| [lactobacillus vaginalis culture and its biochemical properties in vitro]. | | 1973 | 4755378 |
| [antigenic properties of lactobacillus vaginalis]. | | 1973 | 4765780 |
| lactobacillus panis sp. nov., from sourdough with a long fermentation period. | two lactobacillus strains isolated from rye sourdough most closely resemble lactobacillus oris on the basis of their physiological, morphological, and chemotaxonomic characteristics. a 16s ribosomal dna sequence analysis showed that these two strains clustered with, but were separate from, l. oris, lactobacillus vaginalis, and lactobacillus pontis. the results of dna-dna hybridization experiments indicated that the new two isolates represent a new lactobacillus species, for which we propose the ... | 1996 | 8934903 |
| [estimation of cytological smears in women with gardnerella vaginalis infections of vagina and uterine cervix]. | among micro-organisms infecting vagina whose dominant genus is gardnerella vaginalis anaerobic bacteria are often present. well-known damaging tissues and cells features of anaerobic bacteria are taken under consideration as factors which are conductive to generating pathological changes in cervical epithelium. the aim of this study was evaluation of cytological smears (pap smear, gram stain and h + e stain) in women with gardnerella vaginalis infections of vagina and uterine cervix. the researc ... | 1997 | 9780509 |
| multiplex pcr for the detection of lactobacillus pontis and two related species in a sourdough fermentation. | a specific multiplex pcr assay based on the amplification of parts of the 16s rrna molecule was designed. primers derived from variable regions of the 16s rrna provided a means of easily differentiating the species lactobacillus pontis and lactobacillus panis. they could be clearly discriminated from the phylogenetically related species lactobacillus vaginalis, lactobacillus oris, and lactobacillus reuteri and from other lactobacilli commonly known to be present in sourdough. other strains isola ... | 2000 | 10788389 |
| lactobacillus frumenti sp. nov., a new lactic acid bacterium isolated from rye-bran fermentations with a long fermentation period. | within the framework of the characterization of the microflora of an industrial sourdough fermentation, strains of lactobacillus amylovorus, lactobacillus pontis and two other strains were isolated which could not be associated with a valid species. these latter strains were gram-positive, catalase-negative, non-spore-forming, non-motile rods that could be clearly differentiated from known species by 16s rdna sequence analysis. for further characterization, the morphological, physiological (suga ... | 2000 | 11155988 |
| use of intravaginal microbicides to prevent acquisition of trichomonas vaginalis infection in lactobacillus-pretreated, estrogenized young mice. | d2a21, a novel peptide antibiotic has in vitro activity against a wide spectrum of sexually transmitted diseases (std). in this study we tested the hypothesis that intravaginal d2a21 would interfere with acquisition of trichomonas vaginalis infection in a modified mouse model. t. vaginalis infections of estrogenized young mice pretreated with lactobacillus vaginalis or lactobacillus rhamnosus were more frequent and persistent than those in mice pre-treated with lactobacillus gasseri or lactobaci ... | 2000 | 11421379 |
| [incidence of the l form of lactobacillus vaginalis (doederlein bacillus)]. | | 1956 | 13396791 |
| oral cavity as natural reservoir for intestinal lactobacilli. | ecological studies indicate that most lactobacillus species found in the human gastrointestinal tract are likely to be transient (allochthonous), originating from either the oral cavity or food. in order to investigate if oral lactobacilli constitute a part of the faecal lactobacillus biota, the lactobacillus biota of saliva and faeces of three human subjects were investigated and compared at two time-points in a 3-months interval. bacteriological culture, performed by incubation under standard ... | 2006 | 16423658 |
| influence of intensive and extensive breeding on lactic acid bacteria isolated from gallus gallus domesticus ceca. | in the present study, lactic acid bacteria (lab) from the cecum of chickens bred either under intensive (commercial broilers) or extensive (free-range) conditions were isolated, identified and some of their probiotic characteristics determined. the lab identified by 16s-23s rrna pcr-ardra were mainly of lactobacillus species and to a lesser extent of enterococcus spp. for all animals. free-range chickens showed a higher presence of lactobacillus acidophilus while lactobacillus reuteri and lactob ... | 2007 | 17113730 |
