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changes in the microflora and physiology of the anterior intestinal tract of pigs weaned at 2 days, with special reference to the pathogenesis of diarrhea.the gastrointestinal microflora and gastric physiology of piglets weaned at 2 days was compared with that of piglets allowed to continue sucking the sow. although there was a significantly higher count of escherichia coli in the stomach, duodenum, and jejunum of the early-weaned compared with sow-reared pigs, these differences were not detectable in samples from the ileum. there were no quantitative differences in lactobacilli and in streptococci between the two treatments. lactobacillus ferment ...197722488
extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilue acid.autoclaving cell wall of streptococcus mutans ingbritt for 15 min under the rantz and randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. the extract contained the serotype c-specific antigen but lacked the lipoteichoic acid component extracted when whole cells were autoclaved. autoclaving cell wall preparations from other strains of s. mutans and also streptococcus salivarius and streptococcus mitis in 0.85% ...197832140
urease-producing species of intestinal anaerobes and their activities.urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ...197936839
antigenic analyses of lactobacillus fermenti.antigenic analyses of lactobacillus fermenti were carried out by double immunodiffusion in agar using extracts prepared with cold trichloroacetic acid (tca) or hot dilute hydrochloric acid (hcl). a common antigen of l. fermenti, designated as antigen f by the author, was extracted from whole cells with dilute hcl, but not with tca. the antigen f was also observed in lactobacillus casei. in addition, all strains isolated from human saliva contained antigen 6 in their cell walls, while the antigen ...197550468
[role of normal microflora in the treatment of vibrio carrier state in rat gnotobionts].materials on the study of interactions between vibrio cholera el tor and some indigenous bacteria of human intestinal tract, which proved to be active antagonists in vitro, are presented in this work. experiments were conducted on gnotobiotic rats (fisher), using various combinations and variants of the order of the microorganism administration; there were revealed no antagonistic relations between virbrio cholerae el tor and escherichia coli m-17, lactobacillus fermenti, lactobacillus plantarum ...1978352437
serological studies on chemostat-grown cultures of lactobacillus fermentum and lactobacillus plantarum.lactobacillus fermentum nctc 6991 and lactobacillus plantarum ncib 7220 were grown in a chemostat in the diffusible fraction of complex medium at ph 6.0 with glucose limitation. organisms grown at different dilution rates (d) were injected into rabbits, and the resultant antisera were examined for reactivity with antigens previously isolated from batch-grown organisms. for l. fermentum, antisera obtained on injecting cells grown at d = 0.5 h-1 contained a significantly higher level of antibody r ...1979457268
[bacteriocin properties of lactobacillus fermenti, lactobacillus brevis and lactobacillus buchneri].four bacteriocins of l. fermenti, 3 bacteriocins of l. brevis and 1 bacteriocin of l. buchneri were studied with respect to morphology of the inhibition growth zones of the indicator strains, capacity for diffusion through cellophane, sensitivity to high temperature, bacterial proteases, trypsin, chymotrypsin, pepsin, papain, nucleases and lysozyme. according to the differences in their properties the bacteriocins were classified as belonging to 8 types, including 4 types of l. fermenti bacterio ...1979507777
[influence of some thiamine antagonists on the growth of kloeckera apiculata, lactobacillus fermenti and lactobacillus arabinosus (author's transl)]. 1977557103
bacteria associated with the gastric epithelium of neonatal pigs.light and electron microscopy showed lactobacilli and, to a lesser degree, streptococci to be closely associated with the squamous area of the pig stomach known as the pars esophagea. several different types of extracellular layers were seen on bacteria attached to the epithelial surface. the total number of bacteria per square centimeter did not change with age up to 10 days, and there was no effect of weaning at 2 days. lactobacillus fermentum, l. salivarius, and streptococcus salivarius were ...1978637551
presence of fine spirals (spirosomes) in lactobacillus fermenti and lactobacillus casei. 1975809611
origin of esterases in human whole saliva.whole saliva of 59 healthy persons was used for determination of esterase activity. the pattern of esterase was studied by means of isoelectrofocusing on thin-layer acrylamide gels. the esterases found in whole saliva are suggested to be derived from the cells of the tissue in the oral cavity. this origin is indicated (e.g.) by comparison between isolectrophoretic esterase patterns of whole saliva, submandibular saliva, gingival biopsy and fibroblast culture. antisera against partially purified ...1977862601
penicillin resistance and its elimination by treatment with acriflavine in lactobacillus fermenti. 1976966394
[the influence of the antivitamine pyrithiamine on the metabolism of the thiamin-autotrophic serratia marcescens].a new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. the method uses a mutant of lactobacillus fermenti (atcc 9338) stimulated by pyrithiamine. by this specific method it is found that serratia marcescens is able to consume and to phosphorylate the antivitamine (presumably to pyrithiamine monophosphate). the uptake of the analog influences neither the generation time of serratia marcescens nor the biomass of t ...19751100010
