| transformation of the gram-positive bacterium clavibacter xyli subsp. cynodontis by electroporation with plasmids from the incp incompatibility group. | we report the transformation of a gram-positive bacterium, clavibacter xyli subsp. cynodontis, with several plasmids in the incp incompatibility group from gram-negative bacteria. our results suggest that incp plasmids may be transferable to other gram-positive organisms. after optimizing electroporation parameters, we obtained a maximum of 2 x 10(5) transformants per microgram of dna. the availability of a transformation system for this bacteria will facilitate its use in indirectly expressing ... | 1992 | 1624442 |
| stability of the delta-endotoxin gene from bacillus thuringiensis subsp. kurstaki in a recombinant strain of clavibacter xyli subsp. cynodontis. | deletion of chromosomally inserted gene sequences from clavibacter xyli subsp. cynodontis, a xylem-inhabiting endophyte, was studied in vitro and in planta. we found that nonreplicating plasmid pcg610, which conferred resistance to kanamycin and tetracycline and contained segments of c. xyli subsp. cynodontis genomic dna, integrated into a homologous sequence in the bacterial chromosome. in addition, pcg610 contains two copies of the gene encoding the cryia(c) insecticidal protein of bacillus th ... | 1991 | 1664710 |
| identification and characterization of the ribosomal rna-encoding genes in clavibacter xyli subsp. cynodontis. | clavibacter xyli subsp. cynodontis (cxc) is a xylem-inhabiting bacterial endophyte of bermudagrass. this organism is classified with gram-positive, high g + c content, coryneform-actinomycete bacteria. southern-blot analysis showed that cxc contains only one copy of the ribosomal rna-encoding genes (rrna). a clone containing the rrna genes was isolated from a genomic library of cxc dna cloned in the lambda embl3 vector. the gene cluster was partially sequenced, revealing the gene order 5'-16s-23 ... | 1991 | 1761230 |
| is1237, a repetitive chromosomal element from clavibacter xyli subsp. cynodontis, is related to insertion sequences from gram-negative and gram-positive bacteria. | we describe here a repetitive chromosomal element, which appears to be an insertion sequence, isolated from clavibacter xyli subsp. cynodontis, a gram-positive plant-associated bacterium. the element, is1237, is 905 bp in size, is bounded by 19-bp perfect inverted repeats and 3-bp direct repeats, and appears at least 16 times in the genome. it contains three open reading frames which show similarity to open reading frames from various other insertion sequences. we have found that there are two g ... | 1994 | 7534933 |
| development of a native plasmid as a cloning vector in clavibacter xyli subsp. cynodontis. | a 50-kilobase (kb) cryptic plasmid was found in three geographic isolates of clavibacter xyli subsp. cynodontis (cxc). the dna region essential for replication of the plasmid was cloned in an escherichia coli vector. the resulting vector, which functions as a shuttle vector between e. coli and cxc, was characterized further with respect to its stability and copy number. | 1993 | 7689234 |
| comparison of 16s ribosomal rna genes in clavibacter michiganensis subspecies with other coryneform bacteria. | nearly complete sequences (97-99%) of the 16s rrna genes were determined for type strains of clavibacter michiganensis subsp. michiganensis, clavibacter michiganensis subsp. insidiosus, clavibacter michiganensis subsp. sepedonicus, and clavibacter michiganensis subsp. nebraskensis. the four subspecies had less than 1% dissimilarity in their 16s rrna genes. comparative studies indicated that the c. michiganensis subsp. shared relatively high homology with the 16s rrna gene of clavibacter xyli. fu ... | 1995 | 8590406 |
| isolation of strong promoters from clavibacter xyli subsp. cynodontis using a promoter probe plasmid. | to isolate promoters from clavibacter xyli subsp. cynodontis (c. xyli subsp. cynodontis), we constructed a new promoter probe plasmid and made a c. xyli subsp. cynodontis promoter probe library. two promoters gave over 2500-times stronger expression than the parental plasmid. the promoters were sequenced and compared to other bacterial promoters. these c. xyli subsp. cynodontis promoter regions are gc-rich and do not resemble e. coli promoters, but do resemble a few individual promoters found in ... | 1996 | 8597597 |
