characterization of an extracellular factor that stimulates bile salt hydrolase activity in lactobacillus sp. strain 100-100.bile salt hydrolase activity in lactobacillus sp. strain 100-100 is strictly intracellular. the strain produces an extracellular factor that stimulates the intracellular hydrolase activity. the factor is inducible by conjugated bile salts, has an apparent molecular mass over 12 kda but less than 25 kda, is stable in air, and resistant to pronase and heat. it is partially extractable into organic solvents and inactivated by a sulphydryl group inhibitor. we postulate that the factor functions by a ...19921521759
the role of intestinal microflora on the prevention of salmonella colonization in gnotobiotic chickens.the possible role of the intestinal microflora in inhibiting the colonization of salmonella in the ceca of gnotobiotic chickens was investigated. in the cecal contents of newly hatched (2-day-old) and adult normal (7-mo-old) chickens, a larger number of escherichia coli and a smaller number of lactobacillus sp. were noticed in 2-day-old chickens than in adult chickens. gnotobiotic chickens treated with cecal contents from either newly hatched or adult birds were orally exposed to salmonella typh ...19911780261
the effects of temperature and growth rate on the proportion of unsaturated fatty acids in bacterial lipids.the effects of temperature and growth rate on the fatty acid composition of the extractable lipids of four mesophilic and three psychotrophic bacteria were examined. two of the mesophiles (escherichia coli and pseudomonas aeruginosa) increased the proportion of unsaturated fatty acids in their lipids with decreasing temperature over their whole growth temperature range. the other mesophiles (enterobacter aerogenes and lactobacillus casei) increased the proportion of unsaturated fatty acids with ...1978624935
[significance of the bacterial flora in the etiology of apical periodontitis. qualitative, quantitative and topographical aspects].in the international literature it is shown the central role of root canal infection in the etiology of periapical lesions. as a matter of fact it has been proved (13) that sterile necrotic pulp tissue is completely unable to cause inflammatory reactions at the periapex. infection of endodontic origin extends to the supporting tissues of the tooth only in the case of their acute inflammation (e.g. acute apical periodontitis, acute alveolar abscess, phoenix abscess). on the other hand in chronic ...19902135040
infection and labor. vii. microbial invasion of the amniotic cavity in spontaneous rupture of membranes at term.the purpose of this study was to determine the frequency, microbiologic characteristics, and clinical significance of microbial invasion of the amniotic cavity in women with premature rupture of membranes at term.19921301006
lactobacillus sp. isolates from mouse livers: saprophyte or potential pathogen?over a 3-year sampling period lactobacillus sp. has been isolated from the livers of barrier-maintained and conventional mice. serology has failed to detect presence of mouse hepatitis virus antibodies in any of these mice. histopathological examination revealed only mild fatty change and small monocytic infiltrates.1975806745
production of an antibiotic substance by bacillus licheniformis within the digestive tract of gnotobiotic monoxenic mice, vegetative cells and spores of bacillus licheniformis were enumerated and in vivo antibiotic production was measured at various levels of the digestive tract and in the feces. the apparent independence of vegetative cell and spore populations in the cecum and feces, as well as the marked fluctuations of these two populations in the stomach and small intestine, suggested that sporulation of b. licheniformis and production of antibiotic occur only in the upper levels of the dige ...1978626497
bacteremia after upper gastrointestinal endoscopy.during 24 months, 200 upper gastrointestinal endoscopies were performed on 193 patients. blood cultures were obtained before and five and 30 minutes after the procedure using thiol (50 ml) and trypticase soy broth (100 ml) media. the mean endoscopic time was 34 minutes. sixteen patients developed bacteremia (8%). twelve groups of microorganisms were detected in positive blood cultures: streptococcus (5 species), lactobacillus sp, veillonella alcalescens, staphylococcus aureus, staph epidermidis, ...1977404974
microbiological characteristics of dungeness crab (cancer magister).aerobic, heterotropic microorganisms of dungeness crab (cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. each microbial isolate was then identified to the genus level employing the revised replica plating procedure. microbial groups most commonly isolated from crab meat were, in the order of predominance, moraxella, pseudomonas, acinetobacter, arthrobacter, micrococcus, flavobacterium-cytophaga, and ba ...19751096824
bacterial the western world, bacterial vaginosis is now being recognised as the commonest cause of vaginitis. bacterial vaginosis represents a major disturbance in vaginal microbiology with the massive overgrowth of predominantly anaerobic bacteria normally found in the vagina. accordingly, high population levels of non-fragilis bacteroides sp., peptostreptococci, gardnerella vaginalis and mobiluncus sp. are found, with a corresponding decline in lactobacillus sp. excessive polyamine production by anae ...19902091736
isolation and characteristics of a skatole-producing lactobacillus sp. from the bovine rumen.a bacterium that is capable of decarboxylating indoleacetic acid to skatole (3-methylindole) has been isolated from an l-tryptophan enrichment of bovine rumen fluid. the bacterium is a gram-positive, nonmotile, nonsporeforming rod. it is an obligate anaerobe, and strains predominatly produce d-(-)-lactic acid, with smaller amounts of l-(+)-lactic acid and acetic acid, from sugar. all four strains isolated gave a negative reaction to the indole test because they cannot form skatole directly from ...1977563703
influence of certain indigenous gastrointestinal microorganisms on duodenal alkaline phosphatase in mice.alkaline phosphatase activity was assayed in duodenal homogenates or extracts from adult specific pathogen-free (spf) and germfree mice and gnotobiotic mice monoassociated with a lactobacillus sp., a bacteroides sp., or a coliform strain indigenous to spf mice. activity levels of the enzyme were much higher in the preparations from germfree mice than in those from the spf controls. in the gnotobiotes monoassociated either with a freshly isolated lactobacillus sp. or a bacteroides sp., the levels ...1976779647
characterization of plasmids and plasmid-borne macrolide resistance from lactobacillus sp. strain 100-33.lactobacillus sp. strain 100-33 is resistant to macrolides, lincosamides, and streptogramin b-type antibiotics (mlsr) and appears to contain several major and minor plasmids. one of these plasmids, plar33, is approximately 18 kbp in size. when cells of strain 100-33 were protoplasted and regenerated, an mlss isolate was derived. the derivative, designated strain es1, contained a unique plasmid complement in which it had apparently lost the major plasmids of the parental strain, including plar33, ...19902163536
