| structural and functional analysis of two cryptic plasmids from lactobacillus pentosus md353 and lactobacillus plantarum atcc 8014. | the dna sequences of a 2.4 kb plasmid (p353-2) from lactobacillus pentosus md353 and a 1.9 kb plasmid (p8014-2) from lactobacillus plantarum atcc 8014 show 81.5% overall similarity. both plasmids carry elements (replication protein gene, plus-origin and minus-origin of replication), which are typical of plasmids that replicate via a rolling-circle mechanism of replication (rcr). direct evidence for an rcr mechanism was obtained by showing the accumulation of single-stranded plasmid intermediates ... | 1992 | 1387195 |
| the glucose kinase gene of streptomyces coelicolor a3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression. | mutants (glk) of streptomyces coelicolor a3(2) that are resistant to the non-utilizable glucose analogue 2-deoxyglucose are deficient in glucose kinase activity, defective in glucose repression, and usually unable to utilize glucose. a 2.9 kb bcli fragment, previously shown to restore a wild-type phenotype to a glk deletion mutant that lacks the entire segment, contains two complete open reading frames that would encode proteins of 20.1 kda (orf2) and 33.1 kda (orf3). orf3 is transcribed from it ... | 1992 | 1435260 |
| organization and characterization of three genes involved in d-xylose catabolism in lactobacillus pentosus. | a cluster of three genes involved in d-xylose catabolism (viz. xylose genes) in lactobacillus pentosus has been cloned in escherichia coli and characterized by nucleotide sequence analysis. the deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in bacillus subtilis (58%), to e. coli and b. subtilis d-xylose isomerase (68% and 77%, respectively), and to e. coli d-xylulose kinase (58%). the cloned genes repres ... | 1991 | 1660563 |
| complementation of the inability of lactobacillus strains to utilize d-xylose with d-xylose catabolism-encoding genes of lactobacillus pentosus. | the inability of two lactobacillus strains to ferment d-xylose was complemented by the introduction of lactobacillus pentosus genes encoding d-xylose isomerase, d-xylulose kinase, and a d-xylose catabolism regulatory protein. this result opens the possibility of using d-xylose fermentation as a food-grade selection marker for lactobacillus spp. | 1991 | 1768150 |
| heme-dependent catalase activity of lactobacilli. | the heme-dependent catalase in lactobacillus pentosus, l. sake, l. delbrueckii and enterococcus faecalis was studied. the catalase was formed by cells grown aerobically in the presence of hematin or for lactobacilli when grown without added hematin, after incubation of buffered cells in the presence of hematin. the kinetics of the production of catalase revealed maximum activity for l. pentosus and e. faecalis at late stationary and late logarithmic growth phase, respectively. the physiological ... | 1991 | 1904757 |
| control of replication of the lactobacillus pentosus plasmid p353-2: evidence for a mechanism involving transcriptional attenuation of the gene coding for the replication protein. | the synthesis of plasmid dna and of rna encoded by the replication protein gene (rep) of plasmid p353-2 of lactobacillus pentosus was studied for the wild-type plasmid and for a mutant plasmid with a deletion in the 5' untranslated region of the rep gene. plasmid p353-2 codes for two countertranscript rnas (ct-rna) of approximately 75 and 250 nucleotides transcribed from the 5' untranslated region of the rep gene, in opposite directions. in a mutant plasmid with a deletion of the promoter and pa ... | 1994 | 7510019 |
| influence of ph, salt and nitrite on the heme-dependent catalase activity of lactic acid bacteria. | a screening of commercial starter cultures used for the production of dry sausage showed a maximum heme-dependent catalase activity in the range of 60 mumol/l hematin for lactobacillus sake, lactobacillus plantarum, lactobacillus pentosus and pediococcus acidilactici. pseudocatalase activity was not detected. in standard dry sausage production, 2-3% (w/w) nitrite salt (0.6% sodium nitrite per 100 g nacl) is normally added, which corresponds to 4-6% salt in the water phase. in vitro experiments w ... | 1994 | 7703013 |
| promoter analysis and transcriptional regulation of lactobacillus pentosus genes involved in xylose catabolism. | the xyl genes in lactobacillus pentosus are induced by xylose and repressed by glucose, ribose, and arabinose. northern blot analysis showed that regulation is mediated at the transcriptional level. under inducing conditions, two xyla transcripts were detected, a major transcript of 1.5 kb and a minor transcript of 3 kb. the 3 kb transcript also comprises sequences from xylb, suggesting that xyla and xylb are transcribed together. a 1.2 kb xylr transcript was found under inducing and non-inducin ... | 1994 | 7845354 |
| transcription of the xyl operon is controlled in bacillus subtilis by tandem overlapping operators spaced by four base-pairs. | the expression of xylose utilization in bacillus subtilis is regulated at the level of transcription by xylose dependent xyl repressor-xyl operator interaction. we have structurally and functionally characterized the binding sites of xyl repressor in the xyl regulatory region. methylation and hydroxyl radical protection and ethylation interference of binding suggests tandem overlapping xyl operators spaced by four base-pairs. a mutational inactivation of each and both operators was performed. dn ... | 1994 | 7966270 |
| lactobacillus plantarum ldhl gene: overexpression and deletion. | lactobacillus plantarum is a lactic acid bacterium that converts pyruvate to l-(+)- and d-(-)-lactate with stereospecific enzymes designated l-(+)- and d-(-)-lactate dehydrogenase (ldh), respectively. a gene (designated ldhl) that encodes l-(+)-lactate dehydrogenase from l. plantarum dg301 was cloned by complementation in escherichia coli. the nucleotide sequence of the ldhl gene predicted a protein of 320 amino acids closely related to that of lactobacillus pentosus. a multicopy plasmid bearing ... | 1994 | 8300514 |
| insights into substrate binding by d-2-ketoacid dehydrogenases from the structure of lactobacillus pentosus d-lactate dehydrogenase. | d-lactate dehydrogenases (d-ldhs) and l-lactate dehydrogenases (l-ldhs) catalyze a reaction differing only in the chirality of the product. both enzymes utilize the same kind of amino acid side chains in substrate binding and catalysis. models based on d-ldh-related enzymes propose that these side chains assume identical roles in both enzymes with their active sites related by a simple geometrical relationship such as a mirror plane. | 1996 | 8740366 |