| construction of secretion vectors and use of heterologous signal sequences for protein secretion in clavibacter xyli subsp. cynodontis. | we have constructed a vector to assess the ability of heterologous signal sequences to function in the endophytic bacterium clavibacter xyli subsp. cynodontis. this secretion reporter contains the phoa gene from escherichia coli from which the promoter and signal sequences have been deleted. signal sequences of streptomycete origin were cloned into the vector and the level of secreted alkaline phosphatase determined enzymatically and by western blotting. we show that a number of the signal seque ... | 1995 | 8664499 |
| integrative vector for stable transformation and expression of a beta-1,3-glucanase gene in clavibacter xyli subsp. cynodontis. | clavibacter xyli subsp. cynodontis is an endophytic gram-positive bacterium that is able to colonize the xylem tissue of various plants. we constructed an integrative vector that can carry foreign genes into a repetitive chromosomal element of c. xyli subsp. cynodontis. using this vector, we transformed c. xyli subsp. cynodontis with a gene coding for an endoglucanase enzyme under the control of a strong promoter that was previously isolated from the genome of c. xyli subsp. cynodontis. the tran ... | 1998 | 9595657 |
| leifsonia poae gen. nov., sp. nov., isolated from nematode galls on poa annua, and reclassification of 'corynebacterium aquaticum' leifson 1962 as leifsonia aquatica (ex leifson 1962) gen. nov., nom. rev., comb. nov. and clavibacter xyli davis et al. 1984 with two subspecies as leifsonia xyli (davis et al. 1984) gen. nov., comb. nov. | the new genus leifsonia gen. nov. with two new species, leifsonia poae sp. nov. (type strain vkm ac-1401t) and leifsonia aquatica (ex leifson 1962) nom. rev., comb. nov. (the type species, with vkm ac-1400t = dsm 20146t = jcm 1368t as type strain), is proposed to accommodate bacteria found in poa annua root gall, induced by the nematode subanguina radicicola, and 'corynebacterium aquaticum' leifson 1962. further, it is proposed to reclassify clavibacter xyli davis et al. 1984 with two subspecies ... | 2000 | 10826825 |
| transformation and transposon mutagenesis of leifsonia xyli subsp. xyli, causal organism of ratoon stunting disease of sugarcane. | conditions have been developed for genetic transformation and insertional mutagenesis in leifsonia xyli subsp. xyli (lxx), the causal organism of ratoon stunting disease (rsd), one of the most damaging and intractable diseases of sugarcane internationally. transformation frequencies ranged from 1 to 10 colony forming units (cfu)/microg of plasmid dna using clavibacter/escherichia coli shuttle vectors pcg188, pdm302, and pdm306 and ranged from 50 to 500 cfu/microg using cosmid cloning vectors pla ... | 2002 | 11952129 |
| leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate "corynebacterium aquaticum" leifson 1962 and clavibacter xyli subsp. cynodontis davis et al. 1984. | "corynebacterium aquaticum" was first proposed by leifson in 1962 but not included in the approved lists of bacterial names in 1980. this species has been left from reclassification of the genus corynebacterium because of the unusual chemotaxonomic characteristics such as 2,4-diaminobutyric acid (dab) in the peptidoglycan and menaquinones of mk-10 and mk-11. a close relationship of "c. aquaticum" to the genera agromyces and rathayibacter has been pointed out from the viewpoint of chemotaxonomic ... | 1999 | 12501368 |
| establishment of a functional genomics platform for leifsonia xyli subsp. xyli. | leifsonia xyli subsp. xyli, the causal agent of ratoon stunting disease in sugarcane, is a xylem-limited, nutritionally fastidious, slow growing, gram-positive coryneform bacterium. because of the difficulties in growing this bacterium in pure culture, little is known about the molecular mechanisms of pathogenesis. currently, the genome sequence of l. xyli subsp. xyli is being completed by the agronomical and environmental genomes group from the organization for nucleotide sequencing and analysi ... | 2004 | 14964531 |
| fastidious xylem-limited bacterial plant pathogens. | numerous bacteria have been isolated from within plants, and many reported from xylem, but only three species of xylem-limited bacteria (xlb) that are fastidious in cultural requirements, are plant pathogens, and exclusively occupy xylem, have been well characterized. two xlb, xylella fastidiosa and pseudomonas syzygii, are transmitted by sucking insects that feed on xylem sap but are not transmitted mechanically from plant to plant. in contrast, clavibacter xyli is mechanically transmitted to p ... | 1996 | 15012538 |
