the microbial metabolism of acetophenone. metabolism of acetophenone and some chloroacetophenones by an arthrobacter species.1. an organism that utilizes acetophenone as sole source of carbon and energy was isolated in pure culture and tentatively identified as an arthrobacter sp. 2. cell-free extracts of the acetophenone-grown organism contained an enzyme, acetophenone oxygenase, that catalysed an nadph-dependent consumption of o(2) in the presence of the growth substrate; approx. 1mol of o(2) and 1mol of nadph were consumed per mol of acetophenone oxidized. 3. cell-free extracts also contained an enzyme capable of t ...19754061
new microbial growth factor.a screening procedure was used to isolate from soil a penicillium sp., two bacterial isolates, and a streptomyces sp. that produced a new microbial growth factor. this factor was an absolute growth requirement for three soil bacteria. the penicillium sp. and one of the bacteria requiring the factor, an arthrobacter sp., were selected for more extensive study concerning the production and characteristics of the growth factor. it did not seem to be related to the siderochromes. it was not present ...1977327929
structural and immunochemical studies on d-arabino-d-mannans and d-mannans of mycobacterium tuberculosis and other mycobacterium species.serologically active d-arabino-d-mannas ([alpha]d, +82 degrees approximately 89 degrees; ratio of d-arabinose to d-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of m. tuberculosis, m. smegmatis, and several other mycobacterium species. these arabinomannans had similar structures, consisting of alpha-(1 leads to 5)-linked d-arabinose residues and alpha-(1 leads to 6)-, and (1 leads to 2)-linked d-mannose residues. methylation and enzymic degradation studies using ...1977413830
the fine structure of the branched alpha-d-glucan from the blue-green alga anacystis nidulans: comparison with other bacterial glycogens and phytoglycogen.the fine structure of the glycogen from the blue-green alga anacystis nidulans has been examined. after selective hydrolysis of all (1 yields 6)-alpha-d linkages by a bacterial isoamylase, the resulting mixture of linear chains was subjected to gel permeation chromatography. for purposes of comparison, the glycogens from escherichia coli and arthrobacter sp., amylopectin, phytoglycogen from sweet corn, and shell-fish glycogen were treated similarly. the profiles of the unit chains of a nidulans ...1975806344
facultative wood-digesting bacteria from the hind-gut of the termite reticulitermes hesperus.among the facultative bacteria capable of growth on mesquite wood which were isolated from the asceptically dissected hind-gut of the termite reticulitermes hesperus were two strains of bacillus cereus, one strain each of arthrobacter, alcaligenes and serratia, and a very small gram-negative fermentative rod. the b. cereus strains, the serratia marcescens strain and the arthrobacter sp. grew well on a mineral salts alpha-cellusose agar. one of the bacillus cereus strains and serratia marcescens ...1976822127
[degradation of 4-chlorobenzoic acid by an arthrobacter species (author's transl)].an arthrobacter sp. growing on 4-chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. protocatechuic acid is further attacked by "meta"-cleavage. during growth of the arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from the medium, suggesting the involvement of the "ortho"-cleavage pathway. the enzymes both for the "meta"- and the "ortho"-cleavage pathway are inducible.19761015950
pyrrolidine metabolism and its regulation in arthrobacter sp. 19751221030
cell growth and enzyme synthesis of a mutant of arthrobacter sp. (dsm 3747) used for the production of l-amino acids from d,l-5-monosubstituted hydantoins.a microorganism with the ability to form l-tryptophan from d,l-5-(3-indolyl-methyl)hydantoin (d,l-5-imh) was isolated and identified as arthrobacter sp. (dsm 3747). after isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high amounts of biomass with good specific activities concerning the enzymatic hydantoin-cleaving reactions. the ability of the microorganism to perform these bioconversions was found to b ...19901366910
production of l-tryptophan from d,l-5-indolylmethylhydantoin by resting cells of a mutant of arthrobacter species (dsm 3747).the reaction parameters and the stereospecificity of the enzymatic cleavage of d,l-5-indolylmethylhydantoin in producing l-tryptophan with resting cells of arthrobacter sp. dsm 3747 were studied. when intact cells were tested, the optimal ph was between 8.5 and 9.0 and the optimal temperature was 50 degrees c. both, l-n-carbamoylase and hydantoinase could be stabilized over 24 h at 30 and 40 degrees c by the addition of d,l-5-indolylmethylhydantoin. furthermore, the hydantoinase was stable over ...19901366911
microbial degradation of propoxur in turfgrass soil.this study was conducted to determine the degradation rates in turfgrass soil over a 12-month period after a single field application of propoxur and to isolate microorganisms from the soil capable of degrading the insecticide. soil samples were collected from a turfgrass experimental site near fort lauderdale, fl one week before the field application of propoxur, and over a 12-month period after the field application. mineralization rates in surface (0-15 cm depth) and subsurface (15-30 cm dept ...19921401729
[plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of arthrobacter].arthrobacter crysallopoietes strain km-4 degrading 2,6-dimethylpyridine and strain km-4a degrading both 2,6-dimethylpyridine and pyridine, arthrobacter sp. km-4b degrading 2,4-dimethylpyridine were isolated from soil. arthrobacter crystallopoietes km-4 and arthrobacter sp. km-4b contain 100 md plasmids pbs320 and pbs323. arthrobacter crystallopietes km-4a harbours a 100 md and 80 md plasmids. plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation ...19921454076
mineralization of 2-chloro- and 2,5-dichlorobiphenyl by pseudomonas sp. strain ucr2.pseudomonas sp. strain ucr2 was isolated from a multi-chemostat mating experiment between a chlorobenzoate-degrader, pseudomonas aeruginosa strain jb2, and a chlorobiphenyl-degrader, arthrobacter sp. strain b1barc. strain ucr2 differed from either of the parental organisms in that it grew on both 2-chloro- and 2,5-dichlorobiphenyl with concomitant release of chloride. phenotypic typing by the biolog system indicated that strain ucr2 shared greater similarity with strain jb2 (88%) than strain b1b ...19921459405
cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from arthrobacter sp. strain su.strains of arthrobacter catalyze a hydrolytic dehalogenation of 4-chlorobenzoate (4-cba) to p-hydroxybenzoate. the reaction requires atp and coenzyme a (coa), indicating activation of the substrate via a thioester, like that reported for pseudomonas sp. strain cbs3 (j. d. scholten, k.-h. chang, p. c. babbit, h. charest, m. sylvestre, and d. dunaway-mariano, science 253:182-185, 1991). the dehalogenase genes of arthrobacter sp. strain su were cloned and expressed in escherichia coli. analyses of ...19921476446
3,4-dihydroxyxanthone dioxygenase from arthrobacter sp. strain gfb100.bacterial extradiol ring-fission dioxygenases play a critical role in the transformation of multiring aromatic compounds to more readily biodegradable aromatic or aliphatic intermediates. arthrobacter sp. strain gfb100 utilizes an extradiol meta-fission dioxygenase, 3,4-dihydroxyxanthone dioxygenase (dhxd), in the catabolism of the three-ring oxygen heterocyclic compound xanthone. in this paper, we show that dhxd is a cytosolic enzyme, induced by growth on xanthone and maximally expressed during ...19911768091
substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries.benzene, toluene, and p-xylene (btx) were degraded by indigenous mixed cultures in sandy aquifer material and by two pure cultures isolated from the same site. although btx compounds have a similar chemical structure, the fate of individual btx compounds differed when the compounds were fed to each pure culture and mixed culture aquifer slurries. the identification of substrate interactions aided the understanding of this behavior. beneficial substrate interactions included enhanced degradation ...19911746958
bacteria that degrade p-chlorophenol isolated from a continuous culture system.two gram-positive coryneform bacteria that degraded p-chlorophenol isolated from a continuous culture system are characterized. isolate b (probably and arthrobacter sp.) completely removed the p-chlorophenol from a medium with a concomitant increase in cell density within 16 h. isolate f similarly removed the p-chlorophenol within 28 h but without an increase in cell density. isolates b and f also removed the p-chlorophenol from a medium with p-chlorophenol as the sole carbon source within 32 an ...19921581863
marine biosurfactants, ii. production and characterization of an anionic trehalose tetraester from the marine bacterium arthrobacter sp. ek 1.within a screening for biosurfactants we could isolate various n-alkanes utilizing marine bacteria which were capable of synthesizing glycolipids. one strain was identified as arthrobacter sp. ek 1 which produced trehalose lipids. after purification by column and thick layer chromatography the main fraction, an anionic 2,3,4,2'-trehalose tetraester, was obtained. the chain lengths of fatty acids ranged from 8 up to 14, furthermore succinate could be detected. since the place of substitution of s ...19911878107
isolation and characterization of a fluorene-degrading bacterium: identification of ring oxidation and ring fission arthrobacter sp. strain, f101, able to use fluorene as the sole source of carbon and energy, was isolated from sludge from an oil refinery wastewater treatment plant. during growth in the presence of fluorene, four major metabolites were detected and isolated by thin-layer chromatography and high-performance liquid chromatography. 9-fluorenol, 9h-fluoren-9-one, and 3,4-dihydrocoumarin were identified by uv spectra, mass spectrometry, and 300-mhz proton nuclear magnetic resonance. the fourth m ...19921444405
[biotransformation of zearalenone].the conversion of zearalenone by some strains of microorganisms was investigated. when the selective strains of rhodotorula sp., arthrobacter sp., saccharomyces sp., and candida sp. were incubated by shaking or standing at 28 degrees c for 72 h with an alcoholic solution of zearalenone as the substrate at a concentration of 2-10 mg/ml ethanol (50-100 micrograms/ml medium), it was readily converted to give zearalenols, consisting either mainly of the alpha-isomer (e.g. 96% in case of rhodotorula ...19921413733
crystal structure analysis, refinement and enzymatic reaction mechanism of n-carbamoylsarcosine amidohydrolase from arthrobacter sp. at 2.0 a resolution.n-carbamoylsarcosine amidohydrolase from arthrobacter sp., a tetramer of polypeptides with 264 amino acid residues each, has been crystallized and its structure solved and refined at 2.0 a resolution, to a crystallographic r-factor of 18.6%. the crystals employed in the analysis contain one tetramer of 116,000 m(r) in the asymmetric unit. the structure determination proceeded by multiple isomorphous replacement, followed by solvent-flattening and density averaging about the local diads within th ...19921381445
metabolism of carbamate insecticides by resting cells and cell-free preparations of a soil bacterium, arthrobacter sp. 19912032002
purification and some properties of beta-fructofuranosidase i from arthrobacter sp. k-1.arthrobacter sp. k-1, isolated from soil, produces beta-fructofuranosidase. this enzyme preparation was separated into three fractions (i, ii, and iii) by butyl-toyopearl 650 m column chromatography. the beta-fructofuranosidase i was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. the enzyme had a molecular weight of 52,000 by sds-polyacrylamide gel electrophoresis and 51,000 by gel filtration with ultrogel aca 44, and an isoelectric point of 4.3. the e ...19901368550
chlorinated biphenyl mineralization by individual populations and consortia of freshwater bacteria.comparative studies were performed to investigate the contribution of microbial consortia, individual microbial populations, and specific plasmids to chlorinated biphenyl biodegradation among microbial communities from a polychlorinated biphenyl-contaminated freshwater environment. a bacterial consortium, designated lps10, was shown to mineralize 4-chlorobiphenyl (4cb) and dehalogenate 4,4'-dichlorobiphenyl. the lps10 consortium involved three isolates: pseudomonas testosteroni (lps10a), which m ...19902117875
evidence for two distinct phosphonate-degrading enzymes (c-p lyases) in arthrobacter sp. glp-1.arthrobacter sp. glp-1 can utilize a wide range of organophosphonates as its sole source of phosphorus. the in-situ formation of sarcosine and methane from glyphosate and methanephosphonic acid respectively was studied. these two processes are differentially induced during phosphorus-deprivation. methanephosphonic acid strongly inhibits glyphosate degradation (i50 10 microm), but glyphosate has very little effect on methane generation (i50 150 mm). the pattern of inhibition by other organophosph ...19911368477