some taxonomical characteristics of encapsulated lactobacillus sp. kpb-167b isolated from kefir grains and characterization of its extracellular polysaccharide.a capsular polysaccharide-producing strain kpb-167b isolated from kefir grains was identified as a homofermentative lactobacillus. the carbohydrate fermentation pattern and dna base composition of the strain were different from those of other capsular lactobacillus species previously isolated from kefir grains. the polysaccharide produced by lactobacillus sp. kpb-167b was found similar to kefiran by 13c-nmr and methylation analysis. lactobacillus sp. kpb-167b could grow and produce capsular poly ...19911911082
bacterial flora of the vagina and uterus of healthy cats.bacterial culturing was conducted on samples from the reproductive tracts of 53 clinically healthy female cats. aerobic bacteria were isolated from 52 of 53 vaginal swab samples and from 2 of 29 uterine swab samples. anaerobic bacteria were detected in 4 of 30 vaginal and 1 of 29 uterine cultures. the aerobic bacteria included species of acinetobacter, actinomyces, corynebacterium, escherichia, haemophilus, klebsiella, lactobacillus, staphylococcus, and streptococcus. coagulase-negative staphylo ...19902179182
lactobacillus casei subspecies casei endocarditis--a case report.lactobacillus sp., generally considered to be a harmless indigenous bacteria of the mucous membrane, occasionally causes serious infections. lactobacillus endocarditis is a very rare disease, and no case has been reported in korea. gram-positive bacilli were isolated from blood cultures of a 41-year-old man with clinically suspected subacute bacterial endocarditis. the patient had a dental procedure 3 months prior to the infection. the isolate was identified as l. casei subsp. casei based on the ...19911908610
construction of a new shuttle vector for clone the malolactic enzyme gene from lactobacillus sp. 89, construction of a shuttle vector able to express itself in lactobacillus sp. 89 and escherichia coli was undertaken. the shuttle plasmid ple16 resulted from the union of pbr328 and of the plb10 plasmid extracted from lactobacillus bulgaricus 10. the bacterial transformation in lactobacillus sp. 89 was performed by electroporation, and the clones were selected on mrs medium with 30 chloramphenicol added. fifty percent ...19921581865
[surgical treatment of acute bacteroid purulent cholangitis].the results of treatment of 40 patients with acute purulent cholangitis developed against the background of choledocholithiasis are presented. bacteroid of the kind b. fragilis (67.5% of cases), fusobacterium, lactobacillus sp. (30%) are the leading etiological factor of cholangitis. the indications for external and internal drainage of the bile ducts are substantiated. the ineffectiveness of internal drainage in this disease is demonstrated.19911766182
[plasmid pbs195 with a wide range of hosts, isolated from lactobacilli].the nonconjugative 4.4 kb plasmid pbs195 has been found in lactobacillus sp. 195 strain resistant to kanamycin and streptomycin. the plasmid pbs195 determining the resistance to kanamycin has a broad host range. it is inherited by the gram-positive microorganisms (bacillus subtilis) as well as by escherichia coli cells, has the cleavage sites for the restriction endonucleases bamhi, ecori, hindiii, psti, kpni. the restriction map of the plasmid for these enzymes is constructed. the broad host ra ...19911896058
enhancement of the circulating antibody secreting cell response in human diarrhea by a human lactobacillus strain.human lactobacillus sp strain gg (lactobacillus gg) administered during acute rotavirus diarrhea has been shown to promote clinical recovery. to elucidate the immune mechanisms behind such a favorable outcome, the elispot (solid phase enzyme-linked immunospot) assay of ig- and specific antibody-secreting cells among circulating lymphocytes was used, giving indirect evidence of the immunologic events in the gut. after rehydration, 39 children with acute rotavirus diarrhea, mean age 16 (sd 6) mo, ...19921324462
the role of bacterial vaginosis as a cause of amniotic fluid infection, chorioamnionitis and prematurity--a review.antepartum bacterial vaginosis in pregnancy has been related to premature delivery, the recovery of microorganisms from amniotic fluid of women in premature labor with intact membranes, to histologic chorioamnionitis and to the recovery of microorganisms from the placenta or membranes. microorganisms associated with bacterial vaginosis are commonly recovered from the amniotic fluid and chorioamnion of patients who deliver prematurely. in addition, bacterial vaginosis is associated with maternal ...19902178562
digestive tract microflora liberates low molecular weight peptides with immunotriggering activity.antibiotic digestive tract decontamination in balb/c-mice resulted in a significant reduction of peritoneal macrophage function and lymphocyte proliferation. considerable evidence has accumulated showing that certain species of the indigenous gastrointestinal (gi)-tract microflora, e.g. bacteroides sp., clostridium sp., lactobacillus sp., and propionibacterium sp., liberate low molecular weight peptides which are able to trigger basic immune responses. eradication of the gi-tract microflora appa ...19902331298
multiple forms of bile salt hydrolase from lactobacillus sp. strain 100-100.four isozymes of bile salt hydrolase (bsh) have been purified from the cytosol of cells of lactobacillus sp. strain 100-100. the four proteins were designated bsh a, b, c, and d. they eluted from anion-exchange high-pressure liquid chromatography columns at 0.15, 0.18, 0.21, and 0.25 m nacl, respectively. they are catalytically similar, except that the vmax of bsh d is about 10-fold lower than those of the other three isozymes. all four proteins consist of one or two polypeptides. the peptides h ...19921429446
[comparative study of the bacterial flora in both uterocervical and uterine regions].the relationship between indigenous bacterial floras in the lower genitals and the uterocervical region is of importance due to a barrier-like role against ascending infections through the mechanism of the localized protection at a boundary of the uterocervical region. therefore, bacterial flora in the uterocervical and uterine regions was studied in cases for which gynecological operations were performed (n = 77). 1. ratios of incidences of the detection of bacteria on the uterocervical and in ...19902362354
introduction to mechanisms of association of indigenous microbes.indigenous microorganisms of numerous types associate with epithelial surfaces in the gastrointestinal tracts of mammals and birds. some of the microbial types, e.g., lactobacillus sp. in the stomachs of laboratory rodents, adhere to a surface without altering it ultrastructurely. in such cases, the adherence is mediated undoubtedly by macromolecules on the bacterial surfaces, possibly polysaccharides in most cases, interacting in specific ways with receptor macromolecules on the epithelial surf ...1979367138
