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clostridium ramosum and beta hemolytic streptococci isolated from a child presenting with acute otitis media. 1979387327
beta-lactamase activity in anaerobic bacteria.a diverse group of anaerobic bacteria representing a spectrum of resistance to beta-lactam antibiotics was studied to characterize their beta-lactamase activity and relate it to resistance. the bacteroides fragilis organisms had moderate resistance and produced a cephalosporinase with low activity. the clostridium ramosum was intermediate in resistance and had intermediate, inducible beta-lactamase activity. the b. clostridiiformis organism was highly resistant, produced a potent inducible penic ...1976984742
resistance to beta-lactam antibiotics in anaerobic bacteria.the known mechanisms of beta-lactam resistance in anaerobic bacteria involve production of beta-lactamases, alteration of penicillin-binding proteins, and blocked penetration of beta-lactam antibiotics through bacterial outer membranes. the most important factor in beta-lactam resistance is production of beta-lactamases, which have been found in various bacteroides, fusobacterium, and clostridium species. beta-lactam resistance in bacteroides fragilis is commonly mediated by beta-lactamases that ...19902406875
interference of iga protease with the effect of secretory iga on adherence of oral streptococci to saliva-coated hydroxyapatite.it has previously been shown that secretory immunoglobulin a (s-iga) influences the sorption of oral streptococci to hydroxyapatite as well as to cell surfaces. the present experiments demonstrate that bacterial iga proteases, which cleave s-iga in the hinge region, are capable of interfering with this mechanism. this result was obtained with an iga1 specific protease from haemophilus influenzae and with a protease from clostridium ramosum that cleaves iga1 as well as iga2 of a2m(1) allotype. th ...19873040826
resistance of normal serum iga and secretory iga to bacterial iga proteases: evidence for the presence of enzyme-neutralizing antibodies in both serum and secretory iga, and also in serum igg.normal serum iga and secretory iga (siga) of subclass iga1 were isolated from pooled human serum and milk, respectively. they were tested for their susceptibility to bacterial iga proteases from haemophilus influenzae, streptococcus pneumoniae, neisseria gonorrhoeae, and neisseria meningitidis that cleave iga of only the iga1 subclass. they were also tested for susceptibility to a novel iga-protease from clostridium ramosum that cleaves iga of the iga1 as well as the iga2 subclass of the a2m(1) ...19873127662
menaquinone production and utilization in germ-free rats after inoculation with specific organisms.although menaquinones are biologically active forms of vitamin k, factors that influence their production by bacteria or their absorption from the gut are not well understood. germ-free rats were inoculated with four different strains of organisms and fecal and tissue menaquinone concentrations were determined. no menaquinones were detected in the tissues or feces of rats colonized with bifidobacterium longum or clostridium ramosum, two organisms that have not been reported to produce menaquinon ...19873298580
deconjugation of bile acids by human intestinal bacteria implanted in germ-free rats.fecal bile acids in germ-free rats were analyzed after inoculation with bacteroides vulgatus, bifidobacterium longum, escherichia coli or clostridium ramosum. b. vulgatus preferentially deconjugated tauro-beta-muricholic acid and b. longum taurocholic acid. c. ramosum deconjugated both bile acids, but e. coli deconjugated neither. 7 alpha-dehydroxylation of bile acids was negligible even after 18 days of inoculation, but a small amount of 7-oxo-bile acids, less than 5%, was formed. fecal excreti ...19873312906
iga protease from clostridium ramosum that cleaves iga1 and iga2, a2m(1): the site of cleavage and digestion of secretory iga. 19873321935
emphysematous pyelonephritis in a xanthogranulomatous kidney. an unusual cause of pneumoperitoneum.emphysematous pyelonephritis is a rare, life-threatening suppurative infection of the renal parenchyma and perinephric tissues. the disease is encountered primarily in patients with diabetes mellitus or ureteral obstruction associated with perinephric and intrarenal gas. causative organisms are those normally found in the urinary and gastrointestinal tracts; however, anaerobic bacteria have been demonstrated in only 1% of cases. we describe a case of emphysematous pyelonephritis, which presented ...19883341917
in vitro activity of imipenem against gram-positive anaerobic bacteria.the in vitro activity of imipenem, a new penem antibiotic, was determined against 210 clinical gram-positive anaerobic isolates and compared with the activities of metronidazole, clindamycin, cefoxitin, moxalactam, ceftizoxime, ceftriaxone and cefotiam. all investigated strains were inhibited by a 4-mg/l concentration of imipenem. cefoxitin demonstrated good activity against most strains with exception of some clostridium difficile and clostridium ramosum strains. cephalosporins were classed in ...19873473047