nadh production from nad+ with a formate dehydrogenase system involving immobilized cells of a methylotrophic arthrobacter strain.a convenient and economical method of nadh production from nad+ has been established using a formate dehydrogenase system involving immobilized cells of a methanol-utilizing bacterium. arthrobacter sp, km62. four kinds of cell entrapment were studied. an immobilized cell preparation showing a high nadh production activity was obtained by entrapment in a kappa-carrageenan gel lattice. the nadh-producing activity of the immobilized cells was investigated under various conditions. the nadh-producin ...19901366576
trimethyl lead degradation by free and immobilized cells of an arthrobacter sp. and by the wood decay fungus phaeolus schweinitzii.the continuing production of leaded petrol generates liquid wastes containing recalcitrant trialkyl lead, for which no suitable chemical treatment has been formulated. this investigation explores the feasibility of using microorganisms to catalyse the rate-limiting step of trimethyl lead degradation to dialkyl lead; this disproportionates chemically to give, ultimately, pb2+ which is treatable by classical methods. an arthrobacter sp. and a wood decay macrofungus, phaeolus schweinitzii provide n ...19901366364
oxidative degradation of squalene by arthrobacter organism isolated from soil and identified as arthrobacter sp. was studied for its squalene degradation. the degradation product from squalene, which accumulated in the culture broth, was isolated and identified as trans-geranylacetone by mass spectrometry, gas chromatography, infrared spectrometry, and nuclear magnetic resonance spectrometry. addition of a high concentration of k2hpo4 to the culture medium resulted in accumulation of fairly large amounts of carboxylic acids in addition to ge ...19751115507
4-keto-gamma-carotene(beta, psi-caroten-4-one), the major pigment of an arthrobacter sp. 19761000359
a new nad+-dependent opine dehydrogenase from arthrobacter sp. strain 1c.a new nad+-dependent opine dehydrogenase was purified to homogeneity from arthrobacter sp. strain 1c isolated from soil by an enrichment culture technique. the enzyme has a molecular weight of about 70,000 and consists of two identical subunits with molecular weights of about 36,000. the enzyme catalyzed a reversible oxidation-reduction reaction of opine-type secondary amine dicarboxylic acids. in the oxidative deamination reaction, the enzyme was active toward unusual opines, such as n-[1-r-(ca ...19892753861
soybean flower-to-seed movement of epiphytic bacteria.epiphytic tracer bacteris (a pseudomonas sp. and an arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. seed from two of these pods also carried tracers.1976943220
asni: a novel class ii restriction endonuclease from arthrobacter sp., strain n-cm, recognizing 5'-at/taat-3'.a new class ii restriction endonuclease, asni, with a novel sequence specificity was isolated from the gram-positive eubacterium arthrobacter species, strain n-cm. asni recognizes the unambiguously defined palindromic hexanucleotide (formula: see text) consisting of a- and t-residues. the novel enzyme in the presence of mg2+ cleaves specifically both strands as indicated by the arrows. the staggered cuts generate 5'-protruding ends with single-stranded 5'-ta-3' dinucleotide extensions. the novel ...19882841156
a host-vector system for an arthrobacter efficient host-vector system has been developed for an industrial strain of arthrobacter sp. (nrrl b3728)used for glucose isomerase production. protoplasts of arthrobacter were generated by treating the cells with 0.5 mg lysozyme ml(-1) for 60 min in a solution containing 0.5 m-sucrose. around 30% of the protoplasts regenerated on agar containing 0.5 m-sodium succinate as osmotic stabilizer. three hybrid vectors, pbl2100, pcg1100 and pcg2100, were constructed by combining the escherichia coli ...19882846755
structural characteristics of the corynebacterium lilium bacteriophage cl31.bacteriophage cl31 was isolated on a corynebacterium lilium strain. out of 30 strains tested, only cl31 was able to form plaques on corynebacterium glutamicum atcc 13287, brevibacterium lactofermentum atcc 21086, and arthrobacter sp. strain si55, but at a very low frequency. this phage belongs to group b of bradley's classification (d. e. bradley, bacteriol. rev. 31:230-314; 1967). its head is 53 nm in diameter, and its tail is 396 nm in length. the phage capsid contains three major proteins, of ...19873033280
nitrification in histosols: a potential role for the heterotrophic nitrifier.insufficient populations of nitrosomonas and nitrobacter were found in a pahokee muck soil (lithic medidaprit) to account for the nitrate concentration observed. to determine if heterotrophic nitrifiers could account for some of this discrepancy, a method was developed to measure the levels of heterotrophic nitrifiers in soil. a population of 4.1 x 10(5) arthrobacter per g of dry fallow soil, capable of producing nitrite and/or nitrate from reduced nitrogenous compounds, was observed. amendment ...1977869537
oxidation of linear terpenes and squalene variants by arthrobacter sp.cells of arthrobacter sp. that had been isolated from soil were used to study oxidation of some linear terpenes and squalene variants. the cells oxidized geraniol, nerol, and farnesol to the corresponding aldehydes, with partial conversion of the geometrical isomerism of the alpha,beta-double bond. the squalene variant, squalene-2,3-oxide, was cleaved to 9,10-epoxygeranylacetone and geranylacetone. squalene-2,3-22,23-dioxide was cleaved to 9,10-epoxygeranylacetone. these products were optically ...1977869527
effect of freezing and thawing on survival of three bacterial isolates from an arctic soil.three isolates, a pseudomonas sp., bacillus sp., and arthrobacter sp., which had been isolated from a meadow soil at devon island, canada, were subjected to freezing and thawing at low rates under various conditions. when cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and thaw rate. pseudomonas m216 was most susceptible to freeze-thaw damage under these conditions. arthrobacter m51 was the most resistant of the three isolates when frozen in sand or soi ...1978747810
effect of starvation on survival of three bacterial isolates from an arctic soil.three isolates, a pseudomonas sp., a bacillus sp. and an arthrobacter sp., which had been isolated from a meadow soil at devon island, canada, were subjected to starvation under varying conditions. the viabilities of the three isolates during starvation for 30 days in a carbon-free medium was assessed after the organisms had been grown continuously at varying rates in carbon- and nitrogen-limited media at 5 and 15 degrees c. pseudomonas m216 was the most resistant to starvation stress, bacillus ...1978747809
