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determination of the effective concentration of jodonal a for the disinfection of the skin and teats after milking.the bactericidal effectivity of jodonal a in 1:10, 1:5, and 1:3 solutions was tested on human skin and on the teats of cow mammary glands. the 1:3 dilution ratio proved best for three-minute exposure. jodonal a used in this concentration in 522 cows for the post-milking disinfection of teats for 10 months exerted no harmful effect on the skin of the mammary glands.197616377
devitalizing effect of jodonal a in vitro on bacteria subject to a short-term exposure.in a three-minute exposure in vitro jodonal a devitalized a culture of serological group b streptococci in a 2% concentration, staphylococcus aureus in a 16% concentration, pneumococcus in a 4.5% concentration, corynebacterium pyogenes in a 2.5% concentration, pseudomonas aeruginosa in a 3% concentration, and klebsiella pneumoniae in a 2% concentration. hence jodonal a concentrations higher than 16% should be tested for udder teat disinfection after the removal of teat cups.197616378
[changes in the serum proteins in suppurative surgical infection in cattle].studied were the serum proteins of 3 cattle with a spontaneous suppurative surgery infection and 10 cattle with an experimental infection of the same character. it was found that in such cases there is a rise in the total protein, the alpha and gamma globulins, and a drop in the albumin and the beta-globulins. the experimental suppurative infection caused by corynebacterium pyogenes produces changes of a higher degree in the protein spectrum than the experimental infection caused by str. pyogene ...197667698
studies on the occurrence of peptococcus indolicus and corynebacterium pyogenes in abscesses in swine, and on the occurrence of pc. indolicus in apparently normal skin and mucous membranes of piglets.pc. indolicus was isolated from 42.7% and cb. pyogenes from 48.7% of 150 pus-samples from abscesses in pigs (slaughter-house material, table i). in 41 specimens the two organisms were found together. further, pc. indolicus was demonstrated in 22.4% of 290 swabs from apparently normal skin and mucous membranes of piglets (autopsy material, table ii). by gel diffusion analysis the strains of pc. indolicus were referred to the serotypes b, c, d, e, or f. the type distribution (table iii) possibly r ...197898755
cross-reactions between mycobacteria. ii. crossed immunoelectrophoretic analysis of soluble antigens of bcg and comparison with other mycobacteria.cross-reactions between mycobacterium bovis bcg and various other mycobacteria, nocardia asteroides, corynebacterium pyogenes and listeria monocytogenes were studied by incorporating antibodies against these bacteria in the intermediate gel of a crossed immunoelectrophoretic system with bcg antigen and anti-bcg antibodies. in the bcg reference system forty-four distinct antigenic components were recorded, of which thiryt-three cross-reacted with mycobacterium tuberculosis, twenty-five with m. av ...1979106465
diagnosis of corynebacterium pyogenes infection in pigs by immunodiffusion test with protease antigen.a protease antigen was prepared from the culture supernatant of corynebacterium pyogenes by concentrating with a flash evaporator and ultrafiltration. it was adjusted to the concentration of 32 units by the single radial immunodiffusion with a tentative standard serum. in the immunodiffusion test, the antigen of 4 units reacted enough with sera having an antibody titer ranging from 1 to 128. as a result, it was decided that the antigen of 4 units should be used in the immunodiffusion test for th ...1979118384
[foot and mouth disease vaccination and corynebacterium pyogenes]. 1977194357
[etiology and immunoprophylaxy problems in sheep foot-rot (author's transl)]. 1979232438
aetiology of mastitis in nili-ravi buffaloes of pakistan. 1975239554
hemin-dependent growth stimulation and cytochrome synthesis in corynebacterium pyogenes.growth of corynebacterium pyogenes, an important pathogen in animals, was greatly increased on addition of hemin to a medium of tryptose plus mineral. the synthesis of a type b cytochrome in this organism appeared to depend on the presence of hemin in the growth medium.1977263823
[chronic tonsillitis probably caused by corynebacterium pyogenes var. hominis]. 1977321977
pathologic changes in the milk and udder of cows with mastitis. 1977326732
bovine mastitis produced by corynebacteria. 1977326735
[newer veterinary neuropathological conditions in south africa (author's transl)].a brief review is given of some of the more recent findings on lesions of diseases affecting the central nervous system of animals. these include local diseases caused by poisonous plants; helichrysum argyrosphaerum, solanum kwebense, matricaria nigellaefolia; infectious diseases e.g. heartwater, cerebral babesiosis and cranial abscesses in goats caused by corynebacterium pyogenes. reference is also made to some conditions, originally described in overseas countries and which subsequently have b ...1977327065
corynebacterium pyogenes mastitis among heifer calves.in august 1975 an outbreak of c pyogenes mastitis occurred involving 18 heifer calves at the national institute for research in dairying. this paper describes the outbreak which affected animals varying in age from five to 22 months. fourteen of the cases (77%) were among calves less than 10 months old. the advisability of using fly control measures among young stock in the summer months is discussed.1977327674
