| transformation of n epsilon-cbz-l-lysine to cbz-l-oxylysine using l-amino acid oxidase from providencia alcalifaciens and l-2-hydroxy-isocaproate dehydrogenase from lactobacillus confusus. | biotransformations were developed to oxidize n epsilon-carbobenzoxy(cbz)-l-lysine and to reduce the product keto acid to l-cbz-oxylysine. lysyl oxidase (l-lysine: o2 oxidoreductase, ec 1.4.3.14) from trichoderma viride was relatively specific for l-lysine and had very low activity with n epsilon-substituted derivatives. l-amino acid oxidase (l-amino acid: o2 oxidoreductase [deaminating], ec 1.4.3.2) from crotalus adamanteus venom had low activity with l-lysine but high activity with n epsilon-fo ... | 1992 | 1368913 |
| nucleotide sequence of 16s ribosomal rna from lactobacillus viridescens and lactobacillus confusus. | | 1990 | 1694026 |
| recovery of vancomycin-resistant gram-positive cocci from pediatric liver transplant recipients. | between november 1988 and october 1989, 49 first-time pediatric liver transplant recipients at the children's hospital of pittsburgh were prospectively monitored for the presence of stool colonization and the development of disease caused by vancomycin-resistant gram-positive cocci (vrgpc). quantitative stool culturing was done on a weekly basis, and cultures were planted onto a selective medium for vrgpc. isolates for which the mic was greater than or equal to 8 were considered resistant to van ... | 1991 | 1774255 |
| abscess caused by vancomycin-resistant lactobacillus confusus. | several isolates of vancomycin-resistant lactobacillus confusus from human sources have been described, but to our knowledge, no well-documented infection attributable to this organism has been published. a thumb abscess caused by this bacterium in a healthy 49-year-old male is reported here. he was successfully treated by surgical drainage and cephalothin. | 1991 | 1774335 |
| recovery of vancomycin-resistant gram-positive cocci from children. | a cross-sectional survey of vancomycin-resistant gram-positive cocci (vrgpc) in the feces of children was initiated after several bacteremic infections with these organisms occurred at our hospital. a selective medium consisting of colistin-nalidixic acid agar, 5% sheep blood, vancomycin (5 mg/liter), and amphotericin b (8 mg/liter) was developed to isolate vrgpc. a single stool specimen submitted to the clinical microbiology laboratory from each of 48 patients was inoculated onto the medium. pl ... | 1990 | 2108993 |
| clinical and microbiologic characteristics of pediococci. | over a 43-month period, 23 separate isolates of nonenterococcal alpha- and nonhemolytic streptococci were reported by our clinical microbiology laboratory to be resistant to vancomycin. this constituted 0.32% of nonenterococcal alpha- and nonhemolytic streptococci reported and 4.4% of such streptococci upon which susceptibility testing was performed. of 13 isolates which were available for further study, all were highly resistant to vancomycin (mic greater than or equal to 1,024 micrograms/ml), ... | 1990 | 2380362 |
| cloning, sequencing and expression of the l-2-hydroxyisocaproate dehydrogenase-encoding gene of lactobacillus confusus in escherichia coli. | the gene (l-hicdh) encoding l-2-hydroxyisocaproate dehydrogenase (l-hicdh) from lactobacillus confusus was cloned in escherichia coli. a 69-mer oligodeoxyribonucleotide probe, derived to be complementary to the n-terminal amino acid (aa) coding sequence, was used for screening. the complete nucleotide (nt) sequence of the l-hicdh gene was determined. the 5'-end of the mrna was mapped by primer extension and the promoter identified. downstream from the l-hicdh gene is a typical rho-independent te ... | 1989 | 2684788 |
| identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria. | a total of 84 of 150 vancomycin-resistant (defined as no inhibition of bacterial growth around a 30-micrograms vancomycin disk placed on 5% sheep blood-trypticase soy agar [bbl microbiology systems, cockeysville, md.]) bacteria were definitively identified by determining the phenotypic criteria. the identity of representatives was also confirmed by dna-dna hybridizations. the following strains were identified: 1 enterococcus faecium, 18 leuconostoc mesenteroides, 15 leuconostoc citreum, 9 leucon ... | 1989 | 2723037 |
| some slime-forming heterofermentative species of the genus lactobacillus. | lactobacillus coprophilus subsp. confusus (ncdo 1586) and 18 other strains of slime-forming heterofermentative lactobacilli obtained from diverse sources are considered to form a new species on the basis of their physiological characteristics and similarities of their pyruvate reductases and lactate dehydrogenases. it is suggested that these strains should be named lactobacillus confusus (holzapfel & kandler) comb. nov. (l. coprophilus subsp. confusus holzapfel & kandler), reasons being given. t ... | 1972 | 4259626 |
| [taxonomy of the species lactobacillus beijerinck. vi. lactobacillus coprophilus subsp. confusus nov. subsp., a new variety of the subspecies betabacterium]. | | 1969 | 4913652 |
| crystal structure of l-2-hydroxyisocaproate dehydrogenase from lactobacillus confusus at 2.2 a resolution. an example of strong asymmetry between subunits. | l-2-hydroxyisocaproate dehydrogenase (l-hicdh) from lactobacillus confusus, a homotetramer with a molecular mass of 33 kda per subunit, belongs to the protein family of the nad(+)-dependent l-2-hydroxycarboxylate dehydrogenases. l-hicdh was crystallized with ammonium sulphate as precipitant in the presence of nad+. the crystals belong to the trigonal space group p3(2)21, with a = 135.9 a and c = 205.9 a, and diffract x-rays to 2.2 a resolution. the crystal structure was solved by patterson searc ... | 1995 | 7643402 |
| phylogenetic analysis of some leuconostocs and related organisms as determined from large-subunit rrna gene sequences: assessment of congruence of small- and large-subunit rrna derived trees. | the large-subunit ribosomal rna gene sequences of leuconostoc carnosum, leuc. mesenteroides, leuc. oenos, leuc. paramesenteroides and lactobacillus confusus were determined by direct sequencing of enzymatically-amplified dna. comparative analysis of the large-subunit rdna sequences revealed that the leuconostocs form three distinct lines of descent: leuconostoc sensu stricto, leuc. paramesenteroides group of organisms, and leuc. oenos. the distance matrix tree constructed from the large-subunit ... | 1993 | 7683639 |