| characterization of the replicon of a 51-kb native plasmid from the gram-positive bacterium leifsonia xyli subsp. cynodontis. | the 4992-bp replicon of a large cryptic plasmid in the gram-positive bacterium leifsonia xyli subsp. cynodontis was identified and sequenced. the replicon encoded two proteins essential for plasmid replication and stability. the putative replication protein (repa) is homologous to that of the plasmids in mycobacterial plr7 family, while the putative para protein immediately downstream of repa is significantly homologous to the walker-type atpase required for partition of plasmid and chromosome o ... | 2004 | 15212787 |
| the genome sequence of the gram-positive sugarcane pathogen leifsonia xyli subsp. xyli. | the genome sequence of leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. the single circular chromosome of leifsonia xyli subsp. xyli ctcb07 was 2.6 mb in length with a gc content of 68% and 2,044 predicted open reading frames. the analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. many of these pseudogenes, if functional, would likely be involved in the degradation o ... | 2004 | 15305603 |
| bacterial phytopathogens and genome science. | there are now fourteen completed genomes of bacterial phytopathogens, all of which have been generated in the past six years. these genomes come from a phylogenetically diverse set of organisms, and range in size from 870 kb to more than 6mb. the publication of these annotated genomes has significantly helped our understanding of bacterial plant disease. these genomes have also provided important information about bacterial evolution. examples of recently completed genomes include: pseudomonas s ... | 2005 | 16125997 |
| [from the mailing list sin: epidemic of infections caused by 'aquatic' bacteria in patients undergoing hemodialysis via central venous catheters]. | bacteremia due to central venous catheter (cvc) infection is the most frequent complication of cvc use as vascular access for hemodialysis (hd). we report a case of an epidemic of cvc infections caused by 3 strains of unusual bacteria: ralstonia pickettii (rp), leifsonia xyli/leifsonia aquatica (lxa), tsuckamurella strandjordae (ts). from 20/8/01 to 30/9/01, 23 of 35 patients dialyzed via cvcs experienced intra-hd pyrogenic reactions. their hemocultures were positive for: rp (14 pts), lxa (3 pts ... | 2005 | 16267809 |
| integrative cloning, expression, and stability of the cryia(c) gene from bacillus thuringiensis subsp. kurstaki in a recombinant strain of clavibacter xyli subsp. cynodontis. | a bacterial endophyte was engineered for insecticidal activity against the european corn borer. the cryia(c) gene from bacillus thuringiensis subsp. kurstaki was introduced into the chromosome of clavibacter xyli subsp. cynodontis by using an integrative plasmid vector. the integration vectors pcg740 and pcg741 included the replicon pgem5zf(+), which is maintained in escherichia coli but not in c. xyli subsp. cynodontis; tetm as a marker for selection in c. xyli subsp. cynodontis; and a chromoso ... | 1994 | 16349179 |
| signature proteins that are distinctive characteristics of actinobacteria and their subgroups. | the actinobacteria constitute one of the main phyla of bacteria. presently, no morphological and very few molecular characteristics are known which can distinguish species of this highly diverse group. in this work, we have analyzed the genomes of four actinobacteria (viz. mycobacterium leprae tn, leifsonia xyli subsp. xyli str. ctcb07, bifidobacterium longum ncc2705 and thermobifida fusca yx) to search for proteins that are unique to actinobacteria. our analyses have identified 233 actinobacter ... | 2006 | 16670965 |
| a type ib parb protein involved in plasmid partitioning in a gram-positive bacterium. | our current understanding of segregation of prokaryotic plasmids has been derived mainly from the study of the gram-negative bacterial plasmids. we previously reported a replicon of the cryptic plasmid from a gram-positive bacterium, leifsonia xyli subsp. cynodontis. the replicon contains a putative plasmid partition cassette including a walker-type atpase followed by open reading frame 4 without sequence homologue. here we reported that the orf4 gene was essential for maintaining the plasmid st ... | 2006 | 16997970 |
| construction of a stable expression vector for leifsonia xyli subsp. cynodontis and its application in studying the effect of the bacterium as an endophytic bacterium in rice. | to study the possibility of utilizing genetically engineered leifsonia xyli subsp. cynodontis (lxc) as an endophytic bacterium in rice, we constructed an escherichia coli-lxc shuttle vector, plgus, containing a beta-glucuronidase reporter gene, which was stable both in vitro and in vivo. lxc grows and expresses the beta-glucuronidase reporter gene in all parts of rice, except for seed. a 2-year field study using three rice varieties from china showed that lxc inoculation did not have a negative ... | 2007 | 17166228 |
| heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in ni-rich serpentine soil and in the rhizosphere of alyssum murale. | forty-six bacterial cultures, including one culture collection strain, thirty from the rhizosphere of alyssum murale and fifteen from ni-rich soil, were tested for their ability to tolerate arsenate, cadmium, chromium, zinc, mercury, lead, cobalt, copper, and nickel in their growth medium. the resistance patterns, expressed as minimum inhibitory concentrations, for all cultures to the nine different metal ions were surveyed by using the agar dilution method. a large number of the cultures were r ... | 2007 | 17276484 |
| characterization of the variants, flanking genes, and promoter activity of the leifsonia xyli subsp. cynodontis insertion sequence is1237. | we performed a comprehensive study of the distribution and function of an insertion sequence (is) element, is1237, in the genome of leifsonia xyli subsp. cynodontis, a useful genetic carrier for expressing beneficial foreign genes in plants. two shorter is1237 isoforms, is1237d1 and is1237d2 resulting from precise deletion between two nonperfect repeats, were found in the bacterial genome at a level that was one-fifth the level of wild-type is1237. both the genome and native plasmid pcxc100 harb ... | 2007 | 17293427 |
| humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost. | a bacterial strain isolated from sewage sludge compost, strain sc-083t, was characterized. the isolate was a motile, gram-positive, short rod, forming coryneform v-shaped cells during the early stages of growth. the organism was strictly aerobic and able to grow between 22 and 36 degrees c and between ph 5.5 and 8.0. the predominant fatty acids were cyclohexyl-c17 : 0, anteiso-c17 : 0 and iso-c16 : 0, the major respiratory quinones were menaquinone 11 (mk-11) and 12 (mk-12), and the genomic dna ... | 2008 | 18398211 |
| characterization of new is elements and studies of their dispersion in two subspecies of leifsonia xyli. | leifsonia xyli is a xylem-inhabiting bacterial species comprised of two subspecies: l. xyli subsp. xyli (lxx) and l. xyli subsp. cynodontis (lxc). lxx is the causal agent of ratoon stunting disease in sugarcane commercial fields and lxc colonizes the xylem of several grasses causing either mild or no symptoms of disease. the completely sequenced genome of lxx provided insights into its biology and pathogenicity. since is elements are largely reported as an important source of bacterial genome di ... | 2008 | 18655699 |
| a novel toxin-antitoxin operon tala/b from the gram-positive bacterium leifsonia xyli subsp. cynodontis. | here we report a toxin-antitoxin (ta) operon talab identified from the gram-positive bacterium leifsonia xyli subsp. cynodontis. it is shown that talb encodes a broad-host cytotoxin functioning in different gram-positive bacteria, while tala encodes its antidote. tala and talb form different hetero-oligomers in vitro; these hetero-oligomers, but not the antitoxin tala, strongly bind to the talab promoter region containing two inverted repeats. this represents a new mechanism of binding the promo ... | 2008 | 18722371 |
| leifsonia kribbensis sp. nov., isolated from soil. | a yellow-pigmented actinobacterium, designated strain msl-13t, was isolated from a soil sample collected from bigeum island, republic of korea, and its taxonomic position was determined by using a polyphasic approach. strain msl-13t showed phenotypic and chemotaxonomic properties consistent with its classification in the genus leifsonia. 16s rrna gene sequence analysis of strain msl-13t with sequences from leifsonia naganoensis db103t, leifsonia aquatica dsm 20146t, leifsonia xyli subsp. cynodon ... | 2009 | 19126716 |
| interactions of plant-beneficial bacteria with the ascomycete coniochaeta ligniaria. | to assess the interactions between coniochaeta ligniaria f/tgf15 obtained from torrefied grass fibers (tgf) and selected bacteria from the same substrate. | 2009 | 19298515 |
| cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system. | soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. we isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. this approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium leifsonia xyli, a plant pathogen that has never been isolated outside the host. | 2009 | 19304839 |
