in vitro inhibition of growth of neisseria gonorrhoeae by genital microorganisms.the ability of microorganisms present in titers of over 10(5)/ml in the vaginal or cervical secretions to inhibit growth of n. gonorrhoeae in vitro was tested. study of a strain of n. gonorrhoeae against 77 microorganisms demonstrated that most strans of s. epidermidis, s. aureus and the one "gaffkya anaerobia" interfered with the growth of the n. gonorrhoeae. a minority of strains of s. viridans, neisseria, candida and bifidobacterium demonstrated interference. no strains of enterococcus, dipth ...197717163
factors affecting the incidence and anti-salmonella activity of the anaerobic caecal flora of the young chick.thirty-two different types of anaerobic bacteria isolated from chickens have been tested for anti-salmonella activity in vitro. under the conditions of the test only bacteroides hypermegas and a bifidobacterium sp. were shown to inhibit the salmonellas and this was attributed to the production of volatile fatty acids (vfa's) coupled with a low ph. when these organisms were tested in newly hatched chicks no inhibition of s. typhimurium occurred. possible explanations for this observation are cons ...197934658
urease-producing species of intestinal anaerobes and their activities.urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ...197936839
interactions between bifidobacterium bifidum n4 and escherichia coli k-12 in their mixed cultures.the interactions between bifidobacterium bifidum n4 (b. bifidum) and escherichia coli k-12 (e. coli) were investigated in their mixed cultures. under conditions in which both bacteria grew well in their pure culture, b. bifidum inhibited the growth of e. coli even when the latter was inoculated at 10(4)-fold and preincubated for 41 hr. the inhibition in the mixed cultures appeared when the ph values were reduced below 4.6. when the lowering of ph was prevented by the addition of naoh, no inhibit ...197837291
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. ix. metabolism and release of cellular lipids in the presence of antibiotics.inhibiton of cell wall synthesis caused simultaneously an increase in cellular phospho-and glycolipids and a marked release of these compounds to the medium. the composition of the cellular and the released glyco-and phospholipids was almost the same. antibiotics, which inhibit cell wall synthesis, did not influence glycolipid composition, but increased the relative and absolute amounts of disphosphatidylglycerol and its lysoderivatives. incorporation and chase experiments demonstrated a conside ...197664257
immunological relationships among transaldolases in the genus bifidobacterium.antisera were prepared against electrophoretically homogeneous transaldolase (dihydroxyacetone transferase, e.c. of bifidobacterium thermophilum (b. ruminale) ru326 (atcc25866), b. cuniculi ra93 (atcc27916) and b. 'minimum' (homology group) f392 (atcc 27538). crude extracts of eighty six strains previously assigned to twenty one species of the genus bifidobacterium on the basis of deoxyribonouclelic acid (dna) homology (dna-dna hybridization), were compared by double diffusion tests on ...197995384
preliminary quantification of immunological relationships among the transaldolases of the genus bifidobacterium.the immunological relatedness among the transaldolases (dihydroxyacetone transferase, e.c. of twenty species of the genus bifidobacterum has been tested by the microcomplement fixation method, using b. thermophilum (b. ruminale) ru326 (= atcc 25866), b. cuniculi ra93 (= atcc 27916) and b. 'minimum' (dna homology group) f392 (= atcc 27916) as references. based on the serological relationships of the transaldolases, expressed either as indices of dissimilarity or as immunological distance ...197995386
lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes.the respiratory quinones of 73 strains of gram-positive bacteria including spore-forming rods, lactic-acid bacteria and actinomyctes were examined. menaquinones with seven isoprenoid units (mk-7) were the main quinone type found in representatives of the genus bacillus and in sporolactobacillus inulinus. however, a strain of b. thuringiensis produced mk-8 in addition to mk-7, and strains of b. lentus and b. pantothenticus appeared to produce mk-9 and mk-8, respectively, with no mk-7. in the clos ...1979119033
[the use of antibiotic-resistant bifidobacterium biphidum and lactobacillus acidophilus in the course of antibiotic therapy]. 1979119605
characterization of the glycolysis pathway and the plasma membrane of lactobacillus and bifidobacterium strains.the characteristics of the glycolytic pathway and the plasma membrane of lactobacillus and bifidobacterium were studied. the enzyme system of glycolysis (hexokinase, glucokinase and pyruvate kinase) which is the main source of energy in the anaerobic condition was localized in the cell soluble fraction (cytoplasma) of all species examined. neither electron transfer chain components nor oxidase activities were found in anaerobically cultured lactobacillus and bifidobacterium. adenosine triphospha ...1976138331
aerobic and anaerobic flora in bladder urine of patients with indwelling urethral catheters.twenty-five bladder urine specimens from 13 patients with long-standing indwelling catheters were obtained by percutaneous suprapubic needle aspiration to avoid contamination by extraneous flora and cultured aerobically and anaerobically. twenty different species of organism were isolated from twenty-four positive specimens. the isolates were predominantly gram-negative aerobic or facultatively anaerobic bacilli, with occasional gram-positive aerobic cocci and yeasts. one patient had clostridium ...1976178084
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. viii. composition and metabolism of phospholipids at different stages and conditions of growth.1. the phospholipid content and composition of bifidobacterium bifidum var. pennsylvanicus is markedly influenced by the growth phase, the ph and the presence of human milk in the culture medium. 2. the lipid-phosphorus content of the cells increases during the first period of active growth, but decreases later. the lipid-phosphorus content of the cells in the stationary phase is at constant ph 5.5 about 45 percent of that at constant ph 6.8 and final ph 5.2. this difference is caused by a gener ...1975237545
[scanning electron microscopic observation of the genus bifidobacterium (lactobacillus bifidus) (author's transl)]. 1977284821
effect of intestinal bacteria on incidence of liver tumors in gnotobiotic c3h/he male mice.the effect of intestinal microflora on liver tumorigenesis was studied in gnotobiotic c3h/he male mice monoassociated, diassociated, or polyassociated with the following strains of intestinal bacteria: escherichia coli, streptococcus faecalis, bifidobacterium adolescentis, bifidobacterium infantis, clostridium indolis, c. paraputrificum, c. perfringens, c. innocuum, c. nexile, c. ramosum, c. clostridiiforme, bacteroides multiacidus, bacteroides fragilis, veillonella alcalescens, v. parvula, and ...1979292809
