoside b to sennidin b, suggesting that the sennoside-metabolizing bacteria produce a novel t ...19948161172
peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report.peritonsillar abscess is a potentially life-threatening complication of acute tonsillitis. on occasion, peritonsillar abscess can extend to neck spaces and/or to the mediastinum. we describe a case of a patient with a peritonsillar abscess that extended to the neck, producing bilateral retropharyngeal abscesses and myonecrosis of the strap muscles. culture of a specimen of the necrotic muscle yielded prevotella intermedia, prevotella buccae, lactobacillus catenaforme, another lactobacillus speci ...19938324135
rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests.eighty-nine species of subgingival bacteria, represented by 121 reference strains and 892 patient isolates, including gram-negative, gram-positive, aerobic, facultatively anaerobic, microaerophilic, and anaerobic species, were characterized with a panel of fluorogenic, 4-methylumbelliferyl-linked substrate tests. identifications of all patient isolates were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins relative to reference strains. char ...19968789019
bifidobacterium inopinatum sp. nov. and bifidobacterium denticolens sp. nov., two new species isolated from human dental caries.in a previous investigation of bifidobacteria isolated from human dental caries (v. scardovi and f. crociani, int. j. syst. bacteriol. 24:6-20, 1974), 40 strains were assigned to the new species bifidobacterium dentium. in this study we examined 70 new strains of bifidobacteria isolated from dental caries. the morphological characteristics, biochemical reactions, fermentation patterns, end products from glucose metabolism, protein electrophoretic patterns, levels of dna hybridization, and dna g+ ...19968934909
molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal dna restriction analysis (ardra).one hundred and six isolates of the genus bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal dna restriction analysis. two restriction endonucleases (sau3ai and bamhi) were chosen for aligning the 16s rrna sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. a rapid and accurate identification scheme was obtained ...200111451515
use of bifidobacterium dentium as an indicator of the origin of fecal water pollution.a new, simple, and specific protocol to discriminate between human and animal fecal pollution is described. the procedure is based on the detection of certain bifidobacterium species in the samples. two 16s rrna gene-targeted probes are described. one of these probes (bde) has as its target a region of the 16s rrna gene of bifidobacterium dentium, a bifidobacterium species of exclusively human origin. the other probe (ban) is based on the sequence of a region of 16s rrna gene for several bifidob ...200312732533
quantitative real-time pcr assays to identify and quantify fecal bifidobacterium species in infants receiving a prebiotic infant formula.a healthy intestinal microbiota is considered to be important for priming of the infants' mucosal and systemic immunity. breast-fed infants typically have an intestinal microbiota dominated by different bifidobacterium species. it has been described that allergic infants have different levels of specific bifidobacterium species than healthy infants. for the accurate quantification of bifidobacterium adolescentis, bifidobacterium angulatum, bifidobacterium bifidum, bifidobacterium breve, bifidoba ...200515870317
occurrence of the family bifidobacteriaceae in human dental caries and plaque.despite the constant presence of members of bifidobacteriaceae in the human oral cavity, few studies of their occurrence in this habitat have been made. the presence and the distribution in human plaque and dental caries of bifidobacterium dentium, scardovia inopinata and parascardovia denticolens, all belonging to the family bifidobacteriaceae, were studied. the identification to species level was based on morphological and growth type features, fermentation tests, polyacrylamide gel electropho ...200616707878
integrated analysis of established and novel microbial and chemical methods for microbial source tracking.several microbes and chemicals have been considered as potential tracers to identify fecal sources in the environment. however, to date, no one approach has been shown to accurately identify the origins of fecal pollution in aquatic environments. in this multilaboratory study, different microbial and chemical indicators were analyzed in order to distinguish human fecal sources from nonhuman fecal sources using wastewaters and slurries from diverse geographical areas within europe. twenty-six par ...200616957211