| diversity of lactobacilli in the oral cavities of young women with dental caries. | for nearly a century, lactobacilli (lb) in the oral cavity have been generally associated with dental caries. here, we characterized the lb isolated from the saliva of 6 women with active caries using genetic-based taxonomical identification methods. from each subject, 30 isolates growing on rogosa medium and presumed to be lb were analyzed. of the 180 isolates, 176 were further characterized by biotyping, dna melting points, dna chromosomal fingerprinting, genotyping, and phylogenetic cluster a ... | 2007 | 17167253 |
| species diversity and relative abundance of vaginal lactic acid bacteria from women in uganda and korea. | lactobacilli play an important role in maintaining vaginal health of women. the aim of this study was to compare the species richness and relative abundance of lactobacillus and other lactic acid bacteria in women of two geographically distant countries, uganda and korea. | 2007 | 17381754 |
| production of exopolysaccharides by lactobacillus and bifidobacterium strains of human origin, and metabolic activity of the producing bacteria in milk. | this work reports on the physicochemical characterization of 21 exopolysaccharides (eps) produced by lactobacillus and bifidobacterium strains isolated from human intestinal microbiota, as well as the growth and metabolic activity of the eps-producing strains in milk. the strains belong to the species lactobacillus casei, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus vaginalis, bifidobacterium animalis, bifidobacterium longum, and bifidobacterium pseudocatenulatum. the molar ma ... | 2009 | 19700676 |
| microbial risk markers for childhood caries in pediatricians' offices. | dental caries in pre-school children has significant public health and health disparity implications. to determine microbial risk markers for this infection, this study aimed to compare the microbiota of children with early childhood caries with that of caries-free children. plaque samples from incisors, molars, and the tongue from 195 children attending pediatricians' offices were assayed by 74 dna probes and by pcr to streptococcus mutans. caries-associated factors included visible plaque, chi ... | 2010 | 20164496 |
| lactobacillus strain ecology and persistence within broiler chickens fed different diets: identification of persistent strains. | lactobacilli are autochthonous residents in the chicken gastrointestinal tract, where they may potentially be used as probiotics, competitive exclusion agents, or delivery vehicles. the aim of this study was to use an in vivo model to investigate the effect of diet and competing lactic acid bacteria on the colonization of inoculated lactobacillus strains, with the goal of identifying strains which can consistently colonize or persist for an extended period of several weeks. chicken-derived lacto ... | 2010 | 20693442 |
| diversity of lactobacillus and bifidobacterium in feces of herbivores, omnivores and carnivores. | the lactobacillus and bifidobacterium population in the feces of 26 animals (16 species) were studied by culture-dependent and culture-independent techniques. lactobacilli were detected from a few herbivores, all carnivores and some omnivores. lactobacillus johnsonii, lactobacillus reuteri, lactobacillus salivarius, lactobacillus vaginalis and lactobacillus ingluviei were the most dominant lactobacilli in carnivores. these species were, however, not predominant in herbivores and omnivores. lacto ... | 2010 | 21034840 |
| transformation of, and heterologous protein expression in, lactobacillus agilis and lactobacillus vaginalis isolates from the chicken gastrointestinal tract. | lactobacilli are naturally found in the gastrointestinal tract of chickens, and there is interest in utilizing autochthonous strains for the delivery of therapeutic proteins. previously we identified three chicken-derived lactobacillus strains, lactobacillus agilis la3, lactobacillus vaginalis lv5, and lactobacillus crispatus lc9, which persist in the gastrointestinal tract of chickens fed either a commercial or high-protein diet. in the current study, we investigated the ability to electrotrans ... | 2010 | 21075881 |
| growth characterization of individual rye sourdough bacteria by isothermal microcalorimetry. | the present work tests the feasibility of the isothermal microcalorimetry method to study the performance of individual lactic acid bacteria during solid-state fermentation in rye sourdough. another aim was to elucidate the key factors leading to the formation of different microbial consortia in laboratory and industrial sourdough during continuous backslopping propagation. | 2010 | 21143710 |
| an in vitro protocol for direct isolation of potential probiotic lactobacilli from raw bovine milk and traditional fermented milks. | a method for isolating potential probiotic lactobacilli directly from traditional milk-based foods was developed. the novel digestion/enrichment protocol was set up taking care to minimize the protective effect of milk proteins and fats and was validated testing three commercial fermented milks containing well-known probiotic lactobacillus strains. only probiotic bacteria claimed in the label were isolated from two out of three commercial fermented milks. the application of the new protocol to 1 ... | 2011 | 21318359 |