growth characteristics of three bacterial isolates from an arctic soil.three bacterial isolates, a pseudomonas sp., a bacillus sp., and an arthrobacter sp., commonly isolated from a hummocky sedge-moss meadow at devon island, n.w.t., canada, were selected for further taxonomic characterization and for a study of the effects of temperature and limiting carbon source on growth. pseudomonas m216 resembled p. putida and bacillus m153, b. carotarum. arthrobacter m51 had growth-factor requirements which were more complex than those of any named species of that genus. the ...1978688099
effect of oxygen on batch and continuous cultures of a nitrogen-fixing arthrobacter sp.growth, acetylene reduction, and respiration rate were studied in batch and continuous cultures of arthrobacter fluorescents at different oxygen partial pressures. the optimum po2 values for growth and acetylene reduction were 0.05 and 0.025 atm, respectively, but microorganisms can tolerate higher po2 values. the growth of cultures provided with combined nitrogen was dependent on oxygen availability, and strict anaerobic conditions did not support growth. acetylene reduction of a population gro ...1979476551
microbial desulfonation of substituted naphthalenesulfonic acids and benzenesulfonic acids.sulfur-limited batch enrichment cultures containing one of nine multisubstituted naphthalenesulfonates and an inoculum from sewage yielded several taxa of bacteria which could quantitatively utilize 19 sulfonated aromatic compounds as the sole sulfur source for growth. growth yields were about 4 kg of protein per mol of sulfur. specific degradation rates were about 4 to 14 mu kat/kg of protein. a pseudomonas sp., an arthrobacter sp., and an unidentified bacterium were examined. each desulfonated ...19873662502
transformation of morphine by resting cells and cell-free systems of arthrobacter sp.morphine can be transformed into 14-hydroxymorphine and a related unidentified material by resting cells of an arthrobacter species. cell-free extracts containing the transforming enzyme(s) have been obtained. o2, fe2+, and reduced nicotinamide adenine dinucleotide stimulate the transformation.1975240309
activation of the human complement cascade by bacterial cell walls, peptidoglycans, water-soluble peptidoglycan components, and synthetic muramylpeptides--studies on active components and structural requirements.cell walls isolated from 29 strains of 24 gram-positive bacterial species, whose peptidoglycans belong to the group a type of schleifer and kandler's classification, with one exception (arthrobacter sp.), were shown to activate the complement cascade in pooled fresh human serum mainly through the alternative pathway and partly through the classical one. the complement-activating effect of cell walls (5 species) possessing group b type peptidoglycan, except those of corynebacterium insidiosum, wa ...19873670125
emulsifier of arthrobacter rag-1: isolation and emulsifying properties.the oil-degrading arthrobacter sp. rag-1 produced an extracellular nondialyzable emulsifying agent when grown on hexadecane, ethanol, or acetate medium. the emulsifier was prepared by two procedures: (i) heptane extraction of the cell-free culture medium and (ii) precipitation with ammonium sulfate. a convenient assay was developed for measurement of emulsifier concentrations between 3 and 75 micrograms/ml. the rate of emulsion fromation was proportional to both hydrocarbon and emulsifier concen ...197936840
the catabolism of l-tyrosine by an arthrobacter arthrobacter sp. metabolizes l-tyrosine by a pathway involving 3,4-dihydroxyphenylacetate as a key intermediate. p-hydroxyphenylpyruvate is formed from tyrosine by an amino-transferase specifically requiring alpha-ketoglutarate for activity, and is then converted to p-hydroxyphenylacetate by an oxidative decarboxylation. p-hydroxyphenylacetaldehyde is not an intermediate in the formation of p-hydroxyphenylacetate. extracts of the bacterium oxidize 3,4-dihydroxyphenylacetate to delta-carboxyme ...197720216
molecular cloning and nucleotide sequencing of the arthrobacter dextranase gene and its expression in escherichia coli and streptococcus sanguis.a bacterial strain, which assimilated dextran and water-insoluble glucan produced by streptococcus mutans, was isolated from soil. the bacterium produced and secreted potent dextranase activity, which was identified as arthrobacter sp. and named cb-8. the dextranase was purified and some enzymatic properties were characterized. the enzyme efficiently decomposed the water-insoluble glucan as well as dextran. a gene library from the bacteria was constructed with escherichia coli, using plasmid puc ...19911859672
microbial decomposition of organism, which degrades alpha-picoline but also utilizes 2-ethylpyridine or piperidine as alternative growth substrates, has been isolated from soil and characterized as arthrobacter sp. alpha-picoline-grown cells oxidize 2-ethylpyridine and vice versa. other pyridine derivatives tested are neither utilized as growth substrates nor oxidized by the organism. alpha-picolinate and 2-hydroxy-6-methylpyridine are not metabolized, indicating that degradation is neither initiated by methyl oxidatio ...19744157133
-glutamyl-glutamic acid, an interpeptide bridge in the murein of some micrococci and arthrobacter sp. 19714256044
the stimulation of free radical formation in arthrobacter sp. by manganous ions. 19654285355
bacterial carotenoids. 38. c 50 -carotenoids. 9. isolation of decaprenoxanthin mono- and diglucoside from an arthrobacter sp. 19724635696
glucose utilization dependence on preferred carbon-source growth in an arthrobacter sp. 19734700353
deactivation of rg seed dressing by arthrobacter sp. 2b. 19734701337
2,4-dichlorophenoxyacetate metabolism by arthrobacter sp.: accumulation of a enzyme preparation from 2,4-dichlorophenoxyacetate-grown arthrobacter sp. converted cis,cis-2,4-dichloromuconate to chloromaleylacetate. the enzyme lactonizing the dichloromuconate to yield 2-chloro-4-carboxymethylene but-2-enolide was separated from the butenolide-delactonizing enzyme.19734751794
purification and characterization of haloalcohol dehalogenase from arthrobacter sp. strain enzyme capable of dehalogenating vicinal haloalcohols to their corresponding epoxides was purified from the 3-chloro-1,2-propanediol-utilizing bacterium arthrobacter sp. strain ad2. the inducible haloalcohol dehalogenase converted 1,3-dichloro-2-propanol, 3-chloro-1,2-propanediol, 1-chloro-2-propanol, and their brominated analogs, 2-bromoethanol, as well as chloroacetone and 1,3-dichloroacetone. the enzyme possessed no activity for epichlorohydrin (3-chloro-1,2-epoxypropane) or 2,3-dichloro-1 ...19911846134
