| effects of lasalocid or monensin on lactate-producing or -using rumen bacteria. | lasalocid or monensin inhibited most of the lactate-producing rumen bacteria (butyrivibrio fibrisolvens, eubacterium cellulosolvens, e. ruminantium, lachnospira multiparus, lactobacillus ruminis, l. vitulinus, ruminococcus albus, r. flavefaciens, streptococcus bovis). minimum inhibitory concentrations ranged from .38 to 3.0 micrograms/ml. among the lactate producers, those that produce succinate as a major end product (bacteroides, selenomonas, succinimonas, succinivibrio) were not inhibited by ... | 1981 | 7275867 |
| lactobacillus ruminis strains cluster according to their mammalian gut source. | lactobacillus ruminis is a motile lactobacillus that is autochthonous to the human gut, and which may also be isolated from other mammals. detailed characterization of l. ruminis has previously been restricted to strains of human and bovine origin. we therefore sought to expand our bio-bank of strains to identify and characterise isolates of porcine and equine origin by comparative genomics. | 2015 | 25879663 |
| gut dysbiosis is associated with metabolism and systemic inflammation in patients with ischemic stroke. | the role of metabolic diseases in ischemic stroke has become a primary concern in both research and clinical practice. increasing evidence suggests that dysbiosis is associated with metabolic diseases. the aim of this study was to investigate whether the gut microbiota, as well as concentrations of organic acids, the major products of dietary fiber fermentation by the gut microbiota, are altered in patients with ischemic stroke, and to examine the association between these changes and host metab ... | 2017 | 28166278 |
| a comparative characterization of different host-sourced lactobacillus ruminis strains and their adhesive, inhibitory, and immunomodulating functions. | lactobacillus ruminis, an autochthonous member of the gastrointestinal microbiota of humans and many animals, is a less characterized but interesting species for many reasons, including its intestinal prevalence and possible positive roles in host-microbe crosstalk. in this study, we isolated a novel l. ruminis strain (grl 1172) from porcine feces and analyzed its functional characteristics and niche adaptation factors in parallel with those of three other l. ruminis strains (a human isolate, at ... | 2017 | 28450859 |
| identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten. | identify and characterize bacteria from the proximal gastrointestinal tract of pigs capable of degrading immunogenic gluten peptides. | 2015 | 25376327 |
| characterization of flagellins isolated from a highly motile strain of lactobacillus agilis. | most lactic acid bacteria are non-motile but some of them are flagellated and exhibit motility. so far, motile lactobacilli have rarely been studied, and characteristics of their flagellins are poorly understood. in this study, a highly motile strain of lactobacillus agilis was recruited for transcriptional analysis and characterization of its flagellins. | 2016 | 27001290 |
| characterization of pro-inflammatory flagellin proteins produced by lactobacillus ruminis and related motile lactobacilli. | lactobacillus ruminis is one of at least twelve motile but poorly characterized species found in the genus lactobacillus. of these, only l. ruminis has been isolated from mammals, and this species may be considered as an autochthonous member of the gastrointestinal microbiota of humans, pigs and cows. nine l. ruminis strains were investigated here to elucidate the biochemistry and genetics of lactobacillus motility. six strains isolated from humans were non-motile while three bovine isolates wer ... | 2012 | 22808200 |
| antiviral effects of lactobacillus ruminis spm0211 and bifidobacterium longum spm1205 and spm1206 on rotavirus-infected caco-2 cells and a neonatal mouse model. | rotavirus is worldwide cause of severe gastroenteritis including severe diarrhea and fatal dehydration in infants and young children. there is an available vaccination program for preventing rotavirus infection, but it has limits and restrictions. probiotics therapy could be an alternative method of antiviral prevention and modulation against rotavirus infection. in this study, we screened the antiviral activity of probiotic bacteria such as 3 lactobacillus spp. and 14 bifidobacterium spp. isola ... | 2015 | 26502964 |
| gut microbiota in malawian infants in a nutritional supplementation trial. | to examine whether two forms of lipid-based nutrient supplements (lns) or a micronutrient-fortified corn-soya blend were associated with development of the gut microbiota in malawian infants, to assess the microbiota profiles at the age of 6 and 18 months and to follow the changes during the 12-month period. | 2016 | 26644222 |
| anti-inflammatory and intestinal barrier-protective activities of commensal lactobacilli and bifidobacteria in thoroughbreds: role of probiotics in diarrhea prevention in neonatal thoroughbreds. | we previously isolated the commensal bacteria lactobacilli and bifidobacteria from the thoroughbred intestine and prepared the horse probiotics lacfi(tm), consisting of lactobacillus ruminis kk14, l. equi kk 15, l. reuteri kk18, l. johnsonii kk21, and bifidobacterium boum hu. here, we found that the five lacfi(tm) constituent strains remarkably suppressed pro-inflammatory interleukin-17 production in mouse splenocytes stimulated with interleukin-6 and transforming growth factor-β. the protective ... | 2014 | 25013357 |
