| [on the serology and immunbiology of listeriosis. vii. communication: further investigations on the agglutination-immobilization test (author's transl)]. | factor antisera oriented to different serovars of listeria moncytogenes, expecially to serovar 7 and murray grayi (listeria grayi) were tested. it was found that the agglutination-immobilization test detects antibodies oriented to the h-antigen. by contrast, the growth inhibition test measures antibodies oriented to the o-antigen. summarizingly, it can be stated that the following three seroreactions, each based on a different mode of action, are available for the determination of antibodies spe ... | 1979 | 95075 |
| a numerical taxonomic survey of listeria and related bacteria. | a numerical taxonomic study was performed on named strains of listeria, erysipelothrix, microbacterium thermosphactum, lactobacillus, streptococus, propionibacterium, kurthia and some possibly related bacteria using i43 unit characters covering a wide range of properties. the strains fell into six main clusters: (a) listeria; (b) microbacterium thermosphactum, lactobacillus, streptococcus; (c) gemella, erysipelothrix; (d) kurthia and mainly aerobic corynebacteria; (e) propionibacterium, staphylo ... | 1977 | 856940 |
| screening for listeria monocytogenes and other related species from faecal specimens at the lagos university teaching hospital, lagos. | four hundred and twenty faecal specimens from patients with acute gastroenteritis and apparently healthy persons who reported at the lagos university teaching hospital (luth) between october, 1988 and may, 1989 were investigated for faecal carriage of listeria monocytogenes and other related species. of these specimens, none was positive for listeria species. however, the mannitol--fermenting listeria species now murraya grayi sub species grayi and murraya grayi sub spp. murrayi representing 0.9 ... | 1992 | 1308081 |
| structural and functional properties of the p60 proteins from different listeria species. | the major extracellular protein p60 of listeria monocytogenes seems to be required for this microorganism's adherence to and invasion of 3t6 mouse fibroblasts but not for adherence to human epithelial caco-2 cells. western blot analysis with polyclonal antibodies against p60 of l. monocytogenes indicated the presence of cross-reacting proteins in the culture supernatants of all listeria species. protein p60 of l. monocytogenes could restore adhesion of the l. monocytogenes mutant riii (impaired ... | 1992 | 1459966 |
| phosphatidylinositol-specific phospholipase c activity as a marker to distinguish between pathogenic and nonpathogenic listeria species. | in this study, 468 listeria strains were checked for the presence of phosphatidylinositol-specific phospholipase c (pi-plc) activity by using a simple assay that consisted of overlaying colonies formed on agar plates with l-alpha-phosphatidylinositol as substrate. in this assay, pi-plc-active colonies show turbid halos around the colonies as a result of the release of insoluble diacylglycerol from the substrate. this activity was detected only in the pathogenic species listeria monocytogenes and ... | 1991 | 1662937 |
| o antigenic structure of listeria grayi and listeria murrayi. | cross-agglutination and absorption studies indicated that two serological reference strains of listeria grayi and listeria murrayi differed in at least one o factor. the serological analysis revealed that the unshared o factors are not present in other listeria serovars. these results are not in accordance with the antigenic structure previously reported for l. grayi and l. murrayi, then a new o antigen formulation is proposed for these species: l. grayi (iii), xii, xiv and xvi; l. murrayi (iii) ... | 1990 | 1702256 |
| phylogenetic analysis of the genus listeria based on reverse transcriptase sequencing of 16s rrna. | the phylogenetic interrelationships of members of the genus listeria were investigated by using reverse transcriptase sequencing of 16s rrna. the sequence data indicate that at the intrageneric level the genus listeria consists of the following two closely related but distinct lines of descent: (i) the listeria monocytogenes group of species (including listeria innocua, listeria ivanovii, listeria seeligeri, and listeria welshimeri) and (ii) the species listeria grayi and listeria murrayi. at th ... | 1991 | 1713054 |
| assignment of listeria grayi and listeria murrayi to a single species, listeria grayi, with a revised description of listeria grayi. | the genomic relatedness between listeria grayi and listeria murrayi was reevaluated by using dna-dna hybridization, multilocus enzyme electrophoresis, and rrna restriction fragment length polymorphism techniques. the high levels of similarity observed between the strains of these two species confirmed the data published since 1973 and indicated that they should be considered members of a single species. on grounds of priority, the species should be named l. grayi. | 1992 | 1736964 |