[antagonistic interrelationships of bifidobacterium bifidum i proteus vulgaris in vitro in the digestive tract of gnotobiotic chicks].the antagonistic relations between bacterium bifidum, strain i/850 phi, and proteus vulgaris, strain f-30, were studied. these organisms, when introduced together in equal doses into the digestive tract of gnotobiotic chickens in a single administration, were shown to create certain ecological correlations in various organs with the prevalence of bifidobacteria which exerted no negative influence on proteus vulgaris. the additional daily administration of bifidobacteria for 3 days running in dos ...1979382712
the prevalence of anaerobic bacteria in suprapubic bladder aspirates obtained from pregnant women.the incidence of asymptomatic bacteriuria due to aerobic, facultative anaerobic and strictly anaerobic bacteria during pregnancy was determined in 185 patients admitted for complications of pregnancy, using suprapubic bladder aspiration. anaerobic bacterial counts of 103 per ml urine or higher were detected in 6.4 per cent of the specimens. the incidence of anaerobic bacteriuria seems comparable to that of bacteriuria due to aerobic and facultative anaerobic micro organisms. in our group of pati ...1979388942
[short-term oral chemoprophylaxis before intestine surgery. quantitative determination of bacteria and fungi in stool specimens (author's transl)].the methods of quantitative analysis of aerobe and anaerobe microbes and fungi stool specimens are described. the results of the studies in health people are compared to the results in patients undergoing surgical treatment of intestinal tract. a group of these patients received neomycin and bacitracin orally as short-time chemoprophylaxis to diminish possible woundinfection and/or sepsis. after oral medication germs as bifidobacterium, bacteroides and clostridium (not cl. perfringens) are reduc ...1979452770
[first steps in the bacterial colonization of the digestive tract of neonates (author's transl)].meconium samples obtained from 14 children aged 18 to 51 h were subjected to a quantitative differential analysis. the meconium of one of the children (47 h old) was sterile; in those of the 11 infants aged less than 47 h the dominant flora was composed of either enterobacteria, mainly escherichia coli sensitive to usual antibiotics, or of streptococci. the genus acinetobacter constituted the dominant flora in one case only. staphylococcus, corynebacterium, clostridium (c. perfringens), bacteroi ...1979475219
[alteration of the normal intestinal flora in human sucklings within the last 20 years].analyses of the feces-flora and the feces-ph of breastfed infants and of infants nourished with commercial formulae (ki-na, milasan) performed since 20 years were evaluated. in both regimens decreasing numbers of bifidobacteria could be remarked. the dominating position of this groups of bacteria was limited more and more. since 1971 the bifidobacterium biotype, characteristic of feces of breastfed infants could be proved no more. simultaneously germs of the e. coli-group and neonmycine-resisten ...1979481569
recovery of anaerobic bacteria from pediatric patients. a one-year experience.during 1975, 115 speciments from the soft tissues, body fluids, and purulent sites in 105 pediatric ward and nursery patients were submitted to the clinical laboratory for anaerobic study, and 75% of the cultures were positive. in most instance specimens were collected in anaerobic transport tubes containing peptone yeast extract and glucose. in the laboratory, samples were processed by the anaerobic holding jar method. nineteen different anaerobic organisms were isolated; the average number of ...1979495592
purification and properties of udp-glucose (udp-galactose) pyrophosphorylase from bifidobacterium enzyme having both udp-glucose (udp-glc) and udp-galactose (udp-gal) pyrophosphorylase activities was purified to homogeneity from bifidobacterium bifidum. the molecular weight of the enzyme was about 200,000 and it appeared to be composed of four identical subunits. the purified enzyme showed almost the same reactivity towards udp-glc and udp-gal, and showed about 10% of this activity towards udp-xylose at 8 mm. the enzyme required magnesium ions for maximum activity. the apparent equilibriu ...1979500588
indole-3-lactic acid as a tryptophan metabolite produced by bifidobacterium spp.fifty-one strains of the genus bifidobacterium have been found to accumulate indole-3-lactic acid in culture broth. the isolated metabolite was identified through mass and nuclear magnetic resonance spectroscopy. all the microorganisms tested, as resting cells, have been shown to be able to convert l-tryptophan into l-indole-3-lactic acid.1979533277
comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline.the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ...1979543353
fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon.a total of 154 strains from 22 species of bifidobacterium, peptostreptococcus, lactobacillus, ruminococcus, coprococcus, eubacterium, and fusobacterium, which are present in high concentrations in the human colon, were surveyed for their ability to ferment 21 different complex carbohydrates. plant polysaccharides, including amylose, amylopectin, pectin, polygalacturonate, xylan, laminarin, guar gum, locust bean gum, gum ghatti, gum arabic, and gum tragacanth, were fermented by some strains from ...1977563214
effects of growth conditions on the lipid composition of bifidobacterium bifidum subsp. pennsylvanicum.lipid-phosphorus and lipid-galactose content and phospholipid and fatty acid composition of bifidobacterium bifidum subsp. pennsylvanicum were examined under a wide variety of growth conditions. cells from 29-c cultures contained less lipid-phosphorus than did cells from 37-c cultures, but their lipid-galactose content and phospholipid composition did not differ. at both temperatures, the growth phase influenced the lipid composition similarly. phosphate, mg2+ and k+ concentrations in the medium ...1977596859
effects of growth conditions on the ion composition of bifidobacterium bifidum subsp. pennsylvanicum.the cation content of bifidobacterium bifidum subsp. pennsylvanicum was markedly influenced by the washing procedure of the cells, by the growth phase and the temperature, and by the composition of the culture medium. optimal retention of cations was achieved by washing with 0.25 m mgcl2 at 20c. the intracellular na+ concentration rose during growth in normal medium to a constant value in the stationary phase, the k+ concentration rose in the exponential phase, but fell in the stationary phase. ...1977596860
