| surface arrays on the wall of sporosarcina ureae. | thin sections of the cell wall of sporosarcina ureae revealed two structurally distinct layers: a continuous amorphous zone, approximately 15 nm thick, which was adjacent to the plasma membrane, and an overlying periodic zone, approximately 16 nm thick. sequential triton x-100 and lysozyme treatment of isolated walls produced small fragments of the outer regular structure which allowed high-resolution, negatively stained images suitable for optical diffractometric analysis. these data suggested ... | 1979 | 479103 |
| small, acid-soluble, spore proteins and their genes from two species of sporosarcina. | small, acid-soluble proteins (sasp) of both the alpha/beta- and gamma-type were present in spores of sporosarcina ureae and s. halophila, and three genes encoding alpha/beta-type sasp in these species have been cloned and sequenced. the amino acid sequences of the sporosarcina alpha/beta-type sasp are extremely homologous to those of bacillus sasp, further indicative of the close evolutionary relationship between these genera. | 1990 | 2083842 |
| isolation of sporosarcina ureae from a bronchial biopsy in a child with cystic fibrosis. | | 1990 | 2351147 |
| ammonium assimilation in proteus vulgaris, bacillus pasteurii, and sporosarcina ureae. | no active uptake of ammonium was detected in proteus vulgaris, bacillus pasteurii, and sporosarcina ureae, which indicates that these bacteria depend on the passive diffusion of ammonia across the cell membrane. in p. vulgaris the glutamine synthetase-glutamate synthase (gs-gogat) pathway and glutamate dehydrogenase (gdh) were present, and these enzymes exhibited high affinities for ammonium. in b. pasteurii and s. ureae, however, no gs activity was detected, and gogat activity was only present ... | 1989 | 2570557 |
| survey of metal tolerance in moderately halophilic eubacteria. | the tolerance patterns, expressed as mics, for 250 moderately halophilic eubacteria to 10 heavy metals were surveyed by using an agar dilution method. the moderate halophiles tested included 12 culture collection strains and fresh isolates representative of deleya halophila (37 strains), acinetobacter sp. (24 strains), flavobacterium sp. (28 strains), and 149 moderately halophilic gram-positive cocci included in the genera marinococcus, sporosarcina, micrococcus, and staphylococcus. on the basis ... | 1989 | 2802612 |
| purification and characterization of the nickel-containing multicomponent urease from klebsiella aerogenes. | klebsiella aerogenes urease was purified 1,070-fold with a 25% yield by a simple procedure involving deae-sepharose, phenyl-sepharose, mono q, and superose 6 chromatographies. the enzyme preparation was comprised of three polypeptides with estimated mr = 72,000, 11,000, and 9,000 in a alpha 2 beta 4 gamma 4 quaternary structure. the three components remained associated during native gel electrophoresis, mono q chromatography, and superose 6 chromatography despite the presence of thiols, glycols, ... | 1987 | 3553184 |
| deoxyribonucleic acid base composition of sporosarcina ureae. | | 1968 | 5709646 |
| taxonomy of psychrophilic strains of bacillus. | the morphological and physiological characteristics of 20 isolates of psychrophilic bacillus were compared with 29 strains representing nine species of mesophilic bacillus and 2 strains of sporosarcina ureae to determine the taxonomic position of the psychrophiles. the psychrophiles formed four distinct groups which were sufficiently different from the mesophiles to warrant their designation as new species of bacillus. the names b. psychrosaccharolyticus, b. insolitus, b. globisporus, and b. psy ... | 1967 | 6051360 |
| phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera caryophanon, exiguobacterium, kurthia, and planococcus. | the 16s rrna gene sequences of "bacillus aminovorans" and several species considered to be phylogenetically related to the group 2 bacilli of ash et al. (c. ash, j. a. e. farrow, s. wallbanks, and m. d. collins, lett. appl. microbiol. 13:202-206, 1991) were determined. a comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa (the genera caryophanon, exiguobacterium, kurthia, planococcus), form a phylogenetically disti ... | 1994 | 8123563 |
| levels of small molecules in dormant spores of sporosarcina species and comparison with levels in spores of bacillus and clostridium species. | dormant spores of sporosarcina halophila and sporosarcina ureae contained no detectable atp, significant levels of adp, even higher levels of amp, and a large pool of 3-phosphoglyceric acid, similar to what is found in dormant spores of bacillus and clostridium species. sporosarcina halophila and s. ureae spores also contained significant pools of free amino acids, in particular glutamic acid, as in the case with spores of bacillus but not clostridium species. levels of monovalent and divalent i ... | 1993 | 8467422 |
| [restriction endonuclease sse9i (correction of sse91) from sporosarcina strain sp.9d recognizes the 5'-aatt-3' dna sequence]. | sse91, a type ii restriction endonuclease, has been isolated from sporosarcina species 9d. the recognition sequence and cleavage point of restriction endonuclease sse91 have been determined as 5'-decrease aatt-3'. the new enzyme is an isoschizomer of tsp5091, but its optimal incubation temperature is 55 degrees c and it is inactivated at 65 degrees c for 20 min. | 1998 | 9511140 |
| the enzymatic activity of phosphoglycerate mutase from gram-positive endospore-forming bacteria requires mn2+ and is ph sensitive. | the enzymatic activity of phosphoglycerate mutase (pgm) from three gram-positive endospore-forming bacteria (bacillus subtilis, clostridium perfringens, and sporosarcina ureae) requires mn2+ and is very sensitive to ph; at low concentrations of mn2+, a ph change from 8 to 6 resulted in greater than 30- to 200-fold decreases in the activity of these pgms. however, pgm deactivation at ph 6 was reversed by shifting the enzyme to ph 7 or 8. free mn2+ was not directly involved in pgm catalysis, altho ... | 1998 | 9830105 |