characterization of group n streptococcus lipoteichoic acid.lipoteichoic acid was extracted from the group n organism streptococcus lactis atcc 9936 with hot aqueous phenol and purified by gel chromatography followed by affinity chromatography using ricinus communis lectin as the specific absorbent. the teichoic acid moiety of the lipoteichoic acid was calculated to contain 16 to 17 glycerol phosphate units, approximately half of which were substituted with alpha-d-galactosyl residues; the glycolipid moiety contained o-alpha-d-glucosyl-1 yields 2-o-alpha ...19751123256
formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli.examination of the culture fluids from a number of strains of oral streptococci and latobacilli has shown the presence of an erythrocyte-sensitizing antigen with the properties of lipoteichoic acid. the antigen was isolated from the culture fluids of lactobacillus casei and lactobacillus fermentum and characterized chemically and serologically, for other strains, serological evidence for the presence of lipoteichoic acid depends on the reactivity with antiserum specific for the glycerol phosphat ...19751158524
lysis of modified walls from lactobacillus fermentum.the n and o substitution in wall peptidoglycan from lactobacillus fermentum was studied in relation to growth phase, as well as the lytic activities and the effect of trypsin on them. the n-nonsubstituted sites were determined by dinitrophenylation techniques. the results indicate that an extensive substitution at the o groups takes place as cells go into the stationary growth phase, concomitant with a decrease in their lysozyme sensitivity. n-nonsubstituted residues, mainly glucosamine, occurre ...19751176437
ultrastructure of lactobacillus fermentum during early and late growth phases and during thiamine deficiency.thin sections of exponentially growing and stationary-phase cells of l. fermentum from thiamine sufficient and thiamine deficient media were studied by electron microscopy. compared to the exponential-phase cells the stationary-phase cells from both types of media had thicker cell walls and cross walls and fewer and smaller granules of storage material. exponential-phase, thiamine deficient cells had rather thin cell walls and small mesosomes.19751179752
binding of lactobacillus reuteri to fibronectin immobilized on glass beads.human fibronectin was immobilized on glass beads. the beads were used to evaluate binding of lactobacillus reuteri to fibronectin. organisms bound to the glass beads were detected using fluorescence microscopy after treatment with acridine orange. this binding was confirmed and quantified with the use of [3h]-labelled organisms. three strains of lactobacillus reuteri, three strains of lactobacillus acidophilus and one strain of lactobacillus fermentum were tested for binding capacity. l. reuteri ...19921303695
purification and characterization of acid urease from lactobacillus fermentum.acid urease was purified to an electrophoretically homogeneous state, and the molecular weight was estimated to be 220,000. the enzyme consisted of three kinds of subunits, designated alpha, beta and gamma, with molecular weights of 67,000, 16,800 and 8600, respectively, in a (alpha 1 beta 2 gamma 1)2 structure. the isoelectric point of the enzyme was 4.8. the nickel content was found to be 1.9 atoms of nickel per alpha 1 beta 2 gamma 1 unit. the amino acid profile was different from those of kn ...19901366438
adhesion to porcine squamous epithelium of saccharide and protein moieties of lactobacillus fermentum strain 104-s.the mechanism by which lactobacillus fermentum strain 104-s adheres to porcine squamous epithelium was investigated by studying the adsorption to epithelial cells, and control surfaces, of radioactively labelled material released from the bacterial cells by water extraction. the released material was fractionated by gel filtration and the adsorption of pronase-sensitive and -resistant material in the various fractions to porcine gastric tissue and the control surfaces of polystyrene and immobili ...19921487731
a note on the use of a plasmid as a dna probe in the detection of a lactobacillus fermentum strain in porcine stomach contents.a plasmid (about 50 kb) was used as a dna probe to enumerate, by colony hybridization, a strain of lactobacillus fermentum in the stomach contents of eight piglets. the population sizes obtained by colony hybridization were in agreement with estimated levels calculated on the basis of plasmid profiling of colonies isolated at random from the total lactobacillus population.19921512179
existence of phosphoenolpyruvate: carbohydrate phosphotransferase systems in lactobacillus fermentum, an obligate heterofermenter.the presence or absence of the phosphoenolpyruvate: carbohydrate phosphotransferase system (pts) in obligately heterofermentative group iii lactobacilli including lactobacillus brevis (3 strains), l. buchneri (2 strains) and l. fermentum (3 strains) was surveyed systematically for a series of sugars utilizable by these organisms. contrary to common expectation, ptss were found in two strains of l. fermentum: sucrose-pts in one strain; sucrose- and mannose-ptss in the other. all these activities ...19921513267
microbiological methods of thiamine measurement in biological material.semiautomated, microbiological methods for the determination of thiamine in a variety of biological material are presented. thiamine content was measured in bovine, ovine and rat blood, plasma, bile, urine, duodenal fluid, liver, kidney and brain. thiamine was extracted from samples by mild acid digestion (trans aconitate ph 4.0) at 115 degrees c for 20 min. microbiologic assays utilizing lactobacillus fermenti and ochromonas danica were performed on 96-well microplates and growth, as monitored ...19921587706
