| binding of lactobacillus reuteri to fibronectin immobilized on glass beads. | human fibronectin was immobilized on glass beads. the beads were used to evaluate binding of lactobacillus reuteri to fibronectin. organisms bound to the glass beads were detected using fluorescence microscopy after treatment with acridine orange. this binding was confirmed and quantified with the use of [3h]-labelled organisms. three strains of lactobacillus reuteri, three strains of lactobacillus acidophilus and one strain of lactobacillus fermentum were tested for binding capacity. l. reuteri ... | 1992 | 1303695 |
| systematics of the lactobacillus population on rat intestinal mucosa with special reference to lactobacillus reuteri. | the systematics of the lactobacillus population of the intestines of 88 different rats was studied; 80 rats had been fed on fermented oat-meal soup (molin et al. 1992). one-hundred-twenty-two lactobacillus strains from the intestinal mucosa were phenotypically classified together with twenty-eight reference strains of lactobacillus and leuconostoc, using 49 unit characters. data were examined using jaccard coefficient, and unweighted pair group algorithm with arithmetic averages. two major and e ... | 1992 | 1325752 |
| effect of fermented oatmeal soup on the cholesterol level and the lactobacillus colonization of rat intestinal mucosa. | rats were fed with freeze-dried oatmeal soup fermented by six different lactobacillus strains from rat and man; the formula is intended for enteral feeding. the serum cholesterol levels after 10 d were lower for rats eating oatmeal as compared to a commercial product, biosorb sond. colonizing ability of the administered strains were evaluated in vivo. only lactobacillus reuteri r21c were able to, effectively, colonizing the mucosa; it represented about 30% of the lactobacillus population 24 d af ... | 1992 | 1519914 |
| characterization of gram-positive broad host-range plasmids carrying a thermophilic replicon. | the cryptic plasmid pbc1 (1.6 kb) isolated from bacillus coagulans zu1961 was genetically marked with the genes for chloramphenicol and ampicillin resistance (cmr and apr) from the escherichia coli plasmid pjh101. the recombinant vector obtained (pcp49, 7.0 kb) replicated and expressed cmr in b. subtilis and cmr and apr in e. coli. different shuttle vectors for gram+ bacteria were also constructed by inserting pbc1 into the staphylococcus aureus plasmid pc194. the smallest of these, plm6 (2.8 kb ... | 1991 | 1908113 |
| single-stranded dna plasmid, vector construction and cloning of bacillus stearothermophilus alpha-amylase in lactobacillus. | vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to taqi-digested dna of a cryptic plasmid from lactobacillus plantarum. the minimal region of lactobacillus plasmid dna that was required for dna replication was defined and a single-stranded dna intermediate replication system was observed. homologies with other origins of replication of plasmids from gram-positive bacteria, replicating via rolling circle mechanism, were found. it was shown that the c ... | 1991 | 1961976 |
| antagonistic activities of lactic acid bacteria in food and feed fermentations. | many factors contribute to a successful natural fermentation of carbohydrate-rich food and feed products. metabolic activities of lactic acid bacteria (lab) play a leading role. their ability to rapidly produce copious amounts of acidic end products with a concomitant ph reduction is the major factor in these fermentations. although their specific effects are difficult to quantitate, other lab metabolic products such as hydrogen peroxide and diacetyl can also contribute to the overall antibiosis ... | 1990 | 2125429 |
| [effects of heat treated cells of intestinal lactic acid bacteria in rats fed a deoxycholic acid diet]. | effects of the administration of heat treated cells of intestinal lactic acid bacteria were examined in rats fed a deoxycholic acid (dca) diet. male wistar rats were given a 0.25% dca diet with or without the heat treated cells of enterococcus faecalis ad 1001 (efh-1) or lactobacillus reuteri ad 0002 (lrh-2) for 4 weeks. abnormal increases in serum got, gpt, un and lipoproteins were observed in the rats fed the dca diet. furthermore, severe lesions in the kidney as well as in the liver were foun ... | 1990 | 2401316 |
| antagonism and adhesion among isogenic strains of lactobacillus reuteri in the caecum of gnotobiotic mice. | interactions between a wild adhesive lactobacillus reuteri strain isolated from chicken crop and its isogenic plasmid-free derivative were studied. antagonism between these strains was tested in vitro or associated with gnotobiotic mice. the cured strains repressed the establishment of the wild, plasmid bearing strain in the gastrointestinal tract of gnotobiotic mice. interactions between isogenic strains were independent of the amount of cells administered to mice. the cured derivative strain m ... | 1989 | 2716537 |
| biotin-labeled plasmid dna probes for detection of epithelium-associated strains of lactobacilli. | biotin-labeled dna probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (lactobacillus delbrueckii 21 and lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. the forestomach sections were incubated on nylon filter membranes placed on agar plates and, after lysis of the lactobacillus cells and denaturation of their dna, were used in hybridization experiments with the strain-spe ... | 1989 | 2719480 |
| chemical characterization of an antimicrobial substance produced by lactobacillus reuteri. | lactobacillus reuteri converts glycerol into a potent cell growth inhibitor. this substance, termed reuterin, inhibits the growth of gram-positive and gram-negative bacteria as well as yeasts, fungi, and protozoa. semipreparative chromatography was used to purify reuterin, and fourier transform infrared spectroscopy and liquid chromatography-mass spectrometry were used to establish the molecular weight as well as the molecular functionality of the reuterin molecule. nuclear magnetic resonance st ... | 1989 | 2751282 |
| in vivo transfer of pam beta 1 from lactobacillus reuteri to enterococcus faecalis. | trials were conducted to determine the in vivo transferability of plasmid-mediated antibiotic resistance between two strains of enteric gram-positive bacteria. germ-free mice were associated with the donor lactobacillus reuteri dsm 20016 strain, carrying the broad host range pam beta 1 plasmid, and with the enterococcus faecalis jh2ss recipient strain. analysis of faecal content of associated mice demonstrated that the in vivo transfer of this plasmid did occur and that frequencies of conjugatio ... | 1988 | 2976754 |
| chitosan-colloidal gold complexes as polycationic probes for the detection of anionic sites by transmission and scanning electron microscopy. | a new cationic colloidal gold complex has been developed for ultrastructural localization of cell surface anionic sites by transmission and scanning electron microscopy. the marker is prepared by labelling gold particles of suitable sizes (6 to 70 nm in diameter) with chitosan, a polymer of beta (1----4)-linked d-glucosamine. using human red blood cells as a model, chitosan-gold complexes were shown to be specific for anionic sites and at ph 2 for sialic acid residues. the binding capacity of co ... | 1988 | 3063695 |
