l-glutamate-glyoxylate aminotransferase in lactobacillus plantarum.glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha-oxoglutaric acid has been isolated and purified 84-fold from extracts of lactobacillus plantarum. purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. the molecular weight of the enzyme estimated by sepharose 4b gel filtration amounts to 37.000. micaelis constants for glyoxylate and glutamate are corresponding to 6.25 x 10( ...19755859
lactobacillus plantarum undecaprenyl pyrophosphate synthetase: purification and reaction requirements. 19768010
allosteric and non-allosteric phosphofructokinases from lactobacilli. purification and properties of phosphofructokinases from l. plantarum and l. acidophilus.phosphofructokinase (atp : d-fructose-6-phosphate 1 phosphotransferase, ec from two different lactobacilli, lactobacillus plantarum and lactobacillus acidophilus were isolated and purified. both enzymes have a molecular weight of 154 000 and consist of four subunits of identical size. antisera from sheep immunized against the purified phosphofructokinase from l. plantarum showed immunologic cross reaction with the enzyme from l. acidophilus. in spite of the close molecular relationship ...197715608
ph metric method for the determination of nicotinic acid in plasma.the acidimetric method for the determination of nicotinic acid (na) using lactobacillus plantarum atcc 8014 (lactobacillus arabinosus 17-5) has been simplified and thus made less time consuming, and the sensitivity has been increased fivefold by replacement of the titration by a ph determination. as the regression of the decrease in ph on the amount of na was found linear within a range of 1 to 4 ng of na per ml, the calculations were performed according to the slope-ratio principle. the na conc ...197716033
transport of pantothenic acid in lactobacillus plantarum. 197718275
binding of lectins to streptococcus mutans cells and type-specific polysaccharides, and effect on adherence.the lectin concanavalin a (con a) agglutinated the cells of 13 of 15 strains of the seven serotypes of streptococcus mutans in an 18-h incubation period. strains of types a, d, f, and g agglutinated within 2 h. strains of a, d, and f were also agglutinated in 2 h by the castor bean lectin rca. s. sanguis, s. salivarius, s. bovis, actinomyces viscosus, a. naeslundii, and lactobacillus plantarum were agglutinated within 2 h. the s. mutans type f polysaccharide was precipitated by con a. the a, b, ...197722491
a radiometric microbiologic assay for the biologically active forms at niacin.a radiometric microbiologic assay has been developed for the determination of niacin in biologic fluids. lactobacillus plantarum produced 14co2 from l-[u-14c] hr malic acid in quantities proportional to the amount of niacin present. the assay is specific for the biologically active forms of niacin in humans. thirty normal hemolysates were analyzed and the values ranged from 13.0 to 17.8 micrograms niacin/ml rbc (mean = 15.27 +/- 1.33 s.d.). good recovery and reproducibility studies were obtained ...1979120418
reaction mechanism of non-allosteric phosphofructokinase.the reaction mechanism of the non-allosteric phosphofructokinase from lactobacillus plantarum was investigated by initial-rate bisubstrate kinetics and product inhibition kinetics adn by the measurement of equilibrium isotope exchange in the presence of various substrate and product concentrations. the reaction mechanism is clearly sequential. the product inhibition and equilibrium isotope-exchange patterns are consistent with an ordered bi-bi reaction sequence with fructose 6-phosphate as the l ...1978149659
susceptibility to adjuvant-induced arthritis among germfree, specific-pathogen-free, and conventional rats.germfree f344 rats developed severe arthritis with 100% incidence after a single intradermal inection of either squalane containing 0.5 mg of heat-killed mycobacterium bovis bcg or a water-in-oil emulsion containing 0.2 mg of peptidoglycan derived from staphylococcus epidermidis. conventional f344 rats developed less-severe arthritis with 20% incidence for heat-killed bcg and 0% incidence for peptidoglycan. specific-pathogen-free rats showed an intermediate susceptibility between germfree and co ...1979160888
characterization of undecaprenol kinase from lactobacillus plantarum.a membrane-bound undecaprenol kinase from lactobacillus has been identified by observing the atp-dependent phosphorylation of [14c]undercaprenol. the product of this reaction was shown to be [14c]undecaprenyl monophosphate by comparison of its chromatographic mobilities with authentic undecaprenyl monophosphate. it was shown that 32p from [gamma-32p]atp was incorporated into undecaprenyl monophosphate. the kinase was partially solubilized by a variety of methods utilizing triton x-100. both the ...1979224936
analysis of bacterial biotin-proteins.the biotin-protein populations in several bacterial strains were analyzed by solubilization of [3h]biotin-labeled cells with sodium dodecylsulfate followed by electrophoresis on polyacrylamide gels containing the detergent. a variety of patterns of biotin-labeled polypeptide chains was seen, ranging from a single biotin-protein in escherichia coli, corresponding to the biotin carboxyl carrier protein component of acetyl-coa carboxylase, to multiple species in enterobacter aerogenes, pseudomonas ...1975235315
properties of a membrane-bound cardiolipin synthetase from lactobacillus plantarum.cardiolipin (cl) synthetase of lactobacillus plantarum 17-5 catalyzed the stoichiometric conversion of 2 mol of phosphatidylglycerol to 1 mol of cl. the enzyme activity was linear with time for 30 min at 37 c and with protein concentration between 20 and 200 mug of protein per ml. the enzyme was membrane associated, had a ph optimum of 5.1 in phosphate buffer, and was not stimulated by mg2+, and the activity was not affected by the addition of ethylenediaminetetraacetic acid, cytidine diphosphat ...1975239926
the effects of additional flora on the response of salmonella mutants lodged in the gastrointestinal tract.a histidine auxotroph of salmonella typhimurium, strain ta1538, will lodge for several months in the gastrointestinal tract of otherwise germ-free rats and of rats additionally associated with bacteria characteristic of the normal flora such as lactobacillus plantarum and bacteroides vulgatus. in the presence of the additional flora, the concentration of strain ta1538 is diminished in the stomach but not in the lower gastrointestinal tract or in the feces. following the ingestion of 2-nitrofluor ...1977318921
properties of the ames salmonella mutants lodged in the gastrointestinal tract of gnotobiotic association of the histidine auxotroph of salmonella typhimurium (strain ta1538) within the gastrointestinal tract of otherwise germ-free sprague-dawley rats is maintained during observations for up to 7 months. the bacteria exceed concentrations of 10(7)/g in the forestomach and exceed concentrations of 10(8)/g in the lower bowel and feces. when carcinogens are ingested, the number of revertants in the feces increases. the ingestion of structurally related compounds which are not mutagenic t ...1977335870