| evaluation of numerical analysis of random amplified polymorphic dna (rapd)-pcr as a method to differentiate lactobacillus plantarum and lactobacillus pentosus. | lactobacillus plantarum and lactobacillus pentosus grouped into one protein profile cluster at r > or = 0.70, separate from lactobacillus casei, lactobacillus sake, and lactobacillus curvatus. similar sugar fermentation reactions were recorded for representative strains of l. plantarum and l. pentosus. representative strains, including the type of each species, were selected from the different protein profile clusters and their genetic relatedness determined by using numerical analysis of random ... | 1996 | 8867459 |
| inducible gene expression and environmentally regulated genes in lactic acid bacteria. | relatively recently, a number of genes and operons have been identified in lactic acid bacteria that are inducible and respond to environmental factors. some of these genes/operons had been isolated and analysed because of their importance in the fermentation industry and, consequently, their transcription was studied and found to be regulatable. examples are the lactose operon, the operon for nisin production, and genes in the proteolytic pathway of lactococcus lactis, as well as xylose metabol ... | 1996 | 8879404 |
| characterization of lactobacilli by southern-type hybridization with a lactobacillus plantarum pyrdfe probe. | lactobacillus plantarum, lactobacillus pentosus, and lactobacillus paraplantarum (m.-c. curk, j.-c. hubert, and f. bringel, int. j. syst. bacteriol. 46:595-598, 1996) can hardly be distinguished on the basis of their phenotypes. unlike l. plantarum and l. paraplantarum, l. pentosus ferments glycerol and xylose but not melezitose. we identified two l. pentosus strains (cnrz 1538 and cnrz 1544) which ferment glycerol and melezitose but not xylose. alpha-methyl-d-mannoside was fermented by 66% of t ... | 1996 | 8934911 |
| mathematical description of the growth of lactobacillus sake and lactobacillus pentosus under conditions prevailing in fermented sausages. | the growth behaviour of lactobacillus sake and lactobacillus pentosus was determined in a model system stimulating the conditions of fermenting sausages. minor effects on the growth were observed by varying the concentrations of glucose, peptone, manganese and sodium nitrite. temperature and sodium chloride concentration were found to have most effect on the growth. the measured data were used for a mathematical model describing the growth response of l. sake and l. pentosus sufficiently well to ... | 1996 | 8987721 |
| regulation of expression of the lactobacillus pentosus xylab operon. | the xylose cluster of lactobacillus pentosus consists of five genes, two of which, xylab, form an operon and code for the enzymes involved in the catabolism of xylose, while a third encodes a regulatory protein, xylr. by introduction of a multicopy plasmid carrying the xyl operator and by disruption of the chromosomal xylr gene, it was shown that l. pentosus xylr encodes a repressor. constitutive expression of xylab in the xylr mutant is repressed by glucose, indicating that glucose repression d ... | 1997 | 9286992 |
| involvement of glu-264 and arg-235 in the essential interaction between the catalytic imidazole and substrate for the d-lactate dehydrogenase catalysis. | for lactobacillus pentosus d-lactate dehydrogenase, the binding of 2-ketoacids is markedly stabilized through interactions between the protonated imidazole of his-296, an acid/base catalyst of the enzyme, and the carbonyl oxygen of 2-ketoacids. the replacement of arg-235 with gln destabilized the inhibitory binding of oxamate much more than that of formate, acetate, or propionate, and the arg to lys substitution specifically diminished only oxamate binding. on the other hand, replacement of a co ... | 1997 | 9399585 |
| rapid species identification within two groups of closely related lactobacilli using pcr primers that target the 16s/23s rrna spacer region. | a rapid and reliable pcr-based method for distinguishing closely related species within two groups of lactobacilli is described. primers complementary to species-specific sequences in the 16s/23s rdna spacer regions were designed after sequencing and sequence comparison of the spacer regions of 32 strains. the strains belong to two groups of closely related lactobacillus species; one composed of lactobacillus curvatus, lactobacillus graminis and lactobacillus sake, the other of lactobacillus par ... | 1998 | 9561736 |
| cloning, sequence analysis, and characterization of the genes involved in isoprimeverose metabolism in lactobacillus pentosus. | two genes, xylp and xylq, from the xylose regulon of lactobacillus pentosus were cloned and sequenced. together with the repressor gene of the regulon, xylr, the xylpq genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylp. a putative xylr binding site (xylo) and a cre-like element, mediating ccpa-dependent catabolite repression, were found in the promoter region. l. pentosus mutants in which both xylp and xylq (lpe1) or only xy ... | 1998 | 9573180 |
| functional expression in lactobacillus plantarum of xylp encoding the isoprimeverose transporter of lactobacillus pentosus. | the xylp gene of lactobacillus pentosus, the first gene of the xylpqr operon, was recently found to be involved in isoprimeverose metabolism. by expression of xylp on a multicopy plasmid in lactobacillus plantarum 80, a strain which lacks active isoprimeverose and d-xylose transport activities, it was shown that xylp encodes a transporter. functional expression of the xylp transporter was shown by uptake of isoprimeverose in l. plantarum 80 cells, and this transport was driven by the proton moti ... | 1998 | 9683504 |
| molecular cloning and functional expression in lactobacillus plantarum 80 of xylt, encoding the d-xylose-h+ symporter of lactobacillus brevis. | a 3-kb region, located downstream of the lactobacillus brevis xyla gene (encoding d-xylose isomerase), was cloned in escherichia coli tg1. the sequence revealed two open reading frames which could code for the d-xylulose kinase gene (xylb) and another gene (xylt) encoding a protein of 457 amino acids with significant similarity to the d-xylose-h+ symporters of e. coli, xyle (57%), and bacillus megaterium, xylt (58%), to the d-xylose-na+ symporter of tetragenococcus halophila, xyle (57%), and to ... | 1998 | 9835554 |