population dynamics of bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry.the population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (fish) coupled with flow cytometry (fcm) analysis. although double-staining fish-fcm using both fluorescein isothiocyanate (fitc) and indodicarbocyanine (cy5) as labeling dyes for fecal samples has been reported, the analysis was interfered with by strong autofluorescence at the fitc fluorescence region because of the presence ...200617056700
quantitative assessment of faecal bifidobacterial populations by real-time pcr using lanthanide probes.to develop real-time quantitative pcr methods, based on the use of probes labelled with a stable fluorescent lanthanide chelate, for the quantification of different human faecal bifidobacterial populations.200717381755
safety assessment of dairy microorganisms: propionibacterium and bifidobacterium.the genera propionibacterium and bifidobacterium are clustered in the class actinobacteria and form the anaerobic branch of coryneform bacteria. the dairy propionibacteria comprising four species p. freudenreichii, p. acidipropionici, p. jensenii and p. thoenii are industrially important as starter cultures in hard-cheese ripening and recently also as protective bio-preservatives and probiotics. these four species are considered as safe whereas cutaneous propionibacterium species (also named "ac ...200817889391
reduction of vitamin k concentration by salivary bifidobacterium strains and their possible nutritional competition with porphyromonas gingivalis.to assess the possibility that bifidobacteria compete with porphyromonas gingivalis for their mutual growth factor vitamin k. this study also examined whether salivary bifidobacterium species decrease vitamin k concentration in the growth medium.200717953607
gnotobiotic mouse immune response induced by bifidobacterium sp. strains isolated from infants.bifidobacterium, which is a dominant genus in infants' fecal flora and can be used as a probiotic, has shown beneficial effects in various pathologies, including allergic diseases, but its role in immunity has so far been little known. numerous studies have shown the crucial role of the initial intestinal colonization in the development of the intestinal immune system, and bifidobacteria could play a major role in this process. for a better understanding of the effect of bifidobacterium on the i ...200818083875
bacteria of dental caries in primary and permanent teeth in children and young adults.although streptococcus mutans has been implicated as a major etiological agent of dental caries, our cross-sectional preliminary study indicated that 10% of subjects with rampant caries in permanent teeth do not have detectable levels of s. mutans. our aims were to use molecular methods to detect all bacterial species associated with caries in primary and permanent teeth and to determine the bacterial profiles associated with different disease states. plaque was collected from 39 healthy control ...200818216213
effect of inulin on the human gut microbiota: stimulation of bifidobacterium adolescentis and faecalibacterium prausnitzii.prebiotics are food ingredients that improve health by modulating the colonic microbiota. the bifidogenic effect of the prebiotic inulin is well established; however, it remains unclear which species of bifidobacterium are stimulated in vivo and whether bacterial groups other than lactic acid bacteria are affected by inulin consumption. changes in the faecal microbiota composition were examined by real-time pcr in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in co ...200918590586
isolation and identification of bifidobacteriaceae from human saliva.bifidobacteriaceae were isolated from saliva and infected dentine by using a mupirocin-based selective medium. of the saliva samples, 94% harbored bifids. the mean concentration (+/- the standard error) was 4.46 (+/-0.12) log(10)(cfu per ml + 1), and the predominant isolates were bifidobacterium dentium, b. longum, scardovia inopinata, parascardovia denticolens, and alloscardovia omnicolens.200818723652
association between bifidobacteriaceae and the clinical severity of root caries lesions.the isolation of members of the family bifidobacteriaceae (bifids) from oral samples has been sporadic and a recent cloning study has suggested that they are not detectable in root caries lesions.200919121067
exploring the diversity of the bifidobacterial population in the human intestinal tract.although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. we performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rrna gene sequences (16s rrna gene and internally transcribed spacer [its] 16s-23s spacer sequences) of isolated colonies. a total of 900 isolate ...200919168652