| conjugal transfer of plasmid pam beta 1 in lactobacillus reuteri and between lactobacilli and enterococcus faecalis. | the broad-host-range plasmid pam beta 1 (erythromycin resistance) was transferred conjugally from streptococcus lactis to lactobacillus reuteri, l. murinus, and l. fermentum. transfer of pam beta 1 between two l. reuteri strains occurred, and lactobacillus transconjugants could act as donors of pam beta 1 in crosses with enterococcus faecalis jh2-2. | 1987 | 3122658 |
| identification and cloning of a plasmid-encoded erythromycin resistance determinant from lactobacillus reuteri. | plasmid analysis, plasmid curing, cloning, and hybridization experiments were used to study four lactobacillus reuteri strains showing high resistance to erythromycin. plasmid curing with acriflavine resulted in a loss of erythromycin resistance in a frequency of 1-10%. for three of the strains this was accompanied by a loss of a 6.9-mda plasmid, which was shown to be identical for the different strains and designated plul631. the erythromycin (erm) gene was located on a 5.5-mda plasmid in the f ... | 1988 | 3237864 |
| production and isolation of reuterin, a growth inhibitor produced by lactobacillus reuteri. | lactobacillus reuteri is a prominent member of the lactobacillus population in the gastrointestinal ecosystem of humans, poultry, swine, and other animals. reuterin is a newly discovered, broad-spectrum antimicrobial substance produced by this species during fermentation of glycerol. in this report, we describe procedures for (i) producing reuterin in sufficient amounts to isolate from a fermentation mixture and (ii) isolating this substance by high-performance liquid chromatography. by using un ... | 1988 | 3245697 |
| formation and regeneration of protoplasts and spheroplasts of gastrointestinal strains of lactobacilli. | methods were developed for the formation of protoplasts and spheroplasts of gastrointestinal strains of lactobacillus reuteri, lactobacillus gasseri, and lactobacillus salivarius. attempts to regenerate vegetative cells from protoplasts were not successful, but spheroplasts could be regenerated consistently for five of six strains. | 1988 | 3415227 |
| study on crop adhesion genetic determinant in lactobacillus reuteri. | the genetic determinant responsible for adherence in one strain of lactobacillus reuteri isolated from chicken crop has been investigated. after curing experiments carried out on this strain, a plasmid-free derivative was obtained, resulting unchanged as regards adhesion ability and carbohydrate fermentation pattern, but showing a loss of antibiotic resistances. it is suggested a possible localization on chromosome of adhesion determinant. | 1986 | 3747852 |
| drug resistance plasmids in lactobacillus acidophilus and lactobacillus reuteri. | sixteen strains of lactobacillus reuteri and 20 strains of lactobacillus acidophilus were tested for resistance to 22 antibiotics by using commercially available sensitivity disks. evidence suggesting linkage of these resistances to plasmids was obtained by "curing" experiments with acridine dyes and high growth temperatures. examination of plasmid patterns of agarose gel electrophoresis provided further evidence of loss in plasmid dna under curing conditions in some of the strains examined. | 1982 | 6798933 |
| development of intestinal lactobacilli in normal piglets. | the development of intestinal lactobacillus spp. in 18 normal piglets from six litters was investigated from 1 to 45 d after birth. faecal lactobacillus strains isolated from piglets and their dams were identified at species and biovar levels on the basis of carbohydrate fermentation. a definite pattern was observed in the changes of four species and 20 biovars of lactobacilli appearing in faeces, with growth of the piglets. lactobacillus reuteri colonized the animals on the first day of birth. ... | 1995 | 7592119 |
| cloning of promoter-like sequences from lactobacillus paracasei subsp. paracasei cg11 and their expression in escherichia coli, lactococcus lactis, and lactobacillus reuteri. | fragments of chromosomal dna from lactobacillus paracasei subsp. paracasei cg11 (formerly lactobacillus casei cg11) capable of functioning as promoters were isolated using the broad host range, promoter-probe vector pgkv210. five such fragments designated p61, p79, p80, p116, and p144 were completely sequenced and analyzed. fragment p61 had the highest transcriptional efficiency in escherichia coli and lactobacillus reuteri whereas p80 was the most active in lactococcus lactis. in general, the o ... | 1994 | 7704831 |
| the role of oral administration of oatmeal fermented by lactobacillus reuteri r2lc on bacterial translocation after acute liver failure induced by subtotal liver resection in the rat. | previous experimental studies showed that a disturbed ecology of the enteric bacterial population might contribute to the occurrence of bacterial translocation from the gut in acute liver failure (alf). | 1995 | 7732342 |
| cloning of the gene for cholesterol oxidase in bacillus spp., lactobacillus reuteri and its expression in escherichia coli. | the cloning of the cholesterol oxidase gene in several gram-positive bacteria, including lactobacillus reuteri of intestinal origin, was obtained. only the transformants of escherichia coli harbouring the recombinant plasmid pchoa showed a good intracellular enzyme activity. the heterologous gene was stably maintained in gram-positive transformants but no enzyme activity was detected. | 1993 | 7763933 |
| purification of collagen-binding proteins of lactobacillus reuteri ncib 11951. | collagen type-i-binding proteins of lactobacillus reuteri ncib 11951 were purified. the cell surface proteins were affinity purified on collagen sepharose and eluted with an nacl gradient. two protein bands were eluted from the column (29 kda and 31 kda), and both bound radio-labeled collagen type i. rabbit antisera raised against the 29 kda and 31 kda protein reacted with the affinity-purified proteins in a western blot with whole-cell extract used as antigen. the n-terminal sequence of the 29- ... | 1994 | 7764651 |
| molecular characterization of a plasmid-borne (pgt633) erythromycin resistance determinant (ermgt) from lactobacillus reuteri 100-63. | lactobacillus reuteri 100-63 contains a 9.8 kb plasmid (pgt633) encoding resistance to the macrolide-lincosamide-streptogramin (mls) type b antibiotics. the restriction endonucleases acci, bamhi, bcli, ecori, ecorv, hhai, hindii, hpai, kpni, sali, and sspi were employed to establish a physical map of pgt633. next, genetic transfer experiments were conducted to establish the host range of pgt633. the results revealed that pgt633 replicated autonomously and encoded constitutive resistance to eryth ... | 1994 | 8171126 |
| identification of lactobacilli from sourdough and description of lactobacillus pontis sp. nov. | the microflora of sourdough preparations was investigated by examining the physiological characteristics, whole-cell protein patterns, and 16s rrna sequences of lactobacillus isolates. strains isolated from sourdough were placed in the species lactobacillus brevis, lactobacillus sanfrancisco, and lactobacillus reuteri. 16s rrna sequences were determined for l. brevis, lactobacillus fructivorans, lactobacillus fermentum, l. sanfrancisco, and l. reuteri, and oligonucleotide probes for fast specifi ... | 1994 | 8186088 |
