| phylogenetic analysis of alloiococcus otitis gen. nov., sp. nov., an organism from human middle ear fluid. | the partial 16s rrna sequence of an unknown bacterium that was originally isolated from middle ear fluids of children with persistent otitis media was determined by reverse transcription. a comparison of this sequence with sequences from other gram-positive species having low guanine-plus-cytosine contents revealed that this bacterium represents a new line of descent, for which the name alloiococcus otitis gen. nov., sp. nov., is proposed. the type strain is strain ncfb 2890. | 1992 | 1371065 |
| development of a polymerase chain reaction-probe test for identification of alloiococcus otitis. | a rapid polymerase chain reaction test was developed for specific identification of the human middle ear pathogen alloiococcus otitis. primers for the enzymatic amplification reaction were designed from highly specific sequences within the 16s rrna gene. in addition, a confirmatory test based on hybridization of the polymerase chain reaction products to a specific internal probe was developed. | 1992 | 1380011 |
| pcr primers and probes for the 16s rrna gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. | a set of broad-range pcr primers for the 16s rrna gene in bacteria were tested, along with three series of oligonucleotide probes to detect the pcr product. the first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a bacteroides-flavobacterium probe, and two probes for other gram-negative species. the second series was designed to detect pcr products from seven major bacterial species or groups frequently causing meningitis: neisseria mening ... | 1994 | 7512093 |
| atmospheric growth requirements for alloiococcus species and related gram-positive cocci. | the growth of alloiococcus otitis under different atmospheres and nutritional conditions was studied. the growth rates of 25 strains of gram-positive cocci representing five genera on heart infusion agar plates containing 5% rabbit blood and on brucella agar plates with and without sheep blood under aerobic, increased co2, and anaerobic atmospheres were compared. eight strains of alloiococci plated on heart infusion agar with rabbit blood and on brucella sheep blood agar grew under aerobic and c ... | 1996 | 8815077 |
| characterization of ear fluid isolates of alloiococcus otitidis from patients with recurrent otitis media. | nineteen isolates of alloiococcus otitidis from ear fluid samples collected by tympanostomy from patients at four geographic locations were identified by phenotypic characterization and genetic relatedness. initial growth of a. otitidis isolates occurred after 3 days at 37 degrees c on brain heart infusion (bhi) agar with 5% rabbit blood. heavy growth occurred in bhi broth supplemented with 0.07% lecithin and 0.5% tween 80 after 4 days of incubation. the isolates were gram-positive cocci that di ... | 1995 | 8576338 |
| [alloiococcus otitidis: a more common microorganism than we thought?]. | | 1997 | 9235069 |
| identification of bacterial species associated with the sheep scab mite (psoroptes ovis) by using amplified genes coding for 16s rrna. | this was the first molecular study of the bacterial flora of the sheep scab mite (psoroptes ovis). a sequence analysis of genes coding for 16s rrna revealed that serratia marcescens and bacteria closely related to staphylococcus intermedius or staphylococcus chromogens and alloiococcus otitidis were present. these bacteria were associated with skin lesions, dermatitis, and otitis media caused by p. ovis. | 1999 | 10473440 |
| isolation of alloiococcus otitidis from the external ear in children. | | 1999 | 10192719 |
| clinically applicable multiplex pcr for four middle ear pathogens. | the multiplex pcr method for the detection of alloiococcus otitidis, haemophilus influenzae, moraxella catarrhalis, and streptococcus pneumoniae (p. h. hendolin, a. markkanen, j. ylikoski, and j. j. wahlfors, j. clin. microbiol. 35:2854-2858, 1997) in middle ear effusions (mees) was modified to be better suited for clinical use. to detect false-negative results, an internal amplification was added to the reaction, and to prevent carryover contamination, the dutp-uracil-n-glycosidase system was i ... | 2000 | 10618075 |
| detection of alloiococcus otitis in mixed bacterial populations from middle-ear effusions of patients with otitis media. | otitis media is a potentially serious disorder, since there is a risk of permanent hearing loss. culture methods are not useful in characterisation of populations of bacteria in the middle ear. we have used a pcr-based method that does not depend on prior knowledge of the bacteria identified by culture. | 1999 | 10437868 |