mechanism of nitrification by arthrobacter sp.resting cells of arthrobacter sp. excrete as much as 60 mug of hydroxylamine-nitrogen per ml when supplied with ammonium. an organic carbon source in abundant supply is necessary for the oxidation. resting cells oxidize hydroxylamine to nitrite and 1-nitrosoethanol, the former accumulating only when an exogenous carbon source is available. cell-free extracts contain an enzyme catalyzing the formation of hydroxylamine from acetohydroxamic acid, a hydroxylamine-nitrite oxido-reductase, and an enzy ...19725030624
the isolation and characterization of glycogen from arthrobacter sp. nrrl b1973. 19655320421
cometabolism of m-chlorobenzoate by an arthrobacter.twenty isolates representing nine bacterial genera were obtained from enrichment cultures and were shown to cometabolize one or more of 22 substituted benzoates. one of the isolates, an arthrobacter sp., cometabolized m-chlorobenzoate to a product identified as 4-chlorocatechol by thin-layer chromatography and ultraviolet and infrared spectroscopy. the data indicate that cometabolism by the arthrobacter results from the formation of products by its benzoate-oxiding enzyme system that are not act ...19705480101
formation of 2,4-dichlorophenol and 2,4-dichloroanisole from 2,4-dichlorophen-oxyacetate by arthrobacter sp. 19676035146
occurrence and ultrastructural characterization of bacteria in association with and isolated from azolla caroliniana.the occurrence and ultrastructure of bacteria in leaf cavities of symbiotic azolla caroliniana were examined by transmission electron microscopy. bacteria were observed in all leaf cavities of azolla cultures. five ultrastructurally distinct types of bacteria were observed in each individual leaf cavity. features used to characterize the bacteria included morphology, cell wall structure, and cytoplasmic organization. at least one gram-positive and as many as four gram-negative types of bacteria ...19911785935
effect of microorganisms on the phytotoxicity of herbicides. vi. modification of venzar activity by bacterial cultures.interactions between two bacterial strains and venzar were compared. it was found that the mechanism of interactions is various and causes the modification of herbicide phytotoxicity. metabolites of bacillus subtilis 72 interfered with herbicide by affecting physiological processes in plant tissues and enhancing its inhibitory influence. arthrobacter sp. 18 strain decreased the phytoinhibitory effect of herbicide due to conjugation with the carrier from venzar.19816168180
marine biosurfactants, i. screening for biosurfactants among crude oil degrading marine microorganisms from the north sea.three bacterial strains of marine origin were isolated during a screening for biosurfactants among n-alkane degrading microorganisms. one strain-identified as alcaligenes sp. mm1-produced a novel glucose lipid. in the case of arthrobacter sp. ek 1 the well-known trehalose tetraester was found as major component. from another pure culture classified as arthrobacter sp. si 1, extracellular emulsifying agents with properties indicating high molecular weight substances were detected. furthermore tre ...19911878106
effect of bacterial extracellular polymers on the saturated hydraulic conductivity of sand columns.columns were packed with clean quartz sand, sterilized, and inoculated with different strains of bacteria, which multiplied within the sand at the expense of a continuous supply of fresh nutrient medium. the saturated hydraulic conductivity (hcsat) of the sand was monitored over time. among the four bacterial strains tested, one formed a capsule, one produced slime layers, and two did not produce any detectable exopolymers. the last two strains were nonmucoid variants of the first two. only one ...19921622240
description of the erythromycin-producing bacterium arthrobacter sp. strain nrrl b-3381 as aeromicrobium erythreum gen. nov., sp. nov.arthrobacter sp. strain nrrl b-3381t (t = type strain) is a nonmycelial, nonsporulating actinomycete that produces the macrolide antibiotic erythromycin. this bacterium differs in many ways from the type species of the genus arthrobacter (arthrobacter globiformis), suggesting that a taxonomic revision is appropriate. the g + c content of strain nrrl b-3381t dna is 71 to 73 mol%, and the peptidoglycan of this organism contains ll-diaminopimelic acid. evolutionary distance data obtained from 16s r ...19911883712
experimental apparatus for selection of adherent microorganisms under stringent growth conditions.a bioreactor apparatus is described for studying bacterial attachment. a cyclic, on-off, flow regime was imposed within the apparatus. model calculations illustrate the utility of this flow pattern in the selection and maintenance of slow-growing, adherent organisms. the apparatus is believed to have general utility in testing bacterial attachment influenced by many types of experimental or environmental constraints, including variations in fluid dynamics, presence of toxic substances (metals or ...19911892388
immunochemical properties of mannan-protein complex isolated from viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain by the action of zymolyase.viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain were treated with an arthrobacter sp. beta-1,3-glucanase, zymolyase-60,000, in the presence of a serine protease inhibitor, phenylmethylsulfonyl fluoride. fractionation of the solubilized materials with cetavlon (cetyltrimethylammonium bromide) yielded a purified mannan-protein complex, which had a molecular weight of ca. 150,000, approximately three times higher than that of the mannan isolated from the same cells by the hot-wate ...19846398395
mannopine and mannopinic acid as substrates for arthrobacter sp. strain mba209 and pseudomonas putida na513.the characteristics of mannopine and mannopinic acid utilization by agrobacterium tumefaciens b6s3, arthrobacter sp. strain mba209, and pseudomonas putida na513 were studied. strain b6s3 utilized the four mannityl opines, mannopine, mannopinic acid, agropine, and agropinic acid. it also utilized several mannityl opine analogs, which were modified in either the sugar or the amino acid moiety. it utilized mannopine more rapidly after preincubation on mannopine, mannopinic acid, or glutamine than a ...19911902209