case report: fatal anaerobic pulmonary infection due to bifidobacterium eriksonii.this case illustrates progressive anaerobic pleuropulmonary infection caused by bifidobacterium eriksonii that eventually was fatal. aspiration pneumonia, lung abscess, empyema, and finally necrotizing pneumonia characterized the clinical course. the organism is difficult to recover, but there is reason to suspect that it may be of greater etiologic importance than heretofore recognized in debilitated patients with pleuropulmonary infections. this patient was a 52-year-old alcoholic with periodo ...1978628649
nonsporing, anaerobic, gram-positive rods in saliva and the gingival crevice of humans.quantitative and qualitative examination of anaerobically isolated flora of the gingival crevice and saliva was carried out. it was found that half the organisms were anaerobes and that there were twice as many gram-positive organisms as there were gram-negative ones. rods were predominant in the gingival crevice (60.5%) and cocci in saliva (69.1%). of the total organisms, nonsporing, gram-positive anaerobic rods accounted for 24% in the gingival crevice and 9.7% in saliva. these organisms were ...1978646354
influence of high luminal po2 on the anaerobic bacteria of the rat colon.the influence of high luminal po2 on colonic anaerobes was investigated by chronically perfusing the colon of unrestrained rats with o2 or n2. a luminal po2 of approximately 700 mm hg did not significantly influence the fecal concentration of total anaerobes recovered on rabbit blood agar or of bifidobacterium sp. and bacteroides sp. recovered on selective media. however, the o2 infusion reduced the rat's excretion of ch4 (a metabolic product of anaerobic bacteria) to about 5% of the control val ...1978680497
degradation of polysaccharides by intestinal bacterial enzymes.polysaccharides with structures resembling components of dietary fiber were fermented by a number of species of anaerobic bacteria from the human colon. some strains also fermented glycoprotein mucins. the strains that fermented the widest range of polysaccharide substrates were in the two genera bacteroides and bifidobacterium. polysaccharide degrading enzymes from several bacteroides species have been studied, and in most cases the enzyme activities were cell bound rather than extracellular. i ...1978707363
[fine structure of bifidobacterium bifidum].cultures of bifidobacterium bifidum 1 were grown in liquid and semisolid nutrient media for 14 hours to 6 days. their preparations were then studied by electron microscopy on ultra-thin sections after a routine chemical fixation or on replicas after rapid freezing-fracturing and etching. this procedure made it possible to obtain new data about the structural organization of cells in the course of growth of cultures which were characterized by desynchronization of growth and division processes. t ...1978713883
presence of a single enzyme catalyzing the pyrophosphorolysis of udp-glucose and udp-galactose in bifidobacterium bifidum.the enzyme preparation catalyzing the pyrophosphorolyses of udp-glucose and udpgalactose almost at the same rate was purified about 900-fold from bifidobacterium bifidum grown on glucose medium. the two activities were always associated with each other, and their activity ratio was always constant throughout the purification steps. the final preparations was revealed homogeneous by polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. there was no significant ...1978718964
antitumor effect of bifidobacterium infantis in mice. 1978729960
bacteremia due to bifidobacterium, eubacterium or lactobacillus; twenty-one cases and review of the literature.twenty-one cases of bacteremia due to bifidobacterium, eubacterium and lactobacillus are described. transient bacteremic episodes with these organisms may follow trauma to the mouth, intestine, or vagina. the majority of the patients were female and most had an underlying condition that may have predisposed to bacteremia. ten of the patients died despite antibiotic treatment.1978749356
effects of antibiotics on the cation composition of bifidobacterium bifidum subspecies pennsylvanicum.cell wall-inhibitory antibiotics caused a marked reduction of intracellular k+ and a small increase of na+, together resulting in a decline of intracellular cation content. chloramphenicol and tetracycline increased intracellular k+ but actinomycin decreased both na+ and k+ content. the release and uptake of 86rb+ were similarly affected by the antibiotics as the k+ content. the osmotic resistance of the protoplasts isolated from antibiotic-treated cells was apparently increased by inhibitors of ...1978755153
susceptibility of anaerobic bacteria to carbenicillin, cefoxitin, and related drugs.the agar dilution technique was used for determination of the bacteriostatic activity of carbenicillin, penicillin g, cefazolin, cephaloridine, cefoxitin, and cephalothin agaomst a variety of anaerobic bacteria. carbenicillin showed a high level of activity at a concentration of smaller than or equal to 100 mug/ml; only five of 123 strains of bacteroides fragilis, one strain of bifidobacterium eriksonii, and one strain of clostridium bifermentans were resistant to a concentration of larger than ...1975804023
quantitative microflora of the vagina.this study enumerated the predominant vaginal flora in 25 healthy patients: 18 prenatal (six with and 12 without gonorrhea) and seven nonpregnant without gonorrhea. all 25 patients had aerobes and 19 of the 25 had anerobes isolated in titers of greater than or equal to 10(5) colony-forming units (cfu per milliliter). the most frequently isolated microorganism was the lactobacillus (aerobic and anaerobic species) followed by c. vaginale, viridans streptococci, anaerobic gram-positive cocci, and b ...1977831488
urease assay and urease-producing species of anaerobes in the bovine rumen and human feces.a growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that peptostreptococcus productus is often the most numerous urease-forming species in human feces. also, some fecal strains of ruminococcus albus, clostridium innocuum, and clostridium beijerinckii produced urease. single strains of fusobacterium prausnitzii, coprococcus catus, and streptococcus mitis th ...1977879770
on the specificity of the uridine diphospho-n-acetylmuramyl-alanyl-d-glutamic acid: diamino acid ligase of bifidobacterium globosum.the peptidoglycan of bifidobacterium globosum contains ornithine and lysine alternately in the same position of the peptide subunit. the uridine diphospho-n-acetylmuramyl-alanyl-d-glutamic acid: diamino acid ligase of this organism was purified 700-fold. since the activities for the incorporation of ornithine and lysine into uridine diphospho-n-acetylmuramyl-tripeptide did not separate during purification and since the incorporation of ornithine is competitively inhibited by lysine and vice vers ...1977931511