[role of normal microflora in the treatment of vibrio carrier state in rat gnotobionts].materials on the study of interactions between vibrio cholera el tor and some indigenous bacteria of human intestinal tract, which proved to be active antagonists in vitro, are presented in this work. experiments were conducted on gnotobiotic rats (fisher), using various combinations and variants of the order of the microorganism administration; there were revealed no antagonistic relations between virbrio cholerae el tor and escherichia coli m-17, lactobacillus fermenti, lactobacillus plantarum ...1978352437
adjuvanticity of lactobacilli. i. differential effects of viable and killed bacteria.the adjuvanticity of lactobacillus brevis and lactobacillus plantarum was the subject of this study. the latter was the better adjuvant in both delayed hypersensivity and antibody formation to sheep red blood cells. viable l. plantarum stimulated exclusively the delayed hypersensivity, where heat-killed bacteria had an adjuvant effect on antibody formation. for optimal adjuvant effects lactobacilli had to be injected in a dose of 10(8) into the same site as the antigen. viable lactobacilli and t ...1979387312
in vitro antimicrobial activity of some cyclic hydroxamic acids and related lactams.against enterobacter aerogenes 13048, serratia marcescens 13880, klebsiella pneumoniae 10031, pseudomonas aeruginosa 10145, escherichia coli 9723, lactobacillus casei 7469, lactobacillus plantarum 8014, leuconostoc dextranicum 8086, and streptococcus faecalis 8043, the mean minimal inhibitory concentrations of three cyclic hydroxamic acids, 3-amino-3,4-dihydro-1-hydroxycarbostyril, the 6-chloro analog, and the 7-chloro analog, were 0.6, 0.6, and 0.2 micrograms/ml, and those of the corresponding ...1978400828
[behavior of some microorganisms cultivated in the presence of irradiated maize starch].the bactericidal effect of water soluble radiolysis products of maize starch on escherichia coli cultures declines when the initial population grows. this toxicity varies with the culture environment and the bacterial species: bacillus subtilis, lactobacillus plantarum, streptococcus faecalis, enterobacter sp. moreover, for the eucaryote cell saccharomyces cerevisiae, wild strain or muting "small colonies", only the generating time is altered by the active parts of radiolysis products. the resul ...1977410543
comparative studies of lactate dehydrogenases in lactic acid bacteria. amino-acid composition of an active-site region and chemical properties of the l-lactate dehydrogenase of lactobacillus casei, lactobacillus curvatus, lactobacillus plantarum, and lactobacillus acidophilus.the molecular weight, the amino acid composition and the n-terminal and c-terminal amino acids of two allosteric (lactobacillus casei, l. curvatus) and two non-allosteric (l. plantarum, l. acidophilus) l-lactate dehydrogenases, purified to homogeneity by affinity chromatography, were determined. the amino acid composition of the only tryptic peptide unequivocally common to the fingerprints of the 4 enzymes is virtually identical with that of the arginine peptide, called arg6 of the the substrate ...1977411654
on the relationship between glycerophosphoglycolipids and lipoteichoic acids in gram-positive bacteria. ii. structures of glycerophosphoglycolipids.1. eight glycerophosphoglycolipids were isolated from six gram-positive bacteria. besides sn-glycero-1-phospho-beta-gentiobiosyldiacylglycerol (i) and sn-glycero-1-phospho-alpha-kojibiosyldiacylglycerol (ii), three novel structures have been established: 1,2-di-o-acyl-3-o-[6-(sn-glycero-1-phospho)-alpha-d-glucopyranosyl-(1 leads to 2)-(6-o-acyl-alpha-d-glucopyranosyl)]glycerol (iii), 1,2-di-o-acyl-3-o-[6-(sn-glycero-1-phospho)-beta-d-glucopyranosyl-(1 leads to 6)-alpha-d-galactopyranosyl-(1 lead ...1978416848
development of listeria monocytogenes in monoxenic and polyxenic beef minces.the objective of this study was to determine the multiplication possibilities of listeria monocytogenes in beef minces with a defined microflora (gnotoxenic). selective counts were made for each species and each bacterial association for a period of 17 days after inoculation of the axenic meat. the concentration of l. monocytogenes did not increase, but remained constant in monoxenic meats. associated with lactobacillus plantarum, the number of listeria tended to decrease. conversely, in associa ...1978417516
identification of antimicrobial alpha-hydroxyacids in lactobacillus plantarum-fermented animal protein.during the fermentation of animal protein by lactobacillus plantarum, highly volatile and less volatile antimicrobial substances are formed. the antimicrobial effect of the latter fraction depends on the content of alpha-hydroxyacids in the fermentation product. these acids were isolated from the fermentation mixture and identified by chemical methods. the main components are racemic forms of lactic, alpha-hydroxyisovaleric and alpha-hydroxyisocaproic acids.1979424089
serological studies on chemostat-grown cultures of lactobacillus fermentum and lactobacillus plantarum.lactobacillus fermentum nctc 6991 and lactobacillus plantarum ncib 7220 were grown in a chemostat in the diffusible fraction of complex medium at ph 6.0 with glucose limitation. organisms grown at different dilution rates (d) were injected into rabbits, and the resultant antisera were examined for reactivity with antigens previously isolated from batch-grown organisms. for l. fermentum, antisera obtained on injecting cells grown at d = 0.5 h-1 contained a significantly higher level of antibody r ...1979457268
inhibition of macrophage migration by muramyl the capillary tube migration system a synthetic muramyl dipeptide (mdp; n-acetylmuramyl-l-alanyl-d-isoglutamine), a part of bacterial cell wall peptidoglycans, inhibited the migration of peritoneal exudate macrophages from normal guinea pigs or rats. the migration inhibition was also caused by some mdp-containing peptidoglycan fragments from cell walls of lactobacillus plantarum and staphylococcus epidermidis. the migration inhibition could not be explained on the basis of macrophage migratio ...1979457276
lysozyme sensitivity of pantothenate-deficient lactobacillus plantarum grown with exogenous fatty acids.a pantothenic acid deficiency in lactobacillus plantarum reduces lipid synthesis, prevents normal uptake and retention of extracellular amino acids and markedly increases sensitivity of these cells to lysozyme induced lysis. pantothenate-deficient cells provided with exogenous fatty acids synthesize additional lipids and express nearly normal solute transport activities. the present study has shown that such cells retain a heightened sensitivity to lysozyme induced lysis. these observations indi ...1979476132