microbial metabolism of quinoline and related compounds. vi. degradation of quinaldine by arthrobacter sp.quinaldine catabolism was investigated with the bacterial strain arthrobacter sp., which is able to grow aerobically in a mineral salt medium with quinaldine as sole source of carbon, nitrogen and energy. the following degradation products of quinaldine were isolated from the culture fluid and identified: 1h-4-oxoquinaldine, n-acetylisatic acid, n-acetylanthranilic acid, anthranilic acid, 3-hydroxy-n-acetylanthranilic acid and catechol. 3-hydroxy-n-acetylanthranilic acid was not further metaboli ...19902076195
[sulfur and iron cycling bacteria in low-sulfate meromictic lake kuznechikha].the hydrochemical characteristics, the composition of species and the localization of bacterial species involved in oxidation of sulfide and ferrous salts were studied in the meromictic lake kuznechikha with a low sulfate content in summer and in winter. the rate of bacterial sulfate reduction and the rates of bacterial and algal photosynthesis were determined using a radioisotope technique. the meromictic nature of the lake is due to the accumulation of ferrous salts (up to 212 mg/l fe2+) in th ...19806777648
involvement of plasmids in total degradation of chlorinated biphenyls.acinetobacter sp. strain p6 has previously been reported to utilize biphenyl (bp) and chlorinated bps, with accumulation of corresponding chlorobenzoic acids. arthrobacter sp. strain m5 was isolated as a contaminant in the culture of acinetobacter sp. strain p6 growing on 4-chlorobiphenyl and showed properties similar to p6 in the degradation of chlorinated bps. both strains harbored an identical plasmid of 53.7 megadaltons. these strains spontaneously lost the ability to utilize bp and 4-chloro ...19826814360
an examination of proton translocation and energy conservation during heterotrophic nitrification.whether selected heterotrophic nitrifiers, as do the autotrophs, conserve energy during the oxidation of their nitrogenous substrates was studied. the examination of proton translocation of four different bacterial nitrifiers capable of pyruvic oxime [(po), ch3-c(noh)-cooh] nitrification and by an nh4+ oxidizing arthrobacter sp. was initiated. three of the po nitrifying bacteria, all pseudomonads, oxidize hydroxylamine (nh2oh) at a greater rate than po and yielded only stoichiometric protons whe ...19902157622
utilization of acrylonitrile by bacteria isolated from petrochemical waste waters.a bacterium, utilising acrylonitrile as a sole source of carbon and nitrogen, was isolated from indian petrochemical corporation limited (ipcl) waste waters and identified as arthrobacter sp. this strain could also utilize acetonitrile, acetamide and acrylamide individually as a source of carbon and nitrogen. the metabolic studies with the whole cells indicated the sequential conversion of the nitrile to the respective amide and then to the respective acid and ammonia. the rate of nitrile hydrol ...19902279769
metabolism of glyphosate in an arthrobacter sp. glp-1.the metabolism of glyphosate [n-(phosphonomethyl)glycine] in a bacterium tentatively identified as an arthrobacter sp., capable of growth on this herbicide as its sole phosphorus source, has been investigated using solid-state nmr techniques as well as radiotracer analysis. the pathway involves the conversion of glyphosate to glycine, a c1 unit and phosphate. the phosphonomethyl carbon is specifically incorporated into the amino acids serine, cysteine, methionine, and histidine, as well as into ...19872439330
transformation of arthrobacter and studies on the transcription of the arthrobacter erma gene in streptomyces lividans and escherichia coli.we report the development of a plasmid-mediated transformation system for arthrobacter sp. nrrlb3381, using the streptomyces cloning vector pij702. our procedure gives a transformation frequency of 10(3)/micrograms of plasmid dna. in addition we have explored the expression of the arthrobacter erma gene in streptomyces lividans and escherichia coli, and shown that the erma promoter is recognized in s. lividans not e. coli. the relationship between arthrobacter, streptomyces and e. coli promoters ...19872443127
enzymatic dehalogenation of pentachlorophenol by extracts from arthrobacter sp. strain atcc 33790.arthrobacter sp. strain atcc 33790 was grown with pentachlorophenol (pcp) as the sole source of carbon and energy. crude extracts, which were prepared by disruption of the bacteria with a french pressure cell, showed no dehalogenating activity with pcp as the substrate. after sucrose density ultracentrifugation of the crude extract at 145,000 x g, various layers were found in the gradient. one yellow layer showed enzymatic conversion of pcp. one chloride ion was released per molecule of pcp. the ...19892793827
cloning and expression in escherichia coli of the gene for an arthrobacter beta-(1----3)-glucanase.when inserted in the correct orientation at the bamhi site of plasmid yrp7, an 8.6-kilobase bamhi fragment of arthrobacter sp. strain ycwd3 dna gave escherichia coli hb101 cells harboring the recombinant plasmid pbx20 the ability to lyse bakers' yeast cell walls or bakers' yeast glucan in agar medium. an extract of the transformed e. coli cells contained an endo-beta-(1----3)-glucanase with the same activity pattern as that of glucanase i produced by arthrobacter sp. strain ycwd3. although part ...19863096974
cometabolism of polychlorinated biphenyls: enhanced transformation of aroclor 1254 by growing bacterial cells.acinetobacter sp. strain p6 and a soil isolate, arthrobacter sp. strain b1b, were tested for their ability to transform aroclor 1254 as washed resting cells and as growing cells with biphenyl as the substrate. growing cells were far superior to resting-cell suspensions in terms of total polychlorinated biphenyl (pcb) transformation, transformation of specific pcb congeners, and diversity of congeners that were attacked. growing cells of acinetobacter sp. strain p6 and arthrobacter sp. strain b1b ...19883140725
three dehalogenases and physiological restraints in the biodegradation of haloalkanes by arthrobacter sp. strain ha1.arthrobacter sp. strain ha1 utilizes 18 c2-to-c8 1-haloalkanes for growth and synthesizes an inducible 1-bromoalkane debrominase of unknown physiological function (r. scholtz, t. leisinger, f. suter, and a.m. cook, j. bacteriol. 169:5016-5021, 1987) in addition to an inducible 1-chlorohexane halidohydrolase which dehalogenates some 50 substrates, including alpha, omega-dihaloalkanes. alpha, omega-dihaloalkanes were utilized by cultures of strain ha1 under certain conditions only. c9 and c8 homol ...19883223767
