| urease-producing species of intestinal anaerobes and their activities. | urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ... | 1979 | 36839 |
| [role of normal microflora in the treatment of vibrio carrier state in rat gnotobionts]. | materials on the study of interactions between vibrio cholera el tor and some indigenous bacteria of human intestinal tract, which proved to be active antagonists in vitro, are presented in this work. experiments were conducted on gnotobiotic rats (fisher), using various combinations and variants of the order of the microorganism administration; there were revealed no antagonistic relations between virbrio cholerae el tor and escherichia coli m-17, lactobacillus fermenti, lactobacillus plantarum ... | 1978 | 352437 |
| serological studies on chemostat-grown cultures of lactobacillus fermentum and lactobacillus plantarum. | lactobacillus fermentum nctc 6991 and lactobacillus plantarum ncib 7220 were grown in a chemostat in the diffusible fraction of complex medium at ph 6.0 with glucose limitation. organisms grown at different dilution rates (d) were injected into rabbits, and the resultant antisera were examined for reactivity with antigens previously isolated from batch-grown organisms. for l. fermentum, antisera obtained on injecting cells grown at d = 0.5 h-1 contained a significantly higher level of antibody r ... | 1979 | 457268 |
| [influence of some thiamine antagonists on the growth of kloeckera apiculata, lactobacillus fermenti and lactobacillus arabinosus (author's transl)]. | | 1977 | 557103 |
| bacteria associated with the gastric epithelium of neonatal pigs. | light and electron microscopy showed lactobacilli and, to a lesser degree, streptococci to be closely associated with the squamous area of the pig stomach known as the pars esophagea. several different types of extracellular layers were seen on bacteria attached to the epithelial surface. the total number of bacteria per square centimeter did not change with age up to 10 days, and there was no effect of weaning at 2 days. lactobacillus fermentum, l. salivarius, and streptococcus salivarius were ... | 1978 | 637551 |
| origin of esterases in human whole saliva. | whole saliva of 59 healthy persons was used for determination of esterase activity. the pattern of esterase was studied by means of isoelectrofocusing on thin-layer acrylamide gels. the esterases found in whole saliva are suggested to be derived from the cells of the tissue in the oral cavity. this origin is indicated (e.g.) by comparison between isolectrophoretic esterase patterns of whole saliva, submandibular saliva, gingival biopsy and fibroblast culture. antisera against partially purified ... | 1977 | 862601 |
| penicillin resistance and its elimination by treatment with acriflavine in lactobacillus fermenti. | | 1976 | 966394 |
| characterization of group n streptococcus lipoteichoic acid. | lipoteichoic acid was extracted from the group n organism streptococcus lactis atcc 9936 with hot aqueous phenol and purified by gel chromatography followed by affinity chromatography using ricinus communis lectin as the specific absorbent. the teichoic acid moiety of the lipoteichoic acid was calculated to contain 16 to 17 glycerol phosphate units, approximately half of which were substituted with alpha-d-galactosyl residues; the glycolipid moiety contained o-alpha-d-glucosyl-1 yields 2-o-alpha ... | 1975 | 1123256 |
| formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli. | examination of the culture fluids from a number of strains of oral streptococci and latobacilli has shown the presence of an erythrocyte-sensitizing antigen with the properties of lipoteichoic acid. the antigen was isolated from the culture fluids of lactobacillus casei and lactobacillus fermentum and characterized chemically and serologically, for other strains, serological evidence for the presence of lipoteichoic acid depends on the reactivity with antiserum specific for the glycerol phosphat ... | 1975 | 1158524 |
| a note on the use of a plasmid as a dna probe in the detection of a lactobacillus fermentum strain in porcine stomach contents. | a plasmid (about 50 kb) was used as a dna probe to enumerate, by colony hybridization, a strain of lactobacillus fermentum in the stomach contents of eight piglets. the population sizes obtained by colony hybridization were in agreement with estimated levels calculated on the basis of plasmid profiling of colonies isolated at random from the total lactobacillus population. | 1992 | 1512179 |
| existence of phosphoenolpyruvate: carbohydrate phosphotransferase systems in lactobacillus fermentum, an obligate heterofermenter. | the presence or absence of the phosphoenolpyruvate: carbohydrate phosphotransferase system (pts) in obligately heterofermentative group iii lactobacilli including lactobacillus brevis (3 strains), l. buchneri (2 strains) and l. fermentum (3 strains) was surveyed systematically for a series of sugars utilizable by these organisms. contrary to common expectation, ptss were found in two strains of l. fermentum: sucrose-pts in one strain; sucrose- and mannose-ptss in the other. all these activities ... | 1992 | 1513267 |
| microbiological methods of thiamine measurement in biological material. | semiautomated, microbiological methods for the determination of thiamine in a variety of biological material are presented. thiamine content was measured in bovine, ovine and rat blood, plasma, bile, urine, duodenal fluid, liver, kidney and brain. thiamine was extracted from samples by mild acid digestion (trans aconitate ph 4.0) at 115 degrees c for 20 min. microbiologic assays utilizing lactobacillus fermenti and ochromonas danica were performed on 96-well microplates and growth, as monitored ... | 1992 | 1587706 |