mechanisms of beta-lactam resistance in anaerobic bacteria.the known mechanisms of beta-lactam resistance in anaerobic bacteria involve production of beta-lactamases, alteration of penicillin-binding proteins, and blocking of the penetration of beta-lactams through the outer membranes. the most important factor in beta-lactam resistance is the production of beta-lactamase. beta-lactamases in various bacteroides, fusobacterium, and clostridium species have been described. beta-lactam resistance in bacteroides fragilis is most commonly mediated by the pro ...19863541135
effect of moxalactam on human fecal microflora.five healthy male adults received 2 g of moxalactam every 12 h for 7 days. the alterations of fecal microflora were investigated before, during, and after treatment with moxalactam. on day 7 of treatment, the number of total bacteria was decreased in all subjects. there was marked suppression of the obligate anaerobic bacteria and enterobacteria to undetectable levels, but the counts of streptococcus spp. and lactobacillus spp. increased. on day 7, two subjects had clostridium innocuum and clost ...19863729329
clostridium ramosum, an iga protease-producing species and its ecology in the human intestinal tract.a bacterial strain isolated from feces of a patient with ulcerative colitis, which had been shown to produce a novel immunoglobulin a (iga) protease (cleaving both the human iga1 subclass and iga2 subclass of a2m(1) allotype) extracellularly, was identified as clostridium ramosum. by using a selective medium (propionate-rifampicin-gentamicin-colimycin-polymyxin medium) devised for c. ramosum, analysis of the population level of this organism was performed to determine its ecology in the human in ...19853912649
detection of bacteroides fragilis and bacteroides melaninogenicus by direct immunofluorescence.a new diagnostic kit, which contains a polyvalent antiserum for either bacteroides fragilis or bacteroides melaninogenicus, was tested for reliability and specificity on 146 clinical samples of different origin. a correlation between the culture and immunofluorescence was observed for b. fragilis in 87.39% of cases and for b. melaninogenicus in 81.48% of cases. when pure cultures were tested, aerobically as well as anaerobically, false-positive reactions were observed with staphylococci and clos ...19806108969
the survival of anaerobic bacteria at 4 degrees c and 22 degrees c on swabs in three transport systems. statistical evaluation by application of a variance component model.the survival of anaerobic bacteria on swabs in three transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), a plain cotton swab in a dry aerobic tube (da) and a plain cotton swab in an aerobic agar tube (aa). the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum and clostridium ra ...19836346789
the recovery of anaerobic bacteria from swabs in three transport systems. statistical evaluation by application of a variance component model.the recovery of six anaerobic species from swabs in three transport systems was quantitatively evaluated. the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum, and clostridium ramosum. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), or a plain cotton swab in either a dry aerobic tube (da) or an aerobic agar tube (aa). swabs were charged ...19836346790
left ventricular mass with septic (clostridium ramosum) arterial emboli in a renal allograft patient: report of a case and review of the literature. 19836349055
[characterization of species of the genus clostridium by gas chromatographic analysis of the fatty acids produced during metabolism].volatile and non volatile fatty acids deriving from the bacterial metabolism of different sugars were determined by gas-chromatography to better characterize clostridium tertium and clostridium ramosum. the data obtained were also used for numerical taxonomic analysis and dendrograms were elaborated to study the taxonomic relationships between the two species.19846536545
characterization of lactobacillus sp. strain 100-37 from the murine gastrointestinal tract: ecology, plasmid content, and antagonistic activity toward clostridium ramosum h1.a gram-positive, nonsporulating, microaerophilic rod that had two colonial variants was obtained during a study in which anaerobic bacteria were isolated from murine gastrointestinal tracts and screened for cryptic plasmids. the rod (both colonial variants) was identified as a lactobacillus sp. (strain 100-37) by selective media, gas chromatography, and biochemical tests. in monoassociated, ex-germfree mice, the bacterium colonized the gastrointestinal tract and formed a thick, continuous layer ...19836651295
techniques for controlling variability in gram staining of obligate anaerobes.identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ...19957538512
delta 22-beta-muricholic acid in monoassociated rats and conventional rats.bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ...19948177022
