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detection of experimental bacteremia and fungemia by examination of buffy coat prepared by a micromethod.rabbits received intravenous injections of bacteria or fungi, and a comparison was made of the abilities of broth cultures, plating after dilution either in saline solution or in distilled water containing triton x-100, and buffy coat examinations to detect the organisms in heart blood. the most sensitive method was broth culture. by microscopy or subculture of buffy coat cells prepared by centrifugation of blood in microhematocrit tubes, organisms were rapidly and regularly detected when their ...197938662
non-specific antimicrobial cellular resistance in sensitized guinea pigs.specific delayed-type hypersensitivity was induced in guinea pigs with bovine albumin + complete freund adjuvant, bovine gamma globulin + complete freund adjuvant and bcg vaccine. the animals were subsequently tested for nonspecific antimicrobial resistance. sensitized and control groups were challenged intraperitoneally with listeria monocytogenes 2 hr after reinjection with the sensitizing antigen. the listeria content of the spleens was determined 1 or 5 days after the infection. the number o ...197659519
generation of macrophage chemotactic activity in situ in listeria-immune rats. 197771948
hospital outbreak of listeria monocytogenes septicaemia: a problem of cross infection?a 74-year-old man was admitted to hospital with septicaemia and meningitis caused by listeria monocytogenes. subsequently 3 inpatients developed listeria septicaemia. case-to-case transmission of the infection seems possible, although the method of transmission remains obscure.197882046
assessment of blood leukocyte microbial killing by using a new fluorochrome microassay. 197990723
practical considerations in using counterimmunoelectrophoresis to identify the principal causative agents of bacterial meningitis.many clinical laboratories are currently using counterimmunoelectrophoresis (cie) as an aid in the rapid diagnosis of bacterial meningitis. because cross-reactions among causative agents have been reported, the present study was undertaken to explore the problems that might occur when reference and commercial antisera are used in cie. broth cultures of 35 bacterial strains were tested with 76 reference and commercial antisera by cie. some of the antisera tested failed to react with their homolog ...197993606
ia antigens in serum during different murine infections.there exists in the mouse a family of i-region-controlled (ia) antigens which carry carbohydrate-defined determinants. these antigens appear in serum as glycolipids and seem to be actively secreted by antigen-activated t-cells. this paper describes the ability of selected viral, bacterial, and protozoal infections of mice to markedly alter the serum levels of these ia antigens. all the infectious agents examined induced substantial augmentation or suppression of serum ia concentrations or both. ...197994905
the comparative effects of isoprinosine, levamisole, muramyl dipeptide and sm1213 on lymphocyte and macrophage proliferation and activation in vitro. 197995186
regulation of the immune response by macrophages.regulation of the immune response by macrophages was studied with cellular resistance to listeria monocytogenes as parameter. the use of agents which suppress macrophage activity during the induction-phase of immunity enabled the induction of protective immunity with killed listeria. fractionation of the cell content of listeria yielded an rna'se sensitive fraction which in a dose of 300 ng and in combination with the cationic surfactant dimethyl dioctadecyl ammonium bromide induced protective i ...197995385
increased phagocytic activity of splenectomized mice challenged with listeria monocytogenes.adult splenectomized mice exhibit increased resistance to infection with listeria monocytogenes. phagocytosis, by reticulo-endothelial cells, of test particles (51cr-labelled sheep erythrocytes) is the same in splenectomized and control mice. however, 24 h exposure to listeria, which fails to influence phagocytic activity of normal mice, greatly enhances the blood clearance and liver uptake of the test particles in splenectomized mice. the presence of a cell population and/or product in the sple ...197896011
effects of isoprinosine, levamisole, muramyl dipeptide, and sm1213 on lymphocyte and macrophage function in vitro.four immunopotentiators, levamisole, isoprinosine, muramyl dipeptide, and sm1213, were analyzed in vitro for their effect on phytohemagglutinin-induced lymphocyte proliferation and lymphokine-induced macrophage proliferation and activation to kill listeria monocytogenes. isoprinosine augmented all three functions. levamisole augmented lymphocyte proliferation and lymphokine-induced macrophage activation. muramyl dipeptide and sm1213 induced macrophage activation directly. the effects of these ag ...197883192