temperature adaptation in lactobacillus fermentum: interconversions of oleic, vaccenic and dihydrosterulic acids.the interchange of octadecenoic acids and dihydrosterulic acid was a response of aerobically growing lactobacillus fermentum to changes in growth temperature. oleic and vaccenic acid contents decreased both at temperatures below 20 degrees c and above 26 degrees c, showing mirror image behaviour, with a concomitant increase in dihydrosterulic acid. a temperature-dependent shift from vaccenic to oleic acid synthesis, and the conversion of the latter to dihydrosterulic acid was responsible for the ...19921593259
2,4,5-trichlorophenoxyacetic acid influence on 2,6-dinitrotoluene-induced urine genotoxicity in fischer 344 rats: effect on gastrointestinal microflora and enzyme activity.2,4,5-trichlorophenoxyacetic acid (2,4,5-t) and 2,6-dinitrotoluene (2,6-dnt) are hazardous chemicals that have potential harmful effects. 2,6-dnt is recognized as a hepatotoxicant while 2,4,5-t, a component of agent orange, is also suspect. 2,6-dnt requires both oxidative and reductive metabolism to elicit genotoxic effects. to determine what effect 2,4,5-t had on 2,6-dnt metabolism, intestinal enzymes, microbial populations, and urine mutagenicity were examined during 2,4,5-t treatment. weanlin ...19921601224
growth phase, cellular hydrophobicity, and adhesion in vitro of lactobacilli colonizing the keratinizing gastric epithelium in the mouse.lactobacillus strains of numerous species isolated from several animal sources exhibited cellular hydrophobicities that differed from those expected on the basis of their abilities to colonize the keratinizing stratified squamous epithelium in the mouse stomach. cells of lactobacillus fermentum 100-33, grown to either exponential or stationary phase, were strongly hydrophilic. by contrast, cells of l. fermentum ri and six transformant derivatives of strain ri and 100-33, strains dm101 through dm ...19921622276
[mobilization transfer of the pub110 plasmid between gram-positive bacteria].the three factor crosses between the donor strain bacillus subtilis 168 harbouring the plasmid pub102-4, bacillus thuringiensis strain carrying the mobilizing plasmid pam beta 1 and recipient strain lactobacillus fermenti were conducted in order to elaborate the optimal conditions of the plasmid pub102-4 mobilization for transfer into gram-positive microorganisms and to elucidate the possible expression of endogluconase genes in a lactobacillus strain. the lactobacillus fermenti transconjugants ...19911745269
lactobacilli and azoreductase activity in the murine cecum.azoreductase activity in the ceca of mice lacking lactobacilli as members of the normal microflora (reconstituted-lactobacillus-free [rlf] mice) was compared with that of rlf mice whose gastrointestinal tracts were colonized by strains of lactobacillus delbrueckii and lactobacillus fermentum. azoreductase activity was 31% lower in the ceca of mice colonized by lactobacilli.19911785939
biological utilisation of pearl millet flour fermented with yeasts and lactobacilli.mixed culture fermentation of pearl millet flour with saccharomyces diastaticus, saccharomyces cerevisiae, lactobacillus brevis and lactobacillus fermentum brought about an improvement in its biological utilisation in rats. protein efficiency ratio, feed efficiency ratio, apparent protein digestibility, true protein digestibility, net protein utilisation, net protein retention, protein retention efficiency and utilisable protein values in case of pure culture fermented pearl millet four were hig ...19911796088
urethanase of bacillus licheniformis sp. isolated from mouse gastrointestine.trace levels of urethane, a cancer causing chemical, were detected in many kinds of wine, sherry, whisky, brandy and sake. urethane formation from urea and ethanol in sake can be prevented by the treatment of acid urease, which is produced by lactobacillus fermentum, but urethane, once formed, is very difficult to decompose. in order to keep the safety of alcoholic beverages, enzymatic removal of urethane has become an urgent problem. we found that bacillus licheniformis sp., isolated from mouse ...19911814624
characteristics of the adhesive determinants of lactobacillus fermentum 104.the adhesion of lactobacillus fermentum 104-r and the variant strain 104-s to porcine gastric squamous epithelium was investigated. an epithelium-specific adhesion was detected for strain 104-s; however, strain 104-r expressed enhanced adhesion capacity to the control surfaces of polystyrene and bovine serum albumin. to characterize the adhesive determinants, the bacterial cells were exposed to various treatments. the adhesion pattern of bacterial cells in buffers of ph values ranging from 2 to ...19911849714
characteristics of lactobacilli isolated from the chicken intestine.nine strains of lactobacilli isolated from different parts of the intestinal tract of chicken were studied to determine their taxonomy and cultural characteristics. the experimental scheme included physiological and biochemical tests, morphological observations and determination of fermentation products from glucose. all isolates were heterofermentative organisms assigned to the lactobacillus fermentum species. the relevance of intestinal lactobacilli to the nutrition of chickens was discussed.19911906216
antibacterial activity of lactobacillus sake isolated from dry fermented sausages.lactic acid bacteria isolated from spanish dry fermented sausages were screened for antagonistic activities under conditions that eliminated the effects of low ph and hydrogen peroxide. from 720 isolates tested 119 were inhibitory to lactobacillus fermentum cect285. the isolates showing the largest inhibitory activity exhibited an antagonistic effect against several other lactobacilli and the selected foodborne pathogens staphylococcus aureus and listeria monocytogenes. comparison of the antimic ...19911907472