| evaluation of an enzymatic method for fast identification of listeria spp. in cheese and meat products. | listeria-tek elisa was compared with conventional cultural procedure of fil/idf (federation international de laiterie/international dairy federation) modified by us. the elisa assay is able to detect listeria innocua, listeria monocytogenes, listeria murrayi and listeria welshimeri, after 48 h in artificially contaminated cheeses. no specific reactions, no false positive or negative were detected and the assay has a high correlation with the conventional method used in this research. we also ana ... | 1991 | 1750966 |
| sensitive and specific detection of listeria monocytogenes in milk and ground beef with the polymerase chain reaction. | a sensitive and specific method for detection of listeria monocytogenes in milk and ground-beef samples is described. it consists of culturing samples in listeria enrichment broth (leb) and subculturing them from leb to listeria plating media, followed by dna extraction and species-specific detection of the organism by using the polymerase chain reaction (pcr). in developing the l. monocytogenes pcr assay, five oligonucleotide primers complementary to the nucleotide sequence of the listeriolysin ... | 1991 | 1768130 |
| taxonomy of the genus listeria by using multilocus enzyme electrophoresis. | seventy-three strains of the seven recognized listeria species were studied by performing a multilocus enzyme electrophoresis analysis of 18 enzyme loci. the mean number of alleles per locus was 9.5 and all of the loci were polymorphic. a total of 56 electrophoretic types were distinguished. cluster analysis of a matrix of the genetic distances between paired electrophoretic types revealed that there were six principal clusters at the species level (genetic distances between clusters greater tha ... | 1991 | 1899799 |
| haematological reactions of rabbits infected intravenously with listeria strains of different virulence. | listeria strains of different virulence were injected intravenously into rabbits of both sexes (2-4 kg). the infectious dose was 10(8) cells/kg. blood samples were taken from the ear wein one day and immediately before the infection, then 3 h, 1, 2, 3, 6 and 10 days after it. total white blood cell counts and their changes were determined and the number of lysosomal granules in neutrophils were counted. a marked monocytic reaction was observed after the injection of virulent listeria monocytogen ... | 1990 | 2124767 |
| the place of listeria among gram-positive bacteria. | the genus listeria, containing the species listeria monocytogenes, listeria innocua, listeria seeligeri, listeria welshimeri, listeria ivanovii, listeria grayi and listeria murrayi is a well circumscribed taxon. numerical taxonomic and chemical studies indicate a very close phenetic similarity to the genus brochothrix and a more distant, but close, similarity to a number of other genera such as lactobacillus, streptococcus, lactococcus, enterococcus, staphylococcus, kurthia and bacillus. recent ... | 1988 | 2458321 |
| taxonomy of the genus listeria. | according to dna homology values as well as to 16s rrna cataloguing results, the genus listeria presently contains two groups of closely related species: one is constitued by listeria gravi and listeria murrayi, the other by listeria monocytogenes, listeria ivanovii, listeria innocua, listeria welshimeri, and listeria seeligeri. among these seven species, only two are pathogenic for humans and animals: listeria monocytogenes and listeria ivanovii. listeria denitrificans was recently excluded fro ... | 1988 | 2458322 |
| serological studies on listeria grayi and listeria murrayi. | a cross-agglutination study between somatic antigens from reference strains of listeria grayi and listeria murrayi with rabbit antisera was done. l. murrayi antisera reacted, at low titres, with l. grayi but l. grayi antisera did not react with l. murrayi antigen. these results, together with agglutinin-absorption tests, led to the conclusion that the serologic relationship between l. grayi and l. murrayi is not as close as is thought. the two species seem to differ in at least one somatic facto ... | 1988 | 2459098 |
| physiological studies on the growth and utilization of sugars by listeria species. | experiments, relevant to growth in milk, were done to delineate the aerobic and anaerobic growth of listeria species on selected sugars in several media. all species grew on glucose aerobically, forming lactic acid and (or) acetic acid. anaerobically, only lactic acid was formed; cell yields were 80% of those obtained aerobically. when incubated aerobically, small amounts (1.5 microns/ml) of isovaleric acid, 2-hydroxyisovaleric acid, and trace amounts of isobutyric acid were formed. these produc ... | 1989 | 2501014 |
| specific gene probe for detection of biotyped and serotyped listeria strains. | a total of 284 strains of listeria, including all known serovars and biovars together with listeria grayi and listeria murrayi, were biotyped and serotyped. biotyping and serotyping could be done in 2 days. a gene probe encoding a delayed hypersensitivity factor (dth) was used in the detection of pathogenic biotypes and serotypes of the tested strains. the gene was found in all 117 tested listeria monocytogenes strains of serogroups 1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4c, 4d, 4e, 4ab, and 7. it was al ... | 1989 | 2658807 |