metabolic relationships between phospho(galacto)lipids in bifidobacterium bifidum var. pennsylvanicus. 1976955100
biosynthesis of phosphogalactolipids and diphosphatidylglycerol in a membrane fraction of bifidobacterium bifidum var. pennsylvanicus. 1976974091
[studies on the composition of the fecal flora of healthy dogs with the special references of lactobacillus flora and bifidobacterium flora (author's transl)].with the new method the fecal flora of 13 clinically healthy dogs aged 3 to 42 months was analysed qualitatively and quantitatively. it was characterized by that bacteroidaceae constituted the most prodominant flora, catenabacteria, streptococci, peptostreptococci, lactobacilli and bifidobacteria were the next most numerous, enterobacteria consisted the accompanying flora. the numbers of clostridium perfringens were remarkably fluctuated and seem to be influenced by the composition of the food i ...1976983534
[composition of nucleic acids and concentration of methylated bases in the dna of bifidobacterium bifidum var. tissier].nucleotide composition of the sum total dna of b. bifidum, biotype iii, was determined by paper chromatography in combination with ultraviolet spectrophotometry. dna of b. bifidum was referred to the gc-type (gc -- 62.6 mol%). two additional nitrogen bases were present in the dna composition; 5-methylcytosine and 6-methylaminopurine -- 0.45 mol% and 0.20 mol%, respectively. nucleotide composition of the sum total rna was studied with the aid of high-voltage electrophoresis in combination with ul ...1976983588
presence of bifidobacteria in the rumen of calves fed different rations.a study was made on the numbers and species of bifidobacteria present in the rumen of calves fed high-roughage and high-concentrate diets. with the roughage ration the bifidobacteria were not detectable in a 10(-3) dilution, whereas with the concentrate ration their number was high, usually in the order of 10(8) to 10(9)/ml of rumen fluid. the species most represented, identified by means of deoxyribonucleic acid-deoxyribonucleic acid hybridization tests, included bifidobacterium ruminale, bifid ...1976984825
effects of antibiotics on metabolism of peptidoglycan, protein, and lipids in bifidobacterium bifidum subsp. pennsylvanicus.the formation of cell envelope components of bifidobacterium bifidum subsp. pennsylvanicus was studied by measuring the incorporation of [(3)h]glycine, (14)c-labeled fatty acids, and n-benzoyl-[(14)c]glucosamine into the membrane protein, membrane lipids, and cell wall peptidoglycan, respectively. inhibition of peptidoglycan synthesis by antibiotics (penicillin g, vancomycin, d-cycloserine, and bacitracin) and by the omission of glucosamine-containing growth factors caused a marked decrease in g ...19761008539
purification and properties of two fructose-6-phosphate phosphoketolases in bifidobacterium.fructose-6-phosphate phosphoketolase was purified from type strains of two species of the genus bifidobacterium: b. globosum and b. dentium. the first species has a preferred "animal" habitat, like feces of animals and rumen of cattle; the latter is harboured in "human" habitat, like feces and dental caries of man. two electrophoretic types of phosphoketolase (f6ppk) were previously distinguished and called "animal" and "human" type according to the habitat of the bifid organism. the purified pr ...19761085128
disk susceptibility testing of slow-growing anaerobic bacteria.the susceptibility of 55 strains of slow-growing anaerobes to eight clinically useful or potentially useful antibiotics was determined by agar dilution and disk diffusion tests. strains of the genera peptococcus, peptostreptococcus, megasphaera, veillonella, eubacterium, bifidobacterium, clostridium, and fusobacterium were included. all strains were susceptible to chloramphenicol, but varied in their susceptibility to penicillin, lincomycin, clindamycin, tetracyclines, and vancomycin. correlatio ...19751137353
effects of high risk and low risk diets for colon carcinogenesis on fecal microflora and steroids in man.we investigated the effects of a high meat mixed western diet and a nonmeat diet, representing the dietary pattern of high and low risk areas for colon cancer, respectively, on fecal microflora dn on bile acid and neutral sterol patterns in man. the total anaerobic microflora as well as the count of bacteroides, bifidobacterium, peptococcus, and anaerobic lactobacillus were significantly higher during the period of consumption of a high meat mixed western diet comparted with the nonmeat-diet con ...19751138032
scanning and transmission electron microscopy of three strains of bifidobacterium.scanning and transmission electron microscopy was applied for a morphological study of three strains of bifidobacterium grown on solid or liquid media. the pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. a possible sequence of the morphological events during transformation from one to another pleomorphic form is proposed for b. bifidum and b. longum. ultrastructural differences such as the formation of extensive mesosomal complexes in b. longum and ...19751182610
numerical taxonomy and laboratory identification of actinomyces and arachnia and some related bacteria.a numerical taxonomic study was made on 49 facultative anaerobic gram-positive filamentous and/or diphtheroidal organisms isolated from dental plaques, carious dentin and faeces, together with 63 reference strains belonging to the genera actinomyces, arachnia, bifidobacterium, actinobacterium, propionibacterium, eubacterium and lactobacillus. they were examined for 90 unit characters covering a wide range of tests and properties. the data were subjected to computer analysis in which the simple m ...19751202157
[antibacterial and bactericidal activity of tinidazole against anaerobic bacteria comparing with metronidazole (author's transl)].antibacterial activity of tinidazole (1-2-(ethylsulfonyl)-2-methyl-5-nitroimidazole) against anaerobic bacteria including peptococcus, peptostreptococcus, eubacterium, propionibacterium, bacteroides and fusobacterium was studied by agar dilution method comparing with metronidazole. in addition to this work, bactericidal effect of tinidazole and metronidazole against p. prevotii, b. fragilis ss. fragilis and f. varium was examined by quantitative culture method after incubation in gam broth conta ...19761271586