enzymatic dehalogenation of 4-chlorobenzoate by extracts from arthrobacter sp. su dsm extracts from arthrobacter sp. su dsm 20407 an enzyme was detectable, that converted 4-chlorobenzoate into 4-hydroxybenzoate. this conversion was also observed when no oxygen was present in the reaction mixture. boiling for 5 min destroyed the enzyme activity. 4-bromo- and 4-iodobenzoate were substrates for the enzyme too, but not 4-fluorobenzoate, 4-chlorophenylacetate and 4-chlorocinnamic acid. the enzyme showed optimum activity at 16 degrees c and at ph 7-7.5. the specific activity in the ...19883223988
induction of a light-inducible gene in arthrobacter sp. by exposure of cells to chelating agents and ph 5.transcription of a light-inducible gene in the prokaryote arthrobacter sp. is induced in the dark when cells are incubated with chelating agents or in medium at ph 5. however, repletion of metal ions such as ca2+, mn2+ or zn2+ or an increase in ph is required for accumulation of the gene product, an mr 21,000 polypeptide. but such changes in condition restore repression of the gene, and the decay in the rate of synthesis of the polypeptide follows the same time-course as when photodynamically in ...19883382667
isolation and characterization of an s-ethyl-n,n-dipropylthiocarbamate-degrading arthrobacter strain and evidence for plasmid-associated s-ethyl-n,n-dipropylthiocarbamate degradation.arthrobacter sp. strain te1 isolated from s-ethyl-n,n-dipropylthiocarbamate (eptc)-exposed soil degraded this herbicide effectively and could grow on eptc as the sole carbon source. te1 harboured four plasmids of 65.5, 60, 50.5, and 2.5 megadaltons. spontaneous mutants unable to degrade eptc arose at a high frequency, and this was further increased by treatment of the culture with acridine orange or incubation at high temperature. all eptc degradation-deficient (e-) mutants lacked the 50.5-megad ...19873606092
characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an arthrobacter sp.1-chlorohexane halidohydrolase from arthrobacter sp. strain ha1 was purified to homogeneity by fractional precipitation, ion-exchange chromatography, gel filtration, and high-performance liquid chromatography gel filtration. the enzyme was a monomer with a molecular weight of about 37,000; its amino acid composition and n-terminal sequence were determined. the enzyme had a broad optimum around ph 9.5, a temperature optimum near 50 degrees c, an activation energy of 40 kj/mol, and a molecular act ...19873667524
initial reactions of xanthone biodegradation by an arthrobacter sp.this study examined the catabolism of xanthone by an arthrobacter sp. (strain gfb100) capable of growth on xanthone as its main source of carbon and energy. an early catabolic intermediate was 3,4-dihydroxyxanthone. this compound was isolated from the growth medium of a mutant strain of the arthrobacter sp. which lacked the xanthone-inducible dihydroxyxanthone ring-fission dioxygenase of the wild-type strain. cell extracts from wild-type xanthone-grown cells oxidized 3,4-dihydroxyxanthone to a y ...19863745120
construction of a new host-vector system in arthrobacter sp. and cloning of the lipase gene.arthrobacter sp. strain mis38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from tn5) by an electroporation method. this shuttle vector is from brevibacterium lactofermentum and escherichia coli, pulrs8. the following optimal condition of electroporation was determined. a square wave pulse of 1 kv/cm electric field strength for 0.5 ms duration yielded 3 x 10(5) transformants/micrograms plasmid dna. the number of transformants increased with the amount ...19947765770
heterotrophic nitrifiction by arthrobacter sp.arthrobacter sp. isolated from sewage oxidized ammonium to hydroxylamine, a bound hydroxylamine compound, a hydroxamic acid, a substance presumed to be a primary nitro compound, nitrite, and nitrate. the concentration of free hydroxylamine-nitrogen reached 15 mug/ml. the identification of hydroxylamine was verified by mass spectrometric analysis of its benzophenone oxime derivative. the bound hydroxylamine was tentatively identified as 1-nitrosoethanol on the basis of its mass spectrum, chemical ...19725030623
production of glutamic acid by an arthrobacter sp. i. identification and nutritional requirement in relation to glutamic acid production. 19826188337
kinetic characterization of tyramine oxidase of arthrobacter species.amine oxidases (ec 1.4.3) represent a rather nebulous group of proteins with relative narrow substrate and inhibitor specificities. several different enzymes fall into these amine oxidase classes and the classification of some of them still remains ambiguous. kinetic and physico-chemical properties of tyramine oxidase of arthrobacter sp. were investigated to decide if this enzyme belongs to the flavin containing tyramine oxidase class (ec or if it is more related to another amine oxidas ...19948038724
roles of bacterial attachment and spontaneous partitioning in the biodegradation of naphthalene initially present in nonaqueous-phase liquids.the mineralization by an arthrobacter sp. of naphthalene initially dissolved in di(2-ethylhexyl)phthalate exhibited a slow phase followed by a rapid phase. triton x-100, which inhibited cell attachment, prevented the onset of the second phase. triton x-100 increased the extent of mineralization of naphthalene initially present in 2,2,4,4,6,8,8-heptamethylnonane. cells attached to the interface mineralized the aromatic hydrocarbon at a rate four times higher than the rate of partitioning in the a ...19948074534
interaction between bacterial metabolites and some pesticides. ii. change of phytotoxicity of the herbicide roneet by the phenolic metabolites of arthrobacter sp.the bacteria from arthrobacter genus isolated from sugar beet rhizosphere were found to produce gallic, protocatechuic, p-hydroxybenzoic, syryngic, vanillic, veratric acids, p-quinone and two unidentified phenolic compounds. the mixture of the bacterial phenolic compounds increased the phytotoxicity of roneet, inhibiting the germination of wheat. model experiments showed that the phenolic acids used with the herbicide roneet increased its phytotoxicity and p-hydroxybenzoic acid was found to be t ...19846209927
three different 2,3-dihydroxybiphenyl-1,2-dioxygenase genes in the gram-positive polychlorobiphenyl-degrading bacterium rhodococcus globerulus p6.rhodococcus globerulus p6 (previously designated acinetobacter sp. strain p6, arthrobacter sp. strain m5, and corynebacterium sp. strain mb1) is able to degrade a wide range of polychlorinated biphenyl (pcb) congeners. the genetic and biochemical analyses of the pcb catabolic pathway reported here have revealed the existence of a pcb gene cluster--bphbc1d--and two further bphc genes--bphc2 and bphc3--that encode three narrow-substrate-specificity enzymes (2,3-dihydroxybiphenyl dioxygenases) that ...19938335622