review article: prevention and treatment of travellers' diarrhoea.travellers' diarrhoea is a common problem worldwide and is likely to continue to increase with the expansion of foreign travel. etec is the most frequently isolated enteropathogen although other bacteria, parasites and viruses may be important, depending on the geographic location. new pathogens may well emerge in the future. prevention must be the ultimate goal but while awaiting effective vaccines we must be content with the traditional dietary advice and its often unpalatable and unacceptable ...19911932478
[candida krusei in genital infections].frequency of appearance of candida krusei in human reproductive tract, susceptibility of these microorganisms to drugs and their importance in pathogenesis of mycosis, were investigated. strains c. krusei species constituted 6.7% of the total 1325 strains and 21.8% within other than c. albicans species yeast-like fungi. vaginal mycosis caused by fungi from c. krusei species occurs in presence of lactobacillus sp. and correct values of ph in vaginal contents, however changed leukocytosis in evide ...19921297039
[the role of candida kefyr (c. pseudotropicalis) in reproductive tract infections].frequency of appearance of c. kefyr strains, their diagnosis, susceptibility to drugs and importance in pathogenesis of reproductive tract mycoses were investigated. the investigated material consisted of 2717 strains, and there were 67 strains of c. kefyr isolated from vaginal mucous membrane. c. kefyr strains constituted 2.5% of total number of 2717 isolated strains and 8.2% within fungi other than c. albicans species. vaginal mycosis caused by c. kefyr occurs in presence of lactobacillus sp. ...19921297040
lactobacillus hilgardii plasmid plab1000 consists of two functional cassettes commonly found in other gram-positive organisms.a lactobacillus hilgardii plasmid, plab1000, was studied to understand the organization of autonomous replicons from lactobacilli. two cassettes could be identified. first, the replication region consisted of a sequence coding for a replication protein (rep) and its corresponding target site, similar to those from plasmids pub110, pc194 (staphylococcus aureus), pftb14, pbaa1 (bacillus sp.), and plp1 (lactobacillus sp.). sequence analysis indicated the possible synthesis of an antisense rna that ...19902188951
characterization and purification of bile salt hydrolase from lactobacillus sp. strain 100-100.we have characterized and purified the bile salt hydrolase from lactobacillus sp. strain 100-100. bile salt hydrolase from cells of the strain was purified with column and high-performance liquid chromatography. the activity was assayed in whole cells and cell-free extracts with either a radiochemical assay involving [14c]taurocholic acid or a nonradioactive assay involving trinitrobenzene sulfonate. the activity was detectable only in stationary-phase cells. within 20 min after conjugated bile ...19902376558
role of erythrosine in the inhibition of adhesion of lactobacillus fermentum strain 737 to mouse stomach tissue.the mechanism by which the food colour erythrosine inhibits the adhesion of lactobacillus sp. to squamous epithelium in the mouse stomach was investigated using an in vitro adhesion assay. inhibition of adhesion occurred only after growth of l. fermentum in erythrosine which bound to the bacterial cell surface. erythrosine did not interfere with the receptor on the epithelial cell surface. growth, but not the atp content per cell, was affected by the presence of erythrosine in the growth medium. ...19892559943
assay for the enzymatic conversion of indoleacetic acid to 3-methylindole in a ruminal lactobacillus assay to measure the rate of enzymatic formation of 3-methylindole (3mi) from indoleacetic acid (iaa) in lactobacillus sp. strain 11201 was developed. the reaction mixture contained 50 micrograms of microbial protein per ml (range, 25 to 100 mg/ml), essential low-molecular-weight reaction ingredients, and radiolabeled iaa as substrate (range, 0 to 2 mm iaa). the reaction was anaerobic for 25 min at 39 degrees c. the apparent michaelis-menten constants were: km, 0.14 mm iaa; and vmax, 64 nmol ...19902317043
relationship of bacteriologic characteristics to semen indices in men attending an infertility clinic.bacteria can be isolated from most seminal fluid samples, but the significance of these microorganisms is uncertain because most men lack symptoms associated with bacterial infection of the reproductive tract. we obtained semen samples from 37 men attending a special infertility clinic and assessed the relationship between seminal fluid microorganisms and seminal fluid analysis including sperm motility, morphology, and concentration; the numbers of polymorphonuclear leukocytes and other white bl ...19902325963
[occurrence and the sensitivity to drugs of fungi other than candida albicans isolated from the vagina].frequency of occurrence of fungal species distinct from c. albicans isolated from vaginal mucosa and their sensitivity to antimycotic chemotherapeutics was determined. material consisted of 452 fungal strains isolated from vagina from patients suffering from afflictions within genital area. fungal strains isolated belonged to 13 genera. fungi distinct from c. albicans constituted 27.1% of all the strains. fungi the most frequently isolated from vagina belonged to following genera: t. glabrata 35 ...19892668661
chemical, microbial and sensory changes during the anaerobic cold storage of beef inoculated with a homofermentative lactobacillus sp. or a leuconostoc sp.slices of beef were inoculated with about 3.5 log cfu/cm2 of lactobacillus sp. 93 smricc 235 (homofermentative) or leuconostoc sp. 89 smricc 189 and stored in 5% co2 + 95% n2 at 4 degrees c. the microbial, chemical (glucose, l-lactate, d-lactate, acetate, formate, ethanol, h2s) and sensory changes of the beef slices were studied. for beef inoculated with lactobacillus sp. 93 the flavour score started to decrease when the maximum bacterial count was reached. leuconostoc sp. 89 caused a rapid decr ...19921622763
survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells.the survival of four strains of lactic acid bacteria in human gastric juice, in vivo and in vitro, and in buffered saline, ph 1 to 5, has been investigated. the strains studied include two lactobacillus acidophilus strains, lactobacillus bulgaricus, and streptococcus thermophilus. in addition, the adhesion of these strains to freshly collected human and pig small intestinal cells and to pig large intestinal cells has been studied and the effect of milk on both survival and adhesion tested. as a ...19873106442
new method to study bacterial adhesion to meat.a new method was developed for the study of bacterial adhesion to meat surfaces. thin slices of meat (40 microns thick) were inserted into a specially designed observation chamber. the meat slices were then exposed to a bacterial suspension (ca. 10(6) to initiate adhesion (20 min of contact time) and subsequently rinsed to eliminate nonadherent bacteria. because of the special chamber design, the disruptive force exerted on the bacteria during rinsing (shear stress) was uniform over th ...19892764565