| biodiversity of lactic acid bacteria and yeasts in spontaneously-fermented buckwheat and teff sourdoughs. | in this study, four different laboratory scale gluten-free (gf) sourdoughs were developed from buckwheat or teff flours. the fermentations were initiated by the spontaneous biota of the flours and developed under two technological conditions (a and b). sourdoughs were propagated by continuous back-slopping until the stability was reached. the composition of the stable biota occurring in each sourdough was assessed using both culture-dependent and -independent techniques. overall, a broad spectru ... | 2010 | 21356457 |
| screening of lactic acid bacteria and bifidobacteria for potential probiotic use in iberian dry fermented sausages. | the purpose of this study was to select lactic acid bacteria and bifibobacteria strains as potential probiotic cultures during the processing of iberian dry fermented sausages. a total of 1000 strains were isolated from iberian dry fermented sausages (363), and human (337) and pig faeces (300) in different culture media. around 30% of these strains, mainly isolated from iberian dry fermented sausages in lamvab agar, were pre-selected for testing as potential probiotics by their ability to grow a ... | 2008 | 22063588 |
| cross-sectional analysis of selected genital tract immunological markers and molecular vaginal microbiota in sub-saharan african women, with relevance to hiv risk and prevention. | data on immune mediators in the genital tract and the factors that modulate them in sub-saharan women are limited. cervicovaginal lavage (cvl) samples from 430 sexually active women from kenya, south africa, and rwanda were analyzed for 12 soluble immune mediators using bio-plex and meso scale discovery multiplex platforms, as well as single enzyme-linked immunosorbent assays. ten bacterial species were quantified in vaginal swab samples. bacterial vaginosis (bv) was defined by nugent scoring. c ... | 2015 | 25761460 |
| establishment of a sensitive system for analysis of human vaginal microbiota on the basis of rrna-targeted reverse transcription-quantitative pcr. | ten specific primer sets, for lactobacillus gasseri, lactobacillus crispatus, atopobium vaginae, gardnerella vaginalis, mobiluncus curtisii, chlamydia trachomatis/muridarum, bifidobacterium longum subsp. longum, bifidobacterium longum subsp. infantis, bifidobacterium adolescentis, and bifidobacterium angulatum, were developed for quantitative analysis of vaginal microbiota. rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) analysis of the vaginal samples from 12 healthy japanese vol ... | 2015 | 25661498 |
| identification and characterization of bacterial vaginosis-associated pathogens using a comprehensive cervical-vaginal epithelial coculture assay. | bacterial vaginosis (bv) is the most commonly treated female reproductive tract affliction, characterized by the displacement of healthy lactobacilli by an overgrowth of pathogenic bacteria. bv can contribute to pathogenic inflammation, preterm birth, and susceptibility to sexually transmitted infections. as the bacteria responsible for bv pathogenicity and their interactions with host immunity are not understood, we sought to evaluate the effects of bv-associated bacteria on reproductive epithe ... | 2012 | 23166828 |
| biofilm-forming capacity in biogenic amine-producing bacteria isolated from dairy products. | biofilms on the surface of food industry equipment are reservoirs of potentially food-contaminating bacteria-both spoilage and pathogenic. however, the capacity of biogenic amine (ba)-producers to form biofilms has remained largely unexamined. bas are low molecular weight, biologically active compounds that in food can reach concentrations high enough to be a toxicological hazard. fermented foods, especially some types of cheese, accumulate the highest ba concentrations of all. the present work ... | 2016 | 27242675 |
| characteristics of lactobacillus and gardnerella vaginalis from women with or without bacterial vaginosis and their relationships in gnotobiotic mice. | the objectives of the present study were to evaluate in vitro the production of antagonistic compounds against gardnerella vaginalis by lactobacillus strains isolated from women with or without bacterial vaginosis (bv), and to select one of the better lactobacillus producers of such a substance to be tested in vivo using a gnotobiotic animal model challenged with one of the more sensitive g. vaginalis isolates. a total of 24 isolates from women with and without bv were identified as g. vaginalis ... | 2012 | 22539000 |