electron diffraction studies of the peptidoglycan of bacterial cell walls.peptidoglycan of the gram-positive bacterium lactobacillus plantarum was adsorbed onto hydrophilic crystalline films of graphitic oxide. destruction by radiation damage was reduced by using a device which permitted scanning of the specimen for several hours through a focussed electron beam of low current density (2.5 x 10(-5) a/cm2). in additition to the sharp debye-scherrer rings caused by graphitic oxide, peptidoglycan causes a diffuse debye-scherrer ring in the region of 4.5 a. this can be in ...1979494435
synergistic effect of polyriboinosinic acid:polyribocytidylic acid and either bacterial peptidoglycans or synthetic n-acetylmuramyl peptides on production of adjuvant-induced arthritis in rats.lewis rats developed polyarthritis after a single injection of a water-in-oil emulsion containing various peptidoglycans (pgs) derived from lactobacillus plantarum. a copolymer of polyriboinosinic acid and polyribocytidylic acid markedly potentiated the arthritogenicity of these pgs. the synthetic adjuvants n-acetylmuramyl-l-alanyl-d-isoglutamine (murnac-l-ala-d-isogln) and murnac-l-ala-d-gln were non-arthritogenic, but they did produce severe arthritis when mixed in a water-in-oil emulsion with ...1979546792
[influence of some thiamine antagonists on the growth of kloeckera apiculata, lactobacillus fermenti and lactobacillus arabinosus (author's transl)]. 1977557103
[chemical properties and immunological activities of peptidoglycans isolated from l-3 enzyme lysate of lactobacillus plantarum cell wall (author's transl)]. 1978658883
dihydrofolate reductases in some folate-requiring bacteria with low trimethoprim susceptibility.dihydrofolate reductases from the folate-requiring strains streptococcus faecalis atcc 8043, lactobacillus casei atcc 7496, and pediococcus cerevisiae atcc 8081, as well as from lactobacillus arabinosus, which is not dependent on exogenous folate, were isolated, and their properties were compared to reductases of escherichia coli b, staphylococcus aureus, and rat liver reductase. an inhibition profile with six different inhibitors revealed significant differences among all enzymes. all lactobaci ...1978686702
substrate specificity of undecaprenyl pyrophosphate synthetase from lactobacillus plantarum. 1978728420
radioimmunoassay for pantothenic acid in blood and other tissues.we describe a radioimmunoassay for pantothenic acid in biological tissues. d-pantothenic acid was conjugated with bovine serum albumin by use of a bromoacetyl derivative of pantothenic acid, and antibody to this antigen was raised by injecting it into the foot pads of rabbits. for the radioimmunoassay, a 100-fold dilution of the resulting antiserum was incubated with radiolabeled pantothentic acid. the antibodies were precipitated and dissolved, and the radioactivity of the solution was measured ...1979761345
synthesis and microbiological activities of beta-(1-chloro-2-naphthyl) alanine and beta-(1-bromo-2-naphthyl) alanine.beta-(1-chloro-2-naphthyl)alanine and beta-(1-bromo-2-naphthyl) alanine were synthesized by ammonolysis of the corresponding alpha, 1-dihalo-2-naphthalenepropanoic acids derived from 1-nitro-2-naphthylamine by diazotization and condensation with acrylic acid in the presence of cuprous halides. the two analogs as well as the previously reported beta-(2-naphthyl)alanine and beta-(1-naphthyl)alanine were studied as growth inhibitors of escherichia coli 9723, leuconostoc dextranicum 8086, and lactob ...1976768475
dynamics of mixed cultures of lactobacillus plantarum and propionibacterium shermanii.the mixed culture of lactobacillus plantarum and propionibacterium shermanii grown anaerobically in glucose minimal medium exhibits features typical of a commensal interaction even though a number of complicating factors, such as a large maintenance requirement of l. plantarum and inhibition of growth of p. shermanii at low ph, are present. a simple mathematical model of the system is presented and is shown to reproduce rather well some of the features of the continuous mixed culture system in b ...1976773448
acyl carrier protein metabolism and regulation of fatty acid biosynthesis by lactobacillus plantarum.endogenous fatty acid biosynthesis in the bacterium lactobacillus plantarum is greatly decreased upon addition of exogenous fatty acids (henderson, t.a., and mcneil, j.j. (1966) biochem. biophys. res. commun. 25, 662-669). we have demonstrated the presence of five pantothenate-containing compounds in l. plantarum which have been identified by co-chromatography with authentic samples: pantothenate, 4'-phosphopantetheine, 3'-dephosphocoenzyme a, coenzyme a, and acyl carrier protein (acp). the conc ...1976780354
synthesis and microbiological activities of some monohalogenated analogs of tyrosine.2-chlorotyrosine and 2-bromotyrosine, as well as the previously reported 2-fluorotyrosine, were synthesized by hydrolysis of the condensation products from the appropriate benzyl bromide and ethyl acetamidomalonate and were compared with the corresponding 3-halotyrosines as growth inhibitors of escherichia coli 9723, streptococcus faecalis 8043 and lactobacillus plantarum 8014. in contrast to the 2- and 3-fluorotyrosines which were equally effective as growth inhibitors, the 2-chloro- and 2-brom ...1975803244
determination of the isotope distribution in malate-14c with fumarase-negative lactic acid fumarase activity could be found in whole cells or in cell-free crude extracts from leuconostoc mesenteroides or lactobacillus curvatus. the degradation of l-malate-4-14c by these organisms yielded more than 95% of the label as 14co2. it is therefore recommended that these organisms, rather than lactobacillus plantarum, should be used in the determination of isotope distribution in l-malate-14c, since l. plantarum exhibits a significant fumarase activity and thus randomizes malate prior to th ...1977843172
thymidine content in commercially prepared media.the thymidine content of a number of media now available commercially was determined, using lactobacillus arabinosus atcc 8014. the thymidine concentration in most liquid media was so high (greater than 0.01 microng/ml) that, without further treatment, these media could not be used for the testing of sulphonamides, trimethoprim or combinations thereof, more especially if bactericidal action was being investigated. low and tolerable thymidine amounts were found in some agar media. considerable va ...1977848219
lipid activation of undecaprenyl pyrophosphate synthetase from lactobacillus plantarum. 1977880287
a kinetic analysis of the processive enzyme lactobacillus plantarum exoribonuclease.oligonucleotide chains consisting of adenosine residues and ranging from 1 to 70 residues in length have been tested as substrates or inhibitors with lactobacillus plantarum exoribonuclease (ec3.1.4.20). the kinetic constants v, km, and ki are all chain-length dependent. ki decreases with increasing chain length to a minimum for oligonucleotides seven residues in length and then begins to increase slightly. kinetic plots indicate that the oligonucleotides are almost all competitive inhibitors of ...1977890565
isolation, extraction, and measurement of acetylcholine from lactobacillus plantarum.the isolation, extraction, and spectrophotometric determination of acetylcholine from lactobacillus plantarum atcc 10241 is described. acetylcholine was extracted with a mixture of sodium tetraphenylboron-butylethylketone-acetonitrile and was measured enzymatically at 340 nm.1977907345