| deletion variants of l-hydroxyisocaproate dehydrogenase. probing substrate specificity. | the substrate specificity and catalytic activity of the dinucleotide-dependent l-2-hydroxyisocaproate dehydrogenase from lactobacillus confusus (l-hicdh) have been altered by modifying an enzyme region which is assumed to be involved in substrate recognition. the design of the variant enzymes was based on an amino acid alignment of the modified region with the functionally related l-lactate dehydrogenases. the best absolute sequence similarity for a protein with known tertiary structure was foun ... | 1994 | 8055963 |
| lactobacillus- and bifidobacterium-mediated antigenotoxicity in the colon of rats. | lactic acid bacteria (lab) are proposed to have several beneficial effects, including the inactivation of carcinogens. we have studied the potential of lactobacillus acidophilus (from a commercially available yogurt), lactobacillus gasseri (p79), lactobacillus confusus (dsm20196), streptococcus thermophilus (ncim 50083), bifidobacterium breve and bifidobacterium longum (from human infant stool) to prevent the induction of dna damage by n-methyl-n'-nitro-n-nitrosoguanidine (mnng, 7.5 mg/kg body w ... | 1996 | 8910918 |
| munkoyo beverage, a traditional zambian fermented maize gruel using rhynchosia root as amylase source. | a typical munkoyo beverage was made by fermenting rhynchosia heterophylla root extract-cooked maize meal mixture with lactobacillus confusus lzi and saccharomyces cerevisiae yz20. the fermented munkoyo beverage had a ph of 3.3, lactic acid content of 60 mmol/l, ethanol 320-410 mmol/l and a characteristic 'munkoyo' aroma. l. confusus, used alone, produced a beverage with a faint munkoyo flavour note whilst beverage produced with s. cerevisiae alone seemed not to have a typical munkoyo note. r. he ... | 1997 | 9039570 |
| modified substrate specificity of l-hydroxyisocaproate dehydrogenase derived from structure-based protein engineering. | l-2-hydroxyisocaproate dehydrogenase (l-hicdh) is characterized by a broad substrate specificity and utilizes a wide range of 2-oxo acids branched at the c4 atom. modifications have been made to the sequence of the nad(h)-dependent l-hicdh from lactobacillus confusus in order to define and alter the region of substrate specificity towards various 2-oxocarbonic acids. all variations were based on a 3d-structure model of the enzyme using the x-ray coordinates of the functionally related l-lactate ... | 1997 | 9153075 |
| identification of lactic acid bacteria from chili bo, a malaysian food ingredient. | ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ... | 1999 | 9925588 |
| phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species triticum durum and triticum aestivum) sourdoughs of southern italy. | the microflora of 25 wheat sourdoughs from the apulia region, southern italy, was characterized. the sourdoughs were mainly produced from triticum durum wheat. the number of lactic acid bacteria and yeasts ranged from ca. log 7.5 to log 9.3 colony forming units (cfu)/g and from log 5.5 to log 8.4 cfu/g, respectively. about 38% of the 317 isolates of lactic acid bacteria were identified by conventional physiological and biochemical tests. phenotypic identification was confirmed by 16s rdna and 16 ... | 2001 | 11252516 |
| weissella confusa (basonym: lactobacillus confusus) bacteremia: a case report. | infection with lactobacillus is rare, and only a handful of species have been identified as being clinically significant: lactobacillus casei, lactobacillus rhamnosus, and lactobacillus leichmannii. the literature contains one case report of bacteremia caused by weissella confusa (basonym: lactobacillus confusus), but the clinical significance of the infection was unclear. we describe a case of w. confusa bacteremia in a 46-year-old man with a history of abdominal aortic dissection and repair. t ... | 2001 | 11283096 |
| taxonomic study of weissella confusa and description of weissella cibaria sp. nov., detected in food and clinical samples. | a taxonomic study was conducted to clarify the relationships of two bacterial populations belonging to the genus weissella. a total of 39 strains originating mainly from malaysian foods (22 strains) and clinical samples from humans (9 strains) and animals (6 strains) were analysed using a polyphasic taxonomic approach. the methods included classical phenotyping, whole-cell protein electrophoresis, 16s and 23s rdna rflp (ribotyping), determination of 16s rdna sequence homologies and dna-dna reass ... | 2002 | 11837296 |
| weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. | a gram-positive, catalase-negative, non-sporulating, facultatively anaerobic, short rod-shaped bacterium, with cells measuring 0.3-0.5 x 1-2 microm and designated strain chj3t, was isolated from partially fermented kimchi, a traditional korean fermented vegetable food. the strain produced co2 gas, d-lactate from glucose and dextran from sucrose and hydrolysed aesculin and arginine. it also fermented n-acetylglucosamine, amygdalin, arbutin, cellobiose, d-fructose, galactose, beta-gentiobiose, glu ... | 2002 | 11931163 |
| isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barley cultivars. | malt has a complex microbial population, which changes as the malting process commences. little is known about the proliferation of lactic acid bacteria (lab) in each of the malting phases. in this study, we determined the number of lab present in the different phases of malting with clipper and prisma barley cultivars. the strains were identified to species level by using numerical analysis of total soluble cell protein patterns, rapd-pcr banding patterns and 16s rrna sequencing. the number of ... | 2002 | 12038579 |
| weissella soli sp. nov., a lactic acid bacterium isolated from soil. | phylogenetic analysis of the 16s rrna gene of bacterial isolates from garden soil showed relatedness to weissella kandleri and weissella confusa. however, the sequences had notable differences, and dna-dna hybridizations confirmed that the isolates are separate from these two species. the isolates could be further distinguished from all previously described weissella species by electrophoretic analysis of whole-cell proteins, as well as by the results from different biochemical tests. the name w ... | 2002 | 12054246 |
| effect of weissella confusa strain pl9001 on the adherence and growth of helicobacter pylori. | viable and nonviable weissella confusa strain pl9001 inhibited the binding of helicobacter pylori to human gastric-cell line mkn-45 cells by more than 90%. spent culture supernatant of pl9001 rapidly decreased the viability of h. pylori, rupturing cell walls. the results suggest that pl9001 is a probiotic that can reduce the infectivity and persistence of h. pylori. | 2002 | 12200324 |