susceptibility and resistance of ruminal bacteria to antimicrobial feed additives.susceptibility and resistance of ruminal bacterial species to avoparcin, narasin, salinomycin, thiopeptin, tylosin, virginiamycin, and two new ionophore antibiotics, ro22-6924/004 and ro21-6447/009, were determined. generally, antimicrobial compounds were inhibitory to gram-positive bacteria and those bacteria that have gram-positive-like cell wall structure. mics ranged from 0.09 to 24.0 micrograms/ml. gram-negative bacteria were resistant at the highest concentration tested (48.0 micrograms/ml ...19873116929
[etiology of infectious endocarditis at the instituto nacional de cardiologia ignacio chavez from 1976 to 1986].we studied 85 cases of infectious endocarditis (ie) with an anatomical diagnosis (biopsy or necropsy). most cases occur between the second and fourth decade of life; male/female ratio was 1/0.8; 45 had ie in a natural valve; 40 had prosthetic ie. clinical diagnosis was established or at least suspected in 61 cases (72%); the most common misdiagnosis was acute rheumatic fever. blood cultures were positive in 25 cases of natural valve ie (56%) and in 25 cases of prosthetic ie (63%). half of those ...19892782992
[diagnostic and clinical characteristics of mycotic vaginitis caused by candida glabrata (torulopsis glabrata)].in this study an evaluation of frequency of occurrence of c. glabrata, its diagnosis, sensitivity to antifungal drugs and its significance in pathogenesis of mycotic vaginosis was performed. strains belonging to c. glabrata genus constituted 12.1% of total of 852 isolated strains and 39.2% of strains other than c. albicans. during fungal vaginosis caused by c. glabrata lactobacillus sp. was present and normal ph values of vaginal secretion were seen. in direct preparations single or few leukocyt ...19892701236
evidence for the conjugal transfer of the broad host range plasmid pip501 into strains of lactobacillus helveticus.the conjugative broad host range plasmid pip501 was transferred from streptococcus faecalis to a series of strains of lactic streptococci used commercially as dairy starter cultures. with these transconjugants as donors the plasmid was exconjugated to two strains of lactobacillus helveticus and a commercially used strain of strep. thermophilus. there was evidence that the plasmid could transfer between isogenic derivatives of one of the strains of lact. helveticus. transfer from lact. helveticus ...19883146566
[difference between dental plaque formation by lactobacillus sp. and strep. mutans].human teeth were suspended in a basal medium with sucrose 10%; they were incudated for formation of dental plaques "in vitro". the microorganisms used were: strep. mutans, lactobacillus salivarius var. salivarius and a mixture of them. the following aspects were stablishad by scanning electron microscopy: 1) the teeth inoculated with strep. mutans had coccoid forms, lying on an organic film with spheric spaces in some sectors, and irreguar forms in others (fig. 1). 2) the teeth inoculated with l ...19761005789
effects of the ionophores monensin and tetronasin on simulated development of ruminal lactic acidosis in vitro.a continuous coculture of four ruminal bacteria, megasphaera elsdenii, selenomonas ruminantium, streptococcus bovis, and lactobacillus sp. strain lb17, was used to study the effects of the ionophores monensin and tetronasin on the changes in ruminal microbial ecology that occur during the onset of lactic acidosis. in control incubations, the system simulated the development of lactic acidosis in vivo, with an initial overgrowth of s. bovis when an excess of glucose was added to the fermentor. la ...19883223764
relationships of vaginal lactobacillus species, cervical chlamydia trachomatis, and bacterial vaginosis to preterm birth.the frequency of genital infection was compared among women in premature labor who delivered preterm (before 37 weeks), women in preterm labor who delivered at term, and control women who delivered at term. both groups of women in premature labor were younger and had more previous preterm births than did control women. women in premature labor who delivered preterm were more likely to experience rupture of membranes, intrapartum fever, and postpartum fever than were control women. the presence o ...19883336545
genetic transformation in lactobacillus sp. strain 100-33 of the capacity to colonize the nonsecreting gastric epithelium in mice.lactobacillus isolates able to colonize the surfaces of the nonsecreting epithelia in the stomachs of monoassociated ex-germfree mice were derived from lactobacillus acidophilus 100-33. strain 100-33 was originally isolated from pig feces and is unable to colonize the murine gastric epithelium. in experiments involving attempts genetically to transform the capacity to colonize the epithelium, cells of strain 100-33 were treated with muralytic enzymes and mixed with polyethylene glycol and genomi ...19883355132
identification of major contamination sources during processing of emulsion sausage.the extent of contamination of an emulsion type of sausage with lactic acid bacteria was determined along the processing line. this was done by aseptically removing sausages after five different processing stages (heat processing, chilling, cold storage, cutting down and packing). removed sausages were vacuum-packed and stored at 8 degrees c. the microbial growth was followed during storage and the microbiological shelf-life obtained at the different stages of the processing was determined. the ...19883275330
antimicrobial substance from a human lactobacillus strain.lactobacillus sp. strain gg, which was isolated from the feces of a normal person, produced a substance with potent inhibitory activity against a wide range of bacterial species. it inhibited anaerobic bacteria (clostridium spp., bacteroides spp., bifidobacterium spp.), members of the family enterobacteriaceae, pseudomonas spp. staphylococcus spp., and streptococcus spp., as demonstrated by a microbiological assay; however, it did not inhibit other lactobacilli. the inhibitory activity occurred ...19873307619
interstitial cystitis and the urethral syndrome: a possible answer.a study was made of 20 patients fulfilling the criteria customarily used for the diagnosis of interstitial cystitis. a possible infective aetiology was sought by culture of bladder tissue, catheter and midstream specimens of urine, and urethral swabs by methods capable of detecting fastidious bacteria as well as aerobic pathogens. all bladder biopsies showed the histological appearances usually associated with interstitial cystitis, and bacteria were isolated from the catheter specimens and/or b ...19892670041