allogeneic restriction of the delayed inflammatory reaction in the rat.delayed-type hypersensitivity (dth) to listeria monocytogenes was measured in rats that were recipients of syngeneic, semisyngeneic, and allogeneic immune thoracic duct lymphocytes (tdl). dth could be transferred only to recipients that shared at least one haplotype with the tdl donors. the restriction was expressed in an inability of sensitized lymphoblasts to localize efficiently at antigen injection sites in the pinna of the ear and peritoneal cavity. failure of allogeneic lymphoblasts to ext ...197879605
[characterization of flagella and flagellin of listeria monocytogenes. i. immunological investigations (author's transl)]. 197660251
shared antigens between human malignant melanoma cells and mycobacterium bovis (bcg).this study was undertaken to investigate the antigenic relationships between human malignant melanoma cells and mycobacterium bovis (bcg). rabbits were immunized with sonicates of bcg or with malignant melanoma cells from different patients and the resulting antisera were tested for their capacity to bind radiolabeled soluble extracts prepared from bcg and melanoma cells. the binding of antibodies to radiolabeled antigens was studied by precipitation of radiolabeled antigen-antibody complexes by ...197658033
shared antigens between bacteria and guinea pig line 10 hepatocarcinoma cells.this study was undertaken to investigate the possibility that listeria monocytogenes, brucella abortus, and salmonella typhimurium share antigenic components with guinea pig line 10 hepatocarcinoma cells. rabbits were immunized with sonicates of these bacteria or line 10 tumor cells. other rabbits were immunized with line 1 cells, a tumor with antigenic characteristics different from those of line 10. the binding of antibodies to radiolabeled antigens prepared from extracts of bacteria and line ...197657823
allogeneic restriction of acquired antimicrobial resistance in the rat.allogeneic restriction of acquired antimicrobial resistance was demonstrated by infusing listeria-immune thoracic duct lymphocytes (tdl) into syngeneic, semisyngeneic, or allogeneic recipients that had been infected i.v. with listeria monocytogenes (lm). syngeneic and semisyngeneic recipients enjoyed a high level of protection (3 to 5 log10 reduction in viable organisms within 64 hr, whereas allogeneic recipients were poorly protected (0.5 to 2.5 log10 reduction). sharing one haplotype between t ...197898584
listeria monocytogenes infection in neonates: investigation of an epidemic.from march 10 through october 29, 1975, listeria monocytogenes infection occurred in seven neonates born at a 401-bed general hospital in greenville, s.c. in the preceding 19 months, there had been only one case. six of the seven isolates from the infected infants were of serotype jb. risk of listeriosis in neonates was associated with being born to mothers of low socioeconomic status and being born to mothers who had had vaginitis during pregnancy. nosocomial transmission of l. monocytogenes ap ...197898594
inhibition by gangliosides of hemolysis induced by streptolysin o and listeria monocytogenes hemolysin [proceedings]. 197898658
resistance to tumor growth mediated by listeria monocytogenes. destruction of experimental malignant melanoma by lm-activated peritoneal and lymphoid cells.a murine experimental model of nonspecific tumor destruction mediated by cells activated by listeria monocytogenes (lm) is described. b16 melanoma growth is prevented or suppressed in the syngeneic host when tumor cells are inoculated in contact with viable lm. in vitro, cultured b16 cells are destroyed by lm immune peritoneal or splenic cells in the presence of the bacterial antigen(s). activation of lm immune cells in vitro is immunologically specific. replacement of lm by sheep red blood cell ...197656396
depression of protective mechanisms against microörganisms in tumor-bearing mice and its restoration by adjuvants.bacterial growth within 72 hr after an intravenous inoculation with listeria monocytogenes was enhanced strikingly in the liver of mice, when viable cells of sarcoma-180 were injected subcutaneously into ddn, c3h/he, and balb/c mice 5 hr before the inoculation. such an enhanced bacterial growth appears to be attributable to a depressed ability of macrophages to digest engulfed bacteria. pretreatments with zymosan, killed bcg, or viable bcg prevented such depression in tumor-bearing mice and incr ...197799344
role for activated macrophages in resistance against trichinella spiralis.to determine whether activated macrophages are important in resistance against the intestinal phase of nematode parasites, we studied trichinella spiralis infections in mice with normal macrophages and in mice with macrophages activated by either chronic toxoplasma gondii or acute listeria monocytogenes infections. the peak t. spiralis adult worm burden in the intestines of normal c57bl/6 or swiss webster mice occurred from 6 to 14 days after infection. subsequent expulsion of worms from the int ...197899366