| temperature adaptation in lactobacillus fermentum: interconversions of oleic, vaccenic and dihydrosterulic acids. | the interchange of octadecenoic acids and dihydrosterulic acid was a response of aerobically growing lactobacillus fermentum to changes in growth temperature. oleic and vaccenic acid contents decreased both at temperatures below 20 degrees c and above 26 degrees c, showing mirror image behaviour, with a concomitant increase in dihydrosterulic acid. a temperature-dependent shift from vaccenic to oleic acid synthesis, and the conversion of the latter to dihydrosterulic acid was responsible for the ... | 1992 | 1593259 |
| lactobacilli and azoreductase activity in the murine cecum. | azoreductase activity in the ceca of mice lacking lactobacilli as members of the normal microflora (reconstituted-lactobacillus-free [rlf] mice) was compared with that of rlf mice whose gastrointestinal tracts were colonized by strains of lactobacillus delbrueckii and lactobacillus fermentum. azoreductase activity was 31% lower in the ceca of mice colonized by lactobacilli. | 1991 | 1785939 |
| characteristics of lactobacilli isolated from the chicken intestine. | nine strains of lactobacilli isolated from different parts of the intestinal tract of chicken were studied to determine their taxonomy and cultural characteristics. the experimental scheme included physiological and biochemical tests, morphological observations and determination of fermentation products from glucose. all isolates were heterofermentative organisms assigned to the lactobacillus fermentum species. the relevance of intestinal lactobacilli to the nutrition of chickens was discussed. | 1991 | 1906216 |
| antibacterial activity of lactobacillus sake isolated from dry fermented sausages. | lactic acid bacteria isolated from spanish dry fermented sausages were screened for antagonistic activities under conditions that eliminated the effects of low ph and hydrogen peroxide. from 720 isolates tested 119 were inhibitory to lactobacillus fermentum cect285. the isolates showing the largest inhibitory activity exhibited an antagonistic effect against several other lactobacilli and the selected foodborne pathogens staphylococcus aureus and listeria monocytogenes. comparison of the antimic ... | 1991 | 1907472 |
| the stability of thiamine and thiamine tetrahydrofurfuryl disulfide added to table wines. | both thiamine hydrochloride and thiamine tetrahydrofurfuryl disulfide were added separately to table wines at concentrations equivalent to 0.3 and 1.5 microgram of free thiamine per kj of caloric energy. the resultant mean increments in thiamine activity, measured by lactobacillus fermenti microbiological assay after 21 months of storage, were in the range 55 to 103% of the added vitamin, indicative of high bioavailability of thiamine from this source. | 1991 | 1919806 |
| single-stranded dna plasmid, vector construction and cloning of bacillus stearothermophilus alpha-amylase in lactobacillus. | vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to taqi-digested dna of a cryptic plasmid from lactobacillus plantarum. the minimal region of lactobacillus plasmid dna that was required for dna replication was defined and a single-stranded dna intermediate replication system was observed. homologies with other origins of replication of plasmids from gram-positive bacteria, replicating via rolling circle mechanism, were found. it was shown that the c ... | 1991 | 1961976 |
| role of erythrosine in the inhibition of adhesion of lactobacillus fermentum strain 737 to mouse stomach tissue. | the mechanism by which the food colour erythrosine inhibits the adhesion of lactobacillus sp. to squamous epithelium in the mouse stomach was investigated using an in vitro adhesion assay. inhibition of adhesion occurred only after growth of l. fermentum in erythrosine which bound to the bacterial cell surface. erythrosine did not interfere with the receptor on the epithelial cell surface. growth, but not the atp content per cell, was affected by the presence of erythrosine in the growth medium. ... | 1989 | 2559943 |
| superoxide dismutase activity in some strains of lactobacilli: induction by manganese. | dialyzed cell-free extract of lactobacilli was found to contain superoxide dismutase activity by using a test system in which superoxide ion is generated by xanthine oxidase. the specific activities of lactobacillus acidophilus atcc 4356, lactobacillus murinus atcc 35020, lactobacillus acidophilus crl 358, lactobacillus plantarum atcc 8014, lactobacillus casei crl 431, lactobacillus plantarum crl 353, lactobacillus fermentum atcc 9338, lactobacillus buchneri ncdo 110, and lactobacillus fermentum ... | 1989 | 2632048 |
| comparative characterization of spirosomes isolated from lactobacillus brevis, lactobacillus fermentum, and lactobacillus buchneri. | spirosomes, cytoplasmic fine spirals, were isolated and purified from lactobacillus brevis atcc 8287, l. fermentum f-1, and l. buchneri atcc 4005, and their morphological, biochemical, and immunological properties were investigated. the spirosomes of these lactobacilli were morphologically indistinguishable from one another, and they had the same buoyant density of 1.320 g/cm3 in cscl. all of the spirosomes were composed of a single protein, spirosin, with an apparent molecular weight of about 9 ... | 1989 | 2733612 |
| adherence of trichomonas vaginalis to cell culture monolayers. | the in vitro adherence to wish cells of a pathogenic trichomonas vaginalis strain was studied with a method utilizing thymidine-labeled protozoa. a marked dose-related adherence was observed. glutaraldehyde fixed trichomonads were not adherent. the presence of fetal calf serum during the assay did not influence attachment. concanavalin a inhibited adherence of protozoa. complete or partial inhibition of adherence was achieved by preincubating wish cells with lactobacillus fermentum or streptococ ... | 1986 | 3527701 |
| protoplast fusion of lactobacillus fermentum. | tetracycline-resistant (tetr) erythromycin-resistant (eryr) fusants of lactobacillus fermentum 604 carrying a 10-megadalton tetr plasmid and l. fermentum 605 carrying a 38-megadalton eryr plasmid were obtained by means of polyethylene glycol-induced protoplast fusion. | 1986 | 3752998 |