plasmid-linked maltose utilization in lactobacillus ssp.five strains of lactobacillus plantarum and 4 strains of lactobacillus ssp. isolated from fresh meat contained between 1 and 5 plasmids ranging in mr from 1.3 to 49 mda. plasmid-curing studies suggested that maltose utilization is associated with a 49 mda plasmid (pml291) in lactobacillus sp. db29 and 34.5 mda plasmids in lactobacillus ssp. db27, db28 and db31. restriction digestion of pml291 and a putative plasmid deletion derivative, pml292, isolated from a maltose negative mutant of db29, gen ...19883139052
the recovery of lactobacillus sp. from the livers of healthy mice. 19734712300
lactobacillemia of amniotic fluid origin.four cases of amnionitis due to lactobacillus sp and three cases of bacteremia are reported. postpartum endometritis developed in all four cases. lactobacillus bacteremia occurred in two infants. a review of the literature on serious infections due to lactobacillus sp is also presented. the importance of considering the clinical role of any microorganism isolated in pure culture from symptomatic patients is discussed.19863725245
postspawning mortality of rainbow trout (salmo gairdneri) associated with lactobacillus.a lactobacillus sp. was consistently isolated from dead and moribund postspawning brood stock rainbow trout. pathology was characterized by massive chronic inflammation throughout the abdominal cavity and formation of pseudomembranes. the bacteria were most abundant in spent testes. repeated handling was considered to be the major stressor leading to this infection and subsequent high mortality.19854078969
the association between dietary sucrose consumption and microbial population shifts at six oral sites in man.sucrose-related microbial population shifts were evaluated at 6 oral sites in 22 volunteers, who consumed high-sucrose diets for 21 days followed by low-sucrose diets for 21 days. culturing was performed at 0, 12, 21, 33 and 42 days of the 6-week experiment. over 50,000 microbial isolates were characterized and analysed. analysis of initial cultures showed the following site-specific microbial characteristics of the 6 sites evaluated: (1) molar fissures harboured higher levels of neisseria speci ...19853861144
ontogeny of the bovine immune response.the ontogenesis of the bovine immune response was studied in three embryos (<40 days) and 106 fetuses of various ages. in the absence of overt antigenic stimulation, fetuses had lymphoid development of the thymus at 42 days of gestation, the spleen was structurally present at 55 days, and certain peripheral lymph nodes were present at 60 days. mesenteric lymph nodes were structurally present by 100 days of gestation, and lymphoid tissue of the gastrointestinal tract, particularly the lower ileum ...19734123777
[presence of streptococcus mutans and lactobacillus sp. in the stimulated saliva in a selected population]. 19863472803
oral health and factors related to oral health in polish schoolchildren.the oral health and factors related to oral health were surveyed in 168 schoolchildren, aged 10-12 years, attending one school in warsaw. the children were examined for dental caries and gingival status, dental plaque and salivary streptococcus mutans and lactobacillus counts. an interview on dietary habits and use of fluoridated toothpaste was also performed. the results showed a mean dmft of 4.7 in the sample. gingival index 1 and plaque index 3 were median scores for the majority of the child ...19873476246
effects of clostridium difficile, lactobacillus casei, bacillus subtilis and lactobacillus sp. on anomalous lower bowel function on germfree mice. 19816793297
[increase of caries in permanent teeth in relation to the numbers of streptococcus mutans and lactobacillus sp]. 19873483090
microbial flora of the lower genital tract of women in labour in zaria, nigeria.nine genera of microbes isolated from the lower genital tract of 187 women in labour in zaria have been identified. the work was undertaken to establish the nature of microorganisms in the lower genital tract of women in labour as a basis for further study. the isolates in order of prevalence were: candida albicans (20.9%), klebsiella sp (15.0%), escherichia coli (9.1%), streptococcus faecalis (6.4%), haemolytic streptococci (other than streptococcus pyogenes (2.7%), streptococcus viridans (2.1% ...19817007447
development of the normal gastrointestinal microflora of specific pathogen-free chickens.the development of the normal intestinal microflora of the small intestine, caecum and large intestine of specific pathogen-free (spf) chickens, was studied in the period from hatching to 84 days of age. no bacteria were detected in any of the sites at hatchery (day 1), but by day 3 significant levels of faecal streptococci and coliforms were isolated from all sites. the flora of the small intestine was limited to faecal streptococci and coliforms for the first 40 days and then lactobacilli beca ...19846229572
endogenous infection in mice with streptozotocin-induced diabetes. a feature of bacterial translocation.slc:ddy mice that received a single intraperitoneal injection of 200 mg/kg streptozotocin (stz) were examined for persistency of diabetes (changes of indigenous bacterial floras, and bacterial translocation. significant diabetes (increase in plasma glucose and decrease in insulin) was recognized 2 weeks after the injection, and persisted for 12 weeks. the numbers of aerobic gram-negative bacilli, staphylococci (including micrococci), and streptococci in caecal and oral floras were significantly ...19846240312
the microbial flora of smoked pork loin and frankfurter sausage stored in different gas atmospheres at 4 degrees c.the development of the microflora of smoked pork loin and frankfurter sausage was followed during storage in vacuum, n2 and co2 atmospheres at 4 degrees c. the total aerobic count on the smoked pork loin reached 10(7) organisms/g after 37 d in vacuum, 43 d in n2 and 49 d in co2. the corresponding value for the sausage was 77 d in vacuum, while the growth stopped at 6 x 10(40 organisms/g after 98 d in n2, and at 4 x 10(2) organisms/g after 48 d in co2. the predominant organisms on the fresh produ ...19836406420
ruminal metabolism of plant toxins with emphasis on indolic compounds.ruminal bacteria can perform biochemical transformations on plant constituents that may affect the health of ruminant animals. reactions carried out by ruminal bacteria on oxalates and some pyrrolizidine alkaloids include decarboxylation, hydrolysis and reduction steps. prior exposure of ruminal bacteria to these substances increases the rate of detoxification, indicating an adaptive response by the bacteria to these substrates. the formation of toxic substances by ruminal bacteria also occurs a ...19846373706
growth and end-product formation in fermenter cultures of brochothrix thermosphacta atcc 11509t and two psychrotrophic lactobacillus spp. in different gaseous atmospheres.the effects of different gaseous atmospheres were determined on the maximum specific growth rate (mumax) and end-product formation by brochothrix thermosphacta atcc 11509t, lactobacillus viridescens smricc 174 and lactobacillus sp. smricc 173 (homofermentative). the highest mumax-values for lact. viridescens (0.47/h) and broc. thermosphacta (0.49/h) were obtained in air. under anaerobic conditions mumax was reduced, an atmosphere containing co2 alone giving the greatest reduction. lactobacillus ...19846438042