| the putative dna translocase spoiiie is required for sporulation of the symmetrically dividing coccal species sporosarcina ureae. | the spoiiie gene of sporosarcina ureae encodes a 780-residue protein, showing 58% identity to the spoiiie protein of bacillus subtilis, which is thought to be a dna translocase. expression of the s. ureae spoiiie gene is able to restore sporulation in a b. subtilis spoiiie mutant. inactivation of the s. ureae spoiiie gene blocks sporulation of s. ureae at stage iii. within the limits of detection, the sporulation division in s. ureae shows the same symmetry, or near symmetry, as the vegetative d ... | 2000 | 10672183 |
| sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in korea, and transfer of bacillus globisporus (larkin and stokes 1967), bacillus psychrophilus (nakamura 1984) and bacillus pasteurii (chester 1898) to the genus sporosarcina as sporosarcina globispora comb. nov., sporosarcina psychrophila comb. nov. and sporosarcina pasteurii comb. nov., and emended description of th. | a light-orange-coloured, facultatively anaerobic, rod-shaped bacterium (strain sw28t), which was isolated from seawater in korea, was taxonomically studied by a polyphasic approach. this organism formed round terminal endospores in swollen sporangia. the peptidoglycan type is a4alpha, based on l-lys-l-ala-d-asp. the predominant menaquinone is mk-7 and the major fatty acid is ante-c15:0. the g+c content of the dna is 40 mol%. phylogenetic analysis based on 16s rdna sequences showed that strain sw ... | 2001 | 11411676 |
| postdivisional synthesis of the sporosarcina ureae dna translocase spoiiie either in the mother cell or in the prespore enables bacillus subtilis to translocate dna from the mother cell to the prespore. | the differentiation of vegetative cells of bacillus subtilis into spores involves asymmetric cell division, which precedes complete chromosome partitioning. the dna translocase spoiiie is required to translocate the origin distal 70% of the chromosome from the larger mother cell into the smaller prespore, the two cells that result from the division. we have tested the effect of altering the time and location of spoiiie synthesis on spore formation. we have expressed the spoiiie homologue from sp ... | 2003 | 12533463 |
| multiplex pcr screening of soil isolates for novel bacillus-related lineages. | a16s rdna multiplex pcr-based high-throughput protocol is presented to screen bacterial isolates in large amounts for the appearance of novel lineages of bacteria, especially hitherto unknown bacillus relatives. the 16s rdnas of 4224 isolates from a comprehensive cultivation campaign were screened for similarity to predominant uncultured soil bacteria. soil suspensions were plated in serial dilutions on various media. after 2, 4 and 6 weeks, colonies were collected with toothpicks and transferre ... | 2003 | 14529967 |
| application of stereolithographic custom models for studying the impact of biofilms and mineral precipitation on fluid flow. | here we introduce the use of transparent experimental models fabricated by stereolithography for studying the impacts of biomass accumulation, minerals precipitation, and physical configuration of flow paths on liquid flow in fracture apertures. the internal configuration of the models ranged in complexity from simple geometric shapes to those that incorporate replicated surfaces of natural fractures and computationally derived fracture surfaces. high-resolution digital time-lapse imaging was em ... | 2005 | 16332867 |
| electron microscopy of ultrathin sections of sporosarcina ureae. | mazanec, k. (j. e. purkyne university, brno, czechoslovakia), m. kocur, and t. martinec. electron microscopy of ultrathin sections of sporosarcina ureae. j. bacteriol. 90:808-816. 1965.-ultrathin sections of sporosarcina ureae cells were studied by means of electron microscopy. the cell wall consists of several layers and is 340 a thick. the cytoplasm is of globular structure and includes ribosomelike structures, occasional mesosomes, and inclusions not precisely identifiable. the nuclear area h ... | 1965 | 16562085 |
| isolation, gene structure, and comparative analysis of the s-layer gene ssla of sporosarcina ureae atcc 13881. | the surface (s)-layer of sporosarcina ureae strain atcc 13881, a periodic ordered structure with p4 square type symmetry, was recently reported to be an excellent biotemplate for the formation of highly ordered metal clusters. the s-layer is formed by self-assembly of a single subunit, the 116 kda ssla protein. here we report on the isolation and sequence analysis of the ssla gene. the protein sequence reveals a high degree of similarity to the sequences of other s-layer proteins that form self- ... | 2007 | 17242964 |
| sporosarcina koreensis sp. nov. and sporosarcina soli sp. nov., isolated from soil in korea. | two gram-positive, aerobic, spore-forming rods, f73(t) and i80(t), were isolated from upland soil. a phylogenetic analysis of 16s rrna gene sequences placed both isolates within the genus sporosarcina, and showed a sequence similarity of 98.9 % between the two strains and a similarity of approximately 94.6-97.3 % with respect to sporosarcina species with validly published names. the values for dna-dna relatedness between the two isolates and related type strains of the genus sporosarcina were be ... | 2007 | 17684239 |
| sporosarcina saromensis sp. nov., an aerobic endospore-forming bacterium. | two gram-positive, endospore-forming, rod-shaped bacterial strains, hg645(t) and hg711, were respectively isolated from surface water of a brackish lake and sediment of a fishery harbour in japan and were subsequently characterized taxonomically using a polyphasic approach. phylogenetic analyses based on 16s rrna gene sequences showed that strains hg645(t) and hg711 are affiliated phylogenetically to the genus sporosarcina, and they exhibit sequence similarities of 95.7-97.3 % to the type strain ... | 2007 | 17684272 |