| the effect of exogenous administration of lactobacillus reuteri r2lc and oat fiber on acetic acid-induced colitis in the rat. | the potential beneficial effect of exogenous administration of lactobacillus on acetic acid-induced colitis was evaluated in the rat. colitis was induced by instillation of 4% acetic acid for 15 sec in an exteriorized colonic segment. this produced uniform colitis with a threefold increase in myeloperoxidase (mpo) activity of the colonic tissue (an index of neutrophil infiltration) and a sixfold increase in plasma exudation into the lumen of the colon (mucosal permeability) as evaluated 4 days a ... | 1993 | 8382837 |
| aggregation-promoting factor in pig intestinal lactobacillus strains. | autoaggregation was frequently encountered among intestinal lactobacilli isolated from weaned pigs. the aggregation mechanism was shown to be mediated by the production of a proteinaceous aggregation-promoting factor in two strains of lactobacillus reuteri. a 32 kda aggregation-promoting protein was detected in these strains by cross-reaction with rabbit polyclonal antibodies for aggregation-promoting factor produced by the human isolate lact. plantarum 4b2. coaggregation reactions of lact. reut ... | 1995 | 8554760 |
| the effects of lactobacillus strains and oat fiber on methotrexate-induced enterocolitis in rats. | administration of methotrexate to rats on an elemental diet results in severe enterocolitis and death. lactobacilli, an integral part of the healthy gastrointestinal microecology, may provide therapeutic benefits to help the recovery from enterocolitis. the purpose of this study was to evaluate the effects of lactobacilli and oatbase on methotrexate-induced enterocolitis in rats. | 1996 | 8690198 |
| a collagen binding protein from lactobacillus reuteri is part of an abc transporter system? | the gene coding for a collagen binding protein from lactobacillus reuteri ncib 11951 was cloned and sequenced. a genomic lambda library was constructed and recombinant plaques were screened using antisera raised against purified collagen binding proteins from the same l. reuteri strain. the positive plaques were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis, which revealed the expression of a 29 kda protein, which reacted with the antisera and b ... | 1996 | 8870249 |
| the effect of pretreatment with endotoxin and lactobacillus on bacterial translocation in acute liver injury. | to evaluate the effect of pretreatment with endotoxin or lactobacillus reuteri pretreatment on bacterial translocation after acute liver injury. | 1996 | 8874160 |
| molecular characterization of a plasmid-borne (ptc82) chloramphenicol resistance determinant (cat-tc) from lactobacillus reuteri g4. | lactobacillus reuteri g4 contains a 7.0-kb plasmid (ptc82) encoding resistance to chloramphenicol (cm). determination of the nucleotide sequence of the genetic determinant (cat-tc) encoding resistance to cm on ptc82 revealed an open reading frame for a 238-amino-acid cm acetyltransferase (cat) monomer. this structural cat gene, 714 bp in length, was highly related (ca. 95% nucleotide and ca. 81% amino acid identity) to the 648-bp cat gene from staphylococcus aureus plasmid pc194. a total of 6 bp ... | 1996 | 8954883 |
| effect of lactobacillus reuteri on intestinal resistance to cryptosporidium parvum infection in a murine model of acquired immunodeficiency syndrome. | efficacy of lactobacillus reuteri as a probiotic for the control of cryptosporidium parvum infection was evaluated in c57bl/6 female mice that were immunosuppressed by intraperitoneal inoculation with the lp-bm5 leukemia virus. four months after inoculation, mice developed lymphadenopathy, splenomegaly, and susceptibility to c. parvum infection. after daily prefeeding with l. reuteri (10(8) cfu/day) for 10 days, mice were challenged with 6.5 x 10(6) c. parvum oocysts and fed l. reuteri during th ... | 1997 | 8985225 |
| principles of ex ovo competitive exclusion and in ovo administration of lactobacillus reuteri. | the data that have been presented indicate that the in ovo use of competitive exclusion (ce) agents is feasible for both chickens and turkeys. however, there are many pitfalls that await the use of in ovo application of ce agents, including the use of nonspecies-specific intestinal microbes and the use of harmful proteolytic, gas-producing and toxin-producing intestinal microbes. of the potential ce agents that have posthatch application, only lactobacillus reuteri has been shown to be safe and ... | 1997 | 9037704 |
| production, purification and characterization of reutericin 6, a bacteriocin with lytic activity produced by lactobacillus reuteri la6. | a bacteriocin (reutericin 6) produced by lactobacillus reuteri la6, was purified by hydrophobic chromatography from the modified mrs broth (d'-mrs) with 6180-fold increase in specific activity with 14% recovery. the molecular weight of reutericin 6 was determined to be 2.7 kda by sds-page and esi-ms. by amino acid analysis, reutericin 6 comprised of 67% hydrophobic and polar neutral amino acids. lanthionine was not detected. the lytic activity against lactobacillus delbrueckii subsp. bulgaricus ... | 1997 | 9039561 |
| cloning and molecular analysis of promoter-like sequences isolated from the chromosomal dna of lactobacillus acidophilus atcc 4356. | promoter-like sequences from the chromosomal dna of thermophilic strain lactobacillus acidophilus atcc 4356 were cloned. analysis of the three dna fragments showing promoter activity, designated p3, p6, and p15, were performed in lactobacillus reuteri, lactococcus lactis, and e. coli. the reporter cat-86 gene was expressed in all three bacterial species under control of the fragments p3 and p6. fragment p15 showed promoter activity only in lactobacillus reuteri and e. coli but not in lactococcus ... | 1997 | 9057296 |
| effect of oral supplementation of lactobacilli on bacterial translocation in acute liver injury induced by d-galactosamine. | bacterial infections and sepsis are frequent complications of acute liver injury, with a high share in the mortality and morbidity of this condition. bacterial translocation from the gut may play an important role in the high rate of infections observed. in this experiment the effect of different oral supplementation on bacterial translocation was evaluated in acute liver injury induced by d-galactosamine in the rat. | 1997 | 9059965 |
| quantitation by enzyme immunoassay of spirosin from lactobacillus reuteri and escherichia coli. | three eia methods (direct, indirect and sandwich eia) were studied to quantify spirosin in lactobacillus reuteri and escherichia coli cultured under various conditions in an attempt to get some insight into the function of spirosome. both direct and indirect eia were suited well for the quantitation of l. reuteri spirosin while direct eia was appropriate for spirosin of e. coli. sandwich eia could not be applied successfully in either case. by use of these methods, the amounts of spirosin produc ... | 1997 | 9090155 |
| lactobacillus reuteri as a therapeutic agent in acute diarrhea in young children. | certain strains of lactobacilli may promote recovery from acute diarrhea. lactobacillus reuteri is of human origin and is a natural colonizer of gastrointestinal tract. in this trial, exogenously administered l. reuteri was studied as a therapeutic agent in acute diarrhea. | 1997 | 9144122 |