| [listeria monocytogenes in pasteurized milk]. | an haemolytic listeria monocytogenes strain pathogenic to mice was isolated from 6 out of 28 (21.4%) pasteurized milk samples (3.2% fat milk treated at 78 degrees c for 15 s) marketed by a madrid processing plant. listeria grayi was recovered from 25 of the samples (89.2%) and l. innocua from 3 samples (10.7%). one milk sample was contaminated with l. welshimeri. no strains of l. ivanovii, l. seeligeri, l. murrayi, or l. denitrificans were isolated. these results show that pathogenic listeria st ... | 1986 | 3084052 |
| potential use of continuous cell lines to distinguish between pathogenic and nonpathogenic listeria spp. | continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic listeria species. listeria monocytogenes and listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while listeria innocua, listeria grayi, and listeria murrayi strains were negative in all three tests. the eight cell lines tested were all affected but varied in sensitivity, with the chinese hamster ovary ... | 1987 | 3114320 |
| hemolysin from listeria--biochemistry, genetics and function in pathogenesis. | thiol-activated hemolysins (listeriolysins) from listeria monocytogenes (sv4b) and listeria ivanovii were purified to homogeneity. the n-terminal amino acid sequences of the 58 kda listeriolysin of l. ivanovii and of a 24 kda protein which may represent the camp-factor of l. ivanovii were determined. antibodies raised against the l. ivanovii listeriolysin and anti-streptolysin o antibodies were used in western blot analyses to detect listeriolysin(s) in virulent and avirulent listeria strains. i ... | 1988 | 3138189 |
| comparison of seven plating media for enumeration of listeria spp. | the suitability of seven media for the enumeration of listeria spp. was evaluated at 30 degrees c for 48 h. the media tested were (i) the original mcbride listeria agar formulation (with glycine); (ii) modified mcbride agar containing glycine anhydride; (iii) licl-phenylethanol-moxalactam (lpm) agar; (iv) acriflavine-ceftazidime agar; (v) rodriguez isolation agar (risa); (vi) modified vogel-johnson (mvj) agar; (vii) cyclohexanedione-nalidixic acid-phenylethanol agar; and tryptose agar as control ... | 1988 | 3146947 |
| [isolation of micro-organisms of the species listeria from raw milk intended for human consumption]. | refrigerated mixtures of raw milk provided by a dairy which was supplied by farms from west and central spain were tested for the presence of listeria microorganisms. a total of 95 samples were taken at regular intervals over a 16-month period. listeria grayi was isolated from 89.5% of the samples, listeria monocytogenes s. str. from 45.3%, listeria innocua from 15.8%, listeria welshimeri from 3.1%, and listeria seeligeri from 1.05%. listeria ivanovii, listeria murrayi, and listeria denitrifican ... | 1985 | 4063881 |
| specific antibody-mediated detection of brochothrix thermosphacta in situ in british fresh sausage. | a rabbit polyclonal antibody-linked probe was developed which detected 76% of 800 food isolates of the spoilage bacterium brochothrix thermosphacta when cells were bound to nitrocellulose. in slide cross-reaction tests all six environmental isolates tested were stained but the type strain was not. the antibody did not cross-react with listeria grayi, l. monocytogenes, lactobacillus plantarum, lactococcus lactis, streptococcus mutans, bacillus cereus or b. subtilis. the antibody-linked probe dete ... | 1995 | 7538105 |
| study of the prevalence of listeria spp. in surface water. | the quantity of listeria species was determined in 49 samples of various types of water (river, brackish water, urban wastewater). twenty nine strains of listeria were isolated. these were mainly identified as listeria monocytogenes (72,4%), but listeria innocua, listeria grayi, listeria ivanohovii and listeria welshimeri were also present. with the exception of water taken from the estuary, 74.4% of samples contained listeria in mean concentrations of between 2 mpn/100 ml and 1320 mpn/100 ml in ... | 1994 | 7848499 |
| discrimination of species in the genus listeria by fourier transform infrared spectroscopy and canonical variate analysis. | infrared spectra of type cultures of the six recognized species of the genus listeria and of listeria grayi subsp. murrayi were recorded. by use of a library of 59 spectra, comprising at least six replicates of each type, discrimination by canonical variate analysis of the spectral amplitudes allowed all of the spectra to be correctly classified. | 1995 | 7887620 |
| expression of superoxide dismutase in listeria monocytogenes. | the nature and expression of superoxide dismutase (sod; ec 1.15.1.1) in the gram-positive food-borne pathogen listeria monocytogenes were examined. metal depletion and reconstitution studies and resistance to h2o2 and potassium cyanide inactivation indicated that l. monocytogenes has a single sod which utilizes manganese as a metal cofactor. the specific activity of sod was unchanged in cells exposed to a heat shock at 42 degrees c or grown in the presence of paraquat-generated superoxide anion ... | 1994 | 8074517 |