| a pcr-based method for identification of lactobacilli at the genus level. | we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ... | 2002 | 12351242 |
| microbiological and fermentation characteristics of togwa, a tanzanian fermented food. | selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ... | 2003 | 12423921 |
| isolation, characterisation and identification of lactic acid bacteria from bushera: a ugandan traditional fermented beverage. | one hundred and thirteen strains of lactic acid bacteria (lab) were selected from 351 isolates from 15 samples of traditionally fermented household bushera from uganda and also from laboratory-prepared bushera. isolates were phenotypically characterised by their ability to ferment 49 carbohydrates using api 50 chl kits and additional biochemical tests. coliforms, yeasts and lab were enumerated in bushera. the ph, volatile organic compounds and organic acids were also determined. the lab counts i ... | 2003 | 12494920 |
| fatal case of endocarditis due to weissella confusa. | this is the first reported case of endocarditis due to the lactobacillus-like vancomycin-resistant gram-positive bacillus weissella confusa. full identification and susceptibility testing of lactobacillus-like organisms recovered in blood culture should be performed for patients with clinical presentations that suggest endocarditis. | 2003 | 12734290 |
| novel leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product. | to determine the dominant microorganisms involved in kimchi fermentation and to examine their effect on kimchi fermentation, we randomly isolated and characterized 120 lactic acid bacteria from kimchi during a 5-day fermentation at 15 degrees c. leuconostoc citreum was dominant during the early and mid-phases of kimchi fermentation whereas lactobacillus sake/lactobacillus curvatus or lactobacillus brevis were found during later stages. eighty-two out of 120 isolates (68%) were identified as leuc ... | 2003 | 14574101 |
| weissella confusa infection in primate (cercopithecus mona). | | 2003 | 14626220 |
| characterization and identification of lactic acid bacteria in "morcilla de burgos". | a total of 176 lactic acid bacteria (lab) isolated from a typical spanish blood sausage called "morcilla de burgos" were identified by means of phenotypic characteristics and 16s rdna rflp (ribotyping). lab were isolated from "morcilla" of different producers and in different storage periods, which includes unpackaged, vacuum and modified atmosphere packaged "morcilla" and vacuum packed and pasteurised "morcilla". the knowledge of specific spoilage bacteria of "morcilla de burgos" will be useful ... | 2005 | 15582739 |
| analysis of kimchi microflora using denaturing gradient gel electrophoresis. | a polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) technique was used to determine the microfloral composition during the fermentation of kimchi, a traditional korean fermented vegetable food. the kimchi was fermented at 10 degrees c or 20 degrees c for 30 or 20 days, respectively. dgge of the partially amplified 16s rdna was performed and the most intense bands sequenced. the application of this culture-independent molecular technique determined that the lactic acid ... | 2005 | 15992614 |
| potential application of ribosomal intergenic spacer analysis to the microbial community analysis of agronomic products. | ribosomal intergenic spacer analysis (risa) has been applied to the microbial community analysis of agronomic products in combination with a simple and rapid dna extraction method, consisting of a one-step extraction and two-step purification, for a variety of agronomic products. risa appears to be a useful tool for the study of the community structures of food-associated microbes and their use as a unique fingerprinting signature for each agronomic product. sequencing analyses of amplicons gene ... | 2005 | 15998122 |
| characterisation of the microflora of attiéké, a fermented cassava product, during traditional small-scale preparation. | attiéké is a fermented cassava product consumed mainly in cote d'ivoire. the aim of this study was to characterise the attiéké fermentation by examining products from 15 small-scale production sites at various stages of its preparation. for the preparation of attiéké, fresh cassava is grated to a pulp and inoculated with 10% of a spontaneous traditional inoculum. the inocula contained aerobic mesophiles at mean numbers of 8.2 x 10(7) cfu/g and lactic and acetic acids at mean concentrations of 0. ... | 2006 | 16213052 |
| phenotypic identification and technological properties of lactic acid bacteria isolated from traditionally processed fish products of the eastern himalayas. | sukako maacha, gnuchi, sidra and sukuti are traditional smoked and sun-dried fish products of the eastern himalayan regions of nepal and india. a total of 40 samples of sukako maacha (14), gnuchi (6), sidra (10) and sukuti (10) were collected and were analysed for microbial load. population of lactic acid bacteria (lab) as well as aerobic mesophilic counts ranged from 4.7-8.3 to 5.1-8.5 log cfu g(-1), respectively. a total of 189 strains of lab were isolated from sukako maacha, gnuchi, sidra and ... | 2006 | 16278028 |
| isolation and characterization of microorganisms associated with the traditional sorghum fermentation for production of sudanese kisra. | sorghum flour obtained from sudan was mixed with water in a 1:2 (wt/vol) ratio and fermented at 30 degrees c for 24 h. the bacterial populations increased with fermentation time and reached a plateau at approximately 18 h. at the end of 24 h, sorghum batter ph had dropped from 5.95 to 3.95 and the batter had a lactic acid content of 0.80%. the microbial population during the 24 h of fermentation consisted of bacteria (pediococcus pentosaceus, lactobacillus confusus, lactobacillus brevis, lactoba ... | 1991 | 16348551 |
| lactic acid bacteria isolated from canine faeces. | lactic acid bacteria (lab) were isolated and sequenced from the faeces of healthy dogs. five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine. | 2006 | 16834600 |
| defined multi-species semi-liquid ready-to-use sourdough starter. | this paper aimed at setting up a protocol for the manufacture of a defined multi-species semi-liquid ready-to-use sourdough starter consisting of lactic acid bacteria (lab) selected on the basis of their capacity of rapid acidification and adaptation to several technological factors. preliminarily, 56 strains of sourdough lab were screened based on the acidification kinetics during sourdough fermentation. the influence of temperature (25-37 degrees c), nacl (2%, w/w, of wheat flour), initial cel ... | 2007 | 16943090 |