| human gut-commensalic lactobacillus ruminis atcc 25644 displays sortase-assembled surface piliation: phenotypic characterization of its fimbrial operon through in silico predictive analysis and recombinant expression in lactococcus lactis. | sortase-dependent surface pili (or fimbriae) in gram-positive bacteria are well documented as a key virulence factor for certain harmful opportunistic pathogens. however, it is only recently known that these multi-subunit protein appendages are also belonging to the "friendly" commensals and now, with this new perspective, they have come to be categorized as a niche-adaptation factor as well. in this regard, it was shown earlier that sortase-assembled piliation is a native fixture of two human i ... | 2015 | 26709916 |
| carbohydrate staple food modulates gut microbiota of mongolians in china. | gut microbiota is a determining factor in human physiological functions and health. it is commonly accepted that diet has a major influence on the gut microbial community, however, the effects of diet is not fully understood. the typical mongolian diet is characterized by high and frequent consumption of fermented dairy products and red meat, and low level of carbohydrates. in this study, the gut microbiota profile of 26 mongolians whom consumed wheat, rice and oat as the sole carbohydrate stapl ... | 2017 | 28377764 |
| evaluation of colonisation resistance in stool of human donors using ex vivo, in vitro and in vivo assays. | the indigenous microbiota is the population of microorganisms normally present on the surface and mucosa of an individual, where it performs essential health functions, including the colonisation resistance (cr) against pathogens. to identify the bacteria responsible and the mechanisms involved in the cr, the germ-free (gf) animal model has been used, because in vitro studies cannot always be extrapolated to what occurs in vivo. in this study, ex vivo antagonism assays against seven enteropathog ... | 2017 | 28116929 |
| carbohydrate catabolic flexibility in the mammalian intestinal commensal lactobacillus ruminis revealed by fermentation studies aligned to genome annotations. | lactobacillus ruminis is a poorly characterized member of the lactobacillus salivarius clade that is part of the intestinal microbiota of pigs, humans and other mammals. its variable abundance in human and animals may be linked to historical changes over time and geographical differences in dietary intake of complex carbohydrates. | 2011 | 21995520 |
| Characterization of tetracycline resistance lactobacilli isolated from swine intestines at western area of Taiwan. | To investigate the frequency of tetracycline resistance (Tet-R) lactobacilli in pig intestines, a total of 256 pig colons were analyzed and found to contain typical colonies of Tet-R lactic acid bacteria in every sample, ranging from 3.2 × 10(3) to 6.6 × 10(5) CFU/cm(2). From these samples, a total of 159 isolates of Tet-R lactobacilli were obtained and identified as belonging to 11 species, including Lactobacillus reuteri, Lactobacillus amylovorus, Lactobacillus salivarius, Lactobacillus planta ... | 2011 | 21906691 |
| genome sequence of lactobacillus ruminis spm0211, isolated from a fecal sample from a healthy korean. | lactobacillus ruminis spm0211 is a potential probiotic strain that shows antimicrobial activity against emerging pathogens. here we present the draft genomic sequence of l. ruminis spm0211, isolated from a fecal sample from a healthy korean and describe both the common and unique features of this strain. | 2011 | 21742873 |
| lactobacillus saerimneri and lactobacillus ruminis: novel human-derived probiotic strains with immunomodulatory activities. | human-derived lactobacilli were isolated from fecal samples of healthy volunteers. forty-six isolates from different volunteers were selected and investigated for their immunomodulatory properties. conditioned medium from each isolate was assessed for its effect on tumor necrosis factor (tnf) production in lipopolysaccharide-activated thp-1 monocytes. of 46 lactobacillus isolates, 12 significantly inhibited tnf production in varying magnitude. lactobacillus strain th58 displayed the most potent ... | 2009 | 19222575 |
| biotransformation of 2,4,6-trinitrotoluene by pure culture ruminal bacteria. | twenty-one ruminal bacteria species were tested for their ability to degrade 2,4,6-trinitrotoluene (tnt) within 24 h. butyrivibrio fibrisolvens, fibrobacter succinogenes, lactobacillus vitulinus, selenomonas ruminantium, streptococcus caprinus, and succinivibrio dextrinosolvens were able to completely degrade 100 mg/l tnt, with <5% of the original tnt recovered as diaminonitrotoluene metabolites. eubacterium ruminantium, lactobacillus ruminis, ruminobacter amylophilus, streptococcus bovis, and w ... | 2009 | 18839246 |
| isolation and identification of the dominant lactobacillus in gut and faeces of pigs using carbohydrate fermentation and 16s rdna analysis. | the gut contents and faeces of 9 adult pigs and 387 colonies growing on mrs culture (selective for lactobacillus) were sampled to isolate and identify lactobacillus species present. the results showed that the number of bacteria growing on mrs culture increased from the anterior to posterior of the gut and decreased in the faeces (e.g., stomach, 4.04 x 10(5) cfu/g; small intestine, 9.77 x 10(7) cfu/g; large intestine 3.85 x 10(11) cfu/g; faeces, 1.22 x 10(11) cfu/g). fifty-two lactobacilli-like ... | 2005 | 16233756 |