[lactobacilli of freshwater fishes].normal microflora in the intestinal tract of fishes inhabiting fresh-water reservoirs includes lactic bacteria. the number of the bacteria depends on the animal species, the composition of food, the age, and the season. the highest number of these microorganisms (hundreds of millions per gram of the intestinal content) is found in carps. enterococci are most often encountered in fishes inhabiting ponds: streptococcus faecalis andrewes a. horder, str. faecium orla-jensen, str. bovis orla-jensen. ...1977909475
damped oscillations in continuous culture of lactobacillus plantarum.lactobacillus plantarum exhibited long-period damped oscillations when grown aerobically with glucose as the rate-limiting substrate. hydrogen peroxide accumulated in the cultures and its concentration also exhibited damped oscillations. it is suggested that inhibition of growth by hydrogen peroxide is involved in the production of the damped oscillations of microbiol population density.1976932674
microbiological assay with lactobacillus plantarum for detection of adulteration in orange juice.a microbiological assay has been developed to help detect adulteration in orange juice. under standard assay conditions with diluted orange juice, the growth of lactobacillus plantarum is proportional to the concentration of juice in the assay mixture. imitation orange beverages did not support growth. growth was also independent of the normal levels of common beverage ingredients such as sugar, acids, butylated hyroxyanisole, and orange oil. commercial orange juices reconstituted from concentra ...1976993188
isolation and purification of d-alanyl-meso-2, 6-diaminopimelic acid endopeptidase of streptomyces l-3 enzyme using soluble substrates of known chemical structure from lactobacillus plantarum cell wall digests.d-alanyl-meso-2, 6-diaminopimelic acid (d-alanyl-meso-a2pm) endopeptidase was isolated and purified from a crude streptomyces l-3 enzyme preparation by ion exchange chromatography and isoelectric focusing in a density gradient. during its purification, its hydrolytic activity was assayed on cell walls of lactobacillus plantarum atcc 8014 and soluble glycopeptides and peptides, of known chemical structures, prepared enzymatically from these cell walls. a fraction with an isoelectric point of ph 7 ...19761016217
structural requirements for arthritogenicity of peptidoglycans from staphylococcus aureus and lactobacillus plant arum and analogous synthetic compounds.the comparative studies on the arthritogenicity of chemically well defined peptidoglycans (pg)2 from the cell walls of staphylococcus aureus (fda 209p) and lactobacillus plantarum (atcc 8014) showed that 1) a polymer of disaccharide-peptide with or without the presence of n-acetylglucosaminyl-ribitol-teichoic acid produced severe arthritis; 2) oligosaccharide-peptides with or without the special structure (n-acetylglucosaminyl-ribitol-teichoic acid in s. aureus, a polymer of rhamnose and glucose ...19761083878
the relationship between nitro group reduction and the intestinal microflora.the capacity of rats to reduce a 25-mg dose of p-nitrobenzoic acid (pnba) was measured by quantifying the amount of this compound recovered in the urine as p-aminobenzoic acid (paba) and its conjugates. it was found that germfree rats converted approximately 1% of pnba to paba; in conventional rats the conversion was approximately 25%. various bacteria isolated from the rat cecum were selectively associated with germfree rats and it was demonstrated that these bacteria colonized their gastrointe ...19751097637
synthesis and antibacterial activities of some chloro analogs of 3 amino-3,4-dihydro-1-hydroxycarbostyril.the effects of a chloro substituent upon the microbiological activities of 3-amino-3,4-dihydro-1-hydroxycarbostyril were determined. the 5-, 6-, and 7-chloro analogs were synthesized by reductive cyclizations of the appropriately chloro-substituted o-nitrophenylalanines, while the 8-chloro analog was obtained from the n-trifluoroacetyl-3-chloro-2-nitrophenylalanine ethyl ester. all of these compounds were observed to inhibit the growth of escherichia coli 9723, leuconostoc dextranicum 8086, and ...19751097694
heterogeneous elevation of amino acid transport rates in pantothenate-and lipid-deficient lactobacillus plantarum.the effect of a pantothenic acid deficiency in lactobacillus plantarum on the initial rate of amino acid transport was investigated. although the steady-state accumulation capacity for all amino acids was markedly reduced in pantothenate-deficient cells, initial rates of uptake either were not changed (asparagine, alanine, lysine) or were increased (glutamic acid, aspartic acid leucine). the findings suggest that a reduction in membrane lipid content heterogeneously affects the operation and/or ...19751125249
oxygen metabolism of catalase-negative and catalase-positive strains of lactobacillus plantarum.two catalase-negative strains of lactobacillus plantarum and a strain producing the atypical, nonheme catalase were studied to determine if the ability to produce the atypical catalase conferred any growth advantage upon the producing strain. both catalase-negative strains grew more rapidly than the catalase-positive strain under aerobic or anaerobic conditions in a glucose-containing, complex medium. upon exhaustion of glucose from the medium, all three strains continued growth under aerobic bu ...19751141195
effect of inhibition of protein synthesis on lipid metabolism in lactobacillus lactobacillus plantarum 17-5, lipid synthesis appears to be correlated with protein synthesis. inhibition of protein synthesis by chloramphenicol (50 mug/ml) caused the nearly simultaneous inhibition of incorporation of radioactive oleic acid into polar lipids before the cessation of growth. in addition, de novo fatty acid synthesis, as determined by the incorporation of radioactive acetate into cellular lipids, was also inhibited. removal of the antibiotic resulted in the resumption of growt ...19751158852
the nature of the multiple forms of d-erythrodihydroneopterin triphosphate synthetase.1. three forms of the lactobacillus plantarum enzyme d-erythro-dihydroneopterin triphosphate synthetase, the first enzyme in folate biosynthesis, have been demonstrated by polyacrylamide gel electrophoresis. the enzyme forms designated the alpha prime, alpha and beta forms have been shown to be conformers with molecular weights of approx. 200 000. study of the subunit structure of the beta enzyme species by sodium dodecylsulfate-polyacrylamide gel electrophoresis revealed a single protein with a ...19751174547
immunoadjuvant activities of cell walls and their water-soluble fractions prepared from various gram-positive bacteria.the cell walls from all 21 species of gram-positive bacteria examined, except lysozyme-susceptible micrococcus lysodeikticus (nctc 2665) and lysozyme-resistant staphylococcus epidermidis (atcc 155), were found to be definitely adjuvant-active in both stimulation of increased serum antibody levels and induction of delayed-type hypersensitivity to ovalbumin when administered to guinea-pigs as water-in-oil emulsions. using various cell wall lytic enzymes, the immunoadjuvant principles were solubili ...19751180872
