| degradation of sinigrin by lactobacillus agilis strain r16. | forty-two lactobacilli were screened for their potential to degrade glucosinolate sinigrin. one of them, strain r16, demonstrated a high level of sinigrin degradation; it was identified as lactobacillus agilis. the sinigrin degrading activity of l. agilis r16 could only be demonstrated when intact cells were used. the products of sinigrin degradation are allyl-isothiocyanate (aitc) and glucose (which is further fermented to dl-lactic acid), suggesting that myrosinase activity is involved. the ac ... | 1995 | 7577359 |
| lactobacillus agilis is an important component of the pigeon crop flora. | aims: to examine the presence of lactic acid bacteria (lab: enterococci, streptococci and lactobacilli) in the pigeon crop. methods and results: the crops of 10 pigeons were sampled and inoculated on agar plates for isolation of streptococci, enterococci and lactobacilli. the isolates were identified using tdna-pcr. lactobacillus agilis, a species described in 1981 from municipal sewage, was the dominant component in eight of these pigeon crop sacs. a lactobacillus species related to l. fermentu ... | 2001 | 11556914 |
| impact of two probiotic lactobacillus strains feeding on fecal lactobacilli and weight gains in chicken. | two probiotic strains, lactobacillus agilis jcm 1048 and l. salivarius subsp. salicinius jcm 1230 isolated from chicken intestine, exhibited probiotic characteristics that can be applied for chicken production. after 7 days of probiotic feeding (fd7), the count of intestinal lactobacilli in the probiotic group (group p, n=10) was significantly (p<0.05) higher than that in the control group (group c, n=9). after 40 days of probiotic feeding (fd40), the lactobacilli and enterococci counts were sta ... | 2003 | 12682864 |
| effects of two probiotic lactobacillus strains on jejunal and cecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16s rrna genes. | we examined the effects of probiotic lactobacillus strains of lactobacillus agilis jcm 1048 and lactobacillus salivarius subsp. salicinius jcm 1230 on jejunal and cecal microbiota of broiler chicken under heat stress condition using terminal restriction fragment length polymorphism (t-rflp) analysis. the jejunal bacterial community was limited to a few bacterial groups, mostly lactobacillus spp. a relatively abundant and higher prevalence of lactobacillus spp. were observed in the jejunal and ce ... | 2004 | 15611608 |
| influence of intensive and extensive breeding on lactic acid bacteria isolated from gallus gallus domesticus ceca. | in the present study, lactic acid bacteria (lab) from the cecum of chickens bred either under intensive (commercial broilers) or extensive (free-range) conditions were isolated, identified and some of their probiotic characteristics determined. the lab identified by 16s-23s rrna pcr-ardra were mainly of lactobacillus species and to a lesser extent of enterococcus spp. for all animals. free-range chickens showed a higher presence of lactobacillus acidophilus while lactobacillus reuteri and lactob ... | 2007 | 17113730 |
| yeasts and lactic acid bacteria microbiota from masau (ziziphus mauritiana) fruits and their fermented fruit pulp in zimbabwe. | masau are zimbabwean wild fruits, which are usually eaten raw and/ or processed into products such as porridge, traditional cakes, mahewu and jam. yeasts, yeast-like fungi, and lactic acid bacteria present on the unripe, ripe and dried fruits, and in the fermented masau fruits collected from muzarabani district in zimbabwe were isolated and identified using physiological and molecular methods. the predominant species were identified as saccharomyces cerevisiae, issatchenkia orientalis, pichia fa ... | 2007 | 17904237 |
| in vitro fermentation of broiler cecal content: the role of lactobacilli and ph value on the composition of microbiota and end products fermentation. | to assess the probiotic effects of lactobacillus agilis jcm 1048 and l. salivarius ssp. salicinius jcm 1230 and the ph on the cecal microflora of chicken and metabolic end products. | 2009 | 19725887 |
| selected microbial groups and short-chain fatty acids profile in a simulated chicken cecum supplemented with two strains of lactobacillus. | among the bacterial fermentation end products in the chicken cecum, butyrate is of particular importance because of its nutritional properties for the epithelial cell and pathogen inhibitory effects in the gut. an in vitro experiment, operated with batch bioreactor, was conducted to quantify butyric-producing bacteria in a simulated broiler cecum supplemented with lactobacillus salivarius ssp. salicinius jcm 1230 and lactobacillus agilis jcm 1048 during 24 h of incubation. selected bacterial spe ... | 2010 | 20181862 |