| colonization of congenitally immunodeficient mice with probiotic bacteria. | we assessed the capacity of four probiotic bacteria (lactobacillus acidophilus, lactobacillus reuteri, lactobacillus casei gg, and bifidobacterium animalis) to colonize, infect, stimulate immune responses in, and affect the growth and survival of congenitally immunodeficient gnotobiotic beige-athymic (bg/bg-nu/nu) and beige-euthymic (bg/bg-nu/+) mice. the bacteria colonized and persisted, in pure culture, in the alimentary tracts of both mouse strains for the entire study period (12 weeks). alth ... | 1997 | 9234796 |
| cloning and expression of an endo-1,3-1,4-beta-glucanase gene from bacillus macerans in lactobacillus reuteri. | strains of the gastrointestinal species lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglm) of bacillus macerans. the enzyme was expressed and secreted by the lactobacilli. a plasmid construct containing the bglm gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector. | 1997 | 9251227 |
| influence of bile on beta-galactosidase activity and cell viability of lactobacillus reuteri when subjected to freeze-drying. | the effect of bile on beta-galactosidase activity and cell viability was investigated using two strains of lactobacillus reuteri that were subjected to freeze-drying. in the presence of 0.15% oxgall, beta-galactosidase activity of the whole cells was significantly increased. after lyophilization, the cultures that had been treated with oxgall showed a low survival rate without changes in beta-galactosidase activity. the poor resistance of the cells to damage from freeze-drying might be related t ... | 1997 | 9313134 |
| biotherapeutic effects of probiotic bacteria on candidiasis in immunodeficient mice. | four species of probiotic bacteria were assessed for their capacities to protect athymic bg/bg-nu/nu and euthymic bg/bg-nu/+ mice from mucosal and systemic candidiasis. each bacterial species and candida albicans colonized the gastrointestinal tracts of both strains of mice. the presence of probiotic bacteria (lactobacillus acidophilus, lactobacillus reuteri, lactobacillus casei gg, or bifidobacterium animalis) in the gastrointestinal tracts prolonged the survival of adult and neonatal bg/bg-nu/ ... | 1997 | 9317023 |
| bacteriotherapy with lactobacillus reuteri in rotavirus gastroenteritis. | certain lactic acid bacteria may accelerate recovery from acute diarrhea. lactobacillus reuteri is a commonly occurring lactobacillus species with therapeutic potential in diarrhea. | 1997 | 9427453 |
| in vitro studies of porcine lactobacilli for possible probiotic use. | fourteen strains of lactobacilli isolated from the gut of suckling pigs were identified and studied to determine their susceptibility to antimicrobial feed additives, acid tolerance, adherence to epithelial cells from the porcine intestine and antimicrobial activity. four strains were identified as lactobacillus casei subsp. casei, two strains as lactobacillus rhamnosus as well as lactobacillus reuteri and three strains as lactobacillus salivarius. the remaining strains could not be identified. ... | 1997 | 9451838 |
| cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity. | the effect of feeding live lactobacillus reuteri cells containing active bile salt hydrolase (bsh) on plasma cholesterol levels was studied in pigs. during an experiment lasting 13 weeks, twenty pigs were fed on a high-fat, high-cholesterol, low-fibre for the first 10 weeks, and a regular pig diet for the last 3 weeks. one group of animals received, twice daily, 11.25 (sd 0.16) log10 colony forming units of the potential probiotic bacteria for 4 weeks (from week 3 until week 7). from week 8 onwa ... | 1998 | 9536863 |
| characterisation and selection of probiotic lactobacilli for a preliminary minipig feeding trial and their effect on serum cholesterol levels, faeces ph and faeces moisture content. | three out of 297 lactobacillus strains isolated from pig faeces were selected for a feeding trial on account of their high bile-salt hydrolase (bsh) activity, bile-salt resistance, low ph tolerance and the production of antimicrobial substances. two strains were identified as lactobacillus johnsonii and one as lactobacillus reuteri by dna-dna hybridisation. l. johnsoniii bfe 1061 produced a bacteriocin active against a range of lactic acid bacteria (lab) and nonrelated bacteria including clostri ... | 1998 | 9600615 |
| release of spirosin associated with potassium phosphate-induced autolysis in lactobacillus reuteri dsm 20016. | we have studied the effect of medium components on spirosin production in lactobacillus reuteri dsm 20016 to get some insight into the function of spirosome in lactobacillus. among those investigated, only potassium phosphate was found to influence the production of spirosin. though it was not effective at concentrations lower than 0.6%, it promoted the spirosin production and its accumulation into culture medium in particular, at higher concentrations. the accumulation has been demonstrated to ... | 1998 | 9602533 |
| haemagglutination and glycolipid-binding activities of lactobacillus reuteri. | the carbohydrate-binding activity of lactobacillus reuteri was studied by haemagglutination (ha), ha inhibition and thin layer chromatography (tlc) overlay assays. three of the six lact. reuteri strains examined showed ha activity. two strains (jcm1081 and jcm1112t) agglutinated neuraminidase-treated, but not untreated, erythrocytes. strain jcm2762 agglutinated both treated and untreated erythrocytes. the ha activity of jcm 1081 was inhibited by galactose, lactose, methyl beta-galactoside and as ... | 1998 | 9750315 |
| evidence for hypocholesterolemic effect of lactobacillus reuteri in hypercholesterolemic mice. | swiss albino mice were fed a diet enriched with fat to produce hypercholesterolemia. the further administration of lactobacillus reuteri crl 1098 (10(4) cells/d) to hypercholesterolemic mice for 7 d decreased total cholesterol by 38%, producing serum cholesterol concentrations similar to that of the control group (67.4 mg/ml). this low dose of l. reuteri caused a 40% reduction in triglycerides and a 20% increase in the ratio of high density lipoprotein to low density lipoprotein without bacteria ... | 1998 | 9785223 |
| the effect of reuterin on listeria monocytogenes and escherichia coli o157:h7 in milk and cottage cheese. | a broad-spectrum reuterin produced during anaerobic fermentation of glycerol by lactobacillus reuteri strain 12002 was found to be inhibitory and bactericidal for listeria monocytogenes and escherichia coli o157:h7. lyophilized reuterin was prepared by a two-step fermentation process. a batch fermentation in a 15-liter fermentor was applied to produce a maximum biomass of l. reuteri using a modified mrs broth at ph 4.3. further, harvested cells were used to ferment glycerol (250 mm) under anaero ... | 1998 | 9798141 |