| characterization of lactic acid bacteria isolated from a thai low-salt fermented fish product and the role of garlic as substrate for fermentation. | lactic acid bacteria (lab) isolated from raw materials (fish, rice, garlic and banana leaves) and processed som-fak (a thai low-salt fermented fish product) were characterized by api 50-ch and other phenotypic criteria. lactococcus lactis subsp. lactis and leuconostoc citreum were specifically associated with fish fillet and minced fish, lactobacillus paracasei subsp. paracasei with boiled rice and weisella confusa with garlic mix and banana leaves. in addition, lactobacillus plantarum, lactobac ... | 1999 | 10100902 |
| duplication of the beta-galactosidase gene in some lactobacillus plantarum strains. | curing of a plasmid that encoded a beta-galactosidase gene (beta-gal) from the lactobacillus plantarum strain of dairy origin ll441 was not accompanied by complete loss of the lactose utilization phenotype. dna-dna hybridization, using an internal fragment of the beta-gal gene as a probe, revealed a second determinant located on the chromosome of the cured derivatives. the chromosomal copy was present in all of a series of beta-gal+ l. plantarum and lactobacillus pentosus strains from different ... | 1999 | 10426447 |
| transport of d-xylose in lactobacillus pentosus, lactobacillus casei, and lactobacillus plantarum: evidence for a mechanism of facilitated diffusion via the phosphoenolpyruvate:mannose phosphotransferase system. | we have identified and characterized the d-xylose transport system of lactobacillus pentosus. uptake of d-xylose was not driven by the proton motive force generated by malolactic fermentation and required d-xylose metabolism. the kinetics of d-xylose transport were indicative of a low-affinity facilitated-diffusion system with an apparent k(m) of 8.5 mm and a v(max) of 23 nmol min(-1) mg of dry weight(-1). in two mutants of l. pentosus defective in the phosphoenolpyruvate:mannose phosphotransfer ... | 1999 | 10438743 |
| characterization of pentocin tv35b, a bacteriocin-like peptide isolated from lactobacillus pentosus with a fungistatic effect on candida albicans. | lactobacillus pentosus tv35b, isolated from the posterior fornix secretions of the vagina of a prenatal patient, produced a bacteriocin-like peptide (pentocin tv35b), which is inhibitory to clostridium sporogenes, cl. tyrobutyricum, lact. curvatus, lact. fermentum, lact. sake, listeria innocua, propionibacterium acidipropionici, propionibacterium sp. and candida albicans. the mechanism of activity of pentocin tv35b is bactericidal, as shown by a decrease in the viable cell numbers of lact. sake ... | 1999 | 10594714 |
| carbon catabolite repression in lactobacillus pentosus: analysis of the ccpa region. | the catabolite control protein ccpa is a central regulator in low-g+c-content gram-positive bacteria. it confers carbon catabolite repression to numerous genes required for carbon utilization. it also operates as a transcriptional activator of genes involved in diverse phenomena, such as glycolysis and ammonium fixation. we have cloned the ccpa region of lactobacillus pentosus. ccpa encodes a protein of 336 amino acids exhibiting similarity to ccpa proteins of other bacteria and to proteins of t ... | 2000 | 10618236 |
| biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of 'almagro' eggplants. | a total of 149 strains of lactic acid bacteria isolated from the spontaneous fermentation of 'almagro' eggplants were characterized and identified. of the isolates, 148 were determined as belonging to the genus lactobacillus. a coccoid, gram-positive database-negative isolate was obtained in the early stages of fermentation. the lactobacillus strains were divided into six groups based on sugar fermentation patterns and other physiological and morphological characteristics, and were identified as ... | 2000 | 10946834 |
| some lactobacillus l-lactate dehydrogenases exhibit comparable catalytic activities for pyruvate and oxaloacetate. | the nonallosteric and allosteric l-lactate dehydrogenases of lactobacillus pentosus and l. casei, respectively, exhibited broad substrate specificities, giving virtually the same maximal reaction velocity and substrate k(m) values for pyruvate and oxaloacetate. replacement of pro101 with asn reduced the activity of the l. pentosus enzyme toward these alternative substrates to a greater extent than the activity toward pyruvate. | 2001 | 11114942 |
| contribution of the phosphoenolpyruvate:mannose phosphotransferase system to carbon catabolite repression in lactobacillus pentosus. | the role of the lactobacillus pentosus phosphoenolpyruvate:mannose phosphotransferase system (mannose pts) in sugar transport and control of sugar utilization was investigated. growth experiments and measurements of pep-dependent phosphorylation of sugars, of sugar transport and of catabolic enzyme activity were performed, to compare a wild-type strain with an eiib(man) mutant, lpe6, and a ccpa mutant, lpe4. fructose uptake in wild-type bacteria demonstrated the presence of two fructose-specific ... | 2001 | 11238974 |
| effect of initial glucose concentration and inoculation level of lactic acid bacteria in shrimp waste ensilation. | fermentation conditions and microorganisms were determined, based on acid production, glucose concentration as carbohydrate source. inoculation levels to obtain a stable shrimp waste silage were also determined. shrimp waste ensilation was an efficient method of preservation, allowing the recovery of chitin and another added-value products such as pigments, proteins and enzymes. from the various lactic acid bacteria tested, lactobacillus pentosus and lactobacillus sp. (b2) were the best lactic a ... | 2001 | 11240204 |
| characterization of lactobacilli from scotch malt whisky distilleries and description of lactobacillus ferintoshensis sp. nov., a new species isolated from malt whisky fermentations. | sixty-four strains of lactobacillus were isolated from fermentation samples from 23 malt whisky distilleries located in the major whisky producing regions of scotland. the strains were assigned to 26 ribotype patterns. strains of some ribotype patterns were widely distributed and recovered from distilleries throughout scotland, while strains representing other ribotypes were particular to a specific region or even a certain distillery. repeated sampling of a single distillery over a 12 month per ... | 2001 | 11283296 |