evaluation of the passage of lactobacillus gasseri k7 and bifidobacteria from the stomach to intestines using a single reactor model.probiotic bacteria are thought to play an important role in the digestive system and therefore have to survive the passage from stomach to intestines. recently, a novel approach to simulate the passage from stomach to intestines in a single bioreactor was developed. the advantage of this automated one reactor system was the ability to test the influence of acid, bile salts and pancreatin.lactobacillus gasseri k7 is a strain isolated from infant faeces with properties making the strain interestin ...200919426473
the persistence of bifidobacteria populations in a river measured by molecular and culture techniques.to determine relative to faecal coliforms (fc) and sulfite-reducing clostridia (src), the environmental persistence of natural populations of bifidobacterium spp. enumerated by culturing and quantitative polymerase chain reaction (q-pcr).200919486397
the bifidobacterium dentium bd1 genome sequence reflects its genetic adaptation to the human oral cavity.bifidobacteria, one of the relatively dominant components of the human intestinal microbiota, are considered one of the key groups of beneficial intestinal bacteria (probiotic bacteria). however, in addition to health-promoting taxa, the genus bifidobacterium also includes bifidobacterium dentium, an opportunistic cariogenic pathogen. the genetic basis for the ability of b. dentium to survive in the oral cavity and contribute to caries development is not understood. the genome of b. dentium bd1, ...200920041198
[distribution of bifidobacterium in oral cavities of children and the relations with caries].to explore a selected-media of bifidobacterium from oral cavity, to detect the distribution of bifidobacterium in different sites of children and primarily investigate the relationship between oral bifidobacterium and early childhood caries.200920077895
molecular indicators used in the development of predictive models for microbial source tracking.a number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. no single one of the indicators tested to date has been able to determine the source of fecal pollution in water. however, the combined use of different indicators has been demonstrated to be the best way of defining predictive models suitable for determining fecal pollution sources. molecular methods are promising tools that could complement standard microbiolo ...201020118380
non-oral bifidobacteria and the aciduric microbiota of the denture plaque biofilm.the microbiota of the denture plaque biofilm colonizing the fitting surface of dentures in edentulous subjects with healthy palates (n = 20) and in edentulous subjects with denture stomatitis (n = 20) was studied. the numbers of bacteria colonizing the dentures of healthy subjects was significantly less than the numbers colonizing the dentures of stomatitis subjects. the proportions and frequency of isolation of mutans streptococci, lactobacilli, bifidobacteria and yeasts were significantly (p < ...201020536746
resistance to acidic environments of caries-associated bacteria: bifidobacterium dentium and bifidobacterium longum.oral bifidobacteriaceae, bifidobacterium dentium and bifidobacterium longum, are known to be isolated together with mutans streptococci and lactobacilli from caries lesions, suggesting that these bifidobacteriaceae are caries associated and acid resistant. this study aimed to investigate effects of acidification on b. dentium and b. longum, and to compare them with those on streptococcus mutans, streptococcus sanguinis and lactobacillus paracasei. effects of acidification, growth ability in a co ...201020814202
bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces.hm2-1 and hm2-2t strains were isolated from the faeces of a healthy infant and were characterised by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16s rrna gene sequence analysis. they were gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. they did not show growth at 15 and 45°c in abcm broth, and their dna g+c content is in the range of 56-59 mol%. in enzyme activity tests, hm2-1 ...201021148680
genomics and ecological overview of the genus bifidobacterium.members of the genus bifidobacterium are high g+c gram positive bacteria belonging to the phylum actinobacteria, and represent common inhabitants of the gastro-intestinal tract (git) of mammals, birds and certain cold-blooded animals. the overall microbial population that resides in the git, referred to as the "gut microbiota", is an extremely complex community of microorganisms whose functions are believed to have a significant impact on human physiology. different ecological relationships betw ...201021276626
