| development and validation of a nested-pcr-denaturing gradient gel electrophoresis method for taxonomic characterization of bifidobacterial communities. | the taxonomic characterization of a bacterial community is difficult to combine with the monitoring of its temporal changes. none of the currently available identification techniques are able to visualize a "complete" community, whereas techniques designed for analyzing bacterial ecosystems generally display limited or labor-intensive identification potential. this paper describes the optimization and validation of a nested-pcr-denaturing gradient gel electrophoresis (dgge) approach for the spec ... | 2003 | 14602589 |
| bombiscardovia coagulans gen. nov., sp. nov., a new member of the family bifidobacteriaceae isolated from the digestive tract of bumblebees. | one hundred and eighty-seven fructose-6-phosphate phosphoketolase positive strains were isolated from the digestive tract of three different bumblebee species. analyses of the partial 16s rrna gene sequences of the representative strains showed only 92.8% and 92.5% similarity to bifidobacterium coryneforme yit 4092(t) and bifidobacterium indicum jcm 1302(t), 92.2% similarity to alloscardovia omnicolens ccug 18650 and slightly reduced similarity of 91% to other members of the family bifidobacteri ... | 2010 | 20950979 |
| molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal dna restriction analysis (ardra). | one hundred and six isolates of the genus bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal dna restriction analysis. two restriction endonucleases (sau3ai and bamhi) were chosen for aligning the 16s rrna sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. a rapid and accurate identification scheme was obtained ... | 2001 | 11451515 |
| bile salt toxicity to some bifidobacteria strains: role of conjugated bile salt hydrolase and ph. | the purpose of this work was to study some aspects of bile salt toxicity towards bifidobacteria. a strain (bifidobacterium coryneforme atcc 25911) was selected for its lack of conjugated bile salt hydrolase activity (cbsh-), and was used with three deconjugating strains (cbsh+), for study of their growth and viability in the presence of two dihydroxylated conjugated bile salts (tauro- and glyco-deoxycholic acids). the presence of the glycoconjugate induced a more significant growth inhibition fo ... | 2000 | 11068673 |