immunoadjuvant activities of peptidoglycan subunits from the cell walls of staphyloccus aureus and lactobacillus plantarum.attempts were made to isolate and identify the unit chemical structure essential for manifestation of the immunoadjuvant activities characteristic of bacterial cell walls. the n-acetylmuramyl-peptide subunit monomers, nalpha-(n-acetylmuramyl-l-alanyl-d-isoglutaminyl)-nepsilon-(glycylglycyl)-l-lysyl-d-alanine from the cell walls of staphylococcus aureus (fda 209p) and n-acetylmuramyl-l-alanyl-d-isoglutaminyl-meso-diaminopimelic acid and/or n-acetylmuramyl-l-alanyl-d-isoglutaminyl-meso-diaminopime ...19751180873
the reduction of n-hydroxy-4-acetylaminobiphenyl by the intestinal microflora of the rat.the role of the intestinal flora in the conversion of n-hydroxy-4-acetyl-aminobiphenyl (n-oh-aabp) to 4-acetylaminobiphenyl has been examined. this reaction, which reverses the metabolic activation of the parent carcinogen, can be demonstrated in cultures of some bacteria indigenous to the intestinal microflora. these include cultures of clostridium sp., clostridium perfringens, peptostreptococcus productus i, and bacteroides fragilis ss. thetaiotaomicron and ss. vulgatus. in contrast, cultures ...19751182690
antimetabolites of pantothenic acid, ureido- and carbamoyl-derivatives.pantothenic acid analogs have been synthesized that contain alkyl and/or arylureido and carbamate functions in the beta-alanyl portion of the amide moiety of the vitamin. the analogs are inhibitory to growth of lactic acid bacteria at concentrations as low as 0.6 mug/ml, and the inhibitions are competitively reversed by supplements of pantothenic acid. the carbamate derivatives are more toxic to growth of lactobacillus plantarum 8014 and pediococcus cerevisiae 8042 than the ureido analogs, and c ...19751228973
phosphopantothenic acid--the major product of pantothenic acid accumulation and a significant growth stimulatn in lactobacillus plantarum.non-proliferating cells of lactobacillus plantarum accumulated about 1.3 mug of pantothenic acid (pa) per milligram dry cells when placed in a phosphate-buffered solution containing glucose, nacl, and pa for 21 h. under the conditions applied for pa uptake, ph 7.5 and 38 degrees c, the accumulated pa existed almost exclusively in a bound form. free pa was observed only occasionally and then at questionably significant levels. all bound pa was released quantitatively by enzyme hydrolysis with alk ...19761252995
stability and reconstitution of pyruvate oxidase from lactobacillus plantarum: dissection of the stabilizing effects of coenzyme binding and subunit interaction.pyruvate oxidase from lactobacillus plantarum is a homotetrameric flavoprotein with strong binding sites for fad, tpp, and a divalent cation. treatment with acid ammonium sulfate in the presence of 1.5 m kbr leads to the release of the cofactors, yielding the stable apoenzyme. in the present study, the effects of fad, tpp, and mn2+ on the structural properties of the apoenzyme and the reconstitution of the active holoenzyme from its constituents have been investigated. as shown by circular dichr ...19921304899
characterization of the stabilizing effect of point mutations of pyruvate oxidase from lactobacillus plantarum: protection of the native state by modulating coenzyme binding and subunit interaction.point mutations in the gene of pyruvate oxidase from lactobacillus plantarum, with proline residue 178 changed to serine, serine 188 to asparagine, and alanine 458 to valine, as well as a combination of the three single point mutations, lead to a significant functional stabilization of the protein. the enzyme is a tetrameric flavoprotein with tightly bound cofactors, fad, tpp, and divalent metal ions. thus, stabilization may be achieved either at the level of tertiary or quaternary interactions, ...19921304900
melibiose transport system in lactobacillus plantarum.lactobacillus plantarum atcc 8014 grew on melibiose at 30 c, but not at 37 c, although it grew on galactose or lactose at either temperature. atcc 8014 grown on lactose at 30 or 37 c accumulated melibiose slowly, suggesting that melibiose may partly be transported by a lactose transport system. a lactose-negative mutant, ntg 21, derived from atcc 8014 was isolated. the mutant was totally deficient in lactose transport, but retained normal melibiose transport activity. in ntg 21, the melibiose tr ...19921337134
cloning and expression of alpha-amylase from bacillus amyloliquefaciens in a stable plasmid vector in lactobacillus plantarum.lactobacillus plantarum is used in a wide range of agricultural and food fermentations. in this paper we report the introduction of alpha-amylase into the organism from bacillus amyloliquefaciens on a stable recombinant plasmid. the genetically manipulated organism grew on mrsb medium supplemented with starch and it may be a prototype for the development of lactobacilli able to use an increased range of substrates in commercial fermentations.19901366859
intraspecific protoplast fusion of lactobacillus plantarum. 19901368520
structural and functional analysis of two cryptic plasmids from lactobacillus pentosus md353 and lactobacillus plantarum atcc 8014.the dna sequences of a 2.4 kb plasmid (p353-2) from lactobacillus pentosus md353 and a 1.9 kb plasmid (p8014-2) from lactobacillus plantarum atcc 8014 show 81.5% overall similarity. both plasmids carry elements (replication protein gene, plus-origin and minus-origin of replication), which are typical of plasmids that replicate via a rolling-circle mechanism of replication (rcr). direct evidence for an rcr mechanism was obtained by showing the accumulation of single-stranded plasmid intermediates ...19921387195
cloning and overexpression of lactobacillus helveticus d-lactate dehydrogenase gene in escherichia coli.nad(+)-dependent d-lactate dehydrogenase from lactobacillus helveticus was purified to apparent homogeneity, and the sequence of the first 36 amino acid residues determined. using forward and reverse oligonucleotide primers, based on the n-terminal sequence and amino acid residues 220-215 of the lactobacillus bulgaricus enzyme [kochhar, s., hunziker, p. e., leong-morgenthaler, p. & hottinger, h. (1992) j. biol. chem. 267, 8499-8513], a 0.6-kbp dna fragment was amplified from l. helveticus genomi ...19921396685
microbiological events during commercial meat fermentations.microbiological developments during industrial meat fermentations (salami), made with and without commercial starter cultures, were followed at two factories in germany and italy. in the german product microbial growth was evident only for the first 48 h, followed by a gradual decline in numbers of most micro-organisms. the ph fell from 5.8 to 4.8 in the 28 d required for production. in italy a similar situation was seen, except that a second period of bacterial growth began around 15 d, coincid ...19921399914