| protective effect of lipoteichoic acid from lactobacillus casei and lactobacillus fermentum against pseudomonas aeruginosa in mice. | lipoteichoic acid (lta) from lactobacillus casei yit 9018 or lactobacillus fermentum yit 0159 augmented the resistance of c57bl/6 mice to infection with pseudomonas aeruginosa, but conferred no resistance to listeria monocytogenes. it is suggested that lta was unable to activate macrophages. | 1985 | 3934337 |
| nucleic acids of species of lactobacillus. | nucleic acid composition of 17 cultures representing the type or neotype strains of 15 named lactobacillus species was studied. nucleic acid characterization of these isolates was accompanied by a comparative study of conventional phenotypic reactions. the overall guanine plus cytosine mean deoxyribonucleic acid base composition ranged from 33 to 50% and genome sizes varied between 700 and 1500 x 10(6) daltons. sporolactobacillus inulinus contained 2500 x 10(6) daltons of chromosomal deoxyribonu ... | 1985 | 3939582 |
| growth inhibition of streptococcus mutans by cellular extracts of human intestinal lactic acid bacteria. | the in vitro growth of streptococcus mutans was completely inhibited by water-soluble extracts from cells of various intestinal lactic acid bacteria identified as streptococcus faecium, streptococcus equinus, lactobacillus fermentum, and lactobacillus salivarius. the growth inhibition was dependent on the concentrations of the extracts. in contrast, the extracts did not inhibit the growth of the major indigenous intestinal lactic acid bacteria isolated from humans. these lactic acid bacteria wer ... | 1985 | 4030098 |
| regular array in the cell wall of lactobacillus fermenti as revealed by freeze-etching and negative staining. | | 1974 | 4141740 |
| cellular location of the lipoteichoic acids of lactobacillus fermenti nctc 6991 and lactobacillus casei nctc 6375. | | 1973 | 4198220 |
| mode of action of glycine on the biosynthesis of peptidoglycan. | the mechanism of glycine action in growth inhibition was studied on eight different species of bacteria of various genera representing the four most common peptidoglycan types. to inhibit the growth of the different organisms to 80%, glycine concentrations from 0.05 to 1.33 m had to be applied. the inhibited cells showed morphological aberrations. it has been demonstrated that glycine is incorporated into the nucleotide-activated peptidoglycan precursors. the amount of incorporated glycine was e ... | 1973 | 4200845 |
| effect of ascorbic acid & thiamine on pyruvate decarboxylase activity of lactobacillus fermenti-36. | | 1967 | 4228669 |
| studies of controlled lysis of washed cell suspensions of lactobacillus fermenti and preparation of membrane-like fragments by a combined trypsin-lysozyme treatment. | | 1968 | 4238349 |
| [comparative studies using the urine metabolites of cyclophosphamide, bis-(2-chloroethyl)amine and triaziquone on lactobacillus fermenti atcc 9338]. | | 1972 | 4677072 |
| the ability to sensitize host cells for destruction by autologous complement is a general property of lipoteichoic acid. | previous studies have demonstrated that lipoteichoic acid (lta) from streptococcus pneumoniae binds to erythrocytes and renders them susceptible to lysis by autologous complement. the present study was performed to determine whether lta from two other gram-positive bacterial species had the ability to render mammalian cells susceptible to lysis by autologous complement. human erythrocytes were sensitized with lta from s. pneumoniae, streptococcus pyogenes, or lactobacillus fermentum. under incub ... | 1986 | 3533782 |
| factors affecting the resistance of lactobacillus fermenti to lysozyme. | the sensitivity of lactobacillus fermenti atcc 9338 to lysozyme has its peak during the exponential phase of growth, after the autolytic activity of the organism has begun to decline. cells from the stationary growth phase are resistant to lysozyme. the two lytic activities require different ionic conditions for their functioning; they appear mutually exclusive. incubation with trypsin renders cells from all growth phases sensitive to lysozyme. the effect of trypsin is independent of the presenc ... | 1973 | 4745431 |
| alpha-glactosidase activity of lactobacilli. | alpha-galactosidase (ec 3.2.1.22) activity was observed in cell-free extracts of lactobacillus fermenti, l. brevis, l. buchneri, l. cellobiosis, and l. salivarius subsp. salivarius. the cultural conditions under which the enzyme activity was detected suggest that the enzyme is constitutive and present in the soluble fraction in the cell. the enzyme preparations readily hydrolyzed melibiose and other oligosaccharides containing alpha(1 --> 6) linked galactose. although the cell-free extracts of l ... | 1973 | 4796954 |
| genetic transformation in lactobacillus sp. strain 100-33 of the capacity to colonize the nonsecreting gastric epithelium in mice. | lactobacillus isolates able to colonize the surfaces of the nonsecreting epithelia in the stomachs of monoassociated ex-germfree mice were derived from lactobacillus acidophilus 100-33. strain 100-33 was originally isolated from pig feces and is unable to colonize the murine gastric epithelium. in experiments involving attempts genetically to transform the capacity to colonize the epithelium, cells of strain 100-33 were treated with muralytic enzymes and mixed with polyethylene glycol and genomi ... | 1988 | 3355132 |
| purification and properties of phosphotransacetylase from lactobacillus fermenti. | | 1971 | 4995446 |
| [studies on the phosphotransacetylase of lactic acid bacteria. v. effects of some of medium constituents on the phosphotransacetylase levels of lactobacillus fermenti]. | | 1971 | 5105137 |
| studies on amino acid racemases. ii. purification and properties of the glutamate racemase from lactobacillus fermenti. | | 1969 | 5363991 |
| studies on amino acid racemases. i. partial purification and properties of the alanine racemase from lactobacillus fermenti. | | 1969 | 5367453 |