haemophilus somnus complex: antigenicity and specificity of fractions of haemophilus somnus.five haemophilus somnus type 8025 preparations (whole cell, sonicate, crude polysaccharide, purified polysaccharide, and protein) were produced for studies of their antigenicity in rabbits. bacterial agglutination and passive hemagglutination tests were used to assess the level of antibody produced in rabbits inoculated with the different antigenic preparations. cross-reactions were seen between the antiserums against the h sumnus 8025 antigens and a variety of related and unrelated bovine patho ...197550755
rabbit immunoglobulin allotypes: structure, immunology, and genetics. 197550001
microbiology problem. 1978101083
acquired cellular resistance, delayed hypersensitivity, and altered macrophage migration in listeria monocytogenes-infected guinea pigs.a listeria monocytogenes infection in guinea pigs was used to study the interrelationship between antigen-induced macrophage migration inhibition, delayed-type hypersensitivity, and acquired cellular resistance. early after infection (at 2 and 7 days), very significant enhancement of macrophage migration was observed. migration inhibition was detected beginning on day 14 and was uniformly observed only on day 21 of the infection, after which a shift again to enhancement was seen. the early detec ...1978101454
correlative ultrastructural and functional study of hamster peritoneal macrophage activation in vitro by lymphokines. 1978101660
macrophage activation by lymphocyte mediators. 197548483
monocyte separation from whole blood. a method for experimental animals. 197546200
listeriosis in sheep. isolation of listeria monocytogenes from grass silage. 197944661
activation of macrophages assessed by in vivo and in vitro tests. 197944641
correlation between in vivo and in vitro functional tests for activated macrophages.mice undergoing graft-versus-host reaction, skin grafting, and inoculation with tumor cells were tested for nonspecific resistance by intravenous challenge with listeria monocytogenes. peritoneal exudate macrophages from mice treated in a similar manner were tested in vitro for increased degradation of [1-14c]glucose, ability to degrade antigen/antibody complexes, ability to inhibit intracellular growth of listeria, and staining for beta-galactosidase. there was good correlation between in vivo ...197933898
a method for the rapid isolation of listeria monocytogenes from infected material. 197720438
relation of concentrations of bacteria and bacterial antigen in cerebrospinal fluid to prognosis in patients with bacterial meningitis. 197713301
epidemic listeriosis. report of 14 cases detected in 9 months.during the period august 1977 to april 1978, 14 black patients in johannesburg area had systemic infections caused by listeria monocytogenes. nine of these patients were neonates who presented with septicaemia (5 cases) or septicaemia and meningitis (4 cases) and 5 were adults, all of whom had meningitis. the mortality rate was 43% (6/14), with 4 neonatal and 2 adult deaths. all isolates of l. monocytogenes were type 4b. only sporadic cases of human listeriosis have previously been reported in s ...1978104398
[studies about monocines (author's transl)].by means of mitomycin induction monocine preparations were made from 162 listeria monocytogenes strains. from tested strains of different serovars and antigen combinations altogether 76% were monocinogenic. examination of efficiency was performed against 15 indicator strains of listeria monocytogenes. concluding from spectra of reaction 6 groups of monocines (a-f) could be differentiated. serovar 5 (ivanov) proved as an indicator strain particularly sensitive for all monocines.1978104479
clinical aspects on 64 cases of juvenile and adult listeriosis in sweden.in 1958-74 altogether 64 cases of bacteriologically verified infections of listeria monocytogenes were diagnosed in sweden in children, aged more than 27 days, and in adults. immunosuppression predisposed to the disease. thus, many patients had co-existing disorders, such as leukemia and alcoholism. sixteen patients had been treated with corticosteroids, which were combined with cytostatic drugs in nine. meningoencephalitis was diagnosed in 52 patients and was fatal in 16. the clinical symptoms ...1978104552
interaction of listeria monocytogenes and influenza in an animal model.this study was designed to investigate the effects of viruses in the pathogenesis of listeria monocytogenes. the organisms used in this study were: listeria monocytogenes type 1 isolated from a local fatal case; mouse adapted influenza a/pr8/34 (honi); streptococcus pneumoniae group b (u.m. med. ctr.) and poliovirus type 2 mef--g3m2. balb-c mice were inoculated intraperitoneally with one ld50 of listeria monocytogenes. ten days later, the survivors were challenged intransally with 10 ld50 of inf ...197610263