characterization of lactobacillus sp. strain 100-37 from the murine gastrointestinal tract: ecology, plasmid content, and antagonistic activity toward clostridium ramosum h1.a gram-positive, nonsporulating, microaerophilic rod that had two colonial variants was obtained during a study in which anaerobic bacteria were isolated from murine gastrointestinal tracts and screened for cryptic plasmids. the rod (both colonial variants) was identified as a lactobacillus sp. (strain 100-37) by selective media, gas chromatography, and biochemical tests. in monoassociated, ex-germfree mice, the bacterium colonized the gastrointestinal tract and formed a thick, continuous layer ...19836651295
growth and end product formation of two psychrotrophic lactobacillus spp. and brochothrix thermosphacta atcc 11509t at different ph values and temperatures.lactobacillus viridescens, lactobacillus sp. strain 173 (homofermentative), and brochothrix thermosphacta atcc 11509t were studied at different ph values and temperatures in aerobic and anaerobic batch cultures. the growth rates were higher in aerobic than in anaerobic cultures. l. viridescens grew faster at ph 5.8 than at ph 6.3, whereas the opposite was true for b. thermosphacta. lactobacillus sp. strain 173 was inhibited in air or at 8 degrees c in anaerobic culture. b. thermosphacta did not ...19836660874
[spoilage microorganisms encountered in ultra-high temperature processed milk].40 strains of aerobic or facultative anaerobic microorganisms were isolated from a total of 37 spoiled ultra high temperature processed milk. 13 of them were identified as the genus bacillus. they were 6 b. cereus, 5 b. licheniformis, 1 b. brevis and 1 b. pumilus. the other 27 strains were nonsporeforming microorganisms, which included 5 yeasts, 2 pseudomonas sp., 3 streptococcus sp., 12 lactobacillus sp., 1 shigella sp., 1 aeromonas sp. and 3 micrococcus sp. results indicate that the spoilage o ...19846468029
comparison of bacterial and fungal adherence to vaginal exfoliated epithelial cells and human vaginal epithelial tissue culture cells.the adherence of four bacterial species and candida albicans to a new in vitro tissue culture model of human vaginal stratified squamous epithelium was investigated and compared with in vitro adherence to vaginal exfoliated cells. gardnerella vaginalis, group b streptococci, lactobacillus sp., and c. albicans adhered well to both exfoliated and tissue culture cells. similarly, a piliated fecal isolate of escherichia coli, but not a nonpiliated vaginal isolate of e. coli, adhered well to both cel ...19826120141
a prospective study of microorganisms in urine and bladder biopsies from interstitial cystitis patients and controls.interstitial cystitis (ic) is a chronic inflammatory condition of the bladder of unknown etiology. we tested the hypothesis that a microorganism would be found at higher prevalence in urine or bladder tissue from women with ic than from control women.19957855970
bacterial growth in ground beef prepared from electrically stimulated and nonstimulated muscles.ground beef samples prepared from electrically stimulated and nonstimulated biceps femoris and infraspinatus muscles were inoculated with lactobacillus sp., pseudomonas sp., acinetobacter sp., or a mixture of lactobacillus spp., pseudomonas spp., acinetobacter spp., moraxella sp., microbacterium thermosphactum, and erwinia herbicola. there were no significant differences in growth of various bacteria in ground beef made from electrically stimulated and nonstimulated muscles.19817195194
transtracheal aspiration in pulmonary infection in children with cystic fibrosis.six transtracheal aspirations (tta) and expectorated sputum specimens were collected from four children suffering from cystic fibrosis who had pulmonary infection. specimens obtained from both sites were cultured for aerobic bacteria and tta aspirates were also cultured for anaerobes. differences in bacteria isolated in tta and sputum aspirates were present in all instances. six isolates were recovered in both sites (three pseudomonas aeruginosa, two staphylococcus aureus and one aspergillus fla ...19836825749
[physiological activities of polysaccharide produced by lactobacillus sp]. 19827182529
sequence of ornithine decarboxylase from lactobacillus sp. strain 30a.a gene encoding biodegradative ornithine decarboxylase from lactobacillus sp. strain 30a was isolated from a genomic dna library and sequenced. primer extension analysis revealed two transcription initiation sites. the deduced amino acid sequence is compared with the amino acid sequences of five previously reported bacterial decarboxylases, and conserved pyridoxal phosphate motif residues are identified.19947961515
transit time of epithelial cells in the small intestines of germfree mice and ex-germfree mice associated with indigenous microorganisms.germfree mice housed in isolators under controlled environmental and nutritional conditions were associated with an intestinal microflora. these associated animals and germfree mice drawn from the same population were tested for the rate at which the epithelial cells transited from the crypts of lieberkuhn to the tips of the villi in their small intestines. the method for estimating the rate of transit of epithelial cells involved the use of liquid scintillation counting to determine the amount ...19817198427
fatty acid composition of lipids in the maternal diet and establishment of a lactobacillus sp. strain in the digestive tract of suckling gnotobiotic mice and gnotobiotic mice and rats, monoassociated with a homofermentative strain of lactobacillus sp. of intestinal origin, were fed either a commercial rodent chow a or a semisynthetic diet b. similar numbers of lactobacilli were established in their gastrointestinal tract whatever diet they ate. the lactobacilli were established in the digestive tract of the newborn of a mothers at 2 days but were hardly established in mouse or rat pups of a mothers than in their mouse homologues. comparative an ...19817270475
the normal cultivable microflora in upper jejunal fluid in healthy adults.bacteriological studies of uncontaminated upper jejunal fluid were performed in 85 normal subjects. fifty-three per cent of the samples were sterile (less than 10(1) cfu/ml). in 10% of the cases the total number of microorganisms exceeded 10(5) cfu/ml. the main groups of microorganisms isolated were streptococcus sp ('viridans group'), lactobacillus sp., veillonella parvula, actinomyces sp., haemophilus sp., corynebacterium sp., and candida albicans, each found in more than 10% of the subjects. ...19846372075
randomized comparison of ceftazidime versus clindamycin-tobramycin in the treatment of obstetrical and gynecological infections.a randomized comparison of ceftazidime versus clindamycin-tobramycin was performed for the treatment of obstetrical and gynecological infections. entry criteria were an oral temperature of greater than or equal to 38 degrees c and a clinical diagnosis of endometritis, salpingitis, or pelvic cellulitis after hysterectomy. all patients with endometritis had cultures of intrauterine material obtained via a transcervical single-lumen catheter. the patients with pelvic cellulitis had material from th ...19836360038