| comparative analysis of 16s and 23s rrna sequences of listeria species. | in order to establish the taxonomic value of 16s rrna and 23s rrna for distinguishing listeria species, the complete 23s rrna sequences for all listeria species were determined by using the type strains. we designed and experimentally validated a universal 23s rrna sequencing method, which included pcr amplification of the rdna gene and direct cycle sequencing of the amplicon with eubacterial primers. the results of our sequence comparison indicated that the genus listeria can be divided into tw ... | 1996 | 8782674 |
| genus- and species-specific detection of listeria monocytogenes using polymerase chain reaction assays targeting the 16s/23s intergenic spacer region of the rrna operon. | in this study, the 16s/23s rrna intergenic spacer (igs) regions of six listeria species were examined. dna bands of 590 and 340 bp were observed following polymerase chain reaction (pcr) amplification of dna from listeria monocytogenes, listeria innocua, listeria seeligeri, listeria welshimeri, and listeria ivanovii strains with generic rrna igs oligonucleotide primers. for strains of listeria grayi subspp. grayi and murrayi, dna band sizes of 550 and 340 bp were observed with this primer pair. ... | 1996 | 8941989 |
| unstable expression and thermal instability of a species-specific cell surface epitope associated with a 66-kilodalton antigen recognized by monoclonal antibody em-7g1 within serotypes of listeria monocytogenes grown in nonselective and selective broths. | conditions that resulted in unstable expression and heat instability of a cell surface epitope associated with a 66-kda antigen in listeria monocytogenes serotypes were identified with the probe monoclonal antibody (mab) em-7g1 in an enzyme-linked immunosorbent assay. this epitope appeared to be absent in three serotypes (serotypes 3b, 4a, and 4c), which did not react with mab em-7g1 irrespective of the enrichment broth tested. the remaining 10 serotypes were detected by mab em-7g1 only when cel ... | 1998 | 9687476 |
| incidence of listeria monocytogenes in cheese produced in rio de janeiro, brazil. | the present study evaluated the incidence of listeria spp. in some brazilian cheeses obtained from retail stores in rio de janeiro, of 103 samples of various types of cheese examined as recommended in the listeria isolation protocol of the health protection branch of canada, 11 (10.68%) were contaminated by listeria monocytogenes, 13 (12.62%) by listeria innocua, 6 (5.83%) by listeria grayi, and 1 (0.97%) by listeria welshimeri. a higher incidence of l. monocytogenes as observed mainly in the ho ... | 1998 | 9708310 |
| occurrence of listeria monocytogenes in sewage sludge. | the search for salmonella is the only bacteriological index included in italian guidelines (in accordance with eu regulations) for the use of sewage sludge in agriculture. as a result, information regarding the presence of listeria monocytogenes is rather limited. we therefore decided to carry out an investigation of listeria in the sludge produced by the bologna (italy) treatment plant during the various phases of treatment. five different types of sludge were analysed (primary raw, activated, ... | 1998 | 9789361 |
| listeria species in raw and ready-to-eat foods from restaurants. | from september 1999 to march 2000, meat (pork, beef, and chicken), fish (salmon, hake, and sole), vegetable (lettuce and spinach), and spanish potato omelette samples obtained at restaurants were collected and tested for the occurrence of listeria spp. listeria monocytogenes was isolated from 3 (2.9%) out of 103 studied samples. other species isolated were listeria grayi (13.6%), listeria innocua (1.9%), listeria ivanovii (5.8%), listeria seeligeri (3.9%), and listeria welshimeri (1.9%). listeri ... | 2001 | 11307896 |
| mode of action, purification and amino acid sequence of plantaricin c19, an anti-listeria bacteriocin produced by lactobacillus plantarum c19. | plantaricin c19, an anti-listeria bacteriocin, was successfully purified by adsorption to and release from producing cells at low ph combined with reverse phase high-performance liquid chromatography (hplc). the purification resulted in a 900-fold increase in specific activity with a yield of 15% of the original activity. mass spectrometry analysis gave a molecular weight of 3845.3. protein microsequencing identified 36 amino acids. plantaricin c19 is rich in both hydrophobic and basic amino aci ... | 2001 | 11545225 |
| occurrence of listeria spp. in critical control points and the environment of minas frescal cheese processing. | critical control points (ccps) associated with minas frescal cheese (a brazilian soft white cheese, eaten fresh) processing in two dairy factories were determined using flow diagrams and microbiological tests for detection of listeria monocytogenes and other species of listeria. a total of 218 samples were collected along the production line and environment. the ccps identified were reception of raw milk, pasteurization, coagulation and storage. thirteen samples were positive for listeria; 9 sam ... | 2003 | 12485750 |