the mediator of cellular immunity. xii. inhibition of activated t cells by newcastle disease virus.newcastle disease virus (ndv) can interact in at least two ways with rat t cells. by adsorbing to circulating lymphocytes, the virus can transiently deflect the cells from lymph nodes and inflammatory exudates induced in the peritoneal cavity. t cells are affected regardless of age, state of activation, or position in the mitotic cycle. the effect is reversible and is mediated not only by infectious (i)-ndv, but also by uv-ndv which cannot achieve a complete replication cycle in eggs. but i-ndv ...19768579
functional properties of t and b cells isolated by affinity chromatography from rat thoracic duct lymph. 19766153
bacterial meningitis in infancy and childhood in lusaka (one year prospective sturdy).in approximately 10,000 admissions in a 12 months period, at university teaching hospital, lusaka 85 cases of meningitis were recorded. the signs and symptoms in these patients do not greatly differ from other similar studies in africa. the commonest organism isolated was pneumococcus. there was high mortality rate which was to a large extent due to parents not bringing their children to hospital early enough for medical treatment to be instituted. this is borne out by the fact that 50% of the c ...19755834
antitumor activity of listeria monocytogenes on a guinea pig fibrosarcoma.listeria monocytogenes-mediated tumor inhibition was studied in strain 13 guinea pigs by using a methylcholanthrene-induced fibrosarcoma (mca-1). mixtures of listeria and tumor cells in ratios of 1:100, 1:200, or 1:400 (listeria:mca-1 cells) led to significant suppression of tumor growth. intralesional injection of tumors on day 6 posttransplantation led to the regression of a highly significant number of tumors. animals receiving injections of listeria, either in a mixture with tumor cells or i ...1979106006
macrophage activation during experimental murine brucellosis: a basis for chronic infection.evidence is presented that the chronicity of infection in cba mice after injection of brucella abortus 19 is related to a number of factors: (i) the relative resistance of b. abortus to macrophage killing, which allowed some bacteria to survive the peak of macrophage activity occurring at 14 days; (ii) the decline in macrophage activity thereafter (this decline was related in part to the presence of fewer bacteria to stimulate the bactericidal activity and also to specific, active suppressor mec ...1979106010
[features of cellular immunity in several bacterial infections]. 197896635
[listeriasis in man and animal in the netherlands (1958-1977)]. 1979107461
[induction of macrophage activation with bacterial products for potentiation of resistance to some chronic infections].adult mice of the consanguine a2g strain received two intraperitoneal inoculations of 0.5 mg listeria monocytogenes phospholipid extract at 30 days interval. in the treated animals and the controls, the capacity to develop a humoral immune response was tested 24 hours after the second inoculation, following antigenic stimulus with sheep red blood cells; phagocytizing in vitro of labelled germs by peritoneal macrophages and the response of lymphocytes to mitogenic and allogenic stimuli were likew ...1978107569
postnatal development of resistance against infection in an experimental model.the postnatal development of resistance against infection was monitored by the treatment of juvenile mice with a virulent strain of listeria monocytogenes. it could be shown that until day 10 after birth, young mice succumbed to an infection with even minimal doses of bacteria. between day 15 and 30, the resistance against infection gradually increases until the rather constant level of grown-up animals is reached (fig. 1). juvenile mice that survive the primary infection are able to build up a ...1978107682
[resistance to infection with listeria monocytogenes in normal and thymusless mice treated with ampicillin (author's transl)].nmri mice were infected intravenously with a sublethal dose of listeria monocytogenes and divided into four groups. one group served as the control and the other three were treated with ampicillin beginning 4, 8 or 24 hours after infection. the animals were injected in the morning and in the evening each time with 4 mg ampicillin subcutaneously until a total dose of 48 mg was reached. as demonstrated by counting of the bacteria in the spleen, listeria could multiply in the ampicillin treated mic ...197896629
intrathecal gentamicin for refractory gram-positive meningitis.the mortality for meningitis due to staphylococcus aureus or listeria monocytogenes remains high, ranging from 32% to 77%. we treated a patient with staphylococcal meningitis refractory to methicillin sodium and oxacillin sodium, and a patient with listeria meningitis refractory to penicillin g potassium and ampicillin sodium; both patients were cured when intrathecal gentamicin sulfate was added to the regimen. the combination of intrathecal gentamicin and a penicillin may be beneficial in refr ...197896281