influence of salivary levels of mutans streptococci on colonization of crown margins: a longitudinal study.initial colonization of mutans streptococci in 36 crown margins of 21 patients was studied. before cementation, salivary concentrations of mutans streptococci and lactobacillus sp, microorganisms associated with dental caries, were determined. within 1 week 25% of the selected crown margins in subjects with low salivary levels of mutans streptococci and 89% in subjects with high salivary levels were colonized. high salivary counts of lactobacilli seemed to reflect conditions that enhanced the ri ...19938483132
[cloning and expression of a plasmid pbs195 gene, determining oxygenase activity, in escherichia coli cells].plasmid pbs195, detected in a strain of lactobacillus sp. isolated from long-living persons, has a broad host range, including gram-positive and gram-negative microorganisms [1]. plasmid-harboring colonies of the strain escherichia coli hb101 give a color reaction with catechol. this indicates that genes mediating the activity of oxygenase are present in this plasmid. the high activity level of this enzyme, mediated by pbs195, and substrate specificity, which has not bee detected in any known me ...19957721057
identification of a bacillus thuringiensis gene that positively regulates transcription of the phosphatidylinositol-specific phospholipase c gene at the onset of the stationary phase.a transcriptional analysis of the phosphatidylinositol-specific phospholipase c (plca) gene of bacillus thuringiensis indicated that its transcription was activated at the onset of the stationary phase in b. thuringiensis but was not activated in b. subtilis. the b. thuringiensis gene encoding a transcriptional activator required for plca expression was cloned by using a b. subtilis strain carrying a chromosomal plca'-'lacz fusion as a heterologous host for selection. this trans activator (desig ...19968631661
aggregation-promoting factor in human vaginal lactobacillus strains.a total of 60 lactobacillus sp. strains were examined for expression of auto-aggregation and cell surface hydrophobicity. isolates were obtained from the vagina of healthy women (n = 20). the results obtained showed that the occurrence of cell surface hydrophobicity correlated with auto-aggregative activity in 12 homofermentative lactobacillus sp. strains. the aggregation mechanism was mediated by the presence of an aggregation promoting factor (apf) in one lactobacillus sp. strain, hv 142. apf ...19979395055
survival and growth of selected pathogens in fermented kocho (ensete ventricosum).a study was conducted to investigate the antagonistic potential of fermented kocho, aqueous extract of fermented kocho and spent media from lactic acid bacteria (lab) isolated from it on salmonella sp, pseudomonas aeruginosa, klebsiella sp, bacillus cereus and staphylococcus aureus. fermented kocho (ph 4.3) inhibited growth of the test bacteria soon after their introduction into the food. the spent media from all of the four lab, isolated from fermented kocho, namely pediococcus sp, leuconostoc ...19947867544
low surface tension calcium hydroxide solution is an effective antiseptic.the antimicrobial effects of a saturated calcium hydroxide solution, and in combination with 10% and 20% detergent, were evaluated on streptococcus faecalis, streptococcus sanguis, streptococcus mutans, streptococcus salivarius, neisseria sp., diphtheroid, staphylococcus aureus, lactobacillus sp., staphylococcus epidermidis, bacillus subtilis and candida albicans. the saturated calcium hydroxide solution was effective against only four of the 11 microorganisms studied over a 60-min exposure time ...19947806414
quality assessment of glycopeptide susceptibility tests: a european collaborative study. european glycopeptide resistance group.the ability of seventy clinical laboratories in nine european countries to detect glycopeptide resistance in gram-positive bacteria was investigated. results of routine tests were compared with those on the same strains by a reference method in national co-ordinating laboratories. in addition, control strains were tested by some of the participants. errors in reporting susceptibility of staphylococcus aureus to teicoplanin and vancomycin and coagulase-negative staphylococci to vancomycin were < ...19979552711
[candida parapsilosis in reproductive organ infections].frequency of occurrence of strains of c. parapsilosis, their susceptibility to drugs and importance in pathogenesis of vaginal mycosis, was evaluated. strains of c. parapsilosis were isolated in 44 cases which comprises 1.34% of a total number of 3275 tested candida-like strains. vaginal mycoses occurred in presence of lactobacillus sp., normal ph values of vaginal contents, whereas leukocytosis was differing. following drugs were used for testing susceptibility of c. parapsilosis: nystatin, pim ...19938309308
attachment of streptococcus faecium, to the duodenal epithelium of the chicken and its importance in colonization of the small intestine.the counts of streptococcus faecium sy1 in the duodenums of gnotobiotic chicks exceeded the counts in their crops, indicating that multiplication was occurring in the anterior small intestine. this growth was related to adhesion to the gut wall which could be demonstrated by viable counts of macerated washed duodenal tissue. scanning electron microscopy demonstrated that adhesion occurred in restricted areas on the surface of the villus, and transmission studies showed the presence of a thick ex ...19817247397
[characteristics of lactobacillus species strains isolated from reproductive organs in various clinical cases].the study was aimed at detection of presence of lactobacillus species bacteria in 167 samples of material derived from vagina of patients with cervix uteri (22 samples), chronic vaginosis (100 samples) and from women after treatment of inflammatory diseases (45 samples). out of 100 isolated strains of lactobacillus only 13 belonged to l. acidophilus. they were mainly present (10 strains) in patients after effective treatment of inflammatory diseases of reproductive organs. remaining strains belo ...19938309296
behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various this study, behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various lactobacillus sp. were determined. ph values of the skim milk samples were also examined during fermentation. the amount of produced lactic acid and diacetyl/acetoin productions of the lactobacillus sp. were estimated. antimicrobial effects of the lactobacilli on y. enterocolitica and a. hydrophila were also determined by an agar diffusion method. while y. enterocolitica was n ...19968638435
