| lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes. | the respiratory quinones of 73 strains of gram-positive bacteria including spore-forming rods, lactic-acid bacteria and actinomyctes were examined. menaquinones with seven isoprenoid units (mk-7) were the main quinone type found in representatives of the genus bacillus and in sporolactobacillus inulinus. however, a strain of b. thuringiensis produced mk-8 in addition to mk-7, and strains of b. lentus and b. pantothenticus appeared to produce mk-9 and mk-8, respectively, with no mk-7. in the clos ... | 1979 | 119033 |
| [actinomyces odontolyticus, first study in france]. | the authors report the detailed study of a strain of actinomyces odontolyticus isolated from a pleural liquid. gas-liquid chromatography can specify the biochemistral metabolism of the germ and set off an endocellular lipidolytic activity. | 1977 | 417834 |
| actinomycotic pulmonary abscess in an immunosuppressed patient. | the clinical history of a patient with rheumatoid arthritis and bacteriologic findings from a pulmonary abscess occurring during prednisone therapy are presented. direct transthoracic aspiration of the lesion yielded a pure culture of actinomyces odontolyticus. this is believed to be the first case of a deep visceral infection with this organism, an inhabitant of the normal mouth and gums. immunosuppression of the host probably played a role in establishment of the infection. | 1979 | 495569 |
| malar mass due to actinomyces odontolyticus. | actinomyces odontolyticus was isolated from a patient with a soft tissue mass in the malar region. the organism was identified on the basis of morphological, cultural, and biochemical characteristics. on histological examination, the tissue mass contained several granulomatous foci with small, basophilic staining areas resembling microscopic sulfur granules. this is believed to be the first reported case of actinomycosis due to a. odontolyticus. | 1977 | 886003 |
| bacteriocin from actinomyces odontolyticus with temperature-dependent killing properties. | a strain of actinomyces odontolyticus, originally isolated from human dental plaque, produced a non-dialyzable, trypsin-sensitive substance that was bactericidal for certain strains of bifidobacteria at 42 degrees c but not at 37 degrees c. detectable quantities of the bacteriocin were not produced in liquid media. experimentally useful yields were obtained by extraction from pour plate cultures of producer cells. at 42 degrees c, exponential killing did not occur until indicator cells had doubl ... | 1977 | 907331 |
| a case of empyema thoracis caused by actinomycosis. | a female patient who presented with left empyema thoracis caused by actinomyces odontolyticus is reported. she responded to treatment with penicillin and metronidazole but after 3 weeks developed leucopenia complicated by gram-negative septicaemia. leucopenia improved rapidly on withdrawal of metronidazole. treatment was continued with a prolonged course of penicillin and she made an uneventful recovery. | 1992 | 1303486 |
| evaluation of the rapid ana ii and api zym systems for identification of actinomyces species from clinical specimens. | classification and identification of fermentative actinomycetes are labor-intensive and problematic. in this study, we evaluated the applicability and reliability of the rapid ana ii system (innovative diagnostic systems, inc., atlanta, ga.) and the discriminatory value of the api zym system (societes analytab products inc., la balme les grottes, france) in the identification of actinomyces-like bacteria by using conventional methods as a reference. eighty-five strains, including 71 isolates fro ... | 1992 | 1452693 |
| the endodontic microflora revisited. | the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ... | 1992 | 1479923 |
| binding of actinomyces viscosus to collagen: association with the type 1 fimbrial adhesin. | treatment of hydroxyapatite (ha) with human type i or type iii collagen strongly promoted adhesion of actinomyces viscosus ly7 cells. treatment with human type v collagen was somewhat less effective while treatment with human type iv or rat type i collagen was significantly less effective. electron microscopic observations revealed that a. viscosus cells also attached to fibrils prepared from human type i collagen. the alpha 1 (1) polypeptide chain derived from type i collagen was ineffective in ... | 1991 | 1682868 |
| use of monoclonal antibodies with neutralizing effects on toxic antigens from human bacterial plaque to detect specific bacteria by colony blotting. | inflammatory periodontal diseases are provoked by bacteria which adhere to teeth at the gingival margin and form plaques containing toxins detectable by their effect on mammalian cells in culture. the aim of this study was to make toxin-neutralizing monoclonal antibodies and determine whether they detect antigen in specific oral bacteria. bacterial plaque was collected from teeth and homogenized, and the fluid phase (plaque extract) was boiled or first fractionated over sephacryl s-300. hybridom ... | 1991 | 1757553 |
| tetrameric manganese superoxide dismutases from anaerobic actinomyces. | superoxide dismutase was isolated from each of the anaerobically grown organisms actinomyces naeslundii, actinomyces strain e1s.25d, and actinomyces odontolyticus. the enzymes were 100,000-110,000 mol wt acidic proteins (pi 4.3-4.6) and contained mn and zn, but no detectable fe. the mn and zn content varied with the enzyme source. a. naeslundii superoxide dismutase, specific activity 2200 u/mg, contained 2.3 g atoms mn and 1.4 g atoms zn per mole tetramer whereas a. odontolyticus sod, specific a ... | 1990 | 2112898 |