human listeriosis in peru.in a survey on listeriosis in peru a study was made of 653 stool samples; material from 8 cases of perinatal death, in which a macroscopic diagnosis of listeriosis had been made at autopsy; 1 sample of vaginal secretion from a woman with abortion due to listeriosis; and 1 case of abscess which turned out to be an hematometra. the following results have been obtained: 1. from the faeces 7 isolates of listeria (1.07%) were obtained: 3 belonged to l. monocytogenes serovar 4ab, 3 to l. innocua (the ...1979108825
[gram-positive bacteria as adjuvants (author's transl)].gram-positive bacteria or their constituents influence the immune response in various ways. for instance: listeria monocytogenes cellwall fraction acts as b-cell mitogen, in this way enhancing a humoral immune response. corynebacterium parvum acts as a macrophage stimulant evoking maximum macrophage activation. bcg, among other things, leads to an enhancement of a t-cell response to unrelated antigens. the hypothetical therapeutic consequences are discussed.197896006
effect of listeria monocytogenes lipids on the course of infections with some gram-negative bacilli in mice.the effect of listeria monocytogenes lipids on the course of infections with salmonella enteritidis, yersinia pseudotuberculosis, pseudomonas aeruginosa and klebsiella pneumoniae was studied in mice. the lipids were extracted with chloroform and methanol and used in various doses before and after infection with the bacilli. the lipids significantly increased mouse resistance toward the examined bacteria. the protective effect depended, to a large extent on the applied dose of lipids and its timi ...1979109064
augmented resistance to listeria monocytogenes in mice at an early stage of aging.protective mechanisms against listeria monocytogenes were studied in young (3-month-old) and old (15-month-old) mice of c3h/he strain. cumulative mortality rates of old mice were lower than those of young mice after intravenous inoculation of the same doses of bacteria. the numbers of bacteria in the liver and spleen on days 1 and 3 were larger in young than old mice. bacterial growth at this stage of infection is suppressed by accumulation of non-immune macrophages. on day 7, however, the numbe ...197995807
[on the serology and immunbiology of listeriosis. vii. communication: further investigations on the agglutination-immobilization test (author's transl)].factor antisera oriented to different serovars of listeria moncytogenes, expecially to serovar 7 and murray grayi (listeria grayi) were tested. it was found that the agglutination-immobilization test detects antibodies oriented to the h-antigen. by contrast, the growth inhibition test measures antibodies oriented to the o-antigen. summarizingly, it can be stated that the following three seroreactions, each based on a different mode of action, are available for the determination of antibodies spe ...197995075
antibiotic susceptibility of clinical isolates of listeria monocytogenes.a broth microdilution method was used to measure the minimal inhibitory concentrations (mics) of the antibiotics most often recommended for treatment of listeriosis. the mics of ampicillin, penicillin, erythromycin, and tetracycline for 175 strains of listeria monocytogenes were below the approximate mic breakpoint for susceptible strains as recommended by the national committee on clinical laboratory standards. inhibition diameters for 125 strains were measured by the standardized disk method ( ...197896737
comparison of bacterial cardiotoxins: thermostable direct hemolysin from vibrio parahaemolyticus, streptolysin o and hemolysin from listeria monocytogenes.some properties of the bacterial cardiotoxins, thermostable direct hemolysin from vibrio parahaemolyticus (vibriolysin), and streptolysin o and hemolysin from listeria monocytogenes (listeriolysin), were compared. these toxins had rapid lethal effects on mice when injected intravenously. the electrocardiographic changes of rats after intravenous injections of these toxins were very similar, showing bradycardia and inhibition of atrio-ventricular conduction. these toxins also caused cessation of ...197896814
activated lymphocytes trigger lymphoblast extravasation. 197896942
listeria monocytogenes cell walls induce decreased resistance to infection.a significant decrease in murine resistance to listeria monocytogenes was induced by using crude listeria cell wall fraction (lcwf) and purified listeria cell walls (pf). when equal amounts of these materials were injected, pf was more effective than lcwf in decreasing resistance. the pf effect was dose dependent when measured either as a decrease in 50% lethal dose of the listeria challenge or as a decrease in survival time of the infected mice. pf apparently does not act directly on the lister ...197897226
[listeria monocytogenes meningitis after renal transplantation. report of a case]. 197898821
listerial prosthetic valve endocarditis. successful medical therapy. 1978100621
[meningitis caused by listeria monocytogenes]. 1979111319
[neonatal listeriosis]. 1979111338
[soluble listeria monocytogenes antigen in cerebrospinal fluid]. 1978100765