| a small variation in diet influences the lactobacillus strain composition in the crop of broiler chickens. | feed composition has the potential to influence the activities of bacteria that colonize the digestive tract of broiler chickens with important consequences for animal health, well being, and food safety. in this study, the gut microbiota of two groups of broiler chickens raised in immediate vicinity but fed either a standard corn/soybean meal ration (corn-soy, cs) or a ration high in wheat middlings (high wheat, hw) was characterized. the findings revealed that this small variation in feed comp ... | 2010 | 20554146 |
| lactobacillus strain ecology and persistence within broiler chickens fed different diets: identification of persistent strains. | lactobacilli are autochthonous residents in the chicken gastrointestinal tract, where they may potentially be used as probiotics, competitive exclusion agents, or delivery vehicles. the aim of this study was to use an in vivo model to investigate the effect of diet and competing lactic acid bacteria on the colonization of inoculated lactobacillus strains, with the goal of identifying strains which can consistently colonize or persist for an extended period of several weeks. chicken-derived lacto ... | 2010 | 20693442 |
| application of the biorefinery concept to produce l-lactic acid from the soybean vinasse at laboratory and pilot scale. | lactic acid is a product that finds several applications in food, cosmetic, pharmaceutical and chemical industries. the main objective of this work was the development of a bioprocess to produce l(+)-lactic acid using soybean vinasse as substrate. among ten strains, lactobacillus agilis lpb 56 was selected for fermentation, due to its ability to metabolize the complex oligosaccharides. fermentation was conducted without need for supplementary inorganic nitrogen sources or yeast extract. kinetic ... | 2010 | 20933391 |
| transformation of, and heterologous protein expression in, lactobacillus agilis and lactobacillus vaginalis isolates from the chicken gastrointestinal tract. | lactobacilli are naturally found in the gastrointestinal tract of chickens, and there is interest in utilizing autochthonous strains for the delivery of therapeutic proteins. previously we identified three chicken-derived lactobacillus strains, lactobacillus agilis la3, lactobacillus vaginalis lv5, and lactobacillus crispatus lc9, which persist in the gastrointestinal tract of chickens fed either a commercial or high-protein diet. in the current study, we investigated the ability to electrotrans ... | 2010 | 21075881 |
| screening for lactic acid bacteria capable of inhibiting campylobacter jejuni in in vitro simulations of the broiler chicken caecal environment. | thermotolerant campylobacter spp., specifically campylobacter jejuni and campylobacter coli, are the most common bacterial causes of human gastroenteritis in developed countries. consumption of improperly prepared poultry products and cross contamination are among the main causes of human campylobacteriosis. the aim of this study was to identify lactic acid bacterial (lab) strains capable of inhibiting c. jejuni growth in initial in vitro trials ('spot-on-lawn' method), as well as in batch ferme ... | 2012 | 23234730 |
| glucosinolate and desulfo-glucosinolate metabolism by a selection of human gut bacteria. | glucosinolate (gsl) hydrolysis is mediated by the enzyme myrosinase which together with specifier proteins can give rise to isothiocyanates (itcs), thiocyanates, and nitriles (nits) in cruciferous plants. however, little is known about the metabolism of gsls by the human gut flora. the aim of the work was to investigate the metabolic fates of sinigrin (sng), glucotropaeolin (gtp), gluconasturtiin (gnt), and their corresponding desulfo-gsls (ds-gsls). three human gut bacterial strains, enterococc ... | 2016 | 27301252 |
| the metabolism of methylsulfinylalkyl- and methylthioalkyl-glucosinolates by a selection of human gut bacteria. | certain myrosinase-positive human gut bacteria can metabolize glucosinolates (gsls) to produce isothiocyanates (itc) as chemopreventive agents. we investigated glucoerucin, glucoiberin, and glucoraphanin (present in broccoli) metabolism by human gut strains. | 2014 | 24170324 |