[laboratory studies of the urine in suppurative surgical infection in cattle].one hundred and eight cattle (20 controls, 14 with an experimentally induced and 74 with spontaneously occurred suppurative surgery infection) were studied in terms of their general condition and some of the urine indices--ph, specific weight, proteins, urobillinogen, acetone, sugar, blood, urea and sediment. it was established that when the general status deteriorated and death occurred the urine ph value rose (by 1--1.5), and the specific weight was 0.005--0.010 higher, while the level of urin ...1977331659
[the corynebacterium pyogenes infection in cattle. 1. incidence of the pathogen].reported are the incidence of corynebacterium pyogenes together with different pathological changes as well as the existence of latent corynebacterium pyogenes infections and their widespread occurrence. corynebacterium pyogenes was established from 609 in 2,130 samples of pathological processes, accounting for 28.6%. the pathogen was cultivated from various processes, including enlarged tail lymph nodes (61.1%), tail phlegmons (56.3%), abscesses (49.1%), epiphysiolyses (45.2%), liver abscesses ...1977336000
[the corynebacterium pyogenes infection of cattle. 2. tenacity of corynebacterium pyogenes].some common agents were tested for their effectiveness against corynebacterium pyogenes. the pathogen proved most susceptable to wofasteril. all germs were killed within ten minutes by a 0.005% solution. equally good action was recorded from all the other tested agents as well (lactic acid, lugol's solution, formalin, cupric sulphate, alcohol, and aethacridine. other studies were conducted with the view to testing the survival capacity of corynebacterium pyogenes in different media and storage c ...1977336001
[clinical and experimental studies of suppurative surgical infection in cattle].investigated were a total of 30 cattle 6 of which had experimental surgery infection and 24 showed spontaneously developing suppurative infection. the animals were studied in terms of general status, blood picture, quality of urine and synovia, and rumen content. the dynamis was followed up of the development of sepsis and purulent artritis. it was found that in case of suppurative surgical infections in cattle the general status was invariably affected, the severity of the process depending on ...1977343355
[comparative studies on the etiology of subclinical mastitis in cows of different breeds on farms]. 1977343359
corynebacterium pyogenes septic arthritis with plasma cell synovial infiltrate and monoclonal gammopathy.a chronic septic process developed in the right knee of an elderly man with advanced degenerative arthritis of both knees. open exploration, culture, and biopsy of the joint found that the pathogen was corynebacterium pyogenes and that the synovium was involved with a remarkable perivascular infiltrate of plasma cells. serum protein electrophoresis demonstrated a prominent m component. following antimicrobial therapy, the m protein level has gradually declined, and no evolution of multiple myelo ...1978348137
failure to induce in rabbits effective immunity to a mixed infection of fusobacterium necrophorum and corynebacterium pyogenes with a combined bacterin.failure to induce in rabbits effective immunity to a mixed infection of fusobacterium necrophorum and corynebacterium pyogenes with a combined bacterin. onderstepoort journal of veterinary research, 44 (4), 253--2;6 (1977). rabbits were immunized with alum-precipitated, oil adjuvant and an untreated bacterin composed of f. necrophorum and c. pyogenes. immunized rabbits were challenged intradermally with a mixture of f. necrophorum and c. pyrogenes. immunized rabbits were challenged intradermally ...1977349444
bacteriological examination of summermastitis secretions. the demonstration of bacteroidaceae.corynebacterium pyogenes was isolated from 22, peptococcus indolicus from 22, a microaerophilic coccus from 21, bacteroides melaninogenicus subsp. melaninogenicus from 18, fusobacterium necrophorum from 15, and various unidentified anaerobes from 9 of a total of 22 summermastitis secretions examined.1978351551
isolation of ureaplasma from bovine granular vulvitis.cultures for mycoplasmatales, viruses and bacteria were made from bovine vulvar swabs to determine whether ureaplasma was associated with a clinical granular vulvitis observed in 16 ontario dairy herds. ureaplasma was isolated from 23.5% of 34 clinically normal cows, 74% of 27 cows with mild to moderate vulvar hyperemia but no discharge and 100% of 20 cows with acute vulvar hyperemia accompanied by purulent discharge. there were statistically significant differences in rates of isolation among c ...1978352491
corynebacterium pyogenes endocarditis. report of a case with necropsy and review of the literature. 1978361919
[studies on the exotoxin of corynebacterium pyogenes following purification using gel filtration and deae ion exchanger]. 1978367011
[fibrous suppurative arthritis in sheep].described are cases of fibrinous supprative arthritis in sheep characterized by thickenning of the joint, resulting from the involvement of the capsule (periarthritis). histopathologically, there have been suppurative and necrotic processes combined with fibrinoid necrotizing of the collagen fibers and leukocyte infiltration of the joint capsule. slight leukocyte infiltration has also been said to occur in the parenchymal organs. microbiologic studies have revealed the presence of pyogenous bact ...1978369111
purification and some properties of hemolysin produced by corynebacterium pyogenes. 1979390193
a cystic jaw lesion in sheep. 1979390850
[contribution to an epidemiological study and the pathogenesis of caseous lymphadenitis of sheep (author's transl)]. 1979397037
[immunofluorescence tests for the detection of antibodies against corynebacterium pyogenes and streptococci in blood serum and vaginal mucus of cattle].bacteriological tests were applied to cattle with endometritis and vaginitis. included were cervical mucus samples and immunofluorescence tests to detect in that mucus as well as in blood serum antibody to corynebacterium pyogenes and streptococcus haemolyticus. the results pointed at intensive contact of the animals with the above pathogens and to their frequent occurrence in cervical mucus of cattle afflicted with endometritis and vaginitis. they also supported the assumption of localised anti ...1979398201