| alteration of the canine small-intestinal lactic acid bacterium microbiota by feeding of potential probiotics. | five potentially probiotic canine fecal lactic acid bacterium (lab) strains, lactobacillus fermentum lab8, lactobacillus salivarius lab9, weissella confusa lab10, lactobacillus rhamnosus lab11, and lactobacillus mucosae lab12, were fed to five permanently fistulated beagles for 7 days. the survival of the strains and their potential effects on the indigenous intestinal lab microbiota were monitored for 17 days. denaturing gradient gel electrophoresis (dgge) demonstrated that the five fed lab str ... | 2006 | 17021203 |
| antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms. | non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ... | 2007 | 17049799 |
| severe infective endocarditis of native valves caused by weissella confusa detected incidentally on echocardiography. | weissella confusa is gram-positive, catalase-negative, short rod or coccobacillus that has been isolated from a wide variety of habitats. we describe a rare, severe infective endocarditis of native valves caused by w. confusa that was detected incidentally on echocardiography. the patient recovered completely with valvular replacement plus antibiotic therapy. | 2007 | 17052757 |
| an irreversible and kinetically controlled process: thermal induced denaturation of l-2-hydroxyisocaproate dehydrogenase from lactobacillus confusus. | the thermal denaturation of lactobacillus confusus l-2-hydroxyisocaproate dehydrogenase (l-hicdh) has been studied by differential scanning calorimetry (dsc). the stability of this enzyme has been investigated at different ph conditions. the results of this study indicate that the thermal denaturation of this enzyme is irreversible and the t(m) is dependent on the scan-rate, which suggests that the denaturation process of l-hicdh is kinetically determined. the heat capacity function of l-hicdh s ... | 2007 | 17205397 |
| characterization of italian cheeses ripened under nonconventional conditions. | four italian cheeses (casciotta di urbino, barricato san martino, vento d'estate, and ubriaco di raboso) nonconventionally ripened under different plant materials (walnut leaves, herbs, hay, and wine by-products, respectively) were compared for compositional, microbiological, biochemical, and volatile profile characteristics. mean values for gross composition were rather similar. because primary starters were not used for manufacture, the endogenous lactic acid bacteria were mainly present (7.0 ... | 2007 | 17517708 |
| lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin. | to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development. | 2007 | 17650194 |
| molecular profiling of lactobacillus, streptococcus, and bifidobacterium species in feces of active racehorses. | diversity and compositions of the lactobacillus, streptococcus, and bifidobacterium group in the feces of six healthy, actively racing horses (thoroughbreds) were analyzed by using pcr-denaturing gradient gel electrophoresis (dgge) and real-time pcr with primer sets specific for each group. pcr-dgge analysis of the feces showed that lactobacillus equi, lactobacillus johnsonii, a phylogenetic relative of lactobacillus salivarius, a phylogenetic relative of lactobacillus gastricus, and weissella c ... | 2007 | 17726300 |
| conversion of isoflavone glucosides to aglycones in soymilk by fermentation with lactic acid bacteria. | four lactic acid bacteria (lab), lactobacillus paraplantarum km, enterococcus durans kh, streptococcus salivarius hm and weissella confusa jy, were isolated from humans and tested for their capabilities of converting isoflavone glucosides to aglycones in soymilk. changes in growth, ph, and titratable acidity (ta) were investigated during fermentation at 37 degrees c for 12 h. after 6 to 9 h of fermentation, each population of 4 lab reached 10(8) to 10(9) cfu/ml. the initial ph of 6.3 +/- 0.1 dec ... | 2007 | 17995840 |
| a differential medium for lactic acid-producing bacteria in a mixed culture. | modified deman-rogosa sharpe agar containing bromophenol blue (mmrs-bpb) was tested as a medium for counting and differentiation of each lactic acid-producing bacterium (lab), especially in a mixed culture. | 2008 | 18444977 |
| nmr spectroscopic analysis of exopolysaccharides produced by leuconostoc citreum and weissella confusa. | dextrans are the main exopolysaccharides produced by leuconostoc species. other dextran-producing lactic acid bacteria include streptococci, lactobacilli, and weissella species. commercial production and structural analysis has focused mainly on dextrans from leuconostoc species, particularly on leuconostoc mesenteroides strains. in this study, we used nmr spectroscopy techniques to analyze the structures of dextrans produced by leuconostoc citreum e497 and weissella confusa e392. the dextrans w ... | 2008 | 18452899 |
| use of lactobacillus strains to start cassava fermentations for gari production. | two lactobacillus strains, lactobacillus plantarum bfe 6710 and lactobacillus fermentum bfe 6620, were used to start cassava fermentations in a pilot study under field production conditions in kenya, to determine their potential to establish themselves as predominant lactobacilli during the fermentation. predominant strains from three fermentations were isolated throughout the 48 h fermentation period. the use of these strains in high numbers clearly resulted in 1 to 2 log higher lactic acid bac ... | 2008 | 18834642 |
| microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge. | the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ... | 2008 | 18838186 |
| taxonomic structure and monitoring of the dominant population of lactic acid bacteria during wheat flour sourdough type i propagation using lactobacillus sanfranciscensis starters. | the structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type i propagation by using singly nine strains of lactobacillus sanfranciscensis. under back-slopping propagation with wheat flour type 0 f114, cell numbers of presumptive lactic acid bacteria varied slightly between and within starters. as determined by randomly amplified polymorphic dna-pcr and restriction endonuclease analysis-pulsed-field gel electrophoresis analyses, only ... | 2009 | 19088320 |
| use of autochthonous starters to ferment red and yellow peppers (capsicum annum l.) to be stored at room temperature. | strains of lactobacillus curvatus, leuconostoc mesenteroides, lactobacillus plantarum and weissella confusa were identified from raw red and yellow peppers (ryps) by partial 16s rrna gene sequence and subjected to typing by random amplified polymorphic dna-polymerase chain reaction (rapd-pcr) analysis. l. plantarum pe21, l. curvatus pe4 and w. confusa pe36 were selected based on the kinetics of growth and acidification, and used as the autochthonous mixed starter for the fermentation of ryps. a ... | 2009 | 19217182 |