the stability of thiamine and thiamine tetrahydrofurfuryl disulfide added to table wines.both thiamine hydrochloride and thiamine tetrahydrofurfuryl disulfide were added separately to table wines at concentrations equivalent to 0.3 and 1.5 microgram of free thiamine per kj of caloric energy. the resultant mean increments in thiamine activity, measured by lactobacillus fermenti microbiological assay after 21 months of storage, were in the range 55 to 103% of the added vitamin, indicative of high bioavailability of thiamine from this source.19911919806
single-stranded dna plasmid, vector construction and cloning of bacillus stearothermophilus alpha-amylase in lactobacillus.vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to taqi-digested dna of a cryptic plasmid from lactobacillus plantarum. the minimal region of lactobacillus plasmid dna that was required for dna replication was defined and a single-stranded dna intermediate replication system was observed. homologies with other origins of replication of plasmids from gram-positive bacteria, replicating via rolling circle mechanism, were found. it was shown that the c ...19911961976
protective effect of heterologous gram-positive vaccine against lethal upper respiratory tract infection with type m50 group a streptococci in mice.type m50 group a streptococci are exceptional for their virulence in mice. however, intranasal (i.n.) vaccination with heat-killed group a streptococci, either of type m50 or m55, or an m12 strain deficient in m-protein, protected mice against i.n. challenge with m50 streptococci (82, 88 and 83% survival, respectively). significant resistance against m50 streptococci was also noted by i.n. application of heat-killed lactobacillus fermenti (81% survival) as well as two strains of pneumococci (50 ...19902110704
host-mediated antiviral activity of lactobacillus casei against cytomegalovirus infection in mice.the protective effect of heat-killed lactobacillus casei (lc) against murine cytomegalovirus (mcmv) infection was examined. icr mice treated once with lc 1 day or 2 days before challenge survived lethal infection, but untreated or lactobacillus fermentum (lf)-treated mice did not. the protective effect was evidenced by an increase in plaque-forming units (pfu) per 50% lethal dose (ld50) and a decrease in titers of infectious viruses replicated in the target organs. this was further confirmed by ...19882484431
role of erythrosine in the inhibition of adhesion of lactobacillus fermentum strain 737 to mouse stomach tissue.the mechanism by which the food colour erythrosine inhibits the adhesion of lactobacillus sp. to squamous epithelium in the mouse stomach was investigated using an in vitro adhesion assay. inhibition of adhesion occurred only after growth of l. fermentum in erythrosine which bound to the bacterial cell surface. erythrosine did not interfere with the receptor on the epithelial cell surface. growth, but not the atp content per cell, was affected by the presence of erythrosine in the growth medium. ...19892559943
protein-mediated adhesion of lactobacillus fermentum strain 737 to mouse stomach squamous epithelium.the mechanism of adhesion of lactobacillus fermentum strain 737 to mouse stomach squamous epithelium was investigated. adhesion inhibition tests involving chelators, monosaccharides, periodate and concanavalin a and the use of bacteria grown in the presence of tunicamycin failed to clarify the adhesive mechanism. washed bacterial cells had reduced adhesive capacity, except in the presence of spent broth culture supernatant fraction or cell washings. spent culture supernatant fractions of erythro ...19892559944
superoxide dismutase activity in some strains of lactobacilli: induction by manganese.dialyzed cell-free extract of lactobacilli was found to contain superoxide dismutase activity by using a test system in which superoxide ion is generated by xanthine oxidase. the specific activities of lactobacillus acidophilus atcc 4356, lactobacillus murinus atcc 35020, lactobacillus acidophilus crl 358, lactobacillus plantarum atcc 8014, lactobacillus casei crl 431, lactobacillus plantarum crl 353, lactobacillus fermentum atcc 9338, lactobacillus buchneri ncdo 110, and lactobacillus fermentum ...19892632048
comparative characterization of spirosomes isolated from lactobacillus brevis, lactobacillus fermentum, and lactobacillus buchneri.spirosomes, cytoplasmic fine spirals, were isolated and purified from lactobacillus brevis atcc 8287, l. fermentum f-1, and l. buchneri atcc 4005, and their morphological, biochemical, and immunological properties were investigated. the spirosomes of these lactobacilli were morphologically indistinguishable from one another, and they had the same buoyant density of 1.320 g/cm3 in cscl. all of the spirosomes were composed of a single protein, spirosin, with an apparent molecular weight of about 9 ...19892733612
correlation between increase in ia-bearing macrophages and induction of t cell-dependent antitumor activity by lactobacillus casei in mice.when lactobacillus casei yit 9018 (lc 9018) or corynebacterium parvum, known to be immunomodulators possessing antitumor activity, were injected i.p. into balb/c mice, peritoneal exudate macrophage ia antigen detected by indirect immunofluorescence method was expressed on their cell surface, but it was not expressed following the injection of 10% proteose peptone, an inflammatory agent, or lactobacillus fermentum yit 0159 (lf 0159), which have no antitumor activity. the percentage and absolute n ...19883133111