| [the sse9i restriction-modification system: organization of genes and comparative analysis of proteins structures]. | a nucleotide sequence was established for the full-length sporosarcina species 9d operon coding for enzymes of type ii restriction-modification system sse9i. these enzymes recognize the tetranucleotide dna sequence 5'-aatt-3'. the operon was shown to consist of three genes that are situated with the order: sse9ic-sse9ir-sse9im and are transcribed in the same direction. these genes encode the control protein (c.sse9i), restriction endonuclease (r.sse9i) and dna-methyltransferase (m.sse9i), respec ... | 2007 | 17685226 |
| [the diversity of alkane degrading bacteria in the enrichments with deep sea sediment of the south china sea]. | deep sea sediment samples of the south sea of china were used for isolation and biodiversity examination of hydrocarbon degrading bacterium. 48 isolates were obtained from the enrichments with hexadecane as the sole carbon sources. among them, 27 isolates were capable of degrading alkane; and 4 could produce biosurfactant significantly as determined by the surface tension measurement. 2 isolates belonging to dietzia maris lowered water surface tension to 33 mn/m. this is the first report about d ... | 2007 | 18062265 |
| tracking heat-resistant, cold-thriving fluid milk spoilage bacteria from farm to packaged product. | control of psychrotolerant endospore-forming spoilage bacteria, particularly bacillus and paenibacillus spp., is economically important to the dairy industry. these microbes form endospores that can survive high-temperature, short-time pasteurization; hence, their presence in raw milk represents a major potential cause of milk spoilage. a previously developed culture-dependent selection strategy and an rpob sequence-based subtyping method were applied to bacterial isolates obtained from environm ... | 2008 | 18292280 |
| antimicrobial activity of tissue and associated bacteria from benthic sea anemone stichodactyla haddoni against microbial pathogens. | associated bacteria from stichodactyla haddoni are found maximum in tentacle tissues than the body tissue. there are eight associated bacterial species viz., alcaligenes sp, corynebacterium sp, aeromonas sp, sporosarcina sp, renibacterium sp, camobacterium sp1, camobacterium sp2 and salinococcus sp were recorded. the culture extracts from the associated bacterial species showed sensitivity against human bacterial and fungalpathogens. however, the hexane tissue extract of sea anemone showed maxim ... | 2007 | 18405113 |
| sporosarcina antarctica sp. nov., a psychrophilic bacterium isolated from the antarctic. | a gram-positive, psychrophilic, rod-shaped bacterium, designated strain n-05(t), was isolated from soil samples collected off king george island, west antarctica (6 degrees 13' 31'' s 5 degrees 57' 08'' w). phylogenetic analysis based on 16s rrna gene sequences showed that strain n-05(t) was related to members of the genus sporosarcina and had highest 16s rrna gene sequence similarity with the type strain of sporosarcina macmurdoensis (98.0%). the temperature range for growth of strain n-05(t) w ... | 2008 | 18768614 |
| the effect of a sporosarcina ureae preparation on tumor cells in vitro. | | 1947 | 18921784 |
| lactose mother liquor as an alternative nutrient source for microbial concrete production by sporosarcina pasteurii. | microbiologically induced calcite precipitation by the bacterium sporosarcina pasteurii (ncim 2477) using the industrial effluent of the dairy industry, lactose mother liquor (lml) as growth medium was demonstrated for the first time in this study. the urease activity and the calcite precipitation by the bacterium was tested in lml and compared with the standard media like nutrient media and yeast extract media. calcite constituted 24.0% of the total weight of the sand samples plugged by s. past ... | 2009 | 19107535 |
| the bioleaching feasibility for pb/zn smelting slag and community characteristics of indigenous moderate-thermophilic bacteria. | the feasibility of recovering metal values and removing hazardous elements from the pb/zn smelting slag using bioleaching technique were studied through a flask experiment, and the community characteristics of the indigenous moderate-thermophilic bacteria in this bioleaching system were also analyzed through a culture-independent restriction fragment length polymorphism (rflp) of 16s rrna genes approach. the results show that more than 80% of al, as, cu, mn, fe and zn in the pb/zn smelting slag ... | 2009 | 19171481 |
| re-examination of the taxonomic position of bacillus silvestris rheims et al. 1999 and proposal to transfer it to solibacillus gen. nov. as solibacillus silvestris comb. nov. | following the transfer of three of the six species enclosed in the original definition of rrna group 2 of bacillus to the genus sporosarcina and two to lysinibacillus, other species of this group, some of which were added later, still await taxonomic revision. in a recent publication, a set of 'core' characteristics was proposed for species to be included in the genus bacillus (kämpfer et al., 2006). except for bacillus silvestris, however, several or none of these properties are available for m ... | 2009 | 19406792 |
| strain improvement of sporosarcina pasteurii for enhanced urease and calcite production. | phenotypic mutants of sporosarcina pasteurii (previously known as bacillus pasteurii) (mtcc 1761) were developed by uv irradiation to test their ability to enhance urease activity and calcite production. among the mutants, bp m-3 was found to be more efficient compared to other mutants and wild-type strain. it produced the highest urease activity and calcite production compared to other isolates. the production of extracellular polymeric substances and biofilm was also higher in this mutant than ... | 2009 | 19408027 |
| description of paenisporosarcina quisquiliarum gen. nov., sp. nov., and reclassification of sporosarcina macmurdoensis reddy et al. 2003 as paenisporosarcina macmurdoensis comb. nov. | in the course of a study of the prokaryotic diversity of a landfill site in chandigarh, india, a strain designated sk 55(t) was isolated and characterized using a polyphasic approach. its 16s rrna gene sequence showed closest similarity (98.3 %) to that of sporosarcina macmurdoensis cms 21w(t). the sequence similarity to strains of other hitherto described species of sporosarcina was less than 95.5 %. strain sk 55(t) contains peptidoglycan of the a4alpha type (l-lys-d-asp), mk-8 and mk-7 as the ... | 2009 | 19502317 |