| changes in subgingival microbiota during puberty. a 4-year longitudinal study. | it was the purpose of the present investigation to monitor the composition of the subgingival microbiota at selected sites in individuals passing through puberty and to correlate observed changes with the development of pubertal maturation. between the ages of 11 and 14 years, pubertal and skeletal maturation was monitored annually in 22 boys and 20 girls. during this time, subgingival microbial samples were taken every 4th to 5th month (10 times in 4 years) mesially of the upper first molars. h ... | 1990 | 2262580 |
| keratinolytic activity of cutaneous and oral bacteria. | a test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (ph 7.2) with 1 mm dithiothreitol. the degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. several oral and cutaneous bacteria were investigated: bacteroides gingivalis, bacteroides intermedius, treponema dent ... | 1987 | 2434427 |
| an in vitro study of the antibacterial and antifungal properties of an irreversible hydrocolloid impression material impregnated with disinfectant. | the difficulties associated with the disinfection of alginate (irreversible hydrocolloid) materials have resulted in the development of an irreversible hydrocolloid impression material impregnated with an antimicrobial compound, didecyldimethyl ammonium chloride. this investigation assessed the antibacterial and antifungal properties in vitro of this innovative irreversible hydrocolloid material by using the agar well technique. the microorganisms used were pseudomonas aeruginosa, streptococcus ... | 1989 | 2514263 |
| the microflora associated with the development of initial enamel decalcification below orthodontic bands in vivo in children living in a fluoridated-water area. | thirty-four caries-free teeth destined for orthodontic extraction were banded to provide a protected area for the accumulation of plaque. the teeth were extracted at one, two, four, eight, and 14 days after being banded, and samples of the flora below the band were analyzed for the presence of streptococcus sanguis, streptococcus 'mitior', 'mutans streptococci', actinomyces viscosus, actinomyces naeslundii, actinomyces odontolyticus, lactobacillus species, and veillonella. after plaque sampling, ... | 1989 | 2600252 |
| subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance. | hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ... | 1989 | 2704977 |
| human salivary acidic proline-rich proteins and statherin promote the attachment of actinomyces viscosus ly7 to apatitic surfaces. | actinomyces viscosus ly7 cells adsorbed in high numbers to experimental pellicles formed on hydroxyapatite (ha) from human parotid or submandibular saliva but not to pellicles prepared from human plasma or serum. to determine the nature of the salivary components responsible for promoting adhesion, pellicles were prepared from fractions of submandibular and parotid saliva obtained by chromatography on trisacryl gf 2000 columns. adsorption of ly7 cells was promoted by two groups of fractions. eac ... | 1988 | 2892794 |
| intergeneric bacterial coaggregations involving mutans streptococci and oral actinomyces. | mutans streptococci (ms) representing eight different serotypes were tested for their ability to coaggregate in vitro with oral actinomyces and other streptococcal species. of the mutans streptococci tested, only strains of s. cricetus (formerly s. mutans serotype a) displayed pronounced coaggregations and only with certain strains of actinomyces. s. cricetus coaggregated, by lactose nonreversible mechanisms, with serotype 4 actinomyces naeslundii wvu963 and wvu924 and with serotype 2 actinomyce ... | 1987 | 3117688 |
| the comparative cytotoxicity of periodontal bacteria. | the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ... | 1988 | 3204481 |
| thoracic, abdominal, and vertebral actinomycosis. diagnosis and long-term therapy in three dogs. | the clinical, laboratory, radiographic, and histologic features and the response to therapy in three dogs with actinomycosis are reported. one dog (dog 1) had a 12-cm nonresectable mass extending from the ventrolateral chest wall into the left ventricular myocardium. another dog (dog 2) had a diffuse peritonitis with "sulfur granules" and two large masses. one of these masses was nonresectable involving adjacent abdominal structures. a third dog (dog 3) had a subvertebral mass at t1-3 producing ... | 1988 | 3230558 |
| the synergistic effect of cefotaxime and desacetylcefotaxime against clinical isolates of anaerobic bacteria. | the synergistic interaction of cefotaxime and desacetylcefotaxime against 187 clinically significant anaerobic organisms was investigated. fusobacterium nucleatum, actinomyces odontolyticus, propionibacteria, lactobacilli, peptostreptococci, streptococcus intermedius and veillonella were sensitive to cefotaxime. both eubacterium lentum and streptococcus morbillorum were resistant. the susceptibility of the clostridia varied from 0.125 to greater than 256 mg/l; only 20% of species demonstrated sy ... | 1988 | 3396488 |
| inhibition of [3h]-thymidine uptake in human gingival fibroblasts by extracts from human dental plaque, oral bacteria of the streptococcus and actinomyces species. | extracts from human dental plaque, streptococcus mutans, streptococcus salivarius, streptococcus faecalis, streptococcus sanguis, actinomyces israelli and actinomyces odontolyticus inhibited [3h]-thymidine uptake by primary human gingival fibroblast cell lines as previous work has shown in respect of hela cells. the results show that a variety of oral bacteria, not usually considered to be periodontal pathogens, elaborate factors that adversely affect fibroblasts. | 1985 | 3857891 |