genetic transformation in lactobacillus sp. strain 100-33 of the capacity to colonize the nonsecreting gastric epithelium in mice.lactobacillus isolates able to colonize the surfaces of the nonsecreting epithelia in the stomachs of monoassociated ex-germfree mice were derived from lactobacillus acidophilus 100-33. strain 100-33 was originally isolated from pig feces and is unable to colonize the murine gastric epithelium. in experiments involving attempts genetically to transform the capacity to colonize the epithelium, cells of strain 100-33 were treated with muralytic enzymes and mixed with polyethylene glycol and genomi ...19883355132
adherence of trichomonas vaginalis to cell culture monolayers.the in vitro adherence to wish cells of a pathogenic trichomonas vaginalis strain was studied with a method utilizing thymidine-labeled protozoa. a marked dose-related adherence was observed. glutaraldehyde fixed trichomonads were not adherent. the presence of fetal calf serum during the assay did not influence attachment. concanavalin a inhibited adherence of protozoa. complete or partial inhibition of adherence was achieved by preincubating wish cells with lactobacillus fermentum or streptococ ...19863527701
the ability to sensitize host cells for destruction by autologous complement is a general property of lipoteichoic acid.previous studies have demonstrated that lipoteichoic acid (lta) from streptococcus pneumoniae binds to erythrocytes and renders them susceptible to lysis by autologous complement. the present study was performed to determine whether lta from two other gram-positive bacterial species had the ability to render mammalian cells susceptible to lysis by autologous complement. human erythrocytes were sensitized with lta from s. pneumoniae, streptococcus pyogenes, or lactobacillus fermentum. under incub ...19863533782
protoplast fusion of lactobacillus fermentum.tetracycline-resistant (tetr) erythromycin-resistant (eryr) fusants of lactobacillus fermentum 604 carrying a 10-megadalton tetr plasmid and l. fermentum 605 carrying a 38-megadalton eryr plasmid were obtained by means of polyethylene glycol-induced protoplast fusion.19863752998
protective effect of lipoteichoic acid from lactobacillus casei and lactobacillus fermentum against pseudomonas aeruginosa in mice.lipoteichoic acid (lta) from lactobacillus casei yit 9018 or lactobacillus fermentum yit 0159 augmented the resistance of c57bl/6 mice to infection with pseudomonas aeruginosa, but conferred no resistance to listeria monocytogenes. it is suggested that lta was unable to activate macrophages.19853934337
nucleic acids of species of lactobacillus.nucleic acid composition of 17 cultures representing the type or neotype strains of 15 named lactobacillus species was studied. nucleic acid characterization of these isolates was accompanied by a comparative study of conventional phenotypic reactions. the overall guanine plus cytosine mean deoxyribonucleic acid base composition ranged from 33 to 50% and genome sizes varied between 700 and 1500 x 10(6) daltons. sporolactobacillus inulinus contained 2500 x 10(6) daltons of chromosomal deoxyribonu ...19853939582
growth inhibition of streptococcus mutans by cellular extracts of human intestinal lactic acid bacteria.the in vitro growth of streptococcus mutans was completely inhibited by water-soluble extracts from cells of various intestinal lactic acid bacteria identified as streptococcus faecium, streptococcus equinus, lactobacillus fermentum, and lactobacillus salivarius. the growth inhibition was dependent on the concentrations of the extracts. in contrast, the extracts did not inhibit the growth of the major indigenous intestinal lactic acid bacteria isolated from humans. these lactic acid bacteria wer ...19854030098
regular array in the cell wall of lactobacillus fermenti as revealed by freeze-etching and negative staining. 19744141740
cellular location of the lipoteichoic acids of lactobacillus fermenti nctc 6991 and lactobacillus casei nctc 6375. 19734198220
mode of action of glycine on the biosynthesis of peptidoglycan.the mechanism of glycine action in growth inhibition was studied on eight different species of bacteria of various genera representing the four most common peptidoglycan types. to inhibit the growth of the different organisms to 80%, glycine concentrations from 0.05 to 1.33 m had to be applied. the inhibited cells showed morphological aberrations. it has been demonstrated that glycine is incorporated into the nucleotide-activated peptidoglycan precursors. the amount of incorporated glycine was e ...19734200845
effect of ascorbic acid & thiamine on pyruvate decarboxylase activity of lactobacillus fermenti-36. 19674228669
studies of controlled lysis of washed cell suspensions of lactobacillus fermenti and preparation of membrane-like fragments by a combined trypsin-lysozyme treatment. 19684238349
[comparative studies using the urine metabolites of cyclophosphamide, bis-(2-chloroethyl)amine and triaziquone on lactobacillus fermenti atcc 9338]. 19724677072
autolytic enzyme system from lactobacillus fermenti. 19734711466
comparative studies on the isolation of membrane lipoteichoic acid from lactobacillus fermenti.preparations of membrane lipoteichoic acid containing different amounts of protein were isolated from intact organisms of lactobacillus fermenti nctc 6991 by various procedures chosen for their ability to disrupt the hydrophobic interaction of lipoteichoic acid with other membrane components. the highest yield of lipoteichoic acid was obtained with hot aqueous phenol, and this preparation contained very little protein. partial removal of cell lipids with chloroform-methanol followed by extractio ...19734734578