| safety and tolerance of lactobacillus reuteri supplementation to a population infected with the human immunodeficiency virus. | probiotic supplementation may provide health benefits, especially for individuals with an underlying disease state that makes them more susceptible to infections. the purpose of this experiment was to evaluate the safety and tolerance of lactobacillus reuteri ingestion by subjects infected with the human immunodeficiency virus (hiv). thirty-nine subjects consumed a freeze-dried preparation of l. reuteri or a placebo for 21 days in a double-masked, parallel design experiment. serum chemistry, hae ... | 1998 | 9862651 |
| application of reuterin produced by lactobacillus reuteri 12002 for meat decontamination and preservation. | lactobacillus reuteri strain 12002 was used for reuterin production in the two-step fermentation process. a batch culture fermentation was used to produce a maximum biomass of l. reuteri. then cells were harvested, resuspended in a glycerol-water solution, and anaerobically incubated to produce reuterin. the lyophilized supernatants (approximately 4000 activity units (au) of reuterin per ml) were diluted in distilled water for decontamination and preservation trials. the mic values of reuterin f ... | 1999 | 10090245 |
| the bspa locus of lactobacillus fermentum br11 encodes an l-cystine uptake system. | bspa is a basic surface-exposed protein from lactobacillus fermentum br11. sequence comparisons have shown that it is a member of family iii of the solute binding proteins. it is 89% identical to the collagen binding protein, cnb, from lactobacillus reuteri. compared with the database of escherichia coli proteins, bspa is most similar to the l-cystine binding protein fliy. to investigate the function of bspa, mutants depleted for bspa were generated by homologous recombination with a temperature ... | 1999 | 10094698 |
| lactobacillus species prevents colitis in interleukin 10 gene-deficient mice. | intestinal luminal microflora, or their products, are likely an important initiating factor in the pathogenesis of inflammatory bowel disease. the aim of this study was to determine the role of colonic aerobic luminal bacteria and lactobacillus species in the development of colitis in interleukin (il)-10 gene-deficient mice. | 1999 | 10220502 |
| autoaggregation of lactobacillus reuteri is mediated by a putative dead-box helicase. | we have cloned and sequenced a gene from lactobacillus reuteri that encodes a 56 kda protein, which mediates autoaggregation of the bacteria. using an antiserum raised against extracellular proteins from the pig intestinal isolate l. reuteri 1063, we screened a genomic lambda library derived from the same strain. affinity purification of recombinant protein from the isolated lambda clones showed that one type of clone expressed a protein that efficiently aggregated the parental strain when added ... | 1999 | 10231497 |
| biochemical and structural characterization of the glucan and fructan exopolysaccharides synthesized by the lactobacillus reuteri wild-type strain and by mutant strains | lactobacillus reuteri lb 121 cells growing on sucrose synthesize large amounts of a glucan (d-glucose) and a fructan (d-fructose) with molecular masses of 3,500 and 150 kda, respectively. methylation studies and 13c or 1h nuclear magnetic resonance analysis showed that the glucan has a unique structure consisting of terminal, 4-substituted, 6-substituted, and 4,6-disubstituted alpha-glucose in a molar ratio of 1.1:2.7:1.5:1.0. the fructan was identified as a (2-->6)-beta-d-fructofuranan or levan ... | 1999 | 10388696 |
| use of conserved randomly amplified polymorphic dna (rapd) fragments and rapd pattern for characterization of lactobacillus fermentum in ghanaian fermented maize dough. | the present work describes the use of randomly amplified polymorphic dna (rapd) for the characterization of 172 dominant lactobacillus isolates from present and previous studies of ghanaian maize fermentation. heterofermentative lactobacilli dominate the fermentation flora, since approximately 85% of the isolates belong to this group. cluster analysis of the rapd profiles obtained showed the presence of two main clusters. cluster 1 included lactobacillus fermentum, whereas cluster 2 comprised th ... | 1999 | 10388723 |
| cloning of erythromycin-resistance determinants and replication origins from indigenous plasmids of lactobacillus reuteri for potential use in construction of cloning vectors. | lactobacillus reuteri l1 and n16 strains contain a 7.0-kb plasmid (pte80) and a 15-kb plasmid (pte15), respectively, encoding resistance to erythromycin (em(r)). physical maps of both plasmids were established. nucleotide sequences of the genetic determinants encoding em(r) on pte80 and pte15 revealed the existence of a very similar (ca. 99% nucleotide sequence and ca. 98% amino acid sequence identity) open reading frame for an em(r) transmethylase gene (erm) in both plasmids. these structural e ... | 1999 | 10413663 |
| effects of lactobacillus reuteri on cryptosporidium parvum infection of gnotobiotic tcr-alpha-deficient mice. | | 1999 | 10519249 |
| supplementation with lactobacillus reuteri or l. acidophilus reduced intestinal shedding of cryptosporidium parvum oocysts in immunodeficient c57bl/6 mice. | the effect of l. acidophilus supplementation to reduce fecal shedding of cryptosporidium parvum oocysts was compared to l. reuteri using c57bl/6 female mice immunosuppressed by murine leukemia virus (strain lp-bm5) inoculation. after 12 weeks post lp-bm5 inoculation, 15 immunosuppressed mice each were randomly assinged to one of the following treatment groups: historical control (group a), lp-bm5 control (group b), c. parvum (group c), l. reuteri plus c. parvum (group d) or l. acidophilus plus c ... | 1999 | 10541481 |
| evidence of a glucose proton motive force-dependent permease and a fructose phosphoenolpyruvate:phosphotransferase transport system in lactobacillus reuteri crl 1098. | sugar uptake and phosphoenolpyruvate phosphorylation assays have shown that the heterofermentative strain lactobacillus reuteri crl 1098, of likely probiotic value, can transport d-fructose through an inducible fructose-specific phosphotransferase system (k(m) 95 microm) and d-glucose mainly through a proton motive force-driven permease. these data open new perspectives for metabolic and regulatory studies in this bacterium. | 1999 | 10564795 |
| molecular analysis of the microbial diversity present in the colonic wall, colonic lumen, and cecal lumen of a pig. | random clones of 16s ribosomal dna gene sequences were isolated after pcr amplification with eubacterial primers from total genomic dna recovered from samples of the colonic lumen, colonic wall, and cecal lumen from a pig. sequences were also obtained for cultures isolated anaerobically from the same colonic-wall sample. phylogenetic analysis showed that many sequences were related to those of lactobacillus or streptococcus spp. or fell into clusters ix, xiva, and xi of gram-positive bacteria. i ... | 1999 | 10583991 |
| influence of different functional elements of plasmid pgt232 on maintenance of recombinant plasmids in lactobacillus reuteri populations in vitro and in vivo. | plasmid pgt232 (5.1 kb), an indigenous plasmid of lactobacillus reuteri 100-23, was determined, on the basis of nucleotide and deduced protein sequence data, to belong to the pc194-pub110 family of plasmids that replicate via the rolling-circle mechanism. the minimal replicon of pgt232 was located on a 1.7-kb sequence consisting of a double-strand origin of replication and a gene encoding the replication initiation protein, repa. an erythromycin-selectable recombinant plasmid containing this min ... | 1999 | 10583992 |