listeria monocytogenes endophthalmitis with a black hypopyon.a 68-year-old woman had a marked decrease in visual acuity, increased intraocular pressure, and acute iridocyclitis. she developed a pigmented hypopyon simulating an occult intraocular melanoma. two anterior chamber paracenteses showed growth of listeria monocytogenes. the patient received systemic intravenous penicillin, topical fortified gentamicin sulfate drops, and intraocular injections of cephaloridine. on discharge from the hospital after a two-week stay, visual acuity had improved and in ...1978102203
delayed-type hypersensitivity and acquired cellular resistance in mice immunized with killed listeria monocytogenes and adjuvants.delayed-type hypersensitivity (dh) and acquired cellular resistance (arc) to listeria monocytogenes in mice was studied following immunization with killed bacteria in combination with freund's complete adjuvant or the adjuvant dimethyldioctadecylammonium bromide (dda). intracutaneous or intraperitoneal injections of killed listeria mixed with freund's complete adjuvant did neither result in dh nor in acr. intracutaneous injections of killed listeria and dda resulted in an antigen-dose dependent ...1979112043
an attempt at immunotherapy of murine virus erythroleukemia.it was demonstrated that the nonvirulent strain of listeria monocytogenes isolated from vegetable matter prolonged significantly the survival time of mice inoculated with murine virus erythroleukemia. the most significant survival time was recorded when live listeriae were administered on the same day but into another site than the leukemia virus was.1978102689
perinatal and neonatal infections: listeriosis. 1979112093
[meningitis caused by listeria monocytogenes--an autopsy case]. 1978102810
use of a macrophage cytotoxicity system to show macrophage activation by listeria monocytogenes cell wall fraction.experiments were conducted to determine whether a partially purified listeria cell wall fraction could stimulate macrophages to high levels of activation. to detect activation of macrophages, a macrophage-mediated cytotoxicity system were established. the data demonstrate that listeria cell wall components are capable of activating thioglycollate-induced adherent peritoneal exudate cells to be cytotoxic for 51cr-labelled target tumour cells, and that the listeria fraction is as effective as bact ...1979112673
[listeriasis in goats]. 1979112711
light and electron microscopic study of the livers of pregnant mice infected with listeria monocytogenes.listeria monocytogenes cells were observed in the hepatic cell cytoplasm or in the phagosome at 24 and 48 hours but not at 72 hours after inoculation in pregnant mice. the presence of bacteria initially in a membrane-bound vesicle indicates that the bacteria enter the hepatic cells by endocytosis, resulting in eventual destruction of hepatic cells. characteristic lesions of the liver at 24 and 48 hours after inoculation consist of multiple focal areas of necrosis. the initial neutrophilic reacti ...1978103471
immunity to antigenically related salmonellae: effects of humoral factors on the bactericidal activity of normal and immune peritoneal exudate cells.immunity against salmonella enteritidis and listeria monocytogenes was studied by measuring in vitro the bactericidal activity of peritoneal exudate cells (pec) of control (normal pec) and s. typhi ty2-immune (immune pec) mice. specific immune serum, anti-s. tyhphi ty2, heat inactivated at 56 degrees c for 30 min, significantly inhibited the growth of s. enteritidis only with immune pec. these opsonic factors had no effect upon the activity of normal pec. that such inhibition could not be demons ...1978103836
[epidemiology and diagnosis of listeria infections. 1]. 1979113195
heat-labile b-cell mitogen obtained from listeria monocytogenes.a water-soluble extract of listeria monocytogenes strain 10403 acts as a mitogen on cultured mouse spleen lymphocytes. this mitogen induced a response six to nine times that of controls, as measured by [3h]thymidine incorporation. the mitogen extract was derived from washed bacterial cells which were mechanically disrupted with a french press. the extract was centrifuged at 105,000 x g and filtered through a 0.22-micrometer filter. similar levels of lymphocyte stimulation were observed in lympho ...1978103837
the macrophage as an antineoplastic surveillance cell: biological perspectives. 1978104035
stimulation of human b lymphocytes by listeria cell wall fraction.cell wall fraction of listeria monocytogenes (lcwf), a b cell mitogen for mouse spleen cells, is also mitogenic for human adult and cord peripheral blood lymphocytes. purified b-cell suspensions responded to lcwf in vitro proliferation, to a similar extent as the unfractionated suspensions. furthermore, lcwf-induced b cell differentiation into igm-containing cells and their percentage correlated significantly with the extent of lymphocyte proliferation.1978104815