| isolation of bacteria from the ileal mucosa of tnfdeltaare mice and description of enterorhabdus mucosicola gen. nov., sp. nov. | the diversity of bacteria associated with inflamed mucosa was investigated by culturing ileal samples from tnf(deltaare) mice on a selective medium containing mucin. among eight isolates, two strains (mt1b3 and mt1b8(t)) belonged to bacterial groups not yet cultured from the mouse intestine. whereas strain mt1b3 was identified as a member of the family planococcaceae and is closely related to sporosarcina species and filibacter limicola dsm 13886(t), strain mt1b8(t) was a novel bacterium. based ... | 2009 | 19542111 |
| sporosarcina luteola sp. nov. isolated from soy sauce production equipment in japan. | a gram-variable, spore-forming, motile rod, designated strain y1t, was isolated from the hopper surface of equipment used for soy sauce production. phylogenetic analysis based on 16s rrna gene sequence revealed that y1t is affiliated phylogenetically to the genus sporosarcina, and the strain showed sequence similarities of 95.8-99.2% to those of sporosarcina species with validly published names. the values of dna-dna relatedness between strain y1t and related type strains of the genus sporosarci ... | 2009 | 19590149 |
| sporosarcina contaminans sp. nov. and sporosarcina thermotolerans sp. nov., two endospore-forming species. | the taxonomic positions of two gram-positive, endospore-forming rods, strains ccug 53915(t) and ccug 53480(t), isolated from an industrial clean-room floor and from a human blood sample, respectively, were studied. 16s rrna gene sequence similarity studies revealed that both isolates clearly clustered with sporosarcina species. strain ccug 53915(t) was most closely related to sporosarcina koreensis and sporosarcina soli, showing 99.4 and 99.2 % 16s rrna gene sequence similarities to the type str ... | 2010 | 19667369 |
| molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques. | polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ... | 2010 | 20018377 |
| [diversity of culturable bacteria associated with the sea urchin hemicentrotus pulcherrimus from naozhou island]. | to investigate the diversity of culturable bacteria isolated from the sea urchin hemicentrotus pulcherrimus collected from a tidal flat of naozhou island (20 degrees 52'n - 20 degrees 56'n 110 degrees 33'e - 110 degrees 38'e), leizhou bay, south china sea, china. | 2009 | 20112668 |
| calcite-forming bacteria for compressive strength improvement in mortar. | microbiological calcium carbonate precipitation (mcp) has been investigated for its ability to improve the compressive strength of concrete mortar. however, very few studies have been conducted on the use of calcite-forming bacteria (cfb) to improve compressive strength. in this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. we isolated 4 cfb from 7 environmental concrete structures. using sequence analysis of the 16s rrna gen ... | 2010 | 20467254 |
| retraction for jung et al., int j syst evol microbiol 0 (2010) ijs.0.015370-0v1-ijs.0.015370-0. proposal of lysinibacillus sinduriensis sp. nov., and transfer of bacillus massiliensis and bacillus odysseyi to lysinibacillus as lysinibacillus massiliensis comb. nov. and lysinibacillus odysseyi comb. nov. with emended descriptions of the genus. | a gram-positive, rod-shaped, endospore-forming organism, strain blb-1t, was isolated from the samples of tidal flat sediment in the yellow sea. 16s rrna gene sequence analysis demonstrated that this isolate belongs to the bacillus rrna group 2 that is closely related to bacillus massiliensis (97.4%), bacillus odysseyi (96.7%), lysinibacillus fusiformis (96.2%) and lysinibacillus boronitolerans (95.9%). the phylogenetic distance from any validly described species within the genera caryophanon, sp ... | 2010 | 20525814 |
| l-phenylalanine concentration in blood of phenylketonuria patients: a modified enzyme colorimetric assay compared with amino acid analysis, tandem mass spectrometry, and hplc methods. | phenylketonuria (pku) is an autosomal recessive disorder caused by an impaired conversion of l-phenylalanine (phe) to l-tyrosine, typically resulting from a deficiency in activity of a hepatic and renal enzyme l-phenylalanine hydroxylase. the disease is characterized by an increased concentration of phe and its metabolites in body fluids. | 2010 | 20604724 |
| two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (capsicum annuum l.). | plant growth-promoting rhizobacteria (pgpr) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. some of the pgpr have been reported to be able to enter plant tissues and establish endophytic populations. here, we demonstrated an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against a bacterial pathogen. initially, out of 150 bacterial isolate ... | 2007 | 18051359 |
| bioconversion of l-arabinose and other carbohydrates from plant cell walls to alpha-glucan by a soil bacterium, sporosarcina sp. n52. | a gram-positive bacterium, n52, that produces intracellular glucan from l-arabinose, was isolated from soil and identified as sporosarcina sp. according to rrna gene sequence analysis and physiological/biochemical characterizations. glucan production by n52 increased significantly in the exponential phase of aerobic liquid culture and was maintained at the highest level during the stationary phase, reaching 37.0% of the cell dry weight. the glucan was also produced from other tested sugars origi ... | 2010 | 20728349 |
| diversity of cold-active protease-producing bacteria from arctic terrestrial and marine environments revealed by enrichment culture. | a new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from svalbard, norway. the method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. zobell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. one to three typ ... | 2010 | 20799082 |