how far should a clinical laboratory go in identifying anaerobic isolates, and who should pay?identification of anaerobic bacteria in specimens from sites of infection due to mixed organisms can be time-consuming and expensive. laboratories should limit anaerobic workups by testing only those specimens that have been properly collected and transported to the laboratory. use of selective and differential media for initial processing can provide rapid and relevant information to the clinician. anaerobes isolated from normally sterile sites and sites of serious infection should always be co ...19938324163
identification and antimicrobial resistance patterns of clinical isolates of clostridium clostridioforme, clostridium innocuum, and clostridium ramosum compared with those of clinical isolates of clostridium perfringens.clostridium ramosum, c. innocuum, and c. clostridioforme are frequently isolated from clinical specimens including blood. because of gram stain variability, a lack of spores, and atypical colonial morphology, identification of these species is often difficult. three anaerobe identification kits were evaluated for their abilities to identify these species. for comparison, 11 strains of c. perfringens were evaluated in parallel. by using profile numbers and codebooks, the correct genus and species ...19958586704
analysis of the specificity of bacterial immunoglobulin a (iga) proteases by a comparative study of ape serum igas as substrates.immunoglobulin a (iga) proteases are bacterial enzymes with substrate specificity for human serum and secretory igas. to further define the basis of this specificity, we examined the ability of iga proteases of clostridium ramosum, streptococcus pneumoniae (ec 3.4.24.13), neisseria meningitidis (ec 3.4.21.72), and haemophilus influenzae (ec 3.4.21.72) to cleave serum igas of gorillas, chimpanzees, and orangutans. all enzymes cleaved the igas of the three apes despite differences in ape iga1 hing ...19968641803
stimulation of transforming growth factor beta1 by enteric bacteria in the pathogenesis of rat intestinal fibrosis.bacteria and their products stimulate inflammatory responses. certain mediators, such as transforming growth factor beta1 (tgf-beta1), induce collagen synthesis. excess collagen deposition results in bowel strictures. the aim of this study was to investigate the role of bacteria and tgf-beta1 in the pathogenesis of intestinal fibrosis.19989496942
[clostridium ramosum: a rare cause of brain abscess]. 19989710995
in vitro activity of gemifloxacin (sb 265805) against anaerobes.gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...199910471570
microbes involved in dissimilatory nitrate reduction in the human large intestine.nitrate-limited batch cultures, incorporating 20 different fermentation substrates and inoculated with human faeces, mainly selected for the growth of enterobacteria. the microbial diversity involved was determined by a combination of phenotypic and genotypic procedures. continuous culture with lactate as the sole electron donor selected for similar micro-organisms, but when antibiotics were incorporated to inhibit escherichia coli and lactate was replaced with choline, there was a wider microbi ...200010620715
coprobacillus catenaformis gen. nov., sp. nov., a new genus and species isolated from human feces.three strains of eubacterium-like isolates from human feces were characterized by biochemical tests and 16s rdna analysis. the phenotypic characteristics of the three strains resembled those of the genus collinsella transferred from the genus eubacterium recently. however, eubacterium-like strains were phylogenetically members of the clostridium subphylum of gram-positive bacteria, and these showed a specific phylogenetic association with clostridium ramosum and c. spiroforme. c. ramosum and c. ...200010711596
characterization of antibody responses against rectal mucosa-associated bacterial flora in patients with ulcerative colitis.previous reports on faecal microflora have demonstrated that the total number of aerobes and coliforms was increased in patients with ulcerative colitis (uc). based on the hypothesis that the pathogenesis of uc may be closely associated with the mucosal microflora, we investigated alterations in the mucosa-associated microflora of uc patients.200010719749
gas gangrene in an immunocompromised girl due to a clostridium ramosum infection. 199910825071
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...200111120995
in vitro activity of telithromycin (hmr 3647) against 502 strains of anaerobic bacteria.in a previous study, we compared hmr 3004 with azithromycin, clarithromycin, erythromycin and roxithromycin against 502 anaerobic bacteria using nccls-approved procedures. this report extends this study by reporting the activity of telithromycin (hmr 3647) against these strains. telithromycin inhibited 10% of bacteroides fragilis, 50% of other b. fragilis group organisms and 93% of other bacteroides spp. telithromycin inhibited all porphyromonas spp. and 98% of prevotella spp. activity against b ...200111266423
antibacterial activity of fifth-generation dentin bonding systems.past concepts that the pulp does not become infected until an actual carious exposure takes place have been challenged. the antibacterial effects of the dentin bonding systems single bond, prime&bond nt, and excite were evaluated using the bacteria streptococcus mutans atcc 25175, streptococcus intermedius, lactobacillus acidophilus, prevotella oris, prevotella bivia, prevotella denticola, porphyromonas gingivalis, porphyromonas endodontalis, and clostridium ramosum with a disk diffusion method. ...200111771577