isolation of mycoplasmas from milk, lungs, and prepuce of cattle. 1979398242
precipitating antibody against protease of corynebacterium pyogenes in pigs.antibody in sera from pigs carrying an abscess associated with corynebacterium pyogenes and healthy pigs was examined by the agar gel diffusion test. in the test, the concentrated culture fluid containing the protease of c. pyogenes was used as antigen. as a result, precipitating antibody was demonstrated in sera from 25 of 30 abscessed pigs and a few of the healthy pigs. when the relationship between precipitating antibody and protease was examined by the immunoelectrophoresis and gel filtratio ...1977404574
feline pyothorax caused by a borrelia-like organism and corynebacterium pyogenes.a 2-year-old, neutered, male cat was euthanatized because of difficult repiration and inability to rise. necropsy revealed pyothorax. corynebacterium pyogenes was grown from the purulent thoracic exudate. a borrelia-like microorganism was demonstrated in the exudate, using phase-contrast microscopy.1979447585
experimental production of corynebacterium pyogenes abortion is sheep. 1979455990
[comparative clinical, bacteriological and pathohistological studies of the sex organs of infertile cows].investigations were performed with materials from 39 cows divided into two groups: i -- internal sexual organs of 29 cows slaughtered because of infertility; and ii -- biopsy material from 10 cows that showed symptomies sterility. the same material was studied microbiologically and histopathologically by routine methods. the material was taken from the cervix, the uterus, and the horns. bacteriologically, the presence was established of str. uberis. e. coli, str. dysgalactiae, staph. et albus et ...1979516444
effects of thin ewe syndrome on reproductive efficiency.a debilitating condition affecting mature ewes was found to be associated with the visceral form of caseous lymphadenitis, chronic progressive pneumonia, and corynebacterium pyogenes infections. the condition was termed thin ewe syndrome. affected and normal ewes were compared for reproductive efficiency by breed and age; differences in reproductive efficiency between affected and normal rambouillet and columbia ewes were highly significant (p less than 0.01). although not statistically signific ...1977604327
observation on the bacterial population of the os cervix of the ewe before and after embryo death.bacterial samples were isolated from the os cervix of mature merino ewes during a normal oestrous cycle and after colchicine treatment which killed the 25-day old embryos. the population of bacteria was small during the oestrous cycle and consisted of achromobacter spp. alcaligenes spp, corynebacterium spp, bacillus spp and escherichia coli. there was a significant increase in the numbers of bacteria isolated and a change in the proportions of the bacteria isolated from ewes after embryo death. ...1977612334
postpartum evaluation of bovine reproductive potential: comparison of findings from genital tract examination per rectum, uterine culture, and endometrial biopsy.the genital tract of 106 cows was evaluated after parturition by rectal palpation and by visual inspection of vaginal discharges. analysis of these results revealed a significant (p less than 0.01) correlation with the ratings of endometrial biopsies. the most frequent bacterial isolations included corynebacterium pyogenes, coliforms, or streptococci, or combinations thereof. abnormal ratings obtained by gross evaluation and by histologic examination were associated principally with c pyogenes. ...1978624671
echocardiographic diagnosis of endocarditis in a bull.a 2-year-old charolais bull was examined because of lameness that developed after infection of a tail laceration. a systolic murmur was auscultated during the routine physical examination. a diagnosis of endocarditis and polyarthritis secondary to septicemia from the tail lesion was made. the diagnosis was supported by echocardiography and blood cultures, which yielded corynebacterium pyogenes. the clinical diagnosis was confirmed at necropsy.1978659313
contribution to the taxonomy of haemolytic corynebacteria.in an attempt to assess the taxonomic relationships among human (corynebacterium haemolyticum), animal (corynebacterium pyogenes bovis) haemolytic corynebacteria, typical corynebacteria (corynebacterium diphteriae mitis, c. ovis, c. ulcerans) and group a and g streptococci, a number of biochemical parameters were established: the dna content of g + c, the presence of the cytochrome system, composition of fatty acids in free lipids and production of carboxylic acids as end products of fermentatio ...1978669489
[human diseases caused by corynebacterium pyogenes varians hominis]. 1976772516
studies on the occurrence of peptococcus indolicus and corynebacterium pyogenes in apparently healthy cattle. 1976773138
observations on experimental mastitis in mice with reference to summer mastitis in cattle. 1976773972
[clinical studies of an experimental suppurative surgical infection in cattle caused by cornynebacterium pyogenes].studied were clinically and paraclinically six cattle manifesting an experimental suppurative surgery infection caused by cornebacterium pyogenes, strain 1056. it was established that the infection assumes the course of septicaemia with metastases in the viscera, causing severe disturbances in the general condition and leading to death in most cases. found were also anemia, leukopenia, that alternated with leukocytosis presenting nuclear shift to left, changes in the erythrocyte sedimentation ra ...1976785801