| probiotics and infectious diarrhea. | numerous probiotic agents have been studied for the management of diarrheal disease. in particular, the prevention and management of acute viral diarrhea, the treatment of recurrent clostridium difficile diarrhea, as well as the control of antibiotic-associated diarrhea seem to be areas of significant potential benefit. a few agents, including lactobacillus gg, lactobacillus reuteri, and saccharomyces boulardii, seem to be promising agents for the amelioration of the course of acute diarrhea in ... | 2000 | 10634223 |
| purification and characterization of invertase from lactobacillus reuteri crl 1100. | the invertase of lactobacillus reuteri crl 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kda. the enzyme was stable below 45 degrees c over a wide ph range (4.5-7.0) with maximum activity at ph 6.0 and 37 degrees c. the invertase activity was significantly inhibited by bivalent metal ions (ca(++), cu(++), cd(++), and hg(++)), beta-mercaptoethanol, and dithiothreitol and partially improved by ethylenediaminetetraacetic acid. the enzyme was purified 32 times ove ... | 2000 | 10679050 |
| effect of lactobacillus reuteri on the prevention of hypercholesterolemia in mice. | administration of lactobacillus reuteri crl 1098 (10(4) cells/d) to mice for 7 d before inducing hypercholesterolemia (by feeding mice with a fat-enriched diet for the subsequent 7 d) was evaluated. at this low dose, l. reuteri was effective in preventing hypercholesterolemia in mice, producing a 17% increase in the ratio of high-density lipoprotein to low-density lipoprotein. total cholesterol and triglycerides decreased by 22 and 33%, respectively, in the group that was not fed the lactobacill ... | 2000 | 10750094 |
| multiplex pcr for the detection of lactobacillus pontis and two related species in a sourdough fermentation. | a specific multiplex pcr assay based on the amplification of parts of the 16s rrna molecule was designed. primers derived from variable regions of the 16s rrna provided a means of easily differentiating the species lactobacillus pontis and lactobacillus panis. they could be clearly discriminated from the phylogenetically related species lactobacillus vaginalis, lactobacillus oris, and lactobacillus reuteri and from other lactobacilli commonly known to be present in sourdough. other strains isola ... | 2000 | 10788389 |
| influence of culture ph on survival of lactobacillus reuteri subjected to freeze-drying. | l. reuteri was cultivated at ph 5 and 6. the cells were harvested at 0.5, 2.5 and 4.5 h after entering the stationary phase and their viability after freeze-drying was compared with their viability prior to freeze-drying. the highest viability--approximately 80% of the viability prior to freeze-drying--was obtained for cells from the ph 5 cultures harvested 2.5 h after entering the stationary phase. the time after entering the stationary phase did not influence the viability of the cells from th ... | 2000 | 10791749 |
| lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. | a new lactobacillus species from pig small intestine has been identified. in an attempt to isolate lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. a number of isolates were obtained and primary characterization showed that they were gram-positive, catalase-negative, non-spore-forming, non-motile rods. growth occurred at 45 degrees c but not at 15 degrees c and the dna g+c co ... | 2000 | 10826811 |
| rapid identification of 11 human intestinal lactobacillus species by multiplex pcr assays using group- and species-specific primers derived from the 16s-23s rrna intergenic spacer region and its flanking 23s rrna. | rapid and reliable two-step multiplex polymerase chain reaction (pcr) assays were established to identify human intestinal lactobacilli; a multiplex pcr was used for grouping of lactobacilli with a mixture of group-specific primers followed by four multiplex pcr assays with four sorts of species-specific primer mixtures for identification at the species level. primers used were designed from nucleotide sequences of the 16s-23s rrna intergenic spacer region and its flanking 23s rrna gene of membe ... | 2000 | 10856652 |
| protective effect of the bile salt hydrolase-active lactobacillus reuteri against bile salt cytotoxicity. | bacterial bile salt hydrolysis is considered a risk factor for the development of colon cancer because of the risk of forming harmful secondary bile salts after an initial deconjugation step. in this study, the influence of enhanced bacterial bile salt transformation by the bile salt hydrolase-active lactobacillus reuteri was studied in batch culture using the microbial suspension of the simulator of the human intestinal microbial ecosystem; (shime), which was supplemented with oxgall at 5 g/l o ... | 2000 | 10919331 |
| purification and characterization of a surface-binding protein from lactobacillus fermentum rc-14 that inhibits adhesion of enterococcus faecalis 1131. | lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. one strain, lactobacillus fermentum rc-14, releases surface-active components which can inhibit adhesion of uropathogenic bacteria. using a quantitative method for determining inhibition of adhesion, a protein with high anti-adhesive properties against enterococcus faecalis 1131 was purified. the n-terminal sequence of the 29-kda protein was identical to that of a collagen-binding protein from lactob ... | 2000 | 10981710 |
| characterization of reutericyclin produced by lactobacillus reuteri lth2584. | lactobacillus reuteri lth2584 exhibits antimicrobial activity that can be attributed neither to bacteriocins nor to the production of reuterin or organic acids. we have purified the active compound, named reutericyclin, to homogeneity and characterized its antimicrobial activity. reutericyclin exhibited a broad inhibitory spectrum including lactobacillus spp., bacillus subtilis, b. cereus, enterococcus faecalis, staphylococcus aureus, and listeria innocua. it did not affect the growth of gram-ne ... | 2000 | 11010877 |
| denaturing gradient gel electrophoresis analysis of 16s ribosomal dna amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of lactobacillus reuteri strain mm53. | the diversity and stability of the fecal bacterial microbiota in weaning pigs was studied after introduction of an exogenous lactobacillus reuteri strain, mm53, using a combination of cultivation and techniques based on genes encoding 16s rrna (16s rdna). piglets (n = 9) were assigned to three treatment groups (control, daily dosed, and 4th-day dosed), and fresh fecal samples were collected daily. dosed animals received 2.5 x 10(10) cfu of antibiotic-resistant l. reuteri mm53 daily or every 4th ... | 2000 | 11055913 |
| improved growth and viability of lactobacilli in the presence of bacillus subtilis (natto), catalase, or subtilisin. | in an effort to demonstrate the potential usefulness of bacillus subtilis (natto) as a probiotic, we examined the effect of this organism on the growth of three strains of lactobacilli co-cultured aerobically in vitro. addition of b. subtilis (natto) to the culture medium resulted in an increase in the number of viable cells of all lactobacilli tested. since b. subtilis (natto) can produce catalase, which has been reported to exhibit a similar growth-promoting effect on lactobacilli, we also exa ... | 2000 | 11068675 |