the clostridium ramosum iga proteinase represents a novel type of metalloendopeptidase.clostridium ramosum is part of the normal flora in the human intestine. some strains produce an iga proteinase that specifically cleaves human iga1 and the iga2m(1) allotype. this prolylendopeptidase was purified from a broth culture supernatant, and n-terminal sequences of the native protein and tryptic fragments thereof were determined. a fragment of the iga gene encoding the iga proteinase was isolated using degenerate primers in pcr, and the complete gene was obtained by inverse pcr. the ide ...200211815614
in vitro activities of daptomycin, vancomycin, quinupristin- dalfopristin, linezolid, and five other antimicrobials against 307 gram-positive anaerobic and 31 corynebacterium clinical isolates.the activities of daptomycin, a cyclic lipopeptide, and eight other agents were determined against 338 strains of gram-positive anaerobic bacteria and corynebacteria by the nccls reference agar dilution method with supplemented brucella agar for the anaerobes and mueller-hinton agar for the corynebacteria. the daptomycin mics determined on ca(2+)-supplemented (50 mg/liter) brucella agar plates were one- to fourfold lower than those determined in unsupplemented media. daptomycin was highly active ...200312499210
antimicrobial properties of self-etching primer-bonding systems.self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ...200312670069
spondylodiscitis due to clostridium ramosum infection in an immunocompetent elderly patient.the first ever case of spondylodiscitis caused by clostridium ramosum in an elderly immunocompetent patient has been reported. c. ramosum is usually an intestinal bacterium but may occasionally be isolated in clinical specimens as an opportunistic pathogen. this report shows that this anaerobic organism can cause bone tropism without there having been any contamination due to spinal surgery. the infection cleared after empirical therapy using intravenous amoxicillin and oral metronidazole.200312734285
in vitro activities of the new semisynthetic glycopeptide telavancin (td-6424), vancomycin, daptomycin, linezolid, and four comparator agents against anaerobic gram-positive species and corynebacterium spp.telavancin is a new semisynthetic glycopeptide anti-infective with multiple mechanisms of action, including inhibition of bacterial membrane phospholipid synthesis and inhibition of bacterial cell wall synthesis. we determined the in vitro activities of telavancin, vancomycin, daptomycin, linezolid, quinupristin-dalfopristin, imipenem, piperacillin-tazobactam, and ampicillin against 268 clinical isolates of anaerobic gram-positive organisms and 31 corynebacterium strains using agar dilution meth ...200415155214
in vitro activities of opt-80 and comparator drugs against intestinal bacteria.the activities of opt-80 against 453 intestinal bacteria were compared with those of seven other drugs. opt-80 showed good activity against most clostridia, staphylococci, and enterococci, but streptococci, aerobic and facultative gram-negative rods, anaerobic gram-negative rods, and clostridium ramosum were resistant. poor activity against anaerobic gram-negative rods may maintain colonization resistance.200415561877
secondary infection of intracranial hydatid cyst with clostridium ramosum.introduction: brain involvement in hydatid disease occurs in 1-2% of all echinococcus granulosus infections. secondary infection of intracranial hydatid cysts is extremely rare. case report and discussion: in this case report, we present a secondary infection of an intracranial hydatid cyst due to clostridium ramosum, which is an extremely rare infectious pathogen in neurosurgical practice, and a potential pitfall in neuroradiological investigations.200415580512
susceptibility of clostridium ramosum to antimicrobial agents.the susceptibility of 49 strains of clostridium ramosum to 10 antibiotics was determined by agar dilution and disk diffusion tests. results showed that, among the anaerobes, c. ramosum is second only to bacteroides fragilis in its resistance to antimicrobial agents. all strains were susceptible to penicillin, carbenicillin, chloramphenicol, vancomycin, and metronidazole at readily achievable blood levels. most strains (83%) were susceptible to erythromycin. there was a high level of resistance t ...197415825410
lung abscess caused by clostridium ramosum.a 49-year-old male presented with weight loss and fever. further evaluation revealed lung abscesses and clostridium ramosum bacteremia. this is an unusual cause of infection. there have only been seven reports of infections caused by c. ramosum.200616703936
identification of clostridium species and dna fingerprinting of clostridium perfringens by amplified fragment length polymorphism analysis.an amplified fragment length polymorphism (aflp) method was applied to 129 strains representing 24 different clostridium species, with special emphasis on pathogenic clostridia of medical or veterinary interest, to assess the potential of aflp for identification of clostridia. in addition, the ability of the same aflp protocol to type clostridia at the strain level was assessed by focusing on clostridium perfringens strains. all strains were typeable by aflp, so the method seemed to overcome the ...200616971642