factors affecting the resistance of lactobacillus fermenti to lysozyme.the sensitivity of lactobacillus fermenti atcc 9338 to lysozyme has its peak during the exponential phase of growth, after the autolytic activity of the organism has begun to decline. cells from the stationary growth phase are resistant to lysozyme. the two lytic activities require different ionic conditions for their functioning; they appear mutually exclusive. incubation with trypsin renders cells from all growth phases sensitive to lysozyme. the effect of trypsin is independent of the presenc ...19734745431
alpha-glactosidase activity of lactobacilli.alpha-galactosidase (ec 3.2.1.22) activity was observed in cell-free extracts of lactobacillus fermenti, l. brevis, l. buchneri, l. cellobiosis, and l. salivarius subsp. salivarius. the cultural conditions under which the enzyme activity was detected suggest that the enzyme is constitutive and present in the soluble fraction in the cell. the enzyme preparations readily hydrolyzed melibiose and other oligosaccharides containing alpha(1 --> 6) linked galactose. although the cell-free extracts of l ...19734796954
studies of thiamine uptake in growing cultures and in cell fragments of lactobacillus fermenti. 19714947426
purification and properties of phosphotransacetylase from lactobacillus fermenti. 19714995446
[studies on the phosphotransacetylase of lactic acid bacteria. v. effects of some of medium constituents on the phosphotransacetylase levels of lactobacillus fermenti]. 19715105137
studies on amino acid racemases. ii. purification and properties of the glutamate racemase from lactobacillus fermenti. 19695363991
studies on amino acid racemases. i. partial purification and properties of the alanine racemase from lactobacillus fermenti. 19695367453
studies on the group f antigen of lactobacilli: isolation of a teichoic acid-lipid complex from lactobacillus fermenti nctc 6991. 19705487616
phosphotransacetylase of lactobacillus fermenti, properties of the enzyme and determination of coenzyme a. 19705499638
isolation and properties of mutants of lactobacillus fermenti resistant to amprolium. 19705513334
[studies on the phosphotransacetylase of lactic acid bacteria. ii. enzymatic preparation of acetyl-coenzyme a and propionyl-coenzyme a using phosphotransacetylase from lactobacillus fermenti]. 19705530456
cell wall and peptidoglycan from lactobacillus fermenti.cell walls from lactobacillus fermenti were prepared by differential centrifugation of disrupted cells, with and without trypsin treatment. approximately 16% of the dry weight of walls was found in a crude trichloroacetic acid extract of the walls; half of this amount remained upon further purification. the purufied extract lacked alanine, but contained substantial amounts of glucosamine. the walls constituted 23 to 33% of the dry weight of the cell. the chemical composition of the various types ...19715547995
growth promoting effect of oxythiamine on lactobacillus fermenti. 19675601992
structure of the cell wall of lactobacilli. role of muramic acid phosphate in lactobacillus fermenti.1. the polysaccharide and mucopeptide components of the cell wall of lactobacillus fermenti, serological group f, were separated by mild conditions of acid hydrolysis; the polysaccharide was composed of glucose and galactose. 2. soluble cell-wall products were isolated from cell wall lysed by lysozyme and a streptomyces enzyme preparation. the lysozyme-dissolved fraction contained a greater proportion of mucopeptide. 3. the soluble preparations were heated in dilute acid to hydrolyse the linkage ...19685667252
lactobacilli on plants.the distribution, enumeration, and identification of lactobacilli on vegetable plants were studied in an area described geographically as being subtropical and moist. the lactobacilli were obtained, by means of quantitative enrichment procedures in rogosa's sl broth, from 35.3% of all samples incubated at 32 c, and from 15.4% of the samples incubated at 45 c. less than 10 lactobacilli/g of plant material were enumerated in 54% of all positive samples. the lactobacilli were found much less freque ...19685676407
use of the stereoisomers of isoleucine by lactobacillus fermenti.the use of four stereoisomers of isoleucine by lactobacillus fermenti strain 36 was studied in detail. all four isoleucine isomers were used for growth in the presence of vitamin b(6) compounds, but only l-isoleucine was active in the absence of these vitamins. of the vitamin b(6) compounds, pyridoxal and pyridoxamine were equally more effective than pyridoxine for the utilization of these isomers. lowering the initial ph, decreasing the amounts of leucine and valine, and adapting the organism t ...19655867648
transport of b-vitamins in microorganisms. ii. factors affecting the uptake of labelled thiamine by nonproliferating cells of lactobacillus fermenti. 19665958826
transport of b-vitamins in microorganisms. 3. chromatographic studies on the radioactivity extracted from non-proliferating cells of lactobacillus fermenti after exposure to labelled thiamine. 19665958827
identification of l-ornithine and delta-aminosuccinyl ornithine in cell wall hydrolysates of lactobacillus cellobiosus. 19676031807
isolation of an ornithine-containing cell wall precursor of lactobacillus cellobiosus. 19676035494
properties of a lactobacillus fermenti bacteriocin. 19676038900
detection and activity of lactacin b, a bacteriocin produced by lactobacillus acidophilus.a total of 52 strains of lactobacillus acidophilus were examined for production of bacteriocins. a majority (63%) demonstrated inhibitory activity against all members of a four-species grouping of lactobacillus leichmannii, lactobacillus bulgaricus, lactobacillus helveticus, and lactobacillus lactis. four l. acidophilus strains with this activity also inhibited streptococcus faecalis and lactobacillus fermentum, suggesting a second system of antagonism. under conditions eliminating the effects o ...19836410990