antibody response to and immunity induced by corynebacterium pyogenes vaccine.a procedure is described for producing a high quality c. pyogenes vaccine. a satisfactory antibody response was obtained by administering 3 subcutaneous injections of vaccine at 10-day intervals, using doses of 2,0 ml for rabbits, 5,0 ml for sheep and 10,0 ml for cattle. the titres resulting from this procedure compared favourably with those obtained from following the more intensive schedule and higher doses of vaccine recommended by the british veterinary codex. the antibody response was at it ...1976796776
herd incidence of bovine mastitis in four danish dairy. districts ii. evaluation of the effect of the mastitis control scheme.bacteriological and cytological examinations have been carried out on samples from 6,808 cows belonging to 339 herds in four danish dairy districts. after a discussion of differences between the districts as regards intensivity of mastitis control, public health surveillance, etc., definitions are given of certain terms, such as presence of bacteria, infection, pathogenic versus non-pathogenic (less pathogenic) organisms. the rate of infection with pathogenic organisms varies between districts f ...1975807908
[etiology of dairy cows mastitis in slovakia in the years 1972-1974].in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples were higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae -- 15.77%, staphylococcus aureus -- 4.19%, other streptococci ...1975813355
[etiology of mastitis in dairy cows in slovakia during 1972-1974].in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples was higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae - 15.77%, staphylococcus aureus - 4.19%, other streptococci - 1. ...1975814671
activity of four cephalosporin antibiotics in vitro against bovine udder pathogens and pathogenic bacteria isolated from newborn calves.the in vitro activity of chephaloridine, cephalexin, cefatrizine (bl-s640), and cephapirin (bl-p-1322) was evaluated by the serial dilution method against pathogenic gram-positive and gram-negative bacteria isolated from bovine udders and neonatal calf diseases. cephapirin showed the comparatively greatest activity against the most common streptococcal species associated with bovine mastitis, whereas cephaloridine exhibited the best activity against staphylococcus aureus. cefatrizine was more ac ...1976816249
]acute severe mastitis in cows of the dutch-friesian breed (author's transl)].one hundred cases of severe acute mastitis are reported. bacteriological examination of the milk was negative in 23% of the cases. e. coli was most frequently found to be the causative organism (23%), followed by st. aureus (19%), c. pyogenes (10%), str. dysgalactiae (9%), str. agalactiae (5%) and a number of other pathogenic agents such as atypical streptococci, str. uberis, haemolytic streptococci, aerobacter aerogenes, kl. pneumoniae, a clostridium and p. multocida. in several cases, a tentat ...1976818734
thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa.acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, corynebacterium haemolyticum, corynebacterium pyogenes or from representatives of the genera arthrobacter, cellulomonas, curtobacterium, kurthia or oerskovia. thin-layer chromatography of whole-organism methanolysates ...1976825611
[inhibition of proteus sp. by nalidixic acid].the investigation comprised 192-positive and gram-negative strains of bacteria, including 111 proteus sp. the degree of resistance of these strains to nalidixic-acid was investigated on blood agar with 25 mug, 40 mug, and 50 mug nalidixin-acid per ml medium. none of the proteus strains were able to grow on medium with 50 mug nalidixic-acid per ml, whereas streptococcus sp., corynebacterium pyogenes, peptococcus indolicus, pseudomonas aeruginosa, and beta-toxic staphylococci all were found resist ...1976825831
[etiology of mastitidos in dairy cows in slovakia during 1975].in 1975, in the veterinary institutes in slovakia bacteriological examinations of 54824 samples of milk coming from 28737 dairy cows were performed. bacterial germs responsible for the inflammation of the mammary gland were found in the milk of 18.91% of cows. these were: streptococcus agalactiae (in 13.93% of cows), staphylococcus aureus (2.86%), other streptococci (1.32%), e. coli (0.13%), klebsiella sp. (0.13%), corynebacterium pyogenes (0.15%), other bacterial germs (0.39%). streptococci and ...1976828997
mycoplasma agalactiae subsp. bovis in pneumonia and arthritis of the bovine.the pneumonic lungs of 42 cattle from 26 feedlots were examined for the presence of mycoplasma, pathogenic bacteria and viruses. four animals representative of two lots failed to yield mycoplasma. one of these yielded the virus of infectious bovine rhinotracheitis and pasteurella hemolytica, the other yielded only p. p. multocida. nine animals in eight lots yielded mycoplasma sp.: five of these were m. bovirhinis, two were m. arginini and two were untypable. all of these animals yielded one or m ...1977832194
[occurrence and etiology for subclinical mastitis in cows].studied were a total of 16,571 cows on 89 farms by means of the bernburg test. milk was sampled from the positively reacting quarters of the udder by taking 18,047 samples intended for bacteriologic investigation. the demonstration of mastitis streptococci was carried out on "tkt" agar merk, of pathogenic staphylococci, hemolytic streptococci, and corinebacteria--on dextrose agar oxoid containing 7.5% citrated calf blood. the isolated hemolytic streptococcus bacteria from the two nutrient media ...1977898651