clostridium saccharogumia sp. nov. and lactonifactor longoviformis gen. nov., sp. nov., two novel human faecal bacteria involved in the conversion of the dietary phytoestrogen secoisolariciresinol diglucoside.two anaerobic bacteria involved in the conversion of the plant lignan secoisolariciresinol diglucoside were isolated from faeces of a healthy male adult. the first isolate, strain sdg-mt85-3db, was a mesophilic strictly anaerobic gram-positive helically coiled rod. based on 16s r rna gene sequence analysis, its nearest relatives were clostridium cocleatum (96.7% similarity) and clostridium ramosum (96.6%). in contrast to these species, the isolate was devoid of alpha-galactosidase and -glucosida ...200717196483
clostridium ramosum in a case of cerebellar abscess.a case of cerebellar abscess due to clostridium ramosum is reported here. the microbiological workup has been discussed.200117664819
epidemiology of clostridium species bacteremia in calgary, canada, 2000-2006.to define the incidence, risk factors for acquisition, and outcomes associated with clostridial bacteremia in a large canadian health region.200818672296
comparative in vitro activity of rep3123 against clostridium difficile and other anaerobic intestinal bacteria.the aim of this study was to determine the anaerobic spectrum of activity of rep3123, a novel diaryldiamine that inhibits bacterial methionyl-trna synthetases in gram-positive bacteria.200919240076
molecular inventory of faecal microflora in patients with crohn's disease.intestinal microbial community is involved in the pathogenesis of crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. using sequence analysis of randomly cloned bacterial 16s ribosomal dna, the dominant faecal species from four crohn's disease patients and four controls were compared. whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly re ...200419712374
effect of carbohydrate composition in barley and oat cultivars on microbial ecophysiology and proliferation of salmonella enterica in an in vitro model of the porcine gastrointestinal tract.the influence of the carbohydrate (cho) composition of cereal cultivars on microbial ecophysiology was studied using an in vitro model of the porcine gastrointestinal tract. ten hull-less barley cultivars, six barley cultivars with hulls, six oat cultivars, and six oat groats that differed in beta-glucan, nonstarch polysaccharide (nsp), and starch contents and starch type were hydrolyzed enzymatically and incubated for 72 h with pig feces. fermentation kinetics were modeled, and microbial compos ...200919783749
human intestinal microbiota: characterization of a simplified and stable gnotobiotic rat model.the study of host microbe interactions is hampered by the complexity and inter-individual variability of the human gut microbiota. therefore, a simplified human intestinal microbiota (sihumi) consisting of seven bacterial species was introduced into germfree rats. species selection was based on numerical importance and fermentative abilities in the human gut. association of the rats with the sihumi (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, blautia producta, clos ...201021637015
clostridium ramosum promotes high-fat diet-induced obesity in gnotobiotic mouse models.the intestines of obese humans and mice are enriched with erysipelotrichi, a class within the firmicutes. clostridium ramosum, a member of the erysipelotrichi, is associated with symptoms of the metabolic syndrome in humans. to clarify the possible obesogenic potential of this bacterial species and to unravel the underlying mechanism, we investigated the role of c. ramosum in obesity development in gnotobiotic mice. mice were associated with a simplified human intestinal (sihumi) microbiota of e ...201425271283
bacteriostatic effect of orally administered bovine lactoferrin on proliferation of clostridium species in the gut of mice fed bovine milk.when milk-fed mice were orally inoculated with clostridium ramosum c1, this strain proliferated in the gut and became the dominant component of the fecal microflora. in this experimental model, bovine lactoferrin (blf) administered with milk suppressed the proliferation of this strain in vivo and decreased the numbers of c. ramosum and other bacteria in the feces. this bacteriostatic effect of blf was dependent on the concentration of blf, the duration of feeding, and the administered dose of c. ...19957574587
effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria.the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ...19807431377
the intestinal microbiota in metabolic disease.gut bacteria exert beneficial and harmful effects in metabolic diseases as deduced from the comparison of germfree and conventional mice and from fecal transplantation studies. compositional microbial changes in diseased subjects have been linked to adiposity, type 2 diabetes and dyslipidemia. promotion of an increased expression of intestinal nutrient transporters or a modified lipid and bile acid metabolism by the intestinal microbiota could result in an increased nutrient absorption by the ho ...201627058556