| description of chryseomicrobium imtechense gen. nov., sp. nov., a new member of the family planococcaceae. | a gram-positive, rod shaped, yellow coloured, non-motile, non-spore forming, strictly aerobic bacterial strain, designated mw 10t, was isolated from bay of bengal, india and was subjected to a polyphasic taxonomic approach. the 16s rrna gene sequence revealed that strain mw 10t was showed highest similarity with bacillus psychrodurans (96.15%) and bacillus psychrotolerans (96.01%) and also showed less than 96% similarity with members of genus paenisporosarcina, planococcous, sporosarcina and pla ... | 2010 | 20833890 |
| optimum conditions for microbial carbonate precipitation. | the type of bacteria, bacterial cell concentration, initial urea concentration, reaction temperature, the initial ca(2+) concentration, ionic strength, and the ph of the media are some factors that control the activity of the urease enzyme, and may have a significant impact on microbial carbonate precipitation (mcp). factorial experiments were designed based on these factors to determine the optimum conditions that take into consideration economic advantage while at the same time giving quality ... | 2010 | 20947128 |
| electrokinetic remediation and microbial community shift of β-cyclodextrin-dissolved petroleum hydrocarbon-contaminated soil. | electrokinetic (ek) migration of β-cyclodextrin (β-cd), which is inclusive of total petroleum hydrocarbon (tph), is an economically beneficial and environmentally friendly remediation process for oil-contaminated soils. remediation studies of oil-contaminated soils generally prepared samples using particular tphs. this study investigates the removal of tphs from, and electromigration of microbial cells in field samples via ek remediation. both tph content and soil respiration declined after the ... | 2010 | 21052991 |
| effect of calcifying bacteria on permeation properties of concrete structures. | microbially enhanced calcite precipitation on concrete or mortar has become an important area of research regarding construction materials. this study examined the effect of calcite precipitation induced by sporosarcina pasteurii (bp m-3) on parameters affecting the durability of concrete or mortar. an inexpensive industrial waste, corn steep liquor (csl), from starch industry was used as nutrient source for the growth of bacteria and calcite production, and the results obtained with csl were co ... | 2010 | 21104104 |
| mercury resistance in sporosarcina sp. g3. | mercuric reductase (mera) enzyme plays an important role in biogeochemical cycling and detoxification of hg and recently, has also been shown to be useful in clean up of hg-contaminated effluents. present study describes isolation of a heavy metal-resistant isolate of sporosarcina, which could tolerate up to 40, 525, 210, 2900 and 370 μm of cd, co, zn, cr and hg respectively. it was found to reduce and detoxify redox-active metals like cr and hg. the chromate reductase and mera activities in the ... | 2010 | 21181488 |
| biodiversity and characterization of aerobic spore-forming bacteria in surimi seafood products. | the microbial quality and safety of surimi seafood products was assessed by studying the prevalence and biodiversity of aerobic spore-forming bacteria at the beginning and end of shelf life in 100 surimi samples. low levels of total flora and sporulated flora were numerated at the beginning of storage, however, residual spores were detected in the majority of samples during storage. furthermore, for 34 samples, total flora counts>10(4) cfu/g were observed at the end of shelf life which could lea ... | 2010 | 21315981 |
| description of sporosarcina newyorkensis sp. nov. recovered from clinical specimens in the state of new york and from raw cow's milk in belgium. | twelve independent isolates of a gram-positive, endospore-forming rod were recovered from clinical specimens in new york state, u.s.a and from raw milk in flanders, belgium. the 16s rrna gene sequences for all isolates were identical. the closest species with a validly published name, based on 16s rrna gene sequence, is sporosarcina koreensis (97.13% similarity). dna-dna hybridization studies demonstrate that the new isolates belong to a species distinct from their nearest phylogenetic neighbors ... | 2011 | 21421928 |
| microbial metabolism in ice and brine at -5°c. | metabolic activity, but not growth, has been observed in ice at temperatures from -5°c to -32°c. to improve understanding of metabolism in ice, we simultaneously examined various aspects of metabolism ((14) c-acetate utilization, macromolecule syntheses and viability via reduction of ctc) of the glacial isolates sporosarcina sp. b5 and chryseobacterium sp. v3519-10 during incubation in nutrient-rich ice and brine at -5°c for 50 days. measured rates of acetate utilization and macromolecule synthe ... | 2011 | 21535342 |
| effects of environmental factors on microbial induced calcium carbonate precipitation. | aims: to gain an understanding of the environmental factors that affect the growth of the bacterium sporosarcina pasteurii, the metabolism of the bacterium, and the calcium carbonate precipitation induced by this bacterium to optimally implement the biological treatment process, microbial induced calcium carbonate precipitation (micp), in situ. methods and results: soil column and batch tests were used to assess the effect of likely subsurface environmental factors on the micp treatment proces ... | 2011 | 21624021 |
| promiscuity, stability and cold adaptation of a newly isolated acylaminoacyl peptidase. | we report on the characterisation of a member of the acylaminoacyl peptidase family, the first isolated from bacteria. the enzyme was obtained from the psychrophilic bacterium sporosarcina psychrophila and shows the typical features of cold adaptation (low t(m), optimal temperature of 40 °c, poor thermal stability). it was also tested for substrate specificity, effect of metals, temperature dependence and structure stability and revealed promiscuous catalytic activity on at least two chemically ... | 2011 | 21635934 |