| putative lipoproteins identified by bioinformatic genome analysis of leifsonia xyli ssp. xyli, the causative agent of sugarcane ratoon stunting disease. | summary leifsonia xyli ssp. xyli is the causative agent of ratoon stunting disease, a major cause of economic loss in sugarcane crops. understanding of the biology of this pathogen has been hampered by its fastidious growth characteristics in vitro. however, the recent release of a genome sequence for this organism has allowed significant novel insights. further to this, we have performed a bioinformatic analysis of the lipoproteins encoded in the l. xyli genome. these analyses suggest that lipo ... | 2007 | 20507484 |
| crystal structure and centromere binding of the plasmid segregation protein parb from pcxc100. | plasmid pcxc100 from the gram-positive bacterium leifsonia xyli subsp. cynodontis uses a type ib partition system that includes a centromere region, a walker-type atpase para and a centromere-binding protein parb for stable segregation. however, parb shows no detectable sequence homology to any dna-binding motif. here, we study the parb centromere interaction by structural and biochemical approaches. the crystal structure of the c-terminal dna-binding domain of parb at 1.4 å resolution reveals a ... | 2010 | 21123191 |
| asymmetric biocatalytic reduction of 3,5-bis(trifluoromethyl) acetophenone to (1r)-[3,5-bis(trifluoromethyl)phenyl] ethanol using whole cells of newly isolated leifsonia xyli hs0904. | a novel bacterial strain hs0904 was isolated from a soil sample using 3,5-bis(trifluoromethyl) acetophenone as the sole carbon source. this bacterial isolate can asymmetrically reduce 3,5-bis(trifluoromethyl) acetophenone to (1r)-[3,5-bis(trifluoromethyl)phenyl] ethanol with high enantiometric excess (ee) value. based on its morphological, physiological characteristics, biolog, 16s rdna sequence and phylogenetic analysis, strain hs0904 was identified as leifsonia xyli hs0904. to our knowledge, t ... | 2011 | 21614678 |
| polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.). | common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ... | 2011 | 22159885 |
| [Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut]. | We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping. | 2011 | 21866710 |
| genetics and genomics of the genus amycolatopsis. | actinobacteria are gram-positive filamentous bacteria which contains some of the most deadly human pathogens (mycobacterium tuberculosis, m. leprae, corynebacterium diphtheriae, nocardia farcinica), plant pathogens (streptomyces scabies, leifsonia xyli) along with organisms that produces antibiotic (streptomycetes, amycolatopsis, salinospora). interestingly, these bacteria are equipped with an extraordinary capability of producing antibiotics and other metabolites which have medicinal properties ... | 2016 | 27407288 |
| cloning, expression, and directed evolution of carbonyl reductase from leifsonia xyli hs0904 with enhanced catalytic efficiency. | (r)-[3,5-bis(trifluoromethyl)phenyl] ethanol ((r)-btpe) is a valuable chiral intermediate for the synthesis of antiemetic drug aprepitant and fosaprepitant. a leifsonia xyli hs0904-derived carbonyl reductase (lxcar), an effective biocatalyst for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone (btap) to (r)-btpe, was overexpressed in escherichia coli bl21 (de3). bioinformatics analysis indicated that the amino acid sequence of recombinant lxcar showed 89 % similarity to short-ch ... | 2014 | 24788330 |
| draft genome sequence of leifsonia xyli subsp. xyli strain gdw1. | here, we report the draft genome sequence of leifsonia xyli subsp. xyli strain gdw1, isolated from the stem of badila sugarcane located at the guangdong key laboratory for crops genetic improvement (guanzhou, china), that causes ratoon stunting disease of sugarcane. the de novo genome of leifsonia xyli subsp. xyli was assembled with 48 scaffolds and a g+c content of 67.68%, and contained 2.6 mb bp and 2,838 coding sequences. | 2016 | 27795270 |
| complete genome sequence of plant growth-promoting bacterium leifsonia xyli se134, a possible gibberellin and auxin producer. | leifsonia xyli se134 is a potential plant growth-promoting bacterium isolated from a soil in daegu, republic of korea, which produces large amounts of gibberellin (ga) and indole acetic acid (iaa). in this study, we sequenced the complete genome of l. xyli se134 by the pacific biosciences rsii (pacbio) single molecule real time (smrt) sequencing technology. the genome of l. xyli se134 contains a single chromosome that is 3,596,761bp in length, with 70.2% g+c content. the genome contains 3466 pro ... | 2016 | 27725207 |