| xyloside transport by xylp, a member of the galactoside-pentoside-hexuronide family. | this paper describes the functional characterization of the xyloside transporter, xylp, of lactobacillus pentosus with the aid of a spectroscopy-based assay system. in order to monitor the transport reaction, the natural xyloside isoprimeverose, a building block of hemicellulose, and the analogue methyl-isoprimeverose were chemically synthesized by a new and efficient procedure. the xylp protein was purified by metal affinity chromatography, following high level expression in lactococcus lactis ... | 2001 | 11408491 |
| differentiation of lactobacillus plantarum, l. pentosus, and l. paraplantarum by reca gene sequence analysis and multiplex pcr assay with reca gene-derived primers. | in this study, we succeeded in differentiating lactobacillus plantarum, lactobacillus pentosus, and lactobacillus paraplantarum by means of reca gene sequence comparison. short homologous regions of about 360 bp were amplified by pcr with degenerate consensus primers, sequenced, and analyzed, and 322 bp were considered for the inference of phylogenetic trees. phylograms, obtained by parsimony, maximum likelihood, and analysis of data matrices with the neighbor-joining model, were coherent and cl ... | 2001 | 11472918 |
| utilization at high ph of starter cultures of lactobacilli for spanish-style green olive fermentation. | inoculation at alkaline ph (above 9) of lye-treated green olives with starter cultures of lactobacillus pentosus cect 5138 was studied. despite an initial loss of viability in the order of 1-2 log cycles on average, depending mainly on time of application, cultures grew and initiated an accelerated fermentation process. inoculation reduced the population of enterobacteriaceae, and thereby potential spoilage, and produced a quicker acidification of brines and decrease of ph, when compared with co ... | 2001 | 11482559 |
| efficient protoplast regeneration for some homofermentative lactobacilli and pediococci. | conditions for protoplast regeneration were examined for several strains of homofermentative lactobacilli and pediococci isolated from silage. attempts to regenerate protoplasts using previously published agar regeneration media for lactobacilli were unsuccessful for most of the strains. replacing or increasing colloidal substances in a medium containing raffinose and mgcl(2) as osmotic stabilizers enabled efficient regeneration of the protoplasts at a frequency of 10-99%. a medium containing ge ... | 2001 | 11797042 |
| lactic acid production from wheat straw hemicellulose hydrolysate by lactobacillus pentosus and lactobacillus brevis. | lactic acid production by lactobacillus brevis and lactobacillus pentosus on a hemicellulose hydrolysate (hh) of wet-oxidized wheat straw was evaluated. the potential of 11-12 g/l fermentable sugars was released from the hh through either enzymatic or acidic pretreatment. fermentation of added xylose in untreated hh after wet-oxidation, showed no inhibition on the lactic acid production by either lb. pentosus or lb. brevis. lb. pentosus produced lactate corresponding to 88% of the theoretical ma ... | 2002 | 11800488 |
| crystal structure of non-allosteric l-lactate dehydrogenase from lactobacillus pentosus at 2.3 a resolution: specific interactions at subunit interfaces. | l-lactate dehydrogenase (ldh) from lactobacillus pentosus is a non-allosteric enzyme, which shows, however, high sequence similarity to allosteric ldhs from certain bacteria. to elucidate the structural basis of the absence of allostery of l. pentosus ldh (lpldh), we determined the crystal structure of lpldh at 2.3 a resolution. bacterial ldhs are tetrameric enzymes composed of identical subunits and exhibit 222 symmetry. the quaternary structure of lpldh was similar to the active conformation o ... | 2002 | 11807949 |
| expression of the xylulose 5-phosphate phosphoketolase gene, xpka, from lactobacillus pentosus md363 is induced by sugars that are fermented via the phosphoketolase pathway and is repressed by glucose mediated by ccpa and the mannose phosphoenolpyruvate phosphotransferase system. | purification of xylulose 5-phosphate phosphoketolase (xpka), the central enzyme of the phosphoketolase pathway (pkp) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpka, from lactobacillus pentosus md363 are described. xpka encodes a 788-amino-acid protein with a calculated mass of 88,705 da. expression of xpka in escherichia coli led to an increase in xpka activity, while an xpka knockout mutant of l. pentosus lost xpka activity and was not able to grow on ener ... | 2002 | 11823225 |
| genotypic and phenotypic characterization of garlic-fermenting lactic acid bacteria isolated from som-fak, a thai low-salt fermented fish product. | to evaluate the importance of garlic for fermentation of a thai fish product, and to differentiate among garlic-/inulin-fermenting lactic acid bacteria (lab) at strain level. | 2002 | 11849359 |
| differentiation of lactobacillus plantarum, l. pentosus and l. paraplantarum species by rapd-pcr and aflp. | two high-resolution genotypic techniques (rapd-pcr and aflp) were evaluated for their possibility to discriminate the species lactobacillus plantarum, lactobacillus pentosus and lactobacillus paraplantarum and to type these taxa at the infra-species level. in total 23 strains of l. plantarum, three strains of l. pentosus, two strains of l. paraplantarum and two related strains for which the species assignment was not clear, were studied. for rapd-pcr, suitable oligonucleotides and amplification ... | 2001 | 11876363 |
| polyphasic investigation of the diversity within lactobacillus plantarum related strains revealed two l. plantarum subgroups. | the diversity of 140 strains related to lactobacillus plantarum was investigated using a polyphasic approach combining two molecular techniques: randomly amplified polymorphic dna fingerprinting (rapd) and southern hybridisation with a pyr probe on bgli digests of chromosomal dna, as well as phenotypic characterization. the rapd technique allowed us to classify a subset of 60 representative strains into four groups. one group belonged to lactobacillus paraplantarum, the second to lactobacillus p ... | 2001 | 11876364 |
| oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation. | blue native electrophoresis is used widely for the analysis of non-dissociated protein complexes with respect to composition, oligomeric state and molecular mass. however, the effects of detergent or dye binding on the mass and stability of the integral membrane proteins have not been studied. by comparison with analytical ultracentrifugation, we have evaluated whether the oligomeric state of membrane transport proteins is reflected reliably with blue native electrophoresis. for the analysis we ... | 2002 | 11955011 |
| purification and characterisation of an extracellular fructan beta-fructosidase from a lactobacillus pentosus strain isolated from fermented fish. | lactobacillus pentosus b235, which was isolated as part of the dominant microflora from a garlic containing fermented fish product, was grown in a chemically defined medium with inulin as the sole carbohydrate source. an extracellular fructan beta-fructosidase was purified to homogeneity from the bacterial supernatant by ultrafiltration, anion exchange chromatography and hydrophobic interaction chromatography. the molecular weight of the enzyme was estimated to be approximately 126 kda by gel fi ... | 2002 | 12086179 |
| lactic acid bacteria isolated from soy sauce mash in thailand. | fourteen sphere-shaped and 30 rod-shaped lactic acid bacteria were isolated from soy sauce mash of two factories in thailand. these strains were separated into two groups, group a and group b, by cell shape and dna-dna similarity. group a contained 14 tetrad-forming strains, and these strains were identified as tetragenococcus halophilus by dna similarity. group b contained 30 rod-shaped bacteria, and they were further divided into four subgroups, b1, b2, b3, and b4, and three ungrouped strains ... | 2002 | 12469319 |
| interaction between probiotic lactic acid bacteria and canine enteric pathogens: a risk factor for intestinal enterococcus faecium colonization? | selected probiotic lactic acid bacteria (lab) have been shown to elicit positive health effects particularly in humans. competitive exclusion of pathogens is one of the most important beneficial health claims of probiotic bacteria. the effect of probiotic lab on competitive exclusion of pathogens has been demonstrated in humans, chicken and pigs. in this study we evaluated the ability of certain lab strains (lactobacillus rhamnosus gg, bifidobacterium lactis bb12, lactobacillus pentosus uk1a, l. ... | 2003 | 12488075 |
| lactic acid bacteria found in fermented fish in thailand. | forty-seven strains of homofermentative rod-shaped and 5 heterofermentative sphere-shaped lactic acid bacteria were isolated from 4 kinds of fermented fish (pla-ra, pla-chom, kung-chom, and hoi-dong) in thailand. these bacteria were separated into four groups by phenotypic and chemotaxonomic characteristics, including fluorometric dna-dna hybridization. five strains (group i) contained meso-diaminopimelic acid in the cell wall. four strains were identified as lactobacillus pentosus, and one stra ... | 1998 | 12501428 |
| evolution of microbial populations during traditional feta cheese manufacture and ripening. | in three different dairies (a, b and c) located in peloponess region (southern greece), traditional feta cheese trials took place february to march using mixtures of sheep's and goat's milk. only small variations in the evolution of microbial groups were observed during the whole ripening period. the main groups, such as thermophilic cocci, mesophilic lactococci, thermophilic lactobacilli, nonstarter lactic acid bacteria (nslab), presumptive leuconostoc, enterococci and micrococci, reached their ... | 2003 | 12568755 |
| identification of lactic acid bacteria isolated from human vaginal secretions. | a total of 57 lactic acid bacteria were isolated from the vaginal secretions of 259 patients. of these strains, 37 were isolated from patients attending pre-natal clinics and the remaining strains from patients attending post-natal clinics. the strains were identified by using simple physiological and biochemical tests and their phenotypic relatedness determined by numerical analysis of total soluble cell protein patterns. the genotypic relatedness of representative strains selected from each of ... | 2003 | 12785305 |
| characterization of lactobacillus sakei by the type of stereoisomers of lactic acid produced. | lactobacillus sakei strains were characterized by the shift of the type of stereoisomers of lactic acid produced in the presence of 50 mm sodium acetate in a medium. of 27 lactobacillus sakei strains studied, 20 strains showed high levels of dna-dna similarity with l. sakei nric 1071(t), and were confirmed as l. sakei. the three remaining strains were identified as lactobacillus curvatus by dna-dna similarity, and three other strains were included in the cluster of lactobacillus plantarum/lactob ... | 2003 | 12833214 |
| bacterial community dynamics during production of registered designation of origin salers cheese as evaluated by 16s rrna gene single-strand conformation polymorphism analysis. | microbial dynamics during processing and ripening of traditional cheeses such as registered designation of origin salers cheese, an artisanal cheese produced in france, play an important role in the elaboration of sensory qualities. the aim of the present study was to obtain a picture of the dynamics of the microbial ecosystem of rdo salers cheese by using culture-independent methods. this included dna extraction, pcr, and single-strand conformation polymorphism (sscp) analysis. bacterial and hi ... | 2003 | 12839752 |
| conversion of lactobacillus pentosus d-lactate dehydrogenase to a d-hydroxyisocaproate dehydrogenase through a single amino acid replacement. | the single amino acid replacement of tyr52 with leu drastically increased the activity of lactobacillus pentosus nad-dependent d-lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by about 30-fold, converting the enzyme into a highly active d-2-hydroxyisocaproate dehydrogenase. | 2003 | 12897026 |
| the mechanism of action of lactobacillus pentosus nucleosidase. | | 1952 | 12999753 |
| effect of certain carbohydrates on nutritional requirements of lactobacillus pentosus. | | 1952 | 13047358 |
| formation of ribose phosphate from xylose by extracts of lactobacillus pentosus. | | 1953 | 13117876 |
| conversion of d-xylose to d-xylulose in extracts of lactobacillus pentosus. | | 1953 | 13117877 |
| fermentation of ribose-c14 by lactobacillus pentosus. | | 1953 | 13117910 |
| the role of xylulose 5-phosphate in xylose metabolism of lactobacillus pentosus. | | 1956 | 13295228 |