an outbreak of diphtheria in boergoat kids.during an outbreak of diphtheria or ulcerative stomatitis in young boergoat kids fusobacterium necrophorum was consistently isolated under anaerobic cultural conditions from material taken from the edges of the ulcerative lesions from all of the cases examined. corynebacterium pyogenes, staphylococcus aureus, actinobacillus lignieresi, moraxella spp. and lactobacillus spp. were also isolated anaerobically and/or aerobically. the typical symptoms and lesions were confirmed to the mouth, tongue an ...1976940095
bronchiectasis in yearling feedlot cattle.thoughout all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. from the 3,943 dead cattle, 1,988 necropsies were made; of the cattle necropsied, 32 (1.6%) had bronchiectasis. in this disease, the permanently dilated small bronchi and bronchioles, located in ventral parts of the lungs, were filled with accumulations of exudate and microorganisms, including pasteurella hemolytica, pasteurella multocida, corynebacterium pyogenes, escherichia coli, salmonella an ...1976956033
[corynebacterium pyogenes var. hominis infections]. 1976967454
[a hypophysis-abscess-syndrome in cattle. i. clinical investigations (author's transl)]. 19751105401
preweaning mortality in pigs. 6. incidence and causes of pneumonia. 19751105402
preweaning mortality in pigs. 7. polyarthritis.the incidence and causes of polyarthritis in sucking pigs were studied in herds with 2,936 litters during a 2-year period. among pigs more than 3 days old, the average morbidity rate was 3.3 per cent and the mortality rate amounted to 1.5 per cent (table i). among herds the morbidity ranged from 0.9 to 7.0 per cent and the mortality due to polyarthritis from 0.5 to 2.8 per cent. the incidences of polyarthritis were higher in the progeny of gilts than in the progeny of sows of other age groups (t ...19751105403
[sensitivity of mastitis pathogens to antibiotics and chemotherapeutic agents].sensitivity of mastitis bacteria to antibiotics and chemotherapeutic agents. the author tested the following bacteria, isolated from milk: -2236 strains of streptococci (1384 of group b, 262 of group c, 62 of group d, 109 of e, 16 of f, 24 of g, 7 of h, 11 of k, 270 of l, 5 of m, 41 of n, 45 of p); 361 ungroupable streptococci; 18,481 camp-positve, 1,966 camp-negative beta-haemolytic streptococci; 5,403 camp-negative, non-haemolytic streptococci; 1,179 strains of staphylococci, 596 of coliforms, ...19751106346
summer mastitis in heifers: studies on the seasonal occurrence of actinomyces pyogenes, peptostreptococcus indolicus and bacteroidaceae in clinically healthy cattle in denmark.with the aim of investigating the seasonal occurrence of actinomyces pyogenes, peptostreptococcus indolicus, bacteroides melaninogenicus ss. levii and fusobacterium necrophorum, and thus the potential for development of summer mastitis, clinically healthy danish holstein-friesian heifers due to calve in the autumn were sampled from the teat tip, the conjunctiva and the oral cavity at 2-6 week intervals from 1979 to 1981. the overall isolation rates of f. necrophorum, p. indolicus and b. melanino ...19921348381
comparative bacteriological studies on summer mastitis in grazing cattle and pyogenes mastitis in stabled cattle in denmark.a total of 143 secretions from clinical cases of summer mastitis (sm) in grazing cattle and from 89 cases of pyogenes mastitis (pm) in stabled cattle were examined bacteriologically. the typical bacteriological finding was a mixed flora in which the predominant organisms were actinomyces pyogenes (sm-70%, pm-85%), peptostreptococcus indolicus (54%, 54%), a microaerophilic coccus (stuart-schwan coccus) (26%, 25%), fusobacterium necrophorum biovar b (22%, 12%), bacteroides melaninogenicus (20%, 9% ...19921355316
phylogenetic evidence for the transfer of eubacterium suis to the genus actinomyces as actinomyces suis comb. nov.the 16s rrna primary structures of eubacterium suis dsm 20639t (t = type strain) and bifidobacterium bifidum dsm 20456t were determined by sequencing in vitro amplified rdna. sequence comparisons indicated that b. bifidum is moderately related to representatives of the genera actinomyces and mobiluncus. the closest relative of e. suis is actinomyces pyogenes. e. suis and a. pyogenes are more closely related phylogenetically to one another than to the other actinomyces species that have been inve ...19921371060
actinomyces pyogenes mastitis among lactating cows following foot-and-mouth disease. 19921413424
effect of intrauterine bacterial infusions and subsequent endometritis on prostaglandin f2 alpha metabolite concentrations in postpartum beef cows.multiparous angus and crossbred angus cows were used to determine the effect of induced endometritis on plasma concentrations of 13,14-dihydro-15-keto-prostaglandin f2 alpha (pgfm) and progesterone (p4) and on duration of the estrous cycle of treatment. beginning on the day of calving (d 0), blood samples were collected on alternate days. on three consecutive days, ranging from d 8 to 14 of the first postpartum estrous cycle, uterine horns were inoculated transcervically with either 3 x 10(9) co ...19921429292
evaluation of the rapid ana ii and api zym systems for identification of actinomyces species from clinical specimens.classification and identification of fermentative actinomycetes are labor-intensive and problematic. in this study, we evaluated the applicability and reliability of the rapid ana ii system (innovative diagnostic systems, inc., atlanta, ga.) and the discriminatory value of the api zym system (societes analytab products inc., la balme les grottes, france) in the identification of actinomyces-like bacteria by using conventional methods as a reference. eighty-five strains, including 71 isolates fro ...19921452693