| use of multiplex pcr for simultaneous detection of four bacterial species in middle ear effusions. | a multiplex pcr procedure was developed for the simultaneous detection of alloiococcus otitidis, haemophilus influenzae, moraxella catarrhalis, and streptococcus pneumoniae in middle ear effusions (mees) from patients with chronic otitis media with effusion. the bacterial 16s rrna gene was chosen as the target, and the procedure used one common lower primer and four species-specific upper primers. the reaction was optimized by changing the primer concentrations to yield equal amounts of amplific ... | 1997 | 9350746 |
| high incidence of alloiococcus otitis in otitis media with effusion. | the etiology of otitis media with effusion (ome) is unclear. although the majority of effusions show inflammation, culture methods yield positive results for bacteria in only 20 to 30% of cases. | 1999 | 10530580 |
| comparison of three commercial rapid identification systems for the unusual gram-positive cocci dolosigranulum pigrum, ignavigranum ruoffiae, and facklamia species. | we evaluated three rapid identification systems-the biomerieux rapid id 32 strep (id32), the bbl crystal rapid gram-positive identification (crystal), and the remel ids rapid str (ids) systems-for their ability to identify 7 strains of alloiococcus otitidis, 27 strains of dolosigranulum pigrum, 3 strains of ignavigranum ruoffiae, and 18 strains of 4 different facklamia species. since none of these six species of gram-positive cocci are included in the identification databases for these systems, ... | 2000 | 10834950 |
| stimulation of adenoidal lymphocytes by alloiococcus otitidis. | otitis media with effusion (ome) is characterized by persistent effusion in the middle ear cavity and by chronic inflammation in the middle ear mucosa. alloiococcus otitidis, a gram-positive aerobic bacterium, has been isolated in middle ear effusion, and by means of sensitive polymerase chain reaction detection assays it has been detected in as many as 20% of middle ear aspirates of patients with ome. because a otitidis may freely interact with leukocytes in the middle ear effusion, it may pote ... | 2000 | 11051437 |
| otitis media with effusion: the role of alloiococcus otitidis. | otitis media with effusion (ome) is a common condition that can cause deafness as well as speech and behavioural disturbance. it has long been considered to be a noninfective process resulting from eustachian tube dysfunction; however, molecular biological techniques have implicated bacteria in the aetiology of this condition. one such organism is alloiococcus otitidis, which has been detected in middle ear fluid of patients with ome which has not yielded micro-organisms with conventional microb ... | 2001 | 11310934 |
| interleukin-8 secretion of human epithelial and monocytic cell lines induced by middle ear pathogens. | otitis media with effusion (ome) is one of the most common diseases in children. alloiococcus otitidis, a new gram-positive bacterial species, was isolated from the middle ear fluid of children with ome; however, the pathogenic role of this bacteria is yet unknown. in this study, the ability of cultured epithelial cell lines (hep-2 and hela) and monocytic cell lines (thp-1 and u 937) to secrete chemokine interleukin-8 (il-8) in response to the a. otitidis organism and three bacterial organisms m ... | 2000 | 10941934 |
| effect of alloiococcus otitidis and three pathogens of otitis media in production of interleukin-12 by human monocyte cell line. | alloiococcus otitidis is detected in middle ear effusion of otitis media with effusion (ome). only a limited number of studies are available concerning the immunological profile of a. otitidis. we have studied the ability of a. otitidis and three other representative pathogens of otitis media to stimulate the production of interleukin-12 (il-12) from a monocytic cell line thp-1. viable a. otitidis induced the production of il-12 in thp-1 cells but il-12 production was reduced if glutaraldehyde-f ... | 2000 | 11024348 |
| culture-independent molecular analysis of microbial constituents of the healthy human outer ear. | molecular-phylogenetic sequence analyses have provided a new perspective on microbial communities by allowing the detection and identification of constituent microorganisms in the absence of cultivation. in this study we used broad-specificity amplification of ribosomal dna (rdna) genes to survey organisms present in the human outer ear canal. samples were obtained from 24 individuals, including members of three extended families, in order to survey the resident microbiota and to examine microbi ... | 2003 | 12517864 |
| bacterial etiology of otitis media with effusion; focusing on the high positivity of alloiococcus otitidis. | the etiology of otitis media with effusion (ome) is unclear. the bacterial analyses of middle ear effusion (mee) in ome may reveal important information regarding its etiology. alloiococcus otitidis, heamophilus influenzae, streptococcus pneumoniae and moraxella catarrhalis were investigated by using microbiologic culture and a multiplex pcr method in the middle ear fluid of 32 children (54 samples) with chronic ome. pcr yielded positive results in 18 (33.3%) middle ear effusions while culture r ... | 2002 | 11837388 |