| the purification of phosphoketopentoepimerase from lactobacillus pentosus and the preparation of xylulose 5-phosphate. | | 1956 | 13385247 |
| l-ribulokinase and the formation of d-xylulose phosphate in lactobacillus pentosus. | | 1957 | 13436504 |
| observations on the mechanism of fermentation of 1-c14-d-xylose by lactobacillus pentosus. | | 1951 | 14824083 |
| studies on the specificity of the fermentation of pentoses by lactobacillus pentosus. | | 1951 | 14888645 |
| the cleavage of adenosine, cytidine, and xanthosine by lactobacillus pentosus. | | 1951 | 14917682 |
| fermentation of 1-c14-d-xylose by lactobacillus pentosus. | | 1952 | 14927647 |
| characterization of bacteriocin-like inhibitory substances (blis) from sourdough lactic acid bacteria and evaluation of their in vitro and in situ activity. | to identify and characterize bacteriocion-producing lactic acid bacteria (lab) in sourdoughs and to compare in vitro and in situ bacteriocin activity of sourdough- and nonsourdough lab. | 2004 | 14962132 |
| intra-specific variation of lactobacillus plantarum and lactobacillus pentosus in sensitivity towards various bacteriocins. | fifty-two strains belonging to the lactobacillus plantarum species group were identified and typed. they represented 32 clones of lactobacillus plantarum and 7 clones of lactobacillus pentosus. sensitivity of all strains towards bacteriocins of four different producer strains was investigated using a deferred inhibition test (dit). substantial intra-specific variation in sensitivity of clones was observed towards bacteriocinogenic lactic acid bacteria producing nisin ( lactococcus lactis atcc 11 ... | 2004 | 15028870 |
| relative ability of orally administered lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract. | five porcine-derived lactobacillus or pediococcus isolates administered to pigs (n = 4), either singly or as a combination at approximately 10(10) cfu per day varied with respect to intestinal survival and persistence. two lactobacillus murinus strains survived best and were excreted at approximately 10(7) to 10(8) cfu/g of feces. in contrast, pediococcus pentosaceus dpc6006 had the lowest fecal count at approximately 10(5) cfu/g and was excreted at a significantly lower level than both l. murin ... | 2004 | 15066778 |
| characterisation of autolytic enzymes in lactobacillus pentosus. | to characterize autolysis and autolytic system of the lactic acid bacterium lactobacillus pentosus. | 2004 | 15130139 |
| screening of the equine intestinal microflora for potential probiotic organisms. | probiotics have not been demonstrated to provide any beneficial health effects in horses, possibly because of improper selection of probiotic organisms. this study was designed to identify lactic acid bacteria of equine origin with predetermined beneficial properties which might make them useful as therapeutic probiotics. | 2004 | 15163044 |
| isolation and characterization of anti-salmonella lactic acid bacteria from the porcine gastrointestinal tract. | to identify lactic acid bacteria (lab) of porcine intestinal origin with anti-salmonella activity. | 2004 | 15482434 |
| screening of lactic acid bacteria from fermented vegetables by carbohydrate profiling and pcr-elisa. | the aim of this study was to identify potential souring agents, isolated from fermented plant material, by api 50 chl assay and a molecular method based on polymerase chain reaction and colorimetric hybridization (pcr-elisa). | 2004 | 15482435 |
| occurrence of listeria monocytogenes in green table olives. | microbiological safety of green table olives from different cultivars, prepared by both the spanish-style and biological methods and fermented with starter cultures of lactic acid bacteria (lactobacillus plantarum, lactobacillus casei, and lactobacillus pentosus), was investigated. the fermentation process was monitored by measuring ph values, titratable acidities, and growth of lactic acid bacteria over time. during fermentation, lactic acid bacteria and yeasts were major microbial populations. ... | 2004 | 15508629 |
| lactobacillus paraplantarum sp. now., a new species related to lactobacillus plantarum. | four strains of facultatively heterofermentative lactobacilli isolated from beer and human feces have physiological characteristics similar to those of lactobacillus plantarum. unlike 66% of the l. plantarum strains tested (f. bringel, m.-c. curk, and j.-c. hubert, int. j. syst. bacteriol. 46:588-594, 1996), these strains do not catabolize alpha-methyl-d-mannoside. however, because they exhibit little dna relatedness to l. plantarum and lactobacillus pentosus, these four strains were classified ... | 1996 | 15551474 |
| characterization of an acid phosphatase from lactobacillus pentosus: regulation and biochemical properties. | to screen for phosphatase and phytase activities in lactobacillus isolated from diverse ecosystems and to determine the biochemical properties and the factors that regulate the synthesis of the enzyme responsible for these activities in the selected strain, lactobacillus pentosus cect 4023. | 2005 | 15610436 |
| polyphasic study of wine lactobacillus strains: taxonomic implications. | one hundred and seventy-eight lactobacilli isolated from wine were characterized by a polyphasic approach. strains were phenotypically identified at genus and species level by classical tests including the analysis of cell morphology, homo/heterofermentative character, sugar fermentation patterns, growth at different temperatures and the optical nature of the isomer of lactic acid produced from glucose. molecular techniques such as random amplification of polymorphic dna (rapd), amplified 16s rd ... | 2005 | 15653876 |
| distinct conformation-mediated functions of an active site loop in the catalytic reactions of nad-dependent d-lactate dehydrogenase and formate dehydrogenase. | the three-dimensional structures of nad-dependent d-lactate dehydrogenase (d-ldh) and formate dehydrogenase (fdh), which resemble each other, imply that the two enzymes commonly employ certain main chain atoms, which are located on corresponding loop structures in the active sites of the two enzymes, for their respective catalytic functions. these active site loops adopt different conformations in the two enzymes, a difference likely attributable to hydrogen bonds with asn97 and glu141, which ar ... | 2005 | 15734738 |