species-specific oligonucleotide probes for five bifidobacterium species detected in human intestinal microflora.portions of the 16s rrna from closely related species of the genus bifidobacterium that are found in the human intestinal microflora were sequenced in order to design species-specific oligonucleotide probes. five oligonucleotide probes ranging from 16 to 19 bases in length and complementary to 16s rrna sequences from bifidobacterium adolescentis, b. bifidum, b. breve, b. infantis, and b. longum were synthesized. with crude high-molecular-weight rna preparations as targets, these probes showed th ...19921282315
high-affinity oxygen uptake by bifidobacterium bifidum.oxygen uptake by washed cell suspensions of bifidobacterium bifidum dsm 20082 was studied by using spectrophotometric measurements of the degree of oxygenation of added myoglobin as a measure of the concentration of dissolved o2. the absorbance changes during consumption of o2 in a closed reaction vessel were analysed by computer to obtain estimates of the changes in dissolved o2 concentration with time. these were then used to calculate the rate of o2 uptake as a function of the dissolved o2 co ...19921285646
mechanism of allosteric transition of bacterial l-lactate dehydrogenase.the allosteric behaviour of l-lactate dehydrogenase (l-lactate:nad+oxidoreductase, ec, ldh) from bifidobacterium longum am101-2 was studied by means of the subunit hybridization technique as well as x-ray crystallography. homotropic allosteric response of the ldh activity was found against the concentration of its substrate, pyruvate. heterotropic allosteric activation of the enzyme was induced by the addition of fructose 1,6-bisphosphate (fbp). two mutant enzymes, which had either alte ...19921290930
effects of cultured milk products by lactobacillus and bifidobacterium species on the secretion of bile acids in hepatocytes and in rats.whey preparations prepared from cultured milk by 19 lactobacillus (2 species) and 20 bifidobacterium (5 species) strains were examined for the effects of secretion and synthesis of bile acids in primary cultured rat hepatocytes. the stimulating effect of whey preparation on bile acid secretion depended on the species as well as the strains used for milk fermentation. two strains belonging to l. casei sbt 2230 (lc2230) and b. longum sbt 2912 (bl2912) produced the whey which stimulates both the se ...19921291639
[immunomodulating properties of various microbes--representatives of normal intestinal microflora].the impact of oral administration of heat-killed bifidobacteria, lactobacteria, enterococci and bacteroides on mouse resistance to experimental salmonellosis and content of immunoglobulin-synthesizing cells in the proper plate of the small intestine was studied. it was shown that the administration of the killed bifidobacteria, lactobacteria and enterococci increased the animal resistance to experimental salmonellosis infection and induced an increase in the content of immunoglobulin-synthesizin ...19921304130
lithium chloride-sodium propionate agar for the enumeration of bifidobacteria in fermented dairy products.lithium chloride-sodium propionate agar has been developed for the enumeration of bifidobacteria in fermented dairy products. the medium contains lithium chloride and sodium propionate to inhibit the growth of other lactic acid bacteria. pure cultures of bifidobacteria, lactobacilli, and streptococci were tested for growth in this medium. with one exception, all bifidobacteria were able to grow in this medium and in a nonselective agar with a difference not exceeding .4 log units. however, none ...19921317893
comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms.the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ...19921331019
characterization and molecular cloning of bifidobacterium longum cryptic plasmid pmb1.the small cryptic plasmid pmb1 (1.9 kb), previously isolated from bifidobacterium longum, has been characterized by physical mapping. two cloning vectors, pmr3 and pdg7, carrying chloramphenicol and ampicillin resistances derived from pjh101, have been electroporated in escherichia coli.19901366860
effect of depolymerized alginates on the growth of bifidobacteria. 19921368312
phase variations in bifidobacterium animalis.strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. the rate of transition in a switching system from opaque to transparent colonies and vice versa was determined. differences in protein components and in penicillin-binding proteins (pbps) of the cells from different colony types are shown.19921369190
phylogenetic evidence for the transfer of eubacterium suis to the genus actinomyces as actinomyces suis comb. nov.the 16s rrna primary structures of eubacterium suis dsm 20639t (t = type strain) and bifidobacterium bifidum dsm 20456t were determined by sequencing in vitro amplified rdna. sequence comparisons indicated that b. bifidum is moderately related to representatives of the genera actinomyces and mobiluncus. the closest relative of e. suis is actinomyces pyogenes. e. suis and a. pyogenes are more closely related phylogenetically to one another than to the other actinomyces species that have been inve ...19921371060
isolation and identification of intestinal bacteria from japanese tree frog (hlya japonica) with the special reference to anaerobic bacteria.the bacteria in the large intestines of eight japanese tree frogs (hlya japonica) were enumerated by using an anaerobic culture system. the microorganisms at approximately 3.1 x 10(9) bacteria per g (wet weight) of intestinal contents were present in the intestine of all the frogs tested. no difference of the total bacteria in the frog intestine was observed between two different incubation-temperatures (room temperature and 37 degrees c). eleven genera and 16 species were isolated from the frog ...19921391180
impact of the advances in age on the gastrointestinal microflora of beagle dogs.the gastrointestinal microflora of male beagle dogs in two different age groups; i) less than month 12 of age, and ii) more than year 11 of age, was compared. no detectable difference occurred on the microflora of stomach, duodenum, jejunum, and ileum in both dogs. large bowel (cecum, colon and rectum) microflora in both dogs yielded the different microbial populations. in all regions of large bowel, the levels of bacteroides, eubacteria, peptostreptococci, bifidobacteria, lactobacilli, and stap ...19921391181
impact of bifidobacterium longum on human fecal microflora.the effects of bifidobacterium longum feedings for five weeks on the fecal microflora, water contents, ph values, ammonia concentration, and beta-glucuronidase activity were investigated in five healthy human volunteers. although numbers of major bacterial groups of the fecal microflora were not changed by the bifidobacteria feedings, a remarkably decreasing number of lecithinase-negative clostridia was observed. the percentage of lecithinase-negative clostridia and bacteroides to the total bact ...19921406371