| antifungal activity of strains of lactic acid bacteria isolated from a semolina ecosystem against penicillium roqueforti, aspergillus niger and endomyces fibuliger contaminating bakery products. | thirty samples of italian durum wheat semolina and whole durum wheat semolina, generally used for the production of southern italy's traditional breads, were subjected to microbiological analysis in order to explore their lactic acid bacteria (lab) diversity and to find strains with antifungal activity. a total of 125 presumptive lab isolates (gram-positive and catalase-negative) were characterized by repetitive extragenic palindromic-pcr (rep-pcr) and sequence analysis of the 16s rrna gene, lea ... | 2009 | 19243908 |
| the microbiota of lafun, an african traditional cassava food product. | lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ... | 2009 | 19493582 |
| polyphasic taxonomic studies of lactic acid bacteria associated with tunisian fermented meat based on the heterogeneity of the 16s-23s rrna gene intergenic spacer region. | the objective of this work was to investigate the structure and diversity of lactic acid bacteria (lab) communities in traditionally fermented meat collected from different areas of tunisia. a polyphasic study, which involves phenotypic tests and ribosomal dna-based techniques, was used to identify gram-positive and catalase-negative isolates. pcr amplification of the 16s-23s rdna isr of 102 isolates and other reference lab strains gave (1) one type of rrn operon (m-isr) for lactococci, (2) two ... | 2009 | 19669730 |
| lactobacillus and bifidobacterium diversity in horse feces, revealed by pcr-dgge. | lactobacillus equi, lactobacillus hayakitensis, lactobacillus johnsonii, and weissella confusa/cibaria were the dominant species in 12 south african horses. the bifidobacterium-group was detected in the feces of only one of the 12 horses. sequencing of the nested-pcr amplicon identified the bifidobacterium-group as parascardovia denticolens. cell numbers of l. equi, l. hayakitensis, and w. confusa/cibaria were consistent in all samples. p. denticolens, bifidodobacterium pseudolongum, and a phylo ... | 2009 | 19730939 |
| in situ production and analysis of weissella confusa dextran in wheat sourdough. | several lactic acid bacteria belonging to the genera leuconostoc, lactobacillus, and weissella have been introduced to wheat sourdough baking for in situ production of exopolysaccharides. this is considered a novel method for improving the shelf-life, volume and nutritional value of bread without additives. however, in situ production of exopolysaccharides during sourdough fermentation is challenged by simultaneous acidification due to metabolic activities of the bacteria, which may significantl ... | 2009 | 19747607 |
| screening of lactic acid bacteria from indonesia reveals glucansucrase and fructansucrase genes in two different weissella confusa strains from soya. | homopolysaccharide (glucan and fructan) synthesis from sucrose by sucrase enzymes in lactic acid bacteria (lab) has been well studied in the genera leuconostoc, streptococcus and lactobacillus. this study aimed to identify and characterize genes encoding glucansucrase/glucosyltransferase (gtf) and fructansucrases/fructosyltransferase (ftf) enzymes from genomic dna of 'rare' indonesian exopolysaccharide-producing lab. from a total of 63 exopolysaccharide-producing lab isolates obtained from foods ... | 2009 | 19758326 |
| genotypic diversity of lactic acid bacteria isolated from african traditional alkaline-fermented foods. | to identify and compare lactic acid bacteria (lab) isolated from alkaline fermentations of cassava (manihot esculenta crantz) leaves, roselle (hibiscus sabdariffa) and african locust bean (parkia biglobosa) seeds for production of, respectively, ntoba mbodi, bikalga and soumbala. | 2010 | 19895650 |
| solid state fermentation for extracellular polysaccharide production by lactobacillus confusus with coconut water and sugar cane juice as renewable wastes. | extracellular polysaccharide (eps) production by lactobacillus confusus in liquid and solid state fermentation was carried out using coconut water and sugarcane juice as renewable wastes. high concentrations of eps of 62 (sugarcane juice) and 18 g/l of coconut water were produced in solid state fermentation when nitrogen sources were reduced 5-fold from the original medium. | 2010 | 20139601 |
| characterization of dextran-producing weissella strains isolated from sourdoughs and evidence of constitutive dextransucrase expression. | the study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. in this study, several dextran-producing strains, weissella cibaria and weissella confusa, isolated from sourdough, were characterized according to carbohydrate fermentation, repetitive element-pcr fingerprinting using (gtg)(5) primers and glucans ... | 2010 | 20722740 |
| bacteraemia caused by weissella confusa at a university hospital in taiwan, 1997-2007. | clin microbiol infect abstract: human infections caused by weissella confusa are rarely reported. ten patients with bacteraemia caused by w. confusa who were treated at a tertiary-care hospital in taiwan during 1997-2007 were studied. all isolates were initially misidentified as various lactobacillus and leuconostoc species by two commercial automated identification methods, and were confirmed to be w. confusa by 16s rrna sequencing analysis. mics of these isolates for ten antimicrobial agents w ... | 2010 | 21040157 |
| weissella confusa: an unexpected cause of vancomycin-resistant gram-positive bacteremia in immunocompromised hosts. | h. salimnia, g.j. alangaden, r. bharadwaj, t.m. painter, p.h. chandrasekar, m.r. fairfax. weissella confusa: an unexpected cause of vancomycin-resistant gram-positive bacteremia in immunocompromised hosts. transpl infect dis 2011: 13: 294-298. all rights reserved abstract: we report the first case of weissella confusa bacteremia in an allogeneic hematopoietic stem cell transplant patient. after engraftment and discharge, the patient returned with fever and graft failure and was started on an emp ... | 2010 | 21156010 |