evaluation of impact of exposure of sudan azo dyes and their metabolites on human intestinal bacteria.sudan azo dyes are banned for food usage in most countries, but they are illegally used to maintain or enhance the color of food products due to low cost, bright staining, and wide availability of the dyes. in this report, we examined the toxic effects of these azo dyes and their potential reduction metabolites on 11 prevalent human intestinal bacterial strains. among the tested bacteria, cell growth of 2, 3, 5, 5, and 1 strains was inhibited by sudan i, ii, iii, iv, and para red, respectively. ...201222634331
comparative in vitro activities of lff571 against clostridium difficile and 630 other intestinal strains of aerobic and anaerobic bacteria.the in vitro activities of lff571, a novel analog of ge2270a that inhibits bacterial growth by binding with high affinity for protein synthesis elongation factor tu, fidaxomicin, and 10 other antimicrobial agents were determined against 50 strains of clostridium difficile and 630 other anaerobic and aerobic organisms of intestinal origin. lff571 possesses potent activity against c. difficile and most other gram-positive anaerobes (mic(90), ≤ 0.25 μg/ml), with the exception of bifidobacteria and ...201222290948
antibacterial activity against clostridium genus and antiradical activity of the essential oils from different origin.in the present study, the antimicrobial and antiradical activities of 15 essential oils were investigated. the antimicrobial activities were determined by using agar disc diffusion and broth microdilution methods against clostridium genus and antioxidant properties of essential oils by testing their scavenging effect on dpph radicals activities. we determined the antibacterial activity of clostridium butyricum, clostridium hystoliticum, clostridium intestinale, clostridium perfringens and clostr ...201424813985
septic arthritis due to clostridium ramosum.clostridium species are anaerobic bacilli that are rarely reported as etiologic agents of infectious arthritis. previous cases of arthritis caused by clostridium ramosum have not been reported. we describe the first 2 cases of c. ramosum arthritis.201626546506
flx pyrosequencing analysis of the effects of the brown-algal fermentable polysaccharides alginate and laminaran on rat cecal microbiotas.edible brown algae are used as major food material in far east asian countries, particularly in south korea and japan. they contain fermentable dietary fibers, alginic acid (uronic acid polymer) and laminaran (β-1,3-glucan), that are fermented into organic acids by intestinal bacteria. to clarify the effect of edible algae on the intestinal environment, the cecal microbiotas of rats fed diets containing no dietary fiber (control) or 2% (wt/wt) sodium alginate or laminaran for 2 weeks were analyz ...201323183985
[a preliminary analysis of changes in composition of intestinal microbiota during infancy using polymerase chain reaction-denaturing gradient gel electrophoresis].to investigate the composition of bacteria in the stools of infants and the colonization of intestinal microbiota during infancy.201728302207
synthesis of β-galactooligosaccharide using bifidobacterial β-galactosidase purified from recombinant escherichia coli.galactooligosaccharides (gos) are known to be selectively utilized by bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. in this study, β-gos are synthesized using bifidobacterial β-galactosidase(g1) purified from recombinant e. coli with a high gos yield and with high productivity and enhanced bifidogenic activity. the purified recombinant g1 showed maximum production of β-gos at ph 8.5 and 45°c. a matrix-assisted laser desorption ionization time ...201728535609
isolation of genetically tractable most-wanted bacteria by metaparental mating.metagenomics has rapidly advanced our inventory and appreciation of the genetic potential inherent to the gut microbiome. however it is widely accepted that two key constraints to further genetic dissection of the gut microbiota and host-microbe interactions have been our inability to recover new isolates from the human gut, and the paucity of genetically tractable gut microbes. to address this challenge we developed a modular rp4 mobilisable recombinant vector system and an approach termed meta ...201526293474
clostridium ramosum bacteremia: case report and literature review.clostridium ramosum is a common enteric anaerobe but infrequently also a cause of pathologic infection.201424283763
comparison of the antibacterial effect of modified 3-mix paste versus ultrapex over anaerobic microorganisms from infected root canals of primary teeth: an in vitro study.the aim of this study was to evaluate in vitro the antimicrobial efficacy of a modified 3-mix paste and to compare it with an iodoform paste (ultrapex) against anaerobic microorganisms isolated from root canals of infected or necrotic primary teeth.201222838224
non-autoimmune hemolytic anemia with clostridium ramosum bacteremia. 200824179346
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