| biocontainment of polychlorinated biphenyls (pcbs) on flat concrete surfaces by microbial carbonate precipitation. | in this study, a biosealant obtained from microbial carbonate precipitation (mcp) was evaluated as an alternative to an epoxy-coating system. a bacterium sporosarcina pasteurii strain atcc 11859, which metabolizes urea and precipitates calcite in a calcium-rich environment, was used in this study to generate the biosealant on a pcb-contaminated concrete surface. concrete cylinders measuring 3 in (76.2-ámm) by 6 in (152.4-ámm) were made in accordance with astm c33 and c192 and used for this purpo ... | 2011 | 21696884 |
| production, secretion, and cell surface display of recombinant sporosarcina ureae s-layer fusion proteins in bacillus megaterium. | monomolecular crystalline bacterial cell surface layers (s-layers) have broad application potential in nanobiotechnology due to their ability to generate functional supramolecular structures. here, we report that bacillus megaterium is an excellent host organism for the heterologous expression and efficient secretion of hemagglutinin (ha) epitope-tagged versions of the s-layer protein ssla from sporosarcina ureae atcc 13881. three chimeric proteins were constructed, comprising the precursor, c-t ... | 2012 | 22101038 |
| proposal of chungangia koreensis gen. nov., sp. nov., isolated from a marine sediment of the yellow sea in the republic of korea. | a gram-positive, strictly aerobic, non-spore-forming, rod shaped bacterial strain, cau 9163(t), was isolated from marine sediment collected in the republic of korea and its taxonomic position was investigated using a polyphasic approach. the strain cau 9163(t) grew optimally at 30°c and ph 8.0. phylogenetic analysis based on 16s rrna gene sequences revealed that the strain formed a hitherto unknown lineage within the order bacillales containing the genera planomicrobium, planococcus, sporosarcin ... | 2011 | 21984680 |
| reclassification of bacillus beijingensis and bacillus ginsengi qiu et al., 2009 as bhargavaea beijingensis comb. nov. and bhargavaea ginsengi comb. nov. and emended description of the genus bhargavaea. | we have carried out a polyphasic taxonomic characterization of validly published type strains of species, bacillus beijingensis dsm 19037t and bacillus ginsengi dsm 19038t that were phylogenetically closely related to bhargavaea cecembensis lmg 24411t. all the strains are gram-positive, non-motile, moderately halotolerant and non-spore forming. 16s rrna gene sequence analyses showed that these constituted a coherent cluster, with sequence similarities between 99.7 and 98.7 %. the percentage simi ... | 2011 | 22155760 |
| Cultivable bacteria from ancient algal mats from the McMurdo Dry Valleys, Antarctica. | The McMurdo Dry Valleys in Antarctica are a favorable location for preservation of dormant microbes due to their persistent cold and dry climate. In this study, we examined cultivable bacteria in a series of algal mat samples ranging from 8 to 26539 years old. Cultivable bacteria were found in all samples except one (12303 years old), but abundance and diversity of cultivable bacteria decreased with increasing sample age. Only members of the Actinobacteria, Bacteroidetes, and Firmicutes were fou ... | 2012 | 22065250 |
| in vitro and in vivo assessment of intraintestinal bacteriotherapy in chronic kidney disease. | chronic kidney disease may progress to end-stage renal disease, which requires dialysis or kidney transplantation. no generally applicable therapies to slow progression of renal disease are available. bacteriotherapy affords a promising approach to mitigate uremic intoxication by ingestion of live microbes able to catabolize uremic solutes in the gut. the present study evaluates the nonpathogenic soil-borne alkalophilic urease-positive bacterium sporosarcina pasteurii (sp) as a potential urea-ta ... | 2006 | 16436893 |
| immobilized cofactor derivatives for kinetic-based enzyme capture strategies: direct coupling of nad(p)+. | this study reevaluates the potential for direct coupling of nad(p)(+) to a carboxylate-terminating spacer arm using carbodiimide-promoted coupling in an attempt to develop a greatly simplified synthetic method for cofactor immobilization that would support the more widespread adoption of kinetic-based enzyme capture (kbec) strategies for protein purification applications and protein-detecting arrays/proteomic studies. direct coupling of nad(+) to epoxy (1,4-butanediol diglycidyl ether)-activated ... | 2004 | 15556571 |
| sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the mcmurdo dry valleys, antarctica. | strain cms 21w(t) was isolated from a cyanobacterial mat sample taken from a pond located in the mcmurdo dry valleys, antarctica. based on its phenotypic, chemotaxonomic and phylogenetic properties, strain cms 21w(t) was identified as a member of the genus sporosarcina: at the 16s rrna gene level, cms 21w(t) exhibited about 93-96 % similarity to all reported species of sporosarcina and exhibited a maximum similarity of 96 % to both sporosarcina globispora and sporosarcina psychrophila. based on ... | 2003 | 13130019 |
| protease from sporosarcina sp. rrlj 1. | protease was isolated from sporosarcina rrlj1 which was collected from acid tea (camellia sinensis) plantations. it showed potential for production of the enzyme for commercial purposes. the study revealed that optimum ph for growth of the organism was 6.5-7 and supplement of casein (1%) in the medium was required for production of protease. enzyme production and enzyme activity was maximum in 72 hr old broth culture. maximum activity of the enzyme was found at ph 6.5. | 2000 | 10927879 |
| synthesis of allysine ethylene acetal using phenylalanine dehydrogenase from thermoactinomyces intermedius. | allysine ethylene acetal [(s)-2-amino-5-(1,3-dioxolan-2-yl)-pentanoic acid (2)] was prepared from the corresponding keto acid by reductive amination using phenylalanine dehydrogenase (pdh) from thermoactinomyces intermedius atcc 33205. glutamate, alanine, and leucine dehydrogenases, and pdh from sporosarcina species (listed in order of increasing effectiveness) also gave the desired amino acid but were less effective. the reaction requires ammonia and nadh. nad produced during the reaction was r ... | 2000 | 10713207 |