| the comparative cariogenicity and plaque-forming ability in vivo of four species of the bacterium actinomyces in gnotobiotic rats. | gnotobiotic wag/rij rats on a high sucrose diet were monoinfected with 12 strains of actinomyces spp. moderate levels of caries were induced by a single strain of actinomyces naeslundii and low levels by two strains of actinomyces viscosus, three strains of a. naeslundii and one strain of actinomyces israelii. no caries was induced by single strains of a. viscosus and a. israelii or by three actinomyces odontolyticus strains. only fissure caries was observed. scanning electron microscopy showed ... | 1985 | 3868974 |
| enterocutaneous fistulization due to actinomyces odontolyticus. report of a case. | a case of abdominal actinomycosis originating from the sigmoid colon is presented. actinomyces odontolyticus was isolated; it is a rarity, but should be suspected in the case of a palpable mass and several fistulas developing after a latent period of weeks to months following surgical or inflammatory trauma. the diagnosis is made by anaerobic cultivation. primary treatment with large doses of penicillin for several weeks may be supplemented by surgery. the strain isolated in the present case was ... | 1985 | 4017816 |
| actinomyces odontolyticus infections: review of six patients. | | 1985 | 4056447 |
| letter: systemic actinomycosis caused by actinomyces odontolyticus. | | 1974 | 4420083 |
| two new serological groups of actinomyces. | actinomyces odontolyticus and a. viscosus are designated as group e and group f in the serological grouping of the actinomyces. | 1970 | 4912526 |
| [pathogenic role of actinomyces apart from actinomycosis. apropos of 2 cases of acute suppurations with actinomyces odontolyticus]. | the authors report two cases of acute suppurative lesions due to actinomyces odontolyticus. they point out the fairly high incidence of this pathogen in such conditions, either isolated or more often as part of a mixed flora. conversely, actinomyces odontolyticus is only exceptionally recovered in true actinomycosis. | 1983 | 6320394 |
| development and evaluation of a coculture technique for identification of anaerobic organisms. | we investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. the biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. the standard reactions were compared with those obtained by preparing similar media, modified by containing no cysteine or resazurin, under aerobic conditions. preincubation of the modified b ... | 1984 | 6365960 |
| actinomyces odontolyticus isolated from the female genital tract. | actinomyces odontolyticus was isolated from genital tract specimens from 4.8% of 561 women fitted with intrauterine contraceptive devices and from 4% of 101 women with pelvic inflammatory disease and 1.8% of 525 women without pelvic inflammatory disease who were not known to be intrauterine contraceptive device wearers. the strains were isolated by prolonged anaerobic incubation of blood agar, with or without added 5% metronidazole or 1% neomycin. a odontolyticus has not been previously reported ... | 1984 | 6511983 |
| predominant cultivable microflora of plaque on removable dentures in patients with healthy oral mucosa. | plaque from the fitting surface of upper full dentures in eight patients with healthy palatal mucosa was studied. to characterize the predominant cultivable flora, 916 isolates (100-128 from each sample) were subcultured from anaerobic roll-tubes. streptococci constituted 0-81 per cent (median, 41 per cent) of the isolates with varying proportions of streptococcus milleri, streptoccus mutans, streptococcus salivarius, streptococcus mitior and streptococcus sanguis. staphylococcus aureus made up ... | 1983 | 6579900 |
| [fusobacterium necrophorum associated with actinomyces odontolyticus septicemia ]. | the authors have observed a case of fusobacterium necrophorum associate with actinomyces odontolyticus septicemia, which appears to be the first case reported. in a 19 year old man, 15 blood cultures allowed to isolate both germs. clinical manifestations were: acute-like abdomen, encephalitis, myocarditis and pleural effusion. treatment with amoxicillin and metronidazole was successful. | 1982 | 6750527 |
| bacteriology of experimental gingivitis in young adult humans. | from replicate trials of experimental gingivitis in four periodontally healthy subjects, 166 bacterial species and subspecies were detected among 3,034 randomly selected isolates from 96 samples. of these bacteria, actinomyces naeslundii (serotype iii and phenotypically similar strains that were unreactive with available antisera), actinomyces odontolyticus (serotype i and phenotypically similar strains that were unreactive with available antisera), fusobacterium nucleatum, lactobacillus species ... | 1982 | 7141708 |
| hepatic actinomycosis presenting as repeated cholestatic reactions. | a patient with hepatic actinomycosis caused by actinomyces odontolyticus is presented. the clinical course was characterized by 2 yr of episodic abdominal pains and fever together with marked acute elevations of serum alkaline phosphatase. the diagnosis escaped extensive laboratory studies, 2 liver biopsies and 1 laparotomy and was finally verified at a second laparotomy by histology and a positive culture. histopathology showed multiple small abscesses. no primary focus of infection was found. ... | 1982 | 7146831 |
| bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use. | the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ... | 1995 | 7548575 |
| carbohydrate depletion of immunoglobulin a1 by oral species of gram-positive rods. | bacterial deglycosylation of immunoglobulin al (iga1), the dominant isotype of antibody in the oral cavity, probably provides both nutrition as well as protection to the oral bacterial community. representative strains of oral gram-positive rods were tested for their ability to remove carbohydrates from iga1. detection of sialic acids was performed by means of high-performance liquid chromatography (hplc) separation (aminex hpx-87h) and ultraviolet light absorption at 190 nm, and neutral carbohy ... | 1994 | 7870470 |