chronic frontal sinusitis in dairy cattle: 12 cases (1978-1989).chronic frontal sinusitis in 12 dairy cattle most often was associated with a history of dehorning, in which the sinus was entered (67%), or with respiratory tract disease (25%). the most common organisms isolated were actinomyces pyogenes and pasteurella multocida. signs of infection did not develop for months in some cattle and were often intermittent. the most common clinical signs included anorexia, lethargy, fever, frontal bone distortion, exophthalmos, abnormal posture, nasal discharge, an ...19921500335
ultrasonographic study of early embryonic loss induced by actinomyces pyogenes in cattle.four cows were inoculated into the uterus with actinomyces pyogenes between 30 and 41 days of gestation. gross morphological changes were monitored by rectal palpation and with a realtime b-mode two-dimensional scanner with a 7.5 mhz transrectal linear transducer, shortly before infection and afterwards at three to 12 hours intervals. two control groups of cows 27 to 50 days pregnant were used: two cows were inoculated with 6 ml of sterile saline into the uterine lumen and four cows were treated ...19921502772
the bactericidal effect of n-acetyl-beta-d-glucosaminidase on bacteria.the bactericidal activity of n-acetyl-beta-d-glucosaminidase (nagase) on some of the potential bacterial pathogens of the cow was determined. nagase treatment significantly decreased the mean log10 number of actinomyces pyogenes (p less than 0.01) and staphylococcus aureus (p less than 0.05) after 2 and 4 hours of incubation at 37 degrees c. similarly nagase treatment significantly (p less than 0.05) reduced the mean log10 number of streptococcus agalactiae and pseudomonas aeruginosa after 4 hou ...19921514239
evaluation of the api coryne test system for identification of actinomyces pyogenes.the present study was designed to evaluate the accuracy of the api coryne test system for identification of actinomyces pyogenes. the test system correctly identified 36 of 42 a. pyogenes and 4 of 5 comparatively studied arcanobacterium haemolyticum-cultures. the biochemical profiles of the remaining 6 a. pyogenes- and 1 a. haemolyticum-cultures were not included in the analytical profile index. none of the cultures were misidentified. according to the api database (atb plus v 1.5.4.) the uniden ...19921519407
pyometra in camels: case report.pyometra was recorded in five camels and actinomyces pyogenes (corynebacterium pyogenes) was isolated from pus of the affected camels.19921551020
an investigation of bacterial causes of arthritis in slaughter hogs.joints from 153 arthritic and 80 normal slaughter hogs were examined by culture for presence of bacteria. although none of the normal joints yielded bacteria, 37% of the disease joints were positive for bacterial growth. of 67 bacterial isolates obtained, 45% were erysipelothrix rhusiopathiae. occurrence of other bacteria in order of their frequency was streptococcus suis (16%), actinomyces pyogenes (10%), mycoplasma spp. including 3 m. hyorhinis isolates (7%), staphylococci (7%), streptococcus ...19921554766
[actinomyces pyogenes: conventional and api system bacteriologic study of 103 strains isolated from ruminants].one hundred and three strains of actinomyces pyogenes isolated from ruminants were examined for morphological, cultural and biochemical properties by standard tests and by the api 50 ch and api coryne methods. no biotype could be demonstrated, but a few atypical non- or practically non-proteolytic strains were detected which should be differentiated from arcanobacterium haemolyticum. criteria for laboratory identification of a pyogenes were established: gram stain; culture on blood agar; deep ag ...19921610079
human infections with actinomyces pyogenes (corynebacterium pyogenes).actinomyces pyogenes (corynebacterium pyogenes), a well-known pathogen in many animals, was isolated from 11 danish patients since 1968. bacteriologic characteristics and clinical pictures of the patients are described. ability to hydrolyze gelatine, to produce beta-glucuronidase, to reach with antisera against group-g streptococci, and to produce acid from xylose differentiates a. pyogenes from arcanobacterium haemolyticum, with which it has at times been confused. actinomyces pyogenes is an es ...19921611850
cell surface hydrophobicity of actinomyces pyogenes determined by hexadecane adherence- and salt aggregation studies.cell surface hydrophobicities of actinomyces pyogenes were determined by measuring the adherence of the bacteria to hexadecane droplets and by salt aggregation tests. among 42 a. pyogenes cultures tested 25 (60%) adhered strongly (adherence greater than or equal to 75%) and 17 (40%) less pronounced (adherence between 25-75%) to the hexadecane droplets. pre-treatment of the bacteria with proteolytic enzymes completely eliminated the adherence properties whereas heat treatment had no effect. the s ...19921621475
[results of studies with aborted cattle fetuses].the laboratory of the animal health service of south holland investigated 2410 cases of spontaneous abortion (twins (112) were considered one case). seventy-eight afterbirths without foetuses were also examined. the aim of the study was not only to find the cause of abortion but also to see whether there was a relation between the results of the different bacteriological, virological and histological tests used. the presence of igg in the blood of foetuses older than 5 months was also determined ...19921626316
evaluation of a scheme for predicting the gram-staining reaction of organisms causing bovine mastitis.the accuracy of a scheme for predicting the gram-staining reaction of organisms causing bovine mastitis in cows with systemic signs of disease (anorexia) was evaluated over 1 year. criteria for making the predictions included: season of year, stage of lactation, appearance of milk, detection and duration of teat injuries, and milk odor. it was possible to determine the cause by microbiologic culture of specimens from 136 of the 147 cows of the study. of 78 infections caused by gram-negative (mos ...19901689287