| correlation between increase in ia-bearing macrophages and induction of t cell-dependent antitumor activity by lactobacillus casei in mice. | when lactobacillus casei yit 9018 (lc 9018) or corynebacterium parvum, known to be immunomodulators possessing antitumor activity, were injected i.p. into balb/c mice, peritoneal exudate macrophage ia antigen detected by indirect immunofluorescence method was expressed on their cell surface, but it was not expressed following the injection of 10% proteose peptone, an inflammatory agent, or lactobacillus fermentum yit 0159 (lf 0159), which have no antitumor activity. the percentage and absolute n ... | 1988 | 3133111 |
| phosphotransacetylase of lactobacillus fermenti, properties of the enzyme and determination of coenzyme a. | | 1970 | 5499638 |
| isolation and properties of mutants of lactobacillus fermenti resistant to amprolium. | | 1970 | 5513334 |
| protein-mediated adhesion of lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. | the mechanism of adhesion of lactobacillus fermentum strain 737 to mouse stomach squamous epithelium was investigated. adhesion inhibition tests involving chelators, monosaccharides, periodate and concanavalin a and the use of bacteria grown in the presence of tunicamycin failed to clarify the adhesive mechanism. washed bacterial cells had reduced adhesive capacity, except in the presence of spent broth culture supernatant fraction or cell washings. spent culture supernatant fractions of erythro ... | 1989 | 2559944 |
| host-mediated antiviral activity of lactobacillus casei against cytomegalovirus infection in mice. | the protective effect of heat-killed lactobacillus casei (lc) against murine cytomegalovirus (mcmv) infection was examined. icr mice treated once with lc 1 day or 2 days before challenge survived lethal infection, but untreated or lactobacillus fermentum (lf)-treated mice did not. the protective effect was evidenced by an increase in plaque-forming units (pfu) per 50% lethal dose (ld50) and a decrease in titers of infectious viruses replicated in the target organs. this was further confirmed by ... | 1988 | 2484431 |
| protective effect of heterologous gram-positive vaccine against lethal upper respiratory tract infection with type m50 group a streptococci in mice. | type m50 group a streptococci are exceptional for their virulence in mice. however, intranasal (i.n.) vaccination with heat-killed group a streptococci, either of type m50 or m55, or an m12 strain deficient in m-protein, protected mice against i.n. challenge with m50 streptococci (82, 88 and 83% survival, respectively). significant resistance against m50 streptococci was also noted by i.n. application of heat-killed lactobacillus fermenti (81% survival) as well as two strains of pneumococci (50 ... | 1990 | 2110704 |
| characteristics of the adhesive determinants of lactobacillus fermentum 104. | the adhesion of lactobacillus fermentum 104-r and the variant strain 104-s to porcine gastric squamous epithelium was investigated. an epithelium-specific adhesion was detected for strain 104-s; however, strain 104-r expressed enhanced adhesion capacity to the control surfaces of polystyrene and bovine serum albumin. to characterize the adhesive determinants, the bacterial cells were exposed to various treatments. the adhesion pattern of bacterial cells in buffers of ph values ranging from 2 to ... | 1991 | 1849714 |
| lactobacilli on plants. | the distribution, enumeration, and identification of lactobacilli on vegetable plants were studied in an area described geographically as being subtropical and moist. the lactobacilli were obtained, by means of quantitative enrichment procedures in rogosa's sl broth, from 35.3% of all samples incubated at 32 c, and from 15.4% of the samples incubated at 45 c. less than 10 lactobacilli/g of plant material were enumerated in 54% of all positive samples. the lactobacilli were found much less freque ... | 1968 | 5676407 |
| urethanase of bacillus licheniformis sp. isolated from mouse gastrointestine. | trace levels of urethane, a cancer causing chemical, were detected in many kinds of wine, sherry, whisky, brandy and sake. urethane formation from urea and ethanol in sake can be prevented by the treatment of acid urease, which is produced by lactobacillus fermentum, but urethane, once formed, is very difficult to decompose. in order to keep the safety of alcoholic beverages, enzymatic removal of urethane has become an urgent problem. we found that bacillus licheniformis sp., isolated from mouse ... | 1991 | 1814624 |
| transport of b-vitamins in microorganisms. ii. factors affecting the uptake of labelled thiamine by nonproliferating cells of lactobacillus fermenti. | | 1966 | 5958826 |
| transport of b-vitamins in microorganisms. 3. chromatographic studies on the radioactivity extracted from non-proliferating cells of lactobacillus fermenti after exposure to labelled thiamine. | | 1966 | 5958827 |
| identification of l-ornithine and delta-aminosuccinyl ornithine in cell wall hydrolysates of lactobacillus cellobiosus. | | 1967 | 6031807 |
| isolation of an ornithine-containing cell wall precursor of lactobacillus cellobiosus. | | 1967 | 6035494 |
| properties of a lactobacillus fermenti bacteriocin. | | 1967 | 6038900 |
| detection and activity of lactacin b, a bacteriocin produced by lactobacillus acidophilus. | a total of 52 strains of lactobacillus acidophilus were examined for production of bacteriocins. a majority (63%) demonstrated inhibitory activity against all members of a four-species grouping of lactobacillus leichmannii, lactobacillus bulgaricus, lactobacillus helveticus, and lactobacillus lactis. four l. acidophilus strains with this activity also inhibited streptococcus faecalis and lactobacillus fermentum, suggesting a second system of antagonism. under conditions eliminating the effects o ... | 1983 | 6410990 |