| probiotic and technological properties of lactobacillus spp. strains from the human stomach in the search for potential candidates against gastric microbial dysbiosis. | this work characterizes a set of lactobacilli strains isolated from the stomach of healthy humans that might serve as probiotic cultures. ten different strains were recognized by rep-pcr and pfge fingerprinting among 19 isolates from gastric biopsies and stomach juice samples. these strains belonged to five species, lactobacillus gasseri (3), lactobacillus reuteri (2), lactobacillus vaginalis (2), lactobacillus fermentum (2) and lactobacillus casei (1). all ten strains were subjected to a series ... | 2014 | 25642213 |
| transfer of antibiotic resistance determinants between lactobacilli isolates from the gastrointestinal tract of chicken. | the aim of this study was to assess the potential horizontal transfer of genetic traits for antibiotic resistance between lactobacilli isolated from the chicken gut, both in vitro and in vivo. thirty-seven lactobacillus spp. strains isolated from the gizzard, small and large intestines and caeca of free-range broiler chickens showed multi-drug resistance as assessed by disc diffusion assays. the minimum inhibitory concentration (mic) for vancomycin, tetracycline, erythromycin and chloramphenicol ... | 2012 | 22476322 |
| naturally occurring lactic acid bacteria isolated from tomato pomace silage. | silage making has become a significant method of forage conservation worldwide. to determine how tomato pomace (tp) may be used effectively as animal feed, it was ensilaged for 90 days and microbiology counts, fermentation characteristics and chemical composition of tomato pomace silage (tps) were evaluated at the 30th, 60th, and 90th days, respectively. in addition, 103 lactic acid bacteria were isolated from tps. based on the phenotypic and chemotaxonomic characteristics, 16s rdna sequence and ... | 2014 | 25049999 |
| determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products. | functional foods could differently affect human health in relation to the gender. recent studies have highlighted the anti-candida and anti-chlamydia activities of some lactobacillus strains isolated from the vagina of healthy women. considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. in this context, the aim of this work was to study some f ... | 2017 | 28223974 |
| a dna tool for early detection of vaginal dysbiosis in african women. | a next-generation diagnostic tool for bacterial vaginosis, consisting of quantitative and/or qualitative molecular criteria, has not yet been identified. the optimal diagnostic tool should not only diagnose bacterial vaginosis in diverse populations, but should also detect early signs of transition to dysbiosis. we evaluated a tool based on log10-transformed qpcr data for lactobacillus crispatus, lactobacillus iners, lactobacillus jensenii, lactobacillus gasseri, lactobacillus vaginalis, lactoba ... | 2016 | 26577657 |
| acid production and growth by oral lactobacillus species in vitro. | to analyze the acid-producing and growth abilities of different oral lactobacillus species. | 2011 | 22298522 |
| Immunotherapy of cervical cancer as a biological dissipative structure. | Cervical cancer can be not only prevented, but also effectively treated. Decreased efficiency of biochemical, neurohormonal and/or immunological mechanisms leads to infectious states which, irrespective of their bacterial, viral or parasitic aetiology, are only the necessary, but not the sufficient causes of neogenesis. The cause of cancer is the natural and common phenomenon of the self-organization of systems, endangered by ending of their existence, into more efficient time-space structures a ... | 2011 | 21876504 |
| Effect of grape polyphenols on lactic acid bacteria and bifidobacteria growth: Resistance and metabolism. | Food polyphenols are able to selectively modify the growth of susceptible micro-organisms. This study describes the effect of a flavan-3-ol enriched grape seed extract (GSE) on the growth of several lactic acid bacteria (LAB) and bifidobacteria and the ability of the resistant strains to metabolize these compounds. Streptococcus thermophilus, Lactobacillus fermentum, Lactobacillus acidophilus and Lactobacillus vaginalis strains showed a remarkable sensitivity to the phenolic extracts assayed, in ... | 2011 | 21839384 |
| inhibitory effect of oral lactobacillus against oral pathogens. | aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ... | 2011 | 21801186 |
| (gtg)(5)-pcr fingerprinting of lactobacilli isolated from cervix of healthy women. | a group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (gtg)(5)-polymerase chain reaction (pcr) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. obtained fingerprints were compared with profiles available in an in-house database of the ccm bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. selected strains representing individual clusters were further identi ... | 2011 | 21390569 |