purification of antibodies to bacterial antigens by an immunoadsorbent and a method to quantify their reaction with insoluble bacterial targets.a combination of procedures was employed to develop a radioimmunoassay which quantified the binding of antibodies to antigens of either intact propionibacterium acnes or to antigens of insoluble extracts derived from the bacteria. reactive antibody populations were purified by use of bacterial immunoadsorbents which were prepared by coupling p. acnes to diethylaminoethyl cellulose. binding of antibodies was detected with [125i]staphylococcal protein a ([125i]spa) and optimal conditions for the a ...1979113462
[influence of maternal antibodies on the primary and secondary immunoresponse of the juvenile organism (author's transl)].the immunization of mice with sheep erythrocytes served as model for the materno-fetal transfer of specific antibodies. it could be shown that passively transferred antibodies are able to induce a long-lasting suppression of the primary and secondary immuno-response in the offspring. this holds true both for active immunization before pregnancy and for passive immunization shortly before term. as it can be supposed that similar effects also apply to the human organism, this phenomenon must be co ...1979105206
listeriosis in sheep. listeria monocytogenes excretion and immunological state in healthy sheep. 1979114002
[specific soluble antigens and listeria monocytogenes typing by electrosyneresis (author's transl)].listeria monocytogenes releases its antigens during the exponential phase. electrosyneresis on agarose allows detection of these antigens in the supernatant of broth culture following a 3 h incubation of the strains. this serotyping method seems to be easy, rapid and reproductible. the authors have investigated 83 strains of different origines and find no difference with agglutination. however, during the stationary phase, l. monocytogenes releases other antigens which gave precipitation lines w ...1979114080
microbial interference and colonization of the murine gastrointestinal tract by listeria monocytogenes.two strains of listeria monocytogenes, one that formed smooth colonies on agar surfaces and a varient of it that formed rough colonies, colonized the gastrointestinal tracts of germfree mice. within 24 h after mice were inoculated orally with about 100 bacteria, the population levels per gram (wet weight) of tissue of both strains were 10(5) to 10(7) in the stomach and ileum and 10(8) to 10(9) in the cecum and colon, respectively. as detected in gram-stained histological sections, in such gnotob ...1979106003
[listeria monocytogenes in newborn infants; an organism causing sepsis and meningitis]. 1979114867
enhancement of resistance to murine osteogenic sarcoma in vivo by an extract of brucella abortus (bru-pel): association with activation of reticuloendothelial system macrophages.the administration of an aqueous-ether extracted residue of brucella abortus (bru-pel) inhibits development of transplanted osteogenic sarcomas in mice as evidenced by a decrease in mortality. at least one mechanism through which bru-pel modulates host resistance is activation of macrophages of the reticuloendothelial system. peritoneal macrophages harvested from mice receiving bru-pel were cytotoxic for osteogenic sarcoma cells in vitro, limited the replication of vaccinia virus in cell culture ...1979106004
the effects of lipids from listeria monocytogenes on bacterial and fungal infections in mice.investigations were made on the influence of lipids from listeria monocytogenes upon the course of infections induced by bacteria and fungi. chloroform-methanol extract of the lipids was given to animals in various doses and at various intervals before and after infection. mortality of mice and elimination rate of bacteria from the blood and organs were estimated. it was observed that the lipids markedly increased natural immunity of the animals against bacterial and fungal infections. the morta ...1978107921
isolation of listeria monocytogenes from animals in the sudan. 1979107996
low temperature virulence of listeria monocytogenes in the avian embryo.the chick embryo was used to investigate the pathogenicity of listeria monocytogenes when inoculated into the air sac. one strain (nctc 5214) of l. monocytogenes was found to be more virulent for chick embryos when grown at low temperatures, while another strain (nctc 5105) did not produce this effect. this method was found to be reliable and reproducible and the chick embryo was found to be a more sensitive in vivo assay for virulence than the mouse.1979108871
response to therapy in an experimental rabbit model of meningitis due to listeria monocytogenes.a uniformly fatal, reproducible model of experimental meningitis due to listeria monocytogenes was developed in rabbits for study of the natural progression of the disease and was used to evaluate treatment regimens. bacterial titers in cerebrospinal fluid and pleocytosis approximated those found in humans. therapeutic studies in vivo revealed that rifampin was less rapidly bactericidal than ampicillin or penicillin, the agents usually recommended for treatment of meningitis; that penicillin plu ...1979115932