[the intestinal microflora of persons subjected to a radiation lesion].the content of large intestine has been studied in persons exposed to radiation injury in consequence of the accident at the chernobyl atomic power plant. it is stated that bifidobacteria (10(7)-10(10) cells in 1 g of feces) prevailed (as in healthy people), bifidobacterium indicum being a dominating species. amount of lactic-acid bacteria in 1 g of defecations of examined patients was within the range of 10(6)-10(9) cells and in certain persons it reached 10(10) cells (primarily fecal enterococ ...19921406381
anaerobic microflora associated with the pars oesophagea of the healthy pigs aged one to 35 days significant populations of microorganisms (5.5 to 6.9 log10 viable count cm-2 of the tissue) were found, by strict anaerobic techniques, to adhere to the pars oesophagea. the genera of anaerobes which were isolated included actinomyces, bifidobacterium, clostridium, lactobacillus, peptostreptococcus, streptococcus and veillonella and facultative anaerobes included escherichia, klebsiella, staphylococcus, streptococcus and yeasts. the microbial population adher ...19921410807
defined cultures and prospects.resistance of young chicks to salmonella colonization can be increased by treating them with suspensions or anaerobic cultures of gut content from healthy, adult birds. unfortunately, such treatments have an unknown bacterial composition and are therefore not acceptable to regulatory agencies in some countries. efforts are continuing to identify components in the gut microflora that are involved in the protective process. the ultimate aim is to identify bacteria that may be used, either alone or ...19921419530
transient colonization of the gut of newborn infants by orally administered bifidobacteria and lactobacilli.we investigated if orally administered bifidobacteria and/or lactobacilli could be cultured from faeces of infants after antibiotic treatment, when these bacterial species are usually absent. lyophilized bifidobacterium longum, strain bb-536, b. breve, strain bb-576, or lactobacillus acidophilus, strain lac-343, were used. doses of 3 x 10(9) cells of one strain, or a mixture of all three strains 3 x 10(9) cells each were fed three times daily at mealtimes to 11 infants aged 0-8 weeks. treatment ...19921421883
[phosphate concentration. does reduction in infant formula feeding modify the micro-ecology of the intestine?].the potential influence of phosphates in formulas on intestinal microflora was studied in 25 infants, aged 8 days to 12 weeks. the babies were either fed an infant formula with the usual phosphate concentration (n = 10) or an infant formula with reduced phosphate and protein concentrations (n = 7). the microbiological findings were compared with those obtained from breastfed infants (n = 8). low-phosphate concentrations did not correlate with a predominance of bifidobacteria or suppression of pu ...19921435826
cloning and sequence analysis of the gene encoding l-lactate dehydrogenase from lactococcus lactis: evolutionary relationships between 21 different ldh enzymes.lactate dehydrogenase (ldh; ec1.1.1.27) is a key enzyme in the fermentation of milk by lactic acid bacteria used in the dairy industry. an 800-bp dna fragment containing part of the gene (ldh) encoding ldh was amplified from lactococcus lactis in a polymerase chain reaction using primers designed from the partial amino acid sequence of a lactococcal ldh. this fragment was radioactively labelled and used to probe a phage lambda library of lc. lactis genomic dna. fragments containing ldh were subc ...19921452029
[the effect of phospholipids on the adhesive properties of bacteria].the present work shows that choline-containing phospholipids (lysophosphatidylcholine and lyso-1-alkyl-sn-glycerophosphocholine) inhibit the adhesion of some strains: bacterium bifidum 1, b. adolescentis mc-42, b. longum b. 379m, staphylococcus aureus p 209 and klebsiella pneumoniae 52. phosphatidylcholine produces no effect on the adhesiveness of these strains, while platelet activation factor stimulates adhesiveness only in strain s. aureus 209. the stimulating or inhibiting action of phosphol ...19921455967
faecal steroids and colorectal cancer: the effect of lactulose on faecal bacterial metabolism in a continuous culture model of the large intestine.the effect of lactulose on intestinal bacterial metabolism in two identical single-stage chemostats has been studied. the study was designed as a single stage crossover. both cultures were inoculated and treated in an identical manner except that whilst one fermenter was subject to ph control the other was not and vice versa. complex bacterial populations were maintained for 23 days and many of the metabolic functions carried out by the micro-organisms in vivo were demonstrated in vitro. the pre ...19921463973
relationship between oxygen sensitivity and oxygen metabolism of bifidobacterium species.bifidobacteria, which are obligate anaerobes, were studied to determine the relationship between their sensitivity to oxygen and oxygen metabolism. among the four species tested, bifidobacterium infantis, bifidobacterium breve, and bifidobacterium longum differed from bifidobacterium adolescentis in sensitivity to oxygen. the former three species showed marked growth under conditions of partial aeration, whereas the growth of b. adolescentis was suppressed by low concentrations of oxygen. bifido ...19921474198
[clostridium difficile and diarrhea in infants in the first half-year of life].in order to study a possible etiological relationship between clostridium and diarrhea in children of the first half year of life and to characterize the colonization of the intestine with these bacteria, bacteriological investigations of feces were carried out in neonates and babies aged 1, 4 and 14 days and 1, 3 and 6 months. the development of the children and their health status were monitored under home conditions. it has been established that the colonization of the neonates' intestine wit ...19921475133
[effect of meropenem on fecal flora in children].meropenem (mepm, sm-7338), a novel parenteral carbapenem antibiotic, was examined for its effect on intestinal flora in children. seven children with infectious diseases (3 male and 4 female children of age's ranging from 4 months to 8 years and 9 months weighing from 7.3 to 23.0 kg) were treated with mepm at doses ranging 10.3 to 40.5 mg/kg 3 or 4 times a day for 6 to 12 days. before, during and after the treatment, identities and numbers of various bacteria contained in 1 g of feces were deter ...19921479688