| bilateral pulmonary infiltrates in association with disseminated actinomycosis. | the most common infectious cause of bilateral upper-lobe pulmonary disease is tuberculosis. however, we recently encountered a patient with bilateral apical infiltrates and multiple soft-tissue abscesses caused by actinomyces odontolyticus. other findings included fever, weight loss, and leukocytosis, and the patient's only known source of immunosuppression was a long history of alcoholism. there was no history of diabetes, steroid use, or other chronic underlying disease. the diagnosis was made ... | 1994 | 7948516 |
| [thoracic actinomycosis]. | thoracic actinomycosis is an rare disease in our medium. this fact, together with the variability of its forms of presentation and the difficulty in isolating its etiologic agent, make its diagnosis, particularly difficult. | 1993 | 8260516 |
| bacteriologic findings in peritonsillar abscesses in young adults. | aspirated pus samples from 124 patients with peritonsillar abscess were cultured quantitatively for aerobes and anaerobes. a total of 98% of the samples yielded bacteria. of the 550 isolates obtained (mean, 4.4 per patient), 143 were aerobes (representing 16 species or groups) and 407 were anaerobes (representing 40 species or groups). aerobes were isolated from 86% of patients-alone in 20 cases and together with anaerobes in 87. the most common aerobic isolates were streptococcus pyogenes (isol ... | 1993 | 8324134 |
| peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report. | peritonsillar abscess is a potentially life-threatening complication of acute tonsillitis. on occasion, peritonsillar abscess can extend to neck spaces and/or to the mediastinum. we describe a case of a patient with a peritonsillar abscess that extended to the neck, producing bilateral retropharyngeal abscesses and myonecrosis of the strap muscles. culture of a specimen of the necrotic muscle yielded prevotella intermedia, prevotella buccae, lactobacillus catenaforme, another lactobacillus speci ... | 1993 | 8324135 |
| [pulmonary actinomycosis: caused by actinomyces odontolyticus?]. | primary pulmonary actinomycosis is a purulent bronchopulmonary disease mimicking tuberculosis. the histological lesions contain characteristic yellow granules. the organism most frequently responsible for the disease is actinomyces israeli. we report the first case to our knowledge where actinomyces odontolyticus seems to be involved. | 1993 | 8378641 |
| coaggregation of prevotella intermedia with oral actinomyces species. | five strains of prevotella intermedia were examined for their ability to coaggregate with various gram-positive and gram-negative species of oral bacteria. two of the p. intermedia strains coaggregated with selected actinomyces species, p. intermedia 27 with actinomyces viscosus t14v and actinomyces naeslundii atcc 12104, pk606, pk984, and pk947, and p. intermedia 113 with actinomyces odontolyticus wvu 1546 and actinomyces israelii wvu 838. exposure of both prevotella strains but not the actinom ... | 1993 | 8478088 |
| comparison of the distribution of actinomyces in dental plaque on inserted enamel and natural tooth surfaces in periodontal health and disease. | the distribution of actinomyces naeslundii, actinomyces viscosus and actinomyces odontolyticus in healthy and diseased adult populations was studied in 3 different ways. first, supragingival plaque formation at 2 through 72 h was examined in 12 periodontally healthy adults using a removable pre-measured surface of enamel bonded to molars and premolars. second, a cross-sectional examination of the composition of both supragingival and subgingival plaque of unknown age was conducted in 205 adults ... | 1993 | 8510984 |
| actinomyces odontolyticus thoracopulmonary infections. two cases in lung and heart-lung transplant recipients and a review of the literature. | we present the first case of mediastinitis and the third case of pneumonia attributed to actinomyces odontolyticus. the first patient presented 10 months after single-lung transplant with a subacute apical infiltrate in the native lung and responded to therapy with oral penicillin. the second patient developed pyogenic mediastinitis 25 days after a heart-lung transplant and required sternal debridement and intravenous penicillin. we also review the literature on thoracopulmonary infections due t ... | 1996 | 8635341 |
| systemic antimicrobial treatment and guided tissue regeneration. clinical and microbiological effects in furcation defects. | the purpose of this investigation was to study the microbiota associated with furcation-involved teeth before and after treatment by the guided tissue regeneration procedure (gtr) with non-resorbable eptfe membranes, and to evaluate the benefit of additional systemic antimicrobial therapy (ornidazole). each of 10 patients contributed 1 pair of bilateral mandibular molars with comparable furcation defects. 5 defects were treated with a membrane and the active drug, 5 were treated without a membra ... | 1996 | 8739172 |
| polymicrobial brain abscess involving haemophilus paraphrophilus and actinomyces odontolyticus. | a case of brain abscess involving haemophilus paraphrophilus and actinomyces odontolyticus is presented. this combination of organisms has not previously been described. all brain abscess specimens should routinely be processed rapidly and cultured for a prolonged period to ensure recovery of fastidious organisms which may have implications for antibiotic therapy. | 1996 | 8761504 |
| rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests. | eighty-nine species of subgingival bacteria, represented by 121 reference strains and 892 patient isolates, including gram-negative, gram-positive, aerobic, facultatively anaerobic, microaerophilic, and anaerobic species, were characterized with a panel of fluorogenic, 4-methylumbelliferyl-linked substrate tests. identifications of all patient isolates were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins relative to reference strains. char ... | 1996 | 8789019 |
| periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants. | the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ... | 1997 | 9100195 |
| empyema necessitatis due to actinomyces odontolyticus. | | 1997 | 9106610 |
| [mandibular actinomycosis caused by actinomyces odontolyticus]. | | 1997 | 9147518 |
| transfection of actinomyces spp. by genomic dna of bacteriophages from human dental plaque. | bacteriophages that produced turbid or clear zones of lysis in strains of actinomyces were isolated from 22 of 124 samples of fresh human dental plaque. all human and nonhuman strains of actinomyces viscosus or actinomyces naeslundii tested in this study were sensitive to infection by one or more of these phages. in contrast, none of the actinomyces odontolyticus, actinomyces israelii, or actinomyces bovis strains tested were susceptible. results of restriction endonuclease analyses indicated th ... | 1997 | 9169205 |
| microbial complexes in subgingival plaque. | it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ... | 1998 | 9495612 |
| actinomyces naeslundii displays variant fimp and fima fimbrial subunit genes corresponding to different types of acidic proline-rich protein and beta-linked galactosamine binding specificity. | actinomyces naeslundii genospecies 1 and 2 bind to acidic proline-rich proteins (aprps) and statherin via type 1 fimbriae and to beta-linked galactosamine (galnacbeta) structures via type 2 fimbriae. in addition, a. naeslundii displays two types of binding specificity for both aprps-statherin and galnacbeta, while actinomyces odontolyticus binds to unknown structures. to study the molecular basis for these binding specificities, dna fragments spanning the entire or central portions of fimp (type ... | 1998 | 9712794 |
| oral microbiota and implant type membranes. | candida albicans (ca), staphylococcus aureus (sa), streptococcus sanguis (ss), actinomyces naeslundii (an), actinomyces odontolyticus (ao), porphyromona spp (p spp), candida glabrata (cg), candida krusei (ck), and rhodotorula spp (r spp) were tested with equal pieces of biodegradable membranes. membranes pretreated with saliva or clorhexidine and nontreated control membranes were tested in three different culture media containing 0.1 ml homologous suspension for each strain under study. incubati ... | 1998 | 9759038 |
| actinomyces naeslundii genospecies 1 and 2 express different binding specificities to n-acetyl-beta-d-galactosamine, whereas actinomyces odontolyticus expresses a different binding specificity in colonizing the human mouth. | a total of 102 strains of actinomyces were isolated from teeth, buccal mucosa and tongue in eight individuals. the isolates were characterized by multivariate statistical analyses of phenotypic characteristics, serotyping and binding to beta-linked galactosamine (n-acetyl-beta-d-galactosamine) and acidic proline-rich protein structures. based on these characteristics, isolates were classified into three major groups: (i) isolates of actinomyces naeslundii genospecies 2 were the dominant species ... | 1998 | 9872107 |
| dominant cross-reactive antibodies generated during the response to a variety of oral bacterial species detect phosphorylcholine. | the intraperitoneal immunization of balb/c mice with subgingival plaque from advanced periodontal pockets or with certain strains of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, actinomyces israelii, streptococcus mitis, or streptococcus oralis yielded frequently indistinguishable igm monoclonal antibodies which were reactive with antigens from a variety of oral bacteria. this study aimed to characterize the specificity of such monoclonal antibodies and the diversity of oral ba ... | 1999 | 10065949 |
| prevalence of nim genes in anaerobic/facultative anaerobic bacteria isolated in south africa. | this study investigated the prevalence of nim genes (proposed to encode a 5-nitroimidazole resistance product) in 64 anaerobic/facultative anaerobic bacteria. employing universal nim gene primers, 458-bp amplified fragments were recorded as presumptive positives in 22/64 strains at an annealing temperature of 52 degrees c and 15/64 strains at 62 degrees c, of which seven were propionibacteria. dna sequencing confirmed the presence of nima genes in propionibacterium spp. (five strains), actinomyc ... | 1999 | 10079531 |
| ribotype diversity of actinomyces with similar intraoral tropism but different types of n-acetyl-beta-d-galactosamine binding specificity. | sixty-three isolates of actinomyces naeslundii genospecies 1 and 2 and actinomyces odontolyticus from three subjects clustered into 22 ribotypes. unique ribotypes were found in the subjects and within individual tissue sites (bucca, tooth and tongue). a odontolyticus ribotypes shared tongue-specific binding properties, while those of genospecies 1 and 2 from buccal and tooth surfaces shared different types of n-acetyl-beta-d-galactosamine binding specificity. | 1998 | 10093534 |
| an evaluation of the co2 laser for endodontic disinfection. | the goal of this study was to evaluate the bactericidal action of the co2 laser on animal teeth infected with an endodontic bacterial species. after instrumentation, 24 freshly extracted incisors were inoculated with a known concentration of actinomyces odontolyticus and incubated anaerobically for 18 h. the incisors were separated into three groups: group 1--untreated control teeth; group 2--teeth treated with 3% naocl; and group 3--teeth lased with a co2 laser at 5 w using three successive 9.9 ... | 1999 | 10204466 |