| identification of predominant culturable vaginal lactobacillus species and associated bacteriophages from women with and without vaginal discharge syndrome in south africa. | lactobacillus jensenii, lactobacillus crispatus, lactobacillus iners, lactobacillus gasseri and lactobacillus vaginalis were identified by 16s rrna gene sequencing as the predominant culturable vaginal lactobacillus species in a group of south african women, comprising 24, 22, 10, 10 and 9 %, respectively. a significant effect of vaginal discharge syndrome (vds) and bacterial vaginosis (bv) on the distribution of predominant lactobacillus species was observed. whilst l. crispatus isolates were a ... | 2010 | 21030503 |
| [influence of hormonal replacement therapy on bacterial vaginosis in the group of peri- and postmenopausal women]. | the aim of the study was to evaluate microbiological biocenosis of the menopausal or postmenopausal women, and women having local hormonal therapy (ovestin and ortho-gynest d). the study group consisted of 123 women (aged 45-72), who underwent clinical examination and microbiological evaluation of vaginal discharge. miller's and gramm's methods were used for slide staining and the culture method for growing microorganisms. classical culture media were used as well as rogosa culture medium and mo ... | 2001 | 11848019 |
| [microbiological evaluation of the vagina in women being screened for inflammatory and precancerous conditions of female genitalia]. | the aim of this work was to study the microbiology of vaginal secretion in 3665 (19 to 72 year old) women investigated in the screening programme for inflammatory and malignant diseases. microbiological examinations of the vaginal contents were carried out using direct preparations (miller and gram methods) and different media for cultures. normal biocenosis (i and ii grade of cleanliness) of the vagina was observed in 40.74% investigated patients. abnormal biocenosis was classified as early, ad ... | 1999 | 10910658 |
| [presence and morphological properties of lactobacillus vaginalis at different periods of the development of female body]. | | 1973 | 4742020 |
| interaction of vaginal lactobacillus strains with hela cells plasma membrane. | vaginal lactobacilli offer protection against recurrent urinary and vaginal infections. the precise mechanisms underlying the interaction between lactobacilli and the host epithelium remain poorly understood at the molecular level. deciphering such events can provide valuable information on the mode of action of commensal and probiotic bacteria in the vaginal environment. we investigated the effects exerted by five lactobacillus strains of vaginal origin (lactobacillus crispatus bc1 and bc2, lac ... | 2017 | 28618863 |
| the genome of the lactobacillus sanfranciscensis temperate phage ev3. | bacteriophages infection modulates microbial consortia and transduction is one of the most important mechanism involved in the bacterial evolution. however, phage contamination brings food fermentations to a halt causing economic setbacks. the number of phage genome sequences of lactic acid bacteria especially of lactobacilli is still limited. we analysed the genome of a temperate phage active on lactobacillus sanfranciscensis, the predominant strain in type i sourdough fermentations. | 2013 | 24308641 |
| the composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women. | this study was undertaken to characterize the vaginal microbiota throughout normal human pregnancy using sequence-based techniques. we compared the vaginal microbial composition of non-pregnant patients with a group of pregnant women who delivered at term. | 2014 | 24484853 |
| isolation and typification of histamine-producing lactobacillus vaginalis strains from cheese. | in food, the biogenic amine (ba) histamine is mainly produced by histidine decarboxylation catalysed by microbial histidine decarboxylase. the consumption of foods containing high concentrations of histamine can trigger adverse neurological, gastrointestinal and respiratory reactions. indeed, histamine is one of the most toxic of all bas, and is often detected in high concentration in cheese. however, little is known about the microorganisms responsible for its accumulation in this food. in the ... | 2015 | 26394683 |
| genomic characterization of a fructophilic bee symbiont lactobacillus kunkeei reveals its niche-specific adaptation. | lactobacillus kunkeei is classified as a sole obligate fructophilic lactic acid bacterium that is found in fructose-rich niches, including the guts of honeybees. the species is differentiated from other lactobacilli based on its poor growth with glucose, enhanced growth in the presence of oxygen and other electron acceptors, and production of high concentrations of acetate from the metabolism of glucose. these characteristics are similar to phylogenetically distant fructobacillus spp. in the pre ... | 2016 | 27776911 |