changes of fecal bifidobacterium species in adult patients with hepatitis b virus-induced chronic liver disease.the beneficial effects of bifidobacteria on health have been widely accepted. patients with chronic liver disease have varying degrees of intestinal microflora imbalance with a decrease of total bifidobacterial counts. since different properties have been attributed to different bifidobacterium species and there is no information available for the detailed changes in the genus bifidobacterium in patients with chronic liver disease heretofore, it is meaningful to investigate the structure of this ...201121814872
genetic analysis and morphological identification of pilus-like structures in members of the genus bifidobacterium.background: cell surface pili in gram positive bacteria have been reported to orchestrate the colonization of host tissues, evasion of immunity and the development of biofilms. so far, little if any information is available on the presence of pilus-like structures in human gut commensals like bifidobacteria. results and discussion: in this report, atomic force microscopy (afm) of various bifidobacterial strains belonging to bifidobacterium bifidum, bifidobacterium longum subsp. longum, bifidobac ...201121995649
lab-test(®) 4: dental caries and bacteriological analysis.dental caries is one of the most common infectious ultifactorial diseases worldwide, characterized by the progressive demineralization of the tooth, following the action of bacterial acid metabolism. the main factors predisposing the onset of the carious process are: 1) the presence of bacterial species able to lower the ph until critical values of 5.5, 2) the absence of adequate oral hygiene, 3) an inefficient immune response anti-caries, 4) the type of alimentary diet and 5) the structure of t ...201223814571
comparative in vitro investigation of the cariogenic potential of bifidobacteria.this study aimed to assess the in vitro cariogenic potential of some bifidobacterium species in comparison with caries-associated bacteria.201627475723
bifidobacteria inhibit the growth of porphyromonas gingivalis but not of streptococcus mutans in an in vitro biofilm model.there is growing interest in the use of probiotic bifidobacteria for enhancement of the therapy, and in the prevention, of oral microbial diseases. however, the results of clinical studies assessing the effects of bifidobacteria on the oral microbiota are controversial, and the mechanisms of actions of probiotics in the oral cavity remain largely unknown. in addition, very little is known about the role of commensal bifidobacteria in oral health. our aim was to study the integration of the probi ...201627061393
effect of metalloporphyrins on red autofluorescence from oral bacteria.the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ...201323659237
predicting fecal sources in waters with diverse pollution loads using general and molecular host-specific indicators and applying machine learning methods.in this study we use a machine learning software (ichnaea) to generate predictive models for water samples with different concentrations of fecal contamination (point source, moderate and low). we applied several mst methods (host-specific bacteroides phages, mitochondrial dna genetic markers, bifidobacterium adolescentis and bifidobacterium dentium markers, and bifidobacterial host-specific qpcr), and general indicators (escherichia coli, enterococci and somatic coliphages) to evaluate the sour ...201525585145
biosynthesis and cellular content of folate in bifidobacteria across host species with different diets.bifidobacteria, one of the most common bacteria of the intestinal tract, help establish balance in the gut microbiota and confer health benefits to the host. one beneficial property is folate biosynthesis, which is dependent on species and strains. it is unclear whether the diversity in folate biosynthesis is due to the adaptation of the bifidobacteria to the host diet or whether it is related to the phylogeny of the animal host. to date, folate production has been studied in the bifidobacteria ...201425312826
the genome sequence of bifidobacterium moukalabense dsm 27321 highlights the close phylogenetic relatedness with the bifidobacterium dentium taxon.bifidobacterium moukalabense dsm 27321 is the reference strain for a recently described new bifidobacterial species that has been isolated from a wild west lowland gorilla. here, we report the whole-genome sequence of dsm 27321, which supports very close phylogenetic relatedness with members of the bifidobacterium adolescentis phylogenetic group and, in particular, the bifidobacterium dentium taxon.201424558236
isolation of a bifidogenic peptide from the pepsin hydrolysate of bovine lactoferrin.lactoferrin is an iron-binding glycoprotein found in the milk of most mammals for which various biological functions have been reported, such as antimicrobial activity and bifidogenic activity. in this study, we compared the bifidogenic activity of bovine lactoferrin (blf) and pepsin hydrolysate of blf (blfh), isolated bifidogenic peptide from blfh, and investigated the bifidogenic spectra of blf, blfh, and its active peptide against 42 bifidobacterial strains comprising nine species. against bi ...201323315725
