| [mesophilic clostridia from soil of the central plateau of costa rica]. | thirty soil samples from the central plateau of costa rica yielded 710 isolates of 34 clostridia species. these were clostridium lituseburense, c. oceanicum and c. sardiniense (100, 73, and 60%, respectively). the toxigenic species of the genus were present in at least one sample, except for c. botulinum, c. chauvoei and c. spiroforme. diversity per sample (4-12 species) exceeded what has been reported in similar studies. no correlation has been found between the presence of a species and soil c ... | 1993 | 7701076 |
| phylogeny of the ammonia-producing ruminal bacteria peptostreptococcus anaerobius, clostridium sticklandii, and clostridium aminophilum sp. nov. | in previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. three isolates, strains c, ft (t = type strain), and sr, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. on the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. in this study, the 16s rrna sequences of these isolat ... | 1993 | 8427801 |
| variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16s rrna-targeted oligonucleotide probes. | six 16s rrna-targeted oligonucleotide probes were designed, validated, and used to quantify predominant groups of anaerobic bacteria in human fecal samples. a set of two probes was specific for species of the bacteroides fragilis group and the species bacteroides distasonis. two others were designed to detect species of the clostridium histolyticum and the clostridium lituseburense groups. another probe was designed for the genera streptococcus and lactococcus, and the final probe was designed f ... | 1998 | 9726880 |
| mucosal and invading bacteria in patients with inflammatory bowel disease compared with controls. | endogenous intestinal bacteria and/or specific bacterial pathogens are suspected of being involved in the pathogenesis of inflammatory bowel diseases (ibd). the aim of this study was to investigate ibd tissues for different bacterial population groups harbouring the mucosal surface and/or invading the mucosa. | 2002 | 12374228 |
| identification of bacterial populations in dairy wastewaters by use of 16s rrna gene sequences and other genetic markers. | hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. although these systems are common, little is known about the microbial populations that inhabit them or how they change as they traverse the system. using culture-based and non-culture-based methods, we characterized the microbial community structure of manure, water from the separator p ... | 2004 | 15240310 |
| effects of difructose anhydride iii (dfa iii) administration on rat intestinal microbiota. | the effects of difructose anhydride iii (di-d-fructofuranose-1,2':2,3'-dianhydride; dfa iii) administration (3% dfa iii for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (dgge). according to dgge profiles, the number of bacteria related to bacteroides acidofaciens and uncultured bacteria within the clostridium lituseburense group decreased, while that of bacteria related to bacteroides vulgatus, bacteroides uniformis and ruminococcus productus ... | 2005 | 16233782 |
| prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria. | the differences in faecal bacterial population between irritable bowel syndrome (ibs) and control subjects have been reported in several studies. the aim of the present study was to compare the predominant and clostridial faecal microbiota of ibs subjects and healthy controls by applying denaturing gradient gel electrophoresis (dgge) and a recently developed multiplexed and quantitative hybridization-based technique, transcript analysis with the aid of affinity capture (trac). according to the r ... | 2006 | 16585652 |
| molecular monitoring of the fecal microbiota of healthy human subjects during administration of lactulose and saccharomyces boulardii. | diet is a major factor in maintaining a healthy human gastrointestinal tract, and this has triggered the development of functional foods containing a probiotic and/or prebiotic component intended to improve the host's health via modulation of the intestinal microbiota. in this study, a long-term placebo-controlled crossover feeding study in which each subject received several treatments was performed to monitor the effect of a prebiotic substrate (i.e., lactulose), a probiotic organism (i.e., sa ... | 2006 | 16957220 |