| [restriction endonuclease sse9i from sporosarcina sp. strain 9d recognizes the 5'-aatt-3' dna sequence]. | a new restriction endonuclease sse9i was isolated from the bacterial strain sporosarcina sp. 9d. the enzyme belongs to type ii restrictases and recognizes the tetranucleotide sequence 5'-aatt-3'. the enzyme cleaves dna before the first adenine residue, so it is a true isoschizomer of tsp509i restrictase. however, unlike the prototype, sse9i digests dna at 55 degrees c and loses its activity after 20 min storage at 65 degrees c. | 1998 | 9567290 |
| nucleotide sequence of the sspe genes coding for gamma-type small, acid-soluble spore proteins from the round-spore-forming bacteria bacillus aminovorans, sporosarcina halophila and s. ureae. | the single sspe genes coding for gamma-type small, acid-soluble spore proteins (sasp) of three round-spore-forming bacteria, bacillus aminovorans, sporosarcina halophila and s. ureae, have been cloned and sequenced. while the deduced amino acid sequences of these three gamma-type sasp show clear homology to those from six bacillus species that do not form round spores, there are no residues conserved completely among the 9 sequences known. in addition, the 139 residue b. aminovorans protein is 3 ... | 1998 | 9540829 |
| the division during bacterial sporulation is symmetrically located in sporosarcina ureae. | immunofluorescence microscopy was used to visualize the ftsz band that marks the site of septation in sporosarcina ureae. image analysis indicated that the vegetative division was symmetrically located with respect to the ends of the cells. fusions of lacz to the sporulation loci, spolla and cote, of bacillus subtilis were introduced into s. ureae by mobilization of plasmids containing the fusions from escherichia coli. the fusions showed similar patterns of sporulation-associated expression in ... | 1997 | 9350865 |
| cloning and characterization of sse9i dna-methyltransferase recognizing 5'-aatt-3'. | the gene from sporosarcina species 9d encoding sse9i dna-methyltransferase (m.sse9i) was cloned and expressed in escherichia coli. the recombinant plasmid pmsse-1 contains the m.sse9i gene 1086 bp in length, corresponding to a protein of 362 amino acid residues. m.sse9i recognizes the tetranucleotide sequence 5'-aatt-3' and modifies the second adenine within the recognition sequence. the amino acid sequence of m.sse9i was compared with those of other methylases. according to mutual positions of ... | 1996 | 8759012 |
| phylogenetic characterization of a novel salt-tolerant bacillus species: description of bacillus dipsosauri sp. nov. | the taxonomic position of a novel halophilic endospore-forming bacterium previously isolated from a desert iguana was investigated by 16s rrna gene sequencing. comparative sequence analyses showed the unidentified bacterium to be phylogenetically loosely associated with some other spore-forming (bacillus pantothenticus, sporosarcina halophila) and non-spore-forming (marinococcus albus) halotolerant bacteria. based on the phenotypic and phylogenetic distinctiveness of the unidentified bacterium, ... | 1996 | 8675481 |
| cell division in sporosarcina ureae. | electron microscopy of thin-sectioned and freeze-etched cells of sporosarcina ureae demonstrated that division occurred in two or three perpendicular planes. the complex wall consisted of a 12-nm-thick amorphous layer which was adjacent to the plasma membrane (pm) and an overlying 16-nm-thick poroteinaceous array (a regularly structured (rs) layer). septation proceeded with the ingrowth of the pm and the inner layer in association with division mesosomes. the rs layer remained behind at the init ... | 1980 | 7407705 |
| sequence characterization of 5s ribosomal rna from eight gram positive procaryotes. | the available comparative data on procaryotic 5s rrna was extended through sequencing studies of eight gram positive procaryotes. complete nucleotide sequences were presented for 5s rrna from bacillus subtilis, b. firmus, b. pasteurii, b. brevis, lactobacillus brevis and streptococcus faecalis. in addition, 5s rrna oligonucleotide catalogs and partial sequence data were provided for b. cereus and sporosarcina ureae. these sequences and catalogs were discussed in terms of known features of procar ... | 1976 | 823342 |
| phylogenetic analysis of the genera planococcus, marinococcus and sporosarcina and their relationships to members of the genus bacillus. | a phylogenetic analysis based on 16s rrna was performed on the genera planococcus, marinococcus, sporosarcina and endospore-forming rods. in agreement with earlier 16s rrna cataloguing data, planococcus citreus and sporosarcina ureae clustered with bacillus pasteurii and other bacilli containing lysine in their cell walls. sporosarcina halophila was shown to be genetically distinct from s. ureae and formed a loose association with the main bacillus subtilis grouping. marinococcus halophilus (for ... | 1992 | 1505740 |
| characterization of urease from sporosarcina ureae. | alkaline stable (ph 7.75-12.5) urease from sporosarcina ureae was purified over 400-fold by ion exchange and hydrophobic interaction chromatography. the cytoplasmic enzyme was remarkably active with a specific activity of greater than 9300 mumol urea degraded min-1 mg protein-1 at ph 7.5, where it has optimal activity. although s. ureae is closely related to bacillus pasteurii, known to possess a homopolymeric urease containing 1 nickel per subunit [m(r) = 65000], the s. ureae enzyme is comprise ... | 1992 | 1510567 |
| thermostable phenylalanine dehydrogenase of thermoactinomyces intermedius: cloning, expression, and sequencing of its gene. | the gene encoding the thermostable phenylalanine dehydrogenase [ec 1.4.1.-] of a thermophile, thermoactinomyces intermedius, was cloned and its complete dna sequence was determined. the phenylalanine dehydrogenase gene (pdh) consists of 1,098 nucleotides and encodes 366 amino acid residues corresponding to the subunit (mr 41,000) of the hexameric enzyme. the amino acid sequence deduced from the nucleotide sequence of the pdh gene of t. intermedius was 56.0 and 42.1% homologous to those of the ph ... | 1991 | 1880121 |