antibacterial spectrum of lactoferricin b, a potent bactericidal peptide derived from the n-terminal region of bovine lactoferrin.a physiologically diverse range of gram-positive and gram-negative bacteria was found to be susceptible to inhibition and inactivation by lactoferricin b, a peptide produced by gastric pepsin digestion of bovine lactoferrin. the list of susceptible organisms includes escherichia coli, salmonella enteritidis, klebsiella pneumoniae, proteus vulgaris, yersinia enterocolitica, pseudomonas aeruginosa, campylobacter jejuni, staphylococcus aureus, streptococcus mutans, corynebacterium diphtheriae, list ...19921490908
[the influence of cefdinir on the intestinal bacterial flora].the influence of cefdinir (cfdn), a new oral cephalosporin, on the intestinal bacterial flora was studied in tetra-contaminated mice and in pediatric patients. cfdn in fine granules was administered at a dose of 10 mg/kg once a day for 5 consecutive days to mice contaminated with 4 different species of organism: escherichia coli, enterococcus faecalis, bacteroides fragilis and bifidobacterium breve. no remarkable changes were observed in the fecal viable cell counts except that decreases in e. c ...19921495195
host-microflora correlations in infant nutrition.the intestinal microflora represents an enormous cell mass and has a high metabolic capacity. the symbiotic efficacy of these microbes in humans is still a matter of discussion. of particular interest, from a biogenetic point of view, are potential symbiotic relations between the bifidobacterial microflora and the breast-fed infant. our group has conducted studies related to this topic; they were aimed at determining the dimension of microbial assimilation by the host. our studies with 15n-label ...19921496120
[the biological properties of bifidobacterium].the possibility of the formation of exoenzymes, such as dnaase, rnaase and hemolysin, by bifidobacteria was studied in comparison with their acid-forming and adhesive activity. bifidobacterium reference strains, originally isolated from healthy adults and children, were studied. the study involved altogether 73 strains of bifidobacteria, including 24 b. bifidum strains, 13 b. adolescentis strains, 7 b. infantis strains, 10 b. breve strains and 19 b. longum strains. the bifidobacteria under study ...19921496883
[the effect of different bacterial preparations in combination with total gnotobiological isolation on the survival of animals with experimental acute radiation sickness]. 19921496888
survival of lactobacillus acidophilus and bifidobacterium bifidum in ice cream for use as a probiotic food.probiotic ice cream was made by fermenting a standard ice cream mix with lactobacillus acidophilus and bifidobacterium bifidum cultures and then freezing the mix in a batch freezer. survival of the l. acidophilus and b. bifidum, as well as beta-galactosidase activity, was monitored during 17 wk of frozen storage at -29 degrees c. after freezing of the fermented mix, bacterial counts were 1.5 x 10(8) cfu/ml for l. acidophilus and 2.5 x 10(8) cfu/ml for b. bifidum. seventeen weeks after freezing, ...19921500547
[clinical and pharmacokinetic evaluation of meropenem and its effect on fecal flora in children].forty-five children were treated with meropenem (mepm) and the clinical efficacy and side effects were evaluated. the ages of the patients ranged from 1 month to 9 years and their body weights from 5.2 to 25 kg. doses given were 17.2-45.5 mg/kg every 6 to 8 hours for 2 to 24.5 days. those patients who responded to the mepm treatment included 15 children with pneumonia, 7 with pharyngitis, 3 with cervical lymphadenitis, 3 with cellulitis, 10 with urinary tract infections and 4 with other infectio ...19921507402
supplementation of an adapted formula with bovine lactoferrin: 1. effect on the infant faecal flora.the development of the infant faecal flora was studied over the first three months of life in infants receiving breast milk, a modern adapted formula and adaptations of this formula. breast-fed infants developed a flora rich in bifidobacterium sp. facultative anaerobes were ubiquitous, but in relatively small numbers within the diet group. other obligate anaerobes, such as clostridium sp. and bacteriodes sp. were rarely isolated. standard formula produced a flora rich in bifidobacteria, but the ...19921515754
[the effect of lactobacillus acidophilus and bifidobacterium bifidum on the intestinal ecosystem of the elderly patient].the authors have tested the therapeutic efficacy of a multibacterial combination consisting of lactobacillus acidophilus (10(9)) and bifidobacterium bifidum (10(9)) in elderly patients with bowel disorders. bacteriological and histopathologic investigation showed this combination to yield excellent biologic results with restoration of duodenal bacterial flora and subsidence of clinical symptoms. the function of the muciparous glands was restored and the duodenal mucosa was normalized.19921526095
in vitro hydrogen production by enteric bacteria cultured from children with small bowel bacterial overgrowth.lactulose breath hydrogen test and enterotest string test were carried out simultaneously on 19 children 3-5 years old. bacteria isolated from the jejunal fluid in upper small intestines of these children were incubated with lactulose at neutral ph. anaerobes were present in all but one child, and in 15 children they were present in numbers greater than 5 log10 organisms per ml. most of these bacteria did not produce hydrogen in vitro. hydrogen production (greater than 100 ppm) was observed with ...19921534365
are intestinal bacteria involved in the etiology of rheumatoid arthritis? review article.observations in bowel-related joint diseases give support to this hypothesis. in crohn's disease and ulcerative colitis, the bowel wall inflammation is complicated in about 20% of the patients by joint inflammation. bowel infection by salmonella, shigella and yersinia can provoke joint inflammation and supports an etiological link between bowel bacteria and arthritis. the arthropathic properties of the most abundant group of intestinal bacteria, i.e. the obligate anaerobic bacteria, were studied ...19921536716
[survival of lactobacillus acidophilus and bifidobacterium sp. in the small intestine following ingestion in fermented milk. a rational basis for the use of probiotics in man].oro-ileal intubation was performed in 6 healthy volunteers who ingested, either 100 g of a fermented milk containing 10(8)/g lactobacillus acidophilus and 10(7)/g bifidobacterium sp or sterilized fermented milk along with a meal in random order. lactobacillus acidophilus and bifidobacterium were counted in the ileal fluid which was aspirated continuously for 8 h, and flow rates were calculated using the constant slow infusion of peg 4000. after ingestion of fermented milk but not after control, ...19921537478