[pyomyositis].pyomyositis (pm) is characterized by bacterial infection and suppuration of striated muscle. it is very rarely encountered in temperate climates. only five cases have hitherto been reported in denmark. two cases of pm are presented. both of these demonstrate the difficulty of establishing early diagnosis and the severe complications of pm.19921738956
studies on the possible role of cattle nuisance flies, especially hydrotaea irritans, in the transmission of summer mastitis in denmark.the summer mastitis pathogens actinomyces pyogenes, peptococcus indolicus, bacteroides melaninogenicus ss. levii, fusobacterium necrophorum and streptococcus dysgalactiae were isolated from the polyphagous symbovine dipterans hydrotaea irritans (fallén) and morellia sp. caught around dairy heifers on pasture, but not from the haematophagous species haematobia irritans (l.), haematobosca stimulans (meigen), culicoides sp. and simulium sp. secretions from clinical cases of summer mastitis proved t ...19911773120
characterization of 2 groups of actinomyces-like bacteria isolated from purulent lesions in pigs.two groups of actinomyces-like bacteria isolated from purulent lesions in pigs, were characterized and compared with actinomyces pyogenes of porcine origin. one group showed characteristics which differed in many respects from a. pyogenes but which corresponded more closely to those of the non-official species 'actinomyces suis'. certain carbohydrate reactions differed from those given in earlier descriptions. this group was isolated only from purulent vaginal discharge and aborted foeti. the ot ...19911789014
an abattoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. iv. bacteriological findings in chronic pneumonic lesions.a total of 855 pig lungs were collected at slaughter and evaluated macroscopically. bacteriological examinations were carried out on tissue samples from chronic pleuropneumonic lesions (n = 196) and from chronic bronchopneumonic lesions with suppuration (n = 14). samples from normal lung tissue (n = 22) were also included. pasteurella multocida was isolated from 54%, actinobacillus (haemophilus) pleuropneumoniae from 11%, and streptococcus spp. from 14% of the pneumonic lesions, respectively. fr ...19911814189
bacteriological investigations of clinical mastitis in heifers in sweden.bacterial analyses were carried out of 2069 udder secretions, isolated from 1481 heifers with mastitis in eight veterinary districts in sweden. streptococci, e.g. streptococcus dysgalactiae and str. uberis, dominated the bacterial flora, being isolated from 34.4 and 19.5% respectively of heifers with clinical mastitis occurring from puberty up to 14 d post partum. bacterial species generally regarded as important pathogens in the summer mastitis complex, e.g. actinomyces pyogenes, stuart-schwan ...19911856352
bacterial flora of the uterus of cows after calving on two hygienically contrasting farms.intrauterine swabs were obtained from cows after calving on two commercial dairy herds with contrasting hygienic environments and incidence of leucorrhea, and cultured aerobically and anaerobically. of 26 cows with a normal calving and puerperium, eight of 14 (57 per cent) were sterile on farm b where hygiene was poor, compared with five of 12 (42 per cent) on farm a where hygiene was better. two cows on farm b retained their placentas and subsequently developed metritis/endometritis. actinomyce ...19911858270
n-acetyl-beta-d-glucosaminidase, etiologic agent, and duration of clinical signs for sequential episodes of chronic clinical mastitis in dairy cows.this study examined effects of repeated episodes of clinical mastitis in chronically infected quarters on milk n-acetyl-beta-d-glucosaminidase activity and duration of clinical signs. milk samples were collected at each clinical onset from 49 chronic mastitis cases on a 1700-cow michigan dairy farm. there were 49 first episodes of clinical mastitis, 49 second episodes, and 13 episodes of third or more. agents isolated were staphylococcus aureus (18.4%), staphylococcus (7.3%), no growth (20.2%), ...19911880262
endometrial biopsy in holstein-friesian dairy cows. iii. bacteriological analysis and correlations with histological findings.this study examines the results of bacterial culture from 159 endometrial biopsy samples from 97 commercial dairy cows and correlations between bacteriological and histological findings. bacteria were isolated from approximately 80% of biopsies taken at day 26 and day 40 postpartum. eleven percent of biopsies were positive for both aerobic and anaerobic culture. streptococci, escherichia coli and actinomyces pyogenes were the most common isolates. isolation of a. pyogenes from a biopsy at day 26 ...19911884297
early abortion in cattle induced by experimental intrauterine infection with pure cultures of actinomyces pyogenes.actinomyces pyogenes from a case of endometritis was used to study the effects of infection of the bovine embryo between days 27 and 41 of pregnancy. from 10(9) to 10(10) washed organisms were introduced into the uterine lumen of four pregnant cows. two pregnant cows were inoculated with sterile saline and four pregnant cows were treated with cloprostenol. embryonic death and abortion followed 29 to 144 hours after the inoculation of the live bacteria. the aborted embryos were macerated or clear ...19911897106
cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods.cellular fatty acid (cfa) compositions of 561 asporogenous, aerobic gram-positive rods were analyzed by gas-liquid chromatography as an adjunct to their identification when grown on blood agar at 35 degrees c. the organisms could be divided into two groups. in the first group (branched-chain type), which included coryneform cdc groups a-3, a-4, and a-5; some strains of b-1 and b-3; "corynebacterium aquaticum"; brevibacterium liquefaciens; rothia dentocariosa; and listeria spp., the rods had siza ...19911899679