| detection of alloiococcus otitidis in the nasopharynx and in the outer ear canal. | alloiococcus otitidis has been recovered from the middle ear of children with otitis media with effusion, but its natural habitat is not known. to determine whether the nasopharynx and the outer ear canals are the natural habitats of a. otitidis, 145 swabs (50, nasopharynx; 95 outer ear canal) collected from 50 children were screened by polymerase chain reaction. a. otitidis dna was detected in seven (4.8%) of the 145 specimens, of which four were nasopharynx, and three outer ear canal. these re ... | 2002 | 12019737 |
| the clinical role of alloiococcus otitidis in otitis media with effusion. | to investigate the presence of alloiococcus otitidis (a. otitidis) in mees from patients with otitis media with effusion (ome) using pcr and to correlate the findings with the clinical picture of children with ome for assessing the clinical role of a. otitidis in ome. | 2002 | 12363421 |
| allofustis seminis gen. nov., sp. nov., a novel gram-positive, catalase-negative, rod-shaped bacterium from pig semen. | an unknown gram-positive, catalase-negative, facultatively anaerobic, non-spore-forming, rod-shaped bacterium originating from semen of a pig was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. chemical studies revealed the presence of a directly cross-linked cell wall murein based on l-lysine and a dna g + c content of 39 mol%. comparative 16s rrna gene sequencing showed that the unidentified rod-shaped organism formed a hitherto unknown subline related, a ... | 2003 | 12807205 |
| bacteriology of chronic maxillary sinusitis and normal maxillary sinuses: using culture and multiplex polymerase chain reaction. | although many investigations have been performed on bacteriology of chronic sinusitis and normal sinuses, there still is much discussion. also a new bacterial agent, alloiococcus otitidis determined in the nasopharynx and middle ear specimens can be thought as a causative agent of sinusitis. | 2003 | 12862402 |
| induction of cd69 expression and th1 cytokines release from human peripheral blood lymphocytes after in vitro stimulation with alloiococcus otitidis and three middle ear pathogens. | alloiococcus otitidis is a recently discovered pathogen of otitis media. however, only a limited number of studies are available about the pathogenic and immunological role of a. otitidis. the aim of this study was to investigate the activation and the cytokine production of human peripheral blood lymphocytes at the early immune response after stimulation with a. otitidis. after stimulation of whole human peripheral blood lymphocytes for 18 h with whole killed a. otitidis or the three major midd ... | 2005 | 15708312 |
| high incidence of alloiococcus otitidis in children with otitis media, despite treatment with antibiotics. | acute otitis media (aom) and otitis media with effusion (ome) are common diseases in childhood. alloiococcus otitidis is a newly recognized species of gram-positive bacterium which was recently discovered as a pathogen associated with ome. although some studies show that a. otitidis is frequently detected in children with ome, no study is available concerning the clinical efficiency of antibiotics against this organism. the prevalence of a. otitidis in 116 middle ear effusion specimens from 36 a ... | 2006 | 16517881 |
| alloiococcus otitidis in acute otitis media. | the bacterium alloiococcus otitidis has been found to be associated with otitis media with effusion (ome). when the culture method is used, its detection rate is low, whereas applying the polymerase chain reaction (pcr) yields significantly higher frequencies. this study was carried out to investigate the incidence of a. otitidis in children with acute otitis media (aom). | 2004 | 14687687 |
| high frequency of alloiococcus otitidis in the nasopharynx and in the middle ear cavity of otitis-prone children. | colonization of middle ear pathogens is facilitated in the nasopharynx of otitis-prone children, and is associated with the development of otitis media. recently, a new species of bacterium, alloiococcus otitidis, is considered as one of the major middle ear pathogens. however, as far as we know, no study has been reported concerning the prevalence of a. otitidis in the nasopharynx of otitis-prone children. and, no study has been conducted on the association of a. otitidis in the nasopharynx wit ... | 2006 | 16310863 |
| atopococcus tabaci gen. nov., sp. nov., a novel gram-positive, catalase-negative, coccus-shaped bacterium isolated from tobacco. | a novel gram-positive, aerobic, catalase-negative, coccus-shaped organism originating from tobacco was characterized using phenotypic and molecular taxonomic methods. the organism contained a cell wall murein based on l-lysine (variation a4alpha, type l-lysine-l-glutamic acid), synthesized long-chain cellular fatty acids of the straight-chain saturated and monounsaturated types (with c(16:1)omega9, c(16:0) and c(18:1)omega9 predominating) and possessed a dna g+c content of 46 mol%. based on morp ... | 2005 | 16014503 |