fecal recovery in humans of viable bifidobacterium sp ingested in fermented milk.bifidobacterium sp is a natural component of the dominant colonic microflora that was recently introduced into several fermented dairy products. the aim of the present study was to study the fate of this microorganism in the human gut. on the basis of antibiotic resistance characters, a variant of bifidobacterium sp that could be distinguished from indigenous bifidobacteria in the fecal flora was selected, and its survival and colonization in the colon was examined. this strain was used to ferme ...19921537524
regulation of the bacterial intestinal implantation in infant born by caesarean section.studies were undertaken to determine the regulation of the bacterial intestinal implantation in 19 newborns delivered by caesarean section. correlation was made with the infant feeding mode. the effect of human milk seemed to be the result of b. bifidum proliferation, in contrast to artificial alimentation that seemed to favour c. perfringens implantation. the question was raised by us as to whether this opposition was only related to alimentation. in fact, b. bifidum itself also had an effect a ...19921547622
identification of bifidobacteria from fermented milk products.six samples of fermented milk preparations were examined for the presence of bifidobacteria. identification was based on fermentation tests, genetic relatedness studies and electrophoretic analysis. contrary to label information, bifidobacterium animalis was the only species present.19921556961
different electrophoretic patterns of cellular soluble proteins in bifidobacterium animalis.twenty-nine strains of bifidobacterium animalis, a species found only in animal habitats, were studied. strains from known origins such as rat, rabbit and chicken feces and strains isolated from extrabody environments such as sewage and fermented milk products were examined. the intestinal origins of strains isolated from sewage and fermented milk products were determined by means of the comparison of electrophoretograms of cellular soluble proteins. unknown origins of strains were recognized as ...19921556962
adhesion of bacteria to the cecal mucosal surface of conventional and germ-free chickens infected with eimeria tenella.when salmonella typhimurium and clostridium perfringens were tested in conventional chickens, larger numbers of s typhimurium and c perfringens adhered to eimeria tenella-infected ceca than to uninfected ceca. in germ-free chickens, s typhimurium and c perfringens adhered to the e tenella-infected cecal mucosa more than to the uninfected cecal mucosa, but fewer bacteroides vulgatus and bifidobacterium thermophilum adhered to the e tenella-infected ceca than to the uninfected ceca. many bacteria ...19921575383
antimutagenicity of milk cultured with lactic acid bacteria against n-methyl-n'-nitro-n-nitrosoguanidine.the antimutagenic effect of cultured milk using 71 strains of lactic acid bacteria belonging to the genus lactobacillus, streptococcus, lactococcus, and bifidobacterium on the mutagenicity of n-methyl-n'-nitro-n-nitrosoguanidine was investigated in vitro using salmonella typhimurium ta 100 as an indicator bacterium. each cultured milk sample displayed its characteristic antimutagenic effect on the mutagenicity of n-methyl-n'-nitro-n-nitrosoguanidine. the milk cultured with lactobacillus acidophi ...19921578035
[high-dose oral bacteria-therapy for chronic non-specific diarrhea of infancy].the authors evaluate the effectiveness of oral bacteriotherapy using a combination of anaerobe fecal lactobacilli for chronic non specific diarrhea of infancy. a double blind study was carried out in a total of 40 children treated with low and high doses of bacteria. the results confirm the importance of fecal flora in this disease and support the hypothesis that oral bacteriotherapy can improve clinical and laboratory presentation especially when given at high doses.19921579511
influence of decontamination on induction of arthritis in lewis rats by cell wall fragments of eubacterium aerofaciens. arthropathic properties of indigenous anaerobic bacteria.although the cause (or causes) of rheumatoid arthritis is unknown, many workers have suggested that microorganisms play a part. the intestinal flora in particular has been related to the development of joint inflammation. it has been shown previously that cell wall fragments of several anaerobic gram positive intestinal bacteria of human origin are arthritogenic after a single intraperitoneal injection in lewis rats. the part played by indigenous microflora in this model has now been studied by ...19921586251
influence of infant diets on the ecology of the intestinal tract of human flora-associated mice.the effect of diet on intestinal ecology was studied in germ-free mice that were inoculated orogastrically with predominant intestinal flora components isolated from the feces of breast-fed human infants. the flora components colonized the intestines of mice and persisted at fixed population levels. groups of flora- associated mice were fed either human milk, bovine milk, whey-dominant formula, or formula modifications exclusively for 2 weeks, and then examined for changes in small intestinal an ...19921593368
effect of bifidobacterium bifidum and lactobacillus acidophilus on gut mucosa and peripheral blood b 15 elderly individuals lyophilized bifidobacterium bifidum (bb) and lactobacillus acidophilus (la) (infloran) were administered in capsules (two capsules 4 times per day) for 28 days, while in 10 elderly controls placebo were given the same posology and for an equal period of time. the effects of this treatment on the immune system both at the periphery or the intestinal level were investigated. results show that bb and la significantly reduced the colonic inflammatory infiltration, without a ...19921597660
survival of bifidobacteria from human habitat in acidified milk.some industrial preparations from milk, such as yogurt, contain bifidobacteria as an additional probiotic element. the acidic environment of these products affects the viability of the bifidobacteria. the survival in acidic environment of one-hundred and ten bifidobacterial strains from human habitat was tested.19921602989
detection and identification of mobiluncus species by direct filter hybridization with an oligonucleotide probe complementary to rrna.a hybridization assay for direct detection and identification of mobiluncus species has been developed and tested. a [32p]-labelled synthetic oligonucleotide probe, complementary to a nucleotide sequence in the variable region v8 of mobiluncus 16s ribosomal rna, was utilized. one of the advantages of using rrna as target molecule for the hybridization assays is the copy number of rrna, which can be as high as 10(4), and that additionally three to six sites on the minus strand of the dna gene can ...19921642854
Displaying items 1 - 100 of 4016