| interaction of anti-kojibiose antibody with the lipoteichoic acids from streptococcus faecalis and streptococcus faecium. | antisera prepared in rabbits by immunization with p-aminophenyl beta-kojibioside conjugated to bovine serum albumin (antikojibiose sera), readily agglutinated whole cells of streptococcus faecalis or streptococcus faecium, and showed specific reactions with the lipoteichoic acids (ltas) of these streptococci by passive hemagglutination, microscale enzyme-linked immunosorbent assay, and crossed immunoelectrophoresis. the interaction of the antikojibiose sera with the ltas was inhibited best by ko ... | 1984 | 6434429 |
| biological utilisation of pearl millet flour fermented with yeasts and lactobacilli. | mixed culture fermentation of pearl millet flour with saccharomyces diastaticus, saccharomyces cerevisiae, lactobacillus brevis and lactobacillus fermentum brought about an improvement in its biological utilisation in rats. protein efficiency ratio, feed efficiency ratio, apparent protein digestibility, true protein digestibility, net protein utilisation, net protein retention, protein retention efficiency and utilisable protein values in case of pure culture fermented pearl millet four were hig ... | 1991 | 1796088 |
| [mobilization transfer of the pub110 plasmid between gram-positive bacteria]. | the three factor crosses between the donor strain bacillus subtilis 168 harbouring the plasmid pub102-4, bacillus thuringiensis strain carrying the mobilizing plasmid pam beta 1 and recipient strain lactobacillus fermenti were conducted in order to elaborate the optimal conditions of the plasmid pub102-4 mobilization for transfer into gram-positive microorganisms and to elucidate the possible expression of endogluconase genes in a lactobacillus strain. the lactobacillus fermenti transconjugants ... | 1991 | 1745269 |
| in vitro studies on distribution of indigenous lactobacilli of the gastrointestinal tract of rats. | to learn the biochemical mechanisms controlling the distribution of indigenous lactobacilli in the gastrointestinal tracts of rats, the effect of ph and stomach and cecal contents on lactobacillus distribution was investigated in vitro with a mixed culture of three lactobacillus strains isolated from the rat intestine. the ph of the growth medium affected the growth of lactobacilli strongly, irrespective of the lumenal contents. lactobacillus fermentum outnumbered l. acidophilus and l. murini at ... | 1980 | 6767173 |
| growth phase, cellular hydrophobicity, and adhesion in vitro of lactobacilli colonizing the keratinizing gastric epithelium in the mouse. | lactobacillus strains of numerous species isolated from several animal sources exhibited cellular hydrophobicities that differed from those expected on the basis of their abilities to colonize the keratinizing stratified squamous epithelium in the mouse stomach. cells of lactobacillus fermentum 100-33, grown to either exponential or stationary phase, were strongly hydrophilic. by contrast, cells of l. fermentum ri and six transformant derivatives of strain ri and 100-33, strains dm101 through dm ... | 1992 | 1622276 |
| development of a simple passive haemagglutination-inhibition assay for listeria monocytogenes lipoteichoic acid. | a simple assay for listeria lipoteichoic acid was devised. it is based on the property of this substance to attach spontaneously to erythrocytes. thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. cross-reactivity of lipoteichoic acid from lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal ... | 1981 | 6792287 |
| 2,4,5-trichlorophenoxyacetic acid influence on 2,6-dinitrotoluene-induced urine genotoxicity in fischer 344 rats: effect on gastrointestinal microflora and enzyme activity. | 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) and 2,6-dinitrotoluene (2,6-dnt) are hazardous chemicals that have potential harmful effects. 2,6-dnt is recognized as a hepatotoxicant while 2,4,5-t, a component of agent orange, is also suspect. 2,6-dnt requires both oxidative and reductive metabolism to elicit genotoxic effects. to determine what effect 2,4,5-t had on 2,6-dnt metabolism, intestinal enzymes, microbial populations, and urine mutagenicity were examined during 2,4,5-t treatment. weanlin ... | 1992 | 1601224 |
| adhesion to porcine squamous epithelium of saccharide and protein moieties of lactobacillus fermentum strain 104-s. | the mechanism by which lactobacillus fermentum strain 104-s adheres to porcine squamous epithelium was investigated by studying the adsorption to epithelial cells, and control surfaces, of radioactively labelled material released from the bacterial cells by water extraction. the released material was fractionated by gel filtration and the adsorption of pronase-sensitive and -resistant material in the various fractions to porcine gastric tissue and the control surfaces of polystyrene and immobili ... | 1992 | 1487731 |
| purification and characterization of acid urease from lactobacillus fermentum. | acid urease was purified to an electrophoretically homogeneous state, and the molecular weight was estimated to be 220,000. the enzyme consisted of three kinds of subunits, designated alpha, beta and gamma, with molecular weights of 67,000, 16,800 and 8600, respectively, in a (alpha 1 beta 2 gamma 1)2 structure. the isoelectric point of the enzyme was 4.8. the nickel content was found to be 1.9 atoms of nickel per alpha 1 beta 2 gamma 1 unit. the amino acid profile was different from those of kn ... | 1990 | 1366438 |
| binding of lactobacillus reuteri to fibronectin immobilized on glass beads. | human fibronectin was immobilized on glass beads. the beads were used to evaluate binding of lactobacillus reuteri to fibronectin. organisms bound to the glass beads were detected using fluorescence microscopy after treatment with acridine orange. this binding was confirmed and quantified with the use of [3h]-labelled organisms. three strains of lactobacillus reuteri, three strains of lactobacillus acidophilus and one strain of lactobacillus fermentum were tested for binding capacity. l. reuteri ... | 1992 | 1303695 |