| molecular analysis of mutated lactobacillus acidophilus promoter-like sequence p15. | the promoter-like sequence p15 that was previously cloned from the chromosome of lactobacillus acidophilus atcc 4356 is active in lactobacillus reuteri, lactobacillus plantarum, lactobacillus acidophilus, and escherichia coli, but not in lactococcus lactis. n-methyl-n-nitroso-n-guanidine (mnng) mutagenesis of p15 was used to select for a promoter active in l. lactis mg1363. molecular analysis of the mutated promoter (designated p16) revealed a 90 bp deletion and a t-->a transversion. this deleti ... | 2000 | 11068681 |
| kinetics characterization of taurocholic transport in lactobacillus reuteri. | taurocholic acid transport in lactobacillus reuteri crl 1098 was determined. the bile acid is incorporated inside the cells by an active and saturable transport showing a typical kinetics of michaelis-menten with values of km and vmax of 0.35 mm and 20 mm, respectively. | 2001 | 11116391 |
| exclusion of vana, vanb and vanc type glycopeptide resistance in strains of lactobacillus reuteri and lactobacillus rhamnosus used as probiotics by polymerase chain reaction and hybridization methods. | strains of lactobacillus reuteri and lact. rhamnosus are used as probiotics in man and animal. the aim of this study was to determine whether the glycopeptide resistance in these lactobacilli has a similar genetic basis as in enterococci. five lact. reuteri strains and one lact. rhamnosus, as well as four enterococcus control strains, were probed for the vana gene cluster, the vanb gene and the vanc gene by pcr and southern hybridization, and dna/dna hybridization. their resistance and plasmid p ... | 2000 | 11119156 |
| lactobacillus frumenti sp. nov., a new lactic acid bacterium isolated from rye-bran fermentations with a long fermentation period. | within the framework of the characterization of the microflora of an industrial sourdough fermentation, strains of lactobacillus amylovorus, lactobacillus pontis and two other strains were isolated which could not be associated with a valid species. these latter strains were gram-positive, catalase-negative, non-spore-forming, non-motile rods that could be clearly differentiated from known species by 16s rdna sequence analysis. for further characterization, the morphological, physiological (suga ... | 2000 | 11155988 |
| a comparative study on the use of flow cytometry and colony forming units for assessment of the antibacterial effect of bacteriocins. | flow cytometry was investigated as a rapid method to determine the antibacterial effect of the bacteriocins nisin, pediocin pa-1, and sakacin a on the indicator organisms lactobacillus reuteri dsm 12246, lactobacillus sakei ncfb 2714 and lactobacillus sakei dsm 20017, respectively. fluorescence intensities of the cells were measured by flow cytometry upon exposure to bacteriocins after staining with carboxyfluorescein diacetate (cfda) and were compared to the number of colony forming units (cfu) ... | 2001 | 11205955 |
| sequence analyses of a broad host-range plasmid containing ermt from a tylosin-resistant lactobacillus sp. isolated from swine feces. | anaerobic bacteria resistant to the macrolide antibiotics tylosin and erythromycin were isolated from the feces of swine. one of the strains, 121b, was initially identified by 16s rdna sequence analysis as an unknown lactobacillus sp. the strain was found to contain at least two plasmids, one of which was capable of replicating and providing erythromycin and tylosin resistance to bacillus subtilis, streptococcus gordonii, and escherichia coli. dna sequence analyses of the 4,232-bp plasmid, p121b ... | 2001 | 11375658 |
| effects of lactobacillus rhamnosus gg and lactobacillus reuteri r2lc on acetic acid-induced colitis in rats. | certain lactobacilli reduce the severity of experimental colitis. the aim of this study was to compare the effects of a human strain lactobacillus rhamnosus gg and a rat strain lactobacillus reuteri r2lc on acetic acid-induced colitis in rats. | 2001 | 11424322 |
| amino acid catabolism in cheese-related bacteria: selection and study of the effects of ph, temperature and nacl by quadratic response surface methodology. | to screen the cystathionine lyase and l-methionine aminotransferase activities of cheese-related bacteria (lactococci, non-starter lactobacilli and smear bacteria) and to determine the individual and interactive effects of temperature, ph and nacl concentration on selected enzyme activities. | 2001 | 11473596 |
| characterization of the replication region of the lactobacillus reuteri plasmid ptc82 potentially used in the construction of cloning vector. | a 3.2 kb dna fragment containing the replication region (rr) from ptc82 was cloned, sequenced, and found to contain elements typical of plasmids that replicate via a rolling-circle mechanism of replication (rcr), including double-strand origin (dso), replication protein gene (rep), and single-strand origin (sso). the dso of ptc82 contains two domains showing 55.5% and 84.6% similarities in nucleotide (nt) sequence to the conserved functional elements bind and nic, respectively, which are require ... | 2001 | 11515531 |
| addition of mucin to the growth medium triggers mucus-binding activity in different strains of lactobacillus reuteri in vitro. | we have examined the ability of a number of lactobacillus reuteri strains to bind immobilised mucus material. after growth in mrs broth, some strains showed high binding activity towards mucus whilst many strains exhibited a very low binding activity. in order to simulate the intestinal milieu, we grew the bacteria in mrs supplemented with the glycoprotein mucin, the main component of mucus. growth under these conditions dramatically improved the mucus-binding activity of most strains that initi ... | 2001 | 11682171 |
| influence of adjunct cultures on ripening of reduced fat edam cheeses. | the influence of four adjunct cultures [brevibacterium linens (bl2), lactococcus lactis ssp. diacetylactis, lactobacillus helveticus (lh212), and lactobacillus reuteri (atcc 23272)] on chemical and sensory characteristics of reduced fat edam cheese was studied. the aminopeptidase activity of lactococcus lactis ssp. diacetylactis was higher than that of lactobacillus helveticus, lactobacillus reuteri, and brevibacterium linens, respectively. mean percent fat and moisture contents of reduced fat c ... | 2001 | 11699441 |
| high prevalence of inducible erythromycin resistance among streptococcus bovis isolates in taiwan. | susceptibilities to 13 antimicrobial agents were determined by measurement of mics for 60 isolates of streptococcus bovis from blood cultures. thirty-eight isolates (63.3%) had high-level resistance to erythromycin (mics, >or=128 microg/ml). among the 38 erythromycin-resistant strains, 21 isolates (55%) had inducible resistance to macrolides-lincosamides-streptogramin b (imls isolates) and 17 (45%) had constitutive resistance to macrolides-lincosamides-streptogramin b (cmls isolates). tetracycli ... | 2001 | 11709309 |