| identification of proteins possibly involved in glucosinolate metabolism in l. agilis r16 and e. coli vl8. | this study was aimed to identify sinigrin-induced bacterial proteins potentially involved in the metabolism of glucosinolate in two glucosinolate-metabolising bacteria lactobacillus agilis r16 and escherichia coli vl8. sinigrin (2 mm) was used to induce the proteins in both bacteria under anaerobic incubation for 8 h at 30 °c for l. agilis r16 and 37 °c for e. coli vl8 and the controls without sinigrin were performed. allyl isothiocyanate and allyl nitrile as two degradation products of sinigrin ... | 2015 | 25805049 |
| in vitro evaluation of the safety and probiotic properties of lactobacilli isolated from chicken and calves. | a total of 73 chicken and calves isolates were diagnosed using matrix-assisted laser desorption ionization-time-of flight mass spectrometry (maldi-tof ms). after a preliminary subtractive screening based on the high acid tolerance at ph 2.5 and bile resistance at 0.3% oxgall, twenty isolates belonging to the species lactobacillus salivarius, lactobacillus agilis, lactobacillus reuteri, lactobacillus murinus and lactobacillus amylovorus were in vitro screened for the safety assessment and probiot ... | 2014 | 24291759 |
| effect of supplementation of yeast with bacteriocin and lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens. | the aim of the present study was to investigate the effect of yeast with bacteriocin and lactobacillus cultures (mixture of lactobacillus agilis bcrc 10436 and lactobacillus reuteri bcrc 17476) supplements, alone or in combination, on broiler chicken performance. | 2017 | 27383813 |
| characterization of flagellins isolated from a highly motile strain of lactobacillus agilis. | most lactic acid bacteria are non-motile but some of them are flagellated and exhibit motility. so far, motile lactobacilli have rarely been studied, and characteristics of their flagellins are poorly understood. in this study, a highly motile strain of lactobacillus agilis was recruited for transcriptional analysis and characterization of its flagellins. | 2016 | 27001290 |
| draft genome sequence of the lactobacillus agilis strain marseille. | we report the draft genome sequence of lactobacillus agilis strain marseille, isolated from stool samples of a child suffering from kwashiorkor. this strain can use two metabolic pathways allowing the assimilation of glucose and xylose. here, we present the first draft genome of the lactobacillus agilis species. | 2015 | 26227602 |
| identification of lactobacillus strains of goose origin using maldi-tof mass spectrometry and 16s-23s rdna intergenic spacer pcr analysis. | the objective of our study was to identify lactobacillus sp. strains of goose origin using maldi-tof mass spectrometry, its-pcr and its-pcr/rflp. all three techniques proved to be valuable tools for identification of avian lactobacilli and produced comparable classification results. lactobacillus strains were isolated from 100% of geese aged 3 weeks to 4 years, but from only 25% of chicks aged 1-10 days. among the 104 strains isolated, we distinguished 14 lactobacillus species. the dominant spec ... | 2014 | 24607713 |
| the effects of liquid versus spray-dried laminaria digitata extract on selected bacterial groups in the piglet gastrointestinal tract (git) microbiota. | in this study, the effects of supplementing animal feed with a liquid and spray-dried fucoidan and laminarin extract, derived from the seaweed laminaria digitata on the porcine gastrointestinal microbiota, specifically the communities of lactobacillus, bifidobacterium and enterobacteria were evaluated. twenty four piglets were fed one of three diets over a 21-day period to determine the effect that each had on the bacterial communities. the dietary treatments were as follows; (1) control diet, ( ... | 2013 | 23542115 |
| supplemental thymol and carvacrol increases ileum lactobacillus population and reduces effect of necrotic enteritis caused by clostridium perfringes in chickens. | necrotic enteritis (ne) caused by clostridium perfringens is one of the most detrimental infectious diseases in poultry. this study examined the effect of blends of essential oils (beos) (25% thymol and 25% carvacrol) on ne and bacterial dynamics and functions in chicks challenged with c. perfringens. chicks were assigned to a control diet and beos diet (control diet + 120 mg/kg beos), were challenged with c. perfringens from days 14 to 20 and were killed on day 21 for assessment. supplementatio ... | 2017 | 28779076 |