| a beta-glucosidase gene isolated from wine lactobacillus plantarum is regulated by abiotic stresses. | little genetic information exists on the ability of wine lactic acid bacteria (lab) to hydrolyse glycoconjugates during malolactic fermentation. we tried to fill this important gap by characterizing a gene codifying for a putative beta-glucosidase enzyme from wine lactobacillus plantarum and from a commercial strain of oenococcus oeni. | 2005 | 15752331 |
| influence of the metabolism pathway on lactic acid production from hemicellulosic trimming vine shoots hydrolyzates using lactobacillus pentosus. | hemicellulosic hydrolyzates from trimming wastes of vine shoots were proposed as a carbon source for lactic acid production by lactobacillus pentosus cect-4023t (atcc-8041). these hydrolyzates are composed mainly of glucose (12.0 g/l), xylose (17.5 g/l) and arabinose (4.3 g/l). acetic acid, the main subproduct, started to be produced after all of the glucose was completely depleted, showing that the acetic acid coproduction came only from the xylose and arabinose consumption. in the absence of g ... | 2005 | 15932258 |
| rapid differentiation and in situ detection of 16 sourdough lactobacillus species by multiplex pcr. | a two-step multiplex pcr-based method was designed for the rapid detection of 16 species of lactobacilli known to be commonly present in sourdough. the first step of multiplex pcr was developed with a mixture of group-specific primers, while the second step included three multiplex pcr assays with a mixture of species-specific primers. primers were derived from sequences that specify the 16s rrna, the 16s-23s rrna intergenic spacer region, and part of the 23s rrna gene. the primer pairs designed ... | 2005 | 15933001 |
| evaluation of lactobacillus pentosus we7 for prevention of diarrhea in neonatal foals. | to evaluate the efficacy of lactobacillus pentosus we7, an equine-origin organism with potentially beneficial in vitro properties, as a probiotic for prevention of neonatal diarrhea in foals. | 2005 | 15989186 |
| bacterial population in traditional sourdough evaluated by molecular methods. | to study the microbial communities in artisanal sourdoughs, manufactured by traditional procedure in different areas of sicily, and to evaluate the lactic acid bacteria (lab) population by classical and culture-independent approaches. | 2005 | 16033455 |
| caseinoglycomacropeptide inhibits adhesion of pathogenic escherichia coli strains to human cells in culture. | caseinoglycomacropeptide (cgmp) derived from kappa-casein was investigated for its ability to inhibit the adhesion of 3 strains of verotoxigenic escherichia coli (vtec) and 3 strains of enteropathogenic escherichia coli (epec) to human ht29 tissue cell cultures. effects on adhesion of desulfovibrio desulfuricans, lactobacillus pentosus, lactobacillus casei, lactobacillus acidophilus, and lactobacillus gasseri were also investigated. generally, cgmp exerted effective anti-adhesive properties at a ... | 2005 | 16162518 |
| use of real time pcr to determine population profiles of individual species of lactic acid bacteria in alfalfa silage and stored corn stover. | real-time polymerase chain reaction (rt-pcr) was used to quantify seven species of lactic acid bacteria (lab) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. species-specific pcr primers were designed based on reca gene sequences. commercial inoculants improved the quality of alfalfa silage, but species corresponding to those in the inoculants displayed variations in pers ... | 2006 | 16205920 |
| a comparative study and phage typing of silage-making lactobacillus bacteriophages. | to investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. the seven phages of groups i, ii and v were active on lactobacillus plantarum and lactobacillus pentosus. phage phipy4 (group iii) infected both l. casei and lactobacillus rhamnosus. phage phipy5 (group iv) specifically infected lactobacillu ... | 2003 | 16233449 |
| lactobacillus arizonensis is a later heterotypic synonym of lactobacillus plantarum. | the 'lactobacillus plantarum group' encompasses the taxa lactobacillus plantarum subsp. plantarum, lactobacillus plantarum subsp. argentoratensis, lactobacillus paraplantarum, lactobacillus pentosus and lactobacillus arizonensis. in this study, the phylogenetic position of l. arizonensis was examined using 16s rrna gene-specific methodologies (16s rrna sequencing and ribotyping) and genomic dna-based investigations [repetitive extragenic palindromic dna (rep)-pcr and dna-dna hybridization]. our ... | 2005 | 16280514 |
| microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods. | fermentation of capers (the fruits of capparis sp.) was studied by molecular and culture-independent methods. a lactic acid fermentation occurred following immersion of caper berries in water, resulting in fast acidification and development of the organoleptic properties typical of this fermented food. a collection of 133 isolates obtained at different times of fermentation was reduced to 75 after randomly amplified polymorphic dna (rapd)-pcr analysis. isolates were identified by pcr or 16s rrna ... | 2005 | 16332762 |
| incompatibility of lactobacillus vectors with replicons derived from small cryptic lactobacillus plasmids and segregational instability of the introduced vectors. | three new lactobacillus vectors based on cryptic lactobacillus plasmids were constructed. the shuttle vector plp3537 consists of a 2.3-kb plasmid from lactobacillus pentosus md353, an erythromycin resistance gene from staphylococcus aureus plasmid pe194, and puc19 as a replicon for escherichia coli. the vectors plpe317 and plpe323, which do not contain e. coli sequences, were generated by introducing the erythromycin resistance gene of pe194 into a 1.7- and a 2.3-kb plasmid from l. pentosus md35 ... | 1991 | 16348515 |
| recognition site for the side chain of 2-ketoacid substrate in d-lactate dehydrogenase. | replacement of tyr52 with val or ala in lactobacillus pentosus d-lactate dehydrogenase induced high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate. on the other hand, replacements with arg, thr or asp severely reduced the enzyme activity, and the tyr52arg enzyme, the only one that exhibited significant enzyme activity, showed a similar substrate preference to the tyr52val and tyr52ala enzymes. replacement of phe299 with gly or ser greatly reduced the enzyme activity w ... | 2005 | 16428303 |