unusual amino acid substitution in the anion-binding site of lactobacillus plantarum non-allosteric l-lactate lactobacillus plantarum non-allosteric l-lactate dehydrogenase (l-ldh), the highly conserved his188 residue, which is involved in the binding of an allosteric effector, fructose 1,6-bisphosphate [fru(1,6)p2], in allosteric l-ldh is uniquely substituted by an asp. the mutant l. plantarum l-ldh, in which asp188 is replaced by a his, showed essentially the same fru(1,6)p2-independent catalytic activity as the wild-type enzyme, except that the km and vmax values were slightly decreased. however, ...19921425707
accelerated natural lactic fermentation of cereal-based formulas at reduced water required to dehydrate fermented cereal-based food formulas plays a significant part in the production cost. therefore the effect of reduced moisture content on the fermentation was investigated. generally, lactic fermentation at reduced moisture content resulted in increased final ph. significant acidification still occurred at 0.33 kg water/kg dry matter corresponding to either aw = 0.925 in a sorghum-maize-soya (sms) mixture, or aw = 0.950 in a sorghum-maize-milk powder (smm) mixture. a ...19921457290
cloning and expression of a conjugated bile acid hydrolase gene from lactobacillus plantarum by using a direct plate assay.the conjugated bile acid hydrolase gene from the silage isolate lactobacillus plantarum 80 was cloned and expressed in escherichia coli mc1061. for the screening of this hydrolase gene within the gene bank, a direct plate assay developed by dashkevicz and feighner (m. p. dashkevicz and s. d. feighner, appl. environ. microbiol. 53:331-336, 1989) was adapted to the growth requirements of e. coli. because of hydrolysis and medium acidification, hydrolase-active colonies were surrounded with big hal ...19921476424
energy conservation in malolactic fermentation by lactobacillus plantarum and lactobacillus sake.a comparably poor growth medium containing 0.1% yeast extract as sole non-defined constituent was developed which allowed good reproducible growth of lactic acid bacteria. of seven different strains of lactic acid bacteria tested, only lactobacillus plantarum and lactobacillus sake were found to catalyze stoichiometric conversion of l-malate to l-lactate and co2 concomitant with growth. the specific growth yield of malate fermentation to lactate at ph 5.0 was 2.0 g and 3.7 g per mol with l. plan ...19921510572
cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from lactobacillus paracasei subsp. paracasei ncdo 151.the gene encoding the cell-envelope-associated proteinase of lactobacillus paracasei subsp. paracasei ncdo 151 (formerly lactobacillus casei ncdo 151) was cloned and sequenced. the gene was located on the chromosome and encoded a polypeptide of 1902 amino acids. the proteinase is n-terminally cleaved upon maturation. it shows extensive homology to the lactococcus lactis subsp. cremoris wg2 proteinase. similar to the situation in lactococcus, a maturation gene was found upstream of the proteinase ...19921512565
mobilization and location of the genetic determinant of chloramphenicol resistance from lactobacillus plantarum catc2r.the mobilization of a nonconjugative plasmid (pcat) that mediates chloramphenicol resistance in lactobacillus plantarum catc2r was achieved by comobilization with the conjugative plasmid pam beta 1. the conjugation studies confirmed that the 8.5-kb pcat in l. plantarum catc2r contains the gene responsible for chloramphenicol resistance and that the plasmid has several unique restriction sites which make it useful for genetic studies in carnobacterium spp. cloning studies showed that the gene res ...19921513874
optimization of culture conditions for toxin production of type g clostridium botulinum.culture conditions were optimized for toxin production of clostridium botulinum type g, the last toxigenic serotype described. six factorial experiments were performed to assess the effect of medium composition (nutrients, metal ions, sterile soil, ph), incubation conditions (time, temperature and anaerobiosis) and associated microorganisms (bacillus subtilis, lactobacillus plantarum) on the toxinogenesis of c. botulinum type g. a significant (4 to 10 fold) improvement of toxin production was ob ...19921520974
novel paired starter culture system for sauerkraut, consisting of a nisin-resistant leuconostoc mesenteroides strain and a nisin-producing lactococcus lactis strain.nisin-resistant leuconostoc mesenteroides nck293 and nisin-producing lactococcus lactis subsp. lactis nck401 were evaluated separately and in combination for growth and nisin production in a model sauerkraut fermentation. strains were genetically marked and selectively enumerated by using antibiotic-containing media. the growth and survival of l. mesenteroides were similar in the presence and absence of lactococcus lactis subsp. lactis. the growth of lactococcus lactis subsp. lactis was not inhi ...19921622215
headspace flavour compounds produced by yeasts and lactobacilli during fermentation of preferments and bread doughs.production of volatile flavour compounds during fermentation with pure cultures of saccharomyces cerevisiae and candida guilliermondii, lactobacillus brevis and lactobacillus plantarum have been investigated, using wheat doughs and several preferements as substrates. for yeast, preferments consisted of 10% (w/v) glucose, maltose and sucrose solutions, whereas for lactobacilli they consisted of supplemented and unsupplemented (3% and 10% (w/v)) glucose solutions, and a 10% (w/v) wheat flour slurr ...19921622751
monoclonal antibody-colony immunoblot method specific for isolation of pediococcus acidilactici from foods and correlation with pediocin (bacteriocin) production.balb/c mice were immunized with broken, heat-killed cells of pediococcus acidilactici h. after murine cell fusions, one monoclonal antibody (mab), ped-2b2, was selected on the basis of its positive reaction with seven of seven strains tested in an enzyme-linked immunosorbent assay with whole cells of p. acidilactici. the mab ped-2b2 did not show any cross-reactions with other lactic-acid bacteria or other gram-positive or gram-negative organisms. sodium dodecyl sulfate-polyacrylamide gel electro ...19921637167
hydrolysis of vicine and convicine from fababeans by microbial beta-glucosidase enzymes.the toxic glycosides vicine and convicine which are present in fababeans have been implicated in favism, an anaemic disease of humans. vicine and convicine concentrations are reduced by growth of lactobacillus plantarum on fababean suspensions. the glycosides are eliminated from the fababean substrate by the growth of the filamentous fungus fusarium graminearum. incubation of fababean suspension with concentrated culture filtrate of aspergillus oryzae, induced for extracellular beta-glucosidase ...19921644702