| case of pleuropericardial disease caused by actinomyces odontolyticus that resulted in cardiac tamponade. | | 1999 | 10433600 |
| actinomyces odontolyticus pulmonary infection. | | 1999 | 10492328 |
| the effect of oral decontamination with clindamycin palmitate on the incidence of bacteremia after esophageal dilation: a prospective trial. | antibiotic prophylaxis to prevent bacterial endocarditis is recommended in high-risk patients undergoing esophageal dilation, a high-risk procedure. some studies suggest that the oropharynx is the source of bacteremia. a topical antibiotic mouthwash, which reduces bacterial colonization of the oral flora, might decrease bacteremia rates and would be an attractive alternative to systemic administration of antibiotics. | 1999 | 10502166 |
| an evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria. | the aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: facultative aerobes-anaerobes: candida albicans atcc 10231; enterococcus faecalis atcc 29212; escherichia coli atcc 25,922; pseudomonas aeruginosa atcc 27,853; streptococcus mitis atcc 9811; streptococcus mutans atcc 35668; streptococcus salivarius atcc 13419; and streptococcus sanguis atcc 10556. microaerophiles: actinobacillus actinom ... | 1999 | 10530260 |
| activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes. | the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ... | 1999 | 10543754 |
| activities of telithromycin (hmr 3647, ru 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and other antimicrobial agents against unusual anaerobes. | the comparative activity of telithromycin (hmr 3647) against 419 human anaerobic isolates was determined by the agar dilution method. at concentrations of </=0.5 microgram/ml, telithromycin was active against actinomyces israelii, actinomyces odontolyticus, bacteroides tectum, bacteroides ureolyticus, bacteroides gracilis (now campylobacter gracilis), porphyromonas spp. (including porphyromonas gingivalis and porphyromonas macacae), prevotella intermedia, prevotella heparinolytica, and almost al ... | 1999 | 10543769 |
| identification of oral actinomyces species using dna probes. | oral actinomyces comprise a major segment of both the supra- and subgingival microbiota; however, little is known about the distribution of individual species in different sites or clinical conditions. the purpose of the present investigation was to develop dna probes for suggested species and genotypes of oral actinomyces. whole genomic dna probes to 12 human oral species and/or serotypes were labeled with digoxigenin and used to seek cross-reactions among the taxa using the checkerboard dna-dn ... | 1999 | 10551171 |
| the effect of scaling and root planing on the clinical and microbiological parameters of periodontal diseases: 12-month results. | previously, we reported that srp resulted in a decrease in mean pocket depth and attachment level and reduced prevalence and levels of bacteroidesforsythus, porphyromonas gingivalis, and treponema denticola at 3 and 6 months post-srp in 57 subjects with adult periodontitis. 32 of the 57 subjects were monitored at 9 and 12 months. thus, the purpose of the present investigation was to evaluate the microbial and clinical effects of srp in 32 (mean age 48+/-11) subjects over a 12-month period. | 2000 | 10674959 |
| actinomycotic ulcer of the oral mucosa: an unusual presentation of oral actinomycosis. | although the oral mucosa is often the site of entry of actinomyces into the deeper tissues, actinomycosis in the oral mucosa is extremely rare. actinomycotic lesions are usually described as either single or multiple abscesses or indurated masses with hard fibrous walls and soft central loculations. actinomyces israelii is the principal cause of human actinomycosis. we present a rare case of actinomycosis caused by actinomyces odontolyticus; it presented primarily as a long-standing ulcer of the ... | 2000 | 10864706 |
| early microbial colonization of permucosal implants in edentulous patients. | in edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ... | 1987 | 10872401 |
| actinomyces canis sp. nov., isolated from dogs. | three strains of a previously undescribed catalase-positive actinomyces-like bacterium were isolated from dogs. biochemical testing and page analysis of whole-cell proteins indicated that the strains were phenotypically highly related to each other but different from previously described actinomyces and arcanobacterium species. sequencing of 16s rrna showed that the unknown bacterium represents a new subline within a cluster of species which includes actinomyces hyovaginalis, actinomyces georgia ... | 2000 | 10939662 |
| microbial composition of supra- and subgingival plaque in subjects with adult periodontitis. | the purpose of the present study was to compare and relate the microbial composition of supra and subgingival plaque in 23 adult periodontitis subjects (mean age 51 +/- 14 years). | 2000 | 11034118 |
| red light kills bacteria via photodynamic action. | with the increase in the number of antibiotic resistant strains of microorganism, the search for alternative treatments of microbial infections becomes all the more important. we report a novel method for bacterial inactivation based on the optical excitation of the naturally occurring (endogenous) photosensitzing porphyrins by red light. in particular, the pathogenic gram-positive porphyrin producing atcc strains propionibacterium acnes, actinomyces odontolyticus and porphyromonas gingivalis we ... | 2000 | 11075959 |