| combined use of lactobacillus reuteri and soygerm powder as food supplement. | the survival of lactobacillus reuteri when challenged with glycodeoxycholic acid (gdca), deoxycholic acid (dca) and soygerm powder was investigated. moreover, the impact of lact. reuteri on the bioavailability of isoflavones present in soygerm powder was examined. | 2001 | 11737624 |
| purification of a novel fructosyltransferase from lactobacillus reuteri strain 121 and characterization of the levan produced. | fructosyltransferase (ftf) enzymes have been characterized from various gram-positive bacteria, but not from lactobacillus sp. in a screening of 182 lactobacilli for polysaccharide production only one strain, lactobacillus reuteri strain 121, was found to produce a fructan being a levan. here we report the first-time identification and biochemical characterization of a lactobacillus ftf enzyme. when incubated with sucrose the enzyme produced a levan that is identical to that produced by lb. reut ... | 2001 | 11750822 |
| lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. | dendritic cells (dc) play a pivotal immunoregulatory role in the th1, th2, and th3 cell balance and are present throughout the gastrointestinal tract. thus, dc may be targets for modulation by gut microbes, including ingested probiotics. in the present study, we tested the hypothesis that species of lactobacillus, important members of the gut flora, differentially activate dc. bone marrow-derived murine dc were exposed to various lethally irradiated lactobacillus spp. and resultant culture super ... | 2002 | 11751960 |
| the peptide hydrolase system of lactobacillus reuteri. | peptide hydrolase system of lactobacillus reuteri crl 1098, a lactic acid bacteria of sourdough origin, was investigated. this microorganism has a broad range of peptidases consisting of an active aminopeptidase, x-prolyl-dipeptidylaminopeptidase, dipeptidase and tripeptidase. aminopeptidase, iminopeptidase and endopeptidase are most likely located in the cytoplasmic fraction showing no detectable association with the cell membrane, while dipeptidase and tripeptidase are mainly associated with t ... | 2001 | 11764195 |
| inhibition of binding of helicobacter pylori to the glycolipid receptors by probiotic lactobacillus reuteri. | we examined the competition of binding of lactobacillus reuteri and helicobacter pylori to gangliotetraosylceramide (asialo-gm1) and sulfatide which are putative glycolipid receptor molecules of h. pylori, and identified a possible sulfatide-binding protein of the l. reuteri strain. among nine l. reuteri strains, two (jcm1081 and tm105) were shown to bind to asialo-gm1 and sulfatide, and to inhibit binding of h. pylori to both glycolipids by a thin layer chromatogram-overlay assay using biotin-l ... | 2002 | 11821231 |
| a high-molecular-mass cell-surface protein from lactobacillus reuteri 1063 adheres to mucus components. | a gene from lactobacillus reuteri 1063 encoding a cell-surface protein, designated mub, that adheres to mucus components in vitro has been cloned and sequenced. the deduced amino acid sequence of mub (358 kda) shows the presence of 14 approximately 200 aa repeats and features typical for other cell-surface proteins of gram-positive bacteria. fusion proteins consisting of different repeats of mub and the maltose-binding protein (mbp) were produced. these proteins adhered to pig mucus components, ... | 2002 | 11832507 |
| characterization of an alanine racemase gene from lactobacillus reuteri. | an alanine racemase gene from lb. reuteri was cloned by using degenerate oligonucleotides corresponding to conserved regions derived from several bacterial alanine racemases. the protein is 375alphaalpha in length and shows 63.6% homology to the lb. plantarum alanine racemase. unlike the single alanine racemase activity found in lb. plantarum, deletion of the lb. reuteri alanine racemase reveals a second activity, which is inhibited by beta-chloro- d-alanine. | 2002 | 11910493 |
| characterisation and modelling of oscillatory behaviour related to reuterin production by lactobacillus reuteri. | during reuterin production by lactobacillus reuteri in a chemostat, the growth, substrate and metabolite concentrations showed oscillatory behaviour. the sensitivity of l. reuteri towards reuterin was shown to be a possible explanation of the oscillatory behaviour. a deterministic mathematical model consisting of four coupled differential equations describing the concentrations of biomass, glucose, glycerol and reuterin with time was developed. with a set of parameter values determined from batc ... | 2002 | 11934046 |
| lactobacillus durianis sp. nov., isolated from an acid-fermented condiment (tempoyak) in malaysia. | lactic acid bacteria (lab) are the predominant micro-organisms in tempoyak, a malaysian acid-fermented condiment. in a study on the diversity of lab in this product, three isolates could not be identified using sds-page of whole-cell proteins or api 50 ch. the taxonomic position of the three isolates was clarified in the present study. 16s rdna sequencing classified a representative strain in the genus lactobacillus, clearly separated from all known species, and most closely related to the lacto ... | 2002 | 12054259 |
| biocompatibility study of biological tissues fixed by a natural compound (reuterin) produced by lactobacillus reuteri. | glutaraldehyde-fixed biological tissues have been used extensively to fabricate various bioprostheses. however, the tendency for glutaraldehyde to markedly alter tissue stiffness and promote tissue calcification is a well-recognized drawback of this crosslinking agent. to overcome the aforementioned deficiency with the glutaraldehyde-fixed bioprostheses, a fixation technique using a natural compound (reuterin) produced by lactobacillus reuteri to crosslink biological tissues was developed by our ... | 2002 | 12102192 |
| effect of glucose on glycerol bioconversion by lactobacillus reuteri. | the impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-hpa) accumulation by resting cells of lactobacillus reuteri has been investigated. two systems were used in the study: mrs(-) (modified mrs - omitting glucose, acetate and tween 80) and distilled water (h(2)o). in mrs(-), addition of glucose enhanced glycerol metabolism in resting cells of l. reuteri, consequently increasing the accumulation of 3-hpa by regulating the nad/nadh ratio. enhanced glycerol metabol ... | 2002 | 12111160 |
| feasibility study using a natural compound (reuterin) produced by lactobacillus reuteri in sterilizing and crosslinking biological tissues. | bioprostheses derived from biological tissues have to be fixed and subsequently sterilized before they can be implanted in humans. currently available crosslinking agents and sterilants used in the fixation or sterilization of biological tissues such as glutaraldehyde and formaldehyde are all highly cytotoxic, which may impair the biocompatibility of bioprostheses. therefore, it is desirable to provide an agent suitable for use in biomedical applications that is of low cytotoxicity and may form ... | 2002 | 12115461 |
| effect of probiotic lactobacillus strains in young children hospitalized with acute diarrhea. | oral bacteriotherapy promotes recovery from acute childhood diarrhea, but few strains have been shown to have therapeutic potentials. we examined the effect of two newly identified probiotic lactobacillus strains in acute childhood diarrhea. | 2002 | 12150178 |