spontaneous mutations changing the raffinose metabolism of lactobacillus plantarum.lactobacillus plantarum atcc 8014 grew poorly on raffinose agar plates, but large mutant colonies appeared in high frequency from a thin film of background growth. the alpha-galactosidase and beta-galactosidase activities of l. plantarum atcc 8014 and a mutant strain were studied in static cultures and ph-controlled fermenter cultures. both alpha-galactosidase and beta-galactosidase production were inducible in the parental strain; the induction was not needed in the mutant. the alpha-galactosid ...19911666502
[the range of antigenic specificity of bifidobacterium peptidoglycan].the antigenic relationships of bifidobacterium bifidum 1 peptidoglycans with different strains of this species (lva-3, 791, go-4), bifidobacteria of other species (b. adolescentis go-13, b. breve 79-38, b. lactentis 79-41, b. longum go-3) and bacteria of remote taxonomic groups (streptococcus faecalis 6-3. staphylococcus aureus com 885, s. epidermidis com 2124. lactobacillus plantarum 1, escherichia coli m-17) were studied on the basis of a highly sensitive test system permitting the registratio ...19911716036
plasmid profiles and curing of plasmids in lactobacillus plantarum strains isolated from green olive fermentations.plasmid profiles of 35 lactobacillus plantarum strains isolated from different green olive fermentors were obtained. a large number of plasmids in the ccc form (from 5 to 16) were present in all the tested strains as confirmed by a second dimension electrophoresis of dna. these plasmids, all of which remain cryptic, ranged from 2.0 to 68 kb in size. novobiocin, sodium dodecyl sulphate and ethidium bromide were used as plasmid-curing agents but only novobiocin induced loss of extrachromosomal dna ...19911761434
the influence of lactobacillus plantarum culture inoculation on the fate of staphylococcus aureus and salmonella typhimurium in montasio cheese.the growth and survival of staphylococcus aureus and salmonella typhimurium were investigated during the manufacturing and ripening of raw milk montasio cheese. initial inoculated populations in the cheese milk were about 10(5) cfu/ml for s. aureus and 10(6) cfu/ml for s. typhimurium. samples of curds and cheeses were taken during manufacturing and storage and analysed for ph and microbial populations. s. aureus increased slightly in number during the early period of ripening and attained a popu ...19911777389
bacteriocin-mediated inhibition of clostridium botulinum spores by lactic acid bacteria at refrigeration and abuse temperatures.the bacteriocinogenicity of lactococcus lactis atcc 11454, pediococcus pentosaceus atcc 43200, p. pentosaceus atcc 43201, lactobacillus plantarum bn, l. plantarum lb592, l. plantarum lb75, and lactobacillus acidophilus n2 against clostridium botulinum spores at 4, 10, 15, and 35 degrees c was investigated by modified deferred and simultaneous antagonism methods. all the strains, except l. acidophilus n2, produced inhibition zones on lawns of c. botulinum spores at 30 degrees c. l. plantarum bn, ...19911785919
[the fibronectin binding of salmonellae studied by a passive hemagglutination method].the study has shown that the fibronectin-binding properties of different salmonella species can be tested by means of passive hemagglutination with fibronectin-sensitized formulated sheep red blood cells (srbc). s. enteritidis and s. typhimurium isolated from foodstuffs, as well as the representatives of the species s. kottbus, s. anatum, s. tshiongwe and s. newlands, are capable of binding fibronectin. fibronectin-binding properties have also been detected in shigella sonnei, as well as in eubi ...19911811395
d-lactate dehydrogenase is a member of the d-isomer-specific 2-hydroxyacid dehydrogenase family. cloning, sequencing, and expression in escherichia coli of the d-lactate dehydrogenase gene of lactobacillus plantarum.the gene encoding d-lactate dehydrogenase (d-lactate: nad+ oxidoreductase, ec of lactobacillus plantarum has been sequenced, and expressed in escherichia coli cells with an inducible expression plasmid, in which the 5'-noncoding region of the gene was replaced with the tac promoter. comparison of the sequence of d-lactate dehydrogenase with l-lactate dehydrogenases, including the l. plantarum l-lactate dehydrogenase, showed no significant homology. in contrast, the d-lactate dehydrogen ...19911840590
inhibitory and stimulatory effects of cumin, oregano and their essential oils on growth and acid production of lactobacillus plantarum and leuconostoc mesenteroides.cumin at concentrations of 0.5, 1.0 and 2.0% (w/w) stimulated growth and acid production of lactobacillus plantarum and leuconostoc mesenteroides in a liquid medium. essential oil from cumin at high concentrations (300 and 600 ppm) inhibited growth and acid production of lactobacillus plantarum. after a certain period, growth of leuconostoc mesenteroides was observed at all concentrations (150, 300 and 600 ppm) and its acid production was stimulated at 600 ppm. oregano and its essential oil at a ...19911863531
secretion of tem beta-lactamase with signal sequences isolated from the chromosome of lactococcus lactis subsp. lactis.with tem beta-lactamase as a reporter gene, a set of expression-secretion-promoting fragments were isolated from the chromosome of lactococcus lactis subsp. lactis. the fact that only translocated beta-lactamase renders cells resistant to ampicillin allowed direct ampicillin selection with an escherichia coli vector (pkth33). the clones showing the greatest ampicillin resistance were subcloned onto a replicon capable of replication in lactic acid bacteria (pvs2), and the nucleotide sequences of ...19911901704
growth of bacillus cereus in fermenting tempeh made from various beans and its inhibition by lactobacillus plantarum.counts of bacillus cereus reached ca 10(8) cfu/g within 40 h in fermenting unacidified horsebean tempeh and resulted in complete spoilage of the product. in fermenting unacidified pea, chickpea and soybean tempeh, b. cereus counts reached 10(6)-10(7) cfu/g, although the products were not spoiled. inoculation of these unacidified beans with lactobacillus plantarum decreased the final count of b. cereus by 2 log units, but had no effect on its growth in unacidified horsebean tempeh and its subsequ ...19911905283
the occurrence and growth of microorganisms during the fermentation of fish sausage.minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ...19911909546
electrogenic l-malate transport by lactobacillus plantarum: a basis for energy derivation from malolactic fermentation.l-malate transport in lactobacillus plantarum was inducible, and the ph optimum was 4.5. malate uptake could be driven by an artificial proton gradient (delta ph) or an electroneutral lactate efflux. because l-lactate efflux was unable to drive l-malate transport in the absence of a delta ph, it did not appear that the carrier was a malate-lactate exchanger. the kinetics of malate transport were, however, biphasic, suggesting that the external malate concentration was also serving as a driving f ...19911917854