effect of antioxidative lactic acid bacteria on rats fed a diet deficient in vitamin e.lactic acid bacteria, including bifidobacterium, with antioxidative activity were selected by in vitro screening. the effect of the antioxidative activity was investigated by in vivo experiments using rats that were deficient in vitamin e. in the first stage of screening, 570 strains were examined; intracellular cell-free extracts of 19 strains (16 lactobacilli, 2 streptococci, and 1 lactococci) had antioxidative activity as determined by an assay using rat liver microsomes and thiobarbituric ac ...19938227652
purification and properties of ornithine decarboxylase from lactobacillus sp. 30a.inducible ornithine decarboxylase from lactobacillus sp. 30a has been purified to homogeneity as judged by ultracentrifugation and gel electrophoresis. unlike histidine decarboxylase from the same species (a pyruvoyl enzyme), ornithine decarboxylase is a pyridoxal phosphate enzyme. the purified enzyme is specific for l-pornithine (km 1.7 mm; specific activity, 150 to 200 mumol min-1 mg-1 at 37 degrees c) and is inhibited by various homologous omega-amino acids, amines, and polyamines. the native ...19807380847
the effect of vaginal microbes on in vivo and in vitro expression of human papillomavirus 16 e6-e7 genes.since other viruses can influence expression of human papillomavirus (hpv) 16 e6-e7 genes in vitro, this study addressed whether specific vaginal bacteria do so as well. the vaginal microflora of 18 women with cervical intraepithelial neoplasia (cin) or normal histology and hpv 16 infection, was evaluated by quantitative culture. expression of hpv 16 e6-e7 oncogenes was assessed in exfoliated cervical cells by quantitative polymerase chain reaction. hpv 16 expression was also quantitated in cask ...19999892986
[intravaginal bacterial flora and clinical significance of granulocyte elastase and ph determination].in 95 patients with abnormal vaginal and cervical secreta (49 pregnant women and 46 non-pregnant women), the relation between intravaginal flora and intravaginal granulocyte elastase (elastase) and ph was investigated. the results were as follows. 1) gram-positive bacteria were detected in the vaginal secreta at a high rate (87/144, 60.4%), and it mainly consisted of lactobacillus sp. (67/142, 46.5%). it was followed anaerobia (26/144, 18.1%) and fungi (26/144, 18.1%). 2) the patients with cervi ...19979212368
[the probiotic correction of microecological and immune disorders in gastroduodenal pathology in children].the state of microbiocenosis was studied and the level of immunoglobulins was determined in the gastric juice and feces of children with chronic diseases of the digestive system. in 20% of patients an increase in the contamination of the gastric biotope with opportunistic microflora was established. the isolation rate of helicobacter pylori was 56%. the detection of h. pylori was found to be accompanied by the aggravation of the form and course of gastritis. in cases of helicobacter-associated p ...19968701669
dental disease, caries related microflora and salivary iga of children with severe congenital cardiac disease: an epidemiological and oral microbial survey.the objectives were to determine levels of dental caries, plaque accumulation, gingival inflammation, knowledge of dental health practices, and oral bacterial loading of s. mutans, lactobacillus sp., candida sp., and salivary iga in the mouths of children afflicted with severe congenital heart disease. a total of 60 children from the cardiac units of the hospital for sick children and guys hospital paediatric department were compared with 60 case-matched control children from the department of o ...19968784915
spray-drying of bacteriocin-producing lactic acid bacteria.cell survival, cellular damage, and antagonistic activity were investigated after spray-drying of four bacteriocin-producing strains of lactic acid bacteria: lactococcus lactis subsp. lactis 140, isolated from natural whey culture and producing a narrow-inhibitory spectrum bacteriocin); l. lactis subsp. lactis g35, isolated from pizza dough and producing nisin; lactobacillus curvatus 32y and lactobacillus sp. 8z, isolated from dry sausages. trials were performed with bacteria suspended in skimme ...199910419271
exchange of glutamate and gamma-aminobutyrate in a lactobacillus strain.lactobacillus sp. strain e1 catalyzed the decarboxylation of glutamate (glu), resulting in a nearly stoichiometric release of the products gamma-aminobutyrate (gaba) and co2. this decarboxylation was associated with the net synthesis of atp. atp synthesis was inhibited almost completely by nigericin and about 70% by n,n'-dicyclohexylcarbodiimide (dccd), without inhibition of the decarboxylation. these findings are consistent with the possibility that a proton motive force arises from the cytopla ...19979150237
identification of lactic acid bacteria constituting the predominating microflora in an acid-fermented condiment (tempoyak) popular in malaysia.tempoyak is a traditional malaysian fermented condiment made from the pulp of the durian fruit (durio zibethinus). salt is sometime added to proceed fermentation at ambient temperature. in various samples obtained from night markets, lactic acid bacteria (lab) were the predominant microorganisms, ranging from log 8.4 to log 9.2 cfu g(-1). no other microorganisms were present to such a level. these samples contained reduced amount of saccharose, glucose and fructose but increased amount of d- and ...200111205946
antimicrobial activity of food-related penicillium sp. against pathogenic bacteria in laboratory media and a cheese model penicillium species (n = 34) and geotrichum candidum (n = 11) grown on czapek dox and brie agar were tested for their ability to suppress growth of pathogenic bacteria. ten out of 13 p. camemberti showed antagonistic activity while the other species did not interact significantly with the bacterial growth. the order of inhibition was: gram-negative bacteria and bacillus cereus > listeria monocytogenes, lactococcus sp. > micrococcus sp. whereas lactobacillus sp., staphylococcus aureu ...19979246777
ecofunctional enzymes of microbial communities in ground water.biolog technology was initially developed as a rapid, broad spectrum method for the biochemical identification of clinical microorganisms. demand and creative application of this technology has resulted in the development of biolog plates for gram-negative and gram-positive bacteria, for yeast and lactobacillus sp. microbial ecologists have extended the use of these plates from the identification of pure culture isolates to a tool for quantifying the metabolic patterns of mixed cultures, consort ...19979299711
properties of porcine and yogurt lactobacilli in relation to lactose intolerance.lactobacilli that had been isolated from the stomach of piglets were tested for properties relevant to the production of fermented milk products for consumption by lactose-intolerant humans. the strains were characterized for beta-galactosidase activity, the ability to reduce the lactose concentration of milk, viability, and ph of the fermented milk over a 30-d period. strains that had favorable attributes were studied further, and the optimal ph for beta-galactosidase activity, ability to grow ...19979361203
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