| ultrastructure of lactobacillus fermentum during early and late growth phases and during thiamine deficiency. | thin sections of exponentially growing and stationary-phase cells of l. fermentum from thiamine sufficient and thiamine deficient media were studied by electron microscopy. compared to the exponential-phase cells the stationary-phase cells from both types of media had thicker cell walls and cross walls and fewer and smaller granules of storage material. exponential-phase, thiamine deficient cells had rather thin cell walls and small mesosomes. | 1975 | 1179752 |
| genetic analysis of acidocin b, a novel bacteriocin produced by lactobacillus acidophilus. | the genes encoding the production of acidocin b, a bacteriocin produced by lactobacillus acidophilus strain m46 which is active against listeria monocytogenes, clostridium sporogenes, brochothrix thermosphacta, lactobacillus fermentum and lactobacillus delbrueckii subsp. bulgaricus, but inactive against most other lactobacillus species, were previously localized on a 4 kb xbai-hindiii fragment of plasmid pcv461. in the present work, dna sequence analysis revealed the presence of three consecutiv ... | 1995 | 7551031 |
| effect of extracellular lactate on growth of rumen lactate producers. | the addition of na-lactate (50-150 mmol/l) to media with glucose had only marginal effect on the growth of rumen lactate-producing bacteria at ph between 6.5 and 5.8. butyrivibrio fibrisolvens was somewhat more sensitive to external lactate than streptococcus bovis, lactobacillus fermentum and selenomonas ruminantium. it can be concluded that rumen lactate producers, which proliferate at the onset of rumen lactic acidosis, are not influenced by the lactate accumulation, except some non-specific ... | 1994 | 7619002 |
| lysis of modified walls from lactobacillus fermentum. | the n and o substitution in wall peptidoglycan from lactobacillus fermentum was studied in relation to growth phase, as well as the lytic activities and the effect of trypsin on them. the n-nonsubstituted sites were determined by dinitrophenylation techniques. the results indicate that an extensive substitution at the o groups takes place as cells go into the stationary growth phase, concomitant with a decrease in their lysozyme sensitivity. n-nonsubstituted residues, mainly glucosamine, occurre ... | 1975 | 1176437 |
| [a microflora study of the gastrointestinal tract of mink housed within and outside of the area of the chernobyl atomic electric power station]. | quantitative differences in the content of lactic acid bacteria isolated from the content of the stomach, small and large intestine have been established when studying microflora of the gastrointestinal tract of minks kept in the 30-kilometer zone of the chernobyl npp (experimental animals) and at the cherkassy fur farm (control animals). obligate heterofermentative species of lactic acid bacteria related to lactobacillus fermentum and l. reuteri prevailed in the stomach of experimental minks. s ... | 1995 | 7663745 |
| pathogenic potential of lactobacilli. | lactobacilli are often considered to be commensal or beneficial participants in human microbial ecology and considerable research is being carried out into the effects of the use of lactobacilli as additives in both human and animal diets. however, lactobacilli also cause some human diseases (e.g. dental caries, rheumatic vascular disease, septicaemia and infective endocarditis (ie)), and have recently been identified as potential emerging pathogens in elderly and immunocompromised patients, par ... | 1994 | 7703012 |
| [the influence of the antivitamine pyrithiamine on the metabolism of the thiamin-autotrophic serratia marcescens]. | a new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. the method uses a mutant of lactobacillus fermenti (atcc 9338) stimulated by pyrithiamine. by this specific method it is found that serratia marcescens is able to consume and to phosphorylate the antivitamine (presumably to pyrithiamine monophosphate). the uptake of the analog influences neither the generation time of serratia marcescens nor the biomass of t ... | 1975 | 1100010 |
| genetic modification of a vaginal strain of lactobacillus fermentum and its maintenance within the reproductive tract after intravaginal administration. | many micro-organisms cause important diseases of the female genital tract. because systematic vaccination does not usually provide a good immune response at mucosal sites, commensal lactobacilli from the female genital tract were developed as vehicles to deliver continued doses of foreign antigen directly to the genital mucosal surface with the aim of stimulating strong local mucosal immune responses. lactobacilli were shown to be common inhabitants of the genital tract of the animal model studi ... | 1994 | 7932620 |
| presence of fine spirals (spirosomes) in lactobacillus fermenti and lactobacillus casei. | | 1975 | 809611 |
| [bacteriocin properties of lactobacillus fermenti, lactobacillus brevis and lactobacillus buchneri]. | four bacteriocins of l. fermenti, 3 bacteriocins of l. brevis and 1 bacteriocin of l. buchneri were studied with respect to morphology of the inhibition growth zones of the indicator strains, capacity for diffusion through cellophane, sensitivity to high temperature, bacterial proteases, trypsin, chymotrypsin, pepsin, papain, nucleases and lysozyme. according to the differences in their properties the bacteriocins were classified as belonging to 8 types, including 4 types of l. fermenti bacterio ... | 1979 | 507777 |
| the aggregation of human platelets by lactobacillus species. | the ability to aggregate human platelets was examined for five lactobacillus rhamnosus strains and five lactobacillus paracasei subsp. paracasei strains isolated from patients with infective endocarditis (ie), 25 laboratory isolates from the same two species, and 14 strains from five other oral species, namely lactobacillus acidophilus, lactobacillus fermentum, lactobacillus oris, lactobacillus plantarum and lactobacillus salivarius. amongst the l. rhamnosus strains, platelets were aggregated by ... | 1993 | 8126421 |