| pcr detection of bacillus and staphylococcus in various foods. | a broad-range pcr assay for the detection of bacteria belonging to bacillus and staphylococcus genera was developed. primers targeting the bacterial 16s rrna gene were newly designed and used in a pcr assay. to determine the specificity of the assay, 81 different bacterial strains (of 50 genera), 2 fungi, 3 animals, and 4 plants were tested. results were positive for every tested bacillus, staphylococcus, or aerococcus strain. in addition, the result for listeria grayi was positive with lower pc ... | 2004 | 15222564 |
| evolutionary history of the genus listeria and its virulence genes. | the genus listeria contains the two pathogenic species listeria monocytogenes and listeria ivanovii and the four apparently apathogenic species listeria innocua, listeria seeligeri, listeria welshimeri, and listeria grayi. pathogenicity of the former two species is enabled by an approximately 9 kb virulence gene cluster which is also present in a modified form in l. seeligeri. for all listeria species, the sequence of the virulence gene cluster locus and its flanking regions was either determine ... | 2005 | 15709360 |
| isolation and pcr amplification of a species-specific oxidoreductase-coding gene region in listeria grayi. | listeria grayi is a nonpathogenic gram-positive bacterium that demonstrates considerable similarities to other members in the genus listeria, including the foodborne human pathogen listeria monocytogenes and the animal pathogen listeria ivanovii. a rapid diagnostic test to identify and diagnose listeriosis would be valuable, especially in cases where the presence of l. grayi may complicate diagnosis. this test would be based on a unique gene present in l. grayi. in this study, after comparative ... | 2005 | 15782240 |
| effect of ph on the inhibition of listeria spp. by vanillin and vanillic acid. | the antimicrobial effects of vanillin and vanillic acid were verified against several species and strains of listeria monocytogenes, listeria innocua, listeria grayi, and listeria seeligeri in a laboratory medium adjusted to ph values ranging from 5.0 to 8.0. medium ph had little influence on the mic of vanillin as determined by a broth dilution assay, and growth of all test strains was inhibited by concentrations ranging from 23 to 33 mm. in contrast, none of the strains were inhibited by 100 m ... | 2005 | 16013390 |
| does sporadic listeria gastroenteritis exist? a 2-year population-based survey in nova scotia, canada. | febrile gastroenteritis due to listeria monocytogenes (lm) has been primarily described in foodborne outbreaks. we decided to determine the incidence of sporadic, febrile gastroenteritis due to lm in a large, well-defined north american population over a 2-year period and to compare these cases to sporadic cases of campylobacter and salmonella infections occurring concurrently in the community. | 2005 | 16107973 |
| monoclonal antibodies binding to the cell surface of listeria monocytogenes serotype 4b. | serotype 4b strains of the food-borne pathogen listeria monocytogenes are responsible for a large portion of sporadic listeric infections and all major food-borne listeriosis outbreaks in humans. hybridomas were produced from three fusions with lymphocytes of nd4 mice immunized either with the insoluble antigens of l. monocytogenes serotype 4b or with formalin-killed bacterial cells and screened for monoclonal antibodies (mabs) reactive to l. monocytogenes serotype 4b. a set of 35 mabs was ident ... | 2006 | 16476793 |
| real-time pcr detection of 16s rrna genes speeds most-probable-number enumeration of foodborne listeria monocytogenes. | quantifying foodborne pathogens at concentrations of 0.1 to 1,000 cfu/g of food generally involves most-probable-number (mpn) enumeration, which takes at least 4 days. a real-time pcr assay (rti-pcr) was developed to accelerate mpn enumeration of foodborne listeria monocytogenes. foods were spiked from 70 to 110 cfu/g, and triplicate subportions from 0.0001 to 1 g were selectively enriched for 48 h at 30 degrees c. for standard mpn enumeration, the enrichments were subcultured on oxford agar (48 ... | 2007 | 17685338 |
| listeria grayi bacteremia in a heart transplant recipient. | bacteria belonging to the genus listeria have been isolated from food products of animal, plant, and fish origin, and are associated with infections in immunocompromised hosts, pregnant women, and infants. the species listeria grayi has rarely been reported as a human pathogen. it has a unique antibiotic sensitivity profile. we describe a case of l. grayi bacteremia in a heart transplant recipient. the organism demonstrated a reduced sensitivity to ampicillin. the patient was successfully treate ... | 2008 | 18811634 |
| prevalence of listeria, aeromonas, and vibrio species in fish used for human consumption in turkey. | a total of 78 raw retail fish samples from 30 freshwater and 48 marine fish were examined for the presence of listeria, aeromonas, and vibrio species. the overall incidence of listeria spp. was 30% in freshwater samples and 10.4% in marine fish samples. listeria monocytogenes (44.5%) was the most commonly isolated species in freshwater fish, and listeria murrayi (83.5%) was the most commonly isolated species in marine fish samples. motile aeromonads were more common in marine fish samples (93.7% ... | 2010 | 20132688 |