further observations on enhancement of the infectivity of fusobacterium necrophorum by other bacteria.it had already been shown with a single virulent strain (a42) of fusobacterium necrophorum that suspension of the fusobacteria in sub-lethal doses of broth cultures of other bacteria reduced the minimum infective dose (greater than 10(6) organisms) for mice by subcutaneous inoculation, sometimes to less than 10 organisms. the present study extended the known range of bacteria with strong infectivity-enhancing properties to include bacillus cereus, klebsiella oxytoca and staphylococcus aureus; an ...19911902184
necrosis and sloughing of skin associated with limb cellulitis in four cows and a calf: predisposing causes, treatment and prognosis.four cows and a calf with non-suppurative limb cellulitis were observed subsequently to suffer skin necrosis and sloughing in the affected limbs, either on or distal to the metacarpus or metatarsus. in comparison with six cows with suppurative corynebacterium pyogenes limb cellulitis, topical therapy or the cases with skin necrosis and sloughing was adequate and the prognosis was good, when compared with the rigorous systemic therapy applied to the cows with suppurative cellulitis, some of which ...19911957470
summer mastitis in heifers: a bacteriological examination of secretions from clinical cases of summer mastitis in denmark.a total of 166 samples of secretions collected from danish heifers suffering from clinically diagnosed summer mastitis were examined bacteriologically. one hundred and sixty three samples yielded positive findings whereas no growth was obtained from 3 specimens. the majority of samples revealed a mixed flora in which the predominant components were actinomyces pyogenes, peptostreptococcus indolicus, a microaerophilic coccus (stuart-schwan coccus), fusobacterium necrophorum, bacteriodes melaninog ...19901973009
radiological case of the month. cavitary pneumonia due to arcanobacterium hemolytic. 19911994689
effect of damage to the teat end on the experimental induction of mastitis in dry cows with corynebacterium pyogenes.the teat ends of 12 dry cows were contaminated with corynebacterium pyogenes. to determine whether a pre-existing (an)aerobic bacterial infection of the udder was a predisposing factor for a c pyogenes mastitis they included infected and uninfected quarters. anaerobic bacteria could not be found and mastitis was not induced. when the teats were contaminated with c pyogenes after the teat ends had been injured 30 of the quarters became infected, and anaerobic bacteria were demonstrated in many qu ...19912003353
epizootic of external and internal abscesses in a large goat herd over a 16-year period.from 1973 through 1988, 518 external and internal abscesses were diagnosed in a large commercial goat herd. of the 518 abscesses, 238 were primary and 280 were secondary abscesses in the same or other anatomic locations. during this period, the herd varied in size from 94 goats in 1973 to 431 goats in 1988. abscesses in the jaw area were detected in 141 goats, 4 of which developed osteomyelitis of the mandible. sternal abscesses were detected in 72 goats, with 2 also having osteomyelitis of the ...19912004986
a sensitive method for isolating fusobacterium necrophorum from faeces.the isolation of fusobacterium necrophorum present in small numbers in heavily contaminated material such as faeces or soil is hampered by the lack of an efficient selective medium and by the high minimum infective dose of the organism. a sensitive method for the detection and isolation of faecal strains of f. necrophorum type a was based on the subcutaneous injection of faeces, suspended (5% w/v) in broth culture of actinomyces (corynebacterium) pyogenes or staphylococcus aureus to increase fus ...19912019300
aerobic bacteria from nasal discharge of goats suffering from clinical ppr: isolation and identification.bacteriological isolation of aerobic bacteria from nasal discharge of achondroplastic west african dwarf goats showing clinical signs of peste des petits ruminants (ppr), and the laboratory procedures carried out for the identification of the isolates are described. from a total of forty nasal swabs obtained seventy five isolates were recovered. the percentage frequency of isolation of different organisms in descending order of occurrence is as follows: staphylococcus 44.0%; streptococcus 22.67% ...19912023545
characteristics of actinomyces pyogenes involved in lameness of male turkeys in north-central united states.a new condition of clinical lameness in 20 male turkey flocks of north-central united states, associated with isolation of gram-positive rod bacteria from lesions of osteomyelitis, is characterized. the characterization confirmed the randomly selected isolates as actinomyces pyogenes based on macroscopic and microscopic observations and 17 biochemical tests. the disease was reproduced within 3 weeks in all male turkeys, following an intravenous challenge at 15 weeks of age. the agar gel precipit ...19912029253
fusobacterium necrophorum hemolysin in bovine hepatic abscess.an immunofluorescence study was made on bovine hepatic abscess containing fusobacterium necrophorum predominantly. the abscess section stained with anti f. necrophorum hemolysin serum demonstrated fluorescence which formed irregular and granular shapes. actinomyces pyogenes isolates from the abscess were not stained with the serum. these findings suggest that the bacterial hemolysin contributes to the formation of the hepatic abscess during an infection with f. necrophorum.19902082629
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