| antigenic analyses of lactobacillus fermenti. | antigenic analyses of lactobacillus fermenti were carried out by double immunodiffusion in agar using extracts prepared with cold trichloroacetic acid (tca) or hot dilute hydrochloric acid (hcl). a common antigen of l. fermenti, designated as antigen f by the author, was extracted from whole cells with dilute hcl, but not with tca. the antigen f was also observed in lactobacillus casei. in addition, all strains isolated from human saliva contained antigen 6 in their cell walls, while the antigen ... | 1975 | 50468 |
| extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilue acid. | autoclaving cell wall of streptococcus mutans ingbritt for 15 min under the rantz and randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. the extract contained the serotype c-specific antigen but lacked the lipoteichoic acid component extracted when whole cells were autoclaved. autoclaving cell wall preparations from other strains of s. mutans and also streptococcus salivarius and streptococcus mitis in 0.85% ... | 1978 | 32140 |
| changes in the microflora and physiology of the anterior intestinal tract of pigs weaned at 2 days, with special reference to the pathogenesis of diarrhea. | the gastrointestinal microflora and gastric physiology of piglets weaned at 2 days was compared with that of piglets allowed to continue sucking the sow. although there was a significantly higher count of escherichia coli in the stomach, duodenum, and jejunum of the early-weaned compared with sow-reared pigs, these differences were not detectable in samples from the ileum. there were no quantitative differences in lactobacilli and in streptococci between the two treatments. lactobacillus ferment ... | 1977 | 22488 |
| coaggregation of oral lactobacilli with streptococci from the oral cavity. | the ability of oral lactobacilli to coaggregate with streptococci and actinomycetes was investigated. of the 7 species of lactobacilli studied, only two were capable of coaggregation and the coaggregation was restricted to streptococci. lactobacillus salivarius strains (2/4) coaggregated with streptococcus salivarius, streptococcus gordonii, streptococcus crista and tufted streptococcus sanguis ii strains. lactobacillus fermentum (2/3) coaggregated with s. gordonii and s. sanguis. the coaggregat ... | 1993 | 8265207 |
| [studies on the locality and the function of spirosome in bacterial cells: relationship between the production of spirosome and anaerobic glycolysis]. | the locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and electron microscopy in peptostreptococcus productus, clostridium sp. hd-17, lactobacillus fermentum, eubacterium aerofaciens and escherichia cali b. when the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of ... | 1993 | 8315808 |
| comparative studies on the isolation of membrane lipoteichoic acid from lactobacillus fermenti. | preparations of membrane lipoteichoic acid containing different amounts of protein were isolated from intact organisms of lactobacillus fermenti nctc 6991 by various procedures chosen for their ability to disrupt the hydrophobic interaction of lipoteichoic acid with other membrane components. the highest yield of lipoteichoic acid was obtained with hot aqueous phenol, and this preparation contained very little protein. partial removal of cell lipids with chloroform-methanol followed by extractio ... | 1973 | 4734578 |
| autolytic enzyme system from lactobacillus fermenti. | | 1973 | 4711466 |
| studies of thiamine uptake in growing cultures and in cell fragments of lactobacillus fermenti. | | 1971 | 4947426 |
| the antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation. | a total of 241 lactic acid bacteria belonging to lactobacillus plantarum, pediococcus pentosaceus, lactobacillus fermentum/reuteri and lactobacillus brevis from various processing stages of maize dough fermentation were investigated. results indicated that each processing stage has its own microenvironment with strong antimicrobial activity. about half of the lact. plantarum and practically all of the lact. fermentum/reuteri investigated were shown to inhibit other gram-positive and gram-negativ ... | 1995 | 8567490 |
| studies on the group f antigen of lactobacilli: isolation of a teichoic acid-lipid complex from lactobacillus fermenti nctc 6991. | | 1970 | 5487616 |
| the potential of lactobacillus as a carrier for oral immunization: development and preliminary characterization of vector systems for targeted delivery of antigens. | oral administration of lactobacilli evokes mucosal and systemic immune responses against epitopes associated with these organisms (gerritse et al., 1990, 1991). the adjuvant function of different lactobacillus species was investigated under the conditions of intraperitoneal (i.p.) injection or oral administration. after i.p. injection of trinitrophenylated chicken gamma-globulin, high dth responses were observed with lactobacillus casei and lactobacillus plantarum, but low responses with lactoba ... | 1996 | 8717402 |
| effect of adherent lactobacillus spp. on in vitro adherence of salmonellae to the intestinal epithelial cells of chicken. | single strains of lactobacillus acidophilus and lact. fermentum, isolated from chicken intestine, were used to study in vitro interactions with salmonella enteritidis, salm. pullorum or salm. typhimurium in an ileal epithelial cell (iec) radioactive assay. exclusion, competition and displacement phenomena were investigated by respectively incubating (a) lactobacilli and iec together, prior to addition of salmonellae, (b) lactobacilli, iec and salmonellae together, and (c) salmonellae and iec, fo ... | 1996 | 8760330 |