| revised nomenclature of alloiococcus otitis. | | 1993 | 16789267 |
| alloiococcus otitidis is a ligand for collectins and toll-like receptor 2, and its phagocytosis is enhanced by collectins. | alloiococcus otitidis has been found to be associated with otitis media with effusion. in this study we investigated whether tlr2 and collectins, surfactant protein a (sp-a) and mannose-binding lectin (mbl), interacted with a. otitidis. both sp-a and mbl bound to a. otitidis in a ca(2+)-dependent manner. a. otitidis induced il-8 secretion from u937 cells and nf-kappab activation in tlr2-transfected hek293 cells. however, the cells transfected with the mutant tlr2(p681h) did not respond to a. oti ... | 2006 | 16708401 |
| evidence of local antibody response against alloiococcus otitidis in the middle ear cavity of children with otitis media. | alloiococcus otitidis is a recently discovered bacterium frequently associated with otitis media. however, no study is available as to whether a. otitidis has a pathogenic role and induces local immune response in the middle ear as a true pathogen. whole bacterial sonicate of a. otitidis was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), and transferred to a nitrocellulose membrane. then, western blot analysis was performed with supernatant of the middle ear e ... | 2007 | 17094788 |
| interleukin-8 induction via nf-kappab, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 pathways in human peripheral blood mononuclear cells by alloiococcus otitidis. | alloiococcus otitidis is a newly recognized species of gram-positive bacteria frequently associated with otitis media. although immunostimulating activity of this organism has been investigated, little is known about the signaling pathways of chemokine/cytokine induction by this organism. we investigated the role of nf-kappab and mitogen-activated protein (map) kinases in chemokine interleukin-8 (il-8) production by human peripheral blood mononuclear cells (pbmcs) after stimulation with a. otiti ... | 2007 | 17629956 |
| isolation of alloiococcus otitidis from indigenous and non-indigenous australian children with chronic otitis media with effusion. | during the last decade alloiococcus otitidis has been identified in specimens from patients with chronic otitis media with effusion. whereas most of those studies employed molecular techniques, we used minor modifications of conventional microbiological methods to isolate and identify a. otitidis in samples obtained from 20/50 (40%) children referred for myringotomy. alloiococcus otitidis was isolated from 10/22 (45%) indigenous and 10/28 (36%) non-indigenous children. this is the first report o ... | 2007 | 17666076 |
| isolation and characterization of dibenzofuran-degrading serratia marcescens from alkalophilic bacterial consortium of the chemostat. | alkalophilic bacterial consortium developed by continuous enrichment in the chemostat in presence of 4-chlorosalicylic acid as sole source of carbon and energy contained six bacterial strains, micrococcus luteus (csa101), deinococcus radiothilus (csa102), csa103 (burkholderia gladioli), alloiococcus otilis (csa104), micrococcus diversus (csa105), micrococcus luteus (csa106), identified by the biolog test method. the strains were tested for utilization of organic compounds in which one of the str ... | 2007 | 17710482 |
| [bacterial implication in otitis media with effusion in the childhood]. | our main aim is to analyze the bacterial involvement in otitis media with effusion (ome) and its effect on antimicrobial therapy. | 2007 | 17999905 |
| a polysaccharide of alloiococcus otitidis, a new pathogen of otitis media: chemical structure and synthesis of a neoglycoconjugate thereof. | alloiococcus otitidis is a recently discovered gram-positive bacterium that has been linked with otitis media (middle ear infections). in this study, we describe the structure of a novel capsular polysaccharide (ps) expressed by the type-strain of a. otitidis, atcc 51267, and the synthesis of a glycoconjugate composed of the capsule ps and bovine serum albumin (bsa). the capsule ps of a. otitidis type-strain was determined to be a repeating trisaccharide composed of 3-substituted n-acetyl-d-gluc ... | 2008 | 18336800 |
| preliminary study of proinflammatory cytokines and chemokines in the middle ear of acute otitis media due to alloiococcus otitidis. | alloiococcus otitidis is a newly discovered organism frequently detected in otitis media. however, the association of the organism with the development of otitis media has not been disclosed in detail yet. in the middle ear, proinflammatory cytokines and chemokines are released in association with infection by pathogens, and these cytokines contribute to the induction of an inflammatory reaction. to investigate the profile of inflammation-related cytokines in the acute phase of a. otitidis infec ... | 2009 | 19185927 |