| prevalence and contamination patterns of listeria monocytogenes in catfish processing environment and fresh fillets. | catfish skins, intestines, fresh fillets, processing surfaces at different production stages, chiller water and non-food contact surfaces were sampled for listeria monocytogenes and other listeria species. among 315 samples, prevalence of l. monocytogenes, listeria innocua and a group of listeria seeligeri-listeria welshimeri-listeria ivanovii was 21.6, 13.0 and 29.5%, respectively. no listeria grayi was detected in this survey. while no l. monocytogenes strains were isolated from catfish skins ... | 2010 | 20510783 |
| listeria grayi: vancomycin-resistant, gram-positive rod causing bacteremia in a stem cell transplant recipient. | we report the first case of listeria grayi bacteremia in a stem cell transplant recipient. the patient developed bacteremia with a gram-positive rod that was initially thought to be corynebacterium species and a skin contaminant. the organism grew in multiple blood cultures and therapy with vancomycin was initiated. the api coryne (version 3.0) identified the organism as l. grayi. susceptibility testing by etest suggested that the organism was resistant to vancomycin, but susceptible to ampicill ... | 2010 | 20626713 |
| microbiological quality of grey-mullet roe. | the greek grey-mullet roe is produced from the fully developed gonads of the female mullet (mugil cephalus) couth in lagoons during their reproductive migration. the traditional processing method of the roe includes, air drying, salting, shape formation and covering with multiple layers of natural beeswax for preservation and distribution. fish roe brands have been a staple in local diet and is increasingly becoming popular in the international market. as a ready-to-eat food it's microbial quali ... | 2011 | 21497664 |
| in vitro anti-listerial activities of crude n-hexane and aqueous extracts of garcinia kola (heckel) seeds. | we assessed the anti-listerial activities of crude n-hexane and aqueous extracts of garcinia kola seeds against a panel of 42 listeria isolates previously isolated from wastewater effluents in the eastern cape province of south africa and belonging to listeria monocytogenes, listeria grayi and listeria ivanovii species. the n-hexane fraction was active against 45% of the test bacteria with zones of inhibition ranging between 8-17 mm, while the aqueous fraction was active against 29% with zones o ... | 2011 | 22072929 |
| control of listeria monocytogenes growth in a ready-to-eat poultry product using a bacteriophage. | a bacteriophage (phage) that infected strains of the species listeria monocytogenes as well as listeria ivanovii and listeria welshimeri, but not listeria grayi or listeria innocua, was isolated from sheep faeces. the phage had a contractile tail and an icosohedral head indicating that it was a myovirus, and was morphologically similar to phage a511. at 30 °c, phages added at 5.2 × 10⁷ pfu ml⁻¹ prevented the growth in broth of l. monocytogenes present at approximately twice this concentration fo ... | 2011 | 21925027 |
| defensins from the tick ixodes scapularis are effective against phytopathogenic fungi and the human bacterial pathogen listeria grayi. | ixodes scapularis is the most common tick species in north america and a vector of important pathogens that cause diseases in humans and animals including lyme disease, anaplasmosis and babesiosis. tick defensins have been identified as a new source of antimicrobial agents with putative medical applications due to their wide-ranging antimicrobial activities. two multigene families of defensins were previously reported in i. scapularis. the objective of the present study was to characterise the p ... | 2014 | 25443032 |
| cytotoxic effect and tlc bioautography-guided approach to detect health properties of amazonian hedyosmum sprucei essential oil. | bioautography has been used as rapid and easy strategy to detect and identify bioactive fractions/molecules in the never before investigated hedyosmum sprucei solms (chloranthaceae) essential oil (eo). the antioxidant activity, performed through dpph bioautographic assay and spectrophotometric evaluations (ic50 = 230 ± 10 µg/ml), seemed to be mainly due to α-cadinol and α-muurolol. (hp)tlc bioautography, focused on antimicrobial capacities, pointed out α-cadinol, α-muurolol, τ-muurolol, caryophy ... | 2016 | 27118979 |
| influence of cutting and deboning operations on the microbiological quality and shelf life of buffalo meat. | considering the specific biochemical composition of buffalo (bubalus bubalis) meat (high iron content, high biological value proteins and essential fatty acids, low amounts of fat and cholesterol), we evaluated the influence of cutting and deboning operations on the microbiological quality and shelf-life of vacuum-packed buffalo meat stored under refrigeration. on the processing day, samples were collected from carcass, deboning room surfaces and meat cuts. samples from meat cuts were evaluated ... | 2016 | 26897086 |