automated microbiological assay of thiamin in serum and red cells.to develop a sensitive, direct, automated method for the measurement of serum and red cell thiamin.19948089221
identification of lactobacilli from sourdough and description of lactobacillus pontis sp. nov.the microflora of sourdough preparations was investigated by examining the physiological characteristics, whole-cell protein patterns, and 16s rrna sequences of lactobacillus isolates. strains isolated from sourdough were placed in the species lactobacillus brevis, lactobacillus sanfrancisco, and lactobacillus reuteri. 16s rrna sequences were determined for l. brevis, lactobacillus fructivorans, lactobacillus fermentum, l. sanfrancisco, and l. reuteri, and oligonucleotide probes for fast specifi ...19948186088
coaggregation of oral lactobacilli with streptococci from the oral cavity.the ability of oral lactobacilli to coaggregate with streptococci and actinomycetes was investigated. of the 7 species of lactobacilli studied, only two were capable of coaggregation and the coaggregation was restricted to streptococci. lactobacillus salivarius strains (2/4) coaggregated with streptococcus salivarius, streptococcus gordonii, streptococcus crista and tufted streptococcus sanguis ii strains. lactobacillus fermentum (2/3) coaggregated with s. gordonii and s. sanguis. the coaggregat ...19938265207
lactobacillus cells in the rabbit digestive tract and the factors affecting their distribution.lactobacillus fermentum was present in small numbers in the caecum and colon of only one of 16 rabbits. studies aimed at elucidating the factors affecting the colonization of lactobacilli in rabbit gut were thus performed. these studies included investigation of the effect of gastric juice and bile salts on the viability of lactobacilli, assay of the survival rates of lactobacilli in the gastrointestinal tracts of the rabbits with or without ileum cannulation and measurement of the adhesive capa ...19938244905
the aggregation of human platelets by lactobacillus species.the ability to aggregate human platelets was examined for five lactobacillus rhamnosus strains and five lactobacillus paracasei subsp. paracasei strains isolated from patients with infective endocarditis (ie), 25 laboratory isolates from the same two species, and 14 strains from five other oral species, namely lactobacillus acidophilus, lactobacillus fermentum, lactobacillus oris, lactobacillus plantarum and lactobacillus salivarius. amongst the l. rhamnosus strains, platelets were aggregated by ...19938126421
the binding of pneumococcal lipoteichoic acid to human erythrocytes.previous studies have evaluated the binding characteristics of lipoteichoic acids (lta) composed of polyglycerol phosphate to mammalian cell membranes. this present study was performed to evaluate the binding characteristics of pneumococcal lta, a polyribitol phosphate containing lta, to human erythrocytes. binding was found to be specific, reversible, and dependent on temperature and time. maximum binding was achieved within 2 h at 37 degrees c. scatchard plot analysis of the binding revealed a ...19938391621
[a microflora study of the gastrointestinal tract of mink housed within and outside of the area of the chernobyl atomic electric power station].quantitative differences in the content of lactic acid bacteria isolated from the content of the stomach, small and large intestine have been established when studying microflora of the gastrointestinal tract of minks kept in the 30-kilometer zone of the chernobyl npp (experimental animals) and at the cherkassy fur farm (control animals). obligate heterofermentative species of lactic acid bacteria related to lactobacillus fermentum and l. reuteri prevailed in the stomach of experimental minks. s ...19957663745
lactobacillus fermentum endocarditis involving a native mitral valve. 19957650937
induction of hypersensitivity reactions to lactobacillus fermentum and lipoteichoic acid in rabbits. part ii.regimens of intravenous injections of saline-washed lactobacillus fermentum elicited hypersensitivity reactions in rabbits. pathological investigation revealed evidence consistent with induction of aggregate anaphylaxis, characterised by acute cor pulmonale. additional evidence of similar tissue injury was observed in livers of rabbits which had received several intravenous injections of l. fermentum. deposition of immune complexes in kidney glomeruli was demonstrated in only 1 out of 11 animals ...19817239699
d-lactic acidosis in two patients with short bowel syndrome: bacteriological analyses of the fecal flora.two cases of d-lactic acidosis associated with short bowel syndrome are described. the administration of kanamycin to the patients showed a decrease in d-lactate in blood and urine in parallel with disappearance of metabolic acidosis. bacteriological analyses of the fecal flora showed an increase in lactobacillus buchneri in the first patient and lactobacillus fermenti iva in the second; both bacteria were sensitive to kanamycin. quantification of in vitro production of d-lactate by each species ...19827128641
effect of adherent lactobacillus spp. on in vitro adherence of salmonellae to the intestinal epithelial cells of chicken.single strains of lactobacillus acidophilus and lact. fermentum, isolated from chicken intestine, were used to study in vitro interactions with salmonella enteritidis, salm. pullorum or salm. typhimurium in an ileal epithelial cell (iec) radioactive assay. exclusion, competition and displacement phenomena were investigated by respectively incubating (a) lactobacilli and iec together, prior to addition of salmonellae, (b) lactobacilli, iec and salmonellae together, and (c) salmonellae and iec, fo ...19968760330