a sulfated polysaccharide produced by an arthrobacter species.a new sulfated polysaccharide was isolated from the culture supernatant of a strain of arthrobacter sp. the polysaccharide purified with quaternary ammonium salts consists of d-galactose, d-glucose, sulfate, phosphorus, glucosamine, muramic acid, alanine, glutamic acid, glycine, and ll-diaminopimelic acid in a molar ratio of 56 : 9.0 : 68 : 6.4 : 2.0 : 1.1 : 2.1 : 1.0 : 1.2 : 1.2. the presence of the two amino sugars and four amino acids suggests that the polysaccharide, which is principally a g ...19826298190
isolation of mannan-protein complexes from viable cells of saccharomyces cerevisiae x2180-1a wild type and saccharomyces cerevisiae x2180-1 a-5 mutant strains by the action of zymolyase-60,000.the viable whole cells of saccharomyces cerevisiae x2180-1a wild type and its mannan mutant strain s. cerevisiae x2180-1a-5, were treated with an arthrobacter sp. beta-1,3-glucanase in the presence of a serine protease inhibitor, phenyl-methylsulfonyl fluoride. fractionation of the solubilized materials of each strain with cetavlon (cetyltrimethylammonium bromide) yielded one mannan-protein complex. molecular weights of these complexes were almost the same as that of the mannoprotein of the muta ...19836355061
purification and properties of a nad-linked 1,2-propanediol dehydrogenase from propane-grown pseudomonas fluorescens nrrl b-1244.nad-dependent 1,2-propanediol dehydrogenase (ec activity was detected in cell-free crude extracts of various propane-grown bacteria. the enzyme activity was much lower in 1-propanol-grown cells than in propane-grown cells of pseudomonas fluorescens nrrl b-1244, indicating that the enzyme may be inducible by metabolites of propane subterminal oxidation. 1,2-propanediol dehydrogenase was purified from propane-grown ps. fluorescens nrrl b-1244. the purified enzyme fraction shows a single-p ...19836407398
cloning and characterization of a gene coding for the catechol 1,2-dioxygenase of arthrobacter sp. ma3.the cata gene, coding for the catechol 1,2-dioxygenase (c12o) of the bacterial strain arthrobacter sp. ma3, was cloned and expressed in escherichia coli. one plasmid containing a 6.1-kb ecori insert was selected by its ability to degrade catechol and to accumulate cis-cis-muconate. the dna insert of this plasmid was mapped with restriction enzymes. the cata gene was subcloned on a 1.3-kb psti-ecori fragment by deleting the adjacent restriction fragments. the nucleotide sequence of cata was deter ...19938423008
similarities in the induction of synthesis of a cell-surface polypeptide in arthrobacter sp. by near-uv irradiation and photodynamic conditions. 19836856688
metabolism of cyclohexaneacetic acid and cyclohexanebutyric acid by arthrobacter sp. strain ca1.a strain of arthrobacter was isolated by enrichment culture with cyclohexaneacetate as the sole source of carbon and grew with a doubling time of 4.2 h. in addition to growing with cyclohexaneacetate, the organism also grew with cyclohexanebutyrate at concentrations not above 0.05%, and with a variety of alicyclic ketones and alcohols. oxidation of cyclohexaneacetate proceeded through formation of the coenzyme a (coa) ester followed by initiation of a beta-oxidation cycle. beta-oxidation was blo ...19827076617
mechanism of enzymatic dehalogenation of pentachlorophenol by arthrobacter sp. strain atcc 33790.pentachlorophenol (pcp) dehalogenase from arthrobacter sp. strain atcc 33790 converts pcp to tetrachlorohydroquinone. in labeling experiments with h(2)18o or 18o2, only with h(2)18o was labeled product found. however, unlabeled tetrachlorohydroquinone became labeled after incubation with the enzyme in h(2)18o. therefore, distinction between an oxygenolytic or a hydrolytic dehalogenation mechanism for the pcp dehalogenase is not possible.19902254286
cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from arthrobacter sp. strain 1c.the gene coding for opine dehydrogenase from arthrobacter sp. strain 1c was cloned onto plasmid pbluescript ks(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. transformed escherichia coli cells overproduced nad+-dependent opine dehydrogenase under control of the promoter of the lac gene on pbluescript ks(-).19957487048
sarcosine oxidase contains a novel covalently bound fmn.sarcosine oxidase from corynebacterium sp. p-1 is a heterotetrameric protein containing three different enzymes: noncovalent fad, noncovalent nad+, and covalently bound flavin which is released as 8 alpha-(n3-histidyl)riboflavin upon complete hydrolysis of the protein. the following results show that the covalent flavin is not at the fad level, as previously proposed, but it is rather as 8 alpha-(n3- histidyl)fmn coenzyme. first, no amp is released when the protein moiety is treated with phospho ...19968611516
degradation of iprodione by a soil arthrobacter-like strain.a bacterial strain able to transform iprodione was isolated from a fast iprodione-degrading soil by enrichment procedures. transformation was detected through 3,5-dichloroaniline production as measured by a rapid colorimetric method. the strain, ma6, was tentatively identified as an arthrobacter sp. when it was incubated with ma6 in a minimum mineral medium (ph 6.5), iprodione (8.8 mumol/liter) was transformed into two major metabolites that were identified by high-performance liquid chromatogra ...19957574630
purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from arthrobacter sp. q36.a novel enzyme, maltooligosyl trehalose trehalohydrolase from arthrobacter sp. q36 was purified from a cell-free extract to an electrophoretically pure state by successive column chromatography on sepabeads fp-da13, butyl-toyopearl 650m, deae-toyopearl 650s, and toyopearl hw-55s. the enzyme specifically catalyzed the hydrolysis of the alpha-1,4-glucosidic linkage that bound the maltooligosyl and trehalose moieties of maltooligosyl trehalose. the km of the enzyme for maltosyl trehalose, maltotrio ...19958611745
cloning, sequence analysis, and expression of the flavobacterium pentachlorophenol-4-monooxygenase gene in escherichia coli.the pcpb gene of flavobacterium sp. strain atcc 39723 was cloned by using a degenerate primer designed from the n-terminal sequence of the purified enzyme. the nucleotide sequence of pcpb was determined and found to encode an open reading frame of 1,614 nucleotides, yielding a predicted translation product of 538 amino acids, in agreement with the estimated size of the purified protein analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the transcriptional start of pcpb was fo ...19937678243
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