| serous otitis media in children: implication of alloiococcus otitidis. | otitis media with effusion (ome) has long been considered to be a noninfective disease resulting from a eustachian tube dysfunction. however, several microbiological techniques have shown bacteria in the middle ear fluids from patients with ome. alloiococcus otitidis has been detected in the middle ear fluid from patients with ome. the exact role of this infectious agent in the pathogenesis of ome has yet to be elucidated. | 2008 | 18418283 |
| alloiococcus otitidis--otitis media pathogen or normal bacterial flora? | during the last decade a new potential otitis media pathogen, alloiococcus otitidis, has been studied. it is still not clear whether this bacterium really is a pathogen, although it has been found in a high percentage of middle ear effusions in children. the present study aimed to investigate the presence of a. otitidis in the nasopharynx and outer ear canals, and to develop a culture method that would make it possible to isolate a. otitidis from these locations. nasopharyngeal samples (n = 129) ... | 2008 | 19024598 |
| otitis media microbes: culture, pcr, and confocal laser scanning microscopy. | to assess the presence of middle ear pathogens in nasopharynx (np), middle ear fluid (mef), and middle ear mucosal swabs (mes) of 14 patients undergoing middle ear surgery. | 2009 | 19670592 |
| lack of chlamydophila pneumoniae and predominance of alloiococcus otitidis in middle ear fluids of children with otitis media with effusion. | to investigate the presence of chlamydophila pneumoniae and other bacterial pathogens in middle ear effusion samples obtained from children with otitis media with effusion (ome). | 2010 | 19879704 |
| the prevalence of middle ear pathogens in the outer ear canal and the nasopharyngeal cavity of healthy young adults. | culturing middle ear fluid samples from children with chronic otitis media with effusion (ome) using standard techniques results in the isolation of bacterial species in approximately 30-50% of the cases. haemophilus influenzae, streptococcus pneumoniae and moraxella catarrhalis, the classic middle ear pathogens of acute otitis media, are involved but, recently, several studies suggested alloiococcus otitidis as an additional pathogen. in the present study, we used species-specific pcrs to estab ... | 2010 | 19895585 |
| simultaneous assay for four bacterial species including alloiococcus otitidis using multiplex-pcr in children with culture negative acute otitis media. | the 3 most commonly encountered bacteria in acute otitis media (aom) are streptococcus pneumoniae, haemophilus influenzae, and moraxella catarrhalis. conventional culture methods detect these pathogens in only 60% to 70% of cases of aom. alloiococcus otitidis, another potential pathogen, has often been ignored. | 2010 | 20335823 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| impact of anaerobic and oligotrophic conditions on survival of alloiococcus otitidis, implicated as a cause of otitis media. | the survival of alloiococcus otitidis (ncfb2890) with different nutritional supplements, including brain-heart infusion broth (bhi), phosphate-buffered saline (pbs), distilled water (dw), and middle ear effusion (mee), as well as various atmospheres (aerobic, microaerobic, anaerobic), was compared using cultures, live/dead staining, and transmission electron microscopy. the bacterial morphological traits and viability were maintained in bhi and mee under aerobic conditions but were rapidly lost ... | 2011 | 21203796 |
| spontaneously draining acute otitis media in children: an observational study of clinical findings, microbiology and clinical course. | abstract objectives: to study the outcome of acute otitis media (aom) with otorrhoea in children managed initially without antibiotics, in relation to bacterial and clinical findings, and to identify those who may benefit from antibiotics. methods: otherwise healthy, not otitis prone children aged 2-16 y, presenting with aom with spontaneous otorrhoea, were recruited from primary care and followed at selected ear, nose and throat (ent) clinics. specimens for bacterial investigations were obtaine ... | 2011 | 21736512 |
| Nasopharyngeal carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Alloiococcus otitidis in young children in the era of pneumococcal immunization, Taiwan. | We applied a multiplex polymerase chain reaction (PCR) and culture to detect Streptococcus pneumoniae and detected 3 other respiratory pathogens--Haemophilus influenzae, Moraxella catarrhalis, and Alloiococcus otitidis--simultaneously by PCR, in the nasopharynx of 386 children aged under 5 y. S. pneumoniae was the most common pathogen carried by children in all age groups, with the rate ranging from 15.8% in children aged 3-4 y to 28.6% in children aged 2-3 y. H. influenzae and M. catarrhalis sh ... | 2011 | 21892897 |
| alloiococcus otitidis: an emerging pathogen in otitis media. | | 2011 | 22120114 |