| 16s ribosomal rna analysis of filibacter limicola indicates a close relationship to the genus bacillus. | the phylogenetic relationship of the gram-negative, filamentous gliding bacterium filibacter limicola was analysed by 16s rrna oligonucleotide cataloguing. in contrast to the proposed membership of this asporogenous species in the flexibacteriaceae, filibacter limicola clusters phylogenetically with the gram-positive eubacteria bacillus pasteurii, sporosarcina ureae and the asporogenous species planococcus citreus. the genetic relationship is supported by several common phenotypic properties. | 1985 | 2415672 |
| comparative 16s rrna oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives. | the phylogenetic incoherency of the genus bacillus as presently described is demonstrated by analysis of both published and new data from comparative 16s rrna cataloguing of nine bacillus species and a number of related non-bacillus taxa, i.e. caryophanon latum, filibacter limicola and planococcus citreus. while the ellipsoidal-spore-forming bacilli, e.g. b. subtilis and allied species, formed a coherent cluster, the round-spore-forming bacilli showed a higher degree of relationship to the non-s ... | 1987 | 2452227 |
| novel phenylalanine dehydrogenases from sporosarcina ureae and bacillus sphaericus. purification and characterization. | nad+-dependent phenylalanine dehydrogenases were purified 1,500- and 1,600-fold, and crystallized from sporosarcina ureae scrc-r04 and bacillus sphaericus scrc-r79a, respectively. the purified enzymes were homogeneous as judged by disc gel electrophoresis. the enzyme from s. ureae has a molecular weight of 305,000, while that of b. sphaericus has a molecular weight of 340,000. each is probably composed of eight subunits identical in molecular weight. the s. ureae enzyme showed a high substrate s ... | 1987 | 3112142 |
| nucleotide sequences of 5s ribosomal rnas of planococcus citreus, planococcus kocurii and sporosarcina ureae. | | 1988 | 3194204 |
| three-dimensional structure of the tetragonal surface layer of sporosarcina ureae. | the three-dimensional structure of the regular surface layer of sporosarcina ureae has been determined to a resolution of 1.7 nm by electron microscopy and image reconstruction. the s-layer has p4 symmetry, a lattice constant of 12.9 nm, and a minimum thickness of 6.6 nm. the reconstruction reveals a distinct domain structure: a massive core, arms connecting adjacent unit cells, and spurs which make contact at the subsidiary fourfold symmetry axes. in the z-direction the domains appear to be arr ... | 1986 | 3759908 |
| biosynthesis and cross-linking of the gamma-glutamylglycine-containing peptidoglycan of vegetative cells of sporosarcina ureae. | | 1974 | 4822501 |
| comparative allostery of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthetase as a molecular basis for classification. | the allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. the implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organisms, sporosarcina ureae and aeromonas formicans, the taxonomic positions of which have been historically controversial. s. ureae has characteristics of both bacillaceae and microco ... | 1970 | 4985590 |
| structure of the regular surface layer of sporosarcina ureae. | optical diffraction and computer image processing of electron micrographs were employed to analyze the structure of the regular surface layer of sporosarcina ureae at high resolution. negatively stained preparations of regular surface layer fragments showed two types of tetragonal pattern, each having p4 symmetry in projection with a = 12.8 nm. although the two patterns differed greatly in overall appearance, both had a common pattern of areas of high stain density which we interpret as arising ... | 1980 | 7372574 |
| immobilization of phenylalanine dehydrogenase and its application in flow-injection analysis system for determination of plasma phenylalanine. | phenylalanine dehydrogenase (l-phedh) from sporosarcina ureae was immobilized on deae-cellulose, modified initially with 2-amino-4,6-dichloro-s-triazine followed by hexamethylenediamine and glutaraldehyde. the highest activity of immobilized phedh was determined as 95.75 u/g support with 56% retained activity. the optimum ph value of immobilized l-phedh was shifted from ph 10.4 to 11.0. the immobilized l-phedh showed activity variations close to the maximum value in a wider temperature range of ... | 2011 | 20658209 |
| Description of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus massiliensis and Bacillus odysseyi to Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended description of the genus. | A Gram-positive, rod-shaped, endospore-forming bacterium, strain BLB-1T, was isolated from the samples of tidal flat sediment in the Yellow Sea. The 16S rRNA gene sequence analysis demonstrated that the isolate belonged to the Bacillus rRNA group 2 and it was closely related to Bacillus massiliensis CIP 108446T (97.4%), Bacillus odysseyi ATCC PTA-4993T (96.7%), Lysinibacillus fusiformis DSM 2898T (96.2%), and Lysinibacillus boronitolerans DSM 17140T (95.9%). Sequence similarities with species in ... | 2011 | 22140163 |
| biomineralization based remediation of as(iii) contaminated soil by sporosarcina ginsengisoli. | arsenic is a highly toxic metalloid and has posed high risk to the environment. as(iii) is highly mobile in soil and leached easily into groundwater. the current remediation techniques are not sufficient to immobilize this toxic element. in the present study, an as(iii) tolerant bacterium sporosarcina ginsengisoli cr5 was isolated from as contaminated soil of urumqi, china. we investigated the role of microbial calcite precipitated by this bacterium to remediate soil contaminated with as(iii). t ... | 2011 | 22154871 |