| molecular characterization of lactic acid bacteria from sourdough breads produced in sardinia (italy) and multivariate statistical analyses of results. | the objective of this work was to investigate the structure and diversity of lactic acid bacteria (lab) communities in sourdough used for the production of traditional breads (carasau, moddizzosu, spianata, zichi) in sardinia. 16s rdna sequencing and randomly amplified polymorphic dna (rapd-pcr) was applied for the identification and typing of the lab isolated from 25 samples of sourdoughs. multivariate statistical techniques were applied to rapd-pcr pattern to study the biological diversity of ... | 2006 | 16464695 |
| the nutritional requirements of lactobacillus pentosus 124-2. | | 1948 | 16561507 |
| metabolism of biotin and oxybiotin by lactobacillus pentosus 124-2. | | 1948 | 16561508 |
| the use of lactobacillus pentosus 1mo to shorten the debittering process time of black table olives (cv. itrana and leccino): a pilot-scale application. | fifty lactobacilli isolated from black table olive brines were evaluated for their salt tolerance, resistance to oleuropein and verbascoside, and ability to grow in modified filter-sterilized brines. a strain of lactobacillus pentosus was selected and used as a starter to ferment, in pilot plant, black olives (itrana and leccino cv.) in brines modified for ph, carbohydrate, and growth factor concentrations, at 28 degrees c. the temperature-controlled fermentation of leccino cv. olives resulted i ... | 2006 | 16719508 |
| changes in volatile compounds and related biochemical profile during controlled fermentation of cv. conservolea green olives. | the effect of controlled fermentation processes on the profile of volatile and other biochemical compounds of cv. conservolea green olives processed by the spanish method was studied. the different treatments included: (a) inoculation with a commercial starter culture of lactobacillus pentosus, (b) inoculation with a wild strain of lactobacillus plantarum isolated from a previous fermentation, (c) uninoculated spontaneous process (control). microbial growth, ph, titratable acidity, reducing suga ... | 2006 | 16943076 |
| the use of a d-optimal design to model the effects of temperature, nacl, type and acid concentration on lactobacillus pentosus iglac01. | to study the effects of temperature, nacl and acid (hcl, citric, acetic and lactic) concentrations on the specific growth rate (mu), lag phase (lambda), and h0 of lactobacillus pentosus iglac01. | 2006 | 16968303 |
| tartaric acid recovery from distilled lees and use of the residual solid as an economic nutrient for lactobacillus. | the recovery of tartaric acid (ta) from distilled vinification lees coming from the white and red winemaking technology was optimized using response surface methodology and statistica 5.0 software. the sequential treatment of dissolving ta and further calcium tartrate (cat) precipitation could be used to recover up to 92.4% of the initial ta when distilled white lees were used. the residual lees were employed as economic nutrients for lactic acid production by lactobacillus pentosus cect-4023 us ... | 2006 | 17002469 |
| lactic acid fermentation stimulated iron absorption by caco-2 cells is associated with increased soluble iron content in carrot juice. | an in vitro digestion/caco-2 cell model was applied to explore the impact of lactic acid (la) fermentation by lactobacillus pentosus fsc1 and leuconostoc mesenteroides fsc2 on the fe bioavailability of carrot juice. the redox state of fe in fermented carrot juice was also assessed as a crucial factor for absorption. la fermentation was shown to improve mineral solubility to different extents at simulated physiological conditions: mn (2-fold); fe (1.5-1.7-fold); zn (1.2-fold); cu (1-fold). solubl ... | 2006 | 17010230 |
| culture conditions determine the balance between two different exopolysaccharides produced by lactobacillus pentosus lps26. | lactobacillus pentosus lps26, isolated from a natural fermentation of green olives, produces a capsular polymer constituted of two exopolysaccharides (eps): eps a, a high-molecular-weight (high-mw) polysaccharide (1.9x10(6) da) composed of glucose and rhamnose (3:1), and eps b, a low-mw polysaccharide (3.3x10(4) da) composed of glucose and mannose (3:1). fermentation experiments in a chemically semidefined medium with different carbon sources (glucose, fructose, mannitol, and lactose) showed tha ... | 2006 | 17012595 |
| treatment of green table olive solutions with ozone. effect on their polyphenol content and on lactobacillus pentosus and saccharomyces cerevisiae growth. | disposition of green table olives alkaline solutions (gtos) remains a major task for the industry with serious environmental implications. the presence of high concentrations of polyphenols in olives also presents a problem for direct brining and successful fermentation step. in this study we evaluated the effect of ozone treated gtos on the growth of saccharomyces cerevisiae and lactobacillus pentosus. gtos were treated with ozone for 24 and 72 h. the treatment caused a rapid destruction of pol ... | 2007 | 17174427 |
| enzymatic activities of lactic acid bacteria isolated from cornetto di matera sourdoughs. | forty-one strains of lactic acid bacteria (lab) isolated from cornetto di matera sourdoughs were screened for their enzymatic activities, to elucidate their possible roles during the fermentation process. urease, peptidase, phytase, phosphatase and beta-glucosidase activities were measured spectrophotometrically using synthetic substrates. proteolytic activities were examined in model doughs, using neutral and acidified sterile doughs as controls. all strains had low urease, glutamyl aminopeptid ... | 2007 | 17174429 |
| characterisation and biochemical properties of predominant lactic acid bacteria from fermenting cassava for selection as starter cultures. | a total of 375 lactic acid bacteria were isolated from fermenting cassava in south africa, benin, kenya and germany, and were characterised by phenotypic and genotypic tests. these could be divided into five main groups comprising strains of facultatively heterofermentative rods, obligately heterofermentative rods, heterofermentative cocci, homofermentative cocci and obligately homofermentative rods, in decreasing order of predominance. most of the facultatively heterofermentative rods were iden ... | 2007 | 17188771 |
| characterization of lactobacilli isolated from caper berry fermentations. | to determine the metabolic and functional properties of lactobacilli isolated from caper fermentation. | 2007 | 17241365 |