| structural analysis of enzyme-resistant isomaltooligosaccharides reveals the elongation of α-(1→3)-linked branches in weissella confusa dextran. | weissella confusa vtt e-90392 is an efficient producer of a dextran that is mainly composed of α-(1→6)-linked d-glucosyl units and very few α-(1→3) branch linkages. a mixture of the chaetomium erraticum endodextranase and the aspergillus niger α-glucosidase was used to hydrolyze w. confusa dextran to glucose and a set of enzyme-resistant isomaltooligosaccharides. two of the oligosaccharides (tetra- and hexasaccharide) were isolated in pure form and their structures elucidated. the tetrasaccharid ... | 2011 | 21207960 |
| analysis of bacterial communities of traditional fermented west african cereal foods using culture independent methods. | in this study, the microbial composition of kunu-zaki and ogi, two popular foods in nigeria produced after natural, uncontrolled fermentation of cereals, was assessed by culture-independent molecular profiling methods. in particular, pcr-denaturing gradient gel electrophoresis and construction of 16s rrna gene clone libraries revealed the presence of diverse bacterial communities. dna sequencing of the highly variable v3 region of the 16s rrna genes obtained from pcr-dgge fingerprints identified ... | 2011 | 21272950 |
| natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16s ribosomal rna and reca gene analysis. | one hundred and fifty-six strains isolated from corn (zea mays l.), forage paddy rice (oryza sativa l.), sorghum (sorghum bicolor l.) and alfalfa (medicago sativa l.) silages prepared on dairy farms were screened, of which 110 isolates were considered to be lactic acid bacteria (lab) according to their gram-positive and catalase-negative characteristics and, mainly, the lactic acid metabolic products. these isolates were divided into eight groups (a-h) based on the following properties: morpholo ... | 2011 | 21282025 |
| novel pcr-based identification of weissella confusa using an aflp-derived marker. | an extensive use of weissella (w.) confusa is currently being made for the production of a variety of fermented foods and beverages although some strains of this species have emerged as opportunistic pathogens for humans and animals. nevertheless, no rapid methods are available for the reliable identification of w. confusa. we developed a novel pcr using aflp (amplified fragment length polymorphism)-derived primers for the rapid and unequivocal identification of w. confusa. fluorescent aflp of 3 ... | 2011 | 21296447 |
| evaluation of the functional potential of weissella and lactobacillus isolates obtained from nigerian traditional fermented foods and cow's intestine. | the characterisation of 24 lactic acid bacteria (lab) isolates from nigerian traditional fermented dairy foods, including some cow's intestine isolates, was conducted in order to select isolates for potential use as probiotics. lab isolates were identified by partial sequencing the 16s rrna gene as belonging to the species lactobacillus paracasei, lactobacillus brevis and mainly weissella confusa. at the end of a characterisation process, 2 l. paracasei and 2 w. confusa isolates were selected, a ... | 2011 | 21482440 |
| weissella confusa bacteremia in a liver transplant patient with hepatic artery thrombosis. | n.p. harlan, r.r. kempker, s.m. parekh, e.m. burd, d.t. kuhar. weissella confusa bacteremia in a liver transplant patient with hepatic artery thrombosis transpl infect dis 2011: 13: 290-293. all rights reserved abstract: a 54-year-old man with a history of nonalcoholic steatohepatitis and hepatocellular carcinoma presented 2 months after an orthotopic liver transplant with fever and abdominal pain. two weeks earlier, he had an hepatic artery thrombosis and a biliary stricture, for which an hepat ... | 2010 | 21504525 |
| weissella confusa: a rare cause of vancomycin resistant gram-positive bacteraemia. | we describe a case of bacteraemia caused by weissella confusa in a 48 year old male who was operated for adenocarcinoma of gastro-esophageal junction and maintained on total parenteral nutrition. blood cultures were positive for vancomycin resistant streptococci like organism which was identified as weissella confusa by 16srdna sequencing. | 2011 | 21596906 |
| 16s rrna-based identification of a glucan-hyperproducing weissella confusa. | a gram-positive, nonmotile, irregular, short, rod-shaped new strain of weissella confusa bacterium was isolated from fermented cabbage. the isolate was physiologically and biochemically characterised. the 16s rdna was amplified by polymerase chain reaction (pcr). the isolate was identified as weissella confusa (genbank accession number: gu138518.1) based on nucleotide homology and phylogenetic analysis. the isolate produces glucansucrase when grown in sucrose-supplemented culture medium which ca ... | 2011 | 21603260 |
| construction of theta-type shuttle vector for leuconostoc and other lactic acid bacteria using pcb42 isolated from kimchi. | the pcb42 plasmid from leuconostoc citreum cb2567, a strain isolated from kimchi, was characterized, and a shuttle vector for escherichia coli and lactic acid bacteria (lab) was constructed. the pcb42 plasmid has a circular structure of 4312bp, a low g+c content, and no single-stranded dna intermediates during replication, which indicates that pcb42 replicates via the theta-type replication mechanism. in silico analysis of this plasmid revealed 6 open reading frames: 1 transposase gene, 1 dna-bi ... | 2012 | 22133745 |
| Probiotic properties of Weissella strains isolated from human faeces. | Three Weissellaconfusa and five Weissellacibaria strains were previously isolated from human faeces and their potential as probiotics was examined in this work. Resistance to low pHs (pH 2.0 and 3.0) and 0.3% bile salt were examined. Enzyme activities, susceptibilities to heat treatment and various antibiotics, and adhesion capacities to Caco-2 cells were also examined. All Weissella strains were killed when exposed to pH 2.0 for 2 h but survived at pH 3.0 with different survival ratios. W. conf ... | 2011 | 22200451 |
| biodiversity of yeasts, lactic acid bacteria and acetic acid bacteria in the fermentation of "shanxi aged vinegar", a traditional chinese vinegar. | shanxi aged vinegar is a famous traditional chinese vinegar made from several kinds of cereal by spontaneous solid-state fermentation techniques. in order to get a comprehensive understanding of culturable microorganism's diversity present in its fermentation, the indigenous microorganisms including 47 yeast isolates, 28 lactic acid bacteria isolates and 58 acetic acid bacteria isolates were recovered in different fermenting time and characterized based on a combination of phenotypic and genotyp ... | 2012 | 22265314 |