| microbial analysis of bone collected during implant surgery: a clinical and laboratory study. | dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. however, this debris is potentially contaminated with oral bacteria. therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. twenty-four partially dentate patients were randomly al ... | 2001 | 11251657 |
| actinomyces suimastitidis sp. nov., isolated from pig mastitis. | an unusual actinomyces-like bacterium originating from a pig with mastitis was subjected to a polyphasic taxonomic investigation. the morphological and biochemical characteristics of the organism were consistent with its preliminary assignment to the genus actinomyces but it did not appear to correspond to any recognized species. page analysis of whole-cell proteins confirmed the phenotypic distinctiveness of the bacterium and 16s rrna gene sequence analysis demonstrated that it represents a hit ... | 2001 | 11491328 |
| the predominant microflora of nursing caries lesions. | the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ... | 2001 | 11799279 |
| effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions. | the aim of this study was to evaluate the fate of microorganisms in root canals of teeth with infected pulps and periapical bone lesions with and without the use of calcium hydroxide medication. | 2002 | 11858203 |
| germicide effect of several glass ionomer cements. | one the most significant characteristics of glass ionomer cements is their ability to release fluoride compounds. this study was carried out to try establish relationships between this property and the possible effect on the growth of microorganisms that are found in carious lesions, agar bhi medium containing petri dishes were flooded with strains of actinomyces naeslundii, actinomyces israelii and actinomyces odontolyticus. cavities were then prepared in the agar and filled with mixtures of se ... | 1996 | 11885259 |
| the effects of an immediately pre-surgical chlorhexidine oral rinse on the bacterial contaminants of bone debris collected during dental implant surgery. | dental implant surgery produces bone debris that can be used in the "simultaneous augmentation" technique. although this debris is contaminated with oral bacteria, a stringent aspiration protocol has been shown to reduce the levels of contamination. chlorhexidine mouthrinse is a well-proven antibacterial rinse that has been shown to reduce infectious complications associated with dental implants. this study examined the effect of pre-operative rinsing with a 0.1% chlorhexidine digluconate mouthr ... | 2002 | 12005141 |
| [canaliculitis caused by actinomyces in a case of dry eye with punctal plug occlusion]. | we report a case of canaliculitis caused by actinomyces odontolyticus in a case of dry eye with punctal plugs. | 2002 | 12187825 |
| combinations of bacterial species in endodontic infections. | this study was undertaken to investigate combinations of bacteria found in root-canal infections of teeth with periapical bone destruction without clinical signs and symptoms. | 2002 | 12196223 |
| genotypic diversity of clinical actinomyces species: phenotype, source, and disease correlation among genospecies. | we determined the frequency distribution of actinomyces spp. recovered in a routine clinical laboratory and investigated the clinical significance of accurate identification to the species level. we identified 92 clinical strains of actinomyces, including 13 strains in the related arcanobacterium-actinobaculum taxon, by 16s rrna gene sequence analysis and recorded their biotypes, sources, and disease associations. the clinical isolates clustered into 21 genogroups. twelve genogroups (74 strains) ... | 2002 | 12202591 |
| antimicrobial properties of self-etching primer-bonding systems. | self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ... | 2003 | 12670069 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| antibacterial activity of medicinal plant extracts against periodontopathic bacteria. | this study was performed to evaluate the antibacterial activity of althaea officinalis l. roots, arnica montana l. flowers, calendula officinalis l. flowers, hamamelis virginiana l. leaves, illicium verum hook. fruits and melissa officinalis l. leaves, against anaerobic and facultative aerobic periodontal bacteria: porphyromonas gingivalis, prevotella spp., fusobacterium nucleatum, capnocytophaga gingivalis, veilonella parvula, eikenella corrodens, peptostreptococcus micros and actinomyces odont ... | 2003 | 12820224 |
| distribution of selected bacterial species on intraoral surfaces. | to examine the proportions of 40 bacterial species in samples from 8 oral soft tissue surfaces and saliva in systemically healthy adult subjects and to compare these microbiotas with those of supra- and subgingival plaque. | 2003 | 12834503 |
| calibrated latex microspheres percolation: a possible route to model endodontic bacterial leakage. | endodontic therapy is conducted to effectively seal the root canal system. in vitro methods are used to estimate the quality of the seal, generally by measuring any microleakage that allows the tracers to penetrate along the obturated root canal. the use of bacteria as tracers seems to give the most clinically relevant demonstration of microleakage associated with a root canal system. many bacterial strains have been used to evaluate marginal leakage, but results are sometimes contradictory, pro ... | 2003 | 12877263 |
| oral actinomyces isolates forming red colonies on brain heart blood agar can bee unambiguously classified as a odontolyticus by macroscopic examination. | the accurate classification of oral actinomyces isolates as one species is difficult. out of 18 actinomyces isolates forming red colonies on brain heart blood agar, 12 could be straightforwardly assigned as actinomyces odontolyticus by biochemical, morphological, and chemotaxonomic characteristics. for the remaining six isolates, the results of the different identification methods were inconsistent. by sequencing a 16s ribosomal dna fragment by a rapid mass spectrometric method, all isolates cou ... | 2003 | 12904382 |