| evaluation of the anti-listeria potentials of some plant-derived triterpenes. | listeriosis is a fatal disease caused by pathogenic listeria bacteria and it is most prevalent in immune-compromised individuals. the increase in numbers of immune-compromised individuals against a background of listeria antibiotic resistance, limits listeriosis treatment options. this therefore calls for research into substitute treatments, of which, medicinal plants derived compounds offer a viable alternative. | 2014 | 25056181 |
| listeria monocytogenes: an emerging food-borne pathogen and its public health implications. | listeria monocytogenes is considered one of the most important food-borne pathogens transmitted to humans via contaminated food. the aim of the present study was to demonstrate the importance of l. monocytogenes as a food-borne pathogen. | 2016 | 26927456 |
| polymerase chain reaction detection of listeria monocytogenes on frankfurters using oligonucleotide primers targeting the genes encoding internalin ab. | a polymerase chain reaction (pcr) assay targeting the genes encoding internalin ab (inlab) was developed for detecting listeria monocytogenes in pure cell cultures and on artificially contaminated frankfurters. four sets of oligonucleotide primers were evaluated. the set targeting a 902-bp region of the inlab gene was the most specific. this pcr product was detected in 51 l. monocytogenes strains belonging to four different serogroups (1/2a, 1/2b, 1/2c, and 4b). in contrast, the pcr product was ... | 2003 | 12597483 |
| prevalence and contamination levels of listeria monocytogenes in smoked fish and pâté sold in spain. | from march to november 2000, 170 samples of smoked fish and 182 samples of pâté for sale in retail outlets and supermarkets in the nine provinces of castilla and león (spain) were analyzed for the prevalence of listeria monocytogenes and other listeria spp. l. monocytogenes was isolated from 38 (22.3%) of the 170 samples of smoked fish analyzed. twenty of these positive samples contained l. monocytogenes at >100 cfu/g. other listeria spp., such as listeria innocua (26 isolates), listeria grayi ( ... | 2001 | 11770642 |
| bacteriophage typing of listeria species. | a bacteriophage typing scheme for differentiating listeria isolates from dairy products and various other foodstuffs was developed. sixteen selected phages isolated from both environmental sources and lysogenic strains were used for typing and, according to their lytic spectra, divided into four groups. thus far, 41 distinct patterns of lysis were seen when this set was used in typing 57 defined reference strains, representing all five confirmed species and 16 serotypes in addition to 454 lister ... | 1990 | 2116763 |
| purification of a surface-specific soluble antigen from listeria monocytogenes. | a complex antigenic preparation obtained from listeria monocytogenes serovariant 4b by freeze-pressing, centrifugation, and gel filtration treatment was studied by crossed immunoelectrophoresis, with the aim of preparing an antigenic fraction that could be used to investigate the serological response to listeric infection. of 17 immunoprecipitates revealed in the soluble extract, one of three major antigens (designated antigen 2) was shown to be a strong antigen in humans or rabbits infected wit ... | 1979 | 115793 |
| the murein types of listeria grayi, listeria murrayi and listeria denitrificans. | the amino acid sequences of the mureins of the type strains listeria grayi atcc 19120, listeria murrayi atcc 25401 and listeria denitrificans atcc 14870 were determined. l. grayi and l. murrayi contain the murein variation a1γ (a(2)pm-direct-type) which is also present in the type strain of l. monocytogenes. l. denitrificans has a different murein. it belongs to the variation a4α, exhibiting a γ-d-glutamyl-l-seryl-interpeptide bridge between alanyl-γ-d-glutamyl-lysyl-d-alanine peptide subunits ( ... | 1983 | 23194802 |
| prevalence and characterization of listeria species in domestic and industrial cheeses of isfahan region. | listeria monocytogenes is of major concern to the food industry in general and the dairy industry in particular. little is known about incidence of this pathogenic bacterium in dairy products in iran. | 2011 | 23113091 |
| listeria weihenstephanensis sp. nov., isolated from the water plant lemna trisulca taken from a freshwater pond. | the phylogenetic position and phenotypic characteristics of two non-spore-forming bacilli similar to members of the genus listeria were studied. the gram-reaction-positive, slightly motile, facultatively anaerobic strains were isolated from the water plant lemna trisulca sampled from a freshwater pond in bavaria, germany. although no identification was possible employing the api listeria test (biomérieux), 16s rrna sequence analysis confirmed a close phylogenetic similarity to listeria rocourtia ... | 2013 | 22544790 |
| development and validation of qualitative sybr®green real-time pcr for detection and discrimination of listeria spp. and listeria monocytogenes. | a combination of four qualitative sybr®green qpcr screening assays targeting two levels of discrimination: listeria genus (except listeria grayi) and listeria monocytogenes, is presented. these assays have been developed to be run simultaneously using the same polymerase chain reaction (pcr) programme. the paper also proposes a new validation procedure to specifically validate qpcr assays applied to food microbiology according to two guidelines: the iso 22118 norm and the "definition of minimum ... | 2013 | 23086339 |