| imbalance in the composition of the duodenal microbiota of children with coeliac disease. | coeliac disease (cd) is the most common immune-mediated enteropathy characterized by chronic inflammation of the small intestinal mucosa. the ingestion of gluten is responsible for the symptoms of cd, but other environmental factors are also thought to play a role in this disorder. in this study, the composition of the duodenal microbiota of coeliac children with active disease, symptom-free cd patients on a gluten-free diet and control children was determined. bacteriological analyses of duoden ... | 2007 | 18033837 |
| effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects. | diet influences the composition of the gut microbiota and host's health, particularly in patients suffering from food-related diseases. coeliac disease (cd) is a permanent intolerance to cereal gluten proteins and the only therapy for the patients is to adhere to a life-long gluten-free diet (gfd). in the present preliminary study, the effects of a gfd on the composition and immune function of the gut microbiota were analysed in ten healthy subjects (mean age 30.3 years) over 1 month. faecal mic ... | 2009 | 19445821 |
| effect of a new synbiotic mixture on atopic dermatitis in infants: a randomized-controlled trial. | clinical trials investigating the therapeutic effect of probiotics on atopic dermatitis (ad) show inconsistent results. better results can possibly be achieved by combining probiotics with prebiotics, i.e. synbiotics. | 2010 | 20184604 |
| intestinal microbiota in allergic and nonallergic 1-year-old very low birth weight infants after neonatal glutamine supplementation. | previously, glutamine-enriched enteral nutrition in very low birth weight infants (vlbw) decreased the incidence of atopic dermatitis at age 1 year. the aim of this study was to determine whether this effect is related to changes in intestinal bacterial species that are associated with allergy, such as bifidobacteria, clostridium histolyticum, clostridium lituseburense (chis/lit group) and escherichia coli at age 1 year. | 2010 | 20626364 |
| [identification and metabolism characterization of a clostridium lituseburense strain isolated from high-altitude soil]. | we studied the physiological, biochemical properties and metabolism of clostridium lituseburense p4-1 from soil in namucuo. | 2010 | 21090252 |
| specific prebiotics modulate gut microbiota and immune activation in haart-naive hiv-infected adults: results of the "copa" pilot randomized trial. | intestinal mucosal immune system is an early target for human immunodeficiency virus type 1 (hiv-1) infection, resulting in cd4(+) t-cell depletion, deterioration of gut lining, and fecal microbiota composition. we evaluated the effects of a prebiotic oligosaccharide mixture in highly active antiretroviral therapy (haart)-naive hiv-1-infected adults. in a pilot double-blind, randomized, placebo-controlled study, 57 haart-naive hiv-1-infected patients received a unique oligosaccharide mixture (15 ... | 2011 | 21525866 |
| Effects of pH and ORP on microbial ecology and kinetics for hydrogen production in continuously dark fermentation. | The microbial structure and kinetic characteristics of the hydrogen producing strains in two fermentative continuous stirred-tank reactors (CSTRs) were studied by controlling pH and oxidation and reduction potential (ORP). The fluorescence in situ hybridization (FISH) tests were conducted to investigate the fermentative performance of Clostridium histolyticum (C. histolyticum), Clostridium lituseburense (C. lituseburense) and Enterobacteriaceae. The experimental results showed that in ethanol-ty ... | 2011 | 21978625 |
| correlation between protection against sepsis by probiotic therapy and stimulation of a novel bacterial phylotype. | prophylactic probiotic therapy has shown beneficial effects in an experimental rat model for acute pancreatitis on the health status of the animals. mechanisms by which probiotic therapy interferes with severity of acute pancreatitis and associated sepsis, however, are poorly understood. the aims of this study were to identify the probiotic-induced changes in the gut microbiota and to correlate these changes to disease outcome. duodenum and ileum samples were obtained from healthy and diseased r ... | 2011 | 21926217 |