| lung abscess caused by actinomyces odontolyticus. | a 64-year-old woman with periodontal disease was admitted because of fever, right chest pain, and bloody sputum. her chest radiograph revealed a nodular shadow in the right middle lung fields. from the results of a needle aspiration biopsy, she was diagnosed with a lung abscess due to actinomyces odontolyticus (a. odontolyticus) and streptococcus spp. the patient improved quickly with antibiotics. to our knowledge, only nine cases of pulmonary infection due to a. odontolyticus have been reported ... | 2003 | 12924500 |
| actinomyces spp. in supragingival plaque of ethnic chinese preschool children with and without active dental caries. | very limited molecular epidemiological data are available on the role of actinomyces spp. in the pathogenesis of caries in the primary dentition. therefore, we investigated their distribution in supragingival plaque of ethnic chinese preschool children from singapore and hong kong, either with or without active caries. plaque samples were taken from intact interproximal enamel areas using dental floss. bacterial genomic dna of each sample was extracted and variable regions of 16s ribosomal dna a ... | 2003 | 12925831 |
| the antimicrobial efficacy of 'mgp' gutta-percha in vitro. | to determine whether 'mgp' gutta-percha (westport, ct, usa), a commercially available gutta-percha containing iodoform, inhibits the growth of potential endodontic pathogens. | 2003 | 12950576 |
| actinomyces odontolyticus, a new species of actinomycete regularly isolated from deep carious dentine. | | 1958 | 13576328 |
| frozen cancellous bone allografts: positive cultures of implanted grafts in posterior fusions of the spine. | we have carried out a study on the behaviour pattern of implanted allografts initially stored in perfect conditions (aseptically processed, culture-negative and stored at -80 degrees c) but which presented positive cultures at the implantation stage. there is no information available on how to deal with this type of situation, so our aim was to set guidelines on the course of action which would be required in such a case. this was a retrospective study of 112 patients who underwent a spinal arth ... | 2004 | 14648307 |
| soft-tissue abscess involving actinomyces odontolyticus and two prevotella species in an intravenous drug abuser. | skin and soft-tissue infections in intravenous users comprise a variety of microorganisms and anaerobic bacteria are frequently involved in these suppurative infections. a case of subcutaneous abscess into anterior femoral muscles involving actinomyces odontolyticus and two prevotella species (prevotella buccae and prevotella melaninogenica) in an intravenous drug abuser is presented. this combination of microorganisms has not previously been described in soft-tissue infections. the patient volu ... | 2004 | 14690717 |
| actinomyces odontolyticus bacteremia. | we describe two immunosuppressed female patients with fever and actinomyces odontolyticus bacteremia, a combination documented once previously in an immunocompetent male patient. the patients were treated with doxycycline and clindamycin; these drugs, with beta-lactams, are effective treatment for a. odontolyticus infections. | 2003 | 14720410 |
| the effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored proroot mineral trioxide aggregate. | the purpose of this in vitro study was to determine whether the substitution of 0.12% chlorhexidine gluconate for sterile water as a mixing agent would enhance the antimicrobial activity of tooth-colored proroot mineral trioxide aggregate (mta) against actinomyces odontolyticus (atcc17982), fusobacterium nucleatum (atcc2586), streptococcus sanguis (atcc10556), enterococcus faecalis (er3/2s), escherichia coli (sm10lambdapir), staphylococcus aureus (atcc6538), pseudomonas aeruginosa (ume), and can ... | 2004 | 15167473 |
| exit-site infection caused by actinomyces odontolyticus in a capd patient. | | 2004 | 15559493 |
| evaluation of bacterial nitrate reduction in the human oral cavity. | it is hypothesized that the enterosalivary nitrate circulation encourages nitrate reducing bacteria to reside within the oral cavity. nitrite production may then limit the growth of acidogenic bacteria as a result of the production of antimicrobial oxides of nitrogen, including nitric oxide. this study was carried out with 10 subjects to characterize oral nitrate reduction and identify the bacteria responsible. nitrate reduction varied between individuals (mean 85.4 +/- 15.9 nmol nitrite min(-1) ... | 2005 | 15693824 |
| microarrays complement culture methods for identification of bacteria in endodontic infections. | the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ... | 2005 | 15943771 |
| [a case of pulmonary actinomycosis, who expectorated sulfur granules, caused by actinomyces odontolyticus and actinomyces meyeri]. | a 51-year-old man complaining of cough and bloody sputum, was admitted to our hospital because of antibiotic-resistant chronic pneumonia in the right upper lobe. initially, bronchoscopic examination and sputum culture revealed no evidence of malignancy or any specific infection, either pathologically or microbiologically. however, pathological examination of a solid body expectorated with sputum revealed typical sulfur granules, indicating pulmonary actinomycosis. two actinomyceses named actinom ... | 2005 | 15966370 |
| [disseminated actinomycosis]. | we report the case of a patient suffering from disseminated actinomycosis. | 2005 | 16227933 |