acidogenicity of dual-species biofilms of bifidobacteria and streptococcus mutans.the aim of this study was to evaluate the acidogenicity of dual-species biofilms of bifidobacteria and streptococcus mutans.201627660160
analysis of bacterial activity in sound and cariogenic biofilm: a pilot in vivo study.dental caries is a multifactorial disease with many associated microbial taxa, but only a few are notably contributing to acidogenicity. the ribosome number and the corresponding 16s ribosomal rna (rrna) concentration are considered a molecular indicator for general metabolic activity of bacteria, as they are elevated with increased anabolic and catabolic activities. we hypothesize that the activity of aciduric/acidogenic bacterial taxa, reflected by a rise in ribosomal counts, could resolve dif ...201627595541
gaba-producing bifidobacterium dentium modulates visceral sensitivity in the intestine.recurrent abdominal pain is a common and costly health-care problem attributed, in part, to visceral hypersensitivity. increasing evidence suggests that gut bacteria contribute to abdominal pain perception by modulating the microbiome-gut-brain axis. however, specific microbial signals remain poorly defined. γ-aminobutyric acid (gaba) is a principal inhibitory neurotransmitter and a key regulator of abdominal and central pain perception from peripheral afferent neurons. although gut bacteria are ...201727458085
stability of saliva microbiota during moderate consumption of red wine.this study has evaluated the effect of regular and moderate red wine consumption on the diversity and occurrence of different groups of bacteria that are representative in human saliva.201526433355
aciduric microbial taxa including scardovia wiggsiae and bifidobacterium spp. in caries and caries free subjects.actinobacteria came into focus of being potential caries-associated pathogens and could, together with the established streptococcus mutans and lactobacilli thus function as caries indicator species. here we analyzed the role and diagnostic predictive value of the acidogenic-aciduric species scardovia wiggsiae and bifidobacterium dentium together with s. mutans, lactobacilli and bifidobacteria in biofilm of non-cavitated (n = 20) and cavitated (n = 6) caries lesions versus controls (n = 30). for ...201525933689
complete genome sequence of bifidobacterium dentium strain jcm 1195t, isolated from human dental caries.bifidobacterium dentium strain jcm 1195(t) was isolated from human dental caries. here, we report the complete genome sequence of this organism.201525858847
metabolism of rutin and poncirin by human intestinal microbiota and cloning of their metabolizing α-l-rhamnosidase from bifidobacterium dentium.to understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens. the average α-lrhamnosidase activities on the p-nitrophenyl-α-l-rhamnopyranoside, rutin, and poncirin subtrates were 0.10 ± 0.07, 0.25 ± 0.08, and 0.15 ± 0.09 pmol/min/mg, respectively. to investigate the enzymatic properties, α-l-rhamnosidase-producing bacteria ...201525179902
occurrence of bifidobacteriaceae in human hypochlorhydria stomach.the human stomach, when healthy, is not a suitable host for microorganisms, but in pathological conditions such as gastritis, when gastric acid secretion is impaired, microbial overgrowth can be observed. apart from helicobacter pylori, the composition of microbiota, resident or exogenously introduced during neutral/high ph conditions, has not been investigated thoroughly. thus, it is possible that bifidobacteriaceae, important autochthonous and beneficial bacteria of human gastrointestinal micr ...201424427111
assessment of microbiome variation during the perioperative period in liver transplant patients: a retrospective analysis.understanding the composition of the microbial populations in the intestines of liver transplant patients is important to preventing postoperative infection. we investigated the relationship between the risk of postoperative infection and variation in the predominant fecal microbial composition during the perioperative period. we prospectively analyzed the predominant intestinal microbiome of five asymptomatic adult carriers of hepatitis b virus (as controls without any antibiotics) at four week ...201323504024
γ-aminobutyric acid production by culturable bacteria from the human intestine.to assess the ability of human intestinally derived strains of lactobacillus and bifidobacterium to produce γ-aminobutyric acid (gaba).201222612585
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