| rapid and quantitative detection of leifsonia xyli subsp. xyli in sugarcane stalk juice using a real-time fluorescent (taqman) pcr assay. | ratoon stunting disease (rsd) of sugarcane, one of the most important diseases seriously affecting the productivity of sugarcane crops, was caused by the bacterial agent leifsonia xyli subsp. xyli (lxx). a taqman probe-based real-time quantitative polymerase chain reaction (qpcr) assay was established in this study for the quantification of lxx detection in sugarcane stalk juice. a pair of pcr primers (pat1-qf/pat1-qr) and a fluorogenic probe (pat1-qp) targeting the part1 gene of lxx were used f ... | 2016 | 27725937 |
| allohumibacter endophyticus gen. nov., sp. nov., isolated from the root of wild artemisia princeps (mugwort). | a novel actinobacterium designated strain mwe-a11t was isolated from the root of wild artemisia princeps (mugwort). the isolate was aerobic, gram-stain-positive and short rod-shaped, and the colonies were yellow and circular with entire margin. strain mwe-a11t grew at 15-37 °c and ph 6.0-8.0. the predominant isoprenoid quinones were mk-11 and mk-10. the predominant fatty acids were anteiso-c15:0 and iso-c16:0, and the dna g+c content was 68.8 mol%. the main polar lipids were diphosphatidylglycer ... | 2016 | 26842896 |
| in vitro and in vivo plant growth promoting activities and dna fingerprinting of antagonistic endophytic actinomycetes associates with medicinal plants. | endophytic actinomycetes have shown unique plant growth promoting as well as antagonistic activity against fungal phytopathogens. in the present study forty-two endophytic actinomycetes recovered from medicinal plants were evaluated for their antagonistic potential and plant growth-promoting abilities. twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using dn ... | 2015 | 26422789 |
| isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential. | microorganisms associated with medicinal plants are of interest as the producers of important bioactive compounds. to date, the diversity of culturable endophytic actinomycetes associated with medicinal plants is in its initial phase of exploration. in this study, 42 endophytic actinomycetes were isolated from different organs of seven selected medicinal plants. the highest number of isolates (n = 22, 52.3%) of actinomycetes was isolated from roots, followed by stems (n = 9, 21.4%), leaves (n = ... | 2015 | 25904906 |
| a rapid and visual loop-mediated isothermal amplification assay to detect leifsonia xyli subsp. xyli targeting a transposase gene. | leifsonia xyli subsp. xyli (lxx), causal organism of ratoon stunt (rsd), does not produce any reliable internal or external symptoms on sugarcane. its detection on a large scale is solely based on microscopic and serological methods. these methods require well-equipped laboratories, are time consuming and are not feasible for near-field detection of lxx. in this study, we developed a loop-mediated isothermal amplification (lamp) assay for rapid and sensitive detection of lxx without the use of s ... | 2014 | 25201631 |
| complete genome sequence of leifsonia xyli subsp. cynodontis strain dsm46306, a gram-positive bacterial pathogen of grasses. | we announce the complete genome sequence of leifsonia xyli subsp. cynodontis, a vascular pathogen of bermuda grass. the species also comprises leifsonia xyli subsp. xyli, a sugarcane pathogen. since these two subspecies have genome sequences available, a comparative analysis will contribute to our understanding of the differences in their biology and host specificity. | 2013 | 24201198 |
| development of loop-mediated isothermal amplification for detection of leifsonia xyli subsp. xyli in sugarcane. | ratoon stunt, caused by the xylem-limited coryneform bacterium leifsonia xyli subsp. xyli (lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. based on loop-mediated isothermal amplification (lamp), we developed a method for detecting lxx. the major advantages of the lamp method are visual judgment by color and time saving with only 60 min for identification of lxx and without the need for costly pcr apparatus and gel scanner. in the present study, positive and ne ... | 2013 | 23710444 |
| efficient enantioselective synthesis of (r)-[3,5-bis(trifluoromethyl)phenyl] ethanol by leifsonia xyli cctcc m 2010241 using isopropanol as co-substrate. | (r)-[3,5-bis(trifluoromethyl)phenyl] ethanol is a key chiral intermediate for the synthesis of aprepitant. in this paper, an efficient synthetic process for (r)-[3,5- bis(trifluoromethyl)phenyl] ethanol was developed via the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone, catalyzed by leifsonia xyli cctcc m 2010241 cells using isopropanol as the co-substrate for cofactor recycling. firstly, the substrate and product solubility and cell membrane permeability of biocatalysts were ev ... | 2013 | 23462007 |