| listeria floridensis sp. nov., listeria aquatica sp. nov., listeria cornellensis sp. nov., listeria riparia sp. nov. and listeria grandensis sp. nov., from agricultural and natural environments. | sampling of agricultural and natural environments in two us states (colorado and florida) yielded 18 listeria-like isolates that could not be assigned to previously described species using traditional methods. using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average blast nucleotide identity (anib) of less than 85% to currently described species. phylogenetic analysis based on 16s rrna gene sequences and amino acid sequen ... | 2014 | 24599893 |
| comparative genomics of listeria sensu lato: genus-wide differences in evolutionary dynamics and the progressive gain of complex, potentially pathogenicity-related traits through lateral gene transfer. | historically, genome-wide and molecular characterization of the genus listeria has concentrated on the important human pathogen listeria monocytogenes and a small number of closely related species, together termed listeria sensu strictu. more recently, a number of genome sequences for more basal, and nonpathogenic, members of the listeria genus have become available, facilitating a wider perspective on the evolution of pathogenicity and genome level evolutionary dynamics within the entire genus ... | 2015 | 26185097 |
| characteristics and distribution of listeria spp., including listeria species newly described since 2009. | the genus listeria is currently comprised of 17 species, including 9 listeria species newly described since 2009. genomic and phenotypic data clearly define a distinct group of six species (listeria sensu strictu) that share common phenotypic characteristics (e.g., ability to grow at low temperature, flagellar motility); this group includes the pathogen listeria monocytogenes. the other 11 species (listeria sensu lato) represent three distinct monophyletic groups, which may warrant recognition a ... | 2016 | 27129530 |
| prevalence and antimicrobial resistance of listeria species isolated from different types of raw meat in iran. | listeria and particularly listeria monocytogenes are important foodborne pathogens that can cause listeriosis and severe complications in immunocompromised individuals, children, pregnant women, and the elderly. the objective of this study was to determine the prevalence of listeria spp. in raw meat in iran. from july 2010 to november 2011, a total of 1,107 samples of various raw meats were obtained from randomly selected retail butcher shops. the results of conventional bacteriologic and pcr me ... | 2012 | 23212021 |
| identification of six listeria species by real-time pcr assay. | the listeria genus comprises 10 recognized species. listeria monocytogenes causes listeriosis in humans and other animals primarily via contaminated food or animal feed. listeria ivanovii causes listeriosis in animals and on rare occasions in humans. the identification of nonpathogenic species of listeria in foods indicates that conditions exist that support the growth of pathogenic strains and is used to facilitate the implementation of control and prevention measures. this study shows the deve ... | 2014 | 24461044 |
| biophysical principles predict fitness landscapes of drug resistance. | fitness landscapes of drug resistance constitute powerful tools to elucidate mutational pathways of antibiotic escape. here, we developed a predictive biophysics-based fitness landscape of trimethoprim (tmp) resistance for escherichia coli dihydrofolate reductase (dhfr). we investigated the activity, binding, folding stability, and intracellular abundance for a complete set of combinatorial dhfr mutants made out of three key resistance mutations and extended this analysis to dhfr originated from ... | 2016 | 26929328 |
| incidence and persistence of listeria monocytogenes in the catfish processing environment and fresh fillets. | incidence of listeria spp. in whole raw catfish, catfish fillets, and processing environments from two catfish processing facilities was determined in august 2008 and august 2009. thirty-nine (18.4%) of 212 samples collected in august 2008 were positive for listeria monocytogenes. prevalences of listeria species l. innocua and l. seeligeri-l. welshimeri-l. ivanovii were 11.3 and 23.6%, respectively. of 209 samples collected in august 2009, 12.4% were positive for l. monocytogenes, 11% for l. inn ... | 2010 | 20828470 |
| genotypic characterization of listeria spp. isolated from fresh water fish. | a total of 200 samples (muscles and viscera, 100 of each) of fresh water fish, walking catfish (clarias batrachus) were screened for listeria spp. all the samples were subjected to a two-step enrichment followed by plating on selective media. confirmation of the isolates was on the basis of biochemical characters, haemolysis on blood agar and christie, atkins, munch petersen test. a total of 39 isolates of listeria spp. were recovered. of these 26 (67%), 8 (21%), 3 (8%) and 2 (5%) were listeria ... | 2007 | 17182144 |