| taxonomic and molecular characterization of lactic acid bacteria and yeasts in nunu, a ghanaian fermented milk product. | produced from raw unpasteurized milk, nunu is a spontaneously fermented yoghurt-like product made in ghana and other parts of west africa. despite the importance of nunu in the diet of many africans, there is currently only limited information available on the microorganisms associated with nunu processing. with the aim of obtaining a deeper understanding of the process and as a first step towards developing starter cultures with desired technological properties for nunu production, a microbiolo ... | 2012 | 23541194 |
| antibacterial activities of multi drug resistant myroides odoratimimus bacteria isolated from adult flesh flies (diptera: sarcophagidae) are independent of metallo beta-lactamase gene. | sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for antimicrobial activity against bacteria. antimicrobial studies of myroides spp. are restricted to nosocomial strains. a gram-negative bacterium, myroides sp., was isolated from the gut of adult flesh flies (sarcophaga sp.) and submitted to evaluation of nutritional parameters using biolog gn, 16s rrna gene sequencing, susceptibility to various antimicrobials by disc diffusion method and detection of ... | 2008 | 24031236 |
| effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content. | the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ... | 2013 | 25049913 |
| ethanol production by selected intestinal microorganisms and lactic acid bacteria growing under different nutritional conditions. | to gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (nafld), some intestinal and lactic acid bacteria and one yeast (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, enterococcus fecalis, escherichia coli, lactobacillus acidophilus, lactobacillus fermentum, lactobacillus plantarum, weissella confusa, saccharomyces cerevisiae) were characterized by high performance liquid chromatography for production of ethanol whe ... | 2016 | 26858714 |
| broad-spectrum antifungal-producing lactic acid bacteria and their application in fruit models. | a large-scale screen of some 7,000 presumptive lactic acid bacteria (lab), isolated from animal, human, or plant origin, identified 1,149 isolates with inhibitory activity against the food-spoilage mould penicillium expansum. in excess of 500 lab isolates were subsequently identified to produce a broad spectrum of activity against p. expansum, penicillium digitatum, penicillium notatum, penicillium roqueforti, rhizopus stolonifer, fusarium culmorum, aspergillus fumigatus and rhodotorula mucilagi ... | 2013 | 23160868 |
| monitoring of the microbial communities involved in the soy sauce manufacturing process by pcr-denaturing gradient gel electrophoresis. | soy sauce is a traditional seasoning produced through the fermentation of soybeans and wheat using microbes. in this study, the microbial communities involved in the soy sauce manufacturing process were analyzed by pcr-denaturing gradient gel electrophoresis (pcr-dgge). the bacterial dgge profile indicated that the bacterial microbes in the koji were weissella cibaria (weissella confusa, weissella kimchii, weissella salipiscis, lactobacillus fermentum, lactobacillus plantarum, lactobacillus iner ... | 2012 | 22475947 |
| purification and characterization of bacteriocin produced by weissella confusa a3 of dairy origin. | a dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. therefore, antimicrobial peptides (amps) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. the present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. the bacteriocin was isolated from weissella confusa a3 that was isolated fr ... | 2015 | 26474074 |
| quantification of biofilm exopolysaccharides using an in situ assay with periodic acid-schiff reagent. | a novel approach to the quantification of extracellular polysaccharides in miniaturized biofilms presenting a wide variety of extracellular matrices was developed. the assay used the periodic acid-schiff reagent and was first calibrated on dextran and alginate solutions. then it was implemented on 24-h and 48-h biofilms from three strains known to produce different exopolymeric substances (pseudomonas aeruginosa, bacillus licheniformis, weissella confusa). the assay allowed quantification of the ... | 2016 | 26800864 |
| epicocconone, a sensitive and specific fluorescent dye for in situ quantification of extracellular proteins within bacterial biofilms. | biofilms are ecosystems of closely associated bacteria encapsulated in an extracellular matrix mainly composed of polysaccharides and proteins. a novel approach was developed for in situ quantification of extracellular proteins (epns) in various bacterial biofilms using epicocconone, a natural, fluorescent compound that binds amine residues of proteins. six commercial proteins were tested for their reaction with epicocconone, and bovine serum albumin (bsa) was selected for assay optimization. th ... | 2015 | 25913004 |
| complex microbiota of a chinese "fen" liquor fermentation starter (fen-daqu), revealed by culture-dependent and culture-independent methods. | daqu is a traditional fermentation starter that is used for chinese liquor production. although partly mechanized, its manufacturing process has remained traditional. we investigated the microbial diversity of fen-daqu, a starter for light-flavour liquor, using combined culture-dependent and culture-independent approaches (pcr-dgge). a total of 190 microbial strains, comprising 109 bacteria and 81 yeasts and moulds, were isolated and identified on the basis of the sequences of their 16s rdna (ba ... | 2012 | 22608236 |
| cloning and heterologous expression of the ftfcnc-2(1) gene from weissella confusa mbfcnc-2(1) as an extracellular active fructansucrase in bacillus subtilis. | fructan-exopolysaccharides (fructan-eps) (inulin and levan) and their oligosaccharides (fructooligosaccharides, fos) have drawn considerable interest in the food and pharmaceutical industries. eps-producing lactic acid bacteria have been reported to produce β-fructans (inulin and levan), as well as α-glucans, by the function of sucrase enzymes, i.e., fructansucrase and glucansucrase. a fructansucrase ftfcnc-2(1) gene from weissella confusa strain mbfcnc-2(1) was previously cloned in escherichia ... | 2015 | 25454699 |
| cytotoxicity comparison of quercetin and its metabolites from in vitro fermentation of several gut bacteria. | part of quercetin is coerced into the colon after ingestion and interacts with the gut microbiota. the interaction between quercetin and gut microbiota will influence human health. the cytotoxicity of quercetin and its metabolites from human gut bacteria in vitro fermentation was investigated in this study. the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) (mtt) and agar diffusion disc methods were individually applied in vitro to examine their inhibitory effect on three cultured ... | 2014 | 25015032 |