macrophage-t cell interactions involving listeria monocytogenes--role of the h-2 gene complex.this study reports on the in vitro interactions between t cells from listeria-immunized mice, macrophages from normal mice, and heat-killed listeria organisms. this interaction was assayed either by determing the amount of thymocyte mitogen in culture fluids after 24 hr, or by estimating the degree of t cell proliferation after 96 hr. each assay depended on critical concentrations of macrophages, t cells, and heat-killed listeria, points that were evaluated in a number of experiments. both assay ...1979109529
partial characterization of anti-tumor effector macrophages in the peritoneal cavities of concomitantly immune mice and mice injected with macrophage-stimulating agents. 1979115998
induction of cytocidal macrophages after in vitro interactions between listeria-immune t cells and macrophages--role of h-2. 1979109530
listeriosis in sheep. listeria monocytogenes excretion and immunological state in sheep in flocks with clinical listeriosis. 1979116488
isolation, characterization, and biological properties of an endotoxin-like material from the gram-positive organism listeria monocytogenes.the bacterial component responsible for the induction of transient cold agglutinin syndrome in rabbits after intravenous injection of heat-killed listeria monocytogenes type 4b has been purified and biologically and chemically characterized. a purified immunoglobulin m cold agglutinin was prepared from high-titer sera resulting from the immunization of rabbits with heat-killed l. monocytogenes type 4b and was subsequently used to monitor the purification of the bacterial component responsible fo ...1979110684
relationship between superoxide dismutase and pathogenic mechanisms of listeria monocytogenes.listeria monocytogenes was examined for superoxide dismutase(sod) activity. two catalase-negative strains possessed at least twofold greater sod activities than the catalase-positive l. monocytogenes strains examined. growth conditions such as aeration and iron concentration influenced the specific activity of sod obtained from cells cultured in defined media. l. monocytogenes sod from crude extracts and after partial purification was analyzed by polyacrylamide gel electrophoresis. iron was asso ...1979110685
susceptibility of cba/n mice to infection with salmonella typhimurium: influence of the x-linked gene controlling b lymphocyte function. 1979110879
morphology of liver lesions in experimental listeriosis is splenectomized mice. 1979110927
in vitro effect of mitogen-activated speen cells and their culture supernatants on mouse peritoneal macrophages as assayed by intracellular killing of listeria monocytogenes and content of beta-galactosidase. 1979110928
differing contribution of polymorphonuclear cells and macrophages to protection of mice against listeria monocytogenes and pseudomonas aeruginosa.bacterial growth and lethality of listeria monocytogenes in mice were augmented by carrageenan-treatment and x-irradiation (8 j kg-1), whereas growth and lethality of pseudomonas aeruginosa were augmented by x-irradiation but not by carrageenan-treatment. protection against l. monocytogenes, at least in the early phases, appears to depend mainly on macrophages, since carrageenan depletes macrophages but not polymorphonuclear cells (pmn), whereas protection against p. aeruginosa appears to depend ...1979119029
[two cases of meningitis due to listeria monocytogenes (author's transl)].two cases of meningitis due to listeria monocytogenes in a 73-year-old man and a 77-year-old woman are described. both patients were admitted to an emergency department in acute state, probably caused by cerebral stroke. neither presented clinical symptoms of meningitis. no other concomitant conditions which might weaken the patient's general state were known to be present. the first patient died after 16 hours, the second after 5. the analysis of the cerebrospinal fluid gave the following infor ...1979110989
immunostimulating effect of listeria monocytogenes, bcg and their biologically active extracts on the model of mouse ascites tumors. 1979120515
["listeria monocytogenes" isolation from a urine specimen of a subject with repeated abortions (author's transl)]. 1978111630
myocarditis with microabscess formation caused by listeria monocytogenes associated with myocardial infarct.myocardial infarction complicated by bacterial infection is rare. the present case is an instance in which the infecting organism, listeria monocytogenes, is also rare--an instance not previously reported. the clinical findings were fever without localized infection, severe atherosclerotic heart disease, a myocardial infarct of indeterminate age, and a left ventricular aneurysm. additional electrocardiographic findings include left bundle branch block, intraventricular conduction defect, and mul ...1979112029
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