| inhibition of initial adhesion of uropathogenic enterococcus faecalis by biosurfactants from lactobacillus isolates. | in this study, 15 lactobacillus isolates were found to produce biosurfactants in the mid-exponential and stationary growth phases. the stationary-phase biosurfactants from lactobacillus casei subsp. rhamnosus 36 and atcc 7469, lactobacillus fermentum b54, and lactobacillus acidophilus rc14 were investigated further to determine their capacity to inhibit the initial adhesion of uropathogenic enterococcus faecalis 1131 to glass in a parallel-plate flow chamber. the initial deposition rate of e. fa ... | 1996 | 8787394 |
| [studies on the phosphotransacetylase of lactic acid bacteria. ii. enzymatic preparation of acetyl-coenzyme a and propionyl-coenzyme a using phosphotransacetylase from lactobacillus fermenti]. | | 1970 | 5530456 |
| purification and characterization of a component produced by lactobacillus fermentum that inhibits the adhesion of k88 expressing escherichia coli to porcine ileal mucus. | it has previously been shown that lactobacillus fermentum strain 104r releases compounds into its culture fluid that inhibit the adhesion of enterotoxigenic escherichia coli k88. the aim of the present study was to purify and identify this compound. judged by gel filtration, the compound was found to be approximately 1700 kda. the amount of active compound increased upon prolonged incubation, while the number of viable cells reduced, suggesting that the activity was coming from dead cells. as th ... | 1996 | 8852678 |
| influence of carbohydrates on the alpha-galactosidase activity of lactobacillus fermentum | the influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from lactobacillus fermentum crl 251 was studied. stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; scarce activity was detected with glucose. exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of l. fermentum growing on galactose. this effect was reverse ... | 1996 | 8875910 |
| a bifunctional urease enhances survival of pathogenic yersinia enterocolitica and morganella morganii at low ph. | to infect a susceptible host, the gastrointestinal pathogen yersinia enterocolitica must survive passage through the acid environment of the stomach. in this study, we showed that y. enterocolitica serotype o8 survives buffered acidic conditions as low as ph 1.5 for long periods of time provided urea is available. acid tolerance required an unusual cytoplasmically located urease that was activated 780-fold by low-ph conditions. acid tolerance of helicobacter species has also been attributed to u ... | 1996 | 8932305 |
| cell wall and peptidoglycan from lactobacillus fermenti. | cell walls from lactobacillus fermenti were prepared by differential centrifugation of disrupted cells, with and without trypsin treatment. approximately 16% of the dry weight of walls was found in a crude trichloroacetic acid extract of the walls; half of this amount remained upon further purification. the purufied extract lacked alanine, but contained substantial amounts of glucosamine. the walls constituted 23 to 33% of the dry weight of the cell. the chemical composition of the various types ... | 1971 | 5547995 |
| growth promoting effect of oxythiamine on lactobacillus fermenti. | | 1967 | 5601992 |
| nucleotide sequence of the bacillus licheniformis atcc 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species. | the gene encoding the d-aminotransferase from bacillus licheniformis was cloned and the complete dna sequence was determined. the deduced d-aminotransferase protein sequence, consists of 283 amino acids and shows a high degree of homology with other bacillus d-aminotransferases, branched chain aminotransferase of escherichia coli and the 4-amino-benzoate-4-deoxychorismate lyase of bacillus subtilis and escherichia coli. | 1997 | 9003455 |
| [the antagonistic properties of microaerophilic bacteria isolated from the human and mink digestive tracts]. | study of antagonistic properties of microaerophilic bacteria isolated from human and mink gastroenteric tract have helped to establish differences in species composition, quantity and level of antagonistic activity of the studied microorganisms in respect to pathogenic microflora. it is shown that lactic acid bacteria identified as lactobacillus fermentum and l. reuteri prevail among the strains isolated from the stomach and thin intestine of minks kept in the 30-km zone of chernobyl npp. specie ... | 1996 | 9044713 |
| structure of the cell wall of lactobacilli. role of muramic acid phosphate in lactobacillus fermenti. | 1. the polysaccharide and mucopeptide components of the cell wall of lactobacillus fermenti, serological group f, were separated by mild conditions of acid hydrolysis; the polysaccharide was composed of glucose and galactose. 2. soluble cell-wall products were isolated from cell wall lysed by lysozyme and a streptomyces enzyme preparation. the lysozyme-dissolved fraction contained a greater proportion of mucopeptide. 3. the soluble preparations were heated in dilute acid to hydrolyse the linkage ... | 1968 | 5667252 |
| use of the stereoisomers of isoleucine by lactobacillus fermenti. | the use of four stereoisomers of isoleucine by lactobacillus fermenti strain 36 was studied in detail. all four isoleucine isomers were used for growth in the presence of vitamin b(6) compounds, but only l-isoleucine was active in the absence of these vitamins. of the vitamin b(6) compounds, pyridoxal and pyridoxamine were equally more effective than pyridoxine for the utilization of these isomers. lowering the initial ph, decreasing the amounts of leucine and valine, and adapting the organism t ... | 1965 | 5867648 |
| cell wall anchoring of the streptococcus pyogenes m6 protein in various lactic acid bacteria. | the m6 protein from streptococcus pyogenes is the best-characterized member of a family of cell envelope-associated proteins. based on the observation that the c-terminal sorting signals of these proteins can drive cell wall anchoring of heterologous unanchored proteins, we have cloned and expressed the emm6 structural gene for the m6 protein in various lactic acid bacteria (lab). the emm6 gene was successfully expressed from lactococcal promoters in several lactococcus lactis strains, an animal ... | 1997 | 9139932 |