| isolation and identification of quercetin degrading bacteria from human fecal microbes. | quercetin has a wide range of biological properties. the gut microflora can often modulate its biological activity and their potential health effects. there still is a lack of information about gut bacteria involving in this process. the strains of gut microbes from human feces that can transform quercetin were isolated and identified by in vitro fermentation. the results showed that escherichia coli, stretococcus lutetiensis, lactobacillus acidophilus, weissella confusa, enterococcus gilvus, cl ... | 2014 | 24594786 |
| analysis of bacterial diversity during the fermentation of inyu, a high-temperature fermented soy sauce, using nested pcr-denaturing gradient gel electrophoresis and the plate count method. | the diversity of bacteria associated with the fermentation of inyu, also known as black soy sauce, was studied through the nested pcr-denaturing gradient gel electrophoresis (dgge) of samples collected from the fermentation stages of the inyu production process. the dgge profiles targeted the bacterial 16s rdna and revealed the presence of citrobacter farmeri, enterobacter cloacae, enterobacter hormaechei, enterococcus faecium, klebsiella pneumoniae, pantoea agglomerans, salmonella enterica, ser ... | 2013 | 23200659 |
| characterisation of the spoilage bacterial microbiota in oyster gills during storage at different temperatures. | the spoilage bacterial community in oyster gill was investigated during storage at 4, 10 and 20 °c. aerobic plate counts and ph values were determined. total bacterial dna was extracted from oyster gill and bulk cells of plate count media. the major bacterial species during fresh or different temperatures storage were determined by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). | 2013 | 23696433 |
| microbial shifts associated with necrotic enteritis. | an outbreak of necrotic enteritis (ne) is a complex process requiring one or a number of predisposing factors rather than just the presence of pathogenic clostridium perfringens. examples are dietary influences, such as high levels of non-starch polysaccharides and fishmeal, and factors that evoke epithelial cell damage, such as fusarium mycotoxins in feed and eimeria infections. recent studies have shown that different predisposing factors induce similar shifts in the intestinal microbiota comp ... | 2016 | 26950294 |
| antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens. | the addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives. | 2015 | 26957978 |
| probiotic properties of lactic acid bacteria isolated from human milk. | the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties. | 2016 | 27159339 |
| screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil. | lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ... | 2016 | 26608755 |
| pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage from northwestern argentina. | the diversity of lactic acid bacteria (lab) associated with chicha, a traditional maize-based fermented alcoholic beverage from northwestern argentina, was analyzed using culture-dependent and culture-independent approaches. samples corresponding to 10 production steps were obtained from two local producers at maimará (chicha m) and tumbaya (chicha t). whereas by culture-dependent approach a few number of species (lactobacillus plantarum and weissella viridescens in chicha m, and enterococcus fa ... | 2015 | 25584777 |
| dynamics of microbial community during ensiling direct-cut alfalfa with and without lab inoculant and sugar. | to gain deeper insights into the clostridial community dynamics and chemical transformations during the ensiling of alfalfa. | 2017 | 28370869 |
| molecular cloning of kman coding for mannanase from klebsiella oxytoca kub-cw2-3 and its hybrid mannanase characters. | gene encoding for β-mannanase (e.c 3.2.1.78) from klebsiella oxytoca kub-cw2-3 was cloned and expressed by an e. coli system resulting in 400 times higher mannanase activities than the wild type. a 3314bp dna fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight of 43.2kda. it belonged to the glycosyl hydrolase family 26 (gh26) exhibited low similarity of 50-71% to β-mannanase produced by other microbial so ... | 2016 | 27233126 |
| construction of a shuttle vector based on the small cryptic plasmid pjy33 from weissella cibaria 33. | a cryptic plasmid, pjy33, from weissella cibaria 33 was characterized. pjy33 was 2365 bp in size with a gc content of 41.27% and contained two putative open reading frames (orfs). orf1 encoded a putative hypothetical protein of 134 amino acids. orf2 was 849 bp in size, and its putative translation product exhibited 87% identity with a replication initiation factor from a plasmid from w. cibaria klc140. a weissella-escherichia coli shuttle vector, pjy33e (6.5 kb, em(r)), was constructed by ligati ... | 2015 | 25882072 |
| characterization of a novel dextransucrase from weissella confusa isolated from sourdough. | weissella confusa and weissella cibaria isolated from wheat sourdoughs produce, from sucrose, linear dextrans due to a single soluble dextransucrase. in this study, the first complete gene sequence encoding dextransucrase from a w. confusa strain (lbae c39-2) along with the one from a w. cibaria strain (lbae k39) were reported. corresponding gene cloning was achieved using specific primers designed on the basis of the draft genome sequence of these species. deduced amino acid sequence of w. conf ... | 2013 | 23053097 |
| natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant. | natural lactic acid bacteria (lab) populations in tropical grasses and their fermentation characteristics on silage prepared with cellulase enzyme and lab inoculants were studied. a commercial inoculant lactobacillus plantarum chikuso 1 (ch), a local selected strain lactobacillus casei th14 (th14), and 2 cellulases, acremonium cellulase (ac) and maicelase (mc; meiji seika pharma co. ltd., tokyo, japan), were used as additives to silage preparation with fresh and wilted (6 h) guinea grass and nap ... | 2016 | 27743669 |
| distinctive intestinal lactobacillus communities in 6-month-old infants from rural malawi and southwestern finland. | our aim was to compare the composition and diversity of lactobacillus microbiota in infants living in malawi and southwestern finland. | 2015 | 26049782 |