induction of igm immunological memory to lipoteichoic acids in rabbits. part i.intravenous immunisation of rabbits with 10(9) lactobacillus fermentum cells elicited a response, specific for lipoteichoic acid (lta), detectable as igm plaque-forming cells (pfc) in the spleen by day 2 and as thiol-sensitive 19s antibodies in sera by day 3. direct pfc responses peaked at day 6, with no indirect pfc demonstrable at the time. specific igg pfc appeared after 14 days. a second intravenous injection 5 weeks later induced a 10-fold higher igm pfc response to lta which reached a maxi ...19817014476
immune responses to lipoteichoic acid: comparison of antibody responses in rabbits and mice. part ii.humoral immune responses to lipoteichoic acid (lta) as a surface antigen of lactobacillus fermentum were assessed in rabbits and mice. intravenous injection of rabbits with whole bacteria was effective, over a wide range of doses (10(4) to 10(10) cells), in eliciting antibodies to lta. in mice, significant levels of anti-lta antibodies were induced only following intraperitoneal injection of 10(8) to 10(9) l. fermentum cells. rabbit antibodies to lta were predominantly of the igm class and were ...19816894440
development of a simple passive haemagglutination-inhibition assay for listeria monocytogenes lipoteichoic acid.a simple assay for listeria lipoteichoic acid was devised. it is based on the property of this substance to attach spontaneously to erythrocytes. thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. cross-reactivity of lipoteichoic acid from lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal ...19816792287
influence of carbohydrates on the alpha-galactosidase activity of lactobacillus fermentumthe influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from lactobacillus fermentum crl 251 was studied. stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; scarce activity was detected with glucose. exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of l. fermentum growing on galactose. this effect was reverse ...19968875910
a bifunctional urease enhances survival of pathogenic yersinia enterocolitica and morganella morganii at low ph.to infect a susceptible host, the gastrointestinal pathogen yersinia enterocolitica must survive passage through the acid environment of the stomach. in this study, we showed that y. enterocolitica serotype o8 survives buffered acidic conditions as low as ph 1.5 for long periods of time provided urea is available. acid tolerance required an unusual cytoplasmically located urease that was activated 780-fold by low-ph conditions. acid tolerance of helicobacter species has also been attributed to u ...19968932305
cariogenicity of human oral lactobacilli in hamsters.of 50 strains of lactobacilli isolated from dental plaque of school children, two strains, provisionally identified as lactobacillus salivarius, and one strain, provisionally identified as lactobacillus fermentum, induced significant caries activity in conventional hamsters. sucrose was a required dietary cariogenic substrate and could not be replaced with glucose or starch. in contrast to cariogenic strains of streptococcus mutans the active lactobacilli did not form adherent sucrose-mediated p ...19806767764
effects of erythromycin-inactivating lactobacillus crop flora on blood levels of erythromycin given orally to chicks.germ-free, day-old chickens were colonized with a lactobacillus fermentum strain isolated from poultry which was able to inactivate in vitro erythromycin and other macrolide and lincosamide antibiotics. similar control chickens were colonized with a non-degrading l. fermentum strain. only in chickens colonized with the non-degrading strain were blood levels considered to be therapeutically active achieved with erythromycin in the drinking water. five groups of broiler chickens of different age o ...19846708166
interaction of streptococcus mutans glucosyltransferases with teichoic acids.the streptococcus mutans gs5 glucosyltransferase activities (both water-soluble and -insoluble glucan-synthesizing fractions) were inhibited by purified lipoteichoic acid. in vitro sucrose-dependent colonization of smooth surfaces by strain gs5 was also markedly reduced in the presence of the amphipathic molecules. the inhibition of soluble glucan synthesis by lipoteichoic acid appeared to be competitive with respect to both sucrose and primer dextran t10. these inhibitory effects were dependent ...19806452411
[the antagonistic properties of microaerophilic bacteria isolated from the human and mink digestive tracts].study of antagonistic properties of microaerophilic bacteria isolated from human and mink gastroenteric tract have helped to establish differences in species composition, quantity and level of antagonistic activity of the studied microorganisms in respect to pathogenic microflora. it is shown that lactic acid bacteria identified as lactobacillus fermentum and l. reuteri prevail among the strains isolated from the stomach and thin intestine of minks kept in the 30-km zone of chernobyl npp. specie ...19969044713
interaction of anti-kojibiose antibody with the lipoteichoic acids from streptococcus faecalis and streptococcus faecium.antisera prepared in rabbits by immunization with p-aminophenyl beta-kojibioside conjugated to bovine serum albumin (antikojibiose sera), readily agglutinated whole cells of streptococcus faecalis or streptococcus faecium, and showed specific reactions with the lipoteichoic acids (ltas) of these streptococci by passive hemagglutination, microscale enzyme-linked immunosorbent assay, and crossed immunoelectrophoresis. the interaction of the antikojibiose sera with the ltas was inhibited best by ko ...19846434429
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