| effect of prebiotics on the fecal microbiota of elderly volunteers after dietary supplementation of bacillus coagulans gbi-30, 6086. | in advancing age, gut populations of beneficial microbes, notably bifidobacterium spp., show a marked decline. this contributes to an environment less capable of maintaining homoeostasis. this in vitro investigation studied the possible synergistic effects of probiotic supplementation in modulating the gut microbiota enabling prebiotic therapy to in elderly persons. single stage batch culture anaerobic fermenters were used and inoculated with fecal microbiota obtained from volunteers after takin ... | 2014 | 25219857 |
| the cellular lipids of romboutsia. | we have examined the lipids of three isolates, romboutsia lituseburensis, romboutsia ilealis, and romboutsia sp. strain frifi, of the newly described genus romboutsia by two-dimensional thin-layer chromatography (2d-tlc) and by liquid chromatography/mass spectrometry (lc/ms). we have found three phospholipids, phosphatidylglycerol (pg), cardiolipin and phosphatidic acid in all three species. a fourth phospholipid, lysyl-pg, was found in r. lituseburensis and strain frifi. polyprenyl-phosphates w ... | 2016 | 27317428 |
| characterization of romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus clostridium into the genera romboutsia gen. nov., intestinibacter gen. nov., terrisporobacter gen. nov. and asaccharospora gen. nov. | a gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated cribt, was isolated from the gastro-intestinal tract of a rat and characterized. the major cellular fatty acids of strain cribt were saturated and unsaturated straight-chain c12-c19 fatty acids, with c16:0 being the predominant fatty acid. the polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. the ... | 2014 | 24480908 |
| whole-genome sequence of clostridium lituseburense l74, isolated from the larval gut of the rhinoceros beetle, trypoxylus dichotomus. | clostridium lituseburense l74 was isolated from the larval gut of the rhinoceros beetle, trypoxylus dichotomus collected in yeong-dong, chuncheongbuk-do, south korea and subjected to whole genome sequencing on hiseq platform and annotated on rast. the nucleotide sequence of this genome was deposited into ddbj/embl/genbank under the accession nz_litj00000000. | 2016 | 26981432 |
| cultivation-independent comprehensive survey of bacterial diversity in tulsi shyam hot springs, india. | a taxonomic description of bacteria was deduced from 5.78 mb metagenomic sequence retrieved from tulsi shyam hot spring, india using bacterial tag-encoded flx amplicon pyrosequencing (btefap). metagenome contained 10,893 16s rdna sequences that were analyzed by mg-rast server to generate the comprehensive profile of bacteria. metagenomic data are available at ebi under ebi metagenomics database with accession no. erp009559. metagenome sequences represented the 98.2% bacteria origin, 1.5% of euka ... | 2015 | 26484176 |
| [anaerobic bacteria involved in the degradation of aromatic sulfonates to methane]. | an anaerobic microbial consortium that degrades benzene- and p-toluenesulfonate to form methane and fatty acids has been produced. pure cultures of three strains of anaerobic spore-forming bacteria clostridium spp., as well as the sulfate-reducing bacteria desulfovibrio sp., were isolated and characterized. phylogenetic analysis of 16s rrna gene sequences of strains showed that pure cultures of clostridia strains 14, 24, and 21 are close to clostridium lituseburense dsm 797t, c. sartagoforme dsm ... | 2016 | 26027356 |
| romboutsia sedimentorum sp. nov., isolated from an alkaline-saline lake sediment and emended description of the genus romboutsia. | a gram-stain-positive, spore-forming, obligately anaerobic bacterium, designated lam201(t), was isolated from sediment samples from an alkaline-saline lake located in daqing oilfield, daqing city, pr china. cells of strain lam201(t) were non-motile and straight or spiral rod-shapes. strain lam201(t) was able to utilize glucose, fructose, maltose, trehalose and sorbitol as the sole carbon source. acetic acid, ethanol, iso-butanoic acid and iso-valeric acid were the main products of glucose fermen ... | 2015 | 25609678 |
| characterization of a potentially novel 'blown pack' spoilage bacterium isolated from bovine hide. | to characterize a psychrotrophic bacterium, designated tc1, previously isolated from a cattle hide in ireland, and to investigate the ability of this strain to cause 'blown pack' spoilage (bps) of vacuum-packaged beef primals. | 2013 | 23163427 |