use of dna probes in the study of silage colonization by lactobacillus and pediococcus strains.a technique to monitor lactic acid bacteria inoculants in silage, based on specific dna probes, was developed and used to evaluate the colonization properties of two strains of lactobacillus plantarum and one strain of pediococcus pentosaceus which were used as maize silage inoculants in farm conditions. the results indicated that these three strains were able to dominate the natural microflora of the silage, representing more than the 95% of the bacterial biomass of the maize silage. these stud ...19911960106
single-stranded dna plasmid, vector construction and cloning of bacillus stearothermophilus alpha-amylase in lactobacillus.vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to taqi-digested dna of a cryptic plasmid from lactobacillus plantarum. the minimal region of lactobacillus plasmid dna that was required for dna replication was defined and a single-stranded dna intermediate replication system was observed. homologies with other origins of replication of plasmids from gram-positive bacteria, replicating via rolling circle mechanism, were found. it was shown that the c ...19911961976
purification of ferrichrome synthetase from aspergillus quadricinctus and characterisation as a phosphopantetheine containing multienzyme complex.aspergillus quadricinctus was grown under iron limitation to induce the enzymes for ferrichrome biosynthesis. the mycelium was disintegrated by ultraturrax homogenization, and ferrichrome synthetase was purified by column chromatography on deae cellulose, hydroxyapatite and bio-gel a-5m. the enzyme was almost homogeneous in single fractions as shown in gel electrophoresis under non-denaturating conditions. by fast-protein liquid chromatography on superose 6, the purified ferrichrome synthetase ( ...19911986785
ecology of accelerated natural lactic fermentation of sorghum-based infant food formulas.accelerated natural lactic fermentation of mixed sorghum-cowpea (formula 1) and sorghum-milkpowder (formula 2) infant food formulas was achieved by repetitive (60 fermentation cycles of 24 h each) use of the previous batch as an inoculum at a rate of 10% (w/w) and resulted in a gradual establishment of mixed populations of lactic acid bacteria and yeasts. in formula 1, early fermentation stages (fermentation cycles 1-4) were dominated by leuconostoc and lactococcus spp. which caused conditions i ...19912049286
trophic relationships between saccharomyces cerevisiae and lactobacillus plantarum and their metabolism of glucose and citrate.glucose and citrate are two major carbon sources in fruits or fruit juices such as orange juice. their metabolism and the microorganisms involved in their degradation were studied by inoculating with an aliquot of fermented orange juice a synthetic model medium containing glucose and citrate. at ph 3.6, their degradation led, first, to the formation of ethanol due to the activity of yeasts fermenting glucose and, eventually, to the formation of acetate resulting from the activity of lactobacilli ...19912059031
bioenergetic consequences of catabolic shifts by lactobacillus plantarum in response to shifts in environmental oxygen and ph in chemostat cultures.proton motive force (pmf), intracellular end product concentrations, and atp levels were determined when a steady-state lactobacillus plantarum 8014 anaerobic chemostat culture was shifted to an aerobic condition or was shifted from ph 5.5 to 7.5. the pmf and intracellular atp levels increased immediately after the culture was shifted from anaerobic to aerobic conditions. the concentrations of intracellular lactate and acetate, which exported protons that contributed to the proton gradient, chan ...19912066338
microbiology of 'obiolor': a nigerian fermented non-alcoholic beverage.obiolor is an acidic non-alcoholic beverage prepared by fermenting sorghum and millet malts. the traditional process for the production and microbiological characteristics of the beverage were investigated. bacillus spp., lactobacillus plantarum and streptococcus lactis were the associated micro-organisms most actively involved. yeasts were present in low numbers towards the end of the fermentation. other micro-organisms isolated did not appear to play a role in the fermentation process. variati ...19902123172
[preparation of microbial silage from fish of underutilized species].fish silage was produced by microbial means from a mixture of several fish species which are not used for human consumption, and form part of the shrimp by-catch. the fish was mixed with a carbohydrate source (molasses) and a starter culture of lactobacillus plantarum 8014. the mixture was fermented at 30 +/- 2 degrees c. several tests were undertaken to determine the optimal conditions and concentration of molasses (5, 10 and 15%) and lactobacillus (0.5, 1.0, 2.5, 5.0 and 10.0%). results indica ...19902134144
inhibition of proteolysis at harvest using heat in alfalfa silages: effects on silage composition and digestion by sheep.heat treatment at harvest was used to investigate the effects of proteolysis on silage composition and digestion by sheep. four alfalfa (medicago sativa) silages were prepared, two from mid-bloom and two from pre-bloom crops from the same field. mid-bloom alfalfa was conserved with formic acid as two unwilted silages, either without (unwilted control; uwc) or after heat treatment (unwilted heated; uwh) applied as steam for 1 min. pre-bloom alfalfa was ensiled either after 24 h wilting (wilted co ...19902180876
cloning and structure of the pyre gene of lactobacillus plantarum ccm 1904.the pyre gene of lactobacillus plantarum ccm 1904, coding for the orotate phosphoribosyl transferase involved in the pyrimidine biosynthetic pathway, was cloned in escherichia coli and sequenced. the predicted polypeptide sequence extending over 212 amino acids (mw 22,690) was compared to those of e. coli and to those of lower eukaryotes (saccharomyces cerevisiae, podospora anserina, sordaria macrospora, dictyostelium discoideum). important conserved stretches were revealed, implying that these ...19902210335
[site-specific endonucleases lpli and aagi].new site-specific endonucleases lpli and aagi have been isolated from the lactobacillus plantarum and achromobacter agile cells, respectively. the enzymes' purification stages included treatment of cell-free extracts with polyethylenimine, fractionation in two-phase system by albertsson's method, chromatography on blue sepharose and deae-cellulose. the results of cleavage of a 5'-32p-labelled oligodeoxynucleotide duplex by restriction endonucleases lpli and aagi indicate that these enzymes recog ...19902285421
manganese as a controlling factor in mixed cultures of lactobacillus plantarum and enterobacteriaceae.addition of manganese, at levels of 50 ppm, to a liquid growth medium simulating adverse silage conditions had no effect on the growth or on the fermentation pattern of enterobacter cloacae and proteus vulgaris. yet, the manganese strongly enhanced the growth of lactobacillus plantarum. co-cultures of l. plantarum and e. cloacae or p. vulgaris were, by addition of manganese ions, significantly altered in the favour of the former. this finding can be of use in mixed cultures where enterobacteriac ...19902321928
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