| variation of microbiological and biochemical parameters in the faeces of two healthy people over a 15 day period. | recent years have seen increasing interest in the complex microbial ecosystem of the human gastrointestinal tract. knowledge of its microbial colonists and their beneficial/detrimental activities is important. however, generalized assumptions about the microbial composition of the human gut should be taken with caution until more studies in different human communities have been conducted. the capacity of modulating or inhibiting harmful populations through high doses of beneficial microorganisms ... | 2004 | 15597174 |
| lactobacillus ruminis is a predominant lactic acid producing bacterium in the caecum and rectum of the pig. | to identify the predominant lactic acid producing bacteria in the small intestine, caecum and the rectum of the healthy pig. | 2003 | 12904222 |
| diet influences the ecology of lactic acid bacteria and escherichia coli along the digestive tract of cattle: neural networks and 16s rdna. | in this manuscript, the authors have sought to gain a better understanding of the interactions between escherichia coli and lactic acid bacteria (lab) isolated from rogossa mrs agar along the digestive tract of grain- and forage-fed cattle. e. coli from cattle receiving a high-grain diet were more numerous (p<0.05) than from the high-forage diet and the highest numbers were in the faeces. isolates on rogossa mrs agar were always greater in the high-grain diet (p<0.05) and contained a significant ... | 2003 | 12576580 |
| whole-transcriptome shotgun sequencing (rna-seq) screen reveals upregulation of cellobiose and motility operons of lactobacillus ruminis l5 during growth on tetrasaccharides derived from barley β-glucan. | lactobacillus ruminis is an inhabitant of human bowels and bovine rumens. none of 10 isolates (three from bovine rumen, seven from human feces) of l. ruminis that were tested could utilize barley β-glucan for growth. seven of the strains of l. ruminis were, however, able to utilize tetrasaccharides (3-o-β-cellotriosyl-d-glucose [ldp4] or 4-o-β-laminaribiosyl-d-cellobiose [cdp4]) present in β-glucan hydrolysates for growth. the tetrasaccharides were generated by the use of lichenase or cellulase, ... | 2013 | 23851085 |
| distinctive intestinal lactobacillus communities in 6-month-old infants from rural malawi and southwestern finland. | our aim was to compare the composition and diversity of lactobacillus microbiota in infants living in malawi and southwestern finland. | 2015 | 26049782 |
| genome sequences and comparative genomics of two lactobacillus ruminis strains from the bovine and human intestinal tracts. | the genus lactobacillus is characterized by an extraordinary degree of phenotypic and genotypic diversity, which recent genomic analyses have further highlighted. however, the choice of species for sequencing has been non-random and unequal in distribution, with only a single representative genome from the l. salivarius clade available to date. furthermore, there is no data to facilitate a functional genomic analysis of motility in the lactobacilli, a trait that is restricted to the l. salivariu ... | 2011 | 21995554 |
| changes in faecal bacteria associated with concentrate and forage-only diets fed to horses in training. | diets rich in readily fermentable carbohydrates, fed traditionally to meet the increased energy requirements of the performance horse, are associated with a number of gastrointestinal disorders that involve disturbances in the intestinal microbiota, however, these changes are poorly understood. | 2009 | 20383990 |
| molecular diversity of lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16s ribosomal dna. | a lactobacillus group-specific pcr primer, s-g-lab-0677-a-a-17, was developed to selectively amplify 16s ribosomal dna (rdna) from lactobacilli and related lactic acid bacteria, including members of the genera leuconostoc, pediococcus, and weissella: amplicons generated by pcr from a variety of gastrointestinal (gi) tract samples, including those originating from feces and cecum, resulted predominantly in lactobacillus-like sequences, of which ca. 28% were most similar to the 16s rdna of lactoba ... | 2002 | 11772617 |
| two new species of lactobacillus isolated from the bovine rumen, lactobacillus ruminis sp.nov. and lactobacillus vitulinus sp.nov. | | 1973 | 4723944 |
| an in silico pan-genomic probe for the molecular traits behind lactobacillus ruminis gut autochthony. | as an ecological niche, the mammalian intestine provides the ideal habitat for a variety of bacterial microorganisms. purportedly, some commensal genera and species offer a beneficial mix of metabolic, protective, and structural processes that help sustain the natural digestive health of the host. among these sort of gut inhabitants is the gram-positive lactic acid bacterium lactobacillus ruminis, a strict anaerobe with both pili and flagella on its cell surface, but also known for being autocht ... | 2017 | 28414739 |
| novel molecular insights about lactobacillar sortase-dependent piliation. | one of the more conspicuous structural features that punctuate the outer cell surface of certain bacterial gram-positive genera and species is the sortase-dependent pilus. as these adhesive and variable-length protrusions jut outward from the cell, they provide a physically expedient and useful means for the initial contact between a bacterium and its ecological milieu. the sortase-dependent pilus displays an elongated macromolecular architecture consisting of two to three